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https://www.readbyqxmd.com/read/29679179/isolation-and-characterization-of-phosphofungi-and-screening-of-their-plant-growth-promoting-activities
#1
Xiaohui Wang, Changdong Wang, Junkang Sui, Zhaoyang Liu, Qian Li, Chao Ji, Xin Song, Yurong Hu, Changqian Wang, Rongbo Sa, Jiamiao Zhang, Jianfeng Du, Xunli Liu
Rhizospheric microorganisms can increase phosphorus availability in the soil. In this regard, the ability of phosphofungi to dissolve insoluble phosphorus compounds is greater than that of phosphate-solubilizing bacteria. The aim of the current study was to identify efficient phosphofungi that could be developed as commercial microbial agents. Among several phosphate-solubilizing fungal isolates screened, strain CS-1 showed the highest phosphorus-solubilization ability. Based on phylogenetic analysis of the internal transcribed spacer region sequence, it was identified as Aspergillus niger...
April 20, 2018: AMB Express
https://www.readbyqxmd.com/read/29677705/in-house-validation-and-small-scale-collaborative-study-to-evaluate-analytical-performances-of-multi-mycotoxin-screening-methods-based-on-liquid-chromatography-high-resolution-mass-spectrometry-case-study-fusarium-toxins-in-wheat
#2
Biancamaria Ciasca, Michelangelo Pascale, Valerio Guido Altieri, Francesco Longobardi, Michele Suman, Dante Catellani, Veronica M T Lattanzio
A strong trend toward using highly selective mass spectrometry technologies for screening of multiple mycotoxins has been observed in recent years. In the present study, the process of validation of a multi-mycotoxin screening method based on liquid chromatography high-resolution mass spectrometry method is presented. The method was intended for the simultaneous screening of the major Fusarium toxins (deoxynivalenol, 3- and 15-acetyl deoxynivalenol, T-2 and HT-2 toxins, zearalenone, enniatins A, A1, B, B1, beauvericin) in wheat...
April 20, 2018: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/29673318/genome-wide-analysis-of-family-1-udp-glycosyltransferases-ugt-and-identification-of-ugt-genes-for-fhb-resistance-in-wheat-triticum-aestivum-l
#3
Yi He, Dawood Ahmad, Xu Zhang, Yu Zhang, Lei Wu, Peng Jiang, Hongxiang Ma
BACKGROUND: Fusarium head blight (FHB), a devastating disease in wheat worldwide, results in yield loses and mycotoxin, such as deoxynivalenol (DON), accumulation in infected grains. DON also facilitates the pathogen colonization and spread of FHB symptoms during disease development. UDP-glycosyltransferase enzymes (UGTs) are known to contribute to detoxification and enhance FHB resistance by glycosylating DON into DON-3-glucoside (D3G) in wheat. However, a comprehensive investigation of wheat (Triticum aestivum) UGT genes is still lacking...
April 19, 2018: BMC Plant Biology
https://www.readbyqxmd.com/read/29673315/inter-genome-comparison-of-the-quorn-fungus-fusarium-venenatum-and-the-closely-related-plant-infecting-pathogen-fusarium-graminearum
#4
Robert King, Neil Andrew Brown, Martin Urban, Kim E Hammond-Kosack
BACKGROUND: The soil dwelling saprotrophic non-pathogenic fungus Fusarium venenatum, routinely used in the commercial fermentation industry, is phylogenetically closely related to the globally important cereal and non-cereal infecting pathogen F. graminearum. This study aimed to sequence, assemble and annotate the F. venenatum (strain A3/5) genome, and compare this genome with F. graminearum. RESULTS: Using shotgun sequencing, a 38,660,329 bp F. venenatum genome was assembled into four chromosomes, and a 78,618 bp mitochondrial genome...
April 19, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29660386/long-term-cryopreservation-of-non-spore-forming-fungi-in-microbank%C3%A2-beads-for-plant-pathological-investigations
#5
Dilip K Lakshman, Vimla Singh, Manuel E Camacho
Long-term preservation of experimental fungi without genetic, morphological, and pathogenic changes is of paramount importance in mycological and plant pathological investigations. Several cryogenic and non-cryogenic methods are available for the preservation of fungi, but the methods can be cumbersome, hazardous, expensive, and often not suitable for long-term storage of non-spore-forming (sterile) fungi. A method of preservation of spore-forming fungi in commercially available porous beads (Micrbank™) under cryogenic condition was successfully tested for three non-spore-forming basidiomycetes genera: Rhizoctonia solani (teleomorph: Thanatephorus cucumeris) (n = 19), Ceratobasidium species (n = 1), and Waitea circinata (n = 3), and a non-spore forming ascomycetes, Sclerotinia sclerotiorum (n = 1)...
April 13, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29651174/identification-and-pathogenicity-of-fungal-pathogens-causing-black-point-in-wheat-on-the-north-china-plain
#6
Kai-Ge Xu, Yu-Mei Jiang, Yang-Kun Li, Qiao-Qiao Xu, Ji-Shan Niu, Xin-Xin Zhu, Qiao-Yun Li
Fungi associated with black point were isolated from three highly susceptible wheat genotypes in the North China Plain. The 21 isolates represented 11 fungal genera. The most prevalent genera were Alternaria (isolation frequency of 56.7%), Bipolaris (16.1%), and Fusarium (6.0%). The other eight genera were Curvularia , Aspergillus , Cladosporium , Exserohilum , Epicoccum , Nigrospora , Penicillium , and Ulocladium ; their isolation frequencies ranged from 0.8 to 4.8%. The pathogenicity of the isolates was individually assessed in the greenhouse by inoculating wheat plants with spore suspensions...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29642627/a-review-of-the-interactions-between-wheat-and-wheat-pathogens-zymoseptoria-tritici-fusarium-spp-and-parastagonospora-nodorum
#7
REVIEW
Adrian Duba, Klaudia Goriewa-Duba, Urszula Wachowska
Zymoseptoria tritici is a hemibiotrophic pathogen which causes Septoria leaf blotch in wheat. The pathogenesis of the disease consists of a biotrophic phase and a necrotrophic phase. The pathogen infects the host plant by suppressing its immune response in the first stage of infection. Hemibiotrophic pathogens of the genus Fusarium cause Fusarium head blight, and the necrotrophic Parastagonospora nodorum is responsible for Septoria nodorum blotch in wheat. Cell wall-degrading enzymes in plants promote infections by necrotrophic and hemibiotrophic pathogens, and trichothecenes, secondary fungal metabolites, facilitate infections caused by fungi of the genus Fusarium ...
April 10, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29621291/fungal-community-profiles-in-agricultural-soils-of-a-long-term-field-trial-under-different-tillage-fertilization-and-crop-rotation-conditions-analyzed-by-high-throughput-its-amplicon-sequencing
#8
Loreen Sommermann, Joerg Geistlinger, Daniel Wibberg, Annette Deubel, Jessica Zwanzig, Doreen Babin, Andreas Schlüter, Ingo Schellenberg
Fungal communities in agricultural soils are assumed to be affected by soil and crop management. Our intention was to investigate the impact of different tillage and fertilization practices on fungal communities in a long-term crop rotation field trial established in 1992 in Central Germany. Two winter wheat fields in replicated strip-tillage design, comprising conventional vs. conservation tillage, intensive vs. extensive fertilization and different pre-crops (maize vs. rapeseed) were analyzed by a metabarcoding approach applying Illumina paired-end sequencing of amplicons generated by two recently developed primer pairs targeting the two fungal Internal Transcribed Spacer regions (ITS1, ITS2)...
2018: PloS One
https://www.readbyqxmd.com/read/29577312/rapid-prediction-of-deoxynivalenol-contamination-in-wheat-bran-by-mos-based-electronic-nose-and-characterization-of-the-relevant-pattern-of-volatile-compounds
#9
Vincenzo Lippolis, Salvatore Cervellieri, Anna Damascelli, Michelangelo Pascale, Annalisa Di Gioia, Francesco Longobardi, Annalisa De Girolamo
BACKGROUND: Deoxynivalenol (DON) is the mycotoxin, mainly produced by Fusarium species, most frequently occurring in cereals and cereal-based products. Wheat bran is the outer layers of the kernel with a high risk of chemical hazards, including mycotoxins. Rapid methods for DON detection in wheat bran are highly demanded. RESULTS: A rapid screening method using an electronic nose (e-nose) based on metal oxide semiconductor sensors has been developed to distinguish wheat bran samples with different levels of DON contamination...
March 25, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29574560/in-house-validation-of-a-rapid-and-efficient-procedure-for-simultaneous-determination-of-ergot-alkaloids-and-other-mycotoxins-in-wheat-and-maize
#10
Natalia Arroyo-Manzanares, Karl De Ruyck, Valdet Uka, Laura Gámiz-Gracia, Ana M García-Campaña, Sarah De Saeger, José Diana Di Mavungu
A fundamental step in addressing the global problem of mycotoxins is the development of highly sensitive, multi-class extraction and detection methods. This constitutes a field of research that has in recent years enjoyed a steady advance. Such methods, generally based on liquid chromatography coupled to mass spectrometry, are widely reported successfully detecting various mycotoxins in different food and feed samples. In this work, an innovative approach to multi-class mycotoxin control is proposed, offering specific advantages: a broader inclusion of more mycotoxin classes, robust and thorough extraction for all target compounds despite their varied chemical properties, and determination of all analytes from a single injection...
March 24, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29562133/the-fungicidal-activity-of-tebuconazole-enantiomers-against-fusarium-graminearum-and-its-selective-effect-on-don-production-under-different-conditions
#11
Xue Diao, Yiye Hang, Chenglan Liu
Tebuconazole, which consists of a pair of enantiomers with different fungicidal activities, is one of the most common fungicides used in the control of Fusarium graminearum. In this study, the fungicidal activity of rac-tebuconazole and its enantiomers against F. graminearum was determined at 0.997, 0.975, 0.950 aw and at 20, 25, 30 °C on wheat-based media. Then, F. graminearum treated with rac-tebuconazole and its enantiomers at the EC10, EC50 and EC90 levels under different culture conditions, and DON production was measured...
March 21, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29561710/molecular-mapping-of-fusarium-head-blight-resistance-in-the-spring-wheat-line-nd2710
#12
Mingxia Zhao, Guomei Wang, Yueqiang Leng, Humphrey Wanjugi, Pinggen Xi, Mike Grosz, Mohamed Mergoum, Shaobin Zhong
ND2710 is a hard red spring wheat line with a very high level of resistance to Fusarium head blight (FHB). It was selected from the progeny of a cross between ND2603 (an advanced breeding line derived from the Sumai 3/Wheaton cross) and Grandin (a spring wheat cultivar). The FHB resistance of ND2710 is presumably derived from Sumai 3 since the other parents Grandin and Wheaton are very susceptible to FHB. To identify and map the quantitative trait loci (QTL) for FHB resistance in ND2710, we developed a mapping population consisting of 233 recombinant inbred lines (RILs) from the cross between ND2710 and the spring wheat cultivar 'Bobwhite'...
March 21, 2018: Phytopathology
https://www.readbyqxmd.com/read/29554557/regional-differences-in-the-composition-of-fusarium-head-blight-pathogens-and-mycotoxins-associated-with-wheat-in-mexico
#13
Minely Cerón-Bustamante, Todd J Ward, Amy Kelly, Martha M Vaughan, Susan P McCormick, Christina Cowger, Santos G Leyva-Mir, Héctor E Villaseñor-Mir, Victoria Ayala-Escobar, Cristian Nava-Díaz
Fusarium Head Blight (FHB) is a destructive disease of small grain cereals and a major food safety concern. Epidemics result in substantial yield losses, reduction in crop quality, and contamination of grains with trichothecenes and other mycotoxins. A number of different fusaria can cause FHB, and there are significant regional differences in the occurrence and prevalence of FHB pathogen species and their associated mycotoxins. Information on FHB pathogen and mycotoxin diversity in Mexico has been extremely limited, but is needed to improve disease and mycotoxin control efforts...
May 20, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29551207/isolation-identification-and-the-growth-promoting-effects-of-two-antagonistic-actinomycete-strains-from-the-rhizosphere-of-mikania-micrantha-kunth
#14
Dandan Han, Lanying Wang, Yanping Luo
Actinomycetes are an important group of gram-positive bacteria that play an essential role in the rhizosphere ecosystem. The confrontation culture and Oxford cup method were used to evaluate the antagonistic activities of strains, which were isolated from the rhizosphere soil of Mikania micrantha. The two isolates were identified using morphological and physiological tests combined with 16S rRNA-based molecular analysis, respectively. The type I polyketone synthase (PKS-I) was amplified. The constituents of fermentation metabolites were analyzed by gas chromatography mass spectrometry...
March 2018: Microbiological Research
https://www.readbyqxmd.com/read/29548430/from-malt-to-wheat-beer-a-comprehensive-multi-toxin-screening-transfer-assessment-and-its-influence-on-basic-fermentation-parameters
#15
Kristina Mastanjević, Bojan Šarkanj, Rudolf Krska, Michael Sulyok, Benedikt Warth, Krešimir Mastanjević, Božidar Šantek, Vinko Krstanović
The aim was to determine the mycotoxin transfer rate into beer during a semi-industrial production process and the effect of fungicide treatment in the field on mycotoxins concentrations in beer. To ensure the usual practical agronomical conditions, sample A was treated with fungicide Prosaro® 250, and sample B was infected with Fusarium culmorum spores, in order to obtain infected malt. Malt was produced using standard procedure and beer was produced in a semi-industrial unit. During fermentation measurement of sugars (maltotriose and maltose), glycerol and ethanol content was performed on a daily basis...
July 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29538315/transcriptome-analysis-identifies-a-140-kb-region-of-chromosome-3b-containing-genes-specific-to-fusarium-head-blight-resistance-in-wheat
#16
Xin Li, Shengfu Zhong, Wanquan Chen, Syeda Akash Fatima, Qianglan Huang, Qing Li, Feiquan Tan, Peigao Luo
Fusarium head blight (FHB), mainly caused by Fusarium graminearum , is one of the most destructive fungal diseases of wheat ( Triticum aestivum L.). Because of the quantitative nature of FHB resistance, its mechanism is poorly understood. We conducted a comparative transcriptome analysis to identify genes that are differentially expressed in FHB-resistant and FHB-susceptible wheat lines grown under field conditions for various periods after F. graminearum infection and determined the chromosomal distribution of the differentially expressed genes (DEGs)...
March 14, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29528269/fusarium-algeriense-sp-nov-a-novel-toxigenic-crown-rot-pathogen-of-durum-wheat-from-algeria-is-nested-in-the-fusarium-burgessii-species-complex
#17
Imane Laraba, Abdelaziz Keddad, Houda Boureghda, Nora Abdallah, Martha M Vaughan, Robert H Proctor, Mark Busman, Kerry O'Donnell
A novel crown rot pathogen of wheat discovered during pathogen surveys in Algeria in 2014 and 2015 is formally described as Fusarium algeriense. Multilocus molecular phylogenetic data resolved the eight isolates of this pathogen as a genealogically exclusive species lineage in the F. burgessii species complex. The previously described species of this complex, F. burgessii and F. beomiforme, produce abundant chlamydospores in culture, and their optimal temperature for growth is 30 C. In comparison, F. algeriense did not produce chlamydospores under the conditions tested and its optimal temperature for growth is 25 C...
February 13, 2018: Mycologia
https://www.readbyqxmd.com/read/29518004/host-and-cropping-system-shape-the-fusarium-population-3adon-producers-are-ubiquitous-in-wheat-whereas-niv-producers-are-more-prevalent-in-rice
#18
Meixin Yang, Hao Zhang, Xiangjiu Kong, Theo van der Lee, Cees Waalwijk, Anne van Diepeningen, Jin Xu, Jingsheng Xu, Wanquan Chen, Jie Feng
In recent years, Fusarium head blight (FHB) outbreaks have occurred much more frequently in China. The reduction of burning of the preceding crop residues is suggested to contribute to more severe epidemics as it may increase the initial inoculum. In this study, a large number of Fusarium isolates was collected from blighted wheat spikes as well as from rice stubble with perithecia originating from nine sampling sites in five provinces in Southern China. Fusarium asiaticum dominated both wheat and rice populations, although rice populations showed a higher species diversity...
March 8, 2018: Toxins
https://www.readbyqxmd.com/read/29502265/aquaporin1-regulates-development-secondary-metabolism-and-stress-responses-in-fusarium-graminearum
#19
Mingyu Ding, Jing Li, Xinyue Fan, Fang He, Xiaoyang Yu, Lei Chen, Shenshen Zou, Yuancun Liang, Jinfeng Yu
The Ascomycete fungus Fusarium graminearum, the causal agent of Fusarium head blight of wheat and barley, has become a predominant model organism for the study of fungal phytopathogens. Aquaporins (AQPs) have been implicated in the transport of water, glycerol, and a variety of other small molecules in yeast, plants and animals. However, the role of these proteins in phytopathogenic fungi is not well understood. Here, we identified and attempted to elucidate the function of the five aquaporin genes in F. graminearum...
March 3, 2018: Current Genetics
https://www.readbyqxmd.com/read/29478269/tolerance-of-triazole-based-fungicides-by-biocontrol-agents-used-to-control-fusarium-head-blight-in-wheat-in-argentina
#20
J M Palazzini, A M Torres, S N Chulze
Fusarium head blight (FHB) caused by Fusarium graminearum species complex is a devastating disease that causes extensive yield and quality losses to wheat around the world. Fungicide application and breeding for resistance are among the most important tools to counteract FHB. Biological control is an additional tool that can be used as part of an integrated management of FHB. Bacillus velezensis RC 218, Brevibacillus sp. RC 263 and Streptomyces sp. RC 87B were selected by their potential to control FHB and deoxynivalenol production...
February 25, 2018: Letters in Applied Microbiology
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