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https://www.readbyqxmd.com/read/28813658/ccctc-binding-factor-translates-interleukin-2-and-%C3%AE-ketoglutarate-sensitive-metabolic-changes-in-t%C3%A2-cells-into-context-dependent-gene-programs
#1
Danielle A Chisolm, Daniel Savic, Amanda J Moore, Andre Ballesteros-Tato, Beatriz León, David K Crossman, Cornelis Murre, Richard M Myers, Amy S Weinmann
Despite considerable research connecting cellular metabolism with differentiation decisions, the underlying mechanisms that translate metabolite-sensitive activities into unique gene programs are still unclear. We found that aspects of the interleukin-2 (IL-2)-sensitive effector gene program in CD4(+) and CD8(+) T cells in type 1 conditions (Th1) were regulated by glutamine and alpha-ketoglutarate (αKG)-induced events, in part through changes in DNA and histone methylation states. We further identified a mechanism by which IL-2- and αKG-sensitive metabolic changes regulated the association of CCCTC-binding factor (CTCF) with select genomic sites...
August 15, 2017: Immunity
https://www.readbyqxmd.com/read/28813561/comparison-of-coronary-ct-angiography-spect-pet-and-hybrid-imaging-for-diagnosis-of-ischemic-heart-disease-determined-by-fractional-flow-reserve
#2
Ibrahim Danad, Pieter G Raijmakers, Roel S Driessen, Jonathon Leipsic, Rekha Raju, Chris Naoum, Juhani Knuuti, Maija Mäki, Richard S Underwood, James K Min, Kimberly Elmore, Wynand J Stuijfzand, Niels van Royen, Igor I Tulevski, Aernout G Somsen, Marc C Huisman, Arthur A van Lingen, Martijn W Heymans, Peter M van de Ven, Cornelis van Kuijk, Adriaan A Lammertsma, Albert C van Rossum, Paul Knaapen
Importance: At present, the choice of noninvasive testing for a diagnosis of significant coronary artery disease (CAD) is ambiguous, but nuclear myocardial perfusion imaging with single-photon emission tomography (SPECT) or positron emission tomography (PET) and coronary computed tomography angiography (CCTA) is predominantly used for this purpose. However, to date, prospective head-to-head studies are lacking regarding the diagnostic accuracy of these imaging modalities. Furthermore, the combination of anatomical and functional assessments configuring a hybrid approach may yield improved accuracy...
August 16, 2017: JAMA Cardiology
https://www.readbyqxmd.com/read/28813539/vectorization-by-nanoparticles-decreases-the-overall-toxicity-of-airborne-pollutants
#3
Rodolphe Carpentier, Anne Platel, Helena Maiz-Gregores, Fabrice Nesslany, Didier Betbeder
Atmospheric pollution is mainly composed of volatile pollutants and particulate matter that strongly interact. However, their specific roles in the induction of cellular toxicity, in particular the impact of the vectorization of atmospheric pollutants by ultrafine particles, remains to be fully elucidated. For this purpose, non-toxic poly-lactic co-glycolic acid (PLGA) nanoparticles were synthesized and three pollutants (benzo(a)pyrene, naphthalene and di-ethyl-hexyl-phthalate) were adsorbed on the surface of the nanoparticles in order to evaluate the toxicity (cytotoxicity, genotoxicity and ROS induction) of these complexes to a human airway epithelial cell line...
2017: PloS One
https://www.readbyqxmd.com/read/28813094/fatty-acid-profile-in-vertical-strata-of-elephant-grass-subjected-to-intermittent-stocking
#4
Kamila M Dias, Daniel Schmitt, Giselle R Rodolfo, Francisco C Deschamps, Guilherme N Camargo, Raphael S Pereira, André F Sbrissia
The milk and meat from animals with a pasture-based diet have higher proportions of CLA and C18:3 and lower omega-6:omega-3 ratios than products from animals with diets based on corn silage and concentrate. However, most of the published studies have evaluated fatty acid profiles in temperate climate grasses and the literature with tropical grasses is scarce. Thus, the aim of this study was to evaluate the morphological and fatty acid compositions in the vertical strata of elephant grass (Pennisetum purpureum Schum...
August 14, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28813093/where-is-sugarcane-cropping-expanding-in-the-brazilian-cerrado-and-why-a-case-study
#5
Murilo R DE Arruda, Ken E Giller, Maja Slingerland
Sugarcane growing area in Brazil sharply expanded between 2000 and 2010 due to the increasing world demand for sugar and ethanol. Since this expansion of sugarcane is said to occur in areas covered by degraded pastures, it is likely not threatening the environment or food production. In order to verify this assumption, we investigate at farm and field levels which types of land use sugarcane cropping replaced between 2005 and 2010 and the reasons for farmers shifting or not shifting to sugarcane, as a case study in two counties in the state of Goiás...
August 14, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28812142/tcdd-administered-on-activated-carbon-eliminates-bioavailability-and-subsequent-shifts-to-a-key-murine-gut-commensal
#6
Robert D Stedtfeld, J Brett Sallach, Robert B Crawford, Tiffany M Stedtfeld, Maggie R Williams, Hassan Waseem, Cliff T Johnston, Hui Li, Brian J Teppen, Norbert E Kaminski, Stephen A Boyd, James M Tiedje, Syed A Hashsham
Activated carbon (AC) is an increasingly attractive remediation alternative for the sequestration of dioxins at contaminated sites globally. However, the potential for AC to reduce the bioavailability of dioxins in mammals and the residing gut microbiota has received less attention. This question was partially answered in a recent study examining 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced hallmark toxic responses in mice administered with TCDD sequestered by AC or freely available in corn oil by oral gavage...
August 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28811375/temperature-increase-reduces-global-yields-of-major-crops-in-four-independent-estimates
#7
Chuang Zhao, Bing Liu, Shilong Piao, Xuhui Wang, David B Lobell, Yao Huang, Mengtian Huang, Yitong Yao, Simona Bassu, Philippe Ciais, Jean-Louis Durand, Joshua Elliott, Frank Ewert, Ivan A Janssens, Tao Li, Erda Lin, Qiang Liu, Pierre Martre, Christoph Müller, Shushi Peng, Josep Peñuelas, Alex C Ruane, Daniel Wallach, Tao Wang, Donghai Wu, Zhuo Liu, Yan Zhu, Zaichun Zhu, Senthold Asseng
Wheat, rice, maize, and soybean provide two-thirds of human caloric intake. Assessing the impact of global temperature increase on production of these crops is therefore critical to maintaining global food supply, but different studies have yielded different results. Here, we investigated the impacts of temperature on yields of the four crops by compiling extensive published results from four analytical methods: global grid-based and local point-based models, statistical regressions, and field-warming experiments...
August 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28811335/the-conserved-and-unique-genetic-architecture-of-kernel-size-and-weight-in-maize-and-rice
#8
Jie Liu, Juan Huang, Huan Guo, Liu Lan, Hongze Wang, Yuancheng Xu, Xiaohong Yang, Wenqiang Li, Hao Tong, Yingjie Xiao, Qingchun Pan, Feng Qiao, Mohammad Sharif Raihan, Haijun Liu, Xuehai Zhang, Ning Yang, Xiaqing Wang, Min Deng, Minliang Jin, Lijun Zhao, Xin Luo, Yang Zhou, Xiang Li, Wei Zhan, Nannan Liu, Hong Wang, Gengshen Chen, Qing Li, Jianbing Yan
Maize is a major staple crop. Maize kernel size and weight are important contributors to its yield. Here, we measured kernel length, kernel width, kernel thickness, hundred kernel weight and kernel test weight in 10 recombinant inbred line populations and dissected their genetic architecture using three statistical models. In total, 729 quantitative trait loci (QTLs) were identified, many of which were identified in all three models, including 22 major QTLs that each can explain more than 10% of phenotypic variation...
August 15, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28811114/determination-of-acrylamide-levels-in-potato-crisps-and-other-snacks-and-exposure-risk-assessment-through-a-margin-of-exposure-approach
#9
Francesco Esposito, Antonio Nardone, Evelina Fasano, Maria Triassi, Teresa Cirillo
Potato crisps, corn-based extruded snacks and other savoury snacks are very popular products especially among younger generations. These products could be a potential source of acrylamide (AA), a toxic compound which could develop during frying and baking processes. The purpose of this study was the assessment of the dietary intake to AA across six groups of consumers divided according to age through the consumption of potato crisps and other snacks, in order to eventually evaluate the margin of exposure (MOE) related to neurotoxic and carcinogenic critical endpoints...
August 12, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28811113/acrylamide-forming-potential-of-cereals-legumes-and-roots-and-tubers-analyzed-by-uplc-uv
#10
Y J H Galani, N J Patel, J G Talati
For directing scientists, consumers, industry and stakeholders on mitigation strategies, there is a need to understand the acrylamide-forming potential of important Indian foods. Flour obtained from total 16 varieties of 9 Indian cereals, legumes and roots and tubers was heated at 160 °C for 20 min, acrylamide was extracted and quantified by UPLC-UV. Acrylamide level was above the European Commission indicative value in potato- and cereal-based food products, it ranged from 3436.13 to 5562.56 μg/kg in roots and tubers (potato and sweet potato)...
August 12, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28810832/genome-wide-analysis-of-basic-helix-loop-helix-bhlh-transcription-factors-in-brachypodium-distachyon
#11
Xin Niu, Yuxiang Guan, Shoukun Chen, Haifeng Li
BACKGROUND: As a superfamily of transcription factors (TFs), the basic helix-loop-helix (bHLH) proteins have been characterized functionally in many plants with a vital role in the regulation of diverse biological processes including growth, development, response to various stresses, and so on. However, no systemic analysis of the bHLH TFs has been reported in Brachypodium distachyon, an emerging model plant in Poaceae. RESULTS: A total of 146 bHLH TFs were identified in the Brachypodium distachyon genome and classified into 24 subfamilies...
August 15, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28809013/a-step-by-step-guide-to-assemble-a-reptilian-genome
#12
Asier Ullate-Agote, Yingguang Frank Chan, Athanasia C Tzika
Multiple technologies and software are now available facilitating the de novo sequencing and assembly of any vertebrate genome. Yet the quality of most available sequenced genomes is substantially poorer than that of the golden standard in the field: the human genome. Here, we present a step-by-step protocol for the successful sequencing and assembly of a high-quality snake genome that can be applied to any other reptilian or avian species. We combine the great sequencing depth and accuracy of short reads with the use of different insert size libraries for extended scaffolding followed by optical mapping...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28808417/down-regulation-of-aminopeptidase-n-and-abc-transporter-subfamily-g-transcripts-in-cry1ab-and-cry1ac-resistant-asian-corn-borer-ostrinia-furnacalis-lepidoptera-crambidae
#13
Tiantao Zhang, Brad S Coates, Yueqin Wang, Yidong Wang, Shuxiong Bai, Zhenying Wang, Kanglai He
The Asian corn borer (ACB), Ostrinia furnacalis (Lepidoptera: Crambidae), is a highly destructive pest of cultivated maize throughout East Asia. Bacillus thuringiensis (Bt) crystalline protein (Cry) toxins cause mortality by a mechanism involving pore formation or signal transduction following toxin binding to receptors along the midgut lumen of susceptible insects, but this mechanism and mutations therein that lead to resistance are not fully understood. In the current study, quantitative comparisons were made among midgut expressed transcripts from O...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28808096/strategies-and-tools-to-improve-crop-productivity-by-targeting-photosynthesis
#14
Michael L Nuccio, Laura Potter, Suzy M Stiegelmeyer, Joseph Curley, Jonathan Cohn, Peter E Wittich, Xiaoping Tan, Jimena Davis, Junjian Ni, Jon Trullinger, Rick Hall, Nicholas J Bate
Crop productivity needs to substantially increase to meet global food and feed demand for a rapidly growing world population. Agricultural technology developers are pursuing a variety of approaches based on both traditional technologies such as genetic improvement, pest control and mechanization as well as new technologies such as genomics, gene manipulation and environmental modelling to develop crops that are capable of meeting growing demand. Photosynthesis is a key biochemical process that, many suggest, is not yet optimized for industrial agriculture or the modern global environment...
September 26, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28807837/characterization-of-two-odorant-binding-proteins-in-spodoptera-exigua-reveals-functional-conservation-and-difference
#15
Nai-Yong Liu, Jia-Yao Zhu, Ting Zhang, Shuang-Lin Dong
As the first biochemical step of olfactory reception and recognition, odorant binding proteins (OBPs) have been demonstrated to be essential. Considering functional diversities of OBPs within a single species, we here extended the characterization of two other OBPs from Spodoptera exigua, belonging to insect Classic OBPs. With a combination of transcriptome and Rapid Amplification of cDNA End (RACE) approaches, two OBP genes in S. exigua were first identified, namely SexiOBP1 and OBP7. Expression pattern analysis revealed that both of them exhibited a distinct expression, where OBP1 was broadly and highly expressed in several tissues including antennae of adults whereas OBP7 was abundant only in the antennae of both sexes, strongly indicative of olfactory roles...
August 11, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28807084/linseed-plus-nitrate-in-the-diet-for-fattening-bulls-effects-on-methane-emission-animal-health-and-residues-in-offal
#16
M Doreau, M Arbre, M Popova, Y Rochette, C Martin
The combination of linseed and nitrate is known to decrease enteric methane emission in dairy cows but few studies have been carried out in fattening cattle for animal liveweight gain, enteric methane emission, animal health and presence of residues in beef products. To address this gap, 16 young bulls received a control (C) diet between weaning at 9 months and 14 months, then were split into two groups of eight balanced on feed intake, BW gain and methane emission to receive either the C diet or a diet moderately supplemented with extruded linseed and calcium nitrate (LN) for 2 months before being slaughtered...
August 15, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28806927/mapping-of-a-major-qtl-for-salt-tolerance-of-mature-field-grown-maize-plants-based-on-snp-markers
#17
Meijie Luo, Yanxin Zhao, Ruyang Zhang, Jinfeng Xing, Minxiao Duan, Jingna Li, Naishun Wang, Wenguang Wang, Shasha Zhang, Zhihui Chen, Huasheng Zhang, Zi Shi, Wei Song, Jiuran Zhao
BACKGROUND: Salt stress significantly restricts plant growth and production. Maize is an important food and economic crop but is also a salt sensitive crop. Identification of the genetic architecture controlling salt tolerance facilitates breeders to select salt tolerant lines. However, the critical quantitative trait loci (QTLs) responsible for the salt tolerance of field-grown maize plants are still unknown. RESULTS: To map the main genetic factors contributing to salt tolerance in mature maize, a double haploid population (240 individuals) and 1317 single nucleotide polymorphism (SNP) markers were employed to produce a genetic linkage map covering 1462...
August 15, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28806524/assessment-of-effects-of-the-long-term-exposure-of-agricultural-crops-to-carbon-nanotubes
#18
Mohamed Hassen Lahiani, Zeid A Nima, Hector Villagarcia, Alexandru S Biris, Mariya Khodakovskaya
Carbon-based nanomaterials (CBNs) were described as nanomaterials possessing abilities of plant growth regulators. Here, we investigated the effects of long-term exposure of multi-walled carbon nanotubes (MWCNTs) on the growth of three important crops (barley, soybean, corn). The cultivation of all tested species was carried in hydroponics supplemented with 50µg/ml of MWCNTs. After 20 weeks of continuous exposure to the nanomaterials, no significant toxic signs on plant development were observed. Several positive phenotypical changes were recorded in addition to the enhancement of photosynthesis in MWCNTs-exposed crops...
August 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28805922/use-of-a-complete-starter-feed-in-grain-adaptation-programs-for-feedlot-cattle
#19
C J Schneider, B L Nuttelman, A L Shreck, D B Burken, W A Griffin, J L Gramkow, R A Stock, T J Klopfenstein, G E Erickson
Four experiments evaluated the use of a complete starter feed (RAMP; Cargill Corn Milling, Blair, NE) for grain adaptation. In Exp. 1, 229 yearling steers (397 ± 28.4 kg BW) were used to compare a traditional adaptation program (CON) with adapting cattle with RAMP in either a 1- (RAMP-1RS) or 2- (RAMP-2RS) ration system. From d 23 to slaughter, cattle were fed a common finishing diet. In Exp. 2, 390 yearling steers (341 ± 14 kg BW) were used to compare accelerated grain adaptation programs with RAMP with 2 control treatments where RAMP was blended with a finishing diet containing either 25 (CON25) or 47...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28805905/standardized-ileal-digestible-amino-acids-and-digestible-energy-contents-in-high-protein-distiller-s-dried-grains-with-solubles-fed-to-growing-pigs
#20
Y Rho, C Zhu, E Kiarie, C F M de Lange
Chemical composition and, therefore, potential nutritive value of corn distiller's dried grains with solubles (DDGS) is constantly evolving as the ethanol industry innovates processing techniques. We determined standardized ileal digestibility (SID) of AA (Exp. 1) and DE (Exp. 2) contents in high-protein (HP; >40% on a DM basis) DDGS samples. Two HP DDGS (HP-A and HP-B) samples and 1 conventional DDGS (CON) sample were selected for this study. Three semipurified corn starch-based diets (minimum 18% CP, as-fed basis) were formulated with DDGS as the sole source of AA...
August 2017: Journal of Animal Science
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