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https://www.readbyqxmd.com/read/28317353/-clinical-application-on-restoration-of-dentition-defects-using-tooth-alveolar-bone-grafts
#1
Chen Zhifang, Zhang Wei
OBJECTIVE: To evaluate the clinical effectiveness of tooth-alveolar bone grafts in repairing dentition defects. METHODS: Forty-five patients with dentition defect were divided into two groups according to different operation methods. In group A (n=24), the tooth-alveolar bone grafts of the transplanted tooth and its surrounding alveolar bone were cut using modified surgical orthodontics and then embedded into the designated planting hole. In group B (n=21), the teeth were extracted through conventional tooth transplantation and then embedded into the prepared planting holes...
August 1, 2016: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28300208/practical-whole-tooth-restoration-utilizing-autologous-bioengineered-tooth-germ-transplantation-in-a-postnatal-canine-model
#2
Mitsuaki Ono, Masamitsu Oshima, Miho Ogawa, Wataru Sonoyama, Emilio Satoshi Hara, Yasutaka Oida, Shigehiko Shinkawa, Ryu Nakajima, Atsushi Mine, Satoru Hayano, Satoshi Fukumoto, Shohei Kasugai, Akira Yamaguchi, Takashi Tsuji, Takuo Kuboki
Whole-organ regeneration has great potential for the replacement of dysfunctional organs through the reconstruction of a fully functional bioengineered organ using three-dimensional cell manipulation in vitro. Recently, many basic studies of whole-tooth replacement using three-dimensional cell manipulation have been conducted in a mouse model. Further evidence of the practical application to human medicine is required to demonstrate tooth restoration by reconstructing bioengineered tooth germ using a postnatal large-animal model...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28286291/human-%C3%AE-defensin-2-and-interleukin-1%C3%AE-expression-in-response-to-porphyromonas-gingivalis-challenge-in-mice-transplanted-with-periodontitic-human-gingiva
#3
Tomoko Shimizu, Masahiro To, Yohei Kamata, Juri Saruta, Takenori Sato, Shinya Fuchida, Nobushiro Hamada, Keiichi Tsukinoki
Inter-individual variability in the host response contributes significantly to expression of periodontal disease. Thus, research into the human host response is considered important in the analysis of periodontal disease. Human β-defensin-2 (hBD-2) is typically produced by epithelial tissues after stimulation with microorganisms and inflammatory mediators, and it contributes to the initial defense in the innate immune response. However, hBD-2 expression in response to infection has not been investigated in human gingival tissue with periodontitis...
March 9, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28224037/tooth-extraction-in-a-patient-after-autologous-skeletal-myoblast-sheet-transplantation-for-severe-dilated-cardiomyopathy
#4
Kohei Okuyama, Yuki Sakamoto, Tomofumi Naruse, Souichi Yanamoto, Masahiro Umeda
The authors describe the first case of tooth extraction in a patient treated with autologous skeletal myocardial sheet transplantation for severe dilated cardiomyopathy. This condition is associated with significant morbidity and mortality that may have identifiable causes or may be idiopathic or inherited. In addition to local disinfection, antimicrobial prophylaxis and continuous anticoagulant therapy during the perioperative period of tooth extraction were needed to prevent infective endocarditis and ensure the success of regenerative therapy of the heart...
March 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/28217369/regenerative-medicine-using-dental-pulp-stem-cells-for-liver-diseases
#5
EDITORIAL
Shogo Ohkoshi, Hajime Hara, Haruka Hirono, Kazuhiko Watanabe, Katsuhiko Hasegawa
Acute liver failure is a refractory disease and its prognosis, if not treated using liver transplantation, is extremely poor. It is a good candidate for regenerative medicine, where stem cell-based therapies play a central role. Mesenchymal stem cells (MSCs) are known to differentiate into multiple cell lineages including hepatocytes. Autologous cell transplant without any foreign gene induction is feasible using MSCs, thereby avoiding possible risks of tumorigenesis and immune rejection. Dental pulp also contains an MSC population that differentiates into hepatocytes...
February 6, 2017: World Journal of Gastrointestinal Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28185382/autogenous-bone-block-grafting-provides-facial-implant-tissue-stability-long-term
#6
Fernando Verdugo, Agurne Uribarri, Antonio D'Addona
BACKGROUND: Mucosal recession (MR) and bone loss can compromise anterior implant esthetics. PURPOSE: To evaluate tissue stability and clinical outcomes of anterior implants augmented with autogenous block transplants long-term. MATERIALS AND METHODS: This prospective cross-sectional clinical study analyzed facial tissue recession of anterior implants augmented with autogenous bone blocks and compared them to adjacent teeth in forty patients 52 months post-augmentation...
February 10, 2017: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/28179939/autogenous-transplantation-for-replacing-a-hopeless-tooth
#7
Mehrsa Zakershahrak, Amirabbas Moshari, Mehdi Vatanpour, Zohreh Khalilak, Afsoon Jalali Ara
Autogenous tooth transplantation (ATT) is a simple and reasonable choice for replacing the missing teeth when a proper donor tooth is available. This report presents a case of successful ATT of a maxillary right third molar for replacement of mandibular right second molar with a concomitant endodontic-periodontal disease. The mandibular second molar was believed to be hopeless due to a severe damage to coronal tooth structure, inappropriate root canal treatment and apical radiolucency. After extraction of mandibular second molar and maxillary third molar (the donor), the tooth was re-implanted into the extracted socket of second molar site...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28177531/recruitment-of-bone-marrow-derived-cells-to-the-periodontal-ligament-via-the-stromal-cell-derived-factor-1-c-x-c-chemokine-receptor-type-4-axis
#8
M Kaku, M Kitami, J M Rosales Rocabado, T Ida, Y Akiba, K Uoshima
BACKGROUND/OBJECTIVES: The periodontal ligament (PDL) is a non-mineralized connective tissue that exists between the alveolar bone and root surface cementum and plays important roles in tooth function. The PDL harbors a remarkable reserve of multipotent stem cells, which maintain various types of cells. However, the sources of these stem cells, other than their developmental origin, are not well understood. MATERIAL AND METHODS: To elucidate the recruitment of bone marrow (BM)-derived stem cells in the PDL, green fluorescent protein (GFP)-expressing BM-derived cells were transplanted into the femoral BM of immunodeficient rats, and the distribution and expression of stem cell markers in the PDL were analyzed in vivo...
February 8, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28168900/a-translational-medicine-approach-to-tooth-transplantation
#9
Giovanpaolo Pini Prato, Giliana Zuccati, Carlo Clauser
No abstract text is available yet for this article.
February 7, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28106505/pulp-vascularization-during-tooth-development-regeneration-and-therapy
#10
C Rombouts, T Giraud, C Jeanneau, I About
The pulp is a highly vascularized tissue situated in an inextensible environment surrounded by rigid dentin walls, with the apical foramina being the only access. The pulp vascular system is not only responsible for nutrient supply and waste removal but also contributes actively to the pulp inflammatory response and subsequent regeneration. This review discusses the underlying mechanisms of pulp vascularization during tooth development, regeneration, and therapeutic procedures, such as tissue engineering and tooth transplantation...
February 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28061389/in-vitro-proliferation-of-periodontal-ligament-like-tissue-on-extracted-teeth
#11
T Iwata, C Mino, T Kawata
OBJECTIVE: Transplantation of autologous teeth is a routine component of orthodontic treatment. The aim of this study was to develop a method for the regeneration of damaged periodontal ligament (PDL) on extracted teeth using a three-dimensional culture system. DESIGN: We used the maxillary first premolars or third molars extracted from patients for orthodontic treatment. The extracted teeth were stained with toluidine blue to measure the residual PDL area. After confirming damage of the periodontal tissue on the root surface of the extracted teeth, we tried to regenerate the periodontal tissue...
November 30, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28057509/promoting-bioengineered-tooth-innervation-using-nanostructured-and-hybrid-scaffolds
#12
S Kuchler-Bopp, A Larrea, L Petry, Y Idoux-Gillet, V Sebastian, A Ferrandon, P Schwinté, M Arruebo, N Benkirane-Jessel
The innervation of teeth mediated by axons originating from the trigeminal ganglia is essential for their function and protection. Immunosuppressive therapy using Cyclosporine A (CsA) was found to accelerate the innervation of transplanted tissues and particularly that of bioengineered teeth. To avoid the CsA side effects, we report in this study the preparation of CsA loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles, their embedding on polycaprolactone (PCL)-based scaffolds and their possible use as templates for the innervation of bioengineered teeth...
January 3, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28053317/jawbone-microenvironment-promotes-periodontium-regeneration-by-regulating-the-function-of-periodontal-ligament-stem-cells
#13
Bin Zhu, Wenjia Liu, Yihan Liu, Xicong Zhao, Hao Zhang, Zhuojing Luo, Yan Jin
During tooth development, the jawbone interacts with dental germ and provides the development microenvironment. Jawbone-derived mesenchymal stem cells (JBMSCs) maintain this microenvironment for root and periodontium development. However, the effect of the jawbone microenvironment on periodontium tissue regeneration is largely elusive. Our previous study showed that cell aggregates (CAs) of bone marrow mesenchymal stem cells promoted periodontium regeneration on the treated dentin scaffold. Here, we found that JBMSCs enhanced not only the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) but also their adhesion to titanium (Ti) material surface...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28027709/conditioned-medium-from-periodontal-ligament-stem-cells-enhances-periodontal-regeneration
#14
Mizuki Nagata, Kengo Iwasaki, Keiko Akazawa, Motohiro Komaki, Naoki Yokoyama, Yuichi Izumi, Ikuo Morita
Periodontal disease is one of the most common infectious diseases in adults and is characterized by the destruction of tooth-supporting tissues. Mesenchymal stem cells (MSCs) comprise the mesoderm-originating stem cell population, which has been studied and used for cell therapy. However, because of the lower rate of cell survival after MSC transplantation in various disease models, paracrine functions of MSCs have been receiving increased attention as a regenerative mechanism. The aim of this study was to investigate the regenerative potential of transplanted conditioned medium (CM) obtained from cultured periodontal ligament stem cells (PDLSCs), the adult stem cell population in tooth-supporting tissues, using a rat periodontal defect model...
January 27, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27974406/thrombotic-microangiopathy-in-inverted-formin-2-mediated-renal-disease
#15
Rachel C Challis, Troels Ring, Yaobo Xu, Edwin K S Wong, Oliver Flossmann, Ian S D Roberts, Saeed Ahmed, Michael Wetherall, Giedrius Salkus, Vicky Brocklebank, Julian Fester, Lisa Strain, Valerie Wilson, Katrina M Wood, Kevin J Marchbank, Mauro Santibanez-Koref, Timothy H J Goodship, David Kavanagh
The demonstration of impaired C regulation in the thrombotic microangiopathy (TMA) atypical hemolytic uremic syndrome (aHUS) resulted in the successful introduction of the C inhibitor eculizumab into clinical practice. C abnormalities account for approximately 50% of aHUS cases; however, mutations in the non-C gene diacylglycerol kinase-ε have been described recently in individuals not responsive to eculizumab. We report here a family in which the proposita presented with aHUS but did not respond to eculizumab...
December 14, 2016: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/27973697/enhanced-regeneration-potential-of-mobilized-dental-pulp-stem-cells-from-immature-teeth
#16
Hidenori Nakayama, Koichiro Iohara, Yuki Hayashi, Yuta Okuwa, Kurita Kenichi, Misako Nakashima
OBJECTIVES: We have previously demonstrated that dental pulp stem cell (DPSCs) isolated from mature teeth by granulocyte-colony stimulating factor (G-CSF)-induced mobilization method can enhance angiogenesis/vasculogenesis and improve pulp regeneration when compared with colony-derived DPSCs. However, the efficacy of this method in immature teeth with root-formative stage has never been investigated. Therefore, the aim of this study is to examine the stemness, biological characteristics and regeneration potential in mobilized-DPSCs compared with colony-derived DPSCs from immature teeth...
December 14, 2016: Oral Diseases
https://www.readbyqxmd.com/read/27925870/hydrogen-sulfide-enhances-pancreatic-%C3%AE-cell-differentiation-from-human-tooth-under-normal-and-glucotoxic-conditions
#17
Ioan Valentin Matei, Hisataka Ii, Ken Yaegaki
AIM: Glucotoxicity obstructs pancreatic differentiation from adult stem cells. The aim was to develop a novel protocol for differentiation of dental pulp stem cells (DPSCs) into pancreatic β cells and determine the effect of H2S on glucotoxicity. MATERIALS & METHODS: DPSCs were differentiated with media containing 5.5 or 25.0 mM glucose, exposed to 1 ng/ml H2S. Glucotoxicity, expression of β-cell markers, INS, PDX1 and GLUT2, and PI3K/AKT pathway were assessed...
December 7, 2016: Regenerative Medicine
https://www.readbyqxmd.com/read/27858309/dental-care-approach-in-patients-with-osteopetrosis
#18
V Detailleur, G Vansteenkiste, M Renard, A Verdonck
AIM: To describe dental and dentofacial characteristics observed in patients diagnosed with osteopetrosis and to advise a dental care approach in these patients. METHODS: Four patients were clinically diagnosed with osteopetrosis, characterised by increased bone density, bone marrow failure, blindness and deafness due to compression of cranial nerves. All patients were dentally screened at different ages (2.5-31 years) and three of them were treated with a haematopoietic stem cell transplantation (HSCT) at the age of 6 months, 1 and 3...
December 2016: European Archives of Paediatric Dentistry: Official Journal of the European Academy of Paediatric Dentistry
https://www.readbyqxmd.com/read/27776880/guided-autotransplantation-of-teeth-a-novel-method-using-virtually-planned-3-dimensional-templates
#19
Georg D Strbac, Albrecht Schnappauf, Katharina Giannis, Michael H Bertl, Andreas Moritz, Christian Ulm
INTRODUCTION: The aim of this study was to introduce an innovative method for autotransplantation of teeth using 3-dimensional (3D) surgical templates for guided osteotomy preparation and donor tooth placement. METHODS: This report describes autotransplantation of immature premolars as treatment of an 11-year-old boy having suffered severe trauma with avulsion of permanent maxillary incisors. This approach uses modified methods from guided implant surgery by superimposition of Digital Imaging and Communications in Medicine files and 3D data sets of the jaws in order to predesign 3D printed templates with the aid of a fully digital workflow...
December 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27747624/evaluation-of-implant-materials-as-cell-carriers-for-dental-stem-cells-under-in-vitro-conditions
#20
Martin Gosau, Sandra Viale-Bouroncle, Hannah Eickhoff, Esthera Prateeptongkum, Anja Reck, W Götz, Christoph Klingelhöffer, Steffen Müller, Christian Morsczeck
BACKGROUND: Dental stem cells in combination with implant materials may become an alternative to autologous bone transplants. For tissue engineering different types of soft and rigid implant materials are available, but little is known about the viability and the osteogenic differentiation of dental stem cells on these different types of materials. According to previous studies we proposed that rigid bone substitute materials are superior to soft materials for dental tissue engineering...
December 2015: International Journal of Implant Dentistry
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