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https://www.readbyqxmd.com/read/29055227/generation-and-characterization-of-human-ipsc-lines-sani001-a-and-sani002-a-from-mobilized-peripheral-blood-derived-megakaryoblasts
#1
Marten Hansen, Eszter Varga, Tatjana Wüst, Clemens Mellink, Anne-Marie van der Kevie-Kersemaekers, Marieke von Lindern, Emile van den Akker
Mobilized peripheral blood (MPB) CD34+ cells were differentiated to CD34(+)/CD41(+) megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population which was reprogrammed with a hOKSM self-silencing polycistronic lentiviral vector. Resulting iPSC showed normal karyotype and expression of pluripotency associated markers and in vitro spontaneous differentiation towards the 3 germ layers confirmed pluripotency of iPSC lines. Besides normal iPSC applications, these lines can be used as a control line for other megakaryoid origin iPSC and could be applied for epigenetic based research...
October 12, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29054803/comparison-of-short-term-effects-of-mobilization-with-movement-and-kinesiotaping-on-pain-function-and-balance-in-patellofemoral-pain
#2
Serdar Demirci, Gizem Irem Kinikli, Michael J Callaghan, Volga Bayrakci Tunay
OBJECTIVE: The aim of this study was to compare the short-term effects of Mobilization with movement (MWM) and Kinesiotaping (KT) on patients with patellofemoral pain (PFP) respect to pain, function and balance. METHODS: Thirty-five female patients diagnosed with unilateral PFP were assigned into 2 groups. The first group (n = 18) received two techniques of MWM intervention (Straight Leg-Raise with Traction and Tibial Gliding) while KT was applied to the other group (n = 17)...
October 17, 2017: Acta Orthopaedica et Traumatologica Turcica
https://www.readbyqxmd.com/read/29053517/total-palatal-mobilization-and-multilamellar-suturing-technique-improves-outcome-for-palatal-fistula-repair
#3
Ahmed Elsherbiny, John H Grant
BACKGROUNDS: The success rate of the surgical repair of palatal fistula after palatoplasty is often unsatisfactory. This study is a review of 15 years of single surgeon's experience with the evolution of a reliable surgical technique with high success rate. METHODS: This is a retrospective chart review of consecutive cleft cases undergoing repair of palatal fistula from 2000 to 2015. The study included 37 consecutive fistula repair cases with wide elevation and mobilization of the palatal tissues and nasal and oral layer repair...
October 19, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/29053491/safety-and-feasibility-of-early-physical-therapy-for-patients-on-extracorporeal-membrane-oxygenator-university-of-maryland-medical-center-experience
#4
Chris L Wells, Jenny Forrester, Joshua Vogel, Raymond Rector, Ali Tabatabai, Daniel Herr
OBJECTIVES: To examine the feasibility and safety of mobilizing patients while on extracorporeal membranous oxygenation support. DESIGN: Retrospective cohort study. SETTING: Medical and Surgical ICUs in a large tertiary care hospital in the United States. PATIENTS: Adults supported on extracorporeal membranous oxygenation from January 2014 to December 2015. MEASUREMENTS AND MAIN RESULTS: We reviewed the medical records from physical therapy, perfusion, and intensivists to obtain the number and type of physical therapy interventions and discharge status; extracorporeal membranous oxygenation type and description of support, cannulation sites; and risk management details of adverse effects, if any...
October 19, 2017: Critical Care Medicine
https://www.readbyqxmd.com/read/29052742/-triple-arthrodesis-for-correction-of-cavovarus-deformity
#5
REVIEW
A Barg, R Ruiz, B Hintermann
OBJECTIVE: The aim is to correct the underlying cavovarus deformity and to achieve a pain-free and stable hindfoot. INDICATIONS: Rigid neurologic, posttraumatic, congenital, and idiopathic cavovarus deformities. CONTRAINDICATIONS: General surgical or anesthesiological risks, infections, critical soft tissue conditions, neurovascular impairment of the lower extremity, noncompliance, patients with severely reduced bone quality, insulin-dependent diabetes mellitus, smoking...
October 20, 2017: Operative Orthopädie und Traumatologie
https://www.readbyqxmd.com/read/29051881/fracture-management-in-pyknodysostosis-a-rare-case-report
#6
G Subramanya Gandhi, V Vijayanarasimhan, Lionel John, S Kailash, E Sathish Balaji
INTRODUCTION: Pyknodysostosis is congenital osteosclerotic skeletal dysplasia of a rare entity. It is an autosomal recessive disease which presents with short stature and generalized osteosclerosis of skeleton as result of decreased bone turnover. Here, the authors report a case of pyknodysostosis who presented to our emergency room with a pathological fracture in the shaft of the femur for which operative intervention was carried out. CASE REPORT: A 30-year-old female presented to our emergency room with a history of trivial injury to right thigh region and was diagnosed as fracture in her right femur shaft region...
May 2017: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/29051869/case-of-acute-concomitant-rupture-of-anterior-cruciate-ligament-and-patellar-tendon-of-knee-surgical-decision-making-and-outcome
#7
Jithin Orville Lobo, Joe Joseph Cherian, Arnav Sahu
INTRODUCTION: Acute concomitant rupture of both anterior cruciate ligament (ACL) and patellar tendon is a rare injury. The diagnosis of either of the injuries may be missed on clinical examination in the emergency room. A high index of suspicion is necessary when active extension is absent, and necessary investigations like ultrasound or magnetic resonance imaging (MRI) should be done to confirm the diagnosis. There are no fixed treatment protocols in these injuries. The options include immediate repair of patellar tendon with either simultaneous or delayed reconstruction of ACL...
May 2017: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/29051450/differential-effects-of-low-molecular-weight-organic-acids-on-the-mobilization-of-soil-borne-arsenic-and-trace-metals
#8
Obinna Elijah Nworie, Junhao Qin, Chuxia Lin
A batch experiment was conducted to examine the effects of six low-molecular-weight organic acids on the mobilization of arsenic and trace metals from a range of contaminated soils. The results showed that the organic acids behaved differently when reacting with soil-borne As and trace metals. Oxalic acid and acetic acid had the strongest and weakest capacity to mobilize the investigated elements, respectively. The solubilisation of iron oxides by the organic acids appears to play a critical role in mobilizing other trace metals and As...
August 21, 2017: Toxics
https://www.readbyqxmd.com/read/29051319/ccl26-participates-in-the-prl-3-induced-promotion-of-colorectal-cancer-invasion-by-stimulating-tumor-associated-macrophage-infiltration
#9
Qiusheng Lan, Wei Lai, Yujie Zeng, Lu Liu, Shoufeng Li, Shaowen Jin, Yang Zhang, Xingxi Luo, Heyang Xu, Xiangan Lin, Zhonghua Chu
Both phosphatase of regenerating liver-3 (PRL-3) and tumor-associated macrophages (TAMs) influence cancer progression. Whether PRL-3 plays a critical role in colorectal cancer (CRC) invasion and metastasis by inducing TAM infiltration remains unclear. In the present study, we investigated the effects of chemokine ligand 26 (CCL26) on TAM infiltration and CRC invasion and the underlying mechanism in CRC cells by overexpressing or silencing PRL-3. We found that PRL-3 up-regulated CCL26 expression correlatively and participated in cell migration, according to the results of gene ontology analysis...
October 19, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29051228/thermal-strategies-of-king-penguins-during-prolonged-fasting-in-water
#10
Agnès Lewden, Manfred R Enstipp, Batshéva Bonnet, Caroline Bost, Jean-Yves Georges, Yves Handrich
Most animals experience periods of unfavourable conditions, challenging their daily energy balance. During breeding, king penguins fast voluntarily for up to 1.5 months in the colony, after which they replenish their energy stores at sea. However, at sea, birds might encounter periods of low foraging profitability, forcing them to draw from previously stored energy (e.g. subcutaneous fat). Accessing peripheral fat stores requires perfusion, increasing heat loss and thermoregulatory costs. Hence, how these birds balance the conflicting demands of nutritional needs and thermoregulation is unclear...
October 19, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29051196/mechanisms-of-phosphorus-acquisition-and-lipid-class-remodelling-under-p-limitation-in-a-marine-microalga
#11
Alice Mühlroth, Per Winge, Aimen El Assimi, Juliette Jouhet, Eric Marechal, Martin F Hohmann-Marriott, Olav Vadstein, Atle M Bones
Molecular mechanisms of phosphorus (P) limitation are of great interest for understanding algal production in aquatic ecosystems. Previous studies point to P limitation induced changes in lipid composition. As in microalgae the molecular mechanisms of this specific P-stress adaptation remain unresolved, we reveal a detailed phospholipid-recycling scheme in Nannochloropsis oceanica and described important P acquisition genes based on highly corresponding transcriptome and lipidome data. Initial responses to P limitation showed increased expression of genes involved in P uptake and an expansion of P substrate spectrum based on purple acid phosphatases...
October 19, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29051137/alzheimer-s-disease-in-social-media-content-analysis-of-youtube-videos
#12
Weizhou Tang, Kate Olscamp, Seul Ki Choi, Daniela B Friedman
BACKGROUND: Approximately 5.5 million Americans are living with Alzheimer's disease (AD) in 2017. YouTube is a popular platform for disseminating health information; however, little is known about messages specifically regarding AD that are being communicated through YouTube. OBJECTIVE: This study aims to examine video characteristics, content, speaker characteristics, and mobilizing information (cues to action) of YouTube videos focused on AD. METHODS: Videos uploaded to YouTube from 2013 to 2015 were searched with the term "Alzheimer's disease" on April 30th, 2016...
October 19, 2017: Interactive Journal of Medical Research
https://www.readbyqxmd.com/read/29050687/comparison-of-adverse-events-and-postoperative-mobilization-following-knee-extensor-mechanism-rupture-repair-a-systematic-review-and-network-meta-analysis
#13
Joseph Serino, Amin Mohamadi, Sebastian Orman, Brian McCormick, Philip Hanna, Michael J Weaver, Mitchel B Harris, Ara Nazarian, Arvind von Keudell
BACKGROUND: Extensor mechanism rupture (EMR) of the knee is a rare but potentially debilitating injury that often occurs due to trauma. While a wide variety of surgical treatments have been reported, there is currently no consensus on the most successful treatment method. The timing of post-operative joint mobilization is also critical for successful recovery after EMR repair. Despite the traditional method of complete immobilization for 6 weeks, there is an increasing trend towards early post-operative knee mobilization...
October 16, 2017: Injury
https://www.readbyqxmd.com/read/29050655/healthy-by-design-using-a-gender-focus-to-influence-complete-streets-policy
#14
April Ennis Keippel, Melissa A Henderson, Amanda L Golbeck, TommiLee Gallup, Diane K Duin, Stephen Hayes, Stephanie Alexander, Elizabeth L Ciemins
BACKGROUND: Public health leaders in Yellowstone County, Montana, formed an alliance to address community-wide issues. One such issue is Complete Streets, with its vision of safe streets for all. This case study focuses on development and adoption of a Complete Streets policy. It examines how a community coalition, Healthy By Design, infused a gender focus into the policymaking process. METHODS: An incremental and nonlinear policymaking process was aided by a focus on gender and health equity...
October 17, 2017: Women's Health Issues: Official Publication of the Jacobs Institute of Women's Health
https://www.readbyqxmd.com/read/29050653/policy-impact-of-research-findings-on-the-association-of-diaper-need-and-mental-health
#15
Lori R Wallace, Alison M Weir, Megan V Smith
BACKGROUND: Maternal mental illness constitutes one of most significant public health problems facing women of reproductive age and their children. Women who mother in poverty experience additional stressors, pointing to the need for innovative public health promotion efforts to reduce mental illness. Simple innovations, like making diapers more available and affordable, may represent an effective method of addressing this problem. This paper describes the research findings on diaper need reported by the New Haven Mental Health Outreach for Mothers Partnership and the process of translating these findings into broader policy and advocacy efforts...
October 17, 2017: Women's Health Issues: Official Publication of the Jacobs Institute of Women's Health
https://www.readbyqxmd.com/read/29049338/growing-plants-on-oily-nutrient-poor-soil-using-a-native-symbiotic-fungus
#16
Timothy S Repas, D Michael Gillis, Zakia Boubakir, Xiaohui Bao, Gary J Samuels, Susan G W Kaminskyj
The roots of land plants associate with microbes, including fungal symbionts that can confer abiotic stress tolerance. Bitumen extraction following oil-sand surface mining in the Athabasca region of Alberta, Canada removes plant nutrients but leaves a petrochemical residue, making the coarse tailings (CT) hostile to both plants and microbes. We isolated an endophyte strain of the Ascomycete Trichoderma harzianum we call TSTh20-1 (hereafter, TSTh) from a dandelion that was naturally growing on CT. TSTh colonization allowed tomato, wheat, and remediation seed mixtures to germinate and their seedlings to flourish on CT without the use of fertilizer...
2017: PloS One
https://www.readbyqxmd.com/read/29049279/knowledge-attitudes-and-practices-related-to-ebola-virus-disease-at-the-end-of-a-national-epidemic-guinea-august-2015
#17
Mohamed F Jalloh, Susan J Robinson, Jamaica Corker, Wenshu Li, Kathleen Irwin, Alpha M Barry, Paulyne Ngalame Ntuba, Alpha A Diallo, Mohammad B Jalloh, James Nyuma, Musa Sellu, Amanda VanSteelandt, Megan Ramsden, LaRee Tracy, Pratima L Raghunathan, John T Redd, Lise Martel, Barbara Marston, Rebecca Bunnell
Health communication and social mobilization efforts to improve the public's knowledge, attitudes, and practices (KAP) regarding Ebola virus disease (Ebola) were important in controlling the 2014-2016 Ebola epidemic in Guinea (1), which resulted in 3,814 reported Ebola cases and 2,544 deaths.* Most Ebola cases in Guinea resulted from the washing and touching of persons and corpses infected with Ebola without adequate infection control precautions at home, at funerals, and in health facilities (2,3). As the 18-month epidemic waned in August 2015, Ebola KAP were assessed in a survey among residents of Guinea recruited through multistage cluster sampling procedures in the nation's eight administrative regions (Boké, Conakry, Faranah, Kankan, Kindia, Labé, Mamou, and Nzérékoré)...
October 20, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/29048703/a-pilot-study-on-the-efficacy-and-safety-of-a-minimally-invasive-surgical-and-anesthetic-approach-for-ventricular-assist-device-implantation
#18
Jonida Bejko, Demetrio Pittarello, Gianclaudio Falasco, Guido Di Gregorio, Vincenzo Tarzia, Giulio Rizzoli, Dario Gregori, Gino Gerosa, Tomaso Bottio
BACKGROUND: The aim of our study was to compare 2 surgical and anesthetic approaches during ventricular assist device implantation. METHODS: 68 patients (50.4 ± 17.1 years old) were supported with the HeartWare® HVAD (32 patients) and the Jarvik 2000 VAD (36 patients) between January 2010 and August 2016. Two surgical techniques were applied: a minimally invasive approach with the aid of paravertebral-block (mini-invasive group, 41 patients) and a standard-surgical-approach with the aid of general anesthesia (27 patients)...
October 16, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/29046602/surveillance-at-sea-the-transactional-politics-of-border-control-in-the-aegean
#19
Huub Dijstelbloem, Rogier van Reekum, Willem Schinkel
The relationship between vision and action is a key element of both practices and conceptualizations of border surveillance in Europe. This article engages with what we call the 'operative vision' of surveillance at sea, specifically as performed by the border control apparatus in the Aegean. We analyse the political consequences of this operative vision by elaborating on three examples of fieldwork conducted in the Aegean and on the islands of Chios and Lesbos. One of the main aims is to bring the figure of the migrant back into the study of border technologies...
June 2017: Secur Dialogue
https://www.readbyqxmd.com/read/29046508/the-marcks-protein-amount-is-differently-regulated-by-calpain-during-toxic-effects-of-methylmercury-between-sh-sy5y-and-ea-hy926-cells
#20
Cuong Van Dao, Mitsuya Shiraishi, Atsushi Miyamoto
Methylmercury (MeHg) is an environmental pollutant that shows severe toxicity to humans and animals. However, the molecular mechanisms mediating MeHg toxicity are not completely understood. We have previously reported that the MARCKS protein is involved in the MeHg toxicity to SH-SY5Y neuroblastoma and EA.hy926 vascular endothelial cell lines. In addition, calpain, a Ca(2+)-dependent protease, is suggested to be associated with the MeHg toxicity. Because MARCKS is known as a substrate of calpain, we studied the relation between calpain activation and cleavage of MARCKS and its role in MeHg toxicity...
October 18, 2017: Journal of Veterinary Medical Science
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