Oliver I Brown, Katherine I Bridge, Sam Straw, Natallia Makava, Jason Scragg, Sunti Limumpornpetch, Cheukyau Luk, Jessica Smith, Anna Skromna, Alexander F Bruns, Piruthivi Sukumar, Lee D Roberts, Richard Cubbon, Klaus K Witte, Stephen Wheatcroft, Mark T Kearney
Microvascular endothelial cells (MVECs) have many critical roles, including control of vascular tone, regulation of thrombosis, and angiogenesis. Significant heterogeneity in endothelial cell (EC) genotype and phenotype depends on their vascular bed and host disease state. The ability to isolate MVECs from tissue-specific vascular beds and individual patient groups offers the opportunity to directly compare MVEC function in different disease states. Here, using subcutaneous adipose tissue (SAT) taken at the time of insertion of cardiac implantable electronic devices (CIED), we describe a method for the isolation of a pure population of functional human subcutaneous adipose tissue MVEC (hSATMVEC) and an experimental model of hSATMVEC-adipocyte cross-talk...
April 5, 2024: Journal of Visualized Experiments: JoVE