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https://www.readbyqxmd.com/read/27910804/sudden-cardiac-death-a-nationwide-cohort-study-among-the-young
#1
Bjarke Risgaard
Sudden cardiac death (SCD) is a tragic event affecting millions of individuals worldwide. Although several studies have investigated the epidemiology of SCD, these studies may have been affected by reporting and referral biases, which are reflected in the very different incidence rates and causes of deaths that have previously been reported. Among SCD victims aged < 36 years, inherited cardiac diseases are well known to play an important role. However, the extent to which inherited cardiac diseases also play a role in SCD victims aged < 50 years has not been completely described...
December 2016: Danish Medical Journal
https://www.readbyqxmd.com/read/27906832/clinical-validation-of-simultaneous-analysis-of-tacrolimus-cyclosporine-a-and-creatinine-in-dried-blood-spots-in-kidney-transplant-patients
#2
Herman Veenhof, Remco A Koster, Jan-Willem C Alffenaar, Stefan P Berger, Stephan J L Bakker, Daan J Touw
BACKROUND: Monitoring of creatinine and immunosuppressive drug concentrations such as tacrolimus (TaC) and cyclosporin A (CsA) is important in the outpatient follow-up of kidney transplant recipients. Monitoring by Dried Blood Spot (DBS) provides patients the opportunity to sample a drop of blood from a fingerprick at home, which can be send to the laboratory by mail. METHODS: We performed a clinical validation in which we compared measurements from whole blood samples obtained by venapuncture with measurements from DBS samples simultaneously obtained by fingerprick...
December 1, 2016: Transplantation
https://www.readbyqxmd.com/read/27903815/neonatal-vitamin-d-status-and-risk-of-multiple-sclerosis-a-population-based-case-control-study
#3
Nete Munk Nielsen, Kassandra L Munger, Nils Koch-Henriksen, David M Hougaard, Melinda Magyari, Kristian T Jørgensen, Marika Lundqvist, Jacob Simonsen, Tine Jess, Arieh Cohen, Egon Stenager, Alberto Ascherio
OBJECTIVE: As previous research has suggested that exposure to vitamin D insufficiency in utero may have relevance for the risk of multiple sclerosis (MS), we aimed to examine the direct association between level of neonatal vitamin D and risk of MS. METHODS: We carried out a matched case-control study. Dried blood spots samples (DBSS) belonging to 521 patients with MS were identified in the Danish Newborn Screening Biobank. For every patient with MS, 1-2 controls with the same sex and birth date were retrieved from the Biobank (n = 972)...
November 30, 2016: Neurology
https://www.readbyqxmd.com/read/27903736/t-and-b-cell-markers-in-dried-blood-spots-of-neonates-with-congenital-cytomegalovirus-infection-b-cell-numbers-at-birth-are-associated-with-long-term-outcomes
#4
Roberta Rovito, Marjolein J Korndewal, Menno C van Zelm, Dimitrios Ziagkos, Els Wessels, Mirjam van der Burg, Aloys C M Kroes, Anton W Langerak, Ann C T M Vossen
Congenital CMV infection (cCMV) is the most common congenital infection that can cause long-term impairment (LTI). The pathogenesis of LTI is not completely understood. Fetal immunity may play a role in controlling the infection and preventing LTI, although immune activation may also contribute to fetal immunopathology. In this study, we analyzed various molecular markers of T and B cell numbers in neonatal dried blood spots of 99 children with cCMV and 54 children without cCMV: δRec-ψJα signal joints on TCR excision circles, intron recombination signal sequence k-deleting element signal joints on Igκ-deleting recombination excision circles, genomic intron recombination signal sequence k-deleting element coding joint, genomic Vδ1-Jδ1, and Vδ2-Jδ1 rearrangements...
November 30, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27902602/sensitivity-and-specificity-of-dried-blood-spots-for-hiv-1-viral-load-quantification-a-laboratory-assessment-of-3-commercial-assays
#5
Pieter Pannus, Maarten Claus, Maria Mercedes Perez Gonzalez, Nathan Ford, Katrien Fransen
The use of dried blood spots (DBS) instead of plasma as a specimen type for HIV-1 viral load (VL) testing facilitates the decentralization of specimen collection and can increase access to VL testing in resource-limited settings. The performance of DBS for VL testing is lower, however, when compared to the gold standard sample type plasma. In this diagnostic accuracy study, we evaluated 3 VL assays with DBS.Participants were recruited between August 2012 and April 2015. Both plasma and DBS specimens were prepared and tested for HIV-1 VL with the Roche CAP/CTM HIV-1 test v2...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27900477/the-influence-of-seasonality-and-manufacturer-kit-lot-changes-on-17%C3%AE-hydroxyprogesterone-measurements-and-referral-rates-of-congenital-adrenal-hyperplasia-in-newborns
#6
Melissa Pearce, Erin Dauerer, A Gregory DiRienzo, Michele Caggana, Norma P Tavakoli
: Newborn screening for congenital adrenal hyperplasia (CAH) is performed by measuring the concentration of 17α-hydroxyprogesterone (17-OHP) in dried blood spots. Unfortunately, the level of 17-OHP varies due to multiple factors, and therefore, the false positive rate for the test is a challenge. We analyzed screening data from 2007 to 2015 to determine the effect of seasonal changes and manufacturer kit lot changes on 17-OHP values and on numbers of infants referred. Data from screening 2...
November 29, 2016: European Journal of Pediatrics
https://www.readbyqxmd.com/read/27895279/evaluation-of-immunoglobulin-g-responses-to-plasmodium-falciparum-and-plasmodium-vivax-in-malian-school-children-using-multiplex-bead-assay
#7
Eric Rogier, Delynn M Moss, Anna N Chard, Victoria Trinies, Seydou Doumbia, Matthew C Freeman, Patrick J Lammie
Malaria serology through assaying for IgG against Plasmodium spp. antigens provides evidence into the infection history for an individual. The multiplex bead assay (MBA) allows for detection of IgG against multiple Plasmodium spp., and can be especially useful in many regions where Plasmodium falciparum is of primary clinical focus, but other species are co-endemic. Dried blood spots were collected from 805 Malian children attending 42 elementary schools in the regions of Mopti, Sikasso, Koulikoro, and Bamako capital district, and IgG assayed by MBA...
November 28, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27879554/measles-and-rubella-seroprevalence-among-hiv-infected-and-uninfected-zambian-youth
#8
Catherine G Sutcliffe, Kelly Searle, Hellen K Matakala, Dip Biomed, Michelle Greenman, Kaitlin Rainwater-Lovett, Philip E Thuma, William J Moss
BACKGROUND: Measles and congenital rubella syndrome remain significant causes of morbidity and mortality despite available vaccines. HIV-infected youth may be at increased risk of measles because of greater waning immunity following vaccination. At a population level, they constitute a potentially large pool of susceptibles to measles and rubella. More data among HIV-infected youth in sub-Saharan Africa are needed to guide vaccination policy and control strategies. METHODS: This cross-sectional study was nested within two ongoing studies of malaria and HIV in Zambia...
November 22, 2016: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/27877170/comparison-of-s-stercoralis-serology-performed-on-dried-blood-spots-and-on-conventional-serum-samples
#9
Fabio Formenti, Dora Buonfrate, Rosanna Prandi, Monica Marquez, Cintia Caicedo, Eleonora Rizzi, Angel G Guevara, Yosselin Vicuña, Francisco R Huerlo, Francesca Perandin, Zeno Bisoffi, Mariella Anselmi
Background: Dried blood spots (DBS) are used for epidemiological surveys on infectious diseases in settings where limited resources are available. In fact, DBS can help to overcome logistic difficulties for the collection, transport and storage of biological specimens. Objective: To evaluate the accuracy of Strongyloides stercoralis serology performed on DBS. Methods: A survey was proposed to children attending a school in the village of Borbon, Ecuador, and to their parents/guardians. Each participant gave consent to the collection of both serum and DBS specimens...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27873432/low-perinatal-zinc-status-is-not-associated-with-the-risk-of-type-1-diabetes-in-children
#10
Julie N Kyvsgaard, Anne J Overgaard, Louise D Jacobsen, Steffen U Thorsen, Christian B Pipper, Thomas H Hansen, Søren Husted, Henrik B Mortensen, Flemming Pociot, Jannet Svensson
AIM: Immunologic events during fetal life may play a part in the pathogenesis of type 1 diabetes (T1D). As zinc is involved in immunologic processes, the purpose was to investigate perinatal zinc status and the later risk of developing T1D and association to age at onset. METHODS: A population-based case-control study based on data from Danish Childhood Diabetes Register and the Danish Newborn Screening Biobank. Cases and controls were matched by birth year and month...
November 22, 2016: Pediatric Diabetes
https://www.readbyqxmd.com/read/27873105/newborn-screening-for-severe-primary-immunodeficiency-diseases-in-sweden-a-2-year-pilot-trec-and-krec-screening-study
#11
Michela Barbaro, Annika Ohlsson, Stephan Borte, Susanne Jonsson, Rolf H Zetterström, Jovanka King, Jacek Winiarski, Ulrika von Döbeln, Lennart Hammarström
Newborn screening for severe primary immunodeficiencies (PID), characterized by T and/or B cell lymphopenia, was carried out in a pilot program in the Stockholm County, Sweden, over a 2-year period, encompassing 58,834 children. T cell receptor excision circles (TREC) and kappa-deleting recombination excision circles (KREC) were measured simultaneously using a quantitative PCR-based method on DNA extracted from dried blood spots (DBS), with beta-actin serving as a quality control for DNA quantity. Diagnostic cutoff levels enabling identification of newborns with milder and reversible T and/or B cell lymphopenia were also evaluated...
November 21, 2016: Journal of Clinical Immunology
https://www.readbyqxmd.com/read/27858124/fluorescent-adduct-formation-with-terbium-a-novel-strategy-for-transferrin-glycoform-identification-in-human-body-fluids-and-carbohydrate-deficient-transferrin-hplc-method-validation
#12
Daniela Sorio, Elio Franco De Palo, Anna Bertaso, Federica Bortolotti, Franco Tagliaro
This paper puts forward a new method for the transferrin (Tf) glycoform analysis in body fluids that involves the formation of a transferrin-terbium fluorescent adduct (TfFluo). The key idea is to validate the analytical procedure for carbohydrate-deficient transferrin (CDT), a traditional biochemical serum marker to identify chronic alcohol abuse. Terbium added to a human body-fluid sample produced TfFluo. Anion exchange HPLC technique, with fluorescence detection (λ exc 298 nm and λ em 550 nm), permitted clear separation and identification of Tf glycoform peaks without any interfering signals, allowing selective Tf sialoforms analysis in human serum and body fluids (cadaveric blood, cerebrospinal fluid, and dried blood spots) hampered for routine test...
November 17, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27852877/community-seroprevalence-survey-for-yaws-and-trachoma-in-the-western-division-of-fiji
#13
Naomi Cocks, Merelesita Rainima-Qaniuci, Chelsea Yalen, Colin Macleod, Apisalome Nakolinivalu, Stephanie Migchelsen, Chrissy H Roberts, Robert Butcher, Mike Kama, David Mabey, Michael Marks
INTRODUCTION: Both yaws and trachoma are endemic in several countries in the Pacific. In co-endemic countries there may be potential synergies between both control programmes. METHODS: We undertook a cluster randomised trachoma and yaws seroprevalence survey of children in the Western Division of Fiji. Children were examined for skin lesions consistent with active yaws. A dried blood spot was collected which was tested using the Treponema pallidum particle agglutination (TPPA) test and an ELISA to detect antibodies against Pgp3...
November 16, 2016: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27846247/islet-autoantibody-measurements-from-dried-blood-spots-on-filter-paper-strongly-correlate-to-serum-levels
#14
Kimber M Simmons, Aimon K Alkanani, Kristen A McDaniel, Christopher Goyne, Dongmei Miao, Zhiyuan Zhao, Liping Yu, Aaron W Michels
Type 1 diabetes (T1D) is increasing in incidence and predictable with measurement of serum islet autoantibodies (iAb) years prior to clinical disease onset. Identifying iAb positive individuals reduces diabetic ketoacidosis and identifies individuals for T1D prevention trials. However, large scale screening for iAb remains challenging as assays have varying sensitivities and specificities, insulin autoantibodies remain difficult to measure and venipuncture is generally required to obtain serum. We developed an approach to reliably measure all four major iAb, including insulin autoantibodies, from dried blood spots (DBS) on filter-paper...
2016: PloS One
https://www.readbyqxmd.com/read/27832731/screening-fabry-s-disease-in-chronic-kidney-disease-patients-not-on-dialysis-a-multicenter-study
#15
Yavuz Yeniçerioğlu, Hakan Akdam, Belda Dursun, Alper Alp, Funda Sağlam Eyiler, Davut Akın, Yelda Gün, Bülent Hüddam, Mehmet Batmazoğlu, Dilek Gibyeli Genek, Serhat Pirinççi, İsmail Rıfkı Ersoy, Atilla Üzüm, Zeki Soypaçacı, Mehmet Tanrısev, Hülya Çolak, Sibel Demiral Sezer, Gökay Bozkurt, Utku Oğan Akyıldız, Ayşe İpek Akyüz Ünsal, Mustafa Ünübol, Meltem Uslu, Ufuk Eryılmaz, Ceren Günel, İbrahim Meteoğlu, İrfan Yavaşoğlu, Alparslan Ünsal, Harun Akar, Pınar Okyay
OBJECTIVES: Fabry's disease is an X-linked inherited, rare, progressive, lysosomal storage disorder, affecting multiple organs due to the deficient activity of α-galactosidase A (α-Gal A) enzyme. The prevalence has been reported to be 0.15-1% in hemodialysis patients; however, the information on the prevalence in chronic kidney disease not on dialysis is lacking. This study aimed to determine the prevalence of Fabry's disease in chronic kidney disease. METHODS: The patients older than 18 years, enclosing KDIGO 2012 chronic kidney disease definitions, not on dialysis, were enrolled...
November 10, 2016: Renal Failure
https://www.readbyqxmd.com/read/27822645/comparison-of-paper-spray-mass-spectrometry-analysis-of-dried-blood-spots-from-devices-used-for-in-field-collection-of-clinical-samples
#16
Karen E Yannell, Kristina R Kesely, Huynh Dinh Chien, Candice B Kissinger, R Graham Cooks
Paper spray (PS) is an ambient ionization technique applicable to ionizing analytes from untreated dried biofluid samples. In-field sample analysis could benefit from the capability to use a finger prick of blood to measure drugs in whole blood at low cost and in a short time. Some studies may require specialized blood collection devices that can be used in remote areas. In this study, four different dried blood spot (DBS) devices are used with PS sources and tested for rapid quantification of imatinib and N-desmethyl-imatinib...
November 7, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27820845/dry-blood-spots-a-reliable-method-for-measurement-of-hepatitis-b-viral-load-in-resource-limited-settings
#17
Kathrine Stene-Johansen, Nadeem Yaqoob, Joakim Overbo, Hanna Aberra, Hailemichael Desalegn, Nega Berhe, Asgeir Johannessen
BACKGROUND & AIMS: Hepatitis B virus (HBV) quantification is essential in the management of chronic hepatitis B, both to determine treatment eligibility and in the monitoring of treatment effect. This test, however, is rarely available in resource-limited settings due to high costs and stringent requirements for shipment and storage of plasma. Dried Blood Spots (DBS) can be a convenient alternative to plasma, but its use for HBV monitoring has not been investigated under real-life conditions in Africa...
2016: PloS One
https://www.readbyqxmd.com/read/27819061/use-of-dried-blood-spots-to-elucidate-full-length-transmitted-founder-hiv-1-genomes
#18
Jesus F Salazar-Gonzalez, Maria G Salazar, Damien C Tully, Colin B Ogilvie, Gerald H Learn, Todd M Allen, Sonya L Heath, Paul Goepfert, Katharine J Bar
BACKGROUND: Identification of HIV-1 genomes responsible for establishing clinical infection in newly infected individuals is fundamental to prevention and pathogenesis research. Processing, storage, and transportation of the clinical samples required to perform these virologic assays in resource-limited settings requires challenging venipuncture and cold chain logistics. Here, we validate the use of dried-blood spots (DBS) as a simple and convenient alternative to collecting and storing frozen plasma...
2016: Pathogens & Immunity
https://www.readbyqxmd.com/read/27816556/aflatoxin-b1-lysine-adduct-in-dried-blood-spot-samples-of-animals-and-humans
#19
Kathy S Xue, Wenjie Cai, Lili Tang, Jia-Sheng Wang
Dried blood spots (DBS) were proposed as potentially viable method for exposure assessment of environmental toxicants in infant and young children. For this study, we validated an experimental protocol to quantify AFB1-lysine adduct in DBS samples of AFB1-treated F344 rats, as well as samples from human field study. Significant dose-response relationships in AFB1-lysine adduct formation were found in DBS samples of rats treated with single- and repeated-dose AFB1. AFB1-lysine levels in DBS samples were highly correlated with corresponding serum sample levels...
November 2, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27814620/mucopolysaccharidosis-vi-pathophysiology-diagnosis-and-treatment
#20
Paul Harmatz, Renee Shediac
Mucopolysaccharidosis VI (MPS VI), or Maroteaux-Lamy syndrome, is an autosomal recessive lysosomal storage disorder caused by deficient activity of the enzyme arylsulfatase B (ASB). Progressive accumulation of glycosaminoglycans (GAGs) in organs and tissues leads to the development of multisystem clinical manifestations. The presentation of MPS VI is genotypically and phenotypically diverse, with a large number of potential disease-causing mutations and a phenotypic spectrum ranging from very slowly to very rapidly progressing disease...
January 1, 2017: Frontiers in Bioscience (Landmark Edition)
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