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https://www.readbyqxmd.com/read/28441697/hypertension-management-in-a-population-of-older-adults-in-rural-south-africa
#1
Thiago Veiga Jardim, Sheridan Reiger, Shafika Abrahams-Gessel, F Xavier Gomez-Olive, Ryan G Wagner, Alisha Wade, Till W Bärnighausen, Joshua Salomon, Stephen Tollman, Thomas A Gaziano
OBJECTIVE: Assess awareness, treatment, and control of hypertension, as an indication of its management, in rural South Africa, especially regarding modifiers of these variables. METHODS: A population-representative sample of adults aged at least 40 years residing in the rural Agincourt subdistrict (Mpumalanga Province) covered by a long-term health and sociodemographic surveillance system was recruited. In-person interviews, physical exams, and dried blood spots were collected...
June 2017: Journal of Hypertension
https://www.readbyqxmd.com/read/28438881/prevalence-of-congenital-cytomegalovirus-infection-assessed-through-viral-genome-detection-in-dried-blood-spots-in-children-with-autism-spectrum-disorders
#2
Ivan Gentile, Emanuela Zappulo, Maria Pia Riccio, Sandro Binda, Laura Bubba, Laura Pellegrinelli, Domenico Scognamiglio, Francesca Operto, Lucia Margari, Guglielmo Borgia, Carmela Bravaccio
BACKGROUND/AIM: Autism spectrum disorders (ASD) are neurodevelopmental disorders without a definitive etiology in most cases. Environmental factors, such as viral infections, have been linked with anomalies in brain growth, neuronal development, and functional connectivity. Congenital cytomegalovirus (CMV) infection has been associated with the onset of ASD in several case reports. The aim of this study was to evaluate the prevalence of congenital CMV infection in children with ASD and in healthy controls...
May 2017: In Vivo
https://www.readbyqxmd.com/read/28433475/targeted-population-screening-of-late-onset-pompe-disease-in-unspecified-myopathy-patients-for-korean-population
#3
Jung Hwan Lee, Jin-Hong Shin, Hyung Jun Park, Sook Za Kim, Young Mi Jeon, Hye Kyoung Kim, Dae-Seong Kim, Young-Chul Choi
We performed targeted population screening of late onset Pompe disease (LOPD) in unspecified myopathy patients, because early diagnosis is difficult due to its heterogeneous clinical features. We prospectively enrolled 90 unrelated myopathic patients who had one or more signs out of five LOPD-like clinical findings (proximal weakness, axial weakness, lingual weakness, respiratory difficulty, idiopathic hyperCKemia). Acid alpha glucosidase activity was evaluated with dried blood spot and mixed leukocyte simultaneously...
March 10, 2017: Neuromuscular Disorders: NMD
https://www.readbyqxmd.com/read/28431423/blood-bupivacaine-concentrations-after-a-combined-single-shot-sciatic-block-and-a-continuous-femoral-nerve-block-in-pediatric-patients-a-prospective-observational-study
#4
Santhanam Suresh, Gildasio S De Oliveira
We evaluated blood bupivacaine concentrations in children having a single-shot sciatic and continuous femoral blocks after anterior cruciate ligament repair. Dried blood spot samples were analyzed for bupivacaine levels at 0, 5, 15, 30, 60, and 120 minutes and 4, 24, and 48 hours. The highest 99% upper confidence interval limit was 135 ng/mL at the 4-hour evaluation point. The 99% upper confidence interval was below potentially toxic levels (1500 ng/mL) across all sampling times. The risk of local anesthetic toxicity in pediatric patients receiving single-shot sciatic and continuous femoral nerve blocks is very low...
May 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28428354/multiplex-tandem-mass-spectrometry-enzymatic-activity-assay-for-newborn-screening-of-the-mucopolysaccharidoses-and-type-2-neuronal-ceroid-lipofuscinosis
#5
Yang Liu, Fan Yi, Arun Babu Kumar, Naveen Kumar Chennamaneni, Xinying Hong, C Ronald Scott, Michael H Gelb, Frantisek Turecek
BACKGROUND: We expanded the use of tandem mass spectrometry combined with liquid chromatography (LC-MS/MS) for multiplex newborn screening of seven lysosomal enzymes in dried blood spots (DBS). The new assays are for enzymes responsible for the mucopolysaccharidoses (MPS-I, -II, -IIIB, -IVA, -VI, and -VII) and type 2 neuronal ceroid lipofuscinosis (LINCL). METHODS: New substrates were prepared and char-acterized for tripeptidyl peptidase 1 (TPP1), α-N-acetylglucosaminidase (NAGLU), and lysosomal β-glucuronidase (GUSB)...
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28427917/application-of-the-isoniazid-assay-in-dried-blood-spots-using-the-ultra-performance-liquid-chromatography-tandem-mass-spectrometry
#6
Kyunghoon Lee, Sun-Hee Jun, Moon-Suk Choi, Sang Hoon Song, Jong Sun Park, Jae Ho Lee, Kyoung Un Park, Junghan Song
OBJECTIVES: Therapeutic drug monitoring (TDM) of anti-tuberculosis (TB) drugs is important for proper treatment of TB. Dried blood spots (DBSs) are widely used for TDM because of their several advantages. Rifampicin and pyrazinamide assays with DBSs have already been developed. However, isoniazid (INH) assay for capillary DBSs have not been reported because of INH instability. We developed an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for measuring INH concentrations in venous and capillary DBSs...
April 17, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28422942/development-of-tandem-mass-spectrometry-based-creatinine-measurement-using-dried-blood-spot-for-newborn-mass-screening
#7
Masaru Nakano, Osamu Uemura, Masataka Honda, Tetsuya Ito, Yoko Nakajima, Shinji Saitoh
BACKGROUND: Congenital anomalies of the kidney and urinary tract (CAKUT) are the most prevalent etiology of pediatric chronic kidney disease (CKD). However, no robust mass screening methods have been developed to detect patients with CAKUT, making early intervention to prevent progressive renal failure challenging. METHODS: We applied tandem mass spectrometry (MS/MS) to measure the creatinine (Cr) value from dried blood spot (DBS) analysis, which has been used for newborn mass screening...
April 19, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28418986/performance-of-virological-testing-for-early-infant-diagnosis-a-systematic-review
#8
Divya Mallampati, Nathan Ford, Alisse Hanaford, Nandita Sugandhi, Martina Penazzato
BACKGROUND: Improved access to both maternal antiretroviral therapy (ART) and infant prophylaxis may have an impact on the performance of virological assays for diagnosis of HIV infection in infants. This systematic review was performed to assess the diagnostic accuracy of virological testing at birth as well as the performance of virological testing on dried-blood spots at 6-weeks amongst HIV- and ARV-exposed infants. METHODS: A systematic review was performed for studies published between January 1, 2009 and January 30, 2015...
April 7, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28418232/high-pre-exposure-prophylaxis-uptake-and-early-adherence-among-men-who-have-sex-with-men-and-transgender-women-at-risk-for-hiv-infection-the-prep-brasil-demonstration-project
#9
Brenda Hoagland, Ronaldo I Moreira, Raquel B De Boni, Esper G Kallas, José Valdez Madruga, Ricardo Vasconcelos, Silvia Goulart, Thiago S Torres, Luana M S Marins, Peter L Anderson, Paula M Luz, Iuri da Costa Leite, Albert Y Liu, Valdilea G Veloso, Beatriz Grinsztejn
INTRODUCTION: The efficacy of pre-exposure prophylaxis (PrEP) in preventing sexual acquisition of human immunodeficiency virus (HIV) is well established. Little is known about the feasibility of PrEP implementation in middle-income settings with concentrated epidemics among men who have sex with men (MSM) and transgender women (TGW). METHODS: PrEP Brasil is a prospective, multicentre, open-label demonstration project assessing PrEP delivery in the context of the Brazilian Public Health System...
April 6, 2017: Journal of the International AIDS Society
https://www.readbyqxmd.com/read/28413201/detection-of-%C3%AE-thalassaemia-in-neonates-on-cord-blood-and-dried-blood-spot-samples-by-capillary-electrophoresis
#10
H Alauddin, M Langa, M Mohd Yusoff, R Z A Raja Sabudin, A Ithnin, N F Abdul Razak, N H Sardi, N H Hussin
INTRODUCTION: Haemoglobin Bart's (Hb Bart's) level is associated with α-thalassaemia traits in neonates, enabling early diagnosis of α-thalassaemia. The study aimed to detect and quantify the Hb Bart's using Cord Blood (CB) and CE Neonat Fast Hb (NF) progammes on fresh and dried blood spot (DBS) specimen respectively by capillary electrophoresis (CE). METHODS: Capillarys Hemoglobin (E) Kit (for CB) and Capillarys Neonat Hb Kit (for NF) were used to detect and quantify Hb Bart's by CE in fresh cord blood and dried blood spot (DBS) specimens respectively...
April 2017: Malaysian Journal of Pathology
https://www.readbyqxmd.com/read/28412875/amino-acid-and-acylcarnitine-profiles-in-perinatal-asphyxia-a-case-control-study
#11
Ola Galal El-Farghali, Mohamed Sami El-Chimi, Heba Salah El-Abd, Eman El-Desouky
OBJECTIVE: To estimate cord blood amino acid and acylcarnitine levels in term newborns exposed to perinatal asphyxia. MATERIALS AND METHODS: We studied 45 asphyxiated term newborns (cases) and 20 gestational age-matched healthy newborns (control). Sixteen cases developed HIE according to clinical scoring and amplitude-integrated electroencephalography. Asphyxiated cases were accordingly subdivided into: HIE group (n = 16) and Asphyxia group (n = 29). Amino acid and acylcarnitine levels were measured in cord blood dried spot samples from all newborns using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS)...
April 16, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28409012/enhancing-the-diagnosis-of-fabry-disease-in-cardiology-with-a-targeted-information-a-before-after-control-impact-study
#12
Anne-Louise Savary, Remy Morello, Carole Brasse-Lagnel, Paul Milliez, Soumeya Bekri, Fabien Labombarda
BACKGROUND: Cardiac complications in Fabry disease are frequent and dominated by a high frequency of left ventricular hypertrophy; therefore, cardiologists may have an essential role in screening for this disease. Providing cardiologists with targeted information on Fabry disease would be valuable and could reduce both diagnostic and therapeutic delays. The aim of this study was to evaluate the efficiency of such strategy for Fabry screening. METHODS: We conducted a before-after control-impact study by comparing observations made before and after targeted information on Fabry disease among cardiologists...
2017: Open Heart
https://www.readbyqxmd.com/read/28396248/age-and-gender-specific-reference-intervals-for-lysosomal-enzymes-in-dried-blood-spot-samples-a-study-in-indian-population
#13
Manjunath Supriya, Tanima De, Rita Christopher
OBJECTIVES: The study aimed to establish age and gender-specific reference values for the activities of lysosomal enzymes (acid α-galactosidase [GLA], acid β-glucocerebrosidase [GBA], acid α-glucosidase [GAA], acid sphingomyelinase [ASM] and galactocerebrosidase [GALC]) in dried blood spots (DBS) of Indian population. DESIGN AND METHODS: A total of 3797 healthy Indian subjects (1456 females and 2341 males) aged from 2days to 60years were selected for the study...
April 7, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28396038/severe-reduction-of-blood-lysosomal-acid-lipase-activity-in-cryptogenic-cirrhosis-a-nationwide-multicentre-cohort-study
#14
Francesco Angelico, Stefano Ginanni Corradini, Daniele Pastori, Silvia Fargion, Anna Ludovica Fracanzani, Mario Angelico, Luigi Bolondi, Giulia Tozzi, Pietro Luigi Pujatti, Giancarlo Labbadia, Gino Roberto Corazza, Maurizio Averna, Francesco Perticone, Giuseppe Croce, Marcello Persico, Tommaso Bucci, Francesco Baratta, Licia Polimeni, Maria Del Ben, Francesco Violi
BACKGROUND AND AIMS: Blood lysosomal acid lipase (LAL) is reduced in non-alcoholic steatohepatitis, which is the major cause of cryptogenic cirrhosis (CC); few data on LAL activity in CC do exist. We investigated LAL activity in a cohort of patients with liver cirrhosis. METHODS: This is a multicentre cohort study including 274 patients with liver cirrhosis of different aetiology from 19 centres of Internal Medicine, Gastroenterology and Hepatology distributed throughout Italy...
March 31, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28395245/validation-of-a-dried-blood-spot-method-for-therapeutic-drug-monitoring-of-citalopram-mirtazapine-and-risperidone-and-its-active-metabolite-9-hydroxyrisperidone-using-hplc-ms
#15
Johanna Weber, Stefanie Oberfeld, Andrea Bonse, Klaus Telger, Rainer Lingg, Georg Hempel
Citalopram, mirtazapine and risperidone are frequently prescribed for psychiatric illnesses such as depression and psychosis or for aggressive behavior in elderly patients with dementia. The plasma concentrations vary greatly between patients, especially in elderly patients. Thus, therapeutic drug monitoring (TDM) increases the safety of antipsychotic treatment and a more rapid response to treatment may be achieved. To facilitate TDM, the objectives of this study were to develop and validate a reliable dried blood spot method to simultaneously quantify citalopram, mirtazapine and risperidone including its active metabolite 9-hydroxyrisperidone...
June 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28393579/clinical-perspectives-of-dried-blood-spot-protein-quantification-using-mass-spectrometry-methods
#16
Sylvain Lehmann, Alexia Picas, Laurent Tiers, Jerome Vialaret, Christophe Hirtz
Although dried blood spot (DBS) sampling methods have been used since the 1960s, they have recently attracted renewed interest because of the development of new clinical applications. In addition to their other advantages, DBS methods can now be used to quantify many blood proteins using the latest highly sensitive and robust, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) approaches such as multiple reaction monitoring. The DBS blood sampling approach could provide a useful alternative means of conducting blood sampling for routine clinical purposes and patients' follow-up...
April 10, 2017: Critical Reviews in Clinical Laboratory Sciences
https://www.readbyqxmd.com/read/28391006/capillary-blood-collected-on-volumetric-absorptive-microsampling-vams-device-for-monitoring-hydroxychloroquine-in-rheumatoid-arthritis-patients
#17
Ying Qu, Kelley Brady, Robert Apilado, Tyler O'Malley, Smitha Reddy, Puja Chitkara, Claudia Ibarra, Roberta Vezza Alexander, Thierry Dervieux
A novel technique for collection of capillary blood, termed volumetric absorptive microsampling (VAMS), has been recently cleared by the FDA for collection of human blood. VAMS absorbs a fixed volume of blood (10μl) and overcomes area bias and homogeneity issues associated with dried blood spot (DBS). This study is the application of VAMS for therapeutic drug monitoring (TDM) in human capillary blood. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) workflow for analysis of VAMS sample was developed and validated...
June 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28388902/therapeutic-efficacy-and-artemisinin-resistance-in-northern-myanmar-evidence-from-in-vivo-and-molecular-marker-studies
#18
Moe Kyaw Myint, Charlotte Rasmussen, Aung Thi, Dorina Bustos, Pascal Ringwald, Khin Lin
BACKGROUND: In Myanmar, three types of artemisinin-based combination therapy (ACT) are recommended as first-line treatment of uncomplicated falciparum malaria: artemether-lumefantrine (AL), artesunate-mefloquine (AS + MQ), and dihydroartemisinin-piperaquine (DP). Resistance to both artemisinins and ACT partner drugs has been reported from the Greater Mekong Sub-region, and regular efficacy monitoring of the recommended ACT is conducted in Myanmar. This paper reports on results from studies to monitor the efficacy of the three forms of ACT in sentinel sites in northern Myanmar, and investigations of mutations in the Kelch13 (k13) propeller domain...
April 7, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28381283/consolidating-emerging-evidence-surrounding-hivst-and-hivss-a-rapid-systematic-mapping-protocol
#19
T Charles Witzel, Peter Weatherburn, Fiona M Burns, Cheryl C Johnson, Carmen Figueroa, Alison J Rodger
BACKGROUND: HIV self-testing (HIVST) is becoming popular with policy makers and commissioners globally, with a key aim of expanding access through reducing barriers to testing for individuals at risk of HIV infection. HIV self-sampling (HIVSS) was available previously to self-testing but was confined mainly to the USA and the UK. It remains to be seen whether the momentum behind HIVST will also energise efforts to expand HIVSS. Recent years have seen a rapid growth in the type of evidence related to these interventions as well as several systematic reviews...
April 5, 2017: Systematic Reviews
https://www.readbyqxmd.com/read/28381232/detection-of-newly-produced-t-and-b-lymphocytes-by-digital-pcr-in-blood-stored-dry-on-nylon-flocked-swabs
#20
Marion Vaglio Tessitore, Alessandra Sottini, Aldo M Roccaro, Claudia Ghidini, Simona Bernardi, Giovanni Martellosio, Federico Serana, Luisa Imberti
BACKGROUND: A normal number of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs) is considered a biomarker for adequate new T- and B-cell production. In newborns, detection of TRECs and KRECs by real time PCR from dried blood spotted on filter paper is used for the screening of severe immunodeficiency. In adults, elderly and during diseases, where the number of TRECs is lower than in newborns and children, a large amount of DNA and a sensitive method of amplification are necessary to identify newly produced lymphocytes...
April 5, 2017: Journal of Translational Medicine
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