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dried blood spot

Artem D Rogachev, Olga I Yarovaya, Sergey V Ankov, Mikhail V Khvostov, Tatyana G Tolstikova, Andrey G Pokrovsky, Nariman F Salakhutdinov
A fast, selective and sensitive procedure for quantitation of the camphor-based anti-influenza agent camphecene in whole rat blood was developed and validated using dried blood spots and LC-MS/MS. The method was validated according to recommendations of the FDA and EMA in terms of selectivity, linearity, accuracy, precision, recovery, matrix factor, stability, and carry-over. Sample preparation included spotting 20μL of whole blood taken from the tail vein onto the paper, drying and extracting the analyte, followed by evaporation of the solvent and analysis of the residue...
October 11, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Nick Verougstraete, Bruno Lapauw, Sara Van Aken, Joris Delanghe, Christophe Stove, Veronique Stove
BACKGROUND: Microsampling techniques have several advantages over traditional blood collection. Dried blood spot (DBS) sampling and blood collection with heparinized capillaries are the standard techniques. Volumetric absorptive microsampling (VAMS) is a novel technique that collects a fixed volume of blood by applying an absorbent tip to a blood drop. In the present study we explored the feasibility of HbA1c monitoring with VAMS sampling at home and analysis in the laboratory. METHODS: Diabetic patients were enrolled in this study during consultation with the endocrinologist...
October 12, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
Sara Stinca, Maria Andersson, Sandra Weibel, Isabelle Aeberli-Herter, Ralph Fingerhut, Sueppong Gowachirapant, Sonja Y Hess, Nidhi Jaiswal, Tomislav Jukić, Zvonko Kusic, Ngoako Solomon Mabapa, Ashwini Kumar Nepal, Teofilo O L San Luis, Jia Qing Zhen, Michael Bruce Zimmermann
CONTEXT: Thyroglobulin (Tg) may be a sensitive biomarker of iodine nutrition in pregnant women (PW). A dried blood spot (DBS) assay would simplify collection and transport in field studies. OBJECTIVES: Our aims were to: 1) establish a reference range for DBS-Tg in iodine-sufficient PW; 2) test this reference range in PW with a range of iodine intakes; and 3) determine if co-measurement of Tg antibodies (Ab) is necessary to define population iodine status. Design, Settings and Participants: Standardized cross-sectional studies in 3870 PW from 11 countries with iodine intakes from deficient to more-than-adequate...
October 12, 2016: Journal of Clinical Endocrinology and Metabolism
Francyne Kubaski, Robert W Mason, Akiko Nakatomi, Haruo Shintaku, Li Xie, Naomi N van Vlies, Heather Church, Roberto Giugliani, Hironori Kobayashi, Seiji Yamaguchi, Yasuyuki Suzuki, Tadao Orii, Toshiyuki Fukao, Adriana M Montaño, Shunji Tomatsu
BACKGROUND: Mucopolysaccharidoses (MPS) are a group of inborn errors of metabolism that are progressive and usually result in irreversible skeletal, visceral, and/or brain damage, highlighting a need for early diagnosis. METHODS: This pilot study analyzed 2862 dried blood spots (DBS) from newborns and 14 DBS from newborn patients with MPS (MPS I, n = 7; MPS II, n = 2; MPS III, n = 5). Disaccharides were produced from polymer GAGs by digestion with chondroitinase B, heparitinase, and keratanase II...
October 7, 2016: Journal of Inherited Metabolic Disease
P Ferro, R Ventura, C Pérez-Mañá, M Farré, J Segura
Since the appearance of recombinant human growth hormone (rhGH) in the 80's, its expansion and acquisition through the black market has increased, so the detection of its abuse continues to be a challenge at present. New biomarkers that are more reliable and sensitive, allowing a larger detection window, are still needed. In this line, Fibronectin 1 (FN1) has been proposed as potential genetic and protein biomarker of rhGH abuse in peripheral blood lymphocytes, serum and plasma. However, logistic problems associated with current blood collection in sports drug testing points towards potential new alternative matrices that could be good candidates to be evaluated...
October 7, 2016: Drug Testing and Analysis
Fabien Taieb, Tran Hong Tram, Hien Thi Ho, Van Anh Pham, Lan Nguyen, Ban Hien Pham, Linh An Tong, Edouard Tuaillon, Eric Delaporte, Anh Tuan Nguyen, Duc Duong Bui, NhanThi Do, Yoann Madec
Background.  Although it is the best method to detect early therapeutic failure, viral load (VL) monitoring is still not widely available in many resource-limited settings because of difficulties in specimen transfer, personnel shortage, and insufficient laboratory infrastructures. Dried blood spot (DBS) use, which was introduced in the latest World Health Organization recommendations, can overcome these difficulties. This evaluation aimed at validating VL measurement in DBS, in a laboratory without previous DBS experience and in routine testing conditions...
September 2016: Open Forum Infectious Diseases
Ameena E Goga, Thu-Ha Dinh, Debra J Jackson, Carl J Lombard, Adrian Puren, Gayle Sherman, Vundli Ramokolo, Selamawit Woldesenbet, Tanya Doherty, Nobuntu Noveve, Vuyolwethu Magasana, Yagespari Singh, Trisha Ramraj, Sanjana Bhardwaj, Yogan Pillay
BACKGROUND: Eliminating mother-to-child transmission of HIV (EMTCT), defined as ≤50 infant HIV infections per 100 000 live births, is a global priority. Since 2011 policies to prevent mother-to-child transmission of HIV (PMTCT) shifted from maternal antiretroviral (ARV) treatment or prophylaxis contingent on CD4 cell count to lifelong maternal ARV treatment (cART). We sought to measure progress with early (4-8 weeks postpartum) MTCT prevention and elimination, 2011-2013, at national and sub-national levels in South Africa, a high antenatal HIV prevalence setting ( ≈ 29%), where early MTCT was 3...
December 2016: Journal of Global Health
Nele Sadones, Elien Van Bever, Luc Van Bortel, Willy E Lambert, Christophe P Stove
We evaluated the applicability of a validated GC-MS method for the determination of gabapentin in dried blood spots (DBS). Important for the acceptance of DBS sampling as an alternative sampling strategy is the possibility to base solid conclusions on the quantification. Therefore, bridging studies -studies in which the correlation between both DBS and a reference matrix (e.g. serum) is evaluated statistically- need to be conducted. To this end, a comparative study was set up to quantify gabapentin in both blood (DBS) and serum samples...
September 26, 2016: Journal of Pharmaceutical and Biomedical Analysis
Maira Ferreira Cicero, Nathalia Mantovani Pena, Luiz Claudio Santana, Rafael Arnold, Rafael Gonçalves Azevedo, Élcio de Souza Leal, Ricardo Sobhie Diaz, Shirley Vasconcelos Komninakis
BACKGROUND: The Hepatitis Delta Virus (HDV) can increase the incidence of fulminant hepatitis. For this infection occurs, the host must also be infected with Hepatitis B Virus. Previous studies demonstrated the endemicity and near exclusivity of this infection in the Amazon region, and as a consequence of the difficulty in accessing this area we used dried blood spots (DBS) in sample collection. The aims of this study were to investigate the presence of recombination, to analyze the epidemiology, ancestry and evolutionary pressures on HDV in Brazil...
September 29, 2016: BMC Infectious Diseases
Katherine M Livingstone, Carlos Celis-Morales, Ulrich Hoeller, Christina P Lambrinou, George Moschonis, Anna L Macready, Rosalind Fallaize, Manuela Baur, Franz F Roos, Igor Bendik, Keith Grimaldi, Santiago Navas-Carretero, Rodrigo San-Cristobal, Peter Weber, Christian A Drevon, Yannis Manios, Iwona Traczyk, Eileen R Gibney, Julie A Lovegrove, Wim H Saris, Hannelore Daniel, Mike Gibney, J Alfredo Martinez, Lorraine Brennan, Tom R Hill, John C Mathers
SCOPE: Little is known about diet- and environment-gene interactions on 25-hydroxyvitamin D (25(OH)D concentration. This cross-sectional study aimed to investigate i) predictors of 25(OH)D concentration and relationships with vitamin D genotypes and ii) whether dietary vitamin D intake and sunlight exposure modified these relationships. METHODS AND RESULTS: Participants from the Food4Me study (n = 1312; age 18-79) were genotyped for vitamin D receptor (VDR) and vitamin D binding protein at baseline and a genetic risk score was calculated...
September 28, 2016: Molecular Nutrition & Food Research
Belinda K Mössner, Benjamin Staugaard, Janne Jensen, Søren Thue Lillevang, Peer B Christensen, Dorte Kinggaard Holm
AIM: To detect chronic hepatitis B (CHB), chronic hepatitis C (CHC) and human immunodeficiency virus (HIV) infections in dried blood spot (DBS) and compare these samples to venous blood sampling in real-life. METHODS: We included prospective patients with known viral infections from drug treatment centers, a prison and outpatient clinics and included blood donors as negative controls. Five drops of finger capillary blood were spotted on filter paper, and a venous blood sample was obtained...
September 7, 2016: World Journal of Gastroenterology: WJG
Pere Gelabert, Marcela Sandoval-Velasco, Iñigo Olalde, Rosa Fregel, Adrien Rieux, Raül Escosa, Carles Aranda, Krijn Paaijmans, Ivo Mueller, M Thomas P Gilbert, Carles Lalueza-Fox
Phylogenetic analysis of Plasmodium parasites has indicated that their modern-day distribution is a result of a series of human-mediated dispersals involving transport between Africa, Europe, America, and Asia. A major outstanding question is the phylogenetic affinity of the malaria causing parasites Plasmodium vivax and falciparum in historic southern Europe-where it was endemic until the mid-20th century, after which it was eradicated across the region. Resolving the identity of these parasites will be critical for answering several hypotheses on the malaria dispersal...
October 11, 2016: Proceedings of the National Academy of Sciences of the United States of America
Sandra Aguayo-Patrón, Lizbeth Beltrán-Sauceda, Ana María Calderón de la Barca
Genotyping of HLA-DQ2 and DQ8 haplotypes is important for diagnosis or for screening of early risk detection of celiac disease or type 1 diabetes. Usually, venous blood DNA extraction and expensive and time consuming amplification are used, that hinder population-wide studies. We assayed a friendly HLA-DQ2 and DQ8 genotyping procedure using a combination of DNA from dried blood spot (DBS) and duplex allele-specific qPCR amplification using SYBR Green. DNA was extracted using home-made buffers and compared to an extraction commercial kit...
November 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Olcay Ünver, Nilüfer Eldeş Hacıfazlıoğlu, Elif Karatoprak, Ayfer Sakarya Güneş, Güneş Sağer, Büşra Kutlubay, Gülhan Sözen, Sema Saltık, Kutluhan Yılmaz, Bülent Kara, Dilşad Türkdoğan
The aim of this multicenter study was to screen for late-onset Pompe disease in high-risk children with limb-girdle muscle weakness and nonspecific hyperCKemia using the dried blood spot (DBS) test. Seventy-two children from four pediatric neurology departments in Turkey were enrolled in the study: 37 with limb-girdle muscle weakness and 35 with nonspecific hyperCKemia. Acid α-glucosidase (GAA) activity was measured on DBS by tandem mass spectrometry. Six patients tested positively for Pompe disease. In three patients, one with the limb-girdle muscle weakness and two with nonspecific hyperCKemia, this was confirmed by genetic analysis...
September 6, 2016: Neuromuscular Disorders: NMD
Jiewei Deng, Wenwen Wang, Yunyun Yang, Xiaowei Wang, Baowei Chen, Zhong-Ping Yao, Tiangang Luan
Analysis of trace compounds in small-volume complex samples is of importance for forensic, clinical, pharmaceutical, environmental, and life science investigation. In this study, we reported the coupling of slug-flow microextraction with paper spray mass spectrometry for rapid analysis of trace analytes in small volume of complicated biological samples such as whole blood, milk, and body fluid, etc. The method is performed by applying a disposable glass capillary for rapid extraction of a small amount of complex samples using a small amount of organic solvent; the loaded organic solvent was then spotted onto a paper triangle and dried out; subsequently, a high voltage and some spray solvent were applied onto the paper triangle for mass spectrometric analysis...
October 12, 2016: Analytica Chimica Acta
Andrea I Zambrano, Shekhar Sharma, Kathryn Crowley, Laura Dize, Beatriz E Muñoz, Sailesh K Mishra, Lisa A Rotondo, Charlotte A Gaydos, Sheila K West
BACKGROUND: The World Health Organization (WHO) now requires a second surveillance survey for trachoma after an impact assessment has found follicular trachoma (TF) <5% to determine if re-emergence has occurred. Using new WHO guidelines, we undertook surveillance surveys, and determined the prevalence of infection and antibody positivity, in two districts in Nepal. METHODS: 20 clusters were randomly selected within each district, 15 were randomly selected for antibody testing...
September 2016: PLoS Neglected Tropical Diseases
Ferdos Nazari, Farnaz Sinaei, Yalda Nilipour, Farzad Fatehi, Berthold Streubel, Mahmoud Reza Ashrafi, Omid Aryani, Shahriar Nafissi
INTRODUCTION: Pompe disease is characterized by absence or deficiency of acid alpha glucosidase, and several causative mutations are known. In this study we report clinical and lab data in Iranian patients with late-onset Pompe disease (LOPD), focusing on population-specific mutations. METHODS: Clinical and laboratory data of 14 patients from 10 families with the diagnosis of LOPD were recorded. All had reduced enzyme activity on dried blood spot (DBS) analysis...
September 20, 2016: Muscle & Nerve
Carlos A Saavedra-Matiz, Paola Luzi, Matthew Nichols, Joseph J Orsini, Michele Caggana, David A Wenger
Newborn screening (NBS) for Krabbe's disease (KD) has been instituted in several states, and New York State has had the longest experience. After an initial screening of dried blood spots, samples from individuals with galactocerebrosidase (GALC) values below a given cutoff level were subjected to additional testing, including sequencing of the GALC gene. This resulted in the identification of mutations that had previously been found in confirmed KD patients and of variants that had never previously been reported...
November 2016: Journal of Neuroscience Research
Alexandra V Ricca, Eric W Hall, Christine M Khosropour, Patrick S Sullivan
BACKGROUND: In the United States, men who have sex with men (MSM) are known to disproportionately have HIV. The authors sought to describe the acceptability of providing at-home dried blood spot specimen collection kits for HIV testing among MSM. METHODS: Between August 2010 and December 2010, the authors recruited Internet-using, HIV-negative or -unknown MSM to participate in a 12-month study of behavioral risks. Eligible participants were mailed an at-home HIV test...
September 15, 2016: Journal of the International Association of Providers of AIDS Care
Shin-Ichiro Hagiwara, Mitsuru Kubota, Ryusuke Nambu, Seiichi Kagimoto
BACKGROUND: It is important to assess pediatric patients for nutritional deficiencies when they are receiving specific interventions, such as enteral feeding. We focused our attention on measuring C0 and C5-OH with tandem mass spectrometry (MS/MS), which is performed as part of the newborn mass screening. The purpose of this study is to investigate the usefulness of MS/MS for screening carnitine and biotin deficiencies. METHODS: Forty-two children (24 males and 18 females) were enrolled between December 2013 and December 2015...
September 16, 2016: Pediatrics International: Official Journal of the Japan Pediatric Society
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