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dried blood spot

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https://www.readbyqxmd.com/read/28220711/comparison-of-hba1c-detection-in-whole-blood-and-dried-blood-spots-using-an-automated-ion-exchange-hplc-system
#1
Aleksandra Maleska, Christophe Hirtz, Elise Casteleyn, Orianne Villard, Jacques Ducos, Antoine Avignon, Ariane Sultan, Sylvain Lehmann
AIM: Hemoglobin A1c (HbA1c) is a widely recognized analyte for diagnosing and monitoring diabetes. Dried blood spot (DBS) constitutes a useful alternative to blood collection by venipuncture. Analytical and clinical validation of DBS use is, however, necessary before implementation. Results/methodology: HbA1c levels from whole blood or DBS from a cohort patients with diabetes were compared. DBS specimens were stable at ambient temperature. HbA1c detection on DBS was accurate, robust, and the correlation and agreement with whole blood values was excellent...
March 2017: Bioanalysis
https://www.readbyqxmd.com/read/28215086/salt-segregation-and-sample-cleanup-on-perfluoro-coated-nanostructured-surfaces-for-laser-desorption-ionization-mass-spectrometry-of-biofluid-samples
#2
Ya Zhou, Chen Peng, Kenneth D Harris, Rupasri Mandal, D Jed Harrison
We present a surface assisted laser desorption ionization (SALDI) technique, coupled with fluorocarbon coating, to achieve selective segregation of ionic and/or hydrophilic analytes from background biofluid electrolytes for quantiatve mass spectrometric analysis. By controlling the contact angle of (1H,1H,2H,2H-perfluorooctyl) trichlorosilane or (1H,1H,2H,2H-perfluorooctyl) dimethylchlorosilane to a specific range (105-120°), background electrolytes can be made to segregate from hydrophilic analytes during a drying step on the surface of a highly nanoporous thin film...
February 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28212681/toxoplasma-gondii-survey-in-cats-from-two-environments-of-the-city-of-rio-de-janeiro-brazil-by-modified-agglutination-test-on-sera-and-filter-paper
#3
Paula F Bolais, Philippe Vignoles, Pamela F Pereira, Rafael Keim, Abdelkrim Aroussi, Khadja Ismail, Marie-Laure Dardé, Maria Regina Amendoeira, Aurélien Mercier
BACKGROUND: Toxoplasma gondii is a protozoan with a worldwide distribution, in warm-blood animals, including humans. Local conditions and environmental disturbances may influence transmission dynamics of a zoonotic agent. This study evaluates the epidemiology of T. gondii based on toxoplasmosis prevalence in two populations of cats living in distinct urbanization conditions in Rio de Janeiro, Brazil. METHODS: Among 372 domestic cats sampled, 265 were from a public shelter located downtown Rio and 107 from a relatively preserved wild environment in a residential area...
February 17, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28212643/vertical-hiv-transmission-in-perinatally-exposed-infants-in-south-rift-region-of-kenya-a-retrospective-cross-sectional-study
#4
Everline Ashiono, Dunstan Achwoka, Jamlick Mutugi, Joel Rakwar, Andrew Wafula, Otto Nzapfurundi Chabikuli
BACKGROUND: Despite proven efficacy of the prevention mother-to-child transmission of HIV strategy, its adoption in Africa has remained slow. In Kenya, its effectiveness remain unknown. The aim of this study was to assess the effectiveness of a prevention of mother-to-child transmission program in Kenya. METHODS: This retrospective cross-sectional study analyzed 2,642 records of HIV-exposed infants who had a deoxyribonucleic acid polymerase chain reaction test done...
February 17, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28190990/the-translation-of-lipid-profiles-to-nutritional-biomarkers-in-the-study-of-infant-metabolism
#5
Animesh Acharjee, Philippa Prentice, Carlo Acerini, James Smith, Ieuan A Hughes, Ken Ong, Julian L Griffin, David Dunger, Albert Koulman
INTRODUCTION: Links between early life exposures and later health outcomes may, in part, be due to nutritional programming in infancy. This hypothesis is supported by observed long-term benefits associated with breastfeeding, such as better cognitive development in childhood, and lower risks of obesity and high blood pressure in later life. However, the possible underlying mechanisms are expected to be complex and may be difficult to disentangle due to the lack of understanding of the metabolic processes that differentiate breastfed infants compared to those receiving just formula feed...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/28183031/threat-related-amygdala-activity-is-associated-with-peripheral-crp-concentrations-in-men-but-not-women
#6
Johnna R Swartz, Aric A Prather, Ahmad R Hariri
Increased levels of peripheral inflammatory markers, including C-Reactive Protein (CRP), are associated with increased risk for depression, anxiety, and suicidality. The brain mechanisms that may underlie the association between peripheral inflammation and internalizing problems remain to be determined. The present study examines associations between peripheral CRP concentrations and threat-related amygdala activity, a neural biomarker of depression and anxiety risk, in a sample of 172 young adult undergraduate students...
January 25, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28178597/determination-of-topiramate-in-dried-blood-spots-using-single-quadrupole-gas-chromatography-mass-spectrometry-after-flash-methylation-with-trimethylanilinium-hydroxide
#7
Roberta Zilles Hahn, Marina Venzon Antunes, Priscila Costa Arnhold, Natalia Bordin Andriguetti, Simone Gasparin Verza, Rafael Linden
Dried blood spots (DBS) sampling obtained from fingerpricks is a promising and patient friendly alternative for obtaining samples for drug quantification, that could be of interest for topiramate (TOP) therapeutic drug monitoring. The aim of this study was to develop and validate a simple and fast GC-MS assay for TOP measurement in dried blood spots (DBS). The method uses a liquid extraction of one 8mm DBS, followed by a flash methylation with TMAH, and separation in a DB-5ms capillary column. Total analytical run time was 15min...
February 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28174312/selective-whole-genome-amplification-is-a-robust-method-that-enables-scalable-whole-genome-sequencing-of-plasmodium-vivax-from-unprocessed-clinical-samples
#8
Annie N Cowell, Dorothy E Loy, Sesh A Sundararaman, Hugo Valdivia, Kathleen Fisch, Andres G Lescano, G Christian Baldeviano, Salomon Durand, Vince Gerbasi, Colin J Sutherland, Debbie Nolder, Joseph M Vinetz, Beatrice H Hahn, Elizabeth A Winzeler
: Whole-genome sequencing (WGS) of microbial pathogens from clinical samples is a highly sensitive tool used to gain a deeper understanding of the biology, epidemiology, and drug resistance mechanisms of many infections. However, WGS of organisms which exhibit low densities in their hosts is challenging due to high levels of host genomic DNA (gDNA), which leads to very low coverage of the microbial genome. WGS of Plasmodium vivax, the most widely distributed form of malaria, is especially difficult because of low parasite densities and the lack of an ex vivo culture system...
February 7, 2017: MBio
https://www.readbyqxmd.com/read/28172740/p686-application-of-dried-blood-spots-for-pharmacokinetic-profiling-of-golimumab-treated-patients-with-ulcerative-colitis
#9
I Detrez, V Ballet, G Van Assche, S Vermeire, M Ferrante, A Gils
No abstract text is available yet for this article.
February 1, 2017: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/28168002/hepatitis-c-virus-seroprevalence-in-the-general-female-population-of-9-countries-in-europe-asia-and-africa
#10
Gary M Clifford, Tim Waterboer, Bolormaa Dondog, You Lin Qiao, Dimitri Kordzaia, Doudja Hammouda, Namory Keita, Nahid Khodakarami, Syed Ahsan Raza, Ang Tshering Sherpa, Witold Zatonski, Michael Pawlita, Martyn Plummer, Silvia Franceschi
BACKGROUND: New oral treatments with very high cure rates have the potential to revolutionize global management of hepatitis C virus (HCV), but population-based data on HCV infection are missing in many low and middle-income countries (LMIC). METHODS: Between 2004 and 2009, dried blood spots were collected from age-stratified female population samples of 9 countries: China, Mongolia, Poland, Guinea, Nepal, Pakistan, Algeria, Georgia and Iran. HCV antibodies were detected by a multiplex serology assay using bead-based technology...
2017: Infectious Agents and Cancer
https://www.readbyqxmd.com/read/28166235/development-and-clinical-performance-of-high-throughput-loop-mediated-isothermal-amplification-for-detection-of-malaria
#11
Rushini S Perera, Xavier C Ding, Frank Tully, James Oliver, Nigel Bright, David Bell, Peter L Chiodini, Iveth J Gonzalez, Spencer D Polley
BACKGROUND: Accurate and efficient detection of sub-microscopic malaria infections is crucial for enabling rapid treatment and interruption of transmission. Commercially available malaria LAMP kits have excellent diagnostic performance, though throughput is limited by the need to prepare samples individually. Here, we evaluate the clinical performance of a newly developed high throughput (HTP) sample processing system for use in conjunction with the Eiken malaria LAMP kit. METHODS: The HTP system utilised dried blood spots (DBS) and liquid whole blood (WB), with parallel sample processing of 94 samples per run...
2017: PloS One
https://www.readbyqxmd.com/read/28165155/performance-of-anti-hcv-testing-in-dried-blood-spots-and-saliva-according-to-hiv-status
#12
Geane Lopes Flores, Helena Medina Cruz, Vanessa Alves Marques, Cristiane Alves Villela-Nogueira, Denise Vigo Potsch, Silvia Beatriz May, Carlos Eduardo Brandão-Mello, Marcia Maria Amendola Pires, Jose Henrique Pilotto, Priscila Pollo-Flores, Eliane Bordalo Cathalá Esberard, Claudia Ivantes, Lia Laura Lewis-Ximenez, Elisabeth Lampe, Livia Melo Villar
The use of saliva and dried blood spots (DBS) could increase access to HCV diagnosis for high-risk populations, such as HIV-infected individuals, but the performance of these assays has not been well established in this group. This study aims to evaluate HIV status, particularly TCD4(+) cell count and viral load, in the performance of anti-HCV testing using DBS and saliva. A total of 961 individuals classified as HCV + , HIV+ or HIV/HCV + , as well as negative controls, donated serum, DBS and saliva samples for anti-HCV testing using a commercial enzyme immunoassay...
February 6, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28157384/evaluation-of-the-analytical-and-clinical-concordance-of-25-hydroxyvitamin-d-levels-in-dried-blood-spots-dried-serum-spots-and-serum-as-potential-biorepository-specimens
#13
Gellert Karvaly, Györgyi Molnár-Világos, Krisztián Kovács, Katalin Mészáros, Attila Patócs, Barna Vásárhelyi
CONTEXT: The promising perspective of storing dried blood spot (DBS) for evaluating 25-hydroxyvitamin D (25OHD) levels is increasingly being realized. While strong correlations have been demonstrated between 25OHD levels measured in DBS and in systemic serum samples in earlier works, the clinical concordance of the assay results has not been evaluated. Moreover, the utility of dried serum spot (DSS), a highly suitable matrix for sample archiving, has not been investigated in this respect...
February 3, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28153527/anemia-prevalence-and-hemoglobin-levels-are-associated-with-long-term-exposure-to-air-pollution-in-an-older-population
#14
Trenton Honda, Vivian C Pun, Justin Manjourides, Helen Suh
BACKGROUND: Anemia, a highly prevalent disorder in elderly populations, is associated with numerous adverse health outcomes, including increased mortality, impaired functional status and cognitive disorders. Approximately two-thirds of anemia in American elderly is caused by chronic inflammation or is unexplained. A potential contributing factor may include air pollution exposures, which have been shown to increase systemic inflammation and affect erythropoiesis. Few studies, however, have investigated the associations of air pollution on hemoglobin levels and anemia...
January 30, 2017: Environment International
https://www.readbyqxmd.com/read/28153274/quantification-of-the-3%C3%AE-and-3%C3%AE-epimers-of-25-hydroxyvitamin-d3-in-dried-blood-spots-by-lc-ms-ms-using-artificial-whole-blood-calibration-and-chemical-derivatization
#15
Miriam J Müller, Caroline S Stokes, Dietrich A Volmer
While the biological function of the 3α epimer of 25-hydroxyvitamin D3 (25(OH)D3) remains unknown, its presence needs to be accurately captured and separated from the main 3β epimer, to avoid positive bias in vitamin D status analyses. Several recent LC-MS/MS assays for 25(OH)D3 successfully separate the 3α and 3β epimers by chromatography. Unfortunately, none of the existing LC-MS/MS assays, which utilize dried blood spots (DBS) as sampling/storage vessels, is able to quantify the individual epimers. DBS are often used for analysis of infant blood, however, and these samples are particularly likely to contain significant levels of interfering 3α epimer...
April 1, 2017: Talanta
https://www.readbyqxmd.com/read/28151899/cohort-study-on-maternal-cytomegalovirus-seroprevalence-and-prevalence-and-clinical-manifestations-of-congenital-infection-in-china
#16
Shiwen Wang, Tongzhan Wang, Wenqiang Zhang, Xiaolin Liu, Xiaofang Wang, Haiyan Wang, Xiaozhou He, Shunxian Zhang, Shuhui Xu, Yang Yu, Xingbing Jia, Maolin Wang, Aiqiang Xu, Wei Ma, Minal M Amin, Stephanie R Bialek, Sheila C Dollard, Chengbin Wang
Congenital cytomegalovirus (CMV) infection is the leading viral cause of birth defects and developmental disabilities in developed countries. However, CMV seroprevalence and burden of congenital CMV infection are not well defined in China.Cohort of newborns from 5 birthing hospitals in 2 counties of Shandong Province, China, were enrolled from March 2011 to August 2013. Dried blood spots (DBS) and saliva were collected within 4 days after birth for IgG testing for maternal seroprevalence and real-time PCR testing for congenital CMV infection, respectively...
February 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28149263/advantages-and-challenges-of-dried-blood-spot-analysis-by-mass-spectrometry-across-the-total-testing-process
#17
Rosita Zakaria, Katrina J Allen, Jennifer J Koplin, Peter Roche, Ronda F Greaves
INTRODUCTION: Through the introduction of advanced analytical techniques and improved throughput, the scope of dried blood spot testing utilising mass spectrometric methods, has broadly expanded. Clinicians and researchers have become very enthusiastic about the potential applications of dried blood spot based mass spectrometric applications. Analysts on the other hand face challenges of sensitivity, reproducibility and overall accuracy of dried blood spot quantification. In this review, we aim to bring together these two facets to discuss the advantages and current challenges of non-newborn screening applications of dried blood spot quantification by mass spectrometry...
December 2016: EJIFCC
https://www.readbyqxmd.com/read/28146591/high-levels-of-transmitted-hiv-drug-resistance-in-a-study-in-papua-new-guinea
#18
Evelyn Lavu, Ellan Kave, Euodia Mosoro, Jessica Markby, Eman Aleksic, Janet Gare, Imogen A Elsum, Gideon Nano, Petronia Kaima, Nick Dala, Anup Gurung, Silvia Bertagnolio, Suzanne M Crowe, Mark Myatt, Anna C Hearps, Michael R Jordan
INTRODUCTION: Papua New Guinea is a Pacific Island nation of 7.3 million people with an estimated HIV prevalence of 0.8%. ART initiation and monitoring are guided by clinical staging and CD4 cell counts, when available. Little is known about levels of transmitted HIV drug resistance in recently infected individuals in Papua New Guinea. METHODS: Surveillance of transmitted HIV drug resistance in a total of 123 individuals recently infected with HIV and aged less than 30 years was implemented in Port Moresby (n = 62) and Mount Hagen (n = 61) during the period May 2013-April 2014...
2017: PloS One
https://www.readbyqxmd.com/read/28143689/reversible-brain-atrophy-in-glutaric-aciduria-type-1
#19
Yurika Numata-Uematsu, Osamu Sakamoto, Yosuke Kakisaka, Yukimune Okubo, Yoshitsugu Oikawa, Natsuko Arai-Ichinoi, Shigeo Kure, Mitsugu Uematsu
Glutaric aciduria type 1 (GA1) is a rare metabolic disorder caused by a deficiency of glutaryl-CoA dehydrogenase. The typical clinical onset features an acute encephalopathic crisis developed in early childhood, causing irreversible striatal injury. Recently, tandem mass spectrometry of spots of dried blood has allowed pre-symptomatic detection of GA1 in newborns. Early treatment can prevent irreversible neurological injury. We report the case of a girl with GA1 who exhibited a characteristic reversible change upon brain magnetic resonance imaging (MRI)...
January 28, 2017: Brain & Development
https://www.readbyqxmd.com/read/28143602/rubella-in-sub-saharan-africa-and-sensorineural-hearing-loss-a-case-control-study
#20
Cristina Caroça, Vera Vicente, Paula Campelo, Maria Chasqueira, Helena Caria, Susana Silva, Paulo Paixão, João Paço
BACKGROUND: Rubella infection can affect several organs and cause birth defects that are responsible for congenital rubella syndrome (CRS). Congenital hearing loss is the most common symptom of this syndrome, occurring in approximately 60% of CRS cases. Worldwide, over 100 000 babies are born with CRS every year. There is no specific treatment for rubella, but the disease is preventable by vaccination. Since 1969, the rubella vaccine has been implemented in many countries, but in Africa, only a few countries routinely immunize against rubella...
February 1, 2017: BMC Public Health
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