Wen Hu, Careen Foord, Justine Hsu, Li Fan, Michael J Corley, Tarun N Bhatia, Siwei Xu, Natan Belchikov, Yi He, Alina Ps Pang, Samantha N Lanjewar, Julien Jarroux, Anoushka Joglekar, Teresa A Milner, Lishomwa C Ndhlovu, Jing Zhang, Eduardo Butelman, Steven A Sloan, Virginia My Lee, Li Gan, Hagen U Tilgner
Multimodal measurements have become widespread in genomics, however measuring open chromatin accessibility and splicing simultaneously in frozen brain tissues remains unconquered. Hence, we devised Single-Cell-ISOform-RNA sequencing coupled with the Assay-for-Transposase-Accessible-Chromatin (ScISOr-ATAC). We utilized ScISOr-ATAC to assess whether chromatin and splicing alterations in the brain convergently affect the same cell types or divergently different ones. We applied ScISOr-ATAC to three major conditions: comparing (i) the Rhesus macaque ( Macaca mulatta ) prefrontal cortex (PFC) and visual cortex (VIS), (ii) cross species divergence of Rhesus macaque versus human PFC, as well as (iii) dysregulation in Alzheimer's disease in human PFC...
February 28, 2024: bioRxiv