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https://www.readbyqxmd.com/read/28109197/everolimus-is-better-than-rapamycin-in-attenuating-neuroinflammation-in-kainic-acid-induced-seizures
#1
Ming-Tao Yang, Yi-Chin Lin, Whae-Hong Ho, Chao-Lin Liu, Wang-Tso Lee
BACKGROUND: Microglia is responsible for neuroinflammation, which may aggravate brain injury in diseases like epilepsy. Mammalian target of rapamycin (mTOR) kinase is related to microglial activation with subsequent neuroinflammation. In the present study, rapamycin and everolimus, both as mTOR inhibitors, were investigated in models of kainic acid (KA)-induced seizure and lipopolysaccharide (LPS)-induced neuroinflammation. METHODS: In vitro, we treated BV2 cells with KA and LPS...
January 21, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28109194/family-health-strategy-and-equity-in-prenatal-care-a-population-based-cross-sectional-study-in-minas-gerais-brazil
#2
Mônica Viegas Andrade, Kenya Valéria Micaela de Souza Noronha, Allan Claudius Queiroz Barbosa, Michelle Nepomuceno Souza, Júlia Almeida Calazans, Lucas Resende de Carvalho, Thiago Augusto Hernandes Rocha, Núbia Cristina Silva
BACKGROUND: Prenatal care coverage is still not universal or adequately provided in many low and middle income countries. One of the main barriers regards the presence of socioeconomic inequalities in prenatal care utilization. In Brazil, prenatal care is supplied for the entire population at the community level as part of the Family Health Strategy (FHS), which is the main source of primary care provided by the public health system. Brazil has some of the greatest income inequalities in the world, and little research has been conducted to investigate prenatal care utilization of FHS across socioeconomic groups...
January 21, 2017: International Journal for Equity in Health
https://www.readbyqxmd.com/read/28109190/qtl-mapping-and-candidate-genes-for-resistance-to-fusarium-ear-rot-and-fumonisin-contamination-in-maize
#3
Valentina Maschietto, Cinzia Colombi, Raul Pirona, Giorgio Pea, Francesco Strozzi, Adriano Marocco, Laura Rossini, Alessandra Lanubile
BACKGROUND: Fusarium verticillioides is a common maize pathogen causing ear rot (FER) and contamination of the grains with the fumonisin B1 (FB1) mycotoxin. Resistance to FER and FB1 contamination are quantitative traits, affected by environmental conditions, and completely resistant maize genotypes to the pathogen are so far unknown. In order to uncover genomic regions associated to reduced FER and FB1 contamination and identify molecular markers for assisted selection, an F2:3 population of 188 progenies was developed crossing CO441 (resistant) and CO354 (susceptible) genotypes...
January 21, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28109184/comparative-cardiovascular-risks-of-dipeptidyl-peptidase-4-inhibitors-with-other-2-nd-and-3-rd-line-antidiabetic-drugs-in-patients-with-type-2-diabetes
#4
Huang-Tz Ou, Kai-Cheng Chang, Chung-Yi Li, Jin-Shang Wu
AIMS: Dipeptidyl peptidase-4 inhibitors (DPP4i) are suggested as 2(nd) and 3(rd) add-on antidiabetic treatment for type 2 diabetes. Previous studies only assessed cardiovascular effects of DPP4i as 2(nd) line treatment, included sulfonylurea as only one comparator, and yielded inconclusive results on the risk of heart failure. This study therefore evaluated comparative cardiovascular risks of DPP4i with other 2(nd) and 3(rd) line antidiabetic drugs. METHODS: Based on a large nation-wide diabetic cohort, 113,051 patients with type 2 diabetes newly on metformin-based dual or triple therapy were identified in 2009-2011 and followed until death or 2013...
January 21, 2017: British Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/28109181/stability-of-alcohol-use-and-teen-dating-violence-for-female-youth-a-latent-transition-analysis
#5
Hye Jeong Choi, JoAnna Elmquist, Ryan C Shorey, Emily F Rothman, Gregory L Stuart, Jeff R Temple
INTRODUCTION AND AIMS: Alcohol use is one of the most widely accepted and studied risk factors for teen dating violence (TDV). Too little research has explored longitudinally if it is true that an adolescent's alcohol use and TDV involvement simultaneously occur. In the current study, we examined whether there were latent status based on past-year TDV and alcohol use and whether female adolescents changed their statuses of TDV and alcohol use over time. METHODS: The sample consisted of 583 female youths in seven public high schools in Texas...
January 21, 2017: Drug and Alcohol Review
https://www.readbyqxmd.com/read/28109179/quantitative-and-integrative-analysis-of-paracrine-hepatocyte-activation-by-non-parenchymal-cells-upon-lipopolysaccharide-induction
#6
Katharina Beuke, Frank A Schildberg, Federico Pinna, Ute Albrecht, Roman Liebe, Michaela Bissinger, Peter Schirmacher, Steven Dooley, Johannes G Bode, Percy A Knolle, Ursula Kummer, Kai Breuhahn, Sven Sahle
Gut-derived bacterial lipopolysaccharides (LPS) stimulate the secretion of tumor necrosis factor (TNF) from liver macrophages (MCs), liver sinusoidal endothelial cells (LSECs), and hepatic stellate cells (HSCs), which control the acute phase response in hepatocytes through activation of the NF-κB pathway. The individual and cooperative impact of non-parenchymal cells on this clinically relevant response has not been analysed in detail due to technical limitations. To gain an integrative view on this complex inter- and intracellular communication, we combined a multi-scale mathematical model with quantitative, time-resolved experimental data of different primary murine liver cell types...
January 21, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28109167/cell-cycle-tailored-targeting-of-metastatic-melanoma-challenges-opportunities
#7
Nikolas K Haass, Brian Gabrielli
The advent of targeted therapies of metastatic melanoma, such as MAPK pathway inhibitors and immune checkpoint antagonists, has turned dermato-oncology from the 'bad guy' to the 'poster child' in oncology. Current targeted therapies are effective; although here is a clear need to develop combination therapies to delay the onset of resistance. Many anti-melanoma drugs impact on the cell cycle but are also dependent on certain cell cycle phases resulting in cell cycle phase-specific drug insensitivity. Here, we raise the question: Have combination trials been abandoned prematurely as ineffective possibly only because drug scheduling was not optimized? Firstly, if both drugs of a combination hit targets in the same melanoma cell, cell cycle-mediated drug insensitivity should be taken into account when planning combination therapies, timing of dosing schedules and choice of drug therapies in solid tumors...
January 21, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28109163/an-assessment-of-the-overexpression-of-bmp-2-in-transfected-human-osteoblast-cells-stimulated-by-mta-and-biodentine
#8
E M Rodrigues, A L G Cornélio, A S C Fuentes, L P Salles, M Velayutham, C Rossa-Junior, J M Guerreiro-Tanomaru, M Tanomaru-Filho
AIM: To evaluate the effect of MTA and Biodentine on viability, osteogenic differentiation and BMP-2 expression in osteogenic cells. METHODOLOGY: Saos-2 cells were used as a model of osteoblastic cells. Overexpression of BMP2 was induced by transfection of a CMV-driven plasmid construct including the human BMP-2 coding sequence and stably transfected cells were selected. Cell viability was assessed by the mitochondrial dehydrogenase enzymatic (MTT) assay. The bioactivity of the materials was evaluated by the Alkaline Phosphatase (ALP) assay and detection of calcium deposits with Alizarin Red Staining (ARS)...
January 21, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28109160/antibiotics-for-infection-prevention-after-excision-of-the-cervical-transformation-zone
#9
REVIEW
Chumnan Kietpeerakool, Bandit Chumworathayi, Jadsada Thinkhamrop, Butsakorn Ussahgij, Pisake Lumbiganon
BACKGROUND: Excision of the transformation zone of the cervix is the most commonly used approach to treat cervical precancerous lesions (cervical intraepithelial neoplasia (CIN)) to reduce the risk of developing cervical cancer. As the excision of the transformation zone leaves a raw area on the cervix, there is a risk of infection following the procedure. The incidence of infection after cold knife conization (CKC) is 36%, whereas the incidence for large loop excision of the transformation zone (LLETZ, also known as loop electrical excision procedure (LEEP)) is much lower (0...
January 21, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28109154/-inhibitory-effect-of-overexpressed-kisspeptin-on-proliferation-and-migration-of-mkn-45-gastric-cancer-cells
#10
Xin Wang, Yijiong Li, Ruihua Zhang, Chao Pang, Xiaoqing Jiao, Zhenlong Zhu, Zhengmin Wang
Objective To investigate the expression of kisspeptin in gastric adenocarcinoma and its effect on the proliferation and migration of gastric cancer cells. Methods The level of kisspeptin in 50 gastric cancer tissues and their paracancerous tissues were detected by quantitative real-time PCR and Western blotting. Kisspeptin-siRNA, control-siRNA, pEGFP-N1-kisspeptin, pEGFP-N1 were separately transfected into MKN-45 gastric cancer cells. After 48-hour culture, the levels of matrix metalloproteinase-2 (MMP-2), β-catenin, C-myc, MMP-9, kisspeptin were detected by Western blotting; MTT assay was used to detect the proliferation of MKN-45 cells; wound healing assay was performed to assess the migration ability of MKN-45 cells...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109153/-chemotherapy-increases-fc%C3%AE-riib-content-in-peripheral-blood-of-the-patients-with-lung-squamous-cell-carcinoma
#11
Jingjing Lu, Xiuqin Cao, Zhiwei Yang
Objective To detect the FcγRIIB content of monocytes and B cells, the concentration of soluble FcγRIIB (sFcγRIIB) and its antibodies in the peripheral blood of patients with lung squamous cell carcinoma (LSCC) before and after chemotherapy. Methods The expression and localization of FcγRIIB on monocytes and B cells were detected by immunofluorescence technique; the expression of FcγRIIB mRNA was examined by quantitative real-time PCR; Western blotting was performed to detect the expression of FcγRIIB protein; the serum concentrations of sFcγRIIB, sFcγRIIB-IgG complex, anti-FcγRIIB autoantibody were determined by ELISA...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109152/-hepatic-stellate-cell-conditioned-medium-induces-proliferation-and-epithelial-mesenchymal-transition-via-activating-erk1-2-signaling-pathway-in-hepatoma-cells
#12
Yuxiao Xie, Rui Liao, Long Pan, Kai Fan, Cong Peng, Chengyou Du
Objective To investigate the influence of hepatic stellate cells (HSCs) on malignant biological behavior of hepatoma cells and related mechanisms. Methods Human hepatoma cell lines SMMC-7721 and HepG2, and hepatic stellate cell line LX-2 were cultured separately. HSC conditioned medium (LX2-CM), MAPK specific inhibitor U0126 were used to treat hepatoma cells, separately or together. The invasion and migration abilities of hepatoma cells were detected by Transwell(TM) assay, and cell proliferation was analyzed by CCK-8 assay...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109151/-tacrolimus-down-regulates-the-mrna-levels-of-il-17-and-il-23-in-the-muscle-tissues-of-mice-with-experimental-autoimmune-myositis
#13
Xiaojia Tian, Fangyuan He, Hongge Jia, Hongya Zhang
Objective To measure the expression levels of interleukin-17 (IL-17) and IL-23 mRNAs in the muscle tissue of the mice with experimental autoimmune myositis (EAM), and investigate the impact of tacrolimus (TAC) treatment on the mRNA levels of IL-17 and IL-23 and its therapeutic effect in EAM mice. Methods Fifteen female BALB/c mice were divided randomly into three groups, a normal control group, an EAM model group and a TAC-treated group. HE staining was used to observe the pathological changes for evaluating muscle inflammation of EAM mice...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109147/-co-culture-with-umbilical-cord-mesenchymal-stem-cells-promotes-the-synthesis-and-mechnism-of-milk-protein-in-bovine-mammary-epithelial-cells
#14
Yankun Zhao, Wei Shao, Chenglong Luo, Kaile Wu, Xiong Yu
Objective To explore the possible mechanism of umbilical cord mesenchymal stem cells (UCMSCs) regulating milk protein synthesis in bovine mammary gland epithelial cells (BMECs). Methods UCMSCs and BMECs were co-cultured by double-chamber Transwell(TM), and other BMECs were cultured alone as a control group. Insulin like growth factor 1 receptor (IGF-1R) inhibitor AG1024 was used to treat cells. IGF-1, β casein (CSN2) and κ casein (CSN3) content in the supernatants were determined by ELISA; the relative expression abundance of Janus kinase and signal transducer and activator of transcription factor (JAK/STAT) signaling pathway-related genes were detected by quantitative real-time PCR (qRT-PCR); and after the cells were treated with JAK2 signal blocker AG490, qRT-PCR was performed to test the relative expression abundance of CSN2 and CSN3 mRNAs...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109145/-short-term-use-of-rapamycin-combined-with-tregs-prolongs-mouse-cardiac-graft-survival-but-attenuates-recipient-s-anti-tumor-immunity
#15
Ting Yan, Chen Luo, Xianmo Yang, Lei Ji, Shiqiao Luo
Objective To study the effect of short-term use of rapamycin(Rap) combined with regulatory T cells (Tregs) on the long-time survival of allogeneic mouse cardiac transplant, and its impact on the anti-tumor immunity of recipient. MethodsMouse Tregs were purified from recipients' spleen by magnetic activated cell sorting (MACS), and expanded by CD3/CD28 monoclonal antibody immunomagnetic beads and 2000 U/mL recombinant mouse IL-2 (rmIL-2) ex vivo. The purity was tested by fluorescence-activated cell sorting (FACS)...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109144/-interferon-regulatory-factor-5-irf5-regulates-the-differentiation-of-bone-marrow-derived-macrophages-in-mice
#16
Kang Sun, Jianguo Qu, Jixiang Chen, Shengchun Dang, Songbing He, Jin Zhang, Rong Xie, Yun Wang, Jianxin Zhang
Objective To investigate the expression differences of interferon regulatory factor 5 (IRF5) between M1 and M2 macrophages derived from mouse bone marrow and the effects of small interfering RNA targeting IRF5 gene (IRF5 siRNA) on macrophage differentiation. Methods With the treatment of IFN-γ and lipopolysaccharide (LPS), mouse bone marrow-derived macrophages were differentiated into M1 macrophages; while with the treatment of IL-4, mouse bone marrow-derived macrophages were differentiated into M2 macrophages...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109143/-decreased-number-and-immune-activity-of-splenic-t-lymphocytes-in-mice-exposed-to-hypoxia-at-high-altitude
#17
Xiaona Zhang, Jidong Li, Fangjie Hu, Shilan Gao, Xiaoyan Pu, Sheng Yong
Objective To explore the changes of splenic T lymphocyte subsets and functions in mice under high-altitude hypoxic conditions. Methods After mice were exposed to an altitude of 400 m, 2200 m and 4200 m for 30 days, ELISA was used to detect the concentrations of interleukin-4 (IL-4) and interferon-γ (IFN-γ) in the cultured splenocyte supernatant; MTT assay was used to analyze the proliferation of splenic T lymphocytes; flow cytometry was performed to examine the alterations of splenic T lymphocyte subsets...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109142/-changes-and-role-evaluation-of-tnf-%C3%AE-and-il-1%C3%AE-in-lung-tissues-of-ards-mice
#18
Jianing Liang, Qianqian Zhou, Tianxiang Zhang, Xiaosu Wang, Liqiang Song
Objective To study the expression levels of TNF-α and IL-1β in the lung tissues of acute respiratory distress syndrome (ARDS) mice and their relationships with the severity of lung injury in the mice. Methods A mouse model of ARDS was induced by lipopolysaccharide (LPS). The morphological changes of lung tissue was observed by HE staining, and the lung injury score was calculated. Quantitative real-time PCR was employed to detect the mRNA expression levels of TNF-α and IL-1β in lung tissues and ELISA was performed to test the protein levels of TNF-α and IL-1β in bronchoalveolar lavage fluid (BALF)...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109141/-hydrogen-rich-saline-attenuates-hyperalgesia-and-reduces-cytokines-in-rats-with-post-herpetic-neuralgia-via-activating-autophagy
#19
Hongtao Ma, Hongguang Chen, Aili Dong, Yanyan Wang, Yingxue Bian, Keliang Xie
Objective To investigate the role of autophagy in hydrogen-rich saline attenuating post-herpetic neuralgia (PHN) in rats. Methods A total of 100 male SD rats were randomly divided into the five groups (n=20): control group, PHN group, PHN group treated with hydrogen-rich saline (PHN-H2 group), PHN group treated with hydrogen-rich saline and 3-MA (PHN-H2-3-MA group), PHN group treated with hydrogen-rich saline and rapamycin (PHN-H2-Rap group). PHN models were established by varicella-zoster virus (VZV) inoculation...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109131/changes-in-serum-creatinine-concentration-and-acute-kidney-injury-aki-grade-in-dogs-treated-with-hydroxyethyl-starch-130-0-4-from-2013-to-2015
#20
N E Sigrist, N Kälin, A Dreyfus
BACKGROUND: Hydroxyethyl starch (HES) solutions may cause acute kidney injury (AKI) in humans. OBJECTIVE: To compare AKI grades in 94 dogs exposed and 90 dogs that were unexposed to 6% HES-130/0.4. ANIMALS: Dogs receiving 6% HES-130/0.4 (HES cohort) or crystalloids (unexposed cohort) between 2013 and 2015. METHODS: Historical cohort study. Diagnosis, total cumulative dose and total mL/kg of HES administered, time frame of HES administration and serum creatinine concentrations up to 90 days after initiation of HES treatment were retrospectively reviewed...
January 21, 2017: Journal of Veterinary Internal Medicine
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