keyword
MENU ▼
Read by QxMD icon Read
search

KSHV

keyword
https://www.readbyqxmd.com/read/28212444/sumo-modification-of-a-heterochromatin-histone-demethylase-jmjd2a-enables-viral-gene-transactivation-and-viral-replication
#1
Wan-Shan Yang, Mel Campbell, Pei-Ching Chang
Small ubiquitin-like modifier (SUMO) modification of chromatin has profound effects on transcription regulation. By using Kaposi's sarcoma associated herpesvirus (KSHV) as a model, we recently demonstrated that epigenetic modification of viral chromatin by SUMO-2/3 is involved in regulating gene expression and viral reactivation. However, how this modification orchestrates transcription reprogramming through targeting histone modifying enzymes remains largely unknown. Here we show that JMJD2A, the first identified Jumonji C domain-containing histone demethylase, is the histone demethylase responsible for SUMO-2/3 enrichment on the KSHV genome during viral reactivation...
February 17, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28194991/simultaneous-detection-of-dual-nucleic-acids-using-a-sers-based-lateral-flow-assay-biosensor
#2
Xiaokun Wang, Namhyun Choi, Ziyi Cheng, Juhui Ko, Lingxin Chen, Jaebum Choo
A new class of surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) biosensor has been developed for the simultaneous detection of dual DNA markers. The LFA strip in this sensor was composed of two test lines and one control line. SERS nano tags labeled with detection DNA probes were used for quantitative evaluation of dual DNA markers with high sensitivity. Target DNA, associated with Kaposi's sarcoma-associated herpesvirus (KSHV) and bacillary angiomatosis (BA), were tested to validate the detection capability of this SERS-based LFA strip...
January 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28191343/molecular-epidemiology-of-kaposi-s-sarcoma-associated-herpes-virus-and-risk-factors-in-hiv-infected-patients-in-tehran-2014
#3
Khashayar Hesamizadeh, Hossein Keyvani, Farah Bokharaei-Salim, Seyed Hamidreza Monavari, Maryam Esghaei, Fatemeh Jahanbakhsh Sefidi
BACKGROUND: Kaposi's sarcoma (KS) remains the most common malignancy among HIV-infected patients. Human herpesvirus type-8 (HHV-8) is regarded as the infectious etiological agent of Kaposi's sarcoma (KSHV). Diagnostic procedures associated with KSHV are not routinely performed in HIV-infected subjects. OBJECTIVES: The main objective of this study is to obtain information on KSHV epidemiology in Iranian HIV-infected individuals. PATIENTS AND METHODS: In the present cross-sectional study, 109 patients with established HIV infection, who visited a governmental and referral center for HIV screening in Tehran (Tehran west health center (TWHC)) between May 2014 and July 2015 were enrolled according to the convenience sample strategy...
November 2016: Iranian Red Crescent Medical Journal
https://www.readbyqxmd.com/read/28165144/kaposi-s-sarcoma-associated-herpesvirus-kshv-encoded-micrornas-promote-matrix-metalloproteinases-mmps-expression-and-pro-angiogenic-cytokine-secretion-in-endothelial-cells
#4
Yuanyuan Guo, Wan Li, Jie Qin, Chun Lu, Weifei Fan
The human oncogenic virus Kaposi's sarcoma-associated herpesvirus (KSHV) is linked to Kaposi's sarcoma (KS), a tumor of endothelial cells characterized by angiogenesis and invasiveness. KSHV genome encodes 25 mature microRNAs (miRNAs), but their roles in KSHV-induced tumor dissemination and angiogenesis are not fully understood. In this study, we constructed the sensor reporters of KSHV miRNAs and used a luciferase reporter assay to demonstrate the function of the mimics of KSHV miRNAs. Then, we examined the expression of matrix metalloproteinases (MMPs) and pro-angiogenic cytokines that are related to cell migration and angiogenesis in the KSHV 25 miRNAs transfected endothelial cells...
February 6, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28161410/methylseleninic-acid-and-sodium-selenite-induce-severe-er-stress-and-subsequent-apoptosis-through-upr-activation-in-pel-cells
#5
Zenpei Shigemi, Kazuki Manabe, Naoko Hara, Yusuke Baba, Kohei Hosokawa, Hiroki Kagawa, Tadashi Watanabe, Masahiro Fujimuro
Selenium compounds such as methylseleninic acid (MSA) and sodium selenite (SS) have been widely evaluated as potential anti-cancer agents in the clinical setting. Primary effusion lymphoma (PEL) is a non-Hodgkin's B-cell lymphoma, associated with immunosuppressed individuals, such as post-transplant or AIDS patients. Kaposi's sarcoma-associated herpesvirus (KSHV) is the causative agent of PEL and Kaposi's sarcoma. Here, we found that MSA and SS markedly inhibited the growth of PEL cells compared with KSHV-uninfected B cells...
February 1, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28146424/up-regulation-of-tumor-suppressor-genes-by-exogenous-dhc16-cer-contributes-to-its-anti-cancer-activity-in-primary-effusion-lymphoma
#6
Yueyu Cao, Jing Qiao, Zhen Lin, Jovanny Zabaleta, Lu Dai, Zhiqiang Qin
Primary effusion lymphoma (PEL) is a rare and highly aggressive B-cell malignancy with Kaposi's sarcoma-associated herpesvirus (KSHV) infection, while lack of effective therapies. Our recent data indicated that targeting the sphingolipid metabolism by either sphingosine kinase inhibitor or exogenous ceramide species induces PEL cell apoptosis and suppresses tumor progression in vivo. However, the underlying mechanisms for these exogenous ceramides "killing" PEL cells remain largely unknown. Based on the microarray analysis, we found that exogenous dhC16-Cer treatment affected the expression of many cellular genes with important functions within PEL cells such as regulation of cell cycle, cell survival/proliferation, and apoptosis/anti-apoptosis...
January 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28132029/kshv-sox-mediated-host-shutoff-the-molecular-mechanism-underlying-mrna-transcript-processing
#7
Hyunah Lee, Anathe O M Patschull, Claire Bagnéris, Hannah Ryan, Christopher M Sanderson, Bahram Ebrahimi, Irene Nobeli, Tracey E Barrett
Onset of the lytic phase in the KSHV life cycle is accompanied by the rapid, global degradation of host (and viral) mRNA transcripts in a process termed host shutoff. Key to this destruction is the virally encoded alkaline exonuclease SOX. While SOX has been shown to possess an intrinsic RNase activity and a potential consensus sequence for endonucleolytic cleavage identified, the structures of the RNA substrates targeted remained unclear. Based on an analysis of three reported target transcripts, we were able to identify common structures and confirm that these are indeed degraded by SOX in vitro as well as predict the presence of such elements in the KSHV pre-microRNA transcript K12-2...
January 27, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28123439/sero-prevalence-of-bloodborne-tumor-viruses-hcv-hbv-htlv-i-and-kshv-infections-and-related-risk-factors-among-prisoners-in-razavi-khorasan-province-iran-in-2008
#8
Mohammad Khajedaluee, Ali Babaei, Rosita Vakili, Narges Valizade, Fateme Homaei Shandiz, Seyed Moayed Alavian, Mohsen Seyed Nozadi, Seyed Mohammad Jazayeri, Tahereh Hassannia
BACKGROUND: Prisoners are at high risk of blood borne and sexually transmitted infections due to their high involvement in risky behaviors. In this descriptive/cross-sectional study, the prevalence, sero-prevalence, and risk factors for bloodborne tumor viruses including HTLV-I, HBV, HCV, and KSHV were evaluated among inmates of two central prisons in the northeast of Iran. METHODS: Blood samples of 1114 inmates were analyzed for the presence of anti HTLV-I, KSHV, and HCV antibodies and HBsAg by ELISA...
December 2016: Hepatitis Monthly
https://www.readbyqxmd.com/read/28122468/kshv-associated-extracavitary-primary-effusion-lymphoma-in-an-hiv-seronegative-patient-a-case-report-and-review-of-the-literature
#9
Uroosa Ibrahim, Amina Saqib, Farhan Mohammad, Juan Ding, Shafinaz Hussein, Jean Paul Atallah
Primary effusion lymphoma (PEL) is a rare type of non-Hodgkin's lymphoma presenting as a lymphomatous effusion and absence of a solid tumor mass. Extracavitary PEL (EC-PEL) is a subtype of PEL with the absence of an effusion but presence of solid tumor. PEL and EC-PEL share the same histopathologic and immunophenotypic features. Kaposi sarcoma-associated herpesvirus (KSHV) positivity is seen universally in these malignancies and is a requisite for diagnosis. Most cases are seen to occur in HIV positive individuals...
January 26, 2017: Postgraduate Medicine
https://www.readbyqxmd.com/read/28118409/epigenetic-landscape-of-kaposi-s-sarcoma-associated-herpesvirus-genome-in-classic-kaposi-s-sarcoma-tissues
#10
Rui Sun, Xiaohua Tan, Xing Wang, Xiaodong Wang, Lei Yang, Erle S Robertson, Ke Lan
Kaposi's sarcoma-associated herpesvirus (KSHV) is etiologically related to Kaposi's sarcoma (KS), primary effusion lymphoma (PEL) and multicentric Castleman's disease (MCD). It typically displays two different phases in its life cycle, the default latency and occasional lytic replication. The epigenetic modifications are thought to determine the fate of KSHV infection. Previous studies elegantly depicted epigenetic landscape of latent viral genome in in vitro cell culture systems. However, the physiologically relevant scenario in clinical KS tissue samples is unclear...
January 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28107481/bet-inhibitors-disrupt-rad21-dependent-conformational-control-of-kshv-latency
#11
Horng-Shen Chen, Alessandra De Leo, Zhuo Wang, Andrew Kerekovic, Robert Hills, Paul M Lieberman
Kaposi's Sarcoma-associated Herpesvirus (KSHV) establishes stable latent infection in B-lymphocytes and pleural effusion lymphomas (PELs). During latency, the viral genome persists as an epigenetically constrained episome with restricted gene expression programs. To identify epigenetic regulators of KSHV latency, we screened a focused small molecule library containing known inhibitors of epigenetic factors. We identified JQ1, a Bromodomain and Extended Terminal (BET) protein inhibitor, as a potent activator of KSHV lytic reactivation from B-cells carrying episomal KSHV...
January 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28104704/perspective-the-promise-of-proteomics-in-the-study-of-oncogenic-viruses
#12
Alison Anne McBride
Oncogenic viruses are responsible for about 15% human cancers. This article explores the promise and challenges of viral proteomics in the study of the oncogenic human DNA viruses, HPV, McPyV, EBV and KSHV. These viruses have coevolved with their hosts and cause persistent infections. Each virus encodes oncoproteins that manipulate key cellular pathways to promote viral replication and evade the host immune response. Viral proteomics can identify cellular pathways perturbed by viral infection, identify cellular proteins that are crucial for viral persistence and oncogenesis, and identify important diagnostic and therapeutic targets...
January 19, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28102325/viral-micrornas-target-a-gene-network-inhibit-stat-activation-and-suppress-interferon-responses
#13
Dhivya Ramalingam, Joseph M Ziegelbauer
Kaposi's sarcoma-associated herpesvirus (KSHV) encodes 12 pre-microRNAs during latency that are processed to yield ~25 mature microRNAs (miRNAs). We were interested in identifying cellular networks that were targeted by KSHV-miRNAs and employed network building strategies using validated KSHV miRNA targets. Here, we report the identification of a gene network centering on the transcription factor- signal transducer and activator of transcription 3 (STAT3) that is targeted by KSHV miRNAs. KSHV miRNAs suppressed STAT3 and STAT5 activation and inhibited STAT3-dependent reporter activation upon IL6-treatment...
January 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28099521/nuclear-localization-and-cleavage-of-stat6-is-induced-by-kaposi-s-sarcoma-associated-herpesvirus-for-viral-latency
#14
Chong Wang, Caixia Zhu, Fang Wei, Shujun Gao, Liming Zhang, Yuhong Li, Yanling Feng, Yin Tong, Jianqing Xu, Bin Wang, Zhenghong Yuan, Erle S Robertson, Qiliang Cai
Emerging evidence implies that STAT6 plays an important role in both the adaptive and innate immune responses to virus infection. Kaposi's sarcoma-associated herpesvirus (KSHV) is an oncogenic γ-herpesvirus agent associated with several human malignancies, including Kaposi's sarcoma (KS) and primary effusion lymphomas (PELs). Previously, we demonstrated that KSHV blocks IL-4-induced STAT6 phosphorylation and retains a basal IL-13/STAT6 constitutive activation for cell survival and proliferation. However, the mechanism by which KSHV regulates STAT6 remains largely unknown...
January 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28092744/quercetin-induces-apoptosis-and-autophagy-in-primary-effusion-lymphoma-cells-by-inhibiting-pi3k-akt-mtor-and-stat3-signaling-pathways
#15
Marisa Granato, Celeste Rizzello, Maria Saveria Gilardini Montani, Laura Cuomo, Marina Vitillo, Roberta Santarelli, Roberta Gonnella, Gabriella D'Orazi, Alberto Faggioni, Mara Cirone
Quercetin, a bioflavonoid contained in several vegetables daily consumed, has been studied for long time for its antiinflammatory and anticancer properties. Quercetin interacts with multiple cancer-related pathways such as PI3K/AKT, Wnt/β-catenin and STAT3. These pathways are hyperactivated in primary effusion lymphoma (PEL), an aggressive B cell lymphoma whose pathogenesis is strictly linked to the oncogenic virus Kaposis' Sarcoma-associated Herpesvirus (KSHV). In this study, we found that quercetin inhibited PI3K/AKT/mTOR and STAT3 pathways in PEL cells, and as a consequence, it down-regulated the expression of the prosurvival cellular proteins such as c-FLIP, cyclin D1 and cMyc...
January 5, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28053098/the-viral-bcl-2-homologs-of-kaposi-s-sarcoma-associated-herpesvirus-and-rhesus-rhadinovirus-share-an-essential-role-for-viral-replication
#16
Antonio Gallo, Melanie Lampe, Thomas Günther, Wolfram Brune
: KS-Bcl-2 is a Kaposi's sarcoma-associated herpesvirus (KSHV)-encoded viral Bcl-2 homolog (vBcl-2), which has apoptosis and autophagy inhibiting activity when expressed in transfected cells. However, little is known about its function during viral infection. As KS-Bcl-2 is expressed during the lytic replication cycle, we used constitutively lytic and inducible-lytic KSHV mutants to investigate the role of KS-Bcl-2 during the lytic cycle. We show that KSHV cannot complete the lytic replication cycle and produce infectious progeny in the absence of KS-Bcl-2, indicating that the protein is essential for KSHV replication...
January 4, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28046107/rna-sequencing-reveals-that-kaposi-sarcoma-associated-herpesvirus-infection-mimics-hypoxia-gene-expression-signature
#17
Coralie Viollet, David A Davis, Shewit S Tekeste, Martin Reczko, Joseph M Ziegelbauer, Francesco Pezzella, Jiannis Ragoussis, Robert Yarchoan
Kaposi sarcoma-associated herpesvirus (KSHV) causes several tumors and hyperproliferative disorders. Hypoxia and hypoxia-inducible factors (HIFs) activate latent and lytic KSHV genes, and several KSHV proteins increase the cellular levels of HIF. Here, we used RNA sequencing, qRT-PCR, Taqman assays, and pathway analysis to explore the miRNA and mRNA response of uninfected and KSHV-infected cells to hypoxia, to compare this with the genetic changes seen in chronic latent KSHV infection, and to explore the degree to which hypoxia and KSHV infection interact in modulating mRNA and miRNA expression...
January 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28035362/mir-628-3p-regulates-osteoblast-differentiation-by-targeting-runx2-possible-role-in-atrophic-non-union
#18
Hua Chen, Xinran Ji, Fei She, Yuan Gao, Peifu Tang
Atrophic non-union is a serious complication of fractures. The underlying biological mechanisms involved in its pathogenesis are not yet completely understood. MicroRNAs (miRNAs or miRs) are a type of endogenous small non-coding RNA, which participate in various physiological and pathophysiological processes. In this study, differentially expressed miRNAs were screened in patients with atrophic non-union. In total, 4 miRNAs (miR‑149*, miR‑221, miR‑628-3p and miR‑654-5p) were upregulated and 7 miRNAs (let-7b*, miR‑220b, miR‑513a-3p, miR‑551a, miR‑576-5p, miR‑1236 and kshv-miR‑K12-6-5p) were downregulated at the fracture sites in patients with atrophic non-union...
February 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28004818/3-d-microwell-array-system-for-culturing-virus-infected-tumor-cells
#19
Rami El Assal, Umut A Gurkan, Pu Chen, Franceline Juillard, Alessandro Tocchio, Thiruppathiraja Chinnasamy, Chantal Beauchemin, Sebnem Unluisler, Serli Canikyan, Alyssa Holman, Srikar Srivatsa, Kenneth M Kaye, Utkan Demirci
Cancer cells have been increasingly grown in pharmaceutical research to understand tumorigenesis and develop new therapeutic drugs. Currently, cells are typically grown using two-dimensional (2-D) cell culture approaches, where the native tumor microenvironment is difficult to recapitulate. Thus, one of the main obstacles in oncology is the lack of proper infection models that recount main features present in tumors. In recent years, microtechnology-based platforms have been employed to generate three-dimensional (3-D) models that better mimic the native microenvironment in cell culture...
December 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27939840/niclosamide-inhibits-lytic-replication-of-epstein-barr-virus-by-disrupting-mtor-activation
#20
Lu Huang, Mengtian Yang, Yan Yuan, Xiaojuan Li, Ersheng Kuang
Infection with the oncogenic γ-herpesviruses Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) cause several severe malignancies in humans. Inhibition of the lytic replication of EBV and KSHV eliminates the reservoir of persistent infection and transmission, consequently preventing the occurrence of diseases from the sources of infection. Antiviral drugs are limited in controlling these viral infectious diseases. Here, we demonstrate that niclosamide, an old anthelmintic drug, inhibits mTOR activation during EBV lytic replication...
February 2017: Antiviral Research
keyword
keyword
11858
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"