Weiwei Zou, Kai Zong, Zhikang Zhang, Lingchao Shen, Xiaolei Wang, Xun Su, Xin Wang, Tao Yin, Chunmei Liang, Yajing Liu, Dan Liang, Chao Hu, Yunxia Cao, Dongmei Ji
PURPOSE: Although a variety of analytical methods have been developed to detect mitochondrial DNA (mtDNA) heteroplasmy, there are special requirements of mtDNA heteroplasmy quantification for women carrying mtDNA mutations receiving the preimplantation genetic diagnosis (PGD) and prenatal diagnosis (PD) in clinic. These special requirements include various sample types, large sample number, long-term follow-up, and the need for detection of single-cell from biopsied embryos. Therefore, developing an economical, accurate, high-sensitive, and single-cell analytical method for mtDNA heteroplasmy is necessary...
July 18, 2023: Journal of Assisted Reproduction and Genetics