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Stephanie Longet, Mimmi L E Lundahl, Ed Lavelle
Mucosal immune responses are in the first line of defence against most infections and protective mucosal immunity can be achieved by mucosal vaccination. However, mucosal tolerance and physicochemical features of the mucosal environment pose challenging obstacles to the development of mucosal vaccines. Vaccine formulations must be designed to enhance stability at the mucosae and incorporate features that induce innate immunity at mucosal inductive sites. To face these challenges, a number of novel delivery systems for targeting of mucosal vaccines to specific mucosal locations have been developed...
January 4, 2018: Bioconjugate Chemistry
Stephanie Longet, Vincenzo Aversa, Daire O'Donnell, Joshua Tobias, Monica Rosa, Jan Holmgren, Ivan S Coulter, Ed C Lavelle
Oral vaccines present an attractive alternative to injectable vaccines for enteric diseases due to ease of delivery and the induction of intestinal immunity at the site of infection. However, susceptibility to gastrointestinal proteolysis, limited transepithelial uptake and a lack of clinically acceptable adjuvants present significant challenges. A further challenge to mass vaccination in developing countries is the very expensive requirement to maintain the cold chain. We recently described the effectiveness of a Single Multiple Pill(®) (SmPill(®)) adjuvanted capsule approach to enhance the effectiveness of a candidate enterotoxigenic Escherichia coli (ETEC) oral vaccine...
October 9, 2017: International Journal of Pharmaceutics
Nima Tamaddoni, Graham Taylor, Trevor Hepburn, S Michael Kilbey, Stephen A Sarles
Biomimetic membranes assembled from block copolymers attract considerable interest because they exhibit greater stability and longetivity compared to lipid bilayers, and some enable the reconstitution of functional transmembrane biomolecules. Yet to-date, block copolymer membranes have not been achieved using the droplet interface bilayer (DIB) method, which uniquely allows assembling single- and multi-membrane networks between water droplets in oil. Herein, we investigate the formation of poly(ethylene oxide)-b-poly(dimethyl siloxane)-b-poly(ethylene oxide) triblock copolymer-stabilized interfaces (CSIs) between polymer-coated aqueous droplets in solutions comprising combinations of decane, hexadecane and AR20 silicone oil...
June 21, 2016: Soft Matter
Christopher J H Davitt, Edel A McNeela, Stephanie Longet, Joshua Tobias, Vincenzo Aversa, Craig P McEntee, Monica Rosa, Ivan S Coulter, Jan Holmgren, Ed C Lavelle
Diarrhoeal infections are a major cause of morbidity and mortality with enterotoxigenic Escherichia coli (ETEC) and cholera imposing a significant global burden. There is currently no licensed vaccine for ETEC. Development of new nonliving oral vaccines has proven difficult due to the physicochemical and immunological challenges associated with the oral route. This demands innovative delivery solutions to protect antigens, control their release and build in immune-stimulatory activity. We describe the Single Multiple Pill® (SmPill®) vaccine formulation which combines the benefits of enteric polymer coating to protect against low gastric pH, a dispersed phase to control release and aid the solubility of non-polar components and an optimized combination of adjuvant and antigen to promote mucosal immunity...
July 10, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
Josip Mikulic, Stéphanie Longet, Laurent Favre, Jalil Benyacoub, Blaise Corthesy
The importance of secretory IgA in controlling the microbiota is well known, yet how the antibody affects the perception of the commensals by the local immune system is still poorly defined. We have previously shown that the transport of secretory IgA in complex with bacteria across intestinal microfold cells results in an association with dendritic cells in Peyer's patches. However, the consequences of such an interaction on dendritic cell conditioning have not been elucidated. In this study, we analyzed the impact of the commensal Lactobacillus rhamnosus, alone or associated with secretory IgA, on the responsiveness of dendritic cells freshly recovered from mouse Peyer's patches, mesenteric lymph nodes, and spleen...
June 2017: Cellular & Molecular Immunology
Sugunakar Raju Godishala Swamy, Surapaneni Rateesh Kumar Nandan, Pavan G Kulkarni, Thokala Madhusudan Rao, Pavan Palakurthy
BACKGROUND: Xylene is a flammable liquid with characteristic petroleum or aromatic odours, it is miscible with most of the organic solvents and paraffin wax. Xylene clears tissues rapidly and renders transparency, facilitating clearing endpoint determination, this made it to be used as a clearing agent in routine histopathological techniques. Even though it is a good clearing agent, it causes damage to the tissues by its hardening effect particularly those fixed in non-protein coagulant fixatives...
November 2015: Journal of Clinical and Diagnostic Research: JCDR
Karine Meisser Redeuil, Karin Longet, Sylvie Bénet, Caroline Munari, Esther Campos-Giménez
This manuscript reports a validated analytical approach for the quantification of 21 water soluble vitamins and their main circulating forms in human plasma. Isotope dilution-based sample preparation consisted of protein precipitation using acidic methanol enriched with stable isotope labelled internal standards. Separation was achieved by reversed-phase liquid chromatography and detection performed by tandem mass spectrometry in positive electrospray ionization mode. Instrumental lower limits of detection and quantification reached <0...
November 27, 2015: Journal of Chromatography. A
Saima Jadoon, Sabiha Karim, Muhammad Hassham Hassan Bin Asad, Muhammad Rouf Akram, Abida Kalsoom Khan, Arif Malik, Chunye Chen, Ghulam Murtaza
The exposure to ultraviolet radiations (UVR) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS), leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts...
2015: Oxidative Medicine and Cellular Longevity
Stéphanie Longet, Cédric Vonarburg, Marius Lötscher, Sylvia Miescher, Adrian Zuercher, Blaise Corthésy
Intravenous administration of polyclonal and monoclonal antibodies has proven to be a clinically valid approach in the treatment, or at least relief, of many acute and chronic pathologies, such as infection, immunodeficiency, and a broad range of autoimmune conditions. Plasma-derived IgG or recombinant IgG are most frequently used for intravenous or subcutaneous administration, whereas a few IgM-based products are available as well. We have established recently that secretory-like IgA and IgM can be produced upon association of plasma-derived polymeric IgA and IgM with a recombinant secretory component...
August 1, 2014: Journal of Biological Chemistry
No abstract text is available yet for this article.
1953: Revue de Médecine Navale (métropole et Outre-mer); Travaux Scientifiques des Médecins et Pharmaciens-chimistes de la Marine
No abstract text is available yet for this article.
1953: Revue de Médecine Navale (métropole et Outre-mer); Travaux Scientifiques des Médecins et Pharmaciens-chimistes de la Marine
No abstract text is available yet for this article.
1953: Revue de Médecine Navale (métropole et Outre-mer); Travaux Scientifiques des Médecins et Pharmaciens-chimistes de la Marine
Lei Wang, Jia-Hua Zhang, Cai-E Cui, Ping Huang
Doubly doped YAG:Ce3+, Pb2+ phosphor was obtained by doping YAG:Ce3+ phosphor with PbO. Compared with the emission spectra of YAG:Ce3+ phosphor without PbO, the Ce3+ emission band of YAG:Ce3+, Pb2+ shifts to longet wave, which can enhance the red component of spectrum. Meanwhile, the intensity of Ce3+ emission is increased by 10% when the concentration of PbO is 5%, SEM image indicates that PbO may act as a flux. Temperature-dependent emission spectra of YAG: Ce3+, Pb2+ phosphor show a better thermal quenching characteristics than YAG:Ce3+ phosphor...
May 2013: Guang Pu Xue Yu Guang Pu Fen Xi, Guang Pu
Amandine Mathias, Stéphanie Longet, Blaise Corthésy
Shigella flexneri, by invading intestinal epithelial cells (IECs) and inducing inflammatory responses of the colonic mucosa, causes bacillary dysentery. Although M cells overlying Peyer's patches are commonly considered the primary site of entry of S. flexneri, indirect evidence suggests that bacteria can also use IECs as a portal of entry to the lamina propria. Passive delivery of secretory IgA (SIgA), the major immunoglobulin secreted at mucosal surfaces, has been shown to protect rabbits from experimental shigellosis, but no information exists as to its molecular role in maintaining luminal epithelial integrity...
August 2013: Infection and Immunity
Stéphanie Longet, Sarah Miled, Marius Lötscher, Sylvia M Miescher, Adrian W Zuercher, Blaise Corthésy
Immunotherapy with monoclonal and polyclonal immunoglobulin is successfully applied to improve many clinical conditions, including infection, autoimmune diseases, or immunodeficiency. Most immunoglobulin products, recombinant or plasma-derived, are based on IgG antibodies, whereas to date, the use of IgA for therapeutic application has remained anecdotal. In particular, purification or production of large quantities of secretory IgA (SIgA) for potential mucosal application has not been achieved. In this work, we sought to investigate whether polymeric IgA (pIgA) recovered from human plasma is able to associate with secretory component (SC) to generate SIgA-like molecules...
February 8, 2013: Journal of Biological Chemistry
Stéphanie Longet, John T Schiller, Martine Bobst, Patrice Jichlinski, Denise Nardelli-Haefliger
The available virus-like particle (VLP)-based prophylactic vaccines against specific human papillomavirus (HPV) types afford close to 100% protection against the type-associated lesions and disease. Based on papillomavirus animal models, it is likely that protection against genital lesions in humans is mediated by HPV type-restricted neutralizing antibodies that transudate or exudate at the sites of genital infection. However, a correlate of protection was not established in the clinical trials because few disease cases occurred, and true incident infection could not be reliably distinguished from the emergence or reactivation of prevalent infection...
December 2011: Journal of Virology
Dike B Ojji, Akinola O Babalola, Ayodele O Falase
Tetralogy of Fallot is the most common form of cyanotic congenital heart disease. Survival after the age of 12 years without corrective surgery is rare. We present the case of a 25 year-old man with uncorrected tetralogy of Fallot. Possible reasons for the longetivity in this patient are left ventricular hypertrophy and systemic to pulmonary shunting through internal mammary arteries.
2011: Clinical Medicine Insights. Case Reports
Mathieu Renouf, Karine Redeuil, Karin Longet, Cynthia Marmet, Fabiola Dionisi, Martin Kussmann, Gary Williamson, Kornél Nagy
BACKGROUND: Tea is an infusion of the leaves of the Camellia sinensis plant and is the most widely consumed beverage in the world after water. Green tea contains significant amounts of polyphenol catechins and represents a promising dietary component to maintain health and well-being. Epidemiological studies indicate that polyphenol intake may have potential health benefits, such as, reducing the incidence of coronary heart disease, diabetes and cancer. While bioavailability of green tea bioactives is fairly well understood, some gaps still remain to be filled, especially the identification and quantification of conjugated metabolites in plasma, such as, sulphated, glucuronidated or methylated compounds...
October 2011: European Journal of Nutrition
Kornél Nagy, Karine Redeuil, Gary Williamson, Serge Rezzi, Fabiola Dionisi, Karin Longet, Frédéric Destaillats, Mathieu Renouf
There is a substantial amount of published literature on the bioavailability of various coffee components including the most abundant metabolites, caffeic and ferulic acids. Surprisingly, to date, the appearance of dimethoxycinnamic acid derivatives in humans has not been reported despite the fact that methylated form of catechol-type polyphenols could help maintain, modify or even improve their biological activities. This study reports an LC-MS method for the detection of dimethoxycinnamic acid in human plasma after treatment with an esterase...
January 21, 2011: Journal of Chromatography. A
Ossama Abbas, Joanna E Richards, Ron Yaar, Meera Mahalingam
The inherent longetivity of stem cells causes them to be susceptible to multiple genetic hits. Thus, it is not surprising that stem cells are implicated in the etiopathogenesis of select cutaneous neoplasms. However, most studies to date are restricted to the use of a single marker (p63, cytokeratin-15 or cytokeratin-19) and do not appear to compare distribution of stem cell markers in a spectrum of cutaneous in situ versus invasive epithelial malignancies. In this study, we evaluate expression of cytokeratin-15, cytokeratin-19, and p63 in a series of primary cutaneous epithelial lesions that include actinic keratosis (n=29), squamous cell carcinoma in situ (n=30), bowenoid papulosis (n=15) and squamous cell carcinoma, well differentiated (n=29) in order to evaluate the role of stem cell marker expression in the grading and development of in situ and invasive malignancies...
January 2011: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
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