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https://www.readbyqxmd.com/read/27916261/cis-bifenthrin-causes-immunotoxicity-in-murine-macrophages
#1
Xia Wang, Xingli Gao, Bingnan He, Yuanxiang Jin, Zhengwei Fu
Synthetic pyrethroids (SPs) are commonly used insecticides that have been detected in mammals, including humans, indicating a potential threat to human health. Bifenthrin (BF), as well as other pyrethroids, has been shown to possess neurotoxic, reproductive, hepatotoxic and nephrotoxic potential in mammals. However, studies regarding the immunotoxicity of BF and its mechanism are limited. In this study, we aim to exam the immunotoxicity of cis-BF on the murine macrophage cell line, RAW 264.7. MTT assay results demonstrated that cis-BF exposure induced apoptosis in RAW 264...
December 1, 2016: Chemosphere
https://www.readbyqxmd.com/read/27871895/protective-effect-of-lycopene-on-fluoride-induced-ameloblasts-apoptosis-and-dental-fluorosis-through-oxidative-stress-mediated-caspase-pathways
#2
Weishan Li, Binghua Jiang, Xianglin Cao, Yongjiang Xie, Ting Huang
Fluoride is an environmental toxicant and induces dental fluorosis and oxidative stress. Lycopene (LYC) is an effective antioxidant that is reported to attenuate fluoride toxicity. To determine the effects of LYC on sodium fluoride (NaF) -induced teeth and ameloblasts toxicity, rats were treated with NaF (10 mg/kg) and/or LYC (10 mg/kg) by orally administration for 5 weeks; ameloblasts were treated with NaF (5 mM) and/or LYC (2 μM) for 6 h. We found that the concentrations of fluoride, malondialdehyde (MDA) and reactive oxygen species (ROS), gene expressions and activities of Caspase-9 and Caspase-3, and the gene expressions of Bax were significantly decreased, while the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX), the gene expression of Bcl-2 were significantly increased in the LYC + NaF-treated rats group; concentrations of MDA and ROS, gene expressions and activities of Caspase-9 and Caspase-3, and the gene expression of Bax, and ameloblasts apoptosis rate were significantly decreased, while the activities of SOD and GPX, the gene expression of Bcl-2 were significantly increased in the LYC + NaF-treated ameloblasts group...
November 18, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27855397/inhibition-of-cartilage-acidic-protein-1-reduces-ultraviolet-b-irradiation-induced-apoptosis-through-p38-mitogen-activated-protein-kinase-and-jun-amino-terminal-kinase-pathways
#3
Yinghong Ji, Xianfang Rong, Dan Li, Lei Cai, Jun Rao, Yi Lu
BACKGROUND/AIMS: Ultraviolet B (UVB) irradiation can easily induce apoptosis in human lens epithelial cells (HLECs) and further lead to various eye diseases including cataract. Here for the first time, we investigated the role of cartilage acidic protein 1 (CRTAC1) gene in UVB irradiation induced-apoptosis in HLECs. METHODS: Three groups of HLECs were employed including model group, empty vector group, and CRTAC1 interference group. RESULTS: After UVB irradiation, the percentage of primary apoptotic cells was obviously fewer in CRTAC1 interference group...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27855372/small-molecule-th-39-potentially-targets-hec1-nek2-interaction-and-exhibits-antitumor-efficacy-in-k562-cells-via-g0-g1-cell-cycle-arrest-and-apoptosis-induction
#4
Yongxia Zhu, Wei Wei, Tinghong Ye, Zhihao Liu, Li Liu, Yong Luo, Lidan Zhang, Chao Gao, Ningyu Wang, Luoting Yu
BACKGROUND: Cancer is still a major public health issue worldwide, and new therapeutics with anti-tumor activity are still urgently needed. METHODS: The anti-tumor activity of TH-39, which shows potent anti-proliferative activity against K562 cells with an IC50 of 0.78 µM, was investigated using immunoblot, co-immunoprecipitation, the MTT assay, and flow cytometry. RESULTS: Mechanistically, TH-39 may disrupt the interaction between Hec1 and Nek2 in K562 cells...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27827932/bauhinia-championii-flavone-attenuates-hypoxia-reoxygenation-induced-apoptosis-in-h9c2-cardiomyocytes-by-improving-mitochondrial-dysfunction
#5
Ping Liao, Guibo Sun, Chan Zhang, Min Wang, Yao Sun, Yuehan Zhou, Xiaobo Sun, Jie Jian
This study aimed to determine the effects of Bauhinia championii flavone (BCF) on hypoxia-reoxygenation (H/R) induced apoptosis in H9c2 cardiomyocytes and to explore potential mechanisms. The H/R model in H9c2 cardiomyocytes was established by 6 h of hypoxia and 12 h of reoxygenation. Cell viability was detected by CCK-8 assay. Apoptotic rate was measured by Annexin V/PI staining. Levels of mitochondria-associated ROS, mitochondrial transmembrane potential (∆Ψm) and mitochondrial permeability transition pores (MPTP) opening were assessed by fluorescent probes...
November 4, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27816647/synthesis-characterization-and-anticancer-effect-of-the-ruthenium-ii-polypyridyl-complexes-on-hepg2-cells
#6
Dan Wan, Shang-Hai Lai, Hui-Hui Yang, Bing Tang, Cheng Zhang, Hui Yin, Chuan-Chuan Zeng, Yun-Jun Liu
As one of the major cell regulated center, mitochondria are closely associated with cell proliferation, apoptosis of tumor cell. In this work, four new ruthenium (II) polypyridyl complexes [Ru(bpy)2(FTTP)](ClO4)2 (1) (FTTP=11-(3-fluoro-naphthalen-2-yloxy)-4,5,9,14-tetraaza-benzo[b]triphenylene, bpy=2,2'-bipyridine), [Ru(phen)2(FTTP)](ClO4)2 (2) (phen=1,10-phenanthroline), [Ru(bpy)2(PTTP)](ClO4)2 (3) (PTTP=2-phenoxy-1,4,8,9-tetraazatriphenylene) and [Ru(phen)2(PTTP)](ClO4)2 (4) were synthesized and characterized by elemental analysis, ESI-MS, (1)H NMR and (13)C NMR...
October 31, 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27796592/the-induction-of-apoptosis-in-sgc-7901-cells-through-the-ros-mediated-mitochondrial-dysfunction-pathway-by-a-ir-iii-complex
#7
Cheng Zhang, Shang-Hai Lai, Chuan-Chuan Zeng, Bing Tang, Dan Wan, De-Gang Xing, Yun-Jun Liu
A new ligand BTCP and its iridium(III) complex [Ir(ppy)2(BTCP)]PF6 (Ir-1) were synthesized and characterized by elemental analysis, ESI-MS, IR, (1)H NMR and (13)C NMR. The cytotoxic activity in vitro of the ligand and its complex against SGC-7901, HeLa, HOS, PC-12, BEL-7402, MG-63, SiHa, A549, HepG2 and normal cell LO2 were evaluated by MTT method [MTT = (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)]. The apoptosis was assayed with AO/EB and Hoechst 33258 staining methods. The reactive oxygen species (ROS), mitochondrial membrane potential, autophagy and cell invasion were studied under fluorescent microscope...
October 28, 2016: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/27787886/neuroglobin-protects-astroglial-cells-from-hydrogen-peroxide-induced-oxidative-stress-and-apoptotic-cell-death
#8
Fatma Amri, Ikram Ghouili, Mohamed Amri, Alice Carrier, Olfa Masmoudi-Kouki
Oxidative stress, resulting from accumulation of reactive oxygen species (ROS), plays a critical role in astroglial cell death occurring in diverse neuropathological conditions. Numerous studies indicate that neuroglobin (Ngb) promotes neuron survival, but nothing is known regarding the action of Ngb in astroglial cell survival. Thus, the purpose of the present study was to investigate the potential glioprotective effect of Ngb on hydrogen peroxide (H2 O2 )-induced oxidative stress and apoptosis in cultured mouse astrocytes...
October 27, 2016: Journal of Neurochemistry
https://www.readbyqxmd.com/read/27764754/tetrandrine-protects-against-oxygen-glucose-serum-deprivation-reoxygenation-induced-injury-via-pi3k-akt-nf-%C3%AE%C2%BAb-signaling-pathway-in-rat-spinal-cord-astrocytes
#9
Gang Bao, Chuankun Li, Lei Qi, Ning Wang, Baixiang He
Tetrandrine (TET) is a bis-benzylisoquinoline alkaloid, which is isolated from a Chinese medicinal herb with antioxidant and anti-inflammatory activities. In this study, we investigated the effects of TET on oxygen-glucose-serum deprivation/reoxygenation (OGSD/R)-induced injury in rat spinal cord astrocytes, which mimics hypoxic/ischemic conditions in vivo. MTT and LDH assays indicated that cell viability was distinctly reduced and LDH leakage was elevated after OGSD/R exposure, which were dose-dependently reversed by pretreatment with TET (0...
October 17, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27728894/madecassic-acid-protects-against-hypoxia-induced-oxidative-stress-in-retinal-microvascular-endothelial-cells-via-ros-mediated-endoplasmic-reticulum-stress
#10
Boyu Yang, Yue Xu, Yaguang Hu, Yiwen Luo, Xi Lu, Ching Kit Tsui, Lin Lu, Xiaoling Liang
Madecassic acid (MA) is an abundant triterpenoid in Centella asiatica (L.) Urban. (Apiaceae) that has been used as a wound-healing, anti-inflammatory and anti-cancer agent. Up to now, the effects of MA against oxidative stress remain unclear. In this study, we investigated the effect of MA and its mechanisms on hypoxia-induced human Retinal Microvascular Endothelial Cells (hRMECs). hRMECs were pre-treated with different concentrations of MA (0-50μM) for 30min before being incubated under hypoxia condition (37°C, 5% CO2 and 95% N2)...
October 8, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27718470/antagonizing-stat3-activation-with-benzo-b-thiophene-1-1-dioxide-based-small-molecules
#11
Wenda Zhang, Ting Ma, Shanshan Li, Yanwei Yang, Jianpeng Guo, Wenying Yu, Lingyi Kong
STAT3 is an attractive therapeutic target for cancer therapy. However, due to low potency or poor druggability, none of its inhibitors are clinically available. Herein, a series of aminobenzo[b]thiophene 1, 1-dioxides with good drug-likeness properties were designed, synthesized and evaluated as STAT3 inhibitors. Most of them exhibited higher antitumor activity than the small-molecule STAT3 inhibitor, Stattic. Compound 15 was the most potent and had an IC50 range in 0.33-0.75 μM in various cancer cell lines...
September 22, 2016: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27706063/a-potential-mechanism-for-the-anti-apoptotic-property-of-koumine-involving-mitochondrial-pathway-in-lps-mediated-raw-264-7-macrophages
#12
Zhi-Hang Yuan, Zeng-Enni Liang, Jing Wu, Jin-E Yi, Xiao-Jun Chen, Zhi-Liang Sun
Koumine is a kind of alkaloid extracted from Gelsemium elegans (G. elegans). Benth, which has shown promise as an anti-tumor, anxiolytic, and analgesic agent. In our present study, the effect of koumine on lipopolysaccharide (LPS)-mediated RAW 264.7 cell apoptosis was evaluated. MTT assays showed that koumine obviously increased cell viability in LPS-mediated RAW 264.7 macrophages. Preincubation with koumine ameliorated LPS-medicated apoptosis by decreasing reactive oxygen species (ROS) production, which resulted in a significant decrease in the levels of nitric oxide (NO) and inducible nitric oxide synthase (iNOS)...
September 30, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27687210/lack-of-mitochondrial-dna-impairs-chemical-hypoxia-induced-autophagy-in-liver-tumor-cells-through-ros-ampk-ulk1-signaling-dysregulation-independently-of-hif-1%C3%AE
#13
Jose J G Marin, Elisa Lozano, Maria J Perez
Alterations in mitochondrial DNA (mtDNA) and autophagy activation are common events in tumors. Here we have investigated the effect of mitochondrial genome depletion on chemical hypoxia-induced autophagy in liver tumor cells. Human SK-Hep-1 wild-type and mtDNA-depleted (Rho) cells were exposed to the hypoxia mimetic agents CoCl2 and deferoxamine (DFO). Up-regulation of HIF-1α, but not HIF-2α was observed. The expression of several HIF-1α target genes was also found. In human SK-Hep-1 and mouse Hepa 1-6 liver tumor cells, but not in the counterpart Rho derived lines, chemical hypoxia increased the abundance of autophagosomes and autolysosomes...
September 26, 2016: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/27627918/inflammatory-and-apoptotic-regulatory-activity-of-tanshinone-iia-in-helicobacter-pylori-infected-cells
#14
Guan-Yu Chen, Yu-Chieh Shu, Duen-Yau Chuang, Yuan-Chuen Wang
Helicobacter pylori infections induce host cell inflammation and apoptosis, however, they are conflicting. Tanshinone IIA is an active compound of Salvia miltiorrhiza Bge. In this study, we investigated the regulatory effects of tanshinone IIA on H. pylori-induced inflammation and apoptosis in vitro. Tanshinone IIA treatments (13.6-54.4[Formula: see text][Formula: see text]M) significantly decreased nuclear factor kappa B (NF-kB) and mitogen-activated protein kinase (MAPK) [p-38 and C-terminal Jun-kinase 1/2 (JNK1/2)] protein expressions and inflammatory substance [cyclooxygenase-2 (COX-2), 5-lipooxygenase (5-LOX), intercellular adhesion molecule-1 (ICAM-1), reactive oxygen species (ROS), nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1[Formula: see text] (IL-1[Formula: see text], IL-6, and IL-8] production in the H...
2016: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/27618007/bl-038-a-benzofuran-derivative-induces-cell-apoptosis-in-human-chondrosarcoma-cells-through-reactive-oxygen-species-mitochondrial-dysfunction-and-the-caspases-dependent-pathway
#15
Ju-Fang Liu, Chien-Yu Chen, Hsien-Te Chen, Chih-Shiang Chang, Chih-Hsin Tang
Chondrosarcoma is a highly malignant cartilage-forming bone tumor that has the capacity to invade locally and cause distant metastasis. Moreover, chondrosarcoma is intrinsically resistant to conventional chemotherapy or radiotherapy. The novel benzofuran derivative, BL-038 (2-amino-3-(2,6-dichlorophenyl)-6-(4-methoxyphenyl)benzofuran-4-yl acetate), has been evaluated for its anticancer effects in human chondrosarcoma cells. BL-038 caused cell apoptosis in two human chondrosarcoma cell lines, JJ012 and SW1353, but not in primary chondrocytes...
2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27514534/epa-or-dha-enhanced-oxidative-stress-and-aging-protein-expression-in-brain-of-d-galactose-treated-mice
#16
Yuan-Man Hsu, Mei-Chin Yin
BACKGROUND: Effects of eicosapentaenoic acid (EPA, 20:5) and docosahexaenoic acid (DHA, 22:6) upon fatty acid composition, oxidative and inflammatory factors and aging proteins in brain of d-galactose (DG) treated aging mice were examined. METHODS: Each fatty acid at 7 mg/kg BW/week was supplied for 8 weeks. Brain aging was induced by DG treatment (100 mg/kg body weight) via daily subcutaneous injection for 8 weeks. RESULTS: DG, EPA and DHA treatments changed brain fatty acid composition...
June 2016: BioMedicine
https://www.readbyqxmd.com/read/27470359/-6-shogaol-a-dietary-phenolic-compound-induces-oxidative-stress-mediated-mitochondrial-dependant-apoptosis-through-activation-of-proapoptotic-factors-in-hep-2-cells
#17
Govindhan Annamalai, Suresh Kathiresan, Nagappan Kannappan
Ginger (Zingiber officinale) is a well-known herb used in ethnomedicine. [6]-shogaol, a phenolic nature is a major constituent of ginger. In this study, we investigated the anticancer activity of [6]-shogaol in Laryngeal cancer (Hep-2) cells. We demonstrated the effects of [6]-shogaol on the cell growth and apoptosis in Hep-2 cells were analyzed by the generation of reactive oxygen species (ROS), the level of mitochondrial membrane potential (ΔYm), DNA damage and apoptotic morphological changes were analyzed by AO/EtBr, AO and Hoechst staining...
August 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27451028/6-gingerol-induces-autophagy-to-protect-huvecs-survival-from-apoptosis
#18
Shaopeng Wang, Xiance Sun, Liping Jiang, Xiaofang Liu, Min Chen, Xiaofeng Yao, Qinghua Sun, Guang Yang
6-Gingerol, the major pharmacologically-active component of ginger, has the potential to prevent heart disease. However, the mechanisms are not well understood. In this study, the protective effect of 6-gingerol against hydrogen peroxide-induced apoptosis in human umbilical vein endothelial cells (HUVECs) was investigated. Apoptosis was detected by Hoechst 33342 and Flow cytometry analysis. To further elucidate the crosstalk between apoptosis and autophagy, we tested the expression of autophagy related proteins, LC3B, Bcl-2, Beclin1, AKT, p-AKT, mechanistic target of rapamycin (mTOR), and p-mTOR...
August 25, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27377460/inhibitory-effect-on-ht-29-colon-cancer-cells-of-a-water-soluble-polysaccharide-obtained-from-highland-barley
#19
Dai Cheng, Xinyu Zhang, Meng Meng, Lirong Han, Caijiao Li, Lihua Hou, Wentao Qi, Chunling Wang
A water-soluble polysaccharide (BP-1) was obtained from highland barley (Hordeum vulgare L.) by hot water extraction and purification of sepharose column chromatography. BP-1 had an average molecular weight of about 6.7×10(4)Da and was composed of glucose (Glc), xylose (Xyl), arabinose (Ara) and rhamnose (Rha) with a relative molar ratio of 8.82:1.92:1.50:1.00. It was found that BP-1 inhibited proliferation of human colon cancer cells (HT-29) in a time- and dose-dependent manner with half maximal inhibitory concentration at 48h of 48...
July 1, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27367721/mangiferin-and-cancer-mechanisms-of-action
#20
REVIEW
Fuchsia Gold-Smith, Alyssa Fernandez, Karen Bishop
Mangiferin, a bioactive compound derived primarily from Anacardiaceae and Gentianaceae families and found in mangoes and honeybush tea, has been extensively studied for its therapeutic properties. Mangiferin has shown promising chemotherapeutic and chemopreventative potential. This review focuses on the effect of mangiferin on: (1) inflammation, with respect to NFκB, PPARү and the immune system; (2) cell cycle, the MAPK pathway G₂/M checkpoint; (3) proliferation and metastasis, and implications on β-catenin, MMPs, EMT, angiogenesis and tumour volume; (4) apoptosis, with a focus on Bax/Bcl ratios, intrinsic/extrinsic apoptotic pathways and telomerase activity; (5) oxidative stress, through Nrf2/ARE signalling, ROS elimination and catalase activity; and (6) efficacy of chemotherapeutic agents, such as oxaliplatin, etoposide and doxorubicin...
2016: Nutrients
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