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ros, bcl-6,bcl-2,

Yashika Awasthi, Arun Ratn, Rajesh Prasad, Manoj Kumar, Sunil P Trivedi
Present study was designed to assess the hexavalent chromium (Cr6+ ) mediated oxidative stress that induces DNA damage and apoptosis in adult fish, Channa punctatus (35 ± 3.0 g; 14.5 ± 1.0 cm; Actinopterygii). Fishes were maintained in three groups for 15, 30 and 45 d of exposure periods. They were treated with 5% (Group T1) and 10% (Group T2) of 96 h-LC50 of chromium trioxide (Cr6+ ). Controls were run for the similar duration. A significant (p < 0.05) increment in the activities of antioxidant enzymes, SOD and CAT in liver tissues of the exposed fish evinces the persistence of oxidative stress...
May 5, 2018: Aquatic Toxicology
Yang He, Lingyun Zhou, Zhiqiang Fan, Shikun Liu, Weijin Fang
Pharmacological inhibition of reactive oxygen species (ROS) is a potential strategy to prevent diabetes-induced cardiac dysfunction. This study was designed to investigate precise effects of antioxidant N‑acetylcysteine (NAC) in alleviating diabetic cardiomyopathy (DCM). Echocardiography and histologic studies were performed 12 weeks after streptozocin injection. Protein levels involved in endoplasmic reticulum stress (ERS) and apoptosis were analyzed by western blotting in diabetic hearts or high-glucose (HG, 30 mM)- and palmitic acid (PA, 300 μM)-cultured neonatal rat cardiomyocytes (NRCMs)...
May 11, 2018: Cell Death & Disease
Rashmi Shukla, Somanshu Banerjee, Yamini B Tripathi
BACKGROUND: Oxidative stress and renal apoptosis play a significant role in the progression of diabetic nephropathy. The tubers of Pueraria tuberosa (Roxb. ex Willd.) DC. has been traditionally used as anti-ageing and health promotive tonic. The purpose of this study was to investigate its nephroprotective effect and mechanism via antioxidant and antiapoptotic potential in Streptozotocin-induced diabetic nephropathy (DN) in rats. METHODS: The chemical composition of aqueous extract of Pueraria tuberosa (PTY-2r) was analyzed by gas chromatography-mass spectrometry (GC-MS)...
May 11, 2018: BMC Complementary and Alternative Medicine
Hyunjin Jeong, Yumi Shin, Dong-Kyoo Kim
In the present study, the anticancer activity of 1‑[(3S,4R)‑2,2‑dimethyl‑3‑oxo‑4‑(2‑piperidonyl)chroman‑6‑yl]‑3‑phenylurea (S32) was investigated by testing its effect in vitro on the growth of HeLa cells. First, we showed that the IC50 value of S32 was ~70 µM by using WST‑8 assay, and that it significantly inhibited the proliferation and viability of HeLa cells in a dose‑dependent manner after 48 h. Morphological changes in apoptotic cells included cellular shrinkage and nuclear condensation...
April 27, 2018: Oncology Reports
X Chen, R Gu, L Zhang, J Li, Y Jiang, G Zhou, F Gao
The oxidative study has always been particularly topical in poultry science. However, little information about the occurrence of cellular apoptosis and autophagy through the reactive oxygen species (ROS) generation in nuclear factor-κB (NF-κB) signal pathway was reported in the liver of broilers exposed to hydrogen peroxide (H2O2). So we investigated the change of growth performance of broilers exposed to H2O2 and further explored the occurrence of apoptosis and autophagy, as well as the expression of NF-κB in these signaling pathways in the liver...
May 3, 2018: Animal: An International Journal of Animal Bioscience
Yu Liu, Pei-Hao Wen, Xin-Xue Zhang, Yang Dai, Qiang He
Acute liver injury is characterized by fibrosis, inflammation and apoptosis, leading to liver failure, cirrhosis or cancer and affecting the clinical outcome in the long term. However, no effective therapeutic strategy is currently available. Breviscapine, a mixture of flavonoid glycosides, has been reported to have multiple biological functions. The present study aimed to investigate the effects of breviscapine on acute liver injury induced by CCl4 in mice. C57BL/6 mice were subjected to intraperitoneal injection with CCl4 for 8 weeks with or without breviscapine (15 or 30 mg/kg)...
May 2, 2018: International Journal of Molecular Medicine
Li Lu, Qianyi Lu, Wei Chen, Jingwen Li, Chunxia Li, Zhi Zheng
Purpose: This study aimed to evaluate the mechanisms underlying the effects of 1,25-dihydroxyvitamin D (vitamin D3 ) on diabetes-induced retinal vascular damage and retinal vascular endothelial cell apoptosis. Methods: Diabetic and control rats were randomly assigned to receive vitamin D3 or vehicle for 6 months. Additionally, human retinal microvascular endothelial cells (HRMECs) were incubated in normal or high-glucose medium with or without vitamin D3 . Morphological changes in retinal tissues and retinal vascular permeability were examined, and cellular apoptosis was detected by fluorescence staining...
2018: Journal of Diabetes Research
Y Chandrasekhar, G Phani Kumar, E M Ramya, K R Anilakumar
Gallic acid is one of the most important polyphenolic compounds, which is considered an excellent free radical scavenger. 6-Hydroxydopamine (6-OHDA) is a neurotoxin, which has been implicated in mainly Parkinson's disease (PD). In this study, we investigated the molecular mechanism of the neuroprotective effects of gallic acid on 6-OHDA induced apoptosis in human dopaminergic cells, SH-SY5Y. Our results showed that 6-OHDA induced cytotoxicity in SH-SY5Y cells was suppressed by pre-treatment with gallic acid...
April 18, 2018: Neurochemical Research
Jiangquan Fu, Qing Lan, Difen Wang, Ying Wang, Ying Liu
OBJECTIVE: To observe the effect of hydrogen-rich water on the chondriosome damage and cytokines change in brain tissue of rats with traumatic brain injury (TBI). METHODS: Fifty-four health male Sprague-Dawley (SD) rats were divided into three groups by random number table: sham group, trauma group (TBI group), and trauma+hydrogen-rich water group (TBI+HW group), the rats in each group were subdivided into 1, 3 and 7 days subgroups according to the time points after trauma, with 6 rats in each subgroup...
April 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Wen-Yao Zhang, Qian-Yan Yi, Yang-Jie Wang, Fan Du, Miao He, Bing Tang, Dan Wan, Yun-Jun Liu, Hong-Liang Huang
Three new iridium (III) complexes [Ir (ppy)2 (ipbc)](PF6 ) (1), [Ir (bzq)2 (ipbc)](PF6 ) (2) and [Ir (piq)2 (ipbc)](PF6 ) (3) were designed and synthesized. All the complexes were tested for anticancer activity using 3-(4,5-dimethylthiazole)-2,5-diphenyltetraazolium bromide (MTT) method. The complexes show no cytotoxic activity toward cancer BEL-7402, SGC-7901, Eca-109, A549, HeLa and HepG2 cells. However, upon irradiation with white light, the complexes display high cytotoxicity against BEL-7402 cells with an IC50 value of 5...
April 7, 2018: European Journal of Medicinal Chemistry
Dong Zhen, Ling Su, Yue Miao, Fei Zhao, Guihong Ren, Shad Mahfuz, Hui Song
In this study, a homogeneous polysaccharide (designated as GAP-3S) with an average molecular weight of 6.82×105 Da was purified from Ganoderma applanatum. GAP-3S was composed of glucose, galactose, fucose and xylose in the molar ratio of 7.1:2.6:1.1:1. It could significantly inhibit the proliferation of MCF-7 cells in a dose- and time-dependent manner. Flow cytometry analysis indicated that GAP-3S could also induce cell apoptosis. Treatment of MCF-7 cells with GAP-3S lead to the collapse of mitochondrial membrane potential and the accumulation of ROS, up-regulated expression of Bax, cleavage of PARP, p-p38 and p-JNK, and the down-regulated expression of Bcl-2, PARP and p-ERK...
April 10, 2018: International Journal of Biological Macromolecules
Madhubanti Mullick, Dwaipayan Sen
Hypoxia-reperfusion (H/R) emblems a plethora of pathological conditions which is potent in contributing to the adversities encountered by human mesenchymal stem cells (hMSCs) in post-transplant microenvironment, resulting in transplant failure. D-Alanine 2, Leucine 5 Enkephaline (DADLE)-mediated delta opioid receptor (DOR) activation is well-known for its recuperative properties in different cell types like neuronal and cardiomyocytes. In the current study its effectiveness in assuaging hMSC mortality under H/R-like insult has been delineated...
April 12, 2018: Stem Cell Reviews
Xiuge Gao, Yani Zheng, Lin Peng, Xiangchun Ruan, Hui Ji, Yawei Qiu, Xiaoxiao Liu, Pei Teng, Dawei Guo, Shanxiang Jiang
Maduramicin is one of the most extensively used anticoccidial drugs for the treatment of Eimeria spp. infections. However, overdosage, misuse and drug interactions have resulted in the development of ionophore toxic syndrome. Heart and skeletal muscles have been identified as the main target organs of toxicity. In the present study, primary chicken myocardial cells were isolated to investigate the toxicity and underlying mechanisms of maduramicin. Our results showed that maduramicin causes morphological changes and a decrease in the viability of chicken myocardial cells...
March 23, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Lingling Si, Xinyan Yan, Wenjin Hao, Xiaoyi Ma, Huanhuan Ren, Boxue Ren, Defang Li, Zhengping Dong, Qiusheng Zheng
The aim of the present study was to determine the effects of Licochalcone D (LD) on the apoptosis and migration and invasion in human melanoma A375 cells. Cell proliferation was determined by sulforhodamine B assay. Apoptosis was assessed by Hoechst 33258 and Annexin V‑FITC/PI staining and JC‑1 assay. Total intracellular reactive oxygen species (ROS) was examined by DCFH‑DA. Wound healing and Transwell assays were used to detect migration and invasion of the cells. The activities of matrix metalloproteinase (MMP‑2 and MMP‑9) were assessed via gelatin zymography...
May 2018: Oncology Reports
Martin Busch, Susanne Wasmuth, Georg Spital, Albrecht Lommatzsch, Daniel Pauleikhoff
BACKGROUND: Retinal pigment epithelial (RPE) cells undergo functional changes upon complement stimulation, which play a role in the pathogenesis of age-related macular degeneration (AMD). These effects are in part enhanced by pretreating ARPE-19 cells with UV-irradiated photoreceptor outer segments (UV-POS) in vitro. The aim of this study was to investigate the effects of human complement serum (HCS) treatment on p44/42 mitogen-activated protein kinase (extracellular signal-regulated kinase 1/2 [ERK1/2]) activation in ARPE-19 cells pretreated with UV-POS...
February 27, 2018: Ophthalmologica. Journal International D'ophtalmologie
Ling-Zhi Ding, Xiao Teng, Zhao-Bo Zhang, Chang-Jun Zheng, Shi-Hong Chen
Mangiferin is a xanthone glucoside, which possesses antioxidant, antiviral, antitumor and anti-inflammatory functions, and is associated with gene regulation. However, it remains unknown whether mangiferin protects osteoblasts, such as the MC3T3-E1 cell line, against glucocorticoid-induced damage. In the present study, MC3T3-E1 cells were treated with dexamethasone (Dex), which is a well-known synthetic glucocorticoid, in order to establish a glucocorticoid-induced cell injury model. After Dex and/or mangiferin treatment, cell viability, apoptosis and reactive oxygen species (ROS) production was measured by Cell Counting kit-8 (CCK-8) and flow cytometry, respectively, and the concentration of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and macrophage colony-stimulating factor (M-CSF) was measured by ELISA...
May 2018: International Journal of Molecular Medicine
Ling-Yu Li, Jia-Di Peng, Wenjuan Zhou, Hui Qiao, Xin Deng, Zhou-Hua Li, Ji-Deng Li, Yun-Dong Fu, Song Li, Kai Sun, Hong-Min Liu, Wen Zhao
Hydrazone and their derivatives are a series of highly active molecules, which are widely used as lead compounds for the research and development of new anti-cancer drugs. In this study, 20 compounds were synthesized, based on this scaffold and their in vitro cytotoxicity against 6 cancer cell lines, including EC9706, SMMC-7721, MCF7, PC3, MGC-803 and EC109 was tested. Among them, compound 6p, showed strong anti-proliferative activities on esophageal carcinoma cells: EC9706 and EC109 with IC50 values of 1...
March 25, 2018: European Journal of Medicinal Chemistry
Ji Hoon Song, Dahae Lee, Seoung Rak Lee, Jae Sik Yu, Tae Su Jang, Joo-Won Nam, Ki Hyun Kim, Ki Sung Kang
A phytochemical investigation of the MeOH extracts from mulberries (the fruit of Morus alba L.) led to the identification of six heterocyclic compounds (1‑6). These compounds were screened to detect whether they protected pancreatic INS‑1 cells from streptozotocin (STZ)‑induced cytotoxicity. Compound 3 was the most effective at preventing STZ‑induced cytotoxicity and the production of reactive oxygen species (ROS) in INS‑1 cells. In addition, compound 3 effectively prevented apoptosis induced by STZ in INS‑1 cells...
April 2018: Molecular Medicine Reports
S-W Wang, L-X Deng, H-Y Chen, Z-Q Su, S-L Ye, W-Y Xu
OBJECTIVE: The apoptosis of vascular endothelial cells (VEC) is related to ischemic stroke. Phosphatidylinositol-3 kinase (PI3K)/protein kinase B (AKT/PKB) signaling pathway can upregulate Bcl-2 expression, reduce reactive oxygen species (ROS) production, and induce apoptosis. The level of miR-124 was significantly increased after cerebral ischemia. This study aimed to investigate the role of miR-124 in regulating PI3K expression, brain VEC apoptosis, and ROS production. MATERIALS AND METHODS: The expressions of miR-124, PI3K, p-AKT, and Bcl-2 in brain VEC of rats from the sham group and middle cerebral artery occlusion (MCAO) group were tested...
January 2018: European Review for Medical and Pharmacological Sciences
Qian Sun, Na-Na Lu, Lei Feng
Apigetrin (APG), as a flavonoid, has many cellular bioactivities, including regulation of oxidative stress, and induction of apoptosis. However, the means by which APG suppresses human gastric cancer are still little to be understood. In the present study, the anti-cancer effects of APG on human gastric cancer cells were investigated. The results indicated that APG could suppress the proliferation and induce apoptosis in gastric cancer cells. Its role in apoptosis induction was through reducing Bcl-2, and enhancing Bax, Caspase-9/-3 and poly ADP-ribose polymerase (PARP) cleavage...
March 25, 2018: Biochemical and Biophysical Research Communications
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