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https://www.readbyqxmd.com/read/28444183/calcium-dysregulation-and-cdk5-atm-pathway-involved-in-a-mouse-model-of-fragile-x-associated-tremor-ataxia-syndrome
#1
Gaëlle Robin, José R López, Glenda M Espinal, Susan Hulsizer, Paul J Hagerman, Isaac N Pessah
Fragile X-associated tremor/ataxia syndrome (FXTAS) is a neurological disorder that affects premutation carriers with 55-200 CGG-expansion repeats (preCGG) in FMR1, presenting with early alterations in neuronal network formation and function that precede neurodegeneration. Whether intranuclear inclusions containing DNA damage response (DDR) proteins, are causally linked to abnormal synaptic function, neuronal growth and survival are unknown. In a mouse that harbors a premutation CGG expansion (preCGG), cortical and hippocampal FMRP expression is moderately reduced from birth through adulthood, with greater FMRP reductions in the soma than in the neurite, despite several-fold elevation of Fmr1 mRNA levels...
April 21, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28440462/imperatorin-efficiently-blocks-tnf-%C3%AE-mediated-activation-of-ros-pi3k-akt-nf-%C3%AE%C2%BAb-pathway
#2
Ke Si Wang, Ying Lv, Zhe Wang, Juan Ma, Chunliu Mi, Xuezheng Li, Guang Hua Xu, Lian Xun Piao, Sheng Zhe Zheng, Xuejun Jin
Inflammation contributes to development and progression in a variety of cancers, including cervical cancer, which is the second leading cause of cancer deaths in women worldwide. In this study, we examined the anti-inflammatory effects of imperatorin, a psoralen-type furanocoumarin from the fruits of Angelica dahurica, in tumor necrosis factor-α (TNF-α)-stimulated HeLa cells by investigating its impact on the production and expression of cytokines and the major signal-transduction pathways. We found this compound significantly inhibited the TNF-α-induced expression of NF-κB target genes, such as COX-2, cyclin  D1, MMP-9, VEGF, IL-6 and Bcl-xL in a concentration-dependent manner...
April 19, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28428059/the-decreased-growth-performance-and-impaired-immune-function-and-structural-integrity-by-dietary-iron-deficiency-or-excess-are-associated-with-tor-nf-%C3%AE%C2%BAb-p38mapk-nrf2-and-mlck-signaling-in-head-kidney-spleen-and-skin-of-grass-carp-ctenopharyngodon-idella
#3
Yan-Lin Guo, Wei-Dan Jiang, Pei Wu, Yang Liu, Xiao-Qiu Zhou, Sheng-Yao Kuang, Ling Tang, Wu-Neng Tang, Yong-An Zhang, Lin Feng
This study was conducted to investigate the effects of dietary iron on the growth, and immune function and structural integrity in head kidney, spleen and skin as well as the underlying signaling of young grass carp (Ctenopharyngodon idella). Total 630 grass carp (242.32 ± 0.58 g) were fed diets containing graded levels of iron at 12.15 (basal diet), 35.38, 63.47, 86.43, 111.09, 136.37 mg/kg (diets 2-6 were added with ferrous fumarate) and 73.50 mg/kg (diet 7 was added with ferrous sulfate) diet for 60 days...
April 18, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28370894/antrodia-camphorata-attenuates-cigarette-smoke-induced-ros-production-dna-damage-apoptosis-and-inflammation-in-vascular-smooth-muscle-cells-and-atherosclerosis-in-apoe-deficient-mice
#4
Hsin-Ling Yang, Mallikarjuna Korivi, Cheng-Hsien Chen, Wei-Jung Peng, Chee-Shan Chen, Mei-Ling Li, Li-Sung Hsu, Jiunn-Wang Liao, You-Cheng Hseu
Cigarette smoke exposure activates several cellular mechanisms predisposing to atherosclerosis, including oxidative stress, dyslipidemia, and vascular inflammation. Antrodia camphorata, a renowned medicinal mushroom in Taiwan, has been investigated for its antioxidant, anti-inflammatory, and antiatherosclerotic properties in cigarette smoke extracts (CSE)-treated vascular smooth muscle cells (SMCs), and ApoE-deficient mice. Fermented culture broth of Antrodia camphorata (AC, 200-800 µg/mL) possesses effective antioxidant activity against CSE-induced ROS production...
April 3, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28366952/oxidative-stress-and-apotosis-to-zebrafish-danio-rerio-embryos-exposed-to-perfluorooctane-sulfonate-pfos-and-zno-nanoparticles
#5
Jia Du, Jun Cai, Shutao Wang, Hong You
OBJECTIVES: Perfluorooctane sulfonate (PFOS) and zinc oxide nanoparticles (ZnO-NPs) are frequently detected in the environment but few studies have assessed the joint toxicity of them. Oxidative stress and apoptosis to zebrafish (Danio rerio) embryos induced by the PFOS and ZnO-NPs were investigated in this study. MATERIAL AND METHODS: The embryos were exposed to the PFOS (0, 0.4, 0.8 and 1.6 mg/l), ZnO-NPs (12.5, 25, 50 mg/l) and PFOS plus ZnO-NPs (0.4+12.5, 0...
March 30, 2017: International Journal of Occupational Medicine and Environmental Health
https://www.readbyqxmd.com/read/28352125/hydrogen-sulfide-ameliorates-chronic-renal-failure-in-rats-by-inhibiting-apoptosis-and-inflammation-through-ros-mapk-and-nf-%C3%AE%C2%BAb-signaling-pathways
#6
Dongdong Wu, Ning Luo, Lianqu Wang, Zhijun Zhao, Hongmin Bu, Guoliang Xu, Yongjun Yan, Xinping Che, Zhiling Jiao, Tengfu Zhao, Jingtao Chen, Ailing Ji, Yanzhang Li, Garrick D Lee
Chronic renal failure (CRF) is a major public health problem worldwide. Hydrogen sulfide (H2S) plays important roles in renal physiological and pathophysiological processes. However, whether H2S could protect against CRF in rats remains unclear. In this study, we found that H2S alleviated gentamicin-induced nephrotoxicity by reducing reactive oxygen species (ROS)-mediated apoptosis in normal rat kidney-52E cells. We demonstrated that H2S significantly improved the kidney structure and function of CRF rats. We found that H2S decreased the protein levels of Bax, Caspase-3, and Cleaved-caspase-3, but increased the expression of Bcl-2...
March 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28323898/perfluorocarbon-reduces-cell-damage-from-blast-injury-by-inhibiting-signal-paths-of-nf-%C3%AE%C2%BAb-mapk-and-bcl-2-bax-signaling-pathway-in-a549-cells
#7
Zhaorui Zhang, Zhixin Liang, Huaidong Li, Chunsun Li, Zhen Yang, Yanqin Li, Danyang She, Lu Cao, Wenjie Wang, Changlin Liu, Liangan Chen
BACKGROUND AND OBJECTIVE: Blast lung injury is a common type of blast injury and has very high mortality. Therefore, research to identify medical therapies for blast injury is important. Perfluorocarbon (PFC) is used to improve gas exchange in diseased lungs and has anti-inflammatory functions in vitro and in vivo. The aim of this study was to determine whether PFC reduces damage to A549 cells caused by blast injury and to elucidate its possible mechanisms of action. STUDY DESIGN AND METHODS: A549 alveolar epithelial cells exposed to blast waves were treated with and without PFC...
2017: PloS One
https://www.readbyqxmd.com/read/28288411/vitexin-alleviates-ox-ldl-mediated-endothelial-injury-by-inducing-autophagy-via-ampk-signaling-activation
#8
Shaoli Zhang, Changlei Guo, Zhigang Chen, Peiyong Zhang, Jianhua Li, Yan Li
Endothelial cell injury plays a crucial role in the development and pathogenesis of cardiovascular disease. Vitexin is a natural flavonoid characterized by anti-oxidative and anti-inflammatory properties. The purpose of this study was to investigate the effects of vitexin on ox-LDL-induced endothelial dysfunction and to explore the underlying molecular mechanisms. In the present study, vitexin was found to play a protective role against ox-LDL-induced endothelial injury. Vitexin significantly promoted cell viability and inhibited apoptosis in ox-LDL-treated HUVECs...
March 10, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28257878/the-carboxyl-terminus-of-heat-shock-protein-70-interacting-protein-chip-participates-in-high-glucose-induced-cardiac-injury
#9
Wenjun Xiong, Shiwen Liu, Wenyao Cai, Jinhua Wen, Yongnan Fu, Jingtian Peng, Zeqi Zheng
The carboxyl terminus of heat shock protein 70-interacting protein (CHIP) is confirmed to have a protective effect on the myocardium, but its effect on diabetic cardiomyopathy is unclear. Small interfering RNA (siRNA) was used for knockdown experiments in neonatal rat cardiomyocytes to examine the function of CHIP in high glucose-induced injury. High glucose stimulated the production of reactive oxygen species (ROS), nicotinamide adenine dinucleotide phosphate oxidase (NOX), interleukin-1β (IL-1β), interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1) production...
February 28, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28245385/-isoalantolactone-inhibits-proliferation-of-k562-a02-cells-through-caspase-dependent-apoptotic-pathway
#10
Yong-Hai Guan, Xiao-Dan Zhang, Hong Cai, Chun-Hui Yang
OBJECTIVE: To explore the apoptosis-inducing effects of isoalantolactone on chronic myeloid leukemia drug-resistant cell line K562/A02. METHODS: K562/A02 cells were treated with 0, 6.25, 12.5, 25, 50, and 100 µmol/L isoalantolactone for 12 h, 24 h, and 48 h. The cell viability was analyzed with CCK8 assay. The effects of isoalantolactone on mitochondrial membrane potential (MMP), reactive oxygen species(ROS) and apoptosis of K562/A02 cells were measured by flow cytometry...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28219713/effect-of-doxorubicin-induced-ovarian-toxicity-on-mouse-ovarian-granulosa-cells
#11
Ting Zhang, Wan Hong He, Ling Lin Feng, Hao Guang Huang
The objective of this study was to identify the effect of doxorubicin-induced ovarian toxicity on mouse ovarian granulosa cells. After granulosa cells were treated with doxorubicin at the final concentrations of 0, 0.4, 0.8, and 1.6 μg/ml for 24 h, cell apoptosis was detected by DAPI staining or caspase-3/7 fluorescence probe; ROS was determined by 2', 7'-dichlorodihydrofluorescin diacetate fluorescence probe; mitochondrial membrane potential was detected by rhodamine-123 fluorescence probe; and mRNA expression levels of Bax, Bcl-2, p53, FSHR, StAR, P450scc and P450arom were analyzed by RT-PCR...
February 17, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28216619/15-deoxy-%C3%AE-12-14-prostaglandin-j2-alleviates-hepatic-ischemia-reperfusion-injury-in-mice-via-inducing-antioxidant-response-and-inhibiting-apoptosis-and-autophagy
#12
Kan Chen, Jing-Jing Li, Sai-Nan Li, Jiao Feng, Tong Liu, Fan Wang, Wei-Qi Dai, Yu-Jing Xia, Jie Lu, Ying-Qun Zhou, Chuan-Yong Guo
Hepatic ischemia-reperfusion (I/R) injury is a common clinical impairment that occurs in many circumstances and leads to poor prognosis. Both apoptosis and autophagy have been shown to contribute to cell death in hepatic I/R injury. 15-Deoxy-Δ(12,14)-prostaglandin J2 (15d-PGJ2) is one of the best-studied anti-inflammatory prostaglandins, which has been verified to exert anti-inflammatory and cell-protective functions in various types of cells and animal models. In this study we explored the effects of 15d-PGJ2 on both apoptosis and autophagy in mouse hepatic I/R injury and its possible mechanisms...
February 20, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28178868/low-level-laser-effect-on-proliferation-migration-and-antiapoptosis-of-mesenchymal-stem-cells
#13
Kan Yin, Rongjia Zhu, Shihua Wang, Robert Chunhua Zhao
Mesenchymal stem cells (MSCs) have been proved to be an important element in cell-based therapy. Photobiomodulation used extremely low-level lasers (LLLs) to affect the behavior of cells. The effect mechanism of LLLs on MSCs from human remained to be discovered. In this study, cell viability was assessed using MTS assays and cell cycle was evaluated by fluorescence-activated cell sorting (FACS). The influence of LLLs on mitochondrial biogenesis (fission or fusion) and function (ATP, reactive oxygen species [ROS], nitric oxide [NO]) was evaluated by transmission electron microscope, FACS, quantitative real time polymerase chain reaction (q-PCR), and immunocytochemistry...
March 27, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28177678/protective-mechanisms-of-melatonin-against-hydrogen-peroxide-induced-toxicity-in-human-bone-marrow-derived-mesenchymal-stem-cells
#14
Saeed Mehrzadi, Majid Safa, Seyed Kamran Kamrava, Radbod Darabi, Parisa Hayat, Manijeh Motevalian
Many obstacles compromise the efficacy of bone marrow mesenchymal stem cells (MSCs) by inducing apoptosis in the grafted MSCs. Current study investigates the effect of melatonin on important mediators involved in survival of BM-MSCs in hydrogen peroxide (H2O2) apoptosis model.In brief, BM-MSCs were isolated treated with melatonin and then exposed to H2O2.Their viability was assessed by MTT assay and apoptotic fractions were evaluated through Annexin V, Hoechst staining and ADP/ATP ratio. Oxidative stress biomarkers including: ROS, total antioxidant power (TAP), superoxide dismutase (SOD) and catalase (CAT) activity, glutathione (GSH), thiol molecules and lipid peroxidation (LPO) levels were determined...
December 23, 2016: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28123512/sirt4-overexpression-protects-against-diabetic-nephropathy-by-inhibiting-podocyte-apoptosis
#15
Jian-Xia Shi, Qi-Jin Wang, Hui Li, Qin Huang
Diabetic nephropathy is a diabetic complication associated with capillary damage and increased mortality. Sirtuin 4 (SIRT4) plays an important role in mitochondrial function and the pathogenesis of metabolic diseases, including aging kidneys. The aim of the present study was to investigate the association between SIRT4 and diabetic nephropathy in a glucose-induced mouse podocyte model. A CCK-8 assay showed that glucose simulation significantly inhibited podocyte proliferation in a time- and concentration-dependent manner...
January 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28068628/epoxy-clerodane-diterpene-inhibits-mcf-7-human-breast-cancer-cell-growth-by-regulating-the-expression-of-the-functional-apoptotic-genes-cdkn2a-rb1-mdm2-and-p53
#16
P Subash-Babu, Ghedeir M Alshammari, S Ignacimuthu, Ali A Alshatwi
Systematic analyses of plants that are used in traditional medicine may lead to the discovery of novel cytotoxic secondary metabolites. Diterpene possesses multiple bioactivities; here, epoxy clerodane diterpene (ECD) was isolated from Tinospora cordifolia (Willd.) stem and shown potential antiproliferative effect in MCF-7 human breast cancer cells. The antiproliferative effect of ECD on MCF-7 cells was systematically analyzed by cell and nuclear morphology, alterations in oxidative stress, and the expression of tumor suppressor and mitochondria-mediated apoptosis-related genes...
March 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28054986/d-l-sulforaphane-induces-ros-dependent-apoptosis-in-human-gliomablastoma-cells-by-inactivating-stat3-signaling-pathway
#17
Ziwei Miao, Fei Yu, Yahao Ren, Jun Yang
d,l-Sulforaphane (SFN), a synthetic analogue of broccoli-derived isomer l-SFN, exerts cytotoxic effects on multiple tumor cell types through different mechanisms and is more potent than the l-isomer at inhibiting cancer growth. However, the means by which SFN impairs glioblastoma (GBM) cells remains poorly understood. In this study, we investigated the anti-cancer effect of SFN in GBM cells and determined the underlying molecular mechanisms. Cell viability assays, flow cytometry, immunofluorescence, and Western blot results revealed that SFN could induced apoptosis of GBM cells in a dose- and time-dependent manner, via up-regulation of caspase-3 and Bax, and down-regulation of Bcl-2...
January 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28011381/induction-of-g2-m-phase-arrest-and-apoptosis-by-a-new-tetrahydroingenol-diterpenoid-from-euphorbia-erythradenia-bioss-in-melanoma-cancer-cells
#18
Faranak Fallahian, Mustafa Ghanadian, Mahmoud Aghaei, Seyed Mohammad Zarei
In the current study, a new tetrahydroingenol diterpene isolated from Euphorbia erythradenia, 7,13-diacetyl-5-angeloyl-20-nicotinyl-3-propionyl-1,2,6,7-tetrahydroingenol (DANPT), were tested for the molecular mechanism of its anti-cancer activity in two human melanoma cancer cell lines, A375 and HMCB. DANPT was found cytotoxic against A375 and HMCB cells with IC50 value of 15.37±2.6μM and 15.62±1.89μM, respectively. Flow cytometric analysis showed that DANPT halted the A375 and HMCB cells in G2/M phase and induced apoptosis in a dose-dependent manner...
February 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27922685/5%C3%A2-bromo%C3%A2-3%C3%A2-3%C3%A2-hydroxyprop%C3%A2-1%C3%A2-ynyl-%C3%A2-2h%C3%A2-pyran%C3%A2-2%C3%A2-one-induces-apoptosis-in-t24-human-bladder-cancer-cells-through-mitochondria-dependent-signaling-pathways
#19
Guo-Qiang Yu, Zhong-Ling Dou, Zhao-Hui Jia
The present study was performed to investigate the effect of 5-bromo-3-(3-hydroxyprop-1-ynyl)-2H-pyran-2-one (BHP) on the induction of apoptosis and cell cycle arrest in T24 human bladder carcinoma cells. An MTT assay was used to investigate the inhibition of cell proliferation. Flow cytometry was used to observe alterations in the cell cycle, generation of reactive oxygen species (ROS), alterations in mitochondrial membrane potential (MMP) and induction of apoptosis in the T24 cells following BHP treatment...
January 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27916261/cis-bifenthrin-causes-immunotoxicity-in-murine-macrophages
#20
Xia Wang, Xingli Gao, Bingnan He, Yuanxiang Jin, Zhengwei Fu
Synthetic pyrethroids (SPs) are commonly used insecticides that have been detected in mammals, including humans, indicating a potential threat to human health. Bifenthrin (BF), as well as other pyrethroids, has been shown to possess neurotoxic, reproductive, hepatotoxic and nephrotoxic potential in mammals. However, studies regarding the immunotoxicity of BF and its mechanism are limited. In this study, we aim to exam the immunotoxicity of cis-BF on the murine macrophage cell line, RAW 264.7. MTT assay results demonstrated that cis-BF exposure induced apoptosis in RAW 264...
February 2017: Chemosphere
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