keyword
MENU ▼
Read by QxMD icon Read
search

virus structural protein

keyword
https://www.readbyqxmd.com/read/28922369/crimean-congo-hemorrhagic-fever-virus-nucleocapsid-protein-has-dual-rna-binding-modes
#1
Subbiah Jeeva, Sean Pador, Brittany Voss, Safder Saieed Ganaie, Mohammad Ayoub Mir
Crimean Congo hemorrhagic fever, a zoonotic viral disease, has high mortality rate in humans. There is currently no vaccine for Crimean Congo hemorrhagic fever virus (CCHFV) and chemical interventions are limited. The three negative sense genomic RNA segments of CCHFV are specifically encapsidated by the nucleocapsid protein into three ribonucleocapsids, which serve as templates for the viral RNA dependent RNA polymerase. Here we demonstrate that CCHFV nucleocapsid protein has two distinct binding modes for double and single strand RNA...
2017: PloS One
https://www.readbyqxmd.com/read/28922109/protein-mediated-rna-folding-governs-sequence-specific-interactions-between-rotavirus-genome-segments
#2
Alexander Borodavka, Eric C Dykeman, Waldemar Schrimpf, Don C Lamb
Segmented RNA viruses are ubiquitous pathogens, which include influenza viruses and rotaviruses. A major challenge in understanding their assembly is the combinatorial problem of a non-random selection of a full genomic set of distinct RNAs. This process involves complex RNA-RNA and protein-RNA interactions, which are often obscured by non-specific binding at concentrations approaching in vivo assembly conditions. Here, we present direct experimental evidence of sequence-specific inter-segment interactions between rotavirus RNAs, taking place in a complex RNA- and protein-rich milieu...
September 18, 2017: ELife
https://www.readbyqxmd.com/read/28921430/production-of-recombinant-rabies-virus-glycoprotein-by-insect-cells-in-a-single-use-fixed-bed-bioreactor
#3
Daniella C Ventini-Monteiro, Renato M Astray, Carlos A Pereira
A single-use fixed-bed bioreactor (iCELLis nano) can be used for cultivating non adherent insect cells, which can be then recovered for scaling up or for harvesting a membrane-associated viral glycoprotein with high quality in terms of preserved protein structure and biological function. Here, we describe the procedures for establishing genetically modified Drosophila melanogaster Schneider 2 (S2) cell cultures in the iCELLis nano bioreactor and for quantifying by ELISA the recombinant rabies virus glycoprotein (rRVGP) synthesized...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28919920/virulence-determinants-of-west-nile-virus-how-can-these-be-used-for-vaccine-design
#4
REVIEW
Jaclyn A Kaiser, Tian Wang, Alan Dt Barrett
West Nile virus (WNV), a neurotropic mosquito-borne flavivirus, has become endemic in the USA and parts of Europe since 1999. There is no licensed WNV vaccine for humans. Considering the robust immunity from immunization with live, attenuated vaccines, a live WNV vaccine is an ideal platform for disease control. Animal and mosquito studies have identified a number of candidate attenuating mutations, including the structural proteins premembrane/membrane and envelope, and the nonstructural proteins NS1, NS2A, NS3, NS4A, NS4B and NS5, and the 3' UTR...
May 2017: Future Virology
https://www.readbyqxmd.com/read/28919883/a-pcr-based-assay-targeting-the-major-capsid-protein-gene-of-a-dinorna-like-ssrna-virus-that-infects-coral-photosymbionts
#5
Jose Montalvo-Proaño, Patrick Buerger, Karen D Weynberg, Madeleine J H van Oppen
The coral-Symbiodinium association is a critical component of coral reefs as it is the main primary producer and builds the reef's 3-dimensional structure. A breakdown of this endosymbiosis causes a loss of the dinoflagellate photosymbiont, Symbiodinium, and/or its photosynthetic pigments from the coral tissues (i.e., coral bleaching), and can lead to coral mortality. Coral bleaching has mostly been attributed to environmental stressors, and in some cases to bacterial infection. Viral lysis of Symbiodinium has been proposed as another possible cause of some instances of coral bleaching, but this hypothesis has not yet been experimentally confirmed...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28919226/validation-of-hav-biomarker-2a-for-differential-diagnostic-of-hepatitis-a-infected-and-vaccinated-individuals-using-multiplex-serology
#6
Katrin Bohm, Angela Filomena, Nicole Schneiderhan-Marra, Gérard Krause, Claudia Sievers
BACKGROUND: Worldwide about 1.5 million clinical cases of hepatitis A virus (HAV) infections occur every year and increasingly countries are introducing HAV vaccination into the childhood immunization schedule with a single dose instead of the originally licenced two dose regimen. Diagnosis of acute HAV infection is determined serologically by anti-HAV-IgM detection using ELISA. Additionally anti-HAV-IgG can become positive during the early phase of symptoms, but remains detectable after infection and also after vaccination against HAV...
September 14, 2017: Vaccine
https://www.readbyqxmd.com/read/28916807/envelope-glycoprotein-mobility-on-hiv-1-particles-depends-on-the-virus-maturation-state
#7
Jakub Chojnacki, Dominic Waithe, Pablo Carravilla, Nerea Huarte, Silvia Galiani, Jörg Enderlein, Christian Eggeling
Human immunodeficiency virus type 1 (HIV-1) assembles as immature particles, which require the proteolytic cleavage of structural polyprotein Gag and the clustering of envelope glycoprotein Env for infectivity. The details of mechanisms underlying Env clustering remain unknown. Here, we determine molecular dynamics of Env on the surface of individual HIV-1 particles using scanning fluorescence correlation spectroscopy on a super-resolution STED microscope. We find that Env undergoes a maturation-induced increase in mobility, highlighting diffusion as one cause for Env clustering...
September 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28916773/functional-organization-of-cytoplasmic-inclusion-bodies-in-cells-infected-by-respiratory-syncytial-virus
#8
Vincent Rincheval, Mickael Lelek, Elyanne Gault, Camille Bouillier, Delphine Sitterlin, Sabine Blouquit-Laye, Marie Galloux, Christophe Zimmer, Jean-François Eleouet, Marie-Anne Rameix-Welti
Infection of cells by respiratory syncytial virus induces the formation of cytoplasmic inclusion bodies (IBs) where all the components of the viral RNA polymerase complex are concentrated. However, the exact organization and function of these IBs remain unclear. In this study, we use conventional and super-resolution imaging to dissect the internal structure of IBs. We observe that newly synthetized viral mRNA and the viral transcription anti-terminator M2-1 concentrate in IB sub-compartments, which we term "IB-associated granules" (IBAGs)...
September 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28916391/zika-virus-structural-biology-and-progress-in-vaccine-development
#9
REVIEW
Hsiao-Han Lin, Bak-Sau Yip, Li-Min Huang, Suh-Chin Wu
The growing number of zika virus (ZIKV) infections plus a 20-fold increase in neonatal microcephaly in newborns in Brazil have raised alarms in many countries regarding the threat to pregnant women. Instances of microcephaly and central nervous system malformations continue to increase in ZIKV outbreak regions. ZIKV is a small enveloped positive-strand RNA virus belonging to the Flavivirus genus of the Flaviviridae family. High-resolution ZIKV structures recently identified by cryo-electron microscopy indicate that the overall ZIKV structure is similar to those of other flaviviruses...
September 12, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28916111/icosahedral-plant-viral-nanoparticles-bioinspired-synthesis-of-nanomaterials-nanostructures
#10
REVIEW
Kannan Badri Narayanan, Sung Soo Han
Viral nanotechnology utilizes virus nanoparticles (VNPs) and virus-like nanoparticles (VLPs) of plant viruses as highly versatile platforms for materials synthesis and molecular entrapment that can be used in the nanotechnological fields, such as in next-generation nanoelectronics, nanocatalysis, biosensing and optics, and biomedical applications, such as for targeting, therapeutic delivery, and non-invasive in vivo imaging with high specificity and selectivity. In particular, plant virus capsids provide biotemplates for the production of novel nanostructured materials with organic/inorganic moieties incorporated in a very precise and controlled manner...
August 31, 2017: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/28915437/temporal-characterization-of-the-non-structural-adenovirus-type-2-proteome-and-phosphoproteome-using-high-resolving-mass-spectrometry
#11
Malin Källsten, Arina Gromova, Hongxing Zhao, Alberto Valdés, Anne Konzer, Ulf Pettersson, Sara Bergström Lind
The proteome and phosphoproteome of non-structural proteins of Adenovirus type 2 (Ad2) were time resolved using a developed mass spectrometry approach. These proteins are expressed by the viral genome and important for the infection process, but not part of the virus particle. We unambiguously confirm the existence of 95% of the viral proteins predicted to be encoded by the viral genome. Most non-structural proteins peaked in expression at late time post infection. We identified 27 non-redundant sites of phosphorylation on seven different non-structural proteins...
September 12, 2017: Virology
https://www.readbyqxmd.com/read/28915104/rift-valley-fever-phlebovirus-nss-protein-core-domain-structure-suggests-molecular-basis-for-nuclear-filaments
#12
Michal Barski, Benjamin Brennan, Ona K Miller, Jane A Potter, Swetha Vijayakrishnan, David Bhella, James H Naismith, Richard M Elliott, Ulrich Schwarz-Linek
Rift Valley fever phlebovirus (RVFV) is a clinically and economically important pathogen increasingly likely to cause widespread epidemics. RVFV virulence depends on the interferon antagonist non-structural protein (NSs), which remains poorly characterized. We identified a stable core domain of RVFV NSs (residues 83-248), and solved its crystal structure, a novel all-helical fold organized into highly ordered fibrils. A hallmark of RVFV pathology is NSs filament formation in infected cell nuclei. Recombinant virus encoding the NSs core domain induced intranuclear filaments, suggesting it contains all essential determinants for nuclear translocation and filament formation...
September 15, 2017: ELife
https://www.readbyqxmd.com/read/28915040/elucidating-the-interdependence-of-drug-resistance-from-combinations-of-mutations
#13
Debra A Ragland, Troy W Whitfield, Sook-Kyung Lee, Ronald Swanstrom, Konstantin B Zeldovich, Nese Kurt Yilmaz, Celia A Schiffer
HIV-1 protease is responsible for the cleavage of 12 non-homologous sites within the Gag and Gag-Pro-Pol polyproteins in the viral genome. Under the selective pressure of protease inhibition, the virus evolves mutations within (primary) and outside of (secondary) the active site allowing the protease to process substrates while simultaneously countering inhibition. The primary protease mutations impede inhibitor binding directly, while the secondary mutations are considered accessory mutations that compensate for a loss in fitness...
September 15, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28912786/co-infection-of-sweet-orange-with-severe-and-mild-strains-of-citrus-tristeza-virus-is-overwhelmingly-dominated-by-the-severe-strain-on-both-the-transcriptional-and-biological-levels
#14
Shimin Fu, Jonathan Shao, Changyong Zhou, John S Hartung
Citrus tristeza is one of the most destructive citrus diseases and is caused by the phloem-restricted Closterovirus, Citrus tristeza virus. Mild strain CTV-B2 does not cause obvious symptoms on indicators whereas severe strain CTV-B6 causes symptoms, including stem pitting, cupping, yellowing, and stiffening of leaves, and vein corking. Our laboratory has previously characterized changes in transcription in sweet orange separately infected with CTV-B2 and CTV-B6. In the present study, transcriptome analysis of Citrus sinensis in response to double infection by CTV-B2 and CTV-B6 was carried out...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28912275/nmr-reveals-the-intrinsically-disordered-domain-2-of-ns5a-protein-as-an-allosteric-regulator-of-the-hepatitis-c-virus-rna-polymerase-ns5b
#15
Luiza M Bessa, Hélène Launay, Marie Dujardin, François-Xavier Cantrelle, Guy Lippens, Isabelle Landrieu, Robert Schneider, Xavier Hanoulle
Non-structural protein 5B (NS5B) is the RNAdependent RNA polymerase that catalyses replication of the hepatitis C virus (HCV) RNA genome, and so is central for its life cycle. NS5B interacts with the intrinsically disordered domain 2 of NS5A (NS5A-D2), another essential multifunctional HCV protein that is required for RNA replication. As a result, these two proteins represent important targets for anti-HCV chemotherapies. Despite this importance and the existence of NS5B crystal structures, our understanding of the conformational and dynamic behaviour of NS5B in solution and its relationship with NS5A-D2 remain incomplete...
September 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28912070/recombinant-influenza-h7-hemagglutinin-containing-cfllc-minidomain-in-the-transmembrane-domain-showed-enhanced-cross-protection-in-mice
#16
Yang Wang, Yun Zhang, Jialing Wu, Ying Lin, Zhihui Wu, Ying Wei, Xiaona Wei, Jianru Qin, Chunyi Xue, George Dacai Liu, Yongchang Cao
Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. The H7N9 viruses are found to diverge into distinct genotypes as other influenza viruses; thus a vaccine that can provide sufficient cross-protection against different genotypes of H7N9 viruses is urgently needed. Our previous studies demonstrated that the HA-based structural design approach by introducing a CFLLC minidomain into transmembrane domain (TM) of H1, H5 or H9 hemagglutinin (HA) proteins by replacing with H3 subtype HA TM could enhance their cross-protection...
September 11, 2017: Virus Research
https://www.readbyqxmd.com/read/28911115/the-ires5-utr-of-the-dicistrovirus-cricket-paralysis-virus-is-a-type-iii-ires-containing-an-essential-pseudoknot-structure
#17
Lauriane Gross, Quentin Vicens, Evelyne Einhorn, Audrey Noireterre, Laure Schaeffer, Lauriane Kuhn, Jean-Luc Imler, Gilbert Eriani, Carine Meignin, Franck Martin
Cricket paralysis virus (CrPV) is a dicistrovirus. Its positive-sense single-stranded RNA genome contains two internal ribosomal entry sites (IRESs). The 5' untranslated region (5'UTR) IRES5'UTR mediates translation of non-structural proteins encoded by ORF1 whereas the well-known intergenic region (IGR) IRESIGR is required for translation of structural proteins from open reading frame 2 in the late phase of infection. Concerted action of both IRES is essential for host translation shut-off and viral translation...
September 6, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28910636/epitranscriptomic-enhancement-of-influenza-a-virus-gene-expression-and-replication
#18
David G Courtney, Edward M Kennedy, Rebekah E Dumm, Hal P Bogerd, Kevin Tsai, Nicholas S Heaton, Bryan R Cullen
Many viral RNAs are modified by methylation of the N(6) position of adenosine (m(6)A). m(6)A is thought to regulate RNA splicing, stability, translation, and secondary structure. Influenza A virus (IAV) expresses m(6)A-modified RNAs, but the effects of m(6)A on this segmented RNA virus remain unclear. We demonstrate that global inhibition of m(6)A addition inhibits IAV gene expression and replication. In contrast, overexpression of the cellular m(6)A "reader" protein YTHDF2 increases IAV gene expression and replication...
September 13, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28910080/cell-surface-interactions-on-arginine-rich-cell-penetrating-peptides-allow-for-multiplex-modes-of-internalization
#19
Shiroh Futaki, Ikuhiko Nakase
One of the recent hot topics in peptide-related chemical biology research is the potential of cell-penetrating peptides (CPPs). Owing to their ability to deliver exogenous molecules into cells easily and effectively, their flexible design that allows transporters to comprise various chemical structures and functions, and their potential in chemical and cell biology studies and clinical applications, CPPs have been attracting enormous interest among researchers in related fields. Consequently, publications on CPPs have increased significantly...
September 14, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28906479/characterization-of-vb_saum-frusau02-a-twort-like-bacteriophage-isolated-from-a-therapeutic-phage-cocktail
#20
Katarzyna Leskinen, Henni Tuomala, Anu Wicklund, Jenni Horsma-Heikkinen, Pentti Kuusela, Mikael Skurnik, Saija Kiljunen
Staphylococcus aureus is a commensal and pathogenic bacterium that causes infections in humans and animals. It is a major cause of nosocomial infections worldwide. Due to increasing prevalence of multidrug resistance, alternative methods to eradicate the pathogen are necessary. In this respect, polyvalent staphylococcal myoviruses have been demonstrated to be excellent candidates for phage therapy. Here we present the characterization of the bacteriophage vB_SauM-fRuSau02 (fRuSau02) that was isolated from a commercial Staphylococcus bacteriophage cocktail produced by Microgen (Moscow, Russia)...
September 14, 2017: Viruses
keyword
keyword
118508
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"