keyword
MENU ▼
Read by QxMD icon Read
search

viral nonstructural protein

keyword
https://www.readbyqxmd.com/read/29097641/computer-aided-analysis-of-phytochemicals-as-potential-dengue-virus-inhibitors-based-on-molecular-docking-admet-and-dft-studies
#1
Iqra Qaddir, Nouman Rasool, Waqar Hussain, Sajid Mahmood
BACKGROUND & OBJECTIVES: Dengue fever, caused by dengue virus (DENV), has become a serious threat to human lives. Phytochemicals are known to have great potential to eradicate viral, bacterial and fungal-borne diseases in human beings. This study was aimed at in silico drug development against nonstructural protein 4B (NS4B) of dengue virus 4 (DENV4). METHODS: A total of 2750 phytochemicals from different medicinal plants were selected for this study. These plants grow naturally in the climate of Pakistan and India and have been used for the treatment of various pathologies in human for long-time...
July 2017: Journal of Vector Borne Diseases
https://www.readbyqxmd.com/read/29077760/membrane-alterations-induced-by-nonstructural-proteins-of-human-norovirus
#2
Sylvie Y Doerflinger, Mirko Cortese, Inés Romero-Brey, Zach Menne, Thibault Tubiana, Christian Schenk, Peter A White, Ralf Bartenschlager, Stéphane Bressanelli, Grant S Hansman, Volker Lohmann
Human noroviruses (huNoV) are the most frequent cause of non-bacterial acute gastroenteritis worldwide, particularly genogroup II genotype 4 (GII.4) variants. The viral nonstructural (NS) proteins encoded by the ORF1 polyprotein induce vesical clusters harboring the viral replication sites. Little is known so far about the ultrastructure of these replication organelles or the contribution of individual NS proteins to their biogenesis. We compared the ultrastructural changes induced by expression of norovirus ORF1 polyproteins with those induced upon infection with murine norovirus (MNV)...
October 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29070684/ns3-from-hcv-strain-jfh-1-is-an-unusually-robust-helicase-that-is-primed-to-bind-and-unwind-viral-rna
#3
Ting Zhou, Xiaoming Ren, Rebecca L Adams, Anna Marie Pyle
Hepatitis C viruses (HCV) encode a helicase enzyme that is essential for viral replication and assembly (NS3). This helicase has become the focus of extensive basic research on general helicase mechanism and it is also of interest as a novel drug target. Despite the importance of this protein, mechanistic work on NS3 has been conducted almost exclusively on variants from HCV genotype 1. Our understanding of NS3 from the highly active HCV strains that are used to study HCV genetics and mechanism in cell culture (such as JFH-1) is lacking...
October 25, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29028178/zika-virus-immune-evasion-mechanisms-currently-available-therapeutic-regimens-and-vaccines
#4
Arun Asif, Sobia Manzoor, Fatima Tuz-Zahra, Muhammad Saalim, Maliha Ashraf, Javeria Ishtiyaq, Madiha Khalid
The sudden emergence of infectious pathogens such as Zika virus (ZIKV) holds global health concerns. Recent dissemination of ZIKV from Pacific to Americas with an upsurge of congenital anomalies and Guillain Barre Syndrome (GBS) in adults has created an alarming situation. High-throughput studies are in progress to understand ZIKV's mode of pathogenesis and mechanism of immune escape, yet the pathogenesis remains obscure. Mainly ZIKV's envelope (E) protein and nonstructural proteins (mainly NS1 and NS5) manipulate host cell to support viral immune escape by modulation of the interferon pathway and complement antagonism...
October 13, 2017: Viral Immunology
https://www.readbyqxmd.com/read/29021567/classical-swine-fever-virus-nonstructural-protein-p7-modulates-infectious-virus-production
#5
Cheng Zhao, Xiaofang Shen, Rui Wu, Ling Li, Zishu Pan
The classical swine fever virus (CSFV) nonstructural protein p7 is crucial for virus production, yet precisely how the p7 modulates this process is unclear. In this study, we first identified the interactions of p7 with E2 and NS2. The key binding regions of both p7 and NS2 mapped to the first transmembrane (TM1) domain of two proteins. Three amino acid substitutions in the TM1 region of p7 (p7(TDI18/19/20AAA), p7(EVV21/22/23AAA) and p7(YFY25/26/30AAA)) impaired infectious virus production and reduced the interaction of p7 with the NS2 protein...
October 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28991178/polyprotein-processing-as-a-determinant-for-in-vitro-activity-of-semliki-forest-virus-replicase
#6
Maija K Pietilä, Irina C Albulescu, Martijn J van Hemert, Tero Ahola
Semliki Forest virus (SFV) is an arthropod-borne alphavirus that induces membrane invaginations (spherules) in host cells. These harbor the viral replication complexes (RC) that synthesize viral RNA. Alphaviruses have four replicase or nonstructural proteins (nsPs), nsP1-4, expressed as polyprotein P1234. An early RC, which synthesizes minus-strand RNA, is formed by the polyprotein P123 and the polymerase nsP4. Further proteolytic cleavage results in a late RC consisting of nsP1-4 and synthesizing plus strands...
October 7, 2017: Viruses
https://www.readbyqxmd.com/read/28988109/nuclear-import-inhibitor-n-4-hydroxyphenyl-retinamide-targets-zika-virus-zikv-nonstructural-protein-5-to-inhibit-zikv-infection
#7
Chunxiao Wang, Sundy N Y Yang, Kate Smith, Jade K Forwood, David A Jans
In the absence of approved therapeutics, Zika virus (ZIKV)'s recent prolific outbreaks in the Americas, together with impacts on unborn fetuses of infected mothers, make it a pressing human health concern worldwide. Although a key player in viral replication in the infected host cell cytoplasm, ZIKV non-structural protein 5 (NS5) appears to contribute integrally to pathogenesis by localising in the host cell nucleus, in similar fashion to NS5 from Dengue virus (DENV). We show here for the first time that ZIKV NS5 is recognized with high nanomolar affinity by the host cell importin α/β1 heterodimer, and that this interaction can be blocked by the novel DENV NS5 targeting inhibitor N-(4-hydroxyphenyl) retinamide (4-HPR)...
December 2, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28978707/prior-dengue-virus-exposure-shapes-t-cell-immunity-to-zika-virus-in-humans
#8
Alba Grifoni, John Pham, John Sidney, Patrick H O'Rourke, Sinu Paul, Bjoern Peters, Sheridan R Martini, Aruna D de Silva, Michael J Ricciardi, Diogo M Magnani, Cassia G T Silveira, Alvino Maestri, Priscilla R Costa, Luzia Maria de-Oliveira-Pinto, Elzinandes Leal de Azeredo, Paulo Vieira Damasco, Elizabeth Phillips, Simon Mallal, Aravinda M de Silva, Matthew Collins, Anna Durbin, Sean A Diehl, Cristhiam Cerpas, Angel Balmaseda, Guillermina Kuan, Josefina Coloma, Eva Harris, James E Crowe, Mars Stone, Phillip J Norris, Michael Busch, Hector Vivanco-Cid, Josephine Cox, Barney S Graham, Julie E Ledgerwood, Lance Turtle, Tom Solomon, Esper G Kallas, David I Watkins, Daniela Weiskopf, Alessandro Sette
While progress has been made in characterizing humoral immunity to Zika virus (ZIKV) in humans, little is known regarding the corresponding T cell responses to ZIKV. Here we investigate the kinetics and viral epitopes targeted by T cells responding to ZIKV and address the critical question of whether pre-existing dengue virus (DENV) T cell immunity modulates these responses. We find that memory T cell responses elicited by prior infection with DENV or vaccination with Tetravalent Dengue Attenuated Vaccines (TDLAV) recognize ZIKV-derived peptides...
October 4, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28977024/rice-stripe-virus-ns3-protein-regulates-primary-mirna-processing-through-association-with-the-mirna-biogenesis-factor-osdrb1-and-facilitates-virus-infection-in-rice
#9
Lijia Zheng, Chao Zhang, Chaonan Shi, Zhirui Yang, Yu Wang, Tong Zhou, Feng Sun, Hong Wang, Shanshan Zhao, Qingqing Qin, Rui Qiao, Zuomei Ding, Chunhong Wei, Lianhui Xie, Jianguo Wu, Yi Li
MicroRNAs (miRNAs) are small regulatory RNAs processed from primary miRNA transcripts, and plant miRNAs play important roles in plant growth, development, and response to infection by microbes. Microbial infections broadly alter miRNA biogenesis, but the underlying mechanisms remain poorly understood. In this study, we report that the Rice stripe virus (RSV)-encoded nonstructural protein 3 (NS3) interacts with OsDRB1, an indispensable component of the rice (Oryza sativa) miRNA-processing complex. Moreover, the NS3-OsDRB1 interaction occurs at the sites required for OsDRB1 self-interaction, which is essential for miRNA biogenesis...
October 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28973992/3d-spatially-resolved-models-of-the-intracellular-dynamics-of-the-hepatitis-c-genome-replication-cycle
#10
Markus M Knodel, Sebastian Reiter, Paul Targett-Adams, Alfio Grillo, Eva Herrmann, Gabriel Wittum
Mathematical models of virus dynamics have not previously acknowledged spatial resolution at the intracellular level despite substantial arguments that favor the consideration of intracellular spatial dependence. The replication of the hepatitis C virus (HCV) viral RNA (vRNA) occurs within special replication complexes formed from membranes derived from endoplasmatic reticulum (ER). These regions, termed membranous webs, are generated primarily through specific interactions between nonstructural virus-encoded proteins (NSPs) and host cellular factors...
September 30, 2017: Viruses
https://www.readbyqxmd.com/read/28968829/the-ns1-protein-of-respiratory-syncytial-virus-blocks-glucocorticoid-receptor-nuclear-translocation-by-targeting-ipo13-may-account-for-glucocorticoid-insensitive
#11
Jun Xie, Xiaoru Long, Leiqiong Gao, Sisi Chen, Keting Zhao, Wei Li, Na Zhou, Na Zang, Yu Deng, Luo Ren, Lijia Wang, Zhengxiu Luo, Wenwei Tu, Xiaodong Zhao, Zhou Fu, Xiaohong Xie, Enmei Liu
In spite of their powerful anti-inflammatory effect, glucocorticoids have shown no significant clinical benefit in respiratory syncytial virus (RSV)-induced bronchiolitis, the reason for which remains unclear. Upon glucocorticoid binding, the cytoplasmic glucocorticoid receptor (GR) translocates to the nucleus with the help of importin-13 (IPO13). Here, we report that RSV infection reduced GR nuclear translocation in the nasopharyngeal aspirate (NPA) of the RSV-infected infants, lungs of infected mice, and A549 cells, which coincided with decreased IPO13 expression...
September 1, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28961161/venezuelan-equine-encephalitis-virus-capsid-the-clever-caper
#12
REVIEW
Lindsay Lundberg, Brian Carey, Kylene Kehn-Hall
Venezuelan equine encephalitis virus (VEEV) is a New World alphavirus that is vectored by mosquitos and cycled in rodents. It can cause disease in equines and humans characterized by a febrile illness that may progress into encephalitis. Like the capsid protein of other viruses, VEEV capsid is an abundant structural protein that binds to the viral RNA and interacts with the membrane-bound glycoproteins. It also has protease activity, allowing cleavage of itself from the growing structural polypeptide during translation...
September 29, 2017: Viruses
https://www.readbyqxmd.com/read/28954927/rapid-antigen-tests-for-dengue-virus-serotypes-and-zika-virus-in-patient-serum
#13
Irene Bosch, Helena de Puig, Megan Hiley, Marc Carré-Camps, Federico Perdomo-Celis, Carlos F Narváez, Doris M Salgado, Dewahar Senthoor, Madeline O'Grady, Elizabeth Phillips, Ann Durbin, Diana Fandos, Hikaru Miyazaki, Chun-Wan Yen, Margarita Gélvez-Ramírez, Rajas V Warke, Lucas S Ribeiro, Mauro M Teixeira, Roque P Almeida, José E Muñóz-Medina, Juan E Ludert, Mauricio L Nogueira, Tatiana E Colombo, Ana C B Terzian, Patricia T Bozza, Andrea S Calheiros, Yasmine R Vieira, Giselle Barbosa-Lima, Alexandre Vizzoni, José Cerbino-Neto, Fernando A Bozza, Thiago M L Souza, Monique R O Trugilho, Ana M B de Filippis, Patricia C de Sequeira, Ernesto T A Marques, Tereza Magalhaes, Francisco J Díaz, Berta N Restrepo, Katerine Marín, Salim Mattar, Daniel Olson, Edwin J Asturias, Mark Lucera, Mohit Singla, Guruprasad R Medigeshi, Norma de Bosch, Justina Tam, Jose Gómez-Márquez, Charles Clavet, Luis Villar, Kimberly Hamad-Schifferli, Lee Gehrke
The recent Zika virus (ZIKV) outbreak demonstrates that cost-effective clinical diagnostics are urgently needed to detect and distinguish viral infections to improve patient care. Unlike dengue virus (DENV), ZIKV infections during pregnancy correlate with severe birth defects, including microcephaly and neurological disorders. Because ZIKV and DENV are related flaviviruses, their homologous proteins and nucleic acids can cause cross-reactions and false-positive results in molecular, antigenic, and serologic diagnostics...
September 27, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28942178/eef1g-interaction-with-foot-and-mouth-disease-virus-nonstructural-protein-2b-identification-by-yeast-two-hybrid-system
#14
Zhongwang Zhang, Li Pan, Yaozhong Ding, Jianliang Lv, Peng Zhou, Yuzhen Fang, Xinsheng Liu, Yongguang Zhang, Yonglu Wang
Foot-and-mouth disease virus (FMDV) is a picornavirus that causes an economically significant disease in cattle and swine. Replication of FMDV is dependent on both viral proteins and cellular factors. Nonstructural protein 2B of FMDV plays multiple roles during viral infection and replication. We investigated the roles of 2B in virus-host interactions by constructing a cDNA library obtained from FMDV-infected swine tissues, and used a split-ubiquitin-based yeast two-hybrid system to identify host proteins that interacted with 2B...
September 21, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28941869/transcription-associated-mutational-pressure-in-the-parvovirus-b19-genome-reactivated-genomes-contribute-to-the-variability-of-viral-populations
#15
Vladislav Victorovich Khrustalev, Marina Anatolyevna Ermalovich, Judith M Hübschen, Tatyana Aleksandrovna Khrustaleva
In this study we used non-overlapping parts of the two long open reading frames coding for nonstructural (NS) and capsid (VP) proteins of all available sequences of the Parvovirus B19 subgenotype 1a genome and found out that the rates of A to G, C to T and A to T mutations are higher in the first long reading frame (NS) of the virus than in the second one (VP). This difference in mutational pressure directions for two parts of the same viral genome can be explained by the fact of transcription of just the first long reading frame during the lifelong latency in nonerythroid cells...
December 21, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28904127/therapeutic-effects-of-monoclonal-antibody-against-dengue-virus-ns1-in-a-stat1-knockout-mouse-model-of-dengue-infection
#16
Shu-Wen Wan, Pei-Wei Chen, Chin-Yu Chen, Yen-Chung Lai, Ya-Ting Chu, Chia-Yi Hung, Han Lee, Hsuan Franziska Wu, Yung-Chun Chuang, Jessica Lin, Chih-Peng Chang, Shuying Wang, Ching-Chuan Liu, Tzong-Shiann Ho, Chiou-Feng Lin, Chien-Kuo Lee, Betty A Wu-Hsieh, Robert Anderson, Trai-Ming Yeh, Yee-Shin Lin
Dengue virus (DENV) is the causative agent of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome and is endemic to tropical and subtropical regions of the world. Our previous studies showed the existence of epitopes in the C-terminal region of DENV nonstructural protein 1 (NS1) which are cross-reactive with host Ags and trigger anti-DENV NS1 Ab-mediated endothelial cell damage and platelet dysfunction. To circumvent these potentially harmful events, we replaced the C-terminal region of DENV NS1 with the corresponding region from Japanese encephalitis virus NS1 to create chimeric DJ NS1 protein...
October 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28893180/annexin-a2-anxa2-interacts-with-nonstructural-protein-1-and-promotes-the-replication-of-highly-pathogenic-h5n1-avian-influenza-virus
#17
Yong Ma, Jiashan Sun, Linlin Gu, Hongmei Bao, Yuhui Zhao, Lin Shi, Wei Yao, Guobin Tian, Xiurong Wang, Hualan Chen
BACKGROUND: Non-structural protein 1 (NS1) is a multifunctional protein and a crucial regulatory factor in the replication and pathogenesis of avian influenza virus (AIV). Studies have shown that NS1 can interact with a variety of host proteins to modulate the viral life cycle. We previously generated a monoclonal antibody against NS1 protein; In the current research study, using this antibody, we immunoprecipitated host proteins that interact with NS1 to better understand the roles played by NS1 in communications between virus and host...
September 11, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28882564/searching-for-synergy-identifying-optimal-antiviral-combination-therapy-using-hepatitis-c-virus-hcv-agents-in-a-replicon-system
#18
Justin J Pomeroy, George L Drusano, Jaime L Rodriquez, Ashley N Brown
Direct acting antiviral agents (DAAs) are potent inhibitors of Hepatitis C virus (HCV) that have revolutionized the treatment landscape for this important viral disease. There are currently four classes of DAAs that inhibit HCV replication via distinct mechanisms of action: nonstructural protein (NS) 3/4a protease inhibitors, NS5A inhibitors, NS5B nucleoside polymerase inhibitors, and NS5B non-nucleoside polymerase inhibitors. Combination therapy with two or more DAAs has great potential to further enhance antiviral potency...
October 2017: Antiviral Research
https://www.readbyqxmd.com/read/28875696/surface-enhanced-raman-spectroscopy-based-sandwich-immunoassays-for-multiplexed-detection-of-zika-and-dengue-viral-biomarkers
#19
Maria Sánchez-Purrà, Marc Carré-Camps, Helena de Puig, Irene Bosch, Lee Gehrke, Kimberly Hamad-Schifferli
Zika and dengue are mosquito-borne diseases that present similar nonspecific symptoms but possess dramatically different outcomes. The first line of defense in epidemic outbreaks are rapid point-of-care diagnostics. Because many outbreaks occur in areas that are resource poor, assays that are easy to use, inexpensive, and require no power have become invaluable in patient treatment, quarantining, and surveillance. Paper-based sandwich immunoassays such as lateral flow assays (LFAs) are attractive as point-of-care solutions as they have the potential for wider deployability than lab-based assays such as PCR...
October 13, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28874549/molecular-basis-of-mammalian-transmissibility-of-avian-h1n1-influenza-viruses-and-their-pandemic-potential
#20
Mark Zanin, Sook-San Wong, Subrata Barman, Challika Kaewborisuth, Peter Vogel, Adam Rubrum, Daniel Darnell, Atanaska Marinova-Petkova, Scott Krauss, Richard J Webby, Robert G Webster
North American wild birds are an important reservoir of influenza A viruses, yet the potential of viruses in this reservoir to transmit and cause disease in mammals is not well understood. Our surveillance of avian influenza viruses (AIVs) at Delaware Bay, USA, revealed a group of similar H1N1 AIVs isolated in 2009, some of which were airborne-transmissible in the ferret model without prior adaptation. Comparison of the genomes of these viruses revealed genetic markers of airborne transmissibility in the Polymerase Basic 2 (PB2), PB1, PB1-F2, Polymerase Acidic-X (PA-X), Nonstructural Protein 1 (NS1), and Nuclear Export Protein (NEP) genes...
October 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
keyword
keyword
118507
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"