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Leukemia plasmatic's cells

Silvia Ravera, Vanessa Cossu, Barbara Tappino, Elena Nicchia, Carlo Dufour, Simona Cavani, Andrea Sciutto, Claudia Bolognesi, Marta Columbaro, Paolo Degan, Enrico Cappelli
Metformin (MET) is the drug of choice for patients with type 2 diabetes and has been proposed for use in cancer therapy and for treating other metabolic diseases. More than 14,000 studies have been published addressing the cellular mechanisms affected by MET. However, several in vitro studies have used concentrations of the drug 10-100-fold higher than the plasmatic concentration measured in patients. Here, we evaluated the biochemical, metabolic, and morphologic effects of various concentrations of MET. Moreover, we tested the effect of MET on Fanconi Anemia (FA) cells, a DNA repair genetic disease with defects in energetic and glucose metabolism, as well as on human promyelocytic leukemia (HL60) cell lines...
February 2018: Journal of Cellular Physiology
Enrique Podaza, Florencia Sabbione, Denise Risnik, Mercedes Borge, María B Almejún, Ana Colado, Horacio Fernández-Grecco, María Cabrejo, Raimundo F Bezares, Analía Trevani, Romina Gamberale, Mirta Giordano
Chronic lymphocytic leukemia (CLL) is characterized by immune defects that contribute to a high rate of infections and autoimmune cytopenias. Neutrophils are the first line of innate immunity and respond to pathogens through multiple mechanisms, including the release of neutrophil extracellular traps (NETs). These web-like structures composed of DNA, histones, and granular proteins are also produced under sterile conditions and play important roles in thrombosis and autoimmune disorders. Here we show that neutrophils from CLL patients are more prone to release NETs compared to those from age-matched healthy donors (HD)...
January 2017: Cancer Immunology, Immunotherapy: CII
Daniel Gonçalves Chaves, Camila Campos Sales, Poliane de Cássia Gonçalves, Maria Clara Fernandes da Silva-Malta, Luiz Cláudio Romanelli, João Gabriel Ribas, Anna Bárbara de Freitas Carneiro-Proietti, Marina Lobato Martins
The human T-cell leukemia virus type 1 (HTLV-1) is present throughout the world and is associated with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and other inflammatory conditions. The pathogenesis of HAM/TSP involves a chronic inflammatory response in central nervous system (CNS), with the presence of HTLV-1 infected cells and HTLV-1-specific CD8+ lymphocytes. Chemokines may have a role in the infiltration of these cells into the CNS. In this context, the present study analyzed the level of plasmatic chemokines CCL2 (MCP-1), CCL5 (RANTES), IL8 (CXCL8), CXCL9 (MIG), and CXCL10 (IP-10) and HTLV-1 proviral load from peripheral blood in 162 asymptomatic carriers and 136 HAM/TSP patients to determine the differences that be associated with the clinical status of the HTLV-1 infection...
August 2016: Journal of Medical Virology
Débora S S Costa, Thiago Martino, Fernanda C Magalhães, Graça Justo, Marsen G P Coelho, Julio C F Barcellos, Victor B Moura, Paulo R R Costa, Kátia C C Sabino, Ayres G Dias
New O-isoprenylated-N-methylarylnitrones derived from isomeric o, m and p-hydroxybenzaldehydes have been prepared and the antineoplastic effects on human cancer cell lines were evaluated. The O-geranylated nitrone LQB-278 (1b) and its isomers 2b and 3b inhibited the NO production, but the anti-leukemic activity was drastically dependent on nitrone isomer, with the 1b being the most effective one (IC₅₀ of 6.7 μM) on Jurkat leukemia cell, by MTT assay. In addition, 1b up-regulated p21CIP1/WAF1/Sdi1 protein expression (flow cytometry), a cell cycle inhibitor, reduced cell growth, and induced DNA fragmentation (increased sub-G1 phase cells) and phosphatidylserine externalization in plasmatic membrane (increased annexin V positive cells)...
May 1, 2015: Bioorganic & Medicinal Chemistry
Veronica Carnicelli, Anna Rita Lizzi, Giancaterino Gualtieri, Argante Bozzi, Nicola Franceschini, Antonio Di Giulio
Azidothymidine (AZT) is one of the anti-retroviral drugs currently used for the treatment of HIV-infected patients. Several other effects of the drug have been studied in vitro, such as the alterations of some enzymes, the inhibition of cell proliferation, and the increase of transferrin receptor expression. In this study, we investigated the alterations of protein kinase C (PKC) activity, PKCα and PKCβII expressions and plasmatic membrane fluidity induced by AZT in two cancer cell lines, human chronic myeloid (K562) and human acute lymphoblastic (HSB-2) leukemia cells, respectively...
April 2015: Acta Biochimica et Biophysica Sinica
Dayanne da Silva Borges Betiati, Paula Fernanda de Oliveira, Carolina de Quadros Camargo, Everson Araújo Nunes, Erasmo Benício Santos de Moraes Trindade
The development of leukemia and lymphomas is related to the increase in inflammatory process modulators. These, in turn, have divergent actions on the neoplastic process. Populations of T cells have different roles in the neoplastic environment; while interferon-gamma positive T cells have antitumor activity, the FoxP3+interleukin-10 positive population present a pro-tumor activity. Simultaneously, the inflammatory process promotes the mobilization of fatty acids from the cell membrane to produce lipid mediators, which also participate of the inflammatory response...
2013: Revista Brasileira de Hematologia e Hemoterapia
Elodie Ducros, Shah Soltan Mirshahi, Anne-Marie Faussat, Pezhman Mirshahi, Sophie Dimicoli, Ruoping Tang, Julia Pardo, Jdid Ibrahim, Jean-Pierre Marie, Amu Therwath, Jeannette Soria, Massoud Mirshahi
Elevated plasma level of soluble endothelial protein C receptor (sEPCR) may be an indicator of thrombotic risk. The present study aims to correlate leukemia-associated hypercoagulability to high level plasma sEPCR and proposes its measurement in routine clinical practice. EPCR expressions in leukemic cell lines were determined by flow cytometry, immunocytochemistry, and reverse transcription polymerase chain reaction (RT-PCR). EPCR gene sequence of a candidate cell line HL-60 was also determined. Plasma samples (n = 76) and bone marrow aspirates (n = 72) from 148 patients with hematologic malignancies and 101 healthy volunteers were analyzed by enzyme-linked immunosorbent assay (ELISA) via a retrospective study for sEPCR and D-dimer...
October 2012: Cancer Medicine
Rim Charfi, Sameh Trabelsi, Issam Salouage, Emna Gaïes, Nadia Jebabli, Mohamed Lakhal, Anis Klouz
Methotrexate is an antifolate drug used intravenously at high-dose in acute lymphocytic leukemia (ALL). Therapeutic drug monitoring is required to identify patients at risk of developing toxicity and to control folinic acid rescue. We report a case of Münchausen syndrome by proxy revealed by high and persistent falsely toxic methotrexate plasmatic levels. A 12 year-old child was treated with chemotherapy including methotrexate every 70 days for an ALL. The last methotrexate plasmatic level was 0.15 μmol/L at the 72th hour of the infusion...
March 2012: Annales de Biologie Clinique
N Selvy, M Villiet, S Hansel-Esteller
UNLABELLED: OBJECTIVE OF THIS STUDY: The mortality level of leucemic patient's fungal infections can reach 70%. Antifungal prophylaxis posaconazole (P) is used during severe and prolonged neutropenia resulting from chemotherapy for acute myelogenous leukemia (AML), myelodysplastic syndrome and after autologous transplant. PATIENTS AND METHODS: We realized a retrospective study in order to assess the efficiency of P. Oral prophylaxy dosage is 200mg every eight hours to beginning before presumed neutropenia...
April 2010: Pathologie-biologie
Martha C Domínguez, Miyerlandi Torres, Oscar Tamayo, William Criollo, Milton Quintana, Adalberto Sánchez, Felipe García
INTRODUCTION: Previous reports have given evidence that in tropical spastic paraparesis (TSP)/human T-lymphotrophic virus (HTLV-I)-associated myelopathy (HAM), an autoimmune process occurs as part of its pathogenesis. OBJECTIVE: The roles of autoimmunity and the molecular mimicry was evaluated in TSP/HAM patients. MATERIALS AND METHODS: Plasma samples were characterized from patients in the Pacific coastal region of Colombia. Thirty-seven were identified as TSP/HAM, 10 were diagnosed with adult T-cell leukemia virus, 22 were asymptomatic carriers but seropositive for HTLV-I and 20 were seronegative and served as negative controls...
December 2008: Biomédica: Revista del Instituto Nacional de Salud
Ettore Biagi, Marina Col, Maddalena Migliavacca, Mariagrazia Dell'Oro, Daniela Silvestri, Alessandro Montanelli, Giuseppe Peri, Alberto Mantovani, Andrea Biondi, Mario Renato Rossi
Pentraxin-3 (PTX3) is a member of the long pentraxin superfamily and has a nonredundant role in mediating resistance to fungal pathogens. Serial monitoring of PTX3 plasmatic levels was performed in 10 pediatric leukemia patients affected by pulmonary fungal infections. When compared with values of a control pediatric cohort, PTX3 showed significantly higher plasmatic values. Moreover, the response to the antifungal therapy correlated with normalization of PTX3 values. PTX3 may represent a useful tool for the diagnosis and monitoring of fungal infections in immuno-compromised children...
December 2008: Journal of Pediatric Hematology/oncology
Vanessa Battisti, Liési D K Maders, Margarete D Bagatini, Karen F Santos, Rosélia M Spanevello, Paula A Maldonado, Alice O Brulé, Maria do Carmo Araújo, Maria R C Schetinger, Vera M Morsch
OBJECTIVES: To evaluate the oxidative status and antioxidant defense in patients with acute lymphoblastic leukemia (ALL). DESIGN AND METHODS: We measured concentrations of plasmatic thiobarbituric acid reactive substances (TBARS), serum protein carbonylation, whole blood catalase (CAT) and superoxide dismutase (SOD) activities, as well as the plasmatic and erythrocyte thiol levels and serum vitamin E concentration. This study was performed on 80 children with ALL divided into 4 groups: just diagnosed, remission induction, remission maintenance and out-of-treatment...
May 2008: Clinical Biochemistry
Mario Rende, Alessandra Pistilli, Anna Maria Stabile, Adelmo Terenzi, Antonino Cattaneo, Gabriele Ugolini, Pietro Sanna
Neurotrophins, originally identified as neuronal survival and differentiation factors, exert their actions through tyrosine kinase receptors such as TrKA, in the case of the nerve growth factor. Neurotrophins also interact with p75, a common receptor devoid of kinase activity and connected to apoptosis. Here we show that nerve growth factor, TrKA and p75 are expressed in cell lines of human cancers of various non-neuronal lineages, including a panel of muscular sarcomas, and we show that all cell lines investigated actively release nerve growth factor into the medium...
September 2006: Anti-cancer Drugs
J-L Orsonneau, E A Brassart, M Lecame, P Thomare, O Delaroche, D Dudouet
The L-asparaginase is a critical drug for the treatment of acute lymphoblastic leukaemia, that achieves blood L-asparagin depletion. However, such a therapy is associated with a high rate of negative side effects, particularly antibody synthesis against L-asparaginase. This therefore decreases therapy efficiency requiring the monitoring of L-asparaginase activity since L-asparagin determination is not easy. We compared here the results obtained with an automated kinetic enzymatic method to those obtained with the most commonly used Nessler reagent method...
September 2004: Annales de Biologie Clinique
N Boissel, P Rousselot, E Raffoux, J-M Cayuela, O Maarek, D Charron, L Degos, H Dombret, A Toubert, D Rea
Human blood dendritic cells (DC) comprise plasmacytoid DC (PDC) and myeloid DC (MDC), which both prime antitumor T-cell responses. We prospectively monitored blood DC in 30 chronic myeloid leukemia (CML) patients before and after imatinib mesylate therapy. We found a dramatic reduction in PDC and MDC prior treatment. This reduction was associated with high plasmatic vascular endothelial growth factor (VEGF), a central regulator of angiogenesis which also participates to tumor-associated immune deficiencies...
October 2004: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
Yasmine Sebti, Gaëlle Le Friec, Céline Pangault, Frédéric Gros, Bernard Drénou, Valérie Guilloux, Marc Bernard, Thierry Lamy, Renée Fauchet, Laurence Amiot
The immunomodulatory properties of soluble human leukocyte antigen G (sHLA-G) explain its potential interest in malignancies. HLA-G frequently transcribed in lymphoproliferative disorders is rarely expressed at cell surface. In this article, we will demonstrate that the plasmatic level of soluble HLA-G was significantly increased in 70% of B chronic lymphocytic leukemia, 53% of non-Hodgkin B lymphoma (B-NHL), and 45% of T-NHL. To explain this variable secretion, the HLA-G secreting cell was searched and was identified as tumoral T4 lymphocytes only in one patient with Sezary syndrome...
November 2003: Human Immunology
Julio J Criado, Juan L Manzano, E Rodríguez-Fernández
Based on the ability of bile acids to vectorialize the cytostatic activity of other agents, we have designed and synthesized a new series of platinum and gold complexes. These compounds were studied and characterized by elemental analysis, FT-IR, FAB(+)/MS, 1H, 13C and 195Pt NMR, UV-Vis spectroscopy and conductivity measurements in solution, among other techniques. Kinetic studies carried out in aqueous solution and in the presence of different NaCl concentrations: 4 mM (similar to cytoplasmic concentration), 150 mM (similar to plasmatic concentration)...
August 1, 2003: Journal of Inorganic Biochemistry
I Dovinová, H Paulíková, P Rauko, L' Hunáková, E Hanusovská, E Tibenská
Several metal complex agents have already been introduced into clinical tumor therapy and others are subject of antitumor studies. In this study we focused on the tetraaza macrocyclic copper complex (Cu(TAAB)Cl(2)). We studied the influence of the substance on cell growth, cell cycle, membrane integrity, necrosis, apotosis and glutathione level on the leukemic cell line L1210 in 1-day (22 h) and 3-day (72 h) experiments. The metal complex shows a dose-dependent antiproliferative effect, without affecting cell cycle phases...
October 2002: Toxicology in Vitro: An International Journal Published in Association with BIBRA
F Pea, D Russo, M Michieli, M Baraldo, A Ermacora, D Damiani, M Baccarani, M Furlanut
Liposomal formulations of anthracyclines have been developed to increase their delivery to solid tumors while reducing toxicity in normal tissues. DaunoXome (DNX, NeXstar) is a liposomal-encapsulated preparation of daunorubicin registered for treatment of Kaposi's sarcoma that during prior in vitro studies showed a toxicity to leukemic cells at least comparable to that of free daunorubicin. The aim of our study was to determine DNX pharmacokinetics in 11 poor-risk patients with acute leukemia treated with DNX 60 mg/m2 IV on days 1, 3, and 5...
2000: Cancer Chemotherapy and Pharmacology
L M Isakova, N N Tretiak, V N Khromiak, A I Gordienko, A S Zverkova, A M Vakul'chuk
The results of study of morpho-cytochemical peculiarities, antigenic profile and peripheric blood and/or bone marrow blast cell karyotype of 21 patients with acute myeloid leukemia are presented. Hemopoietic cell immunophenotyping was carried out with the use of cytofluorometer FACScan and chromosome cytogenetic analysis with the use of analyzer "Metascan". It has been shown that in the AML M1 blast cell plasmatic membrane carries pan-myeloid CD33 and CD13 antigens, the last having high density of expression, and the CD38 antigen, which is a myeloid cell-precursor marker...
January 2000: T︠S︡itologii︠a︡ i Genetika
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