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Hao Duan, Xu Zhang, Fei-Xiang Wang, Mu-Yan Cai, Guo-Wei Ma, Hong Yang, Jian-Hua Fu, Zi-Hui Tan, Xia-Yu Fu, Qi-Long Ma, Xin-Ye Wang, Peng Lin
To determine the prognostic significance of Kinesin family member 2C (KIF-2C) expression in patients with operable esophageal squamous cell carcinoma (ESCC), we conducted an immunohistochemical analysis of KIF-2C expression in 415 surgically resected primary tumor tissues and 40 adjacent non-cancerous tissues from patients with operable ESCC. The median duration of postoperative follow-up was 76.0 months. Higher KIF-2C expression was associated with significantly increased risks of higher pathologic tumor (pT) status (P=0...
August 22, 2016: Oncotarget
Wu-Lin Charng, Ender Karaca, Zeynep Coban Akdemir, Tomasz Gambin, Mehmed M Atik, Shen Gu, Jennifer E Posey, Shalini N Jhangiani, Donna M Muzny, Harsha Doddapaneni, Jianhong Hu, Eric Boerwinkle, Richard A Gibbs, Jill A Rosenfeld, Hong Cui, Fan Xia, Kandamurugu Manickam, Yaping Yang, Eissa A Faqeih, Ali Al Asmari, Mohammed A M Saleh, Ayman W El-Hattab, James R Lupski
BACKGROUND: Neurodevelopment is orchestrated by a wide range of genes, and the genetic causes of neurodevelopmental disorders are thus heterogeneous. We applied whole exome sequencing (WES) for molecular diagnosis and in silico analysis to identify novel disease gene candidates in a cohort from Saudi Arabia with primarily Mendelian neurologic diseases. METHODS: We performed WES in 31 mostly consanguineous Arab families and analyzed both single nucleotide and copy number variants (CNVs) from WES data...
2016: BMC Medical Genomics
Wen-Chun Liu, Yu-Li Lin, Maureen Spearman, Pei-Yun Cheng, Michael Butler, Suh-Chin Wu
UNLABELLED: Influenza virus hemagglutinin (HA) N-glycans play important regulatory roles in the control of virus virulence, antigenicity, receptor-binding specificity, and viral escape from the immune response. Considered essential for controlling innate and adaptive immune responses against influenza virus infections, dendritic cells (DCs) trigger proinflammatory and adaptive immune responses in hosts. In this study, we engineered Chinese hamster ovary (CHO) cell lines expressing recombinant HA from pandemic H1, H5, and H7 influenza viruses...
July 1, 2016: Journal of Virology
Xue Qiao, Ru Li, Wei Song, Wen-juan Miao, Jia Liu, Hu-biao Chen, De-an Guo, Min Ye
Structural identification of natural products by tandem mass spectrometry requires laborious spectral analysis. Herein, we report a targeted post-acquisition data processing strategy, key ion filtering (KIF), to analyze untargeted mass spectral data. This strategy includes four steps: (1) untargeted data acquisition by ultra-high performance liquid chromatography coupled with hybrid quadrupole orbitrap mass spectrometry (UHPLC/orbitrap-MS); (2) construction of a key ion database according to diagnostic MS/MS fragmentations and conservative substructures of natural compounds; (3) high-resolution key ion filtering of the acquired data to recognize substructures; and (4) structural identification of target compounds by analyzing their MS/MS spectra...
April 8, 2016: Journal of Chromatography. A
Shuijie Li, Stuart M Fell, Olga Surova, Erik Smedler, Karin Wallis, Zhi Xiong Chen, Ulf Hellman, John Inge Johnsen, Tommy Martinsson, Rajappa S Kenchappa, Per Uhlén, Per Kogner, Susanne Schlisio
KIF1Bβ is a candidate 1p36 tumor suppressor that regulates apoptosis in the developing sympathetic nervous system. We found that KIF1Bβ activates the Ca(2+)-dependent phosphatase calcineurin (CN) by stabilizing the CN-calmodulin complex, relieving enzymatic autoinhibition and enabling CN substrate recognition. CN is the key mediator of cellular responses to Ca(2+) signals and its deregulation is implicated in cancer, cardiac, neurodegenerative, and immune disease. We show that KIF1Bβ affects mitochondrial dynamics through CN-dependent dephosphorylation of Dynamin-related protein 1 (DRP1), causing mitochondrial fission and apoptosis...
January 25, 2016: Developmental Cell
J A Hubacek, M Vrablik, D Dlouha, V Stanek, M Gebauerova, V Adamkova, R Ceska, G Dostálová, A Linhart, L Vitek, J Pitha
AIM: Cardiovascular disease (CVD) is a major cause of morbidity and mortality in developed countries. This study aimed to confirm the effect of common putative CVD-associated gene variants (FTO rs17817449, KIF6 rs20455, 9p21 rs10757274 and 2q36.3 rs2943634) on CVD manifestation, and determine whether this effect differs between younger (< 50 years) and older CVD patients. METHODS: 1191 controls and 1889 MI patients were analyzed. All participants were Caucasian Czech males aged <65 years (532 were <50 years) who were examined at cardiology clinics in Prague, Czech Republic...
February 15, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
Abigail U Emelonye, Taina Pitkäaho, Katri Vehviläinen-Julkunen
Background. Measures of spousal effect during parturient pain should take a tripartite approach involving the parturients, spouses, and midwives. Aim. To develop and validate three questionnaires measuring spousal presence in management of parturient pain in Nigeria. Methods. There are two phases: (1) development of questionnaires, Abuja Instrument for Midwives (AIM), Abuja Instrument for Parturient Pain (AIPP), and Abuja Instrument for Parturient Spouses (AIPS), utilizing literatures, Kuopio instrument for fathers (KIF) and expertise of health professionals, and (2) pilot study to validate the questionnaires which were administered in two hospitals in Nigeria: midwives (n = 10), parturients (n = 10), and spouses (n = 10)...
2015: Nursing Research and Practice
Vinod Kumar, Jamal-Eddine Bouameur, Janina Bär, Robert H Rice, Hue-Tran Hornig-Do, Dennis R Roop, Nicole Schwarz, Susanne Brodesser, Sören Thiering, Rudolf E Leube, Rudolf J Wiesner, Preethi Vijayaraj, Christina B Brazel, Sandra Heller, Hans Binder, Henry Löffler-Wirth, Peter Seibel, Thomas M Magin
Keratin intermediate filaments (KIFs) protect the epidermis against mechanical force, support strong adhesion, help barrier formation, and regulate growth. The mechanisms by which type I and II keratins contribute to these functions remain incompletely understood. Here, we report that mice lacking all type I or type II keratins display severe barrier defects and fragile skin, leading to perinatal mortality with full penetrance. Comparative proteomics of cornified envelopes (CEs) from prenatal KtyI(-/-) and KtyII(-/-)(K8) mice demonstrates that absence of KIF causes dysregulation of many CE constituents, including downregulation of desmoglein 1...
December 7, 2015: Journal of Cell Biology
Songyu Chen, Mingzhi Han, Weiliang Chen, Ying He, Bin Huang, Peng Zhao, Qibing Huang, Liang Gao, Xun Qu, Xingang Li
Malignant glioma is notorious for its aggressiveness and poor prognosis, and the invasiveness of glioma cells is the major obstacle. Accumulating evidence indicates that kinesin superfamily proteins (KIFs) may play key roles in tumor invasiveness, but the mechanisms remained unresolved. Our previous study demonstrated that membrane type 1-matrix metalloproteinase (MT1-MMP) was involved in Kinesin family member 1B (KIF1B)-modulated invasion of gastric cancer cells. Therefore, the role of KIF1B in glioma cell invasion and its relationship with MT1-MMP were explored in the present study...
February 2016: Oncology Reports
Li Jiang, Beatrice M Tam, Guoxing Ying, Sen Wu, William W Hauswirth, Jeanne M Frederick, Orson L Moritz, Wolfgang Baehr
In Caenorhabditis elegans, homodimeric [kinesin family (KIF) 17, osmotic avoidance abnormal-3 (OSM-3)] and heterotrimeric (KIF3) kinesin-2 motors are required to establish sensory cilia by intraflagellar transport (IFT) where KIF3 and KIF17 cooperate to build the axoneme core and KIF17 builds the distal segments. However, the function of KIF17 in vertebrates is unresolved. We expressed full-length and motorless KIF17 constructs in mouse rod photoreceptors using adeno-associated virus in Xenopus laevis rod photoreceptors using a transgene and in ciliated IMCD3 cells...
December 2015: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Na Wang, Junyu Xu
Synaptic plasticity is widely regarded as the cellular basis of learning and memory. Understanding the molecular mechanism of synaptic plasticity has been one of center pieces of neuroscience research for more than three decades. It has been well known that the trafficking of α-amino-3-hydroxy-5-methylisoxazoloe-4-propionic acid- (AMPA-) type, N-methyl-D-aspartate- (NMDA-) type glutamate receptors to and from synapses is a key molecular event underlying many forms of synaptic plasticity. Kainate receptors are another type of glutamate receptors playing important roles in synaptic transmission...
2015: BioMed Research International
Hormos Salimi Dafsari, Susan Byrne, Jean-Pierre Lin, Matthew Pitt, Jan Dh Jongbloed, Frances Flinter, Heinz Jungbluth
Goldberg-Shprintzen megacolon syndrome (GOSHS) (OMIM 609460) is characterized by a combination of learning difficulties, characteristic dysmorphic features and Hirschsprung's disease. Variable clinical features include iris coloboma, congenital heart defects and central nervous system abnormalities, in particular polymicrogyria. GOSHS has been attributed to recessive mutations in KIAA1279, encoding kinesin family member (KIF)-binding protein (KBP) with a crucial role in neuronal microtubule dynamics. Here we report on a 7-year-old girl with GOSHS as a result of a homozygous deletion of exons 5 and 6 of the KIAA1279 gene...
June 2015: American Journal of Medical Genetics. Part A
Nobutaka Hirokawa, Yosuke Tanaka
Kinesin superfamily proteins (KIFs) largely serve as molecular motors on the microtubule system and transport various cellular proteins, macromolecules, and organelles. These transports are fundamental to cellular logistics, and at times, they directly modulate signal transduction by altering the semantics of informational molecules. In this review, we will summarize recent approaches to the regulation of the transport destinations and to the physiological relevance of the role of these proteins in neuroscience, ciliary functions, and metabolic diseases...
May 15, 2015: Experimental Cell Research
Juliana Redondo, Kelly Hares, Alastair Wilkins, Neil Scolding, Kevin Kemp
Axonal injury is often characterized by axonal transport defects and abnormal accumulation of intra-axonal components. Nitric oxide (NO) has a key role in mediating inflammatory axonopathy in many neurodegenerative diseases, but little is known about how nitrosative/oxidative stress affects axonal transport or whether reductions in kinesin superfamily protein (KIF) expression correlate with axon pathology. KIFs are molecular motors that have a key role in axonal and dendritic transport, and impairment of these mechanisms has been associated with a number of neurological disorders...
June 2015: Journal of Neuroscience Research
Viola Tamási, Peter Petschner, Csaba Adori, Eszter Kirilly, Romeo D Ando, Laszlo Tothfalusi, Gabriella Juhasz, Gyorgy Bagdy
OBJECTIVES: Venlafaxine (VLX), a serotonine-noradrenaline reuptake inhibitor, is one of the most commonly used antidepressant drugs in clinical practice for the treatment of major depressive disorder (MDD). Despite being more potent than its predecessors, similarly to them, the therapeutical effect of VLX is visible only 3-4 weeks after the beginning of treatment. Furthermore, recent papers show that antidepressants, including also VLX, enhance the motor recovery after stroke even in non depressed persons...
2014: PloS One
Philip D Campbell, Kimberle Shen, Matthew R Sapio, Thomas D Glenn, William S Talbot, Florence L Marlow
Mutations in Kinesin proteins (Kifs) are linked to various neurological diseases, but the specific and redundant functions of the vertebrate Kifs are incompletely understood. For example, Kif5A, but not other Kinesin-1 heavy-chain family members, is implicated in Charcot-Marie-Tooth disease (CMT) and Hereditary Spastic Paraplegia (HSP), but the mechanism of its involvement in the progressive axonal degeneration characteristic of these diseases is not well understood. We report that zebrafish kif5Aa mutants exhibit hyperexcitability, peripheral polyneuropathy, and axonal degeneration reminiscent of CMT and HSP...
October 29, 2014: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Xin-An Liu, Beena Kadakkuzha, Bruce Pascal, Caitlin Steckler, Komolitdin Akhmedov, Long Yan, Michael Chalmers, Sathyanarayanan V Puthanveettil
Little is known regarding the identity of the population of proteins that are transported and localized to synapses. Here we describe a new approach that involves the isolation and systematic proteomic characterization of molecular motor kinesins to identify the populations of proteins transported to synapses. We used this approach to identify and compare proteins transported to synapses by kinesin (Kif) complexes Kif5C and Kif3A in the mouse hippocampus and prefrontal cortex. Approximately 40-50% of the protein cargos identified in our proteomics analysis of kinesin complexes are known synaptic proteins...
November 11, 2014: Proceedings of the National Academy of Sciences of the United States of America
Shinsuke Niwa
Kinesin superfamily proteins (KIFs) are microtubule-dependent molecular motors that serve as sources of force for intracellular transport and cell division. Recent studies have revealed new roles of KIFs as microtubule stabilizers and depolymerizers, and these activities are fundamental to cellular morphogenesis and mammalian development. KIF2A and KIF19A have microtubule-depolymerizing activities and regulate the neuronal morphology and cilia length, respectively. KIF21A and KIF26A work as microtubule stabilizers that regulate axonal morphology...
January 2015: Anatomical Science International
Despina Sapountzi-Krepia, Maria Psychogiou, Evanthia Sakellari, Maria Tsiligiri, Katri Vehvilainen-Julkunen
This study aimed at exploring Greek fathers' experiences from their wives/partners' labour and delivery, and their perceptions about aspects of delivery care that need to be improved. It took place at hospital maternity clinics at the greater area of Thessaloniki, Greece. Participants were fathers whose wife/partner had given birth one week to one year before the data collection. The data were collected with the use of the Kuopio Instrument for Fathers (KIF). In this article we present the data obtained by KIF's four open-ended questions, which were analyzed using the method of content analysis...
October 2015: International Journal of Nursing Practice
Dorthe Labonté, Edda Thies, Matthias Kneussel
KIF21B, a kinesin family (KIF) protein, is a plus end-directed microtubule motor. The KIF21B gene is highly expressed in neuronal tissue and spleen and is a susceptibility locus for multiple sclerosis. KIF21B motility is regulated through TRIM3, a member of the cytoskeleton-associated-recycling or transport (CART) complex, involved in vesicular receptor recycling. Here we show that the GABAA receptor γ2-subunit co-precipitates and co-localizes with KIF21B in cultured hippocampal neurons. Knockdown of KIF21B gene expression through small hairpin (sh) RNA reduces the number of γ2-subunit-containing GABAA receptor (GABAARs) clusters in neurites and at the cell surface...
August 2014: European Journal of Cell Biology
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