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stability fibrinogen concentrate

N Schäfer, A Driessen, U Bauerfeind, M Fröhlich, J Ofir, E K Stürmer, M Maegele
OBJECTIVES: To analyse which fibrinogen source may improve coagulation using an in vitro 33% dilutional coagulopathy model. BACKGROUND: Uncritical volume resuscitation in the context of trauma haemorrhage contributes to the iatrogenic arm of the acute trauma-induced coagulopathy through dilution and depletion of coagulation factors, with fibrinogen reaching critical levels first. MATERIALS AND METHODS: By using an experimental model of 33% dilutional coagulopathy, we have analysed which fibrinogen source may exert superior effects on improving haemocoagulative capacities and correcting depleted fibrinogen levels...
August 9, 2016: Transfusion Medicine
Christian Zentai, Paola E J van der Meijden, Till Braunschweig, Nicolai Hueck, Markus Honickel, Henri M H Spronk, Rolf Rossaint, Oliver Grottke
BACKGROUND: The potential clinical benefits of targeted therapy with coagulation factor concentrates (e.g., fibrinogen) and antifibrinolytic agents (e.g., tranexamic acid [TXA]) for the treatment of trauma-induced coagulopathy are increasingly recognized. We hypothesized that human fibrinogen concentrate (FC) and prothrombin complex concentrate (PCC), administered as combined therapy with TXA, would provide additive effects for reducing blood loss in an animal trauma model. METHODS: Thirty-six pigs were subjected to 2 consecutive blunt liver injuries, resulting in severe hemorrhagic shock and coagulopathy...
July 2016: Anesthesia and Analgesia
Yan-Bing Wang, Ke-Hui Shi, Hong-Li Jiang, Yong-Kuan Gong
UNLABELLED: A crosslinkable zwitterionic copolymer PMBT was coated onto the surfaces of polypropylene hollow fiber membrane (PP-HFM) oxygenator and its connecting tubes. The PMBT copolymer coating on the oxygenator circuit formed a cell outer membrane mimetic surface with excellent stability. The hemocompatibility of the PMBT copolymer coated PP-HFM oxygenator circuit was evaluated by animal extracorporeal circulation. The concentrations of clotting components fibrinogen and platelet in the blood were almost unchanged during the circulation through the PMBT copolymer coated oxygenator circuits...
August 2016: Acta Biomaterialia
Huiyan Wu, Guangyi Zhao, Hongfei Zu, James H-C Wang, Qing-Ming Wang
In this study, quartz thickness-shear mode (TSM) resonator sensors were adopted to monitor the process of platelet activation. Resting platelets adhering to fibrinogen-coated electrodes were activated by different concentrations of thrombin (1, 10, and 100 U/mL), and the corresponding electrical admittance spectra of TSM resonators during this process were recorded. Based on a bilayer-loading transmission line model of TSM resonators, the complex shear modulus (G' + jG″) and the average thickness (hPL) of the platelet monolayer at a series of time points were obtained...
February 2, 2016: Biophysical Journal
Joerg Nowotny, Jana Farack, Corina Vater, Matthias Johnsen, Michael Gelinsky, Torsten Tonn, Philip Kasten
PURPOSE: Tissue regeneration can be improved by local application of autologous bone marrow derived progenitor cells (BMSC) and platelet rich plasma (PRP). However, there is a lack of standardized application procedures for clinical use. Therefore, a technique in accordance with the guidelines for advanced therapies medical products of the European Medicine Agency was developed and established. METHODS: In detail, a process for the isolation and formulation of autologous bone marrow cells (BMC) and PRP in a clinical setting was validated...
January 2016: Journal of Applied Biomaterials & Functional Materials
Maria Szymonowicz, Zbigniew Rybak, Aneta Fraczek-Szczypta, Danuta Paluch, Agnieszka Rusak, Katarzyna Nowicka, Marta Blazewicz
PURPOSE: Preventing the formation of blood clots on the surface of biomaterials and investigation of the reasons of their formation are the leading topics of the research and development of biomaterials for implants placed into the bloodstream. Biocompatibility and stability of a material in body fluids and direct effect on blood cell counts components are related both to the structure and physico-chemical state of an implant surface. The aim of this study was to determine haemocompatibility and cytotoxicity of polysulfone-based samples containing nano and micro particles of magnetite (Fe3O4)...
2015: Acta of Bioengineering and Biomechanics
Dominika Staniec, Miroslaw Ksiazek, Ida B Thøgersen, Jan J Enghild, Aneta Sroka, Danuta Bryzek, Matthew Bogyo, Magnus Abrahamson, Jan Potempa
Porphyromonas gingivalis is a peptide-fermenting asaccharolytic periodontal pathogen. Its genome contains several genes encoding cysteine peptidases other than gingipains. One of these genes (PG1055) encodes a protein called Tpr (thiol protease) that has sequence similarity to cysteine peptidases of the papain and calpain families. In this study we biochemically characterize Tpr. We found that the 55-kDa Tpr inactive zymogen proteolytically processes itself into active forms of 48, 37, and 33 kDa via sequential truncations at the N terminus...
November 6, 2015: Journal of Biological Chemistry
James R Byrnes, Cédric Duval, Yiming Wang, Caroline E Hansen, Byungwook Ahn, Micah J Mooberry, Martha A Clark, Jill M Johnsen, Susan T Lord, Wilbur A Lam, Joost C M Meijers, Heyu Ni, Robert A S Ariëns, Alisa S Wolberg
Factor XIII(a) [FXIII(a)] stabilizes clots and increases resistance to fibrinolysis and mechanical disruption. FXIIIa also mediates red blood cell (RBC) retention in contracting clots and determines venous thrombus size, suggesting FXIII(a) is a potential target for reducing thrombosis. However, the mechanism by which FXIIIa retains RBCs in clots is unknown. We determined the effect of FXIII(a) on human and murine clot weight and composition. Real-time microscopy revealed extensive RBC loss from clots formed in the absence of FXIIIa activity, and RBCs exhibited transient deformation as they exited the clots...
October 15, 2015: Blood
Vinod Kumar Goyal, Somesh Kakade, Santosh Kumar Pandey, Anil Kalidas Gothi, Ramakrishna Nirogi
Coagulation parameters are usually included in clinical and preclinical safety studies to evaluate the effect of xenobiotics on the extrinsic or intrinsic pathways of coagulation. The analysis is generally performed at the time of terminal sacrifice where many activities are scheduled. Chances of delay in analysis are likely particularly when blood is collected for coagulation via the abdominal vena cava. This experiment was planned to assess the variations in coagulation parameters caused by delay in analysis as well as by storage conditions...
October 2015: Laboratory Animals
W P Sheffield, V Bhakta, C Jenkins
BACKGROUND AND OBJECTIVE: Cryoprecipitate is a concentrated source of fibrinogen and other plasma proteins. Cryoprecipitate must be transfused within 4-6 h of thawing and storage at 20-24°C. We compared plasma protein activities in single or pooled cryoprecipitate units stored at 20-24°C for 0, 4 or 24 h. MATERIALS AND METHODS: Individual cryoprecipitate units (n = 36) were thawed, diluted with sterile saline and sampled over time. Cryoprecipitate pools of eight individual units were assembled either by serial passage of diluent (Method A, n = 6 pools) or by separate dilution into a single collection bag (Method B, n = 6 pools)...
January 2016: Vox Sanguinis
Gonçalo da Costa, Cristina Ribeiro-Silva, Raquel Ribeiro, Samuel Gilberto, Ricardo A Gomes, António Ferreira, Élia Mateus, Eduardo Barroso, Ana V Coelho, Ana Ponces Freire, Carlos Cordeiro
Transthyretin amyloidosis is a conformational pathology characterized by the extracellular formation of amyloid deposits and the progressive impairment of the peripheral nervous system. Point mutations in this tetrameric plasma protein decrease its stability and are linked to disease onset and progression. Since non-mutated transthyretin also forms amyloid in systemic senile amyloidosis and some mutation bearers are asymptomatic throughout their lives, non-genetic factors must also be involved in transthyretin amyloidosis...
2015: PloS One
Joel S Bennett
Blood platelets prevent bleeding after trauma by forming occlusive aggregates at sites of vascular injury. Platelet aggregation is mediated by the integrin heterodimer αIIbβ3 and occurs when platelet agonists generated at the injury site convert αIIbβ3 from its resting to its active conformation. Active αIIbβ3 is then able to bind macromolecular ligands such as fibrinogen that crosslink adjacent platelets into hemostatic aggregates. Platelets circulate in a plasma milieu containing high concentrations of the principal αIIbβ3 ligand fibrinogen...
July 2015: Biopolymers
Buxin Chen, Antonio G Soto, Luisa J Coronel, Ashley Goss, Joanne van Ryn, JoAnn Trejo
Thrombin, the key effector protease of the coagulation cascade, drives fibrin deposition and activates human platelets through protease-activated receptor-1 (PAR1). These processes are critical to the progression of thrombotic diseases. Thrombin is the main target of anticoagulant therapy, and major efforts have led to the discovery of new oral direct inhibitors of thrombin. Dabigatran is the first oral anticoagulant licensed for the prevention of thromboembolisms associated with orthopedic surgery and stroke prevention in atrial fibrillation...
July 2015: Molecular Pharmacology
Medora B Pashmakova, James W Barr, Micah A Bishop
OBJECTIVE: To compare stability of hemostatic proteins in canine fresh-frozen plasma (FFP) thawed with a modified commercial microwave warmer (MCM) or warm water bath (37°C; WWB) or at room temperature (22°C). SAMPLE: Fresh-frozen plasma obtained from 8 canine donors of a commercial blood bank. PROCEDURES: A commercial microwave warmer was modified with a thermocouple to measure surface temperature of bags containing plasma. The MCM and a WWB were each used to concurrently thaw a 60-mL bag of plasma obtained from the same donor...
May 2015: American Journal of Veterinary Research
Jaruwan Siritapetawee, Punchapat Sojikul, Sompong Klaynongsruang
A dimeric protease designated as EuP-82 was purified from Euphorbia cf. lactea latex. Since its proteolytic activity was inhibited by a serine protease specific inhibitor (PMSF), EuP-82 was classified as a serine protease. N-glycan deglycosylation tests revealed that EuP-82 was a glycosylated protein. MALDI-TOF MS showed that EuP-82 was a homodimer, which was its active form. The optimal conditions for fibrinogenolytic activity were at pH 11 and 35 °C. EuP-82 enzyme had broad range of pH stability from 4 to 12...
July 2015: Plant Physiology and Biochemistry: PPB
R B Aĭsina, L I Mukhametova, D V Tiupa, K B Gershkovich, D A Gulin, S D Varfolomeev
By variation of incubation time of streptokinase (SK) with activated polyethylene glycol (M 2 and 5 kDa, PEG2 and PEG5) it was obtained covalent SK-PEG2 and SK-PEG5 conjugates with different modification degrees of amino groups of protein and their properties were studied in vitro as compared with free SK. It was shown, that maximal stable and retaining 80% fibrinolytic activity SK-PEG2 and SK-PEG5 conjugates are formed when the modification degrees of amino groups of protein are 54 and 52%, respectively. At interaction of the given conjugates with equimolar plasminogen concentration it were formed the plasmin (Pm)·SK-PEG2 and Pm·SK-PEG5 activator complexes, the maximal amidase activity of which is equal to activity of unmodified Pm·SK complex...
September 2014: Bioorganicheskaia Khimiia
Seung Hyo Jung, Kyung-Jong Won, Kang Pa Lee, Hyun-Joong Kim, Eun-Hye Seo, Hwan Myung Lee, Eun Seok Park, Seung Hyun Lee, Bokyung Kim
The rupture of an atherosclerotic plaque is one of the main causes of coronary artery thrombotic occlusion, leading to myocardial infarction. However, the exact mechanism and causal risk factors for plaque rupture remain unclear. To identify a potential molecule that can influence atherosclerotic plaque rupture, we investigated protein expression in serum from patients with acute myocardial infarction (AMI) and stable angina (SA), using proteomic analysis. The expression of six proteins, including fibrinogen, fetuin-B, keratin 9, proapolipoprotein and fibrinogen, were altered in serum from patients with AMI compared with serum from those with SA...
July 2015: Clinical Science (1979-)
Laura R Geuss, Alicia C B Allen, Divya Ramamoorthy, Laura J Suggs
Successful cellular cardiomyoplasty is dependent on biocompatible materials that can retain the cells in the myocardium in order to promote host tissue repair following myocardial infarction. A variety of methods have been explored for incorporating a cell-seeded matrix into the heart, the most popular options being direct application of an injectable system or surgical implantation of a patch. Fibrin-based gels are suitable for either of these approaches, as they are biocompatible and have mechanical properties that can be tailored by adjusting the initial fibrinogen concentration...
July 2015: Biotechnology and Bioengineering
Yan Teck Ho, Barbara Poinard, Eugenia Li Ling Yeo, James Chen Yong Kah
Commercial protein assays used ubiquitously in laboratories typically require long incubation times due to the inherently slow protein-reagent reactions. In this study, we report a novel facile technique for the instantaneous measurement of total protein concentration by exploiting the rapid aggregation dynamics of gold nanoparticles (NPs). By adsorbing different amounts of proteins on their surface to form a protein corona, these NPs can be sterically stabilized to different degrees by aggregation, thus exhibiting a spectrum of color change which can be quantitatively characterized by UV-Vis absorption spectroscopy...
February 21, 2015: Analyst
Quanbin Zhang, Guanghua Shan, Ping Cao, Jia He, Zhongshi Lin, Yaoxiong Huang, Ningjian Ao
The goal of this study was to investigate the mechanical and biological properties of oxidized keratin materials, which were obtained by using buffalo horns to oxidize. It could provide a way to evaluate their potential for clinical translatability. The characterization on their composition, mechanical properties, and biological responses was performed. It is found that the oxidation process could lead the disulfide bond to break down and then to form sulfonic acid, or even make partial peptide chain to be fragment for the new modification of amino acid...
February 2015: Materials Science & Engineering. C, Materials for Biological Applications
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