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https://www.readbyqxmd.com/read/27870110/the-hematology-of-captive-bobtail-lizards-tiliqua-rugosa-blood-counts-light-microscopy-cytochemistry-and-ultrastructure
#1
Cheryl A Moller, Tibor Gaál, Jennifer N Mills
INTRODUCTION: Bobtail lizards (Tiliqua rugosa) are native to Australia. The only previous study on the hematology of this species documented just 6 animals. OBJECTIVES: The aims of this study were to characterize the light microscopy, ultrastructure and cytochemistry of blood cells, and evaluate CBCs of captive Bobtail lizards. METHODS: Over 2 consecutive summers, heparinized venous blood was collected from the ventral coccygeal vein of 46 clinically healthy, captive indoor- or outdoor-housed adult Bobtails...
November 21, 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27867890/bone-turnover-markers-emerging-tool-in-the-management-of-osteoporosis
#2
REVIEW
Sahana Shetty, Nitin Kapoor, Joseph Dian Bondu, Nihal Thomas, Thomas Vizhalil Paul
Bone is a dynamic tissue which undergoes constant remodeling throughout the life span. Bone turnover is balanced with coupling of bone formation and resorption at various rates leading to continuous remodeling of bone. A study of bone turnover markers (BTMs) provides an insight of the dynamics of bone turnover in many metabolic bone disorders. An increase in bone turnover seen with aging and pathological states such as osteoporosis leads to deterioration of bone microarchitecture and thus contributes to an increase in the risk of fracture independent of low bone mineral density (BMD)...
November 2016: Indian Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27844089/-biomarkers-for-dementia-and-other-neurodegenerative-diseases-current-developments
#3
J Wiltfang, P Lewczuk, M Otto
Cerebrospinal fluid-based neurochemical dementia diagnostics (CSF-NDD) support the early and differential diagnosis of dementia, most importantly the diagnosis of early or preclinical Alzheimer's dementia (AD). Meanwhile CSF-NDD are now recommended for improved exclusion and positive diagnostics of AD by the German national neuropsychiatry S3 dementia guidelines ( www.DGPPN.de ). Meta-analyses of independent international multicenter studies have shown that a combined CSF analysis of amyloid-beta 1-42 (Aβ 1-42, decreased), total tau proteins (increased) and phospho-tau proteins (increased) offers a sensitivity and specificity of 80-90 % for the early and differential diagnosis of AD (AD versus all other)...
November 14, 2016: Der Nervenarzt
https://www.readbyqxmd.com/read/27812311/abnormal-gel-flotation-caused-by-contrast-media-during-adrenal-vein-sampling
#4
Gabriel Lima-Oliveira, Giuseppe Lippi, Gian Luca Salvagno, Matteo Gelati, Antonella Bassi, Alberto Contro, Francesca Pizzolo, Gian Cesare Guidi
INTRODUCTION: During adrenal venous sampling (AVS) procedure, radiologists administer a contrast agent via the catheter to visualize the proper catheter position. MATERIALS AND METHODS: A patient with primary aldosteronism diagnostic-hypothesis was admitted for AVS. A venogram was performed to
confirm the catheter's position with 2mL of Iopamidol 300 mg/mL. Samples were collected with syringe connected to a hydrophilic coated catheter by low-pressure aspiration from each of the four collection sites: inferior vena cava in the suprarenal portion, inferior vena cava in the infrarenal portion, left adrenal vein, and right adrenal vein; then immediately transferred from syringe to tubes with gel separator...
October 15, 2016: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/27812307/laboratory-testing-of-extravascular-body-fluids-in-croatia-a-survey-of-the-working-group-for-extravascular-body-fluids-of-the-croatian-society-of-medical-biochemistry-and-laboratory-medicine
#5
Lara Milevoj Kopcinovic, Zeljka Vogrinc, Irena Kocijan, Jelena Culej, Merica Aralica, Anja Jokic, Dragana Antoncic, Marija Bozovic
INTRODUCTION: We hypothesized that extravascular body fluid (EBF) analysis in Croatia is not harmonized and aimed to investigate preanalytical, analytical and postanalytical procedures used in EBF analysis in order to identify key aspects that should be addressed in future harmonization attempts. MATERIALS AND METHODS: An anonymous online survey created to explore laboratory testing of EBF was sent to secondary, tertiary and private health care Medical Biochemistry Laboratories (MBLs) in Croatia...
October 15, 2016: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/27812302/continuous-quality-control-of-the-blood-sampling-procedure-using-a-structured-observation-scheme
#6
Tine Lindberg Seemann, Mads Nybo
INTRODUCTION: An observational study was conducted using a structured observation scheme to assess compliance with the local phlebotomy guideline, to identify necessary focus items, and to investigate whether adherence to the phlebotomy guideline improved. MATERIALS AND METHODS: The questionnaire from the EFLM Working Group for the Preanalytical Phase was adapted to local procedures. A pilot study of three months duration was conducted. Based on this, corrective actions were implemented and a follow-up study was conducted...
October 15, 2016: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/27812301/blood-gas-testing-and-related-measurements-national-recommendations-on-behalf-of-the-croatian-society-of-medical-biochemistry-and-laboratory-medicine
#7
REVIEW
Lora Dukić, Lara Milevoj Kopčinović, Adrijana Dorotić, Ivana Baršić
Blood gas analysis (BGA) is exposed to risks of errors caused by improper sampling, transport and storage conditions. The Clinical and Laboratory Standards Institute (CLSI) generated documents with recommendations for avoidance of potential errors caused by sample mishandling. Two main documents related to BGA issued by the CLSI are GP43-A4 (former H11-A4) Procedures for the collection of arterial blood specimens; approved standard - fourth edition, and C46-A2 Blood gas and pH analysis and related measurements; approved guideline - second edition...
October 15, 2016: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/27803290/deciphering-sources-of-variability-in-clinical-pathology-it-s-not-just-about-the-numbers
#8
Niraj K Tripathi, Nancy E Everds, A Eric Schultze, Armando R Irizarry, Robert L Hall, Anne Provencher, Adam Aulbach
The objectives of this session were to explore causes of variability in clinical pathology data due to preanalytical and analytical variables as well as study design and other procedures that occur in toxicity testing studies. The presenters highlighted challenges associated with such variability in differentiating test article-related effects from the effects of experimental procedures and its impact on overall data interpretation. These presentations focused on preanalytical and analytical variables and study design-related factors and their influence on clinical pathology data, and the importance of various factors that influence data interpretation including statistical analysis and reference intervals...
November 1, 2016: Toxicologic Pathology
https://www.readbyqxmd.com/read/27784640/preanalytics-in-urinalysis
#9
REVIEW
Joris R Delanghe, Marijn M Speeckaert
Urine contains an enormous amount of information. Well-standardized procedures for collection, transport, sample preparation and analysis should become the basis of an effective diagnostic strategy for urinalysis. As reproducibility of urinalysis has been greatly improved due to recent technological progress, preanalytical requirements of urinalysis have gained importance and have become stricter. Since the patients themselves often collect urine specimens, urinalysis is very susceptible to preanalytical issues...
December 2016: Clinical Biochemistry
https://www.readbyqxmd.com/read/27777903/exosomes-as-the-source-of-biomarkers-of-metabolic-diseases
#10
Min-Jae Lee, Dong-Ho Park, Ju-Hee Kang
Exosomes are extracellular vesicles that contain molecules that regulate the metabolic functions of adjacent or remote cells. Recent in vitro, in vivo and clinical studies support the hypothesis that exosomes released from various cell types play roles in the progression of metabolic disorders including type 2 diabetes. Based on this concept and advances in other diseases, the proteins, mRNA, microRNA and lipids in exosomes isolated from biological fluids have been proposed as biomarkers in metabolic disorders...
September 2016: Annals of Pediatric Endocrinology & Metabolism
https://www.readbyqxmd.com/read/27771343/re-engineering-laboratory-diagnostics-for-preventing-preanalytical-errors
#11
EDITORIAL
Giuseppe Lippi, Raffick Bowen, Dorothy M Adcock
No abstract text is available yet for this article.
December 2016: Clinical Biochemistry
https://www.readbyqxmd.com/read/27765677/blood-collection-tubes-as-medical-devices-the-potential-to-affect-assays-and-proposed-verification-and-validation-processes-for-the-clinical-laboratory
#12
REVIEW
Raffick A R Bowen, Dorothy M Adcock
Blood collection tubes (BCTs) are an often under-recognized variable in the preanalytical phase of clinical laboratory testing. Unfortunately, even the best-designed and manufactured BCTs may not work well in all clinical settings. Clinical laboratories, in collaboration with healthcare providers, should carefully evaluate BCTs prior to putting them into clinical use to determine their limitations and ensure that patients are not placed at risk because of inaccuracies due to poor tube performance. Selection of the best BCTs can be achieved through comparing advertising materials, reviewing the literature, observing the device at a scientific meeting, receiving a demonstration, evaluating the device under simulated conditions, or testing the device with patient samples...
December 2016: Clinical Biochemistry
https://www.readbyqxmd.com/read/27753037/methodological-variables-in-the-analysis-of-cell-free-dna
#13
Abel Jacobus Bronkhorst, Janine Aucamp, Piet J Pretorius
In recent years, cell-free DNA (cfDNA) analysis has received increasing amounts of attention as a potential non-invasive screening tool for the early detection of genetic aberrations and a wide variety of diseases, especially cancer. However, except for some prenatal tests and BEAMing, a technique used to detect mutations in various genes of cancer patients, cfDNA analysis is not yet routinely applied in clinical practice. Although some confusing biological factors inherent to the in vivo setting play a key part, it is becoming increasingly clear that this struggle is mainly due to the lack of an analytical consensus, especially as regards quantitative analyses of cfDNA...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27735096/preanalytical-stability-of-adrenocorticotropic-hormone-depends-on-both-time-to-centrifugation-and-temperature
#14
Zhi-Qi Wu, Hua-Guo Xu
OBJECTIVE: The purpose of our study was to analyze the effects of temperature, time delay, and time to centrifugation on the stability of human plasma adrenocorticotropin (ACTH) measurements. METHODS: Twenty-one EDTA whole blood sample pools were centrifuged at 1100 ×g for 10 minutes at 4°C either immediately or after storage for 2, 4, 8, and 24 hours at 4°C or room temperature. Plasma ACTH was then measured either immediately or after 2, 4, 8, and 24 hours storage at 4°C or room temperature...
October 13, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27720280/preanalytical-considerations-for-joint-fluid-evaluation
#15
REVIEW
Caitlyn R Martinez, Kelly S Santangelo
Synovial fluid analysis is a key component of the minimum database needed to diagnose and manage primary and secondary articular joint disorders. Unfortunately, preanalytical variables can drastically alter samples submitted for evaluation to veterinary laboratories and it is considered the stage at which most laboratory error occurs. This article addresses common sources of preanalytical variability and error that are seen in veterinary medicine. With consistent quality control and reporting of specimens, downstream clinical decision making and management of patients can be accelerated and improved...
January 2017: Veterinary Clinics of North America. Small Animal Practice
https://www.readbyqxmd.com/read/27717415/monitoring-oxygen-status
#16
J G Toffaletti, C R Rackley
Although part of a common "blood gas" test panel with pH and pCO2, the pO2, %O2Hb, and related parameters are independently used to detect and monitor oxygen deficits from a variety of causes. Measurement of blood gases and cooximetry may be done by laboratory analyzers, point of care testing, noninvasive pulse oximetry, and transcutaneous blood gases. The specimen type and mode of monitoring oxygenation that are chosen may be based on a combination of urgency, practicality, clinical need, and therapeutic objectives...
2016: Advances in Clinical Chemistry
https://www.readbyqxmd.com/read/27717198/young-investigator-challenge-can-the-ion-ampliseq-cancer-hotspot-panel-v2-be-used-for-next-generation-sequencing-of-thyroid-fna-samples
#17
Claudio Bellevicine, Roberta Sgariglia, Umberto Malapelle, Elena Vigliar, Mariantonia Nacchio, Giuseppe Ciancia, Markus Eszlinger, Ralf Paschke, Giancarlo Troncone
BACKGROUND: Fine-needle aspiration (FNA) cytology is accurate and cost-effective in the evaluation of thyroid nodules. Molecular techniques may contribute to risk stratification in indeterminate cases. Although next-generation sequencing (NGS) is a promising technique for the molecular testing of thyroid FNA specimens, thyroid-specific cancer gene panels are not commercially available. Conversely, the Ion AmpliSeq Cancer Hotspot Panel v2 (CHPv2), which includes the genes most frequently mutated in thyroid neoplasms, is commercially available and may represent an alternative to thyroid-specific panels...
November 2016: Cancer
https://www.readbyqxmd.com/read/27686172/significant-reduction-in-preanalytical-errors-for-nonphlebotomy-blood-draws-after-implementation-of-a-novel-integrated-specimen-collection-module
#18
Rachel D Le, Stacy E F Melanson, Athena K Petrides, Ellen M Goonan, Ida Bixho, Adam B Landman, Anne Marie Brogan, David W Bates, Milenko J Tanasijevic
OBJECTIVES: Most preanalytical errors at our institution occur during nonphlebotomy blood draws. We implemented an electronic health record (EHR), interfaced the EHR to the laboratory information system, and designed a new specimen collection module. We studied the effects of the new system on nonphlebotomy preanalytical errors. METHODS: We used an electronic database of preanalytical errors and calculated the number and type of the most common errors in the emergency department (ED) and inpatient nursing for 3-month periods before (August-October 2014) and after (August-October 2015) implementation...
October 2016: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27683525/the-clinical-value-of-soluble-urokinase-plasminogen-activator-receptor-supar-levels-in-autoimmune-connective-tissue-disorders
#19
Barna Vasarhelyi, Gergely Toldi, Attila Balog
The assessment of the general inflammatory condition of patients with autoimmune connective tissue disorders (ACTD) is a major challenge. The use of traditional inflammatory markers including CRP-levels and erythrocyte sedimentation rate (ESR) is limited by several preanalytical factors and their low specificities. Soluble urokinase plasminogen activator receptor (suPAR) is one of the novel candidate markers that is increasingly used in immune mediated disorders. In our studies we compared suPAR levels of patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), systemic sclerosis (SSc) and ankylosing spondylitis with those of healthy controls...
April 2016: EJIFCC
https://www.readbyqxmd.com/read/27672657/18s-ribosomal-rna-evaluation-as-preanalytical-quality-control-for-animal-dna
#20
Cory Ann Leonard, Marina L Meli, Marilisa Novacco, Nicole Borel
The 18S ribosomal RNA (rRNA) gene is present in all eukaryotic cells. In this study, we evaluated the use of this gene to verify the presence of PCR-amplifiable host (animal) DNA as an indicator of sufficient sample quality for quantitative real-time PCR (qPCR) analysis. We compared (i) samples from various animal species, tissues, and sample types, including swabs; (ii) multiple DNA extraction methods; and (iii) both fresh and formalin-fixed paraffin-embedded (FFPE) samples. Results showed that 18S ribosomal RNA gene amplification was possible from all tissue samples evaluated, including avian, reptile, and FFPE samples and most swab samples...
2016: BioMed Research International
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