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https://www.readbyqxmd.com/read/28431643/advances-in-circulating-tumor-dna-analysis
#1
Samantha Perakis, Martina Auer, Jelena Belic, Ellen Heitzer
The analysis of cell-free circulating tumor DNA (ctDNA) is a very promising tool and might revolutionize cancer care with respect to early detection, identification of minimal residual disease, assessment of treatment response, and monitoring tumor evolution. ctDNA analysis, often referred to as "liquid biopsy" offers what tissue biopsies cannot-a continuous monitoring of tumor-specific changes during the entire course of the disease. Owing to technological improvements, efforts for the establishment of preanalytical and analytical benchmark, and the inclusion of ctDNA analyses in clinical trial, an actual clinical implementation has come within easy reach...
2017: Advances in Clinical Chemistry
https://www.readbyqxmd.com/read/28429254/biomarkers-predicting-bone-turnover-in-the-setting-of-ckd
#2
REVIEW
Pieter Evenepoel, Etienne Cavalier, Patrick C D'Haese
PURPOSE OF THE REVIEW: Impaired bone quality contributes to the increased fracture risk in chronic kidney disease patients. Both low and high turnover bone disease may compromise bone quality. The question arises whether bone biomarkers may be additive or replace bone histormorphometry for diagnosing the extremes of bone turnover. RECENT FINDINGS: Studies exploring the performance of established and emerging bone biomarkers against histomorphometric assessment of bone turnover are limited and overall yield inconclusive results as to their diagnostic utility...
April 20, 2017: Current Osteoporosis Reports
https://www.readbyqxmd.com/read/28428356/biological-variation-estimates-obtained-from-91-healthy-study-participants-for-9-enzymes-in-serum
#3
Anna Carobene, Thomas Røraas, Una Ørvim Sølvik, Marit Sverresdotter Sylte, Sverre Sandberg, Elena Guerra, Irene Marino, Niels Jonker, Gerhard Barla, William A Bartlett, Pilar Fernandez-Calle, Jorge Díaz-Garzón, Francesca Tosato, Mario Plebani, Abdurrahman Coŝkun, Mustafa Serteser, Ibrahim Unsal, Ferruccio Ceriotti
BACKGROUND: We sought to develop estimates of biological variation (BV) for 9 enzymes in blood serum as part of the European Biological Variation Study. METHODS: Ninety-one healthy study participants (38 male and 53 female, 21-69 years old) were phlebotomized in each of 10 consecutive weeks at 6 European laboratories. The same preanalytical sample-handling protocol was followed at each center before transport to San Raffaele Hospital, Milan, Italy, for analysis...
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28427918/endocan-is-a-stable-circulating-molecule-in-icu-patients
#4
Gaudet Alexandre, Chenevier-Gobeaux Camille, Parmentier Erika, Delobel Jean-Edouard, Dubucquoi Sylvain, Mathieu Daniel, Lassalle Philippe, De Freitas Caires Nathalie
BACKGROUND: Endocan is a lung endothelial cell secreted proteoglycan, possessing multiple physiological roles and potential therapeutic and diagnostic utility as biomarker in pneumonia and acute respiratory distress syndrome. Endocan synthesis and secretion can be induced by proinflammatory cytokines such as TNF-α, but can also be subject of proteolytic degradation causing preanalytical variation. METHODS: We investigated the stability of endocan in conventional serum, plasma, anticoagulated whole blood, as well as whole blood and plasma stabilized with protease inhibitors...
April 17, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28421449/flow-cytometric-analysis-of-the-ercc1-marker-of-dna-damage-repair-in-the-tumor-specimens-embedded-into-paraffin-blocks
#5
T A Bogush, E A Dudko, A N Grishanina, V T Zarkua, E A Bogush, A A Basharina, B E Polotsky, S A Tjulandin, M M Davydov, M I Davydov
The differences in expression of ERCC1 were estimated between tumor specimens embedded into paraffin blocks and surgical biopsy specimens of non-small cell lung cancer as well as breast and ovarian cancers. Concordance or differences not higher than 20% were observed in 73% of the cases. The number of the cases with more significant differences in ERCC1 expression was less than 17%. The results show that ERCC1 detection in surgical biopsy specimens by flow cytometry is the more preferable method due to reduced preanalytical phase of the analysis...
January 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28416603/high-throughput-immunogenetics-for-clinical-and-research-applications-in-immunohematology-potential-and-challenges
#6
Anton W Langerak, Monika Brüggemann, Frédéric Davi, Nikos Darzentas, Jacques J M van Dongen, David Gonzalez, Gianni Cazzaniga, Véronique Giudicelli, Marie-Paule Lefranc, Mathieu Giraud, Elizabeth A Macintyre, Michael Hummel, Christiane Pott, Patricia J T A Groenen, Kostas Stamatopoulos
Analysis and interpretation of Ig and TCR gene rearrangements in the conventional, low-throughput way have their limitations in terms of resolution, coverage, and biases. With the advent of high-throughput, next-generation sequencing (NGS) technologies, a deeper analysis of Ig and/or TCR (IG/TR) gene rearrangements is now within reach, which impacts on all main applications of IG/TR immunogenetic analysis. To bridge the generation gap from low- to high-throughput analysis, the EuroClonality-NGS Consortium has been formed, with the main objectives to develop, standardize, and validate the entire workflow of IG/TR NGS assays for 1) clonality assessment, 2) minimal residual disease detection, and 3) repertoire analysis...
April 17, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28412284/effects-of-preanalytical-factors-on-hemoglobin-measurement-a-comparison-of-two-hemocue%C3%A2-point-of-care-analyzers
#7
Ralph D Whitehead, Ming Zhang, Maya R Sternberg, Rosemary L Schleicher, Bakary Drammeh, Carine Mapango, Christine M Pfeiffer
BACKGROUND: In field studies, hemoglobin (Hb) is often measured using a battery-operated, portable HemoCue® hemoglobinometer. METHODS: We compared the performance of 2 HemoCue® models (Hb-201+ and Hb-301) and investigated effects of preanalytical factors on Hb results by simulating unfavorable field conditions. RESULTS: The Hb-301 produced 2.6% higher results compared to the Hb-201+. Hb had to be measured within 1min of filling the Hb-301 cuvette to avoid artificially elevated concentrations (1...
April 12, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28402169/delta-check-practices-and-outcomes-a-q-probes-study-involving-49-health-care-facilities-and-6541-delta-check-alerts
#8
Ron B Schifman, Michael Talbert, Rhona J Souers
CONTEXT: - Delta checks serve as a patient-based quality control tool to detect testing problems. OBJECTIVE: - To evaluate delta check practices and outcomes. DESIGN: - Q-Probes participants provided information about delta check policies and procedures. Information about investigations, problems, and corrective actions was prospectively collected for up to 100 testing episodes involving delta check alerts. RESULTS: - Among 4505 testing episodes involving 6541 delta check alerts, the median frequencies of actions taken among 49 laboratories were clinical review, 38...
April 12, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/28392739/preanalytical-investigations-of-phlebotomy-methodological-aspects-pitfalls-and-recommendations
#9
REVIEW
Cristiano Ialongo, Sergio Bernardini
Phlebotomy is often addressed as a crucial process in the pre-analytical phase, in which a large part of laboratory errors take place, but to date there is not yet a consolidated methodological paradigm. Seeking literature, we found 36 suitable investigations issued between 1996 and 2016 (April) dealing with the investigation of pre-analytical factors related to phlebotomy. We found that the largest part of studies had a cohort of healthy volunteers (22/36) or outpatients (11/36), with the former group showing a significantly smaller median sample size (N = 20, IQR: 17...
February 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/28392736/preanalytical-external-quality-assessment-of-the-croatian-society-of-medical-biochemistry-and-laboratory-medicine-and-croqalm-finding-undetected-weak-spots
#10
Nora Nikolac, Jasna Lenicek Krleza, Ana-Maria Simundic
INTRODUCTION: The aim of this paper is to present results of first two years of preanalytical external quality assessment (EQA) in Croatia. MATERIALS AND METHODS: This paper summarizes results from 6 rounds of preanalytical EQA during 2014-2016 in 161-175 Croatian laboratories (number ranged between cycles). EQA was designed as an online survey of the compliance with National recommendations for phlebotomy (NRP). Forty-seven questions in 5 categories are analyzed (materials and equipment, patient identification, patient preparation, sampling and storage)...
February 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/28377328/preanalytical-stability-of-maternal-serum-markers-hcg%C3%AE-and-papp-a
#11
Béatrice Veyrat, François Tosetti, Jean-François Morin, Marie-Pierre Moineau, Andrée Piedimonte, Patrice Clément, Sophie Dreux, Françoise Muller
Down syndrome maternal serum marker screening is based on a risk calculation including the free β - human chorionic gonadotropin (hCGβ) and pregnancy-associated placenta protein type A (PAPP-A). The aim of this study was to define the pre-analytical conditions of stability of these markers both in whole blood at 15-25 ̊C and, after centrifugation, in serum at 4-8 ̊C. 158 patients were included in the study. Two automated workstations were used for assays, Cobas 8000e602, Roche Diagnostics (58 patients tested) and DELFIAXpress, PerkinElmer (100 patients tested)...
April 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28372954/stability-of-serum-plasma-and-urine-osmolality-in-different-storage-conditions-relevance-of-temperature-and-centrifugation
#12
Macià Sureda-Vives, Daniel Morell-Garcia, Ana Rubio-Alaejos, Laura Valiña, Juan Robles, Josep Miquel Bauça
BACKGROUND: Osmolality reflects the concentration of all dissolved particles in a body fluid, and its measurement is routinely performed in clinical laboratories for the differential diagnosis of disorders related with the hydrolytic balance regulation, the renal function and in small-molecule poisonings. The aim of the study was to assess the stability of serum, plasma and urine osmolality through time and under different common storage conditions, including delayed centrifugation. METHODS: Blood and urine samples were collected, and classified into different groups according to several preanalytical variables: serum or plasma lithium-heparin tubes; spun or unspun; stored at room temperature (RT), at 4°C or frozen at -21°C...
March 31, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28370131/use-of-allogeneic-apheresis-stem-cell-products-as-an-interlaboratory-proficiency-challenge
#13
Laura Cooling, Kelly Roxbury, Sandra Hoffmann, Joan DeBusscher, Usha Kota, Steven Goldstein, Robertson Davenport
BACKGROUND: AABB Standards requires that laboratories participate in a proficiency test (PT) program for critical analytes. Institutions can purchase commercial PT materials; however, PT can also be performed through interlaboratory exchange. We investigated the utility of allogeneic hematopoietic progenitor cell apheresis (HPC-A) products as an interlaboratory PT challenge for total nucleated cell count (TNC) and CD34 assessment. STUDY DESIGN AND METHODS: Three-year retrospective and comparative review of unrelated allogeneic HPC-A products received by the University of Michigan between January 2011 and December 2013...
March 28, 2017: Transfusion
https://www.readbyqxmd.com/read/28370080/evaluation-of-assays-for-quantification-of-dna-in-canine-plasma-as-an-indirect-marker-of-netosis
#14
Stephanie A Smith, Corinne M Lawson, Maureen A McMichael, Katrina Jung, Mauria O'Brien, Ron Achiel
BACKGROUND: Neutrophil extracellular traps (NET), consisting of a filamentous DNA/chromatin-histone scaffold originating from neutrophils are part of the innate immune response, may be released under a variety of inflammatory conditions and are associated with an increased risk for thrombosis. OBJECTIVES: The purpose of this study was to evaluate a SYTOX green fluorescence assay and a histone-DNA complex (hisDNA) ELISA for quantification of NET-related DNA in canine plasma...
April 3, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28356887/pre-test-and-post-test-applications-to-shape-the-education-of-phlebotomists-in-a-quality-management-program-an-experience-in-a-training-hospital
#15
Güzin Aykal, Mustafa Keşapli, Özgür Aydin, Hatice Esen, Ayşenur Yeğin, Faruk Güngör, Necat Yilmaz
BACKGROUND: After the introduction of modern laboratory instruments and information systems, preanalytic phase is the new field of battle. Errors in preanalytical phase account for approximately half of total errors in clinical laboratory. The objective of this study was to share an experience of an education program that was believed to be successful in decreasing the number of rejected samples received from the Emergency Department (ED). METHODS: An education program about laboratory procedures, quality requirements in the laboratory, patient and health-care worker safety was planned by the quality team to be performed on 36 people who were responsible for sample collection in the ED...
September 2016: Journal of Medical Biochemistry
https://www.readbyqxmd.com/read/28347209/stability-of-glomerular-and-tubular-renal-injury-biomarkers-in-canine-urine-after-4-years-of-storage
#16
Pieter Defauw, Evelyne Meyer, Luc Duchateau, Johan P Schoeman, Isabel Van de Maele, Sylvie Daminet
Urine biomarkers are sensitive indicators of early-stage renal injury, consequently, research in this area is expanding in both human and veterinary medicine. However, studies investigating the impact of preanalytical factors, such as storage conditions, on urine biomarker concentrations are largely lacking in veterinary medicine. Therefore, we evaluated the stability of several renal injury biomarkers in canine urine after storage for 4 y at -72°C. Urine samples were collected from 26 dogs: 18 dogs with babesiosis and 8 healthy dogs...
March 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28346702/evaluation-of-the-effects-of-time-temperature-and-specimen-storage-on-in-vitro-lactate-concentrations-in-healthy-dogs
#17
Colin F Reich, Jonathan F Bach, Julie M Walker
BACKGROUND: Lactate concentrations increase significantly under certain storage conditions, except for when glycolysis-arresting agents are used. Evaluation of time and storage conditions on heparinized whole blood lactate concentrations without glycolysis-arresting agents have not been evaluated in dogs. OBJECTIVES: The purpose of the study was to determine the effects of preanalytic storage conditions on the in vitro concentration of lactate in heparinized canine venous blood specimens...
March 27, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28340232/a-proposed-set-of-metrics-to-reduce-patient-safety-risk-from-within-the-anatomic-pathology-laboratory
#18
Peter Banks, Richard Brown, Alex Laslowski, Yvonne Daniels, Phil Branton, John Carpenter, Richard Zarbo, Ramses Forsyth, Yan-Hui Liu, Shane Kohl, Joachim Diebold, Shinobu Masuda, Tim Plummer, Eslie Dennis
Background: Anatomic pathology laboratory workflow consists of 3 major specimen handling processes. Among the workflow are preanalytic, analytic, and postanalytic phases that contain multistep subprocesses with great impact on patient care. A worldwide representation of experts came together to create a system of metrics, as a basis for laboratories worldwide, to help them evaluate and improve specimen handling to reduce patient safety risk. Method: Members of the Initiative for Anatomic Pathology Laboratory Patient Safety (IAPLPS) pooled their extensive expertise to generate a list of metrics highlighting processes with high and low risk for adverse patient outcomes...
March 18, 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/28321418/ana-iif-automation-moving-towards-harmonization-results-of-a-multicenter-study
#19
MULTICENTER STUDY
Stefanie Van den Bremt, Sofie Schouwers, Marjan Van Blerk, Lieve Van Hoovels
Background. Our study aimed to investigate whether the introduction of automated anti-nuclear antibody (ANA) indirect immunofluorescence (IIF) analysis decreases the interlaboratory variability of ANA titer results. Method. Three serum samples were sent to 10 laboratories using the QUANTA-Lyser® in combination with the NOVA View®. Each laboratory performed the ANA IIF analysis 10x in 1 run and 1x in 10 different runs and determined the endpoint titer by dilution. One of the three samples had been sent in 2012, before the era of ANA IIF automation, by the Belgian National External Quality Assessment (EQA) Scheme...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28316435/delta-check-for-blood-groups-a-step-ahead-in-blood-safety
#20
Raj Nath Makroo, Aakanksha Bhatia
BACKGROUND: Blood grouping is the single most important test performed by each and every transfusion service. A blood group error has a potential for causing severe life-threatening complications. A number of process strategies have been adopted at various institutions to prevent the occurrence of errors at the time of phlebotomy, pretransfusion testing, and blood administration. A delta check is one such quality control tool that involves the comparison of laboratory test results with results obtained on previous samples from the same patient...
January 2017: Asian Journal of Transfusion Science
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