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Jamie Toombs, Martha S Foiani, Henrietta Wellington, Ross W Paterson, Charles Arber, Amanda Heslegrave, Michael P Lunn, Jonathan M Schott, Selina Wray, Henrik Zetterberg
Introduction: We tested the hypothesis that the amyloid β (Aβ) peptide ratios are more stable than Aβ42 alone when biofluids are exposed to two preanalytical conditions known to modify measurable Aβ concentration. Methods: Human cerebrospinal fluid (CSF) and culture media (CM) from human cortical neurons were exposed to a series of volumes and polypropylene surfaces. Aβ42 , Aβ40 , and Aβ38 peptide concentrations were measured using a multiplexed electrochemiluminescence immunoassay...
2018: Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring
Daniel R Bauer, Michael Otter, David R Chafin
Purpose of Review: Studying and developing preanalytical tools and technologies for the purpose of obtaining high-quality samples for histological assays is a growing field. Currently, there does not exist a standard practice for collecting, fixing, and monitoring these precious samples. There has been some advancement in standardizing collection for the highest profile tumor types, such as breast, where HER2 testing drives therapeutic decisions. This review examines the area of tissue collection, transport, and monitoring of formalin diffusion and details a prototype system that could be used to help standardize tissue collection efforts...
2018: Current Pathobiology Reports
Robert Brunkhorst, Waltraud Pfeilschifter, Sammy Patyna, Stefan Büttner, Timon Eckes, Sandra Trautmann, Dominique Thomas, Josef Pfeilschifter, Alexander Koch
Dysregulation of blood sphingolipids is an emerging topic in clinical science. The objective of this study was to determine preanalytical biases that typically occur in clinical and translational studies and that influence measured blood sphingolipid levels. Therefore, we collected blood samples from four healthy male volunteers to investigate the effect of storage conditions (time, temperature, long-term storage, freeze⁻thaw cycles), blood drawing (venous or arterial sampling, prolonged venous compression), and sample preparation (centrifugation, freezing) on sphingolipid levels measured by LC-MS/MS...
May 7, 2018: International Journal of Molecular Sciences
Andrea Padoan, Renata D'Incà, Maria Luisa Scapellato, Rudi De Bastiani, Roberta Caccaro, Claudia Mescoli, Stefania Moz, Dania Bozzato, Carlo-Federico Zambon, Greta Lorenzon, Massimo Rugge, Mario Plebani, Daniela Basso
BACKGROUND: The appropriate clinical use of fecal calprotectin (fCal) might be compromised by incomplete harmonization between assays and within- and between-subjects variability. Our aim was to investigate the analytical and biological variability of fCal in order to provide tools for interpreting fCal in the clinical setting. METHODS: Experiments were conducted to investigate the effects of temperature and storage time on fCal. Thirty-nine controls were enrolled to verify biological variability, and a case-control study was conducted on 134 controls and 110 IBD patients to compare the clinical effectiveness of three different fCal assays: ELISA, CLIA and turbidimetry...
May 5, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Renato Tozzoli, Nicola Bizzaro
In this review we analyze all aspects of total testing process regarding the measurement of antithyroid peroxidase, antithyroglobulin and antithyrotropin receptor autoantibodies. The main critical points related to the preanalytical, analytical and postanalytical steps of autoimmune thyroid disease diagnostics are considered, focusing on harmonization of autoimmune thyroid tests request, retesting intervals, terminology of thyroid autoantibodies, measurement units and definition of reference limits. Harmonization in thyroid autoantibody testing is a relevant example of feasible harmonization in autoimmunology...
May 5, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Ruta Gupta, Wendy A Cooper, Christina Selinger, Annabelle Mahar, Lyndal Anderson, Michael E Buckland, Sandra A O'Toole
There have been rapid and significant advances in diagnostic and predictive molecular techniques in recent years with profound impact on patient care. In situ hybridization (ISH) studies have become well entrenched in surgical pathology practice and their role in the evaluation of HER2 in breast carcinoma and their diagnostic utility in soft tissue pathology are well known. Fluorescent ISH is being increasingly used in other sites such as the head and neck and the gynecologic tract. Like most tests in surgical pathology, ISH studies require good quality tissue, correlation with clinical and histopathologic findings, and adherence to guidelines for optimal assay performance and interpretation...
May 3, 2018: Advances in Anatomic Pathology
Sergiu Susman, Ioana Berindan-Neagoe, Bobe Petrushev, Radu Pirlog, Ioan-Stefan Florian, Carmen-Mihaela Mihu, Cristian Berce, Lucian Craciun, Ravnit Grewal, Ciprian Tomuleasa
After introducing the new molecules for the treatment of patients with tumoral pathology, the therapeutical decision will be taken depending on the molecular profile performed upon the harvested tissues. This major modification makes the molecular and morphological analysis an essential part in the clinical management of patients and the pathologist plays an important role in this process. The quality and reproducibility of the results are imperative today and they depend on both the reliability of the molecular techniques and the quality of the tissue we use in the process...
2018: Cancer Management and Research
Pitchaikaran Arul, Magesh Pushparaj, Kanmani Pandian, Lingasamy Chennimalai, Karthika Rajendran, Eniya Selvaraj, Suresh Masilamani
BACKGROUND: An important component of laboratory medicine is preanalytical phase. Since laboratory report plays a major role in patient management, more importance should be given to the quality of laboratory tests. AIM: The present study was undertaken to find the prevalence and types of preanalytical errors at a tertiary care hospital in South India. MATERIALS AND METHODS: In this cross-sectional study, a total of 118,732 samples ([62,474 outpatient department [OPD] and 56,258 inpatient department [IPD]) were received in hematology laboratory...
April 2018: Journal of Laboratory Physicians
Katja Kemp Jacobsen, Ida Brandt, Anne Vindahl Christensen, Bjørk Anine Rimsø, Camilla Julie Krøier, Michelle Sørensen, Julie Smith, Kathrine Overgaard Foss Jensen, Jeppe Madura Larsen
BACKGROUND: Deviation in blood collection procedures is a central source of preanalytical variation affecting overall analytical and diagnostic precision. The order of draw of venous sampling is suspected to affect analytical results, in particular for coagulation analysis. Here we compare the procedures in venous blood sampling among clinical biochemistry departments to assess the uniformity of order of blood draw and adherence to international guidelines in the Danish health care system...
April 20, 2018: Clinical Biochemistry
G Unger, S F Benozzi, A Campion, G L Pennacchiotti
BACKGROUND: We analyze the effects of water ingestion before blood extraction on routine hematological parameters. METHODS: Twenty female volunteers -mean 24 y- were included. Blood was collected after a 12 h fast period (T0 ) and 1 h after the ingestion of 300 ml water (T1 ). These parameters were analyzed: white blood cell (WBC) count; WBC differential count including lymphocytes (lym), monocytes (mono), neutrophils, eosinophils (eos), and basophils; red blood cell (RBC) count; hematocrit (hct); hemoglobin (hgb); mean cell volume; mean cell hemoglobin; RBC distribution width; and platelet count (plt)...
April 17, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Graham R D Jones, Rainer Haeckel, Tze Ping Loh, Ken Sikaris, Thomas Streichert, Alex Katayev, Julian H Barth, Yesim Ozarda
Reference intervals are a vital part of the information supplied by clinical laboratories to support interpretation of numerical pathology results such as are produced in clinical chemistry and hematology laboratories. The traditional method for establishing reference intervals, known as the direct approach, is based on collecting samples from members of a preselected reference population, making the measurements and then determining the intervals. An alternative approach is to perform analysis of results generated as part of routine pathology testing and using appropriate statistical techniques to determine reference intervals...
April 19, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Annalise E Zemlin
The total testing process consists of various phases from the pre-preanalytical to the post-postanalytical phase, the so-called brain-to-brain loop. With improvements in analytical techniques and efficient quality control programmes, most laboratory errors now occur in the extra-analytical phases. There has been recent interest in these errors with numerous publications highlighting their effect on service delivery, patient care and cost. This interest has led to the formation of various working groups whose mission is to develop standardized quality indicators which can be used to measure the performance of service of these phases...
April 2018: Indian Journal of Clinical Biochemistry: IJCB
Frank M Brunkhorst, Petra Gastmeier, Muna Abu Sin
Hospital mortality of patients with secondary sepsis remains high at around 40%. Because of the methodological deficiencies of the definitions used so far, valid epidemiological data on secondary sepsis that allow for national and international comparisons are lacking. Since 2016, new clinical diagnostic tools that are also suitable for sepsis screening outside of intensive care units have been available. To counteract the high mortality of nosocomial sepsis, new approaches to the early identification of at-risk patients are needed...
May 2018: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
Franz Ratzinger, Mona Lang, Sabine Belik, Klaus G Schmetterer, Helmuth Haslacher, Thomas Perkmann, Peter Quehenberger
CONTEXT: - Coagulation testing is challenging and depends on preanalytic factors, including the citrate buffer concentration used. OBJECTIVE: - To better estimate preanalytic effects of the citrate buffer concentration in use, the difference between results obtained by samples with 3.2% and 3.8% citrate was evaluated. DESIGN: - In a prospective observational study with 76 volunteers, differences related to the citrate concentration were evaluated...
April 6, 2018: Archives of Pathology & Laboratory Medicine
K-F Becker, J Wipperfürth, E Herpel
Access to well-characterized human biosamples is one of the most important prerequisites for modern biomedical research. Biobanks play a decisive role here, as they provide corresponding biosamples for planned analyses. Many interfering factors influencing the quality of biosamples have to be taken into account. In addition to logistical, ethical, and data protection aspects, preanalytical variables in the context of sample acquisition, storage, and processing should be mentioned in particular. In this paper, therefore, the most important preanalytical influencing factors are presented systematically and an overview of current national and international activities for the standardized recording of these factors is provided with the goal of being able to better understand their influence on results and to minimize them in the near future...
April 4, 2018: Der Pathologe
Alexander von Meyer, Janne Cadamuro, Giuseppe Lippi, Ana-Maria Simundic
The presence of interfering substances like free hemoglobin, bilirubin or lipids compromises sample quality and potentially affects laboratory analysis and test results. Recently, the use of serum indices for objectively assessing sample quality has become commonplace and many preanalytical platforms, as well as clinical chemistry and coagulation analyzers, are now equipped with this analytical feature. Nevertheless, some important drawbacks remain in this practice, mainly pertaining the measurement procedure, the approach for reporting interference data, the definition of objective thresholds of interference after which test results may be biased, and the lack of harmonized practices for describing how interference cut-offs have been identified...
April 1, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Robert Goggs, Antonio Borrelli, Benjamin M Brainard, Daniel L Chan, Armelle de Laforcade, Isabelle Goy-Thollot, Karl E Jandrey, Annemarie T Kristensen, Annette Kutter, Clara B Marschner, Benoît Rannou, Nadja Sigrist, Catherine Wagg
OBJECTIVE: To establish and compare the repeatability and reproducibility of activated thromboelastography (TEG) and thromboelastometry (ROTEM) assays. DESIGN: Multicenter in vitro test standardization. SETTING: Veterinary academic centers. ANIMALS: Test samples were obtained from normal, healthy dogs. Sixty identical 5 mL aliquots of canine platelet-rich plasma collected by apheresis, frozen in 6% dimethyl sulfoxide, were tested initially...
March 31, 2018: Journal of Veterinary Emergency and Critical Care
Rekha Vijay Kumar, Dipti Panwar, Usha Amirtham, Chennagiri Srinivasmurthy Premalata, Champaka Gopal, Suma Mysore Narayana, Geetha Vikram Patil Okaly, K C Lakshmaiah, S Krishnamurthy
Aim: The aim of the study was to analyze the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (HER2) status over 7 years in South Indian women with breast cancer. Further analysis of a subgroup was done to study clinically defined subtypes and the role of preanalytical factors in needle core biopsies (NCBs) and excised specimens. Materials and Methods: This was a retrospective study from January 2010 to December 2016...
January 2018: South Asian Journal of Cancer
Benoît Cuq, Shauna L Blois, R Darren Wood, Gabrielle Monteith, Anthony C Abrams-Ogg, Christian Bédard, Geoffrey A Wood
BACKGROUND: Thrombin plays a central role in hemostasis and thrombosis. Calibrated automated thrombography (CAT), a thrombin generation assay, may be a useful test for hemostatic disorders in dogs. OBJECTIVES: To describe CAT results in a group of healthy dogs, and assess preanalytical variables and biological variability. ANIMALS: Forty healthy dogs were enrolled. METHODS: Lag time (Lag), time to peak (ttpeak), peak thrombin generation (peak), and endogenous thrombin potential (ETP) were measured...
March 26, 2018: Veterinary Clinical Pathology
Ioannis L Oikonomidis, Christos Brozos, Evangelos Kiossis, Maria Kritsepi-Konstantinou
BACKGROUND: Clinical pathology investigations are essential to evaluate the health status and practice management of ruminants. Appropriate and accurate RIs are required for the interpretation of laboratory results. Despite substantial phenotypic differences and management-related disparities between male and female sheep, RIs in rams are sparse and limited by small reference populations and outdated methods. OBJECTIVES: The purpose of this study was to establish RIs for hematologic, biochemical, and hormonal analytes in adult rams using controlled preanalytic and analytic procedures, and to investigate whether breed-dependent partitioning of RIs is required...
March 2018: Veterinary Clinical Pathology
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