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Preanalytical

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https://www.readbyqxmd.com/read/29148824/biobanking-of-fresh-frozen-cancer-tissue-rna-is-stable-independent-of-tissue-type-with-less-than-1-hour-of-cold-ischemia
#1
Sang Yong Song, Jonghyun Jun, Miyeon Park, Seo Kyu Park, Wonju Choi, Kyunghee Park, Kee-Taek Jang, Myoyong Lee
BACKGROUND: The effects of preanalytical variables in tissue processing and storage periods on RNA quality of tissues have been well documented in each type of cancer. However, few studies have been performed on a comparative assessment of the impacts across different cancer tissues, even though it is well known that RNase activity is highly variable in various tissue types and RNase-rich tissues have been found to yield low-quality RNA. METHODS: We investigated the impacts of cold ischemia times and long-term storage on RNA integrity in various types of cancer tissue, which had been fresh-frozen and collected at the Samsung Medical Center Biobank...
November 17, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/29146056/comparison-of-her2-status-determination-methods-in-her2-2-patients-manual-fluorescent-in-situ-hybridization-fish-vs-dual-silver-enhanced-in-situ-hybridization-sish
#2
Burcin Pehlivanoglu, Gurdeniz Serin, Levent Yeniay, Osman Zekioglu, Erhan Gokmen, Necmettin Ozdemir
HER2 amplification has been demonstrated in 15-25% of invasive breast carcinomas and can be assessed using immunohistochemical and in situ hybridization methods. Here, we compared the accuracy of dual SISH to manual FISH in HER2 (2+) breast carcinoma and evaluated the feasibility of dual SISH method in routine practice. Sixty HER2 (2+) consecutive tumor samples diagnosed between January 2009 and February 2013 were selected. Demographic, histological and immunohistochemical features and FISH results were recruited from patient records and compared to dual SISH results...
December 2017: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/29144792/effect-of-blood-collection-tube-type-and-time-to-processing-on-the-enumeration-and-high-content-characterization-of-circulating-tumor-cells-using-the-high-definition-single-cell-assay
#3
Mariam Rodríguez-Lee, Anand Kolatkar, Madelyn McCormick, Angel D Dago, Jude Kendall, Nils Anders Carlsson, Kelly Bethel, Emily J Greenspan, Shelley E Hwang, Kathryn R Waitman, Jorge J Nieva, James Hicks, Peter Kuhn
CONTEXT: - As circulating tumor cell (CTC) assays gain clinical relevance, it is essential to address preanalytic variability and to develop standard operating procedures for sample handling in order to successfully implement genomically informed, precision health care. OBJECTIVE: - To evaluate the effects of blood collection tube (BCT) type and time-to-assay (TTA) on the enumeration and high-content characterization of CTCs by using the high-definition single-cell assay (HD-SCA)...
November 16, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/29137972/circulating-tumoral-dna-preanalytical-validation-and-quality-control-in-a-diagnostic-laboratory
#4
Sergey Nikolaev, Laure Lemmens, Thibaud Koessler, Jean-Louis Blouin, Thierry Nouspikel
We present the results of our technical validation process in establishing the analysis of circulating tumor DNA (ctDNA) as a diagnostic tool. Like most cells in our body, tumor cells shed DNA in the blood flow. Analysis of ctDNA mutational content can provide invaluable information on the genetic makeup of a tumor, and assist oncologists in deciding on therapy, or in following residual disease. However, low absolute amounts of circulating DNA and low tumor fraction constitute formidable analytical challenges...
November 11, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29115211/measurement-of-gastrointestinal-hormones
#5
Nicolai J Wewer Albrechtsen
Towards the end of the 20th century, the number of subjects with diabetes and obesity rose exponentially. The discoveries of insulin- and appetite-modulating chemical signals, including glucagon-like peptide-1 (GLP-1), secreted from the gastrointestinal system, led to development of a new group of drugs which now are being used for glucose-lowering therapy and weight loss. Understanding of the physiology of gut derived signals and their pathophysiologi-cal importance requires accurate measurements of their circulat-ing levels...
November 2017: Danish Medical Journal
https://www.readbyqxmd.com/read/29110777/the-use-of-cerebrospinal-fluid-in-biomarker-studies
#6
C E Teunissen, C Verheul, E A J Willemse
Cerebrospinal fluid (CSF) is an extremely useful matrix for biomarker research for several purposes, such as diagnosis, prognosis, monitoring, and identification of prominent leads in pathways of neurologic diseases. Such biomarkers can be identified based on a priori hypotheses around prominent protein changes, but also by applying -omics technologies. Proteomics is widely used, but metabolomics and transcriptomics are rapidly revealing their potential for CSF studies. The basis of such studies is the availability of high-quality biobanks...
2017: Handbook of Clinical Neurology
https://www.readbyqxmd.com/read/29106292/preanalytic-factors-associated-with-hemolysis-in-emergency-department-blood-samples
#7
Michael P Phelan, Edmunds Z Reineks, Jesse D Schold, Frederic M Hustey, Janelle Chamberlin, Gary W Procop
CONTEXT: - Hemolysis of emergency department blood samples is a common occurrence and has a negative impact on health care delivery. OBJECTIVES: - To determine the effect of preanalytic factors (straight stick, intravenous [IV] line, needle gauge, location of blood draw, syringe versus vacuum tube use, tourniquet time) on hemolysis in emergency department blood samples. DESIGN: - A single 65 000-visit emergency department's electronic health record was queried for emergency department potassium results and blood draw technique for all samples obtained in calendar year 2014, resulting in 54 531 potassium results...
November 6, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/29084061/tissue-thickness-effects-on-immunohistochemical-staining-intensity-of-markers-of-cancer
#8
Adrienne S McCampbell, Varun Raghunathan, May Tom-Moy, Richard K Workman, Rick Haven, Amir Ben-Dor, Ole F Rasmussen, Lars Jacobsen, Martin Lindberg, N Alice Yamada, Carol Schembri
High-quality patient samples are required for reliable immunohistochemistry test outcomes that provide a significant benefit for patient care. Among the preanalytic variables in tissue handling, tissue thickness is thought to be easily controlled; however, whether the thickness of the tissue effects the staining intensity for antibody immunohistochemistry has not been quantitatively demonstrated. To investigate, we cut multiblock tissue sections of tonsil, liver, and kidney at 2, 4, 6, and 8 μm thicknesses...
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29084060/characterization-of-pd-l1-immunohistochemical-expression-in-cell-blocks-with-different-specimen-fixation-and-processing-methods
#9
Isaac E Lloyd, Wenhua Zhou, Benjamin L Witt, Barbara E Chadwick
Interpretative criteria for programmed death-ligand 1 (PD-L1) immunohistochemistry (IHC) have been largely based on data from formalin-fixed, paraffin-embedded tissues, despite the fact that cytologic specimens, especially cell blocks, are often the only or most readily available tissue for testing. Unlike biopsy specimens, however, cytology sample processing methods can vary markedly. The purpose of this study was to evaluate the effects of several common preanalytic variables on PD-L1 IHC. Two cell lines with strong expression of PD-L1 (H441) and no expression (MCF7) were cultured in vitro...
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29065378/clinical-relevance-of-molecular-diagnostics-in-gastrointestinal-gi-cancer-european-society-of-digestive-oncology-esdo-expert-discussion-and-recommendations-from-the-17th-european-society-for-medical-oncology-esmo-world-congress-on-gastrointestinal-cancer-barcelona
#10
REVIEW
Alexander Baraniskin, Jean-Luc Van Laethem, Lucjan Wyrwicz, Ulrich Guller, Harpreet S Wasan, Tamara Matysiak-Budnik, Thomas Gruenberger, Michel Ducreux, Fatima Carneiro, Eric Van Cutsem, Thomas Seufferlein, Wolff Schmiegel
BACKGROUND AND SCOPE: In the epoch of precision medicine and personalised oncology, which aims to deliver the right treatment to the right patient, molecular genetic biomarkers are a topic of growing interest. The aim of this expert discussion and position paper is to review the current status of various molecular tests for gastrointestinal (GI) cancers and especially considering their significance for the clinical routine use. METHODOLOGY: Opinion leaders and experts from diverse nationalities selected on scientific merit were asked to answer to a prepared set of questions about the current status of molecular diagnostics in different GI cancers...
November 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/29055897/t-cell-clonality-assessment-past-present-and-future
#11
REVIEW
Etienne Mahe, Tevor Pugh, Suzanne Kamel-Reid
T cell clonality testing has important clinical and research value, providing a specific and reproducible assessment of clonal diversity in T cell proliferations. Here we review the conceptual foundations of T cell clonality assays, including T cell ontogeny and T cell receptor structure and function; we also provide an introduction to T cell receptor genomics and the concept of the T cell clonotype. This is followed by a review of historical and current methods by which T cell clonality may be assayed, including current assay limitations...
October 21, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29048424/analysis-of-human-cerebrospinal-fluid-monoamines-and-their-cofactors-by-hplc
#12
Marta Batllori, Marta Molero-Luis, Aida Ormazabal, Mercedes Casado, Cristina Sierra, Angels García-Cazorla, Manju Kurian, Simon Pope, Simon J Heales, Rafael Artuch
The presence of monoamines and their cofactors (the pterins and vitamin B6 (pyridoxal phosphate (PLP))) in human cerebrospinal fluid (CSF) can be used as indicators of the biosynthesis and turnover of dopamine and serotonin in the brain. In addition, abnormalities in the CSF levels of these molecules are associated with various neurological diseases, including genetic diseases leading to dopamine and serotonin deficiency. Here, we provide a set of quantitative high-performance liquid-chromatography (HPLC) approaches to determine CSF levels of monoamines and their cofactors...
November 2017: Nature Protocols
https://www.readbyqxmd.com/read/29045515/performance-of-the-cobas-hpv-test-for-the-triage-of-atypical-squamous-cells-of-undetermined-significance-cytology-in-cervical-specimens-collected-in-surepath
#13
Devansu Tewari, Susan Novak-Weekley, Christina Hong, Shagufta Aslam, Catherine M Behrens
Objectives: Determine performance of the cobas human papillomavirus (HPV) test for triage of atypical squamous cells of undetermined significance (ASC-US) in SurePath. Methods: Women presenting for routine screening had cervical specimens collected in SurePath and specimen transport medium (STM); those with ASC-US cytology underwent colposcopy. Performance of cobas HPV in SurePath specimens that had undergone a preanalytic procedure to reverse possible cross-linking of HPV DNA was compared with Hybrid Capture 2 (hc2) specimens in STM...
November 2, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29043982/preanalytical-influence-of-pneumatic-tube-delivery-system-on-results-of-routine-biochemistry-and-haematology-analysis
#14
Morgane Petit, Louis Mine, Tiffany Pascreau, Chantal Brouzes, Sandrine Majoux, Delphine Borgel, Jean-Louis Beaudeux, Dominique Lasne, Carole Hennequin
Pneumatic tube delivery system (PTS) enables to reduce considerably turnaround times. The aim of the study was to assess the influence of the PTS on the quality of routine biochemical and hematological tests in our laboratory. Blood samples from 6 hospitalized patients and 8 healthy volunteers were analyzed. Blood samples were delivered to the laboratory by a PTS and by a human courier. We performed the following analysis: ionized calcium, sodium, potassium, lactate deshydrogenase (LDH), aspartate aminotransferase (ASAT), arterial blood gas, complete blood count and coagulation test as prothrombin time, activated partial thromboplastin time, factors V and VIII...
October 18, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/29031016/preanalytics-of-ammonia-stability-transport-and-temperature-of-centrifugation
#15
Julien Favresse, Noémie Despas, Lidvine Boland, Damien Gruson, Pierre-Françoise Laterre, Marie-Cécile Nassogne, Marie-Françoise Vincent, Catherine Fillée
No abstract text is available yet for this article.
October 14, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28984289/preanalytical-analytical-and-postanalytical-errors-in-the%C3%A2-measurement-of-irisin-levels-authors-reply
#16
Małgorzata Kałużna, Krzysztof Hoppe, Krzysztof Schwermer, Aisha Y Ibrahim, Krzysztof Pawlaczyk, Katarzyna Ziemnicka
No abstract text is available yet for this article.
September 29, 2017: Polish Archives of Internal Medicine
https://www.readbyqxmd.com/read/28984288/preanalytical-analytical-and-postanalytical-errors-in-the%C3%A2-measurement-of-irisin-levels
#17
Mehmet Kalayci
No abstract text is available yet for this article.
September 29, 2017: Polish Archives of Internal Medicine
https://www.readbyqxmd.com/read/28969112/pre-analytical-errors-at-the-chemical-pathology-laboratory-of-a-teaching-hospital
#18
Marlene A Tapper, James C Pethick, Lowell L Dilworth, Donovan A McGrowder
INTRODUCTION: The Chemical Pathology Laboratory at the University Hospital of the West Indies (UHWI) processes specimens received from inpatients, the outpatient department and other medical facilities in Jamaica. Specific rejection criteria are used to determine samples unsuitable for analysis. It has been noted that despite efforts to reduce the number of unacceptable samples received in the laboratory, the problem persists. AIM: The study seeks to provide empirical evidence of the inadequacies from which improvements can be formulated...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28967956/variability-and-error-in-cardiac-troponin-testing-an-aclps-critical-review
#19
REVIEW
Daniel S Herman, Peter A Kavsak, Dina N Greene
Objectives: To provide a comprehensive overview of the complexities associated with cardiac troponin (cTn) testing. An emphasis is placed on the sources of error, organized into the preanalytical, analytical, and postanalytical phases of the testing pathway. Controversial areas are also explored. Methods: A case scenario and review of the relevant literature describing laboratory considerations involving cTn testing are described. Results: Advanced comprehension of the specific assay used in a given laboratory is necessary for optimal reporting, utilization, and quality monitoring of cTn...
October 1, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28967954/deviation-management-key-management-subsystem-driver-of-knowledge-based-continuous-improvement-in-the-henry-ford-production-system
#20
Richard J Zarbo, Jacqueline R Copeland, Ruan C Varney
Objectives: To develop a business subsystem fulfilling International Organization for Standardization 15189 nonconformance management regulatory standard, facilitating employee engagement in problem identification and resolution to effect quality improvement and risk mitigation. Methods: From 2012 to 2016, the integrated laboratories of the Henry Ford Health System used a quality technical team to develop and improve a management subsystem designed to identify, track, trend, and summarize nonconformances based on frequency, risk, and root cause for elimination at the level of the work...
October 1, 2017: American Journal of Clinical Pathology
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