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A20 AND infection

Katie E Crump, Jennifer C Oakley, Xia Xia-Juan, Theandra C Madu, Swathi Devaki, Erin C Mooney, Sinem E Sahingur
TLR9 deficient (TLR9(-/-)) mice are resistant to periodontitis, a disease characterized by a dysbiotic microbiota and deregulated immune response resulting in tooth loss and various systemic conditions. However, the mechanisms and biological pathways by which TLR9 instigates periodontal inflammation are yet to be identified. In a ligature-induced model of periodontitis, we demonstrate TLR9(-/-) mice exhibited significantly less alveolar bone loss compared to their wild-type (WT) counterparts. Consistent with the disease phenotype, gingival tissues showed significantly more inflammatory cell infiltrate in the WT ligated but not in the TLR9(-/-) ligated mice compared to their unligated controls...
November 14, 2016: Infection and Immunity
Haihua Li, Lei Zhang, Longbin Chen, Qi Zhu, Wenjie Wang, Jiayun Qiao
BACKGROUND: A newly isolated L. acidophilus strain has been reported to have potential anti-inflammatory activities against lipopolysaccharide (LPS) challenge in piglet, while the details of the related inflammatory responses are limited. Here we aimed to analysis the ability of L. acidophilus to regulate inflammatory responses and to elucidate the mechanisms involved in its anti-inflammatory activity. RESULTS: The ETEC (enterotoxigenic Escherichia coli) K88-induced up-regulations of IL-1β, IL-8 and TNF-α were obviously inhibited by L...
November 10, 2016: BMC Microbiology
Xin Yao, Lian-Lian Bian, Wei-Wei Lu, Jing-Xin Li, Qun-Ying Mao, Yi-Ping Wang, Fan Gao, Xing Wu, Qiang Ye, Xiu-Ling Li, Feng-Cai Zhu, Zhenglun Liang
Herpangina (HA) and hand, foot, and mouth disease (HFMD) are common infectious diseases caused by human enteroviruses and frequently occurr in young children. Previous published studies have mainly focused on HFMD, while the HA epidemiological and etiological characteristics in mainland China have not been described. From June, 2013 to March, 2014, HA and HFMD patients were monitored in participants from clinical trial of EV-A71 vaccine conducted during 2012-2013. A total of 95 HA patients and 161 HFMD patients were defined...
October 21, 2016: Human Vaccines & Immunotherapeutics
Yu-Chen Fan, Yuan-Yuan Zhang, Yan-Yan Sun, Na Wang, Xiao-Yan Xiao, Kai Wang
A20 is an important negative immune regulator but its role in chronic hepatitis B virus (HBV) infection is still unknown. This present study was to investigate the potential role of A20 gene in the progression of chronic HBV infection. A total of 236 chronic HBV patients were included and consisted of 63 hepatocellular carcinoma (HCC), 87 liver cirrhosis (LC) and 86 chronic hepatitis B (CHB). The mRNA level of A20 gene in peripheral blood mononuclear cells was determined using quantitative real-time polymerase chain reaction...
September 13, 2016: Oncotarget
Ning Liu, Lin Wang, Changjiang Sun, Li Yang, Wanchun Sun, Qisheng Peng
BACKGROUND: Brucella may establish chronic infection by regulating the expression of miRNAs. However, the role of miRNAs in modulating the intracellular growth of Brucella remains unclear. RESULTS: In this study, we show that Brucella. abortus infection leads to downregulation of miR-125b-5p in macrophages. We establish that miR-125b-5p targets A20, an inhibitor of the NF-kB activation. Additionally, expression of miR-125b-5p decreases A20 expression in B. abortus-infected macrophages and leads to NF-kB activation and increased production of TNFα...
2016: BMC Microbiology
Fikret Aydemir, Maxim Salganik, Justyna Resztak, Jasbir Singh, Antonette Bennett, Mavis Agbandje-McKenna, Nicholas Muzyczka
UNLABELLED: We previously reported that an amino acid substitution, Y704A, near the 2-fold interface of adeno-associated virus (AAV) was defective for transcription of the packaged genome (M. Salganik, F. Aydemir, H. J. Nam, R. McKenna, M. Agbandje-McKenna, and N. Muzyczka, J Virol 88:1071-1079, 2013, doi: In this report, we have characterized the defect in 6 additional capsid mutants located in a region ∼30 Å in diameter on the surface of the AAV type 2 (AAV2) capsid near the 2-fold interface...
August 15, 2016: Journal of Virology
Xiaotian Song, Zhiyan Yao, Jianling Yang, Zhengzheng Zhang, Yuqing Deng, Miao Li, Cuiqing Ma, Lijuan Yang, Xue Gao, Wenjian Li, Jianguo Liu, Lin Wei
Hepatitis C virus (HCV) infection is characterized by a strong propensity toward chronicity. During chronic HCV infection, HCV core protein is implicated in deregulating cytokine expression that associates with chronic inflammation. A20 is known as a powerful suppressor in cytokine signaling, in this study, we explored the A20 expression in macrophages induced by HCV core protein and the involved signaling pathways. Results demonstrated that HCV core protein induced A20 expression in macrophages. Silencing A20 significantly enhanced the secretion of IL-6, IL-1β and TGF-β1, but not IL-8 and TNF...
June 7, 2016: Oncotarget
Sonal Tuli, Matthew Gray
PURPOSE OF REVIEW: The purpose of this review is to discuss the options for, and recent developments in, the surgical treatment of corneal infections. Although the mainstay of treatment of corneal infections is topical antimicrobial agents, surgical intervention may be necessary in a number of cases. These include advanced disease at presentation, resistant infections, and progressive ulceration despite appropriate treatment. Prompt and appropriate treatment can make the difference between a good outcome and loss of vision or the eye...
July 2016: Current Opinion in Ophthalmology
Emmanuel Asare, JoAnn Mugavero, Ping Jiang, Eckard Wimmer, Aniko V Paul
UNLABELLED: The specificity of encapsidation of C-cluster enteroviruses depends on an interaction between capsid proteins and nonstructural protein 2C(ATPase) In particular, residue N252 of poliovirus 2C(ATPase) interacts with VP3 of coxsackievirus A20, in the context of a chimeric virus. Poliovirus 2C(ATPase) has important roles both in RNA replication and encapsidation. In this study, we searched for additional sites in 2C(ATPase), near N252, that are required for encapsidation. Accordingly, segments adjacent to N252 were analyzed by combining triple and single alanine mutations to identify residues required for function...
July 15, 2016: Journal of Virology
Takamasa Ishizuka, Paulraj Kanmani, Hisakazu Kobayashi, Ayako Miyazaki, Junichi Soma, Yoshihito Suda, Hisashi Aso, Tomonori Nochi, Noriyuki Iwabuchi, Jin-zhong Xiao, Tadao Saito, Julio Villena, Haruki Kitazawa
In this work, we aimed to characterize the antiviral response of an originally established porcine intestinal epithelial cell line (PIE cells) by evaluating the molecular innate immune response to rotavirus (RVs). In addition, we aimed to select immunomodulatory bacteria with antiviral capabilities. PIE cells were inoculated with RVs isolated from different host species and the infective titers and the molecular innate immune response were evaluated. In addition, the protection against RVs infection and the modulation of immune response by different lactic acid bacteria (LAB) strains was studied...
2016: PloS One
Gippeum Joy Lee, Hye-Mi Lee, Tae Sung Kim, Jin Kyung Kim, Kyung Mok Sohn, Eun-Kyeong Jo
Mycobacterium fortuitum is a rapidly growing mycobacterium that has been regarded as an etiological agent of a variety of human infections. However, little is known about the host inflammatory responses and the molecular mechanisms by which MF-induced inflammation is regulated in macrophages. In this study, we report that MF infection leads to the induction of an anti-inflammatory molecule, A20 (also known as TNFAIP3), which is essential for the regulation of MF-induced inflammatory responses in murine bone marrow-derived macrophages (BMDMs)...
April 2016: Pathogens and Disease
Kota Okadera, Masako Abe, Naoto Ito, Hiromichi Mitake, Kazuma Okada, Kento Nakagawa, Yumi Une, Hiroshi Tsunemitsu, Makoto Sugiyama
To estimate the risk of interspecies transmission of rotavirus species A (RVA) from exotic pets to other mammalian species, the prevalence of RVA in sugar gliders (Petaurus breviceps) was investigated. RVAs were detected in 10 of 44 sugar gliders by reverse transcription (RT)-semi-nested PCR. These viruses were classified as G27P[3] and G27P[36] genotypes, with G27 and P[36] being new genotypes as assigned by the Rotavirus Classification Working Group. To characterize sugar glider RVA in detail, one strain, RVA/SugarGlider-tc/JPN/SG385/2012/G27P[36] (SG385-tc), was isolated...
May 2016: Journal of General Virology
Katharina Wex, Ursula Schmid, Sissy Just, Xu Wang, Rebecca Wurm, Michael Naumann, Dirk Schlüter, Gopala Nishanth
Upon infection with intracellular bacteria, nucleotide oligomerization domain protein 2 recognizes bacterial muramyl dipeptide and binds, subsequently, to receptor-interacting serine/threonine kinase 2 (RIPK2), which activates immune responses via the nuclear factor kappa-light-chain enhancer of activated B cells (NF-κB) and extracellular signal-regulated kinase (ERK) pathways. Activation of RIPK2 depends on its K63 ubiquitination by E3 ligases, whereas the deubiquitinating enzyme A20 counter regulates RIPK2 activity by cleaving K63-polyubiquitin chains from RIPK2...
2015: Frontiers in Immunology
Jonathan Maelfait, Kenny Roose, Lars Vereecke, Conor Mc Guire, Mozes Sze, Martijn J Schuijs, Monique Willart, Lorena Itati Ibañez, Hamida Hammad, Bart N Lambrecht, Rudi Beyaert, Xavier Saelens, Geert van Loo
A20 negatively regulates multiple inflammatory signalling pathways. We here addressed the role of A20 in club cells (also known as Clara cells) of the bronchial epithelium in their response to influenza A virus infection. Club cells provide a niche for influenza virus replication, but little is known about the functions of these cells in antiviral immunity. Using airway epithelial cell-specific A20 knockout (A20AEC-KO) mice, we show that A20 in club cells critically controls innate immune responses upon TNF or double stranded RNA stimulation...
January 2016: PLoS Pathogens
Fernanda Fredericksen, Melina Villalba, Víctor H Olavarría
Cytokine production for immunological process is tightly regulated at the transcriptional and posttranscriptional levels. The NF-κB signaling pathway maintains immune homeostasis in the cell through the participation of molecules such as A20 (TNFAIP3), which is a key regulatory factor in the immune response, hematopoietic differentiation, and immunomodulation. Although A20 has been identified in mammals, and despite recent efforts to identify A20 members in other higher vertebrates, relatively little is known about the composition of this regulator in other classes of vertebrates, particularly for bovines...
May 1, 2016: Gene
Xiao-Xin Ren, Hai-Bo Wang, Chuan Li, Jin-Feng Jiang, Si-Dong Xiong, Xia Jin, Li Wu, Jian-Hua Wang
HIV-1 depends on host-cell-encoded factors to complete its life cycle. A comprehensive understanding of how HIV-1 manipulates host machineries during viral infection can facilitate the identification of host targets for antiviral drugs or gene therapy. The cellular protein Naf1 (HIV-1 Nef-associated factor 1) is a CRM1-dependent nucleo-cytoplasmic shuttling protein, and has been identified to regulate multiple receptor-mediated signal pathways in inflammation. The cytoplasm-located Naf1 can inhibit NF-κB activation through binding to A20, and the loss of Naf1 controlled NF-κB activation is associated with multiple autoimmune diseases...
February 26, 2016: Journal of Biological Chemistry
Yan-Yan Sun, Yu-Chen Fan, Na Wang, Harry Hua-Xiang Xia, Xiao-Yan Xiao, Kai Wang
The zinc finger protein A20 is a newly identified negative regulator of immune response and mediates signal pathway of NF-κB in liver inflammation. However, the role of A20 in the natural history of patients with chronic hepatitis B (CHB) has not been demonstrated. In this present study, we aimed to investigate the dynamic expression of A20 and determine the potential association of A20 in the progression of chronic hepatitis B virus infection.This retrospective study contained 136 patients with chronic hepatitis B and 30 healthy controls (HCs)...
December 2015: Medicine (Baltimore)
Y Saitoh, A Hamano, K Mochida, A Kakeya, M Uno, E Tsuruyama, H Ichikawa, F Tokunaga, A Utsunomiya, T Watanabe, S Yamaoka
Adult T-cell leukemia (ATL) arises from a human T-cell leukemia virus type I (HTLV-I)-infected cell and has few therapeutic options. Here, we have uncovered a previously unrecognized role for a ubiquitin-editing enzyme A20 in the survival of HTLV-I-infected cells. Unlike in lymphomas of the B-cell lineage, A20 is abundantly expressed in primary ATL cells without notable mutations. Depletion of A20 in HTLV-I-infected cells resulted in caspase activation, cell death induction and impaired tumorigenicity in mouse xenograft models...
March 2016: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
Chao Fan, Ying Zhang, Yun Zhou, Bingjie Li, Yu He, Yonghong Guo, Zhansheng Jia
BACKGROUND: Anti-hepatitis C virus (HCV) responses are often accompanied by an increase in alanine aminotransferase levels in HCV-infected patients, indicating that inflammatory responses are compromised by the virus. Additionally, inflammation is associated with M1-polarizated macrophages, which secrete cytokines such as tumor necrosis factor-α, interleukin-1, and interleukin-12, and present antigens through phagocytosis. HCV-encoded proteins are presented as specific viral antigens in particular infectious steps that influence the immune response...
September 17, 2015: Virology Journal
Parimal Roy, A F M Fakhrul Islam, Susan K Burgess, Peter W Hunt, Jody McNally, Stephen W Walkden-Brown
Infectious laryngotracheitis (ILT) is an important disease of chickens caused by ILT virus (ILTV). We used the Australian SA2 and A20 vaccine strains of ILTV to determine tissue distribution and excretion characteristics of ILTV in specific-pathogen-free chickens and to determine whether ILTV is readily detectable in environmental samples such as faeces, bedding material and dust using real-time quantitative PCR. Three groups of 10 freshly hatched chicks were placed in isolators and infected orally with high doses of the two strains of vaccine virus or left unchallenged as controls...
November 2015: Journal of General Virology
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