keyword
MENU ▼
Read by QxMD icon Read
search

"tissue culture"

keyword
https://www.readbyqxmd.com/read/28933036/resistance-genes-in-piper-colubrinum-in-silico-survey-from-leaf-transcriptome-and-expression-studies-upon-challenge-inoculation-with-phytophthora-capsici
#1
Neema Malik, Johnson K George
The oomycetes, Phytophthora capsici, cause foot rot disease in black pepper. Piper colubrinum Link, a distant relative of cultivated black pepper, is highly resistant to this destructive pathogen. Identification of resistance (R) genes in P. colubrinum and the study of its expression profile during interaction with the pathogen can help in understanding the resistance mechanism involved. In the present study, 1289 R gene-related transcripts were mined from P. colubrinum transcriptome, clustered, and classified according to the conserved motifs and domains...
September 20, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28930445/thermo-responsive-and-active-functional-fiber-mats-for-cultured-cell-recovery
#2
Wilaiporn Graisuwan, Songchan Puthong, Hui Zhao, Suda Kiatkamjornwong, Patrick Theato, Voravee P Hoven
Thermo-responsive and active functional fiber mats were prepared from random copolymer of poly(pentafluorophenyl acrylate-co-N-isopropylacrylamide) (P(PFPA-co-NIPAM)), which was synthesized by a controlled radical polymerization process based on reversible addition-fragmentation chain transfer (RAFT). As reactive sites, pentafluorophenyl ester (PFP) groups were incorporated in the copolymer to allow for a multiple post-polymerization modification. UV-crosslinkable moieties were first introduced by partially reacting PFP groups in the copolymer with ortho-nitrobenzyl (ONB)-protected diamine...
September 20, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28928753/peg-induces-high-expression-of-the-cell-cycle-checkpoint-gene-wee1-in-embryogenic-callus-of-medicago-truncatula-potential-link-between-cell-cycle-checkpoint-regulation-and-osmotic-stress
#3
Adel M Elmaghrabi, Hilary J Rogers, Dennis Francis, Sergio J Ochatt
Polyethylene glycol (PEG) can be used to mimic osmotic stress in plant tissue cultures to study mechanisms of tolerance. The aim of this experiment was to investigate the effects of PEG (M.W. 6000) on embryogenic callus of Medicago truncatula. Leaf explants were cultured on MS medium with 2 mg L(-1) NAA and 0.5 mg L(-1) BAP for 5 months. Then, calli were transferred to the same medium further supplemented with 10% (w/v) 6000 PEG for 6 months in order to study physiological and putative molecular markers of water stress...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28924608/a-versatile-3d-tissue-matrix-scaffold-system-for-tumor-modeling-and-drug-screening
#4
Girdhari Rijal, Weimin Li
Most of the anticancer drug candidates entering preclinical trials fail to be approved for clinical applications. The following are among the main causes of these failures: studying molecular mechanisms of cancer development, identifying therapeutic targets, and testing drug candidates using inappropriate tissue culture models, which do not recapitulate the native microenvironment where the cancer cells originate. It has become clear that three-dimensional (3D) cell cultures are more biologically and clinically relevant than 2D models...
September 2017: Science Advances
https://www.readbyqxmd.com/read/28923507/pentagalloylglucose-a-highly-bioavailable-polyphenolic-compound-present-in-cortex-moutan-efficiently-blocks-hepatitis-c-virus-entry
#5
Patrick Behrendt, Paula Perin, Nicolas Menzel, Dominic Banda, Stephanie Pfaender, Marco P Alves, Volker Thiel, Phillip Meulemann, Che C Colpitts, Luis M Schang, Florian W R Vondran, Anggakusuma, Michael P Manns, Eike Steinmann, Thomas Pietschmann
Approximately 142 million people worldwide are infected with hepatitis C virus (HCV). Although potent direct acting antivirals are available, high costs limit access to treatment. Chronic hepatitis C virus infection remains a major cause of orthotopic liver transplantation. Moreover, re-infection of the graft occurs regularly. Antivirals derived from natural sources might be an alternative and cost-effective option to complement therapy regimens for global control of hepatitis C virus infection. We tested the antiviral properties of a mixture of different Chinese herbs/roots named Zhi Bai Di Huang Wan (ZBDHW) and its individual components on HCV...
September 15, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28923015/transcriptome-analysis-of-pseudostellaria-heterophylla-in-response-to-the-infection-of-pathogenic-fusarium-oxysporum
#6
Xianjin Qin, Hongmiao Wu, Jun Chen, Linkun Wu, Sheng Lin, Muhammad Umar Khan, Mohammad Reza Boorboori, Wenxiong Lin
BACKGROUND: Pseudostellaria heterophylla (P. heterophylla), a herbaceous perennial, belongs to Caryophyllaceae family and is one of the Chinese herbal medicine with high pharmacodynamic value. It can be used to treat the spleen deficiency, anorexia, weakness after illness and spontaneous perspiration symptoms. Our previous study found that consecutive monoculture of Pseudostellaria heterophylla could lead to the deterioration of the rhizosphere microenvironment. The specialized forms of pathogenic fungus Fusarium oxysporum f...
September 18, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28921542/pathogen-inactivation-of-dengue-virus-in-red-blood-cells-using-amustaline-and-glutathione
#7
Maite Aubry, Andrew Laughhunn, Felicia Santa Maria, Marion C Lanteri, Adonis Stassinopoulos, Didier Musso
BACKGROUND: Dengue virus (DENV) is an arbovirus primarily transmitted through mosquito bite; however, DENV transfusion-transmitted infections (TTIs) have been reported and asymptomatic DENV RNA-positive blood donors have been identified in endemic countries. DENV is considered a high-risk pathogen for blood safety. One of the mitigation strategies to prevent arbovirus TTIs is pathogen inactivation. In this study we demonstrate that the amustaline and glutathione (S-303/GSH) treatment previously found effective against Zika virus in red blood cells (RBCs) is also effective in inactivating DENV...
September 16, 2017: Transfusion
https://www.readbyqxmd.com/read/28913804/doubled-haploid-laboratory-protocol-for-wheat-using-wheat-maize-wide-hybridization
#8
Meenakshi Santra, Hong Wang, Scott Seifert, Scott Haley
In traditional wheat breeding, the uniformity of lines derived from a breeding population is obtained by repeated selfing from the F1 which takes several generations to reach homozygosity in loci controlling traits of interest. Using doubled haploid technology, however, it is possible to attain 100% homozygosity at all loci in a single generation and completely homogeneous breeding lines can be obtained in 1-2 years. Thus, doubled haploid technology may significantly reduce cultivar development time. Two major methods for producing wheat doubled haploids are androgenesis (anther culture and microspore culture) and embryo culture using wheat-maize wide hybridization, the latter being the most effective and widely used method...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28913800/wheat-genetic-transformation-using-mature-embryos-as-explants
#9
Harsh Chauhan, Paramjit Khurana
Feeding the growing population utilizing the limited agricultural resources remains a great challenge. Plant biotechnology plays a vital role in crop improvement by incorporating desired quality traits, tolerance to abiotic stresses and resistance to biotic stresses, which are otherwise tough to achieve by conventional plant breeding methodologies. Genetic engineering is a powerful tool to develop desired traits in selected crops to make the crops suitable for future demand and environment. For genetic engineering of crops like wheat, the development of efficient transformation and regeneration systems has always been a prime requirement...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28912069/generation-of-a-novel-human-cytomegalovirus-bacterial-artificial-chromosome-tailored-for-transduction-of-exogenous-sequences
#10
William L Close, Amit Bhandari, Marwa Hojeij, Philip E Pellett
The study of herpesviruses, including human cytomegalovirus (HCMV), is complicated by viral genome complexity and inefficient methods for genetic manipulation in tissue culture. Reverse genetics of herpesviruses has been facilitated by propagating their genomes in E. coli as bacterial artificial chromosomes (BACs), which enables complex and precise genetic manipulation using bacterial recombinational systems. Internal capsid volume imposes a strict limit on the length of genome that can be packaged efficiently...
September 11, 2017: Virus Research
https://www.readbyqxmd.com/read/28911623/a-comparative-study-of-three-tissue-cultured-dendrobium-species-and-their-wild-correspondences-by-headspace-gas-chromatography-mass-spectrometry-combined-with-chemometric-methods
#11
Nai-Dong Chen, Tao You, Jun Li, Li-Tao Bai, Jing-Wen Hao, Xiao-Yuan Xu
Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences. In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn...
October 2016: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28911258/effect-of-cryopreservation-on-cell-laden-hydrogels-comparison-of-different-cryoprotectants
#12
Nicola Cagol, Walter Bonani, Devid Maniglio, Claudio Migliaresi, Antonella Motta
Cell encapsulation in hydrogels is a technique that offers a variety of applications, ranging from drug delivery to biofabrication of three-dimensional scaffolds. The assembly of cell-laden hydrogel building blocks aims to generate complex biological constructs by manipulating microscale units. An important issue for the clinical implementation of this technique is the long-term storage of a large stock of cell/hydrogel building blocks. In this work, the impact of cryopreservation on the viability and functionality of cells encapsulated in alginate matrices is presented comparing different cryoprotective agents (CPAs)...
September 14, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28905518/production-of-a-novel-medium-chain-length-poly-3-hydroxyalkanoate-using-unprocessed-biodiesel-waste-and-its-evaluation-as-a-tissue-engineering-scaffold
#13
Pooja Basnett, Barbara Lukasiewicz, Elena Marcello, Harpreet K Gura, Jonathan C Knowles, Ipsita Roy
This study demonstrated the utilization of unprocessed biodiesel waste as a carbon feedstock for Pseudomonas mendocinaCH50, for the production of PHAs. A PHA yield of 39.5% CDM was obtained using 5% (v/v) biodiesel waste substrate. Chemical analysis confirmed that the polymer produced was poly(3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate) or P(3HHx-3HO-3HD-3HDD). P(3HHx-3HO-3HD-3HDD) was further characterized and evaluated for its use as a tissue engineering scaffold (TES)...
September 14, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28901663/antifungal-and-mechanical-properties-of-tissue-conditioner-containing-plant-derived-component-an-in-vitro-study
#14
Yoshihito Naito, Hiromichi Yumoto, Kiran Kumar Hs, Takashi Matsuo, Katsuhiko Hirota, Yoichiro Miyake, Kan Nagao, Yoritoki Tomotake, Ryo Jimbo, Tetsuo Ichikawa
PURPOSE: To evaluate the antifungal activity and mechanical properties of a novel antifungal tissue conditioner containing Juncus powder. MATERIALS AND METHODS: Juncus powder was mixed with GC tissue conditioner at concentrations of 2.5%, 5.0%, and 10.0% by mass. The cylindrical specimens of Juncus-mixed tissue conditioner (dimensions: 10 mm in diameter and 2 and 6 mm in height for antimicrobial and mechanical tests, respectively) were prepared. The specimens placed on the bottom of the 24-well tissue culture plate were cultured with Candida albicans CAD1 for 2 and 4 days...
September 13, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/28891951/analysis-of-class-i-major-histocompatibility-complex-gene-transcription-in-human-tumors-caused-by-human-papillomavirus-infection
#15
Steven F Gameiro, Ali Zhang, Farhad Ghasemi, John W Barrett, Anthony C Nichols, Joe S Mymryk
Oncoproteins from high-risk human papillomaviruses (HPV) downregulate the transcription of the class I major histocompatibility complex (MHC-I) antigen presentation apparatus in tissue culture model systems. This could allow infected or transformed cells to evade the adaptive immune response. Using data from over 800 human cervical and head & neck tumors from The Cancer Genome Atlas (TCGA), we determined the impact of HPV status on the mRNA expression of all six MHC-I heavy chain genes, and the β2 microglobulin light chain...
September 10, 2017: Viruses
https://www.readbyqxmd.com/read/28889732/effects-of-water-extract-of-curcuma-longa-l-roots-on-immunity-and-telomerase-function
#16
Min-Hsiung Pan, Jia-Ching Wu, Chi-Tang Ho, Vladimir Badmaev
Background Immunity and Longevity Methods A water extract of Curcuma longa (L.) [vern. Turmeric] roots (TurmericImmune™) standardized for a minimum 20 % of turmeric polysaccharides ukonan A, B, C and D was evaluated for its biological properties in in vitro tissue culture studies. Results The water extract of turmeric (TurP) exhibited induced-nitric oxide (NO) production in RAW264.7 macrophages. These results suggested the immunomodulatory effects of TurP. In addition, the polysaccharides up-regulated function of telomerase reverse transcriptase (TERT) equally to the phenolic compound from turmeric, curcumin...
May 12, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28888007/nano%C3%A2-calcium-phosphate-bone-cement-based-on-si-stabilized-%C3%AE-tricalcium-phosphate-with-improved-mechanical-properties
#17
M Roozbahani, M Alehosseini, M Kharaziha, R Emadi
This study aimed to develop nano‑calcium phosphate cement (nCPC) and evaluate the effect of nanosized precursors on mechanical, physical and handling properties (injectability and setting time) as well as conversion rate of nano-reactants into nano-hydroxyapatite (nHA). In this study, while alpha tricalcium phosphate (α-TCP, 98wt%) and HA (2wt%) were applied as the powder phase, 2.5wt% NaH2PO4 solution was used as liquid phase of cement. Before nano-CPC preparation, Si-stabilized α-TCP nanopowder with particle size of 10±3...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28887333/rapid-estrogen-receptor-alpha-signaling-mediated-by-erk-activation-regulates-vascular-tone-in-male-and-ovary-intact-female-mice
#18
Seong Chul Kim, Austin C Boese, Matthew H Moore, Rea M Cleland, Lin Chang, Patrice Delafontaine, Ke-Jie Yin, Jean-Pyo Lee, Milton H Hamblin
Estrogen has been shown to affect vascular reactivity. Here, we assessed estrogen receptor-alpha (ERα) dependency of estrogenic effects on vasorelaxation via a rapid nongenomic pathway in both male and ovary-intact female mice. We compared the effect of a primary estrogen, 17 beta-estradiol (E2) or 4,4',4"-(4-propyl-[1H]pyrazole-1,3,5-triyl)tris-phenol (PPT) (selective ERα agonist). We found that E2 and PPT induced greater aortic relaxation in females than males, indicating ERα mediation, which is further validated by employing ERα antagonism...
September 8, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28887084/tethered-growth-factors-on-biocompatible-scaffolds-improve-stemness-of-cultured-rat-and-human-neural-stem-cells-and-growth-of-oligodendrocyte-progenitors
#19
Lisamarie Moore, Nolan B Skop, Deborah E Rothbard, Lucas R Corrubia, Steven W Levison
Currently, there is no widely accepted technique to efficiently and reproducibly grow stem and progenitor cells in vitro. Stem cells require contact with extracellular matrices as well as signals from growth factors to proliferate and to retain their stemness. We have developed a novel tissue culture platform (StemTrix cultureware) that transforms standard tissue culture plasticware into a multi-functional chitosan-based scaffold that supports the expansion of neural stem cells. The StemTrix scaffold is comprised of chitosan with immobilized heparin which in turn tethers heparin-binding growth factors...
September 5, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28886311/effect%C3%A2-of%C3%A2-sucrose-concentrations-on-stevia-rebaudiana-bertoni-tissue-culture-and-gene-expression
#20
T Ghorbani, D Kahrizi, M Saeidi, I Arji
Stevia rebaudiana (Bert.) Bertoni is known as sweet plant which it contains a high level of steviol glycosides in the leaves.  This plant has been used from centuries ago as a sweetener for tea. One of the most important steviol glycosides is stevioside that is attractive for diabetic persons. Tissue culture is the only rapid process for the mass propagation of stevia. One of the most important factors in the medium is sucrose that is a necessary for plant growth. In the present study, we use nodal segments of the stem as explants in mediums with different sucrose concentration (50 mM, 100mM and 150mM)...
August 30, 2017: Cellular and Molecular Biology
keyword
keyword
118378
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"