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https://www.readbyqxmd.com/read/28806493/olaparib-a-clinically-used-poly-adp-ribose-polymerase-inhibitor-protects-against-oxidant-induced-cardiac-myocyte-death-in-vitro-and-improves-cardiac-contractility-during-early-phase-after-heart-transplantation-in-a-rat-model-in-vivo
#1
Sevil Korkmaz-Icöz, Bartosz Szczesny, Michela Marcatti, Shiliang Li, Mihály Ruppert, Felix Lasitschka, Sivakkanan Loganathan, Csaba Szabo, Gábor Szabó
BACKGROUND AND PURPOSE: Olaparib, rucaparib and niraparib, potent inhibitors of poly(ADP-ribose) polymerase (PARP) have recently been approved for human use for oncological indications. Considering the previously demonstrated role of PARP in various forms of acute and chronic myocardial injury, we tested the effect of olaparib in in-vitro models of oxidative stress in cardiomyocytes, and in an in-vivo model of cardiac transplantation. EXPERIMENTAL APPROACH: H9c2-embryonic rat heart-derived myoblasts pretreated with vehicle or olaparib (10μM) were challenged with either hydrogen-peroxide (H2 O2 ) or with glucose-oxidase (GOx, which generates H2 O2 in the tissue culture medium)...
August 14, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28800914/aneuploidy-police-detect-chromosomal-imbalance-triggering-immune-crackdown
#2
Emma V Watson, Stephen J Elledge
Aneuploidy is ubiquitous in cancer and plays a pivotal, early role in tumor evolution. It must therefore be avoided, and two recent papers highlight the roles of p53, senescence, and the immune system in preventing the outgrowth of aneuploid clones in tissue culture. These mechanisms are likely to synergize to maintain diploid cell populations.
August 8, 2017: Trends in Genetics: TIG
https://www.readbyqxmd.com/read/28800499/effects-of-preimplantation-factor-on-interleukin-6-and-prostaglandin-f2%C3%AE-and-e2-in-the-bovine-endometrium
#3
Ruth E Wonfor, Manuela Natoli, Michael T Rose, Deborah M Nash
Preimplantation factor (PIF) is a pregnancy specific peptide with immune modulatory properties exerted on the human endometrium. Viable bovine embryos secrete PIF, but its effect on the bovine endometrial immune response is unknown, both in native and inflammatory stimulated endometrial tissue. An ex vivo bovine endometrial tissue culture model was used with lipopolysaccharide (LPS) as an inflammatory stimulant. The effect of synthetic PIF (sPIF) was assessed, in three separate experiments, on the secretion or mRNA expression of essential prostaglandins and cytokines...
August 2, 2017: Theriogenology
https://www.readbyqxmd.com/read/28800363/the-viability-of-lytic-bacteriophage-%C3%AE-d5-in-potato-associated-environments-and-its-effect-on-dickeya-solani-in-potato-solanum-tuberosum-l-plants
#4
Robert Czajkowski, Anna Smolarska, Zofia Ozymko
Dickeya solani is one of the most important pectinolytic phytopathogens responsible for high losses in potato, especially in seed potato production in Europe. Lytic bacteriophages can affect the structure of the host population and may influence spread, survival and virulence of the pathogen and in consequence, infection of the plant. In this study, we aimed to acquire information on the viability of the broad host lytic bacteriophage ΦD5 on potato, as well as to apprehend the specific effect of this bacteriophage on its host D...
2017: PloS One
https://www.readbyqxmd.com/read/28796799/differential-outcomes-of-zika-virus-infection-in-aedes-aegypti-orally-challenged-with-infectious-blood-meals-and-infectious-protein-meals
#5
Yan-Jang S Huang, Amy C Lyons, Wei-Wen Hsu, So Lee Park, Stephen Higgs, Dana L Vanlandingham
BACKGROUND: Infection of mosquitoes is an essential step for the transmission of mosquito-borne arboviruses in nature. Engorgement of infectious blood meals from viremic infected vertebrate hosts allows the entry of viruses and initiates infection of midgut epithelial cells. Historically, the infection process of arboviruses in mosquitoes has been studied through the engorgement of mosquitoes from viremic laboratory animals or from artificial feeders containing blood mixed with viruses harvested from cell cultures...
2017: PloS One
https://www.readbyqxmd.com/read/28783560/a-study-of-extracellular-matrix-remodeling-in-aortic-heart-valves-using-a-novel-biaxial-stretch-bioreactor
#6
Ying Lei, Shirin Masjedi, Zannatul Ferdous
In aortic valves, biaxial cyclic stretch is known to modulate cell differentiation, extracellular matrix (ECM) synthesis and organization. We designed a novel bioreactor that can apply independent and precise stretch along radial and circumferential directions in a tissue culture environment. While this bioreactor can be used for either native or engineered tissues, this study determined matrix remodeling and strain distribution of aortic cusps after culturing under biaxial stretch for 14 days. The contents of collagen and glycosaminoglycans were determined using standard biochemical assays and compared with fresh controls...
July 27, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28782183/pectin-nanocoating-reduces-proinflammatory-fibroblast-response-to-bacteria
#7
A Mieszkowska, J Folkert, T Gaber, K Miksch, K Gurzawska
Implant failures are primarily related to bacterial infections and inflammation. Nanocoating of implant devices with organic molecules is a method used for improving their integration into host tissues and limiting inflammation. Bioengineered plant-derived rhamnogalacturonan-Is (RG-Is) from pectins improve tissue regeneration and exhibit anti-inflammatory properties. Therefore, the aim of this study is to evaluate the in vitro effect of RG-I nanocoating on human gingival primary fibroblast (HGF) activity and proinflammatory response following Porphyromonas gingivalis (P...
August 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28775044/senolytic-drugs-target%C3%A2-alveolar-epithelial-cell-function-and-attenuate-experimental-lung-fibrosis-ex-vivo
#8
Mareike Lehmann, Martina Korfei, Kathrin Mutze, Stephan Klee, Wioletta Skronska-Wasek, Hani N Alsafadi, Chiharu Ota, Rita Costa, Herbert B Schiller, Michael Lindner, Darcy E Wagner, Andreas Günther, Melanie Königshoff
Idiopathic pulmonary fibrosis (IPF) is a devastating lung disease with poor prognosis and limited therapeutic options. The incidence of IPF increases with age, and ageing-related mechanisms such as cellular senescence have been proposed as pathogenic drivers. The lung alveolar epithelium represents a major site of tissue injury in IPF and senescence of this cell population is probably detrimental to lung repair. However, the potential pathomechanisms of alveolar epithelial cell senescence and the impact of senolytic drugs on senescent lung cells and fibrosis remain unknown...
August 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28774806/preparation-of-new-natural-silk-non-woven-fabrics-by-using-adhesion-characteristics-of-sericin-and-their-characterization
#9
Ji Hye Lee, Yeon Su Bae, Su Jin Kim, Dae Woong Song, Young Hwan Park, Do Gyu Bae, Jin Hyun Choi, In Chul Um
Electro-spun regenerated silk webs have been extensively studied for biomedical applications because of the simplicity of their fabrication methods However, the productivity of the electro-spinning process is low for web fabrication and the mechanical properties of the electro-spun silk web are not satisfactory, which restricts its commercialization. In this study, a new silk non-woven fabric was successfully fabricated by wetting and hot press treatments using the excellent binding characteristic of sericin...
July 31, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28772708/nurse-s-a-phase-material-enhance-adhesion-growth-and-differentiation-of-human-bone-marrow-derived-stromal-mesenchymal-stem-cells
#10
Ruben Rabadan-Ros, Salvador Aznar-Cervantes, Patricia Mazón, Patricia Ros-Tarraga, Piedad N De Aza, Luis Meseguer-Olmo
The purpose of this study was to evaluate the bioactivity and cell response of a well-characterized Nurse's A-phase (7CaO·P₂O₅·2SiO₂) ceramic and its effect compared to a control (tissue culture polystyrene-TCPS) on the adhesion, viability, proliferation, and osteogenic differentiation of ahMSCs in vitro. Cell proliferation (Alamar Blue Assay), Alizarin Red-S (AR-s) staining, alkaline phosphatase (ALP) activity, osteocalcin (OCN), and collagen I (Col I) were evaluated. Also, field emission scanning electron microscopy (FESEM) images were acquired in order to visualise the cells and the topography of the material...
March 27, 2017: Materials
https://www.readbyqxmd.com/read/28771232/di-2-ethylhexyl-phthalate-exposure-impairs-meiotic-progression-and-dna-damage-repair-in-fetal-mouse-oocytes-in-vitro
#11
Jing-Cai Liu, Fang-Nong Lai, Ling Li, Xiao-Feng Sun, Shun-Feng Cheng, Wei Ge, Yu-Feng Wang, Lan Li, Xi-Feng Zhang, Massimo De Felici, Paul W Dyce, Wei Shen
Di (2-ethylhexyl) phthalate (DEHP), is the most common member of the class of phthalates that are used as plasticizers and have become common environmental contaminants. A number of studies have shown that DEHP exposure impacts reproductive health in both male and female mammals by acting as an estrogen analog. Here, we investigated the effects of DEHP on meiotic progression of fetal mouse oocytes by using an in vitro model of ovarian tissue culture. The results showed that 10 or 100 μM DEHP exposure inhibited the progression of oocytes throughout meiotic prophase I, specifically from the pachytene to diplotene stages...
August 3, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28770916/fluorine-substitution-enhances-the-self-assembling-ability-of-hydrogelators
#12
Chengfan Wu, Zhen Zheng, Yuenan Guo, Chongli Tian, Qiang Xue, Gaolin Liang
When supramolecular hydrogels are applied as tissue culture scaffolds, their mechanical strength and biocompatibility are the two most important factors that must be considered. However, systematic studies on the structure-mechanical property (or structure-cytotoxicity) relationship of hydrogels are rare. Herein, we rationally designed three hydrogelators and their corresponding phosphate precursors, and systematically studied their self-assembling ability and cytotoxicity. The results indicated that fluorine substitution, but not trifluoromethyl substitution with more fluorine atoms, to the phenylalanine motif enhanced the self-assembling ability and cytotoxicity of the hydrogelators (or precursors)...
August 3, 2017: Nanoscale
https://www.readbyqxmd.com/read/28762415/biocompatible-3d-printed-polymers-via-fused-deposition-modelling-direct-c2c12-cellular-phenotype-in-vitro
#13
Rowan P Rimington, Andrew J Capel, Steven D R Christie, Mark P Lewis
The capability to 3D print bespoke biologically receptive parts within short time periods has driven the growing prevalence of additive manufacture (AM) technology within biological settings, however limited research concerning cellular interaction with 3D printed polymers has been undertaken. In this work, we used skeletal muscle C2C12 cell line in order to ascertain critical evidence of cellular behaviour in response to multiple bio-receptive candidate polymers; polylactic acid (PLA), acrylonitrile butadiene styrene (ABS), polyethylene terephthalate (PET) and polycarbonate (PC) 3D printed via fused deposition modelling (FDM)...
August 1, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28761842/cutaneous-squamous-cell-carcinoma-in-lupus-vulgaris-caused-by-drug-resistant-mycobacterium-tuberculosis
#14
Muthu S Kumaran, Tarun Narang, Madhukara Jitendriya, Rajalakshmi Tirumale, Suraj Manjunath, Jayanthi Savio
Tuberculosis (TB) is still a major public health problem in the world, with many factors contributing to this burden, including poor living conditions, overcrowding, poverty, malnutrition, illiteracy, and rapid spread of human immunodeficiency virus infection. Cutaneous tuberculosis is a less common form of extrapulmonary tuberculosis, and in this paucibacillary form the diagnosis depends on histopathology, tuberculin positivity, and response to treatment. The diagnosis is even more difficult in cases with drug resistant Mycobacterium tuberculosis due to lack of awareness and lack of facilities to diagnose drug resistant tuberculosis...
July 2017: Indian Dermatology Online Journal
https://www.readbyqxmd.com/read/28755949/short-communication-passive-shedding-of-mycobacterium-avium-ssp-paratuberculosis-in-commercial-dairy-goats-in-brazil
#15
D G G Schwarz, M C Lima, M Barros, F L Valente, T M Scatamburlo, N Rosado, C T S A M Oliveira, L L Oliveira, M A S Moreira
Goat farming is a low-cost alternative to dairy production in developing countries. In Brazil, goat production has increased in recent years due in part to the implementation of programs encouraging this activity. Mycobacterium avium ssp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a disease that causes chronic granulomatous enteritis in ruminants, but MAP transmission dynamics are still poorly understood in goats. In a previously published study of our research group, 10 dairy goat farms (467 animals) from Minas Gerais state were analyzed for MAP detection; 2 fecal cultures and 11 milk samples tested positive for MAP by conventional PCR and were confirmed by sequencing...
July 26, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28755344/identifying-and-controlling-contamination-of-date-palm-tissue-cultures
#16
Abeer H I Abdel-Karim
Fungal and bacterial contaminations are major problems facing in vitro date palm (Phoenix dactylifera L.) proliferation. To overcome this problem, we must first identify the fungal (e.g., Alternaria sp., Aspergillus niger, Penicillium sp.) and bacterial (e.g., Pseudomonas sp.) spread in date palm in vitro cultures. Incorporating fungicides (e.g., copper oxychloride, Vitavax T, and Topsin M) or antibiotics (e.g., streptomycin, Banocin, and Bencid D) at 500 mg/L in medium significantly reduces the contamination rate during various stages of in vitro date palm culture...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28755336/enhanced-indirect-somatic-embryogenesis-of-date-palm-using-low-levels-of-seawater
#17
Rania A Taha
Date palm tolerates salinity, drought, and high temperatures. Arid and semiarid zones, especially the Middle East region, need a huge number of date palms for cultivation. To meet this demand, tissue culture techniques have great potential for mass production of plantlets, especially using the indirect embryogenesis technique; any improvement of these techniques is a worthy objective. Low levels of salinity can enhance growth and development of tolerant plants. A low level of seawater, a natural source of salinity, reduces the time required for micropropagation processes of date palm cv...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28755333/direct-organogenesis-from-immature-female-inflorescence-of-date-palm-by-gradual-reduction-of-2-4-d-concentration
#18
Ezz El-Din G Gadalla
Inflorescences represent an alternative explant source for superior date palm trees, especially those that do not produce offshoots. They provide large numbers of explants free of fungal and bacterial contamination for successful tissue culture initiation. Furthermore, they are characterized by the capacity of plant regeneration within a short time as compared to other explant types. This chapter focuses on the procedures employed for plant regeneration by direct organogenesis using immature female inflorescence explants, including initiation of adventitious buds, differentiation, multiplication, shoot elongation, rooting, and acclimatization...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28755217/molecular-identification-of-fungal-contamination-in-date-palm-tissue-cultures
#19
Mohammed H Abass
Fungal contamination of in vitro cultures of date palm (Phoenix dactylifera L.) is the major constraint to their initiation and maintenance. Different molecular approaches have been applied successfully to analyze both inter- and intraspecific variation among fungal species as well as determine their identity. This chapter describes step-by-step procedures of molecular identification of fungal contaminants by internal transcribed spacer (ITS) products of the most common fungal contaminants of date palm tissue culture...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28755216/evaluation-of-clonal-fidelity-of-micropropagated-date-palm-by-random-amplified-polymorphic-dna-rapd
#20
Arpan Modi, Bhavesh Gajera, Naraynan Subhash, Nitish Kumar
Date palm is a fruit-bearing tree commonly found in arid and semiarid regions. It is a dioecious plant, producing fruit on female plants and a limited number of basal offshoots for propagation. To produce large numbers of uniform plantlets, tissue culture techniques are required. It is highly advisable to detect genetic variation that may occur through micropropagation techniques as it may lead to phenotypic alterations. Random amplified polymorphic DNA (RAPD) is a simple and PCR-based molecular marker technique which can be employed to check the somaclonal variation...
2017: Methods in Molecular Biology
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