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https://www.readbyqxmd.com/read/29778472/effects-of-ad-libitum-milk-replacer-feeding-and-butyrate-supplementation-on-behavior-immune-status-and-health-of-holstein-calves-in-the-postnatal-period
#1
C Gerbert, D Frieten, C Koch, G Dusel, K Eder, T Stefaniak, J Bajzert, P Jawor, A Tuchscherer, H M Hammon
Animal welfare in dairy calf husbandry depends on calf rearing and is probably improved by intensive milk feeding programs. In addition, butyrate supplementation in milk replacer (MR) stimulates postnatal growth and may affect the immune system in calves. We have investigated the combined effects of ad libitum MR feeding and butyrate supplementation on feeding behavior, health, and the immune responses in calves. Holstein calves (n = 64) were examined from birth until wk 11 of age. Calves received MR either ad libitum (Adl) or restrictively (Res) with (AdlB+, ResB+) or without (AdlB-, ResB-) 0...
May 16, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29773143/sex-and-vaccination-insights-from-female-rats-vaccinated-with-juvenile-specific-proteases-from-fasciola-hepatica
#2
Agnieszka Wesołowska, Katarzyna Basałaj, Luke J Norbury, Alicja Sielicka, Halina Wędrychowicz, Anna Zawistowska-Deniziak
Most animal research is less evidence-based for females, with the majority of studies conducted on males. Since immune responses vary between males and females, sexual dimorphism in immunity contributes, among other things, to sex-based differences post-vaccination. However, the issue of sex effects in animal vaccine research is rarely considered in vaccine study design. Previously, we have evaluated the efficacy of cathepsin L3 (FhCL3-1 and FhCL3-2) and B3 proteases (FhCB3) from juvenile Fasciola hepatica as vaccines against fasciolosis in male rats...
May 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29773139/systemic-humoral-immunity-in-beef-bulls-following-therapeutic-vaccination-against-tritrichomonas-foetus
#3
Christopher Alling, D Owen Rae, Xiaojie Ma, Laura Neumann, L Gene Lollis, Elizabeth Steele, John Yelvington, Hemant K Naikare, Heather Stockdale Walden, John Crews, Raoul Boughton
The utility of therapeutic vaccination of bulls against Tritrichomonas foetus has been advocated in previous studies, but anecdotal reports suggest this practice does not clear infections and may additionally confound diagnostic testing by reducing parasite burdens below detectable limits. The objective of this study was to characterize the systemic humoral immune response to therapeutic vaccination in T. foetus-infected bulls over a period of four months using an indirect ELISA and to compare the dynamics of this response to culture and PCR results to establish the existence of a relationship (or lack thereof) between immunization and infection status...
May 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29769385/phase-ii-study-of-irinotecan-plus-panitumumab-as-second-line-therapy-for-patients-with-advanced-esophageal-adenocarcinoma
#4
Harry Yoon, Anita Choudhary, Ramla Kosozi, Gurvinder Singh Bali, Ali Zaidi, Ajlan Atasoy, Arlene A Forastiere, Michael K Gibson
LESSON LEARNED: Panitumumab plus irinotecan is not active for the treatment of esophageal adenocarcinoma. BACKGROUND: Esophageal adenocarcinoma (EAC) is a lethal cancer with increasing incidence. Panitumumab (Pa) is a fully humanized IgG2 monoclonal antibody against human EGFR. Cetuximab (Cx) combined with irinotecan (Ir) is active for second-line treatment of colorectal cancer. This phase II study was designed to evaluate Pa plus Ir as second-line therapy for advanced EAC...
May 16, 2018: Oncologist
https://www.readbyqxmd.com/read/29766466/concurrent-isolated-igg2-positive-membranous-nephropathy-and-malignant-b-cell-lymphoma
#5
Satoshi Shimada, Takashi Nakamichi, Gen Yamada, Kaori Narumi, Hajime Usubuchi, Tae Yamamoto, Satoshi Ichikawa, Noriko Fukuhara, Mariko Miyazaki, Hideo Harigae, Hiroshi Sato, Sadayoshi Ito
A recent systematic review showed that hematological malignancy is often complicated by membranous nephropathy (MN). Histologically, the deposition of IgG subclasses other than IgG4 may imply secondary MN, such as malignancy-associated MN (M-MN). We describe a very rare case of concurrent isolated IgG2-positive MN and B-cell lymphoma. An 83-year-old woman was hospitalized at our institute for facial and lower extremity edema persisting for 2 months. Laboratory tests showed urinary protein level of 10.8 g/day, serum albumin level of 1...
May 15, 2018: CEN Case Reports
https://www.readbyqxmd.com/read/29739081/subclasses-of-monoclonal-type-i-immunoglobulin-g-cryoglobulins-report-on-two-distinct-cases-with-myeloma
#6
Tania Petersen, Sebastien Riviere, Stephanie Malbos, Yannick Chantran, Aicha Abbas, Francois Chasset, Arsene Mekinian, Jeremie Sellam, Laurent Garderet, Pierre Aucouturier
BACKGROUND: While different clinical manifestations of IgM and IgG monoclonal cryoglobulins have been demonstrated, little is known about the roles of IgG subclasses in the pathophysiology of these conditions. METHODS: In two cases of myeloma-associated monoclonal (type I) cryoglobulinemia with quite distinct clinical and biological features, serum samples were analyzed using an original IgG subclass-specific immunoblotting technique. RESULTS: The first case had painful arthritis of hands and feet, with skin purpura and a sharp decrease of complement C4 level, and the cryoglobulin was of IgG1 subclass...
April 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/29731233/conformational-plasticity-of-the-immunoglobulin-fc-domain-in-solution
#7
Soumya G Remesh, Anthony A Armstrong, Andrew D Mahan, Jinquan Luo, Michal Hammel
Fragment crystallizable (Fc) region of immunoglobulin G (IgG) antibody binds to specific Fc receptors (FcγRs) to control antibody effector functions. Currently, engineered specific Fc-FcγR interactions are validated with a static conformation derived from the crystal structure. However, computational evidence suggests that the conformational variability of Fcs plays an important role in receptor recognition. Here we elucidate Fc flexibility of IgG1, IgG2, and IgG1 Fc with mutations (M255Y/S257T/T259E) in solution by small-angle X-ray scattering (SAXS)...
April 18, 2018: Structure
https://www.readbyqxmd.com/read/29731066/distribution-of-anti-abo-immunoglobulin-g-subclass-and-c1q-antibody-in-abo-incompatible-kidney-transplantation
#8
K H Lee, D I Won, J-M Yook, K Y Kim, S M Park, J H Park, E S Lee, J-H Lim, H-Y Jung, J-Y Choi, S-H Park, C-D Kim, Y-L Kim, H-K Kim, S Huh, J-H Cho
INTRODUCTION: To investigate the correlation between serum anti-ABO immunoglobulin G (IgG) and IgG subclasses, anti-ABO IgG subclasses were measured by flow cytometry (FCM) in ABO-incompatible (ABOi) kidney transplant recipients. We also evaluated baseline anti-ABO C1q antibody. METHOD: Baseline anti-ABO IgG titers were measured by both FCM and column agglutination technique methods in 18 ABOi kidney transplant recipients. The mean florescence intensity (MFI) ratios of baseline anti-ABO IgG subclasses and anti-ABO C1q antibody were obtained by FCM and followed-up after rituximab treatment, each plasmapheresis (PP) session, and kidney transplantation...
May 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29721239/meningitis-spondylodiscitis-pneumonia-and-septic-shock-with-streptococcus-pneumoniae-in-a-previously-healthy-woman-with-isolated-igg2-igg3-iga-deficiency-and-monoclonal-gammopathy-of-undetermined-significance
#9
Shahin Gaini, David Gudnason, Bjarni Á Steig, Jenny Jónsdóttir Nielsen
A 66 years old Caucasian woman with pneumococcal meningitis was treated and discharged after an uncomplicated course. Five months later she was readmitted with fever and right side abdominal pain and diagnosed with pneumococcal spondylodiscitis. One year later she was treated for a severe chest X-ray confirmed left lobar pneumonia. Two years later she was diagnosed with a pneumococcal pneumonia in her left lung with septic shock. An immune deficiency screen revealed slightly reduced IgA levels, low IgG2 levels, low IgG3 levels and high IgG1 levels...
March 29, 2018: Infectious Disease Reports
https://www.readbyqxmd.com/read/29706814/proliferative-glomerulonephritis-with-monoclonal-immunoglobulin-deposits-of-lambda-chains
#10
Muhammad A Panezai, Pingchaun Zhang, Gates B Colbert
Increasingly, monoclonal gammopathies of renal significance (MGRS) are being described as unique, distinct disease states. We describe a type of MGRS with proliferative glomerular lesions with monoclonal immunoglobulin deposits of rarely reported IgG2 lambda chains.
April 2018: Proceedings of the Baylor University Medical Center
https://www.readbyqxmd.com/read/29704938/evaluation-of-the-humoral-immune-response-to-a-multicomponent-recombinant-vaccine-against-s-aureus-in-healthy-pregnant-heifers
#11
N Pujato, C M Camussone, M S Renna, M S Perrig, B Morein, L F Calvinho, I S Marcipar
Staphylococcus aureus is a worldwide pathogen that causes mastitis in dairy herds. Shortcomings in control programs have encouraged the development of vaccines against this pathogen. This study evaluated the vaccine candidate VacR, which included recombinant S. aureus protein clumping factor A (rClf), fibronectin binding protein A (rFnBP) and hemolysin beta (rBt), formulated with a novel immune-stimulating complex. Comparisons were made between healthy pregnant heifers that received either VacR (n=8; VacR group) or phosphate buffered saline (PBS) plus adjuvant (control group) SC in the supramammary lymph node area on days 45 and 15 before the expected calving date...
May 2018: Veterinary Journal
https://www.readbyqxmd.com/read/29703767/encephalitis-with-mglur5-antibodies-symptoms-and-antibody-effects
#12
Marianna Spatola, Lidia Sabater, Jesús Planagumà, Eugenia Martínez-Hernandez, Thaís Armangué, Harald Prüss, Takahiro Iizuka, Ruben L Caparó Oblitas, Jean-Christophe Antoine, Richard Li, Nicholas Heaney, Niall Tubridy, Elvira Munteis Olivas, Myrna R Rosenfeld, Francesc Graus, Josep Dalmau
OBJECTIVE: To report the clinical features of 11 patients with metabotropic glutamate receptor 5 (mGluR5) antibody-associated encephalitis, immunoglobulin G (IgG) subclass, and effects of the antibodies on neuronal mGluR5 clusters. METHODS: Clinical information was retrospectively obtained from referring physicians. Antibodies to mGluR5 and IgG subclasses were determined with brain immunohistochemistry and cell-based assays. The effects of the antibodies were examined on rat hippocampal neurons with reported techniques...
April 27, 2018: Neurology
https://www.readbyqxmd.com/read/29693321/analytical-subcloning-of-a-clonal-cell-line-demonstrates-sequence-heterogeneity-that-does-not-impact-process-consistency-or-robustness
#13
John J Scarcelli, Megan Hone, Kathryn Beal, Alejaida Ortega, Bruno Figueroa, Jason A Starkey, Karin Anderson
During development of a cell line intended to support production of an IgG2 monoclonal antibody, a sequence variant caused by a genetic mutation was identified in the bulk drug substance. Gene copy number analysis together with the level of the observed variant in genomic DNA indicated that the master cell bank was a mixed population of cells; some harboring the variant copy and some mutation free. Since the cell bank had been single-cell cloned, this variant could be used as a biomarker to demonstrate either that the bank was not derived from a single cell, or that the variant was a result of a post-cloning genetic event, leading to a mixed population of cells...
April 25, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29684416/development-of-antibody-biomarkers-of-long-term-and-recent-dengue-virus-infections
#14
Eduardo J M Nascimento, James W Huleatt, Marli T Cordeiro, Priscila M S Castanha, James K George, Eduard Grebe, Alex Welte, Monique Brown, Donald S Burke, Ernesto T A Marques
Dengue virus (DENV) infections elicit antibody responses to the non-structural protein 1 (NS1) that are associated with protection against disease. However, the antibody isotypes and subclasses involved, and their kinetics have not been extensively studied. We characterized the antibody responses to DENV NS1 by enzyme-linked immunosorbent assay (ELISA) in a longitudinal cohort of 266 confirmed dengue cases in Recife, Northeast Brazil. Samples were collected during the febrile phase and up to over 3 years after onset of symptoms...
April 20, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29684046/intranasal-administration-of-a-two-dose-adjuvanted-multi-antigen-tmv-subunit-conjugate-vaccine-fully-protects-mice-against-francisella-tularensis-lvs-challenge
#15
Alison A McCormick, Aisha Shakeel, Chris Yi, Hardeep Kaur, Ahd M Mansour, Chandra Shekhar Bakshi
Tularemia is a fatal human disease caused by Francisella tularensis, a Gram-negative encapsulated coccobacillus bacterium. Due to its low infectious dose, ease of aerosolized transmission, and lethal effects, the CDC lists F. tularensis as a Category A pathogen, the highest level for a potential biothreat agent. Previous vaccine studies have been conducted with live attenuated, inactivated, and subunit vaccines, which have achieved partial or full protection from F. tularensis live vaccine strain (LVS) challenge, but no vaccine has been approved for human use...
2018: PloS One
https://www.readbyqxmd.com/read/29670279/safety-pharmacokinetics-and-biomarkers-of-f-652-a-recombinant-human-interleukin-22-dimer-in-healthy-subjects
#16
Kai-Yang Tang, Jason Lickliter, Zhi-Hua Huang, Zong-Shu Xian, Han-Yang Chen, Cheng Huang, Chong Xiao, Yu-Peng Wang, Ying Tan, Lin-Feng Xu, Yu-Liang Huang, Xiao-Qiang Yan
F-652 is a recombinant fusion protein consisting of two human interleukin-22 (IL-22) molecules linked to an immunoglobulin constant region (IgG2 -Fc). IL-22 plays critical roles in promoting tissue repair and suppressing bacterial infection. The safety, pharmacokinetics (PK), tolerability, and biomarkers of F-652 were evaluated following a single dose in healthy male volunteers in a randomized, double-blind, placebo-controlled study. Following single-dose subcutaneous (SC) injection of F-652 at 2.0 µg/kg into healthy subjects, six out of six subjects experienced delayed injection site reactions, which presented as erythematous and/or discoid eczematous lesions 10 to 17 days post-dosing...
April 18, 2018: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/29656998/changes-in-the-concentration-of-anti-leishmania-antibodies-in-saliva-of-dogs-with-clinical-leishmaniosis-after-short-term-treatment
#17
Ana Cantos-Barreda, Damián Escribano, José J Cerón, Fernando Tecles, Luis J Bernal, Silvia Martínez-Subiela
The aim of this study was to evaluate the possible changes in the concentration of anti-Leishmania antibodies in saliva samples from dogs with clinical leishmaniosis after short-term treatment. Twenty dogs with clinical signs and laboratory abnormalities compatible with canine leishmaniosis (CanL) were diagnosed and treated with a standard antimonial plus allopurinol therapy. The concentration of anti-Leishmania IgG2 and IgA antibodies in saliva was measured at the time of diagnosis (day 0) and after treatment (day 30) by time-resolved immunofluorometric assays (TR-IFMAs) and results were compared with those of serum...
April 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29625770/temperature-dependence-of-antibody-adsorption-in-protein-a-affinity-chromatography
#18
Walpurga Krepper, Peter Satzer, Beate Maria Beyer, Alois Jungbauer
Staphylococcal protein A affinity chromatography is a well-established platform for purification of clinical-grade antibodies. The wild type ligand has been mutated to improve caustic stability, elution behavior, and/or to increase binding capacity. Several modified protein A ligands are nowadays commercially available, one of them being the thermosensitive chromatography medium Byzen Pro from Nomadic Bioscience Co., Ltd. According to the manufacturer, Byzen Pro has the ability to release IgG upon a change in temperature...
March 30, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29625765/lema-and-erp-y-like-recombinant-proteins-from-leptospira-interrogans-protect-hamsters-from-challenge-using-addavax%C3%A2-as-adjuvant
#19
Thaís Larré Oliveira, Rodrigo Andrade Schuch, Guilherme Roig Inda, Bárbara Couto Roloff, Amilton Clair Pinto Seixas Neto, Marta Amaral, Odir Antonio Dellagostin, Daiane Drawanz Hartwig
BACKGROUND: Recombinant subunit vaccines have been extensively evaluated as promising alternatives against leptospirosis. Here, we evaluated two proteins in formulations containing the adjuvant AddaVax™ as vaccine candidates for prevention and control of leptospirosis. METHODS: Recombinant proteins rErp Y-like and rLemA were characterized by ELISA to assess their ability to bind extracellular matrix (ECM) components and fibrinogen. Groups of eight hamsters were immunized intramuscularly with rErp Y-like or rLemA mixed with a squalene-based adjuvant (AddaVax), and then vaccine efficacy was determined in terms of protection against a lethal challenge...
April 3, 2018: Vaccine
https://www.readbyqxmd.com/read/29618330/filaria-specific-antibody-response-profiling-in-plasma-from-anti-retroviral-na%C3%A3-ve-loa-loa-microfilaraemic-hiv-1-infected-people
#20
Ghislain Donald Njambe Priso, Abel Lissom, Loveline N Ngu, Nadesh N Nji, Jules Colince Tchadji, Thibau Flaurant Tchouangueu, Georgia E Ambada, Carole Stéphanie Sake Ngane, Brigitte Laure Dafeu, Larissa Djukouo, Inès Nyebe, Suzanne Magagoum, Apeh Alfred Ngoh, Ouambo Fotso Herve, Rosario Garcia, Anna Gutiérrez, Arinze S Okoli, Charles O Esimone, Flobert Njiokou, Chae Gyu Park, Alain Bopda Waffo, Godwin W Nchinda
BACKGROUND: In West and Central Africa areas of endemic Loa loa infections overlap with regions of high prevalence of human immunodeficiency virus type 1 (HIV-1) infections. Because individuals in this region are exposed to filarial parasites from birth, most HIV-1 infected individuals invariably also have a history of filarial parasite infection. Since HIV-1 infection both depletes immune system and maintains it in perpetual inflammation, this can hamper Loa loa filarial parasite mediated immune modulation, leading to enhanced loaisis...
April 4, 2018: BMC Infectious Diseases
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