keyword
MENU ▼
Read by QxMD icon Read
search

IgG2

keyword
https://www.readbyqxmd.com/read/28219072/human-allergen-specific-igg-subclass-antibodies-measured-using-immunocap-technology
#1
Robert Movérare, Karin Blume, Peter Lind, René Crevel, Åsa Marknell DeWitt, Stella Cochrane
BACKGROUND: Knowledge of human IgG subclass antibody responses to various allergens has been hampered by a lack of reliable standardized assays. The aim here was to develop quantitative immunoassays for human IgG1, IgG2, and IgG3 antibodies using ImmunoCAP® technology and to evaluate their application. METHODS: Enzyme conjugates with isotype-specific monoclonal antibodies and calibrators composed of purified myeloma paraproteins were developed for each assay and used together with other standardized assay reagents for the Phadia® 100 instrument...
February 21, 2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/28206982/biocompatible-chitosan-nanoparticles-as-an-efficient-delivery-vehicle-for-mycobacterium-tuberculosis-lipids-to-induce-potent-cytokines-and-antibody-response-through-activation-of-%C3%AE-%C3%AE-t-cells-in-mice
#2
Ishani Das, Avinash Padhi, Sitabja Mukherjee, Debi Dash, Santosh Kar, Avinash Sonawane
Activation of cell mediated and humoral immune responses to Mycobacterium tuberculosis (Mtb) are critical for protection and that nanoparticles mediated delivery of antigens are more potent in inducing different immune responses. Herein, we show that mice immunized with Mtb lipid bound chitosan nanoparticles(NPs) induce secretion of prominent type 1 T helper (Th1) and type 2 T helper (Th2) cytokines in lymph node and spleen cells, and also induced significantly higher levels of IgG, IgG1, IgG2 and IgM in comparison to control mice...
February 16, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28192236/diagnosis-of-primary-antibody-and-complement-immunodeficiencies-in-young-adults-after-a-first-invasive-bacterial-infection
#3
Sébastien Sanges, Frédéric Wallet, Nicolas Blondiaux, Didier Theis, Isabelle Verin, Anne Vachée, Rodrigue Dessein, Karine Faure, Nathalie Viget, Eric Senneville, Olivier Leroy, Fleur Maury, Nicolas Just, Julien Poissy, Daniel Mathieu, Anne Prévotat, Cécile Chenivesse, Arnaud Scherpereel, Grégoire Smith, Benjamin Lopez, Jérémie Rosain, Véronique Fremeaux-Bacchi, Eric Hachulla, Pierre-Yves Hatron, Mathilde Bahuaud, Frédéric Batteux, David Launay, Myriam Labalette, Guillaume Lefèvre
OBJECTIVES: Screening for primary immunodeficiencies (PIDs) in adults is recommended after two severe bacterial infections. We aimed to evaluate if screening should be performed after the first invasive infection in young adults. METHODS: Eligible patients were retrospectively identified using hospital discharge and bacteriology databases in 3 centers during a 3-year period. Eighteen to forty year-old patients were included if they had experienced an invasive infection with encapsulated bacteria commonly encountered in PIDs (Streptococcus pneumoniae (SP), Neisseria meningitidis (NM), Neisseria gonorrhoeae (NG), Haemophilus influenzae (HI) or group A Streptococcus (GAS))...
February 10, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28186329/effects-of-antibody-disulfide-bond-reduction-on-purification-process-performance-and-final-drug-substance-stability
#4
Wai Keen Chung, Brian Russell, Yanhong Yang, Michael Handlogten, Suzanne Hudak, Mingyan Cao, Jihong Wang, David Robbins, Sanjeev Ahuja, Min Zhu
Antibody disulfide bond reduction during monoclonal antibody (mAb) production is a phenomenon that has been attributed to the reducing enzymes from CHO cells acting on the mAb during the harvest process. However, the impact of antibody reduction on the downstream purification process has not been studied. During the production of an IgG2 mAb, antibody reduction was observed in the harvested cell culture fluid (HCCF), resulting in high fragment levels. In addition, aggregate levels increased during the low pH treatment step in the purification process...
February 10, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28177177/purification-and-quantification-of-heavy-chain-antibodies-from-the-milk-of-bactrian-camels
#5
Hongqiang Yao, Min Zhang, Yi Li, Jirimutu Yao, He Meng, Siriguleng Yu
Camel milk has a unique composition with naturally occurring heavy-chain antibodies (HCAbs), which exert rehabilitating potencies in infection and immunity. To characterize HCAb in camel milk, immunoglobulin G (IgG) was isolated from the milk of Camelus bactrianus by a combination of affinity chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis to purify and size-fractionate protein A and protein G, which were further identified by Western blotting, and were quantified by bicinchoninic acid (BCA) and ELISA...
February 8, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28174258/kinetic-modeling-of-the-release-of-ethylene-oxide-from-sterilized-plastic-containers-and-its-interaction-with-monoclonal-antibodies
#6
Bryan Lei Yu, Jun Han, Matthew Hammond, Xuemei Wang, Qingchun Zhang, Andrew Clausen, Ronald Forster, Mingda Eu
: Ethylene oxide (ETO) is commonly used to sterilize plastic containers, but the effects of residual amounts left after sterilization on protein therapeutics are still not well understood. Here we focus primarily on the factors that influence concentrations of ETO migrating from ETO-treated plastic containers into aqueous solution. A study was designed to investigate the kinetics of this process at various temperatures, and the kinetic data could be fit with a model based on a combination of Fickean diffusion and first-order chemical reaction (to account for observed hydrolysis of ETO)...
January 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/28166930/polymeric-antigen-blsomp31-in-aluminium-hydroxide-induces-serum-bactericidal-and-opsonic-antibodies-against-brucella-canis-in-dogs
#7
Maria Clausse, Alejandra G Díaz, Romina P Pardo, Vanesa Zylberman, Fernando A Goldbaum, Silvia M Estein
Polymeric antigen BLSOmp31 is an immunogenic vaccine candidate that confers protection against Brucella canis in mice. In this preliminary study, the immunogenicity and safety of BLSOmp31 adsorbed to aluminum hydroxide gel (BLSOmp31-AH) were evaluated in Beagle dogs. In addition, the potential to elicit serum antibodies with complement-dependent bactericidal activity and/or to enhance phagocytosis by neutrophils were analyzed. Dogs were immunized three times with BLSOmp31-AH by subcutaneous route, followed by an annual booster...
February 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28160866/withania-somnifera-chemotype-nmitli-101r-significantly-increases-the-efficacy-of-antileishmanial-drugs-by-generating-strong-ifn-%C3%AE-and-il-12-mediated-immune-responses-in-leishmania-donovani-infected-hamsters
#8
Chandra Dev Pati Tripathi, Pramod Kumar Kushawaha, Rajender Singh Sangwan, Chitra Mandal, Shailja Misra-Bhattacharya, Anuradha Dube
BACKGROUND: Withania somnifera (L.) Dunal (Solanaceae), commonly known as Ashwagandha, is one of the most important medicinal plant in the traditional Indian medical systems. Pharmacological studies have established that root extracts of W. somnifera contain several bioactive constituents called withanolides. The plant has long been used for its several beneficial properties and recently as an immunomodulator. HYPOTHESIS/PURPOSE: A combination therapy including a potential and safe immunostimulant with lower doses of effective drug, which can reduce the parasitic burden and simultaneously can produce an enhancement of adaptive immunity, has proven to be significantly a more effective approach than immunotherapy or drug therapy alone...
January 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28128471/lack-of-fc-gamma-receptor-iiia-promotes-rather-than-suppresses-humoral-and-cellular-immune-responses-after-mucosal-or-parenteral-immunization-with-antigen-and-adjuvants
#9
Jia-Bin Sun, Jan Holmgren, Mark S Cragg, Zou Xiang
The Fcγ receptor IIIA (FcγRIIIA) has traditionally been known as a positive regulator of immune responses. Consistent with this, mice deficient in FcγRIIIA are protected from various inflammation-associated pathologies including several autoimmune diseases. In contrast to this accepted dogma, we show here that mice lacking FcγRIIIA developed increased rather than reduced both humoral and cellular immune responses to mucosal (sublingual) immunization with ovalbumin (OVA) given together with the strong mucosal adjuvant cholera toxin as well as to parenteral (subcutaneous) immunization with OVA in complete Freund's adjuvant...
January 27, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28126470/ataxia-telangiectasia-immunodeficiency-and-survival
#10
Nienke J H van Os, Anne F M Jansen, Marcel van Deuren, Asgeir Haraldsson, Nieke T M van Driel, Amos Etzioni, Michiel van der Flier, Charlotte A Haaxma, Tomohiro Morio, Amit Rawat, Michiel H D Schoenaker, Annarosa Soresina, Alexander M R Taylor, Bart P C van de Warrenburg, Corry M R Weemaes, Nel Roeleveld, Michèl A A P Willemsen
Ataxia-telangiectasia (AT) is a neurodegenerative disorder characterized by ataxia, telangiectasia, and immunodeficiency. An increased risk of malignancies and respiratory diseases dramatically reduce life expectancy. To better counsel families, develop individual follow-up programs, and select patients for therapeutic trials, more knowledge is needed on factors influencing survival. This retrospective cohort study of 61 AT patients shows that classical AT patients had a shorter survival than variant patients (HR 5...
January 23, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/28120234/ataxia-telangiectasia-in-turkey-multisystem-involvement-of-91-patients
#11
Hacer Akturk, Murat Sutcu, Ayper Somer, Sanem Piskin, Manolya Acar, Meral Ozmen, Umut Altinoglu, Burak Tatli, Nuran Salman
BACKGROUND: Ataxia telangiectasia (AT) is a genetically based multisystemic disorder. We aimed to make a comprehensive evaluation of multisystem involvement in AT by describing clinical features and outcome of 91 patients. METHODS: Medical records of the patients who were diagnosed and followed by a multidisciplinary approach during a 27-year period (1988-2015) were reviewed retrospectively. RESULTS: Forty six female and 45 male patients with a mean follow-up period of 39...
January 25, 2017: World Journal of Pediatrics: WJP
https://www.readbyqxmd.com/read/28118850/humans-from-wuchereria-bancrofti-endemic-area-elicit-substantial-immune-response-to-proteins-of-the-filarial-parasite-brugia-malayi-and-its-endosymbiont-wolbachia
#12
Ruchi Jha, Mamta Gangwar, Dhanvantri Chahar, Anand Setty Balakrishnan, Mahendra Pal Singh Negi, Shailja Misra-Bhattacharya
BACKGROUND: In the past, immune responses to several Brugia malayi immunodominant antigens have been characterized in filaria-infected populations; however, little is known regarding Wolbachia proteins. We earlier cloned and characterized few B. malayi (trehalose-6-phosphate phosphatase, Bm-TPP and heavy chain myosin, BmAF-Myo) and Wolbachia (translation initiation factor-1, Wol Tl IF-1 and NAD(+)-dependent DNA ligase, wBm-LigA) proteins and investigated the immune responses, which they triggered in animal models...
January 24, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28111238/age-specific-profiles-of-antibody-responses-against-respiratory-syncytial-virus-infection
#13
Nao Jounai, Megumi Yoshioka, Miyuki Tozuka, Kazue Inoue, Tatsuya Oka, Kazuki Miyaji, Katsuyasu Ishida, Naoki Kawai, Hideyuki Ikematsu, Chiaki Kawakami, Hiroyuki Shimizu, Masaaki Mori, Ken J Ishii, Fumihiko Takeshita
Respiratory syncytial virus (RSV) is one of the most prevalent causative agents of lower respiratory tract infections worldwide, especially in infants around 3 to 4months old. Infants at such a young age have maternally-transferred passive antibodies against RSV but do not have active immune systems efficient enough for the control of RSV infection. In order to elucidate age-specific profiles of immune responses against RSV protection, antibody responses were examined by using blood samples in both acute and convalescent phases obtained from child patients and adult patients...
January 16, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28091579/impact-of-age-and-vaccination-history-on-long-term-serological-responses-after-symptomatic-b-pertussis-infection-a-high-dimensional-data-analysis
#14
Inonge van Twillert, Axel A Bonačić Marinović, Betsy Kuipers, Jacqueline A M van Gaans-van den Brink, Elisabeth A M Sanders, Cécile A C M van Els
Capturing the complexity and waning patterns of co-occurring immunoglobulin (Ig) responses after clinical B. pertussis infection may help understand how the human host gradually loses protection against whooping cough. We applied bi-exponential modelling to characterise and compare B. pertussis specific serological dynamics in a comprehensive database of IgG, IgG subclass and IgA responses to Ptx, FHA, Prn, Fim2/3 and OMV antigens of (ex-) symptomatic pertussis cases across all age groups. The decay model revealed that antigen type and age group were major factors determining differences in levels and kinetics of Ig (sub) classes...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28090683/similar-but-not-consistent-revisiting-the-pitfalls-of-measuring-igg-subclasses-with-different-assays
#15
Beatrice Ludwig-Kraus, Frank Bernhard Kraus
BACKGROUND: Laboratory quantification of IgG subclasses (IgGSc) is a well-established second-line tool for differential diagnosis of immune deficiencies. However, so far there is still no internationally approved standard available for IgGSc, and different assays are prone to produce divergent results. In this study, we evaluated the comparability and equivalence of two commercially available IgGSc assays, one being the Siemens IgGSc assay on a BN ProSpec analyzer and the other being The Binding Site (TBS) IgGSc assay on a Roche cobas c502 analyzer...
January 16, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28078662/low-immunoglobulin-e-flags-two-distinct-types-of-immune-dysregulation
#16
M Elkuch, V Greiff, C T Berger, M Bouchenaki, T Daikeler, A Bircher, A A Navarini, I Heijnen, M Recher
During the last two decades, hyper-immunoglobulin (Ig)E syndromes have been characterized clinically and molecularly in patients with genetically determined primary immunodeficiencies. However, the detection of low IgE levels, defined here as below detection limit in the routine clinical immunology laboratory, has received little attention. We analysed the association of serum IgA, IgM and IgG levels (including IgG subclasses) with low, normal or high serum IgE levels in patients evaluated in a single-centre out-patient immunodeficiency and allergy clinic...
March 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28076511/passive-acquisition-of-anti-staphylococcus-aureus-antibodies-by-newborns-via-transplacental-transfer-and-breastfeeding-regardless-of-maternal-colonization
#17
Maria Isabel Valdomir Nadaf, Laila Lima, Inês Stranieri, Olga Akiko Takano, Magda Carneiro-Sampaio, Patricia Palmeira
OBJECTIVE: To investigate the transmission of anti-Staphylococcus aureus (Sa) IgG, IgG1 and IgG2 via placental transfer and the transfer of IgA via the colostrum according to maternal Sa carrier status at delivery. METHODS: We evaluated anti-Sa IgG, IgG1 and IgG2 in maternal and cord sera and IgA in colostrum from a case (n=49, Sa+) and a control group (n=98, Sa-). RESULTS: Of the 250 parturients analyzed for this study, 49 were nasally colonized with S...
December 1, 2016: Clinics
https://www.readbyqxmd.com/read/28075502/antibody-and-cytokine-levels-in-humans-fed-on-by-the-common-bed-bug-cimex-lectularius-l
#18
Johnathan M Sheele, Gale E Ridge, Katirina Coppolino, Tracey Bonfield, Andrew B Young, Stephanie L Gaines, Thomas S McCormick
Little is known about cimicosis, the resultant dermal reaction from feeding activity by the common bed bug, Cimex lectularius L. We fed C. lectularius on human study subjects four times over four weeks, measured serum cytokine and antibody levels, and subjects recorded any cimicosis. The average time for subjects to develop cimicosis decreased with each feeding from 8.4, to 2.1, 1.5, and 1.3 days, respectively. There were no significant changes in total IgG, IgG1, IgG2, IgG4, or IgE levels between the first and fourth bed bug feedings, but there was a significant decrease in total IgG3 levels (p < 0...
January 11, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28061786/attenuated-actinobacillus-pleuropneumoniae-double-deletion-mutant-s-8%C3%A2-clpp-apxiic-confers-protection-against-homologous-or-heterologous-strain-challenge
#19
RANDOMIZED CONTROLLED TRIAL
Fang Xie, Gang Li, Long Zhou, Yanhe Zhang, Ning Cui, Siguo Liu, Chunlai Wang
BACKGROUND: Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, which leads to large economic losses to the swine industry worldwide. In this study, S-8△clpP△apxIIC, a double-deletion mutant of A. pleuropneumoniae was constructed, and its safety and protective efficacy were evaluated in pigs. RESULTS: The S-8△clpP△apxIIC mutant exhibited attenuated virulence in a murine (BALB/c) model, and caused no detrimental effects on pigs even at a dose of up to 1...
January 6, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28018355/biophysical-and-functional-characterization-of-rhesus-macaque-igg-subclasses
#20
Austin W Boesch, Nana Yaw Osei-Owusu, Andrew R Crowley, Thach H Chu, Ying N Chan, Joshua A Weiner, Pranay Bharadwaj, Rufus Hards, Mark E Adamo, Scott A Gerber, Sarah L Cocklin, Joern E Schmitz, Adam R Miles, Joshua W Eckman, Aaron J Belli, Keith A Reimann, Margaret E Ackerman
Antibodies raised in Indian rhesus macaques [Macaca mulatta (MM)] in many preclinical vaccine studies are often evaluated in vitro for titer, antigen-recognition breadth, neutralization potency, and/or effector function, and in vivo for potential associations with protection. However, despite reliance on this key animal model in translation of promising candidate vaccines for evaluation in first in man studies, little is known about the properties of MM immunoglobulin G (IgG) subclasses and how they may compare to human IgG subclasses...
2016: Frontiers in Immunology
keyword
keyword
118321
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"