Ekaterina N Kochkina, Elizaveta E Kopylova, Olga A Rogachevskaja, Nina P Kovalenko, Natalia V Kabanova, Polina D Kotova, Marina F Bystrova, Stanislav S Kolesnikov
In mammals, three genes encode IP3 receptors (IP3 Rs), which are involved in agonist-induced Ca2+ signaling in cells of apparently all types. Using the CRISPR/Cas9 approach for disruption of two out of three IP3 R genes in HEK-293 cells, we generated three monoclonal cell lines, IP3R1-HEK, IP3R2-HEK, and IP3R3-HEK, with the single functional isoform, IP3 R1, IP3 R2, and IP3 R3, respectively. All engineered cells responded to ACh with Ca2+ transients in an "all-or-nothing" manner, suggesting that each IP3 R isotype was capable of mediating CICR...
March 22, 2024: Cells