Assim Verma, Ramesh Kumar Dedar, Ram Kumar, Yogesh Chander, Himanshu Kamboj, Garvit Kumar, Rekha Verma, Santosh Kumari, Shalini Sharma, Bhupendra N Tripathi, Sanjay Barua, Naveen Kumar
In this study, we demonstrated the antiviral efficacy of hesperetin against multiple poxviruses, including buffalopox virus (BPXV), vaccinia virus (VACV), and lumpy skin disease virus (LSDV). The time-of-addition and virus step-specific assays indicated that hesperetin reduces the levels of viral DNA, mRNA, and proteins in the target cells. Further, by immunoprecipitation (IP) of the viral RNA from BPXV-infected Vero cells and a cell-free RNA-IP assay, we demonstrated that hesperetin-induced reduction in BPXV protein synthesis is also consistent with diminished interaction between eukaryotic translation initiation factor eIF4E and the 5' cap of viral mRNA...
April 2024: Journal of Medical Virology