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https://www.readbyqxmd.com/read/27910904/melanosome-transfer-to-keratinocyte-in-the-chicken-embryonic-skin-is-mediated-by-vesicle-release-associated-with-rho-regulated-membrane-blebbing
#1
Ryosuke Tadokoro, Hidetaka Murai, Ken-Ichiro Sakai, Takahiro Okui, Yasuhiro Yokota, Yoshiko Takahashi
During skin pigmentation in amniotes, melanin synthesized in the melanocyte is transferred to keratinocytes by a particle called the melanosome. Previous studies, mostly using dissociated cultured cells, have proposed several different models that explain how the melanosome transfer is achieved. Here, using a technique that labels the plasma membrane of melanocytes within a three-dimensional system that mimics natural tissues, we have visualized the plasma membrane of melanocytes with EGFP in chicken embryonic skin...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910763/il10-a-tale-of-an-evolutionarily-conserved-cytokine-across-vertebrates
#2
M Carla Piazzon, George Lutfalla, Maria Forlenza
IL10 was discovered in 1989, and since then it has been the subject of intense investigation, which has revealed its potent anti-inflammatory and regulatory activities in most immune processes during infection and disease. In 2003, the first non-mammalian IL10 sequence was identified in teleost fish, followed in 2004 by the chicken IL10 sequence. In this review, we summarize the work performed in non-mammalian vertebrates in which the IL10, IL10 receptors (IL10Rs), and their signaling components have been identified...
2016: Critical Reviews in Immunology
https://www.readbyqxmd.com/read/27910748/factors-influencing-the-amino-acid-and-peptide-transporter-proteins-mrna-expression-in-avian-species
#3
Bahman Navidshad, Seyed Ziaodin Mirhoseini
The commercial strains of broiler chickens have genetically selected to reach market weight at younger ages. In order to sustain increased growth rate requires an efficient assimilation of nutrients from the intestinal lumen, which is permitted through nutrient assimilation through the enterocytes that is facilitated by many nutrient transporter proteins. These trans-membrane proteins have specific action, well known kinetics and assist in the partitioning of nutrients among tissues. Amino acids are transported across the plasma membrane as free amino acids or short chain peptides through several independent systems encoded by various nutrient transporter gene products, and these systems may act on multiple substrates...
2016: Critical Reviews in Eukaryotic Gene Expression
https://www.readbyqxmd.com/read/27909922/production-of-biologically-active-recombinant-goose-fsh-in-a-single-chain-form-with-a-ctp-linker-sequence
#4
Hui Li, Huanxi Zhu, Qinming Qin, Mingming Lei, Zhendan Shi
FSH is a glycoprotein hormone secreted by the pituitary gland that is essential for gonadal development and reproductive function. In avian reproduction study, especially in avian reproduction hormone study, it is hindered by the lack of biologically active FSH. In order to overcome this shortcoming, we prepared recombinant goose FSH as a single chain molecule and tested its biological activities in the present study. Coding sequences for mature peptides of goose FSH α and β subunits were amplified from goose pituitary cDNA...
December 1, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27908832/evaluation-of-two-strains-of-marek-s-disease-virus-serotype-1-for-the-development-of-recombinant-vaccines-against-very-virulent-infectious-bursal-disease-virus
#5
Kai Li, Yongzhen Liu, Changjun Liu, Li Gao, Yulong Gao, Yanping Zhang, Hongyu Cui, Xiaole Qi, Li Zhong, Xiaomei Wang
Attenuated strains of Marek's disease virus serotype 1 (MDV1), and the closely related herpesvirus of turkeys, are among the most potent vectors for development of recombinant vaccines for poultry. To investigate the effects of MDV1 strain characteristics on the protective efficacy of the recombinant vaccines, we developed two recombinant MDV1 vaccines for expressing the VP2 gene of infectious bursal disease virus (IBDV) based on two different MDV1 strains, the attenuated strain 814 and the Meq gene-deleted recombinant MDV1 strain rLMS△Meq, as the viral vectors...
November 28, 2016: Antiviral Research
https://www.readbyqxmd.com/read/27908427/the-influence-of-oral-copper-methionine-on-matrix-metalloproteinase-2-gene-expression-and-activation-in-right-sided-heart-failure-induced-by-cold-temperature-a-broiler-chicken-perspective
#6
Mina Bagheri Varzaneh, Hamidreza Rahmani, Rahman Jahanian, Amir Hossein Mahdavi, Corinne Perreau, Gwenn Perrot, Stéphane Brézillon, François-Xavier Maquart
This study was designed to investigate the expression, activation and activity of matrix metalloproteinase-2 (MMP-2) in the heart of broiler chickens reared in cold conditions and fed with copper-methionine supplement at different levels. The chickens (n=480) were randomly allotted to six treatments and four replicates. Treatments included two rearing temperatures (i.e. normal and cold temperatures) each combined with three levels of supplemental copper-methionine (i.e. 0, 100 and 200mg/kg). On d 38 and 45 of age, four broilers from each treatment were sacrificed and their hearts were stored at -80°C...
January 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/27907152/dietary-probiotic-bacillus-subtilis-strain-fmbj-increases-antioxidant-capacity-and-oxidative-stability-of-chicken-breast-meat-during-storage
#7
Wen Kai Bai, Fei Jing Zhang, Tian Jin He, Peng Wei Su, Xiong Zhi Ying, Li Li Zhang, Tian Wang
This study was aimed to measure the dietary effects of probiotic Bacillus subtilis strain fmbj (BS fmbj) on antioxidant capacity and oxidative stability of chicken breast meat during storage. Treatment groups were fed the basal diet with BS fmbj at 0 g/kg (CON), 0.2 g/kg (BS-1), 0.3 g/kg (BS-2), or 0.4 g/kg (BS-3) doses without antibiotics. During 8 days of storage at 4°C, BS-2 group showed a significant improvement (P < 0.05) on meat quality (pH, Drip loss, Cooking loss, Shear force, color L*, a*, b*), free radical scavenging activity (DPPH, ABTS+, H2O2), tissues antioxidant enzyme capacity (SOD, CAT, GSH-Px, GSH, T-SH), mitochondria antioxidant enzyme capacity (MnSOD, GPx, GSH), mRNA expression of antioxidant genes (Nrf2, HO-1, SOD, CAT, GSH-Px) and mitochondrial function genes (avUCP, NRF1, NRF2, TFAM, PGC-1α), oxidative damage index (MDA, ROS, PC, 8-OHdG), and MMP level in chicken breast meat as compared to the CON group...
2016: PloS One
https://www.readbyqxmd.com/read/27907066/b-cell-based-seamless-engineering-of-antibody-fc-domains
#8
Koji Hashimoto, Kohei Kurosawa, Akiho Murayama, Hidetaka Seo, Kunihiro Ohta
Engineering of monoclonal antibodies (mAbs) enables us to obtain mAbs with additional functions. In particular, modifications in antibody's Fc (fragment, crystallizable) region can provide multiple benefits such as added toxicity by drug conjugation, higher affinity to Fc receptors on immunocytes, or the addition of functional modules. However, the generation of recombinant antibodies requires multiple laborious bioengineering steps. We previously developed a technology that enables rapid in vitro screening and isolation of specific mAb-expressing cells from the libraries constructed with chicken B-cell line DT40 (referred to as the 'ADLib system')...
2016: PloS One
https://www.readbyqxmd.com/read/27906584/effects-of-the-transforming-growth-factor-beta-signaling-pathway-on-the-differentiation-of-chicken-embryonic-stem-cells-into-male-germ-cells
#9
Yani Zhang, Yingjie Wang, Qisheng Zuo, Dong Li, Wenhui Zhang, Chao Lian, Beibei Tang, Tianrong Xiao, Man Wang, Kehua Wang, Bichun Li
The objectives of the present study were to screen for key gene and signaling pathways involved in the production of male germ cells in poultry and to investigate the effects of the transforming growth factor beta (TGF-β) signaling pathway on the differentiation of chicken embryonic stem cells (ESCs) into male germ cells. The ESCs, primordial germ cells, and spermatogonial stem cells (SSCs) were sorted using flow cytometry for RNA sequencing (RNA-seq) technology. Male chicken ESCs were induced using 40 ng/mL of bone morphogenetic protein 4 (BMP4)...
November 2016: Cellular Reprogramming
https://www.readbyqxmd.com/read/27905252/preparing-registrants-for-mentor-roles-the-chicken-or-egg-conundrum
#10
Claire McGuinness, Jacqueline McCallum, Kathleen Duffy
The Nursing and Midwifery Council's (NMC) Standards to Support Learning and Assessment in Practice ( 2008 ) outline requirements for the preparation of those who support nursing and midwifery pre-registration students in practice, formally known as mentors. Pre-registration nursing and midwifery programme providers, and practice learning environments (PLEs), work collaboratively to prepare registrants to undertake this role, and to help them maintain mentor status. An important NMC requirement is that registrants, when undertaking mentor preparation programmes, must be supported by experienced mentors in their workplace...
December 1, 2016: Nursing Management (Harrow)
https://www.readbyqxmd.com/read/27904397/antibacterial-activity-of-indian-borage-plectranthus-amboinicus-benth-leaf-extracts-in-food-systems-and%C3%A2-against-natural-microflora-in-chicken-meat
#11
Sandeep Kumar Gupta, Pradeep Singh Negi
The ability of acetone and ethyl acetate extracts of the leaves of a traditional Indian medicinal plant, Indian borage (Plectranthus amboinicus Benth) to prevent spoilage of artificially inoculated model food systems (cabbage and papaya) and natural microflora of chicken meat was evaluated. These extracts were able to reduce the bacterial counts in all food systems; however, the effective concentration varied with the complexity of the system (cabbage<papaya<chicken). A probable mode of action of extracts was investigated by analyzing the changes they cause in bacterial cell wall and leakage of nucleic acid from bacterial cells...
March 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27903990/baicalein-reduces-angiogenesis-in-the-inflammatory-microenvironment-via-inhibiting-the-expression-of-ap-1
#12
Yujie Huang, Zhaorui Miao, Yang Hu, Yang Yuan, Yuxin Zhou, Libin Wei, Kai Zhao, Qinglong Guo, Na Lu
Increasing clinical and experimental studies have demonstrated that refractory chronic inflammation will result in malignant tumor and anti-angiogenic therapy may be an effective way to thwart the progression. Baicalein, one of the major active flavanoids found in Scutellaria baicalensis Georgi, has been exhibited potent anti-inflammation and anti-tumor effects by reducing angiogenesis. However, the exact mechanism of baicalein on endothelial cells in inflammatory microenvironment was not clear yet. Here, we investigated the anti-angiogenic effect of baicalein by incubating human umbilical vein endothelial cells (HUVECs) with THP-1 conditioned medium in vitro...
November 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27903700/dietary-fat-alters-the-response-of-hypothalamic-neuropeptide-y-to-subsequent-energy-intake-in-broiler-chickens
#13
Xiao J Wang, Shao H Xu, Lei Liu, Zhi G Song, Hong C Jiao, Hai Lin
Dietary fat affects appetite and appetite-related peptides in birds and mammals; however, the effect of dietary fat on appetite is still unclear in chickens faced with differential energy statuses. Two experiments were conducted to investigate the effects of dietary fat on feed intake and hypothalamic neuropeptides in chickens subjected to two feeding states or two diets. In Experiment 1, chickens were fed a high-fat diet (HF) or low-fat diet (LF) for 35d, and then subjected to fed (HF-fed, LF-fed) or fasted (HF-fasted, LF-fasted) statuses for 24h...
November 30, 2016: Journal of Experimental Biology
https://www.readbyqxmd.com/read/27903065/predominance-and-geo-mapping-of-avian-influenza-h5n1-in-poultry-sectors-in-egypt
#14
Abdelsatar Arafa, Ihab El-Masry, Shereen Khoulosy, Mohammed K Hassan, Moussa Soliman, Olubunmi G Fasanmi, Folorunso O Fasina, Gwenaelle Dauphin, Juan Lubroth, Yilma M Jobre
Highly pathogenic avian influenza (HPAI) virus of the H5N1 subtype has been enzootic in the Egyptian poultry with significant human infections since 2008. This work evaluates the epidemiological and virological information from February 2006 to May 2015 in spatial and temporal terms. Only data with confirmed HPAI H5N1 sub-type were collected, and matched with the epidemiological data from various spatially and temporally-dispersed surveillances implemented between 2006 and 2015. Spatio-temporal analysis was conducted on a total of 3338 confirmed H5N1 HPAI poultry disease outbreaks and outputs described based on transmission patterns, poultry species, production types affected, trade, geographic and temporal distributions in Egypt...
November 28, 2016: Geospatial Health
https://www.readbyqxmd.com/read/27902918/recovery-of-chicken-growth-plate-by-heat-shock-protein-90-inhibitors-epigallocatechin-3-gallate-and-apigenin-in-thiram-induced-tibial-dyschondroplasia
#15
Muhammad Kashif Iqbal, Jingying Liu, Fazul Nabi, Mujeeb Ur Rehman, Hui Zhang, Adnan Hassan Tahir, Jiakui Li
Tibial dyschondroplasia (TD) is an important tibiotarsal bone disorder characterized by an avascular and nonmineralized growth plate; it is attributed to abnormal differentiation of chondrocytes and causes lameness. Heat-shock protein 90 (Hsp90) is a proangiogenic factor in animal tissues; however, its gene expression increases in cases of chicken TD. The objective of this study was to evaluate the efficacy of epigallocatechin-3-gallate (EGCG) and apigenin in thiram-induced TD birds; these substances were used because of their Hsp90 inhibitory activities...
December 2016: Avian Diseases
https://www.readbyqxmd.com/read/27902915/characterization-of-chicken-splenic-derived-dendritic-cells-following-vaccine-and-very-virulent-strains-of-infectious-bursal-disease-virus-infection
#16
A R Yasmin, S K Yeap, M Hair-Bejo, A R Omar
Studies have shown that infectious bursal disease virus (IBDV) infects lymphoid cells, mainly B cells and macrophages. This study was aimed to examine the involvement of chicken splenic-derived dendritic cells (ch-sDCs) in specific-pathogen-free chickens following inoculation with IBDV vaccine strain (D78) and a very virulent (vv) strain (UPM0081). Following IBDV infection, enriched activated ch-sDCs were collected by using the negative selection method and were examined based on morphology and immunophenotyping to confirm the isolation method for dendritic cells (DCs)...
December 2016: Avian Diseases
https://www.readbyqxmd.com/read/27902914/inactivation-of-avian-influenza-virus-in-nonpelleted-chicken-feed
#17
H Toro, V L van Santen, C Breedlove
Corn stored outside could become contaminated with avian influenza virus (AIV) from wild bird droppings. AIV-contaminated ingredients could pass into the poultry flocks in nonpelleted chicken feed. The efficacy of two disinfectants at inactivating AIV in chicken feed was evaluated. Both Termin-8 (a blend of formaldehyde, propionic acid, terpenes, and surfactant) and Finio (a blend of approved phytochemicals and carboxylic acids) effectively inactivated AIV in chicken feed. Because stability of infectious AIV in chicken feed is limited, we evaluated addition of protein (skim milk powder) to the virus suspension...
December 2016: Avian Diseases
https://www.readbyqxmd.com/read/27902913/regulatory-considerations-for-the-approval-of-drugs-against-histomoniasis-blackhead-disease-in-turkeys-chickens-and-game-birds-in-the-united-states
#18
Prajwal R Regmi, Ashley L Shaw, Laura L Hungerford, Janis R Messenheimer, Tong Zhou, Padmakumar Pillai, Amy Omer, Jeffrey M Gilbert
Histomoniasis, commonly referred to as blackhead disease, is a serious threat to the turkey and game bird industries worldwide, and it is having an increasingly negative impact on the chicken industry as well. The Food and Drug Administration's (FDA) Center for Veterinary Medicine (CVM), charged with the approval and regulation of new animal drugs in the United States, understands the rising need for the availability of therapeutic options against histomoniasis. CVM has actively engaged in discussions with the poultry industry, academic institutions, and animal health companies regarding the current status of histomoniasis in the United States and varied success of past and current management, prophylactic, and therapeutic interventions that have been used against the disease...
December 2016: Avian Diseases
https://www.readbyqxmd.com/read/27902910/recombinant-hemagglutinin-of-avian-influenza-virus-h5-expressed-in-the-chloroplast-of-chlamydomonas-reinhardtii-and-evaluation-of-its-immunogenicity-in-chickens
#19
Inkar Castellanos-Huerta, Bernardo Bañuelos-Hernández, Guillermo Téllez, Sergio Rosales-Mendoza, Luis G Brieba, Elizabeth Esquivel-Ramos, Josué I Beltrán-López, Gilberto Velazquez, Isidro Fernandez-Siurob
Globally, avian influenza (AI) is a serious problem in poultry farming. Despite vaccination, the prevalence of AI in México highlights the need for new approaches to control AI and to reduce the economic losses associated with its occurrence in susceptible birds. Recombinant proteins from avian influenza virus (AIV) have been expressed in different organisms, such as plants. The present study investigated the feasibility of designing and expressing the HA protein of AIV in the transplastomic microalga Chlamydomonas reinhardtii as a novel approach for AIV control and taking advantage of culture conditions, its reproductive range, and safe use in consideration of the generally regarded as safe food ingredient regulatory classification...
December 2016: Avian Diseases
https://www.readbyqxmd.com/read/27902909/marek-s-disease-in-backyard-chickens-a-study-of-pathologic-findings-and-viral-loads-in-tumorous-and-nontumorous-birds
#20
Aslı Mete, Radhika Gharpure, Maurice E Pitesky, Dan Famini, Karen Sverlow, John Dunn
Marek's disease (MD) is a major cause of mortality in backyard chickens. The diagnosis of MD is complex, however, and knowledge of Marek's disease virus (MDV) in spontaneous field cases such as in backyard chickens is largely unknown. In this study, 40 backyard chickens with a presumptive MD diagnosis based on histologic lymphoid infiltrations in peripheral nerves with and without lymphomas were investigated. Twenty-eight of the birds were submitted to the diagnostic laboratory for disease explorations, and 12 chickens were from a flock in which some members demonstrated anisocoria and pupil irregularities compatible with ocular MD...
December 2016: Avian Diseases
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