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https://www.readbyqxmd.com/read/28943908/expression-of-chicken-ovalbumin-upstream-promoter-transcription-factor-ii-and-liver-x-receptor-as-prognostic-indicators-for-human-colorectal-cancer
#1
Seong-Hoon Yun, Min-Gyoung Park, Yu-Mi Kim, Mee-Sook Roh, Joo-In Park
Cholesterol increases the risk of colorectal cancer. Liver X receptor (LXR), retinoid X receptor (RXR)α and sterol regulatory element binding protein (SREBP)-1c are transcriptional regulators of lipid metabolism. Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) serves an essential role in angiogenesis and development, but its role in cancer is controversial. The expression of COUP-TFII, LXR, RXRα and SREBP-1c in colorectal cancer, as well as their association with clinicopathologic features, was assessed, and their utility as prognostic indicators in colorectal cancer evaluated...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28943868/microbial-shifts-in-the-intestinal-microbiota-of-salmonella-infected-chickens-in-response-to-enrofloxacin
#2
Jun Li, Haihong Hao, Guyue Cheng, Chunbei Liu, Saeed Ahmed, Muhammad A B Shabbir, Hafiz I Hussain, Menghong Dai, Zonghui Yuan
Fluoroquinolones (FQs) are important antibiotics used for treatment of Salmonella infection in poultry in many countries. However, oral administration of fluoroquinolones may affect the composition and abundance of a number of bacterial taxa in the chicken intestine. Using 16S rRNA gene sequencing, the microbial shifts in the gut of Salmonella infected chickens in response to enrofloxacin treatments at different dosages (0, 0.1, 4, and 100 mg/kg b.w.) were quantitatively evaluated. The results showed that the shedding levels of Salmonella were significantly reduced in the high dosage group as demonstrated by both the culturing method and 16S rRNA sequencing method...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28943766/characterization-of-the-biodiversity-of-the-spoilage-microbiota-in-chicken-meat-using-next-generation-sequencing-and-culture-dependent-approach
#3
Hee Soo Lee, Mirae Kwon, Sunhak Heo, Min Gon Kim, Geun-Bae Kim
This study investigated the psychrotrophic bacteria isolated from chicken meat to characterize their microbial composition during refrigerated storage. The bacterial community was identified by the Illumina MiSeq method based on bacterial DNA extracted from spoiled chicken meat. Molecular identification of the isolated psychrotrophic bacteria was carried out using 16S rDNA sequencing and their putrefactive potential was investigated by the growth at low temperature as well as their proteolytic activities in chicken meat...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/28943476/consumers-willingness-to-pay-for-reprocessed-fried-chicken-a-way-of-reducing-uneaten-food
#4
Yukichika Kawata, Satoko Kubota
Substantial food loss and waste occur worldwide; approximately one third of produced food is lost or wasted annually, which worsens problems such as starvation and environmental degradation. This study examines the possibility of selling reprocessed meat products made from raw meat near its sell-by date, using Japanese fried chicken as an example. If reprocessed products are accepted by consumers, this will significantly contribute to reducing food waste in grocery stores. Because reprocessing does not require a reduction in current meat consumption, selling reprocessed foods is a more feasible and realistic way to reduce food waste compared to other initiatives, such as "Meat Free Monday...
September 21, 2017: Appetite
https://www.readbyqxmd.com/read/28943447/anti-allergic-effects-of-the-ethanol-extract-of-syzygium-formosum-wall-masam-leaves-and-its-immunoregulatory-mechanisms
#5
Nguyen Thi Minh Nguyet, Maria Lomunova, Vu Thi Phuong Duyen, Le Ba Vinh, Young Ho Kim, Jong Seong Kang, Inkyu Hwang
ETHNOPHARMACOLOGICAL RELEVANCE: As documented in a Vietnamese traditional medical encyclopedia, Syzygium formosum (Wall.) Masam leaves have been routinely used among indigenous Vietnamese people for treatment of various allergy-like symptoms including dermatitis and rhinitis. AIM OF THE STUDY: Anti-allergic activity of S. formosum leaves was examined with a mouse model of chicken ovalbumin (cOVA)-induced food allergy, and mechanisms underlying the anti-allergic effect were explored...
September 21, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28943001/melatonin-modulates-monochromatic-light-induced-melatonin-receptor-expression-in-the-hypothalamus-of-chicks
#6
Liwei Zhang, Funing Chen, Jing Cao, Yulan Dong, Zixu Wang, Yaoxing Chen
To study the mechanism of the effect of monochromatic light on physiological function in chicken, a total of 192 newly hatched chicks were randomly divided into intact, sham-operated and pinealectomy groups then exposed to white light (WL), red light (RL), green light (GL) and blue light (BL) using a light-emitting diode (LED) system for two weeks. At P14, the hypothalami were immediately collected for immunohistochemical staining of melatonin receptor subtypes (Mel1a and Mel1b) and detection of Mel1a and Mel1b expressions using RT-PCR and western blot...
September 21, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28942997/facile-and-green-fabrication-of-cation-exchange-membrane-adsorber-with-unprecedented-adsorption-capacity-for-protein-purification
#7
M Kamran Khan, Jianquan Luo, Rashid Khan, Jinxin Fan, Yinhua Wan
Fabricating membrane adsorbers with high adsorption capacity and appreciable throughput for the separation and purification of protein products is challenging in biomedical and pharmaceutical industries. Herein, we report the synthesis of a novel membrane adsorber by functionalizing a nylon microfiltration membrane with alginate dialdehyde (ADA) followed by sulphonic addition, without any solvent usage, and its successful application in the purification of lysozyme. Taking advantage of abundant dual cation exchange (CEX) groups on sulphonic-ADA (S-ADA) ligands, this novel S-ADA-nylon membrane adsorber showed an unprecedented static binding capicity of 286mg/mL for lysozyme adsorption...
September 15, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28942844/comparative-genomics-of-lactobacillus-salivarius-strains-focusing-on-their-host-adaptation
#8
Jun-Yeong Lee, Geon Goo Han, Eun Bae Kim, Yun-Jaie Choi
Lactobacillus salivarius is an important member of the animal gut microflora and is a promising probiotic bacterium. However, there is a lack of research on the genomic diversity of L. salivarius species. In this study, we generated 21 L. salivarius draft genomes, and investigated the pan-genome of L. salivarius strains isolated from humans, pigs and chickens using all available genomes, focusing on host adaptation. Phylogenetic clustering showed a distinct categorization of L. salivarius strains depending on their hosts...
December 2017: Microbiological Research
https://www.readbyqxmd.com/read/28942640/development-of-an-indirect-competitive-elisa-for-glycocholic-acid-based-on-chicken-single-chain-variable-fragment-scfv-antibodies
#9
Xiping Cui, Natalia Vasylieva, Panpan Wu, Bogdan Barnych, Jun Yang, Ding Shen, Qiyi He, Shirley J Gee, Suqing Zhao, Bruce D Hammock
Glycocholic acid (GCA) is an important metabolite of bile acids, whose urine levels are expected to be a specific diagnostic biomarker for hepatocellular carcinoma (HCC). A high-throughput immunoassay for determination of GCA would be of significant advantage and useful for primary diagnosis, surveillance and early detection of HCC. Single-chain variable fragment (scFv) antibodies have several desirable characteristics and are an attractive alternative to traditional antibodies for the immunoassay. Because chicken antibodies possess single heavy and light variable functional domains, it is an ideal framework for simplified generation of recombinant antibodies for GCA detection...
September 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28942500/evaluation-of-immunostimulatory-and-immunotherapeutic-effects-of-tropical-mushroom-lentinus-edodes-against-eimeriasis-in-chicken
#10
Muhammad Irfan Ullah, Masood Akhtar, Mian Muhammad Awais, Muhammad Irfan Anwar, Kashfa Khaliq
Mushrooms (Lentinus edodes) were processed for hot water (HWE), methanolic (ME), and polysaccharide (PSE) extracts. Polysaccharides were isolated through ion exchange (DEAE cellulose) and size exclusion (Sephadex G-100) chromatography. Monosaccharides including maltose (0.282%), glucose (0.113%), and mannose (0.451%) were identified, qualitatively and quantitatively, from the isolated polysaccharides through high-performance liquid chromatography. The whole study was divided into two experiments. Experiment 1 was meant for the evaluation of HWE and ME; whereas, experiment 2 was meant for the evaluation of PSE for immunostimulatory and immunotherapeutic activities...
September 24, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28941926/methionine-sulfoxide-reductase-a-of-salmonella-typhimurium-interacts-with-several-proteins-and-abets-in-its-colonization-in-the-chicken
#11
Ratanti Sarkhel, Parvathy Rajan, Ashish Kumar Gupta, Manoj Kumawat, Pranjali Agarwal, Arijit Shome, Manish Mahawar Lalsangpuii
Defending phagocyte generated oxidants is the key for survival of Salmonella Typhimurium (S. Typhimurium) inside the host. Met residues are highly prone to oxidation and convert into methionine sulfoxide (Met-SO). Methionine sulfoxide reductase (Msr) can repair Met-SO to Met thus restoring the function(s) of Met-SO containing proteins. Using pull down method we have identified several MsrA interacting proteins in the S. Typhimurium, one of them was malate synthase (MS). MS is an enzyme of glyoxylate cycle. This cycle is essential for survival of S...
September 20, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28941903/migration-of-salmonella-serotypes-heidelberg-and-enteritidis-in-previously-frozen-chicken-breast-meat
#12
Kamila Tozzo, Américo F G Neto, Katherinne M Spercoski, Milton Ronnau, Vanessa M Soares, Luciano S Bersot
Salmonella spp. have been shown to migrate to the internal regions of meat cuts. Storage conditions and the presence of proteolytic microbiota can influence this process. Our study assessed the impact of storage time, temperature, and the presence of proteolytic psychrotrophic bacteria on migration. Samples of previously frozen chicken breast with skin and bone were then sterilized using gamma ray irradiation and a cobalt-60 source (11 KGy) and them were inoculated with cultures of S. Enteritidis, S. Enteritidis and psychrotrophs, S...
February 2018: Food Microbiology
https://www.readbyqxmd.com/read/28941621/safety-and-efficacy-of-a-mycoplasma-gallisepticum-oppd-knockout-mutant-as-a-vaccine-candidate
#13
Chi-Wen Tseng, Chien-Ju Chiu, Anna Kanci, Amir H Noormohammadi, Glenn F Browning, Philip F Markham
Control of the important poultry pathogen Mycoplasma gallisepticum is highly dependent on safe and efficacious attenuated vaccines. In order to assess a novel vaccine candidate we evaluated the safety and efficacy of the M. gallisepticum mutant 26-1. The oppD1 gene in this mutant has been interrupted by a signature-tagged transposon and previous studies have shown that it can colonise the respiratory tract of chickens without inducing significant disease. The capacity of the oppD1 mutant to induce protective immunity in the respiratory tract after vaccination by eye-drop was assessed by challenging vaccinated birds with an aerosol of the virulent M...
September 20, 2017: Vaccine
https://www.readbyqxmd.com/read/28941132/infectious-dose-dependent-accumulation-of-live-highly-pathogenic-avian-influenza-h5n1-virus-in-chicken-skeletal-muscle-implications-for-public-health
#14
G Vasudevan, P R Vanamayya, S Nagarajan, K Rajukumar, S Suba, G Venketash, C Tosh, R Sood, R H Nissly, S V Kuchipudi
Highly pathogenic avian influenza viruses (HPAIV) of H5N1 subtype are a major global threat to poultry and public health. Export of poultry products, such as chicken and duck meat, is a known source for the cross-boundary spread of HPAI H5N1 viruses. Humans get infected with HPAI H5N1 viruses either by close contact with infected poultry or through consumption of fresh/undercooked poultry meat. Skeletal muscle is the largest soft tissue in chicken that has been shown to contain virus during systemic HPAIV infection and supports productive virus infection...
September 20, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28940934/the-patterns-of-birthmarks-suggest-a-novel-population-of-melanocyte-precursors-arising-around-the-time-of-gastrulation
#15
REVIEW
Veronica A Kinsler, Lionel Larue
Systematic work in the mouse and chicken has mapped out two neural crest-derived pathways of melanocyte precursor migration. With these in mind, this study reappraises the patterns of congenital pigmentary disorders in humans, and identifies three recurrent patterns consistent across genetically-different diseases. Only two of these are seen in diseases known to be melanocyte cell-autonomous. The segmental pattern correlates well with the classical dorsolateral population from animal studies, demonstrating respect of the midline, craniocaudal axial mixing, unilateral migration and involvement of key epidermally-derived structures...
September 23, 2017: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/28940676/asymmetric-development-of-the-nervous-system
#16
REVIEW
Amel Alqadah, Yi-Wen Hsieh, Zachery D Morrissey, Chiou-Fen Chuang
The human nervous system consists of seemingly symmetric left and right halves. However, closer observation of the brain reveals anatomical and functional lateralization. Defects in brain asymmetry correlate with several neurological disorders, yet our understanding of the mechanisms used to establish lateralization in the human central nervous system is extremely limited. Here, we review left-right asymmetries within the nervous system of humans and several model organisms, including rodents, zebrafish, chickens, Xenopus, Drosophila, and the nematode Caenorhabditis elegans...
September 23, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28940019/genome-sequence-of-a-mallard-duck-origin-cyclovirus-duacyv-1
#17
Enikő Fehér, Eszter Kaszab, Barbara Forró, Krisztina Bali, Szilvia Marton, György Lengyel, Krisztián Bányai
The genome sequence of a novel avian cyclovirus is described in this study. The genome size and orientation of predicted genes was similar to those described in other vertebrate and insect origin cycloviruses. The greatest genome sequence identity was shared with a dragonfly cyclovirus (nt, 60.6%). Phylogenetic analysis showed marginal relatedness with another avian cyclovirus, the chicken associated cyclovirus 1. In contrast, along a short fragment of the replication-associated protein coding gene (rep) (spanning nt 1240-1710) the duck origin cyclovirus was very similar to human origin and honey bee origin rep sequences (human - TN4, 98%; honey bee - hb10, 100%)...
September 22, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28940009/print-to-detect-a-rapid-and-ultrasensitive-phage-based-dipstick-assay-for-foodborne-pathogens
#18
Hany Anany, Lubov Brovko, Noha K El Dougdoug, Jennifer Sohar, Heather Fenn, Nada Alasiri, Tarik Jabrane, Patrice Mangin, M Monsur Ali, Balamurali Kannan, Carlos D M Filipe, Mansel W Griffiths
Foodborne pathogens are a burden to the economy and a constant threat to public health. The ability to rapidly detect the presence of foodborne pathogens is a vital component of any strategy towards establishing a safe and secure food supply chain. Bacteriophages (phages) are viruses capable of infecting and replicating within bacteria in a strain-specific manner. The ubiquitous and selective nature of phages makes them ideal for the detection and biocontrol of bacteria. Therefore, the objective of this research was to develop and test a phage-based paper dipstick biosensor for the detection of various foodborne pathogens in food matrices...
September 22, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28939610/co-administration-of-the-campylobacter-jejuni-n-glycan-based-vaccine-with-probiotics-improves-vaccine-performance-in-broiler-chickens
#19
H Nothaft, M E Perez-Muñoz, G J Gouveia, R M Duar, J J Wanford, L Lango-Scholey, C G Panagos, V Srithayakumar, G S Plastow, C Coros, C D Bayliss, A S Edison, J Walter, C M Szymanski
Source attribution studies report that consumption of contaminated poultry is the primary source for acquiring human campylobacteriosis. Oral administration of an engineered Escherichia coli strain expressing the Campylobacter jejuni N-glycan reduces bacterial colonization in specific-pathogen-free leghorn chickens, but only a fraction of birds respond to vaccination. Optimizing the vaccine for commercial broiler chickens has great potential to prevent pathogen entry into the food chain. Here, we tested the same vaccination approach in broilers and observed similar efficacy in pathogen load reduction, stimulation of host IgY response, lack of C...
September 22, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28939604/genetic-diversity-of-infectious-laryngotracheitis-virus-during-in-vivo-co-infection-parallels-viral-replication-and-arises-from-recombination-hot-spots-within-the-genome
#20
Carlos A Loncoman, Carol A Hartley, Mauricio J C Coppo, Paola K Vaz, Andrés Diaz-Méndez, Glenn F Browning, Maricarmen García, Stephen Spatz, Joanne M Devlin
Recombination is a feature of many alphaherpesviruses that infect people and animals. Infectious laryngotracheitis virus (ILTV - Gallid alphaherpesvirus 1), causes respiratory disease in chickens, resulting in significant production losses in poultry industries worldwide. Natural (field) ILTV recombination is widespread, particularly recombination between attenuated ILTV vaccine strains to create virulent viruses. These virulent recombinants have had a major impact on animal health. Recently, the development of a single nucleotide polymorphism (SNP) genotyping assay for ILTV has helped to understand ILTV recombination under laboratory settings...
September 22, 2017: Applied and Environmental Microbiology
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