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https://www.readbyqxmd.com/read/28812532/spontaneous-experimentally-induced-and-transmissible-aa-amyloidosis-in-japanese-quail-coturnix-japonica
#1
Yumi Nakayama, Junichi Kamiie, Gen Watanabe, Kazuhiko Suzuki, Tomoaki Murakami
The authors describe a spontaneous case of amyloid A (AA) amyloidosis in an adult female Japanese quail ( Coturnix japonica). The bird developed AA amyloidosis secondary to chronic peritonitis caused by a Gram-negative bacillus infection. Mild amyloid deposition was also identified in the intestinal tract of apparently healthy adult individuals, suggesting that quail may develop intestinal amyloidosis with age. Based on these observations, it was hypothesized that quail can develop AA amyloidosis following inflammatory stimulation with lipopolysaccharide (LPS)...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28812373/evaluation-of-factors-in-development-of-vis-nir-spectroscopy-models-for-discriminating-pse-dfd-and-normal-broiler-breast-meat
#2
Hongzhe Jiang, Seung-Chul Yoon, Hong Zhuang, Wei Wang, Yi Yang
1. To evaluate the performance of visible and near-infrared (Vis/NIR) spectroscopic models for discriminating true pale, soft and exudative (PSE), normal and dark, firm and dry (DFD) broiler breast meat in different conditions of preprocessing methods, spectral ranges, characteristic wavelengths selection and water-holding capacity (WHC) indexes. 2. Quality attributes of 214 intact chicken fillets (pectoralis major), such as lightness (L*), pH, and WHC indicators including drip loss (DL), water gain (WG), and expressible fluid (EF) were measured...
August 16, 2017: British Poultry Science
https://www.readbyqxmd.com/read/28811548/ligand-binding-modes-from-low-resolution-gpcr-models-and-mutagenesis-chicken-bitter-taste-receptor-as-a-test-case
#3
Antonella Di Pizio, Louisa-Marie Kruetzfeldt, Shira Cheled-Shoval, Wolfgang Meyerhof, Maik Behrens, Masha Y Niv
Bitter taste is one of the basic taste modalities, warning against consuming potential poisons. Bitter compounds activate members of the bitter taste receptor (Tas2r) subfamily of G protein-coupled receptors (GPCRs). The number of functional Tas2rs is species-dependent. Chickens represent an intriguing minimalistic model, because they detect the bitter taste of structurally different molecules with merely three bitter taste receptor subtypes. We investigated the binding modes of several known agonists of a representative chicken bitter taste receptor, ggTas2r1...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28810864/molecular-determination-of-antimicrobial-resistance-in-escherichia-coli-isolated-from-raw-meat-in-addis-ababa-and-bishoftu-ethiopia
#4
Yohannes Equar Messele, Reta Duguma Abdi, Shimels Tikuye Yalew, Desiye Tesfaye Tegegne, Bezina Arega Emeru, Gebremeskel Mamu Werid
BACKGROUND: Consumption of meat contaminated by E. coli causes a serious illness and even death to affected individuals. Recently the emerging of antibiotic resistant foodborne E. coli poses serious public health risks worldwide. However, little is known about the antibiotic resistance profile of E. coli in Ethiopia. This study aimed to determine the prevalence and Antimicrobial resistance (AMR) status of E. coli isolated from different type of meat. METHODS: Overall 292 samples were collected from December 2015 to April 2016 from slaughterhouses to determine the prevalence and AMR of E...
August 15, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28810830/annotating-long-intergenic-non-coding-rnas-under-artificial-selection-during-chicken-domestication
#5
Yun-Mei Wang, Hai-Bo Xu, Ming-Shan Wang, Newton Otieno Otecko, Ling-Qun Ye, Dong-Dong Wu, Ya-Ping Zhang
BACKGROUND: Numerous biological functions of long intergenic non-coding RNAs (lincRNAs) have been identified. However, the contribution of lincRNAs to the domestication process has remained elusive. Following domestication from their wild ancestors, animals display substantial changes in many phenotypic traits. Therefore, it is possible that diverse molecular drivers play important roles in this process. RESULTS: We analyzed 821 transcriptomes in this study and annotated 4754 lincRNA genes in the chicken genome...
August 15, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/28810218/effect-of-cold-adapted-microbial-agent-inoculation-on-enzyme-activities-during-composting-start-up-at-low-temperature
#6
Qinghong Sun, Di Wu, Zhechao Zhang, Yue Zhao, Xinyu Xie, Junqiu Wu, Qian Lu, Zimin Wei
In order to put forward a method to promote composting start-up at low ambient temperature, the cold-adapted microbial agent (CAMA) was inoculated in chicken manure (CM), and compared the enzymes activities, including urease, proteases, β-glucosidase and invertase, with no CAMA group (CK). In this study, the temperature of CM reached 50°C in 53h, but it in CK was only around 30°C during the composting process. Moreover, the enzymes exhibited higher activity in CM than CK, indicating the effectiveness of CAMA...
August 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28809155/microbe-profile-candida-albicans-a-shape-changing-opportunistic-pathogenic-fungus-of-humans
#7
Neil A R Gow, Bhawna Yadav
(a) Morphotypes of Candida albicans (blue sector, vegetative forms; pink sector, forms related to mating or changes in ploidy). Cell types shown include: budding yeast cells; elongated conjoined yeasts forming pseudohyphae; parallel-sided hyphae; chlamydospores formed from suspensor cells; enlarged goliath cells formed under zinc deprivation (unpublished study, image from Duncan Wilson); intestinal gut form cells (image from Suzanne Noble); mating competence defined by white-grey-opaque cell transitions (images from Guanghua Huang); elongated chemotactic shmoo-mating projections leading to tetraploid zygote formation (images from David Soll and Karla Daniels); trimera formed by unequal chromosome segregation under antifungal exposure (image from Judith Berman)...
August 15, 2017: Microbiology
https://www.readbyqxmd.com/read/28809025/isolation-and-characterization-of-chicken-primordial-germ-cells-and-their-application-in-transgenesis
#8
Jae Yong Han, Bo Ram Lee
Primordial germ cells (PGCs), precursors of functional gametes, offer great promise for the use of genetic resources and transgenesis in chickens. PGCs can be isolated from the developing embryo at diverse early stages and are subsequently expandable in vitro. In vitro proliferating chicken PGCs can facilitate the production of efficient germline chimeras and transgenic chickens. Here, we describe methods to isolate and characterize PGCs from chicken embryos and their application to transgenesis.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28809024/chicken-induced-pluripotent-stem-cells-establishment-and-characterization
#9
Aurelie Fuet, Bertrand Pain
In mammals, the introduction of the OSKM (Oct4, Sox2, Klf4, and c-Myc) genes into somatic cells has allowed generating induced pluripotent stem (iPS) cells. So far, this process has been only clearly demonstrated in mammals. Here, using chicken as an avian model, we describe a set of protocols allowing the establishment, characterization, maintenance, differentiation, and injection of putative reprogrammed chicken Induced Pluripotent Stem (iPS) cells.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28809022/enhancer-analyses-using-chicken-embryo-electroporation
#10
Masanori Uchikawa, Naoko Nishimura, Makiko Iwafuchi-Doi, Hisato Kondoh
Chicken embryo electroporation is a powerful tool used to identify and analyze enhancers involved in developmental gene regulation. In this chapter, the basic procedures and underlying principles of enhancer analysis using chicken embryo electroporation are described in the following steps: (1) identification of enhancers in a wide genomic region, (2) determination of the full enhancer region, (3) definition of the core enhancer regions, and (4) analysis of a functional transcription factor binding sequences in the core region...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28809021/genetic-manipulation-of-the-avian-urogenital-system-using-in-ovo-electroporation
#11
Claire E Hirst, Olivier Serralbo, Katie L Ayers, Kelly N Roeszler, Craig A Smith
One of the advantages of the avian embryo as an experimental model is its in ovo development and hence accessibility for genetic manipulation. Electroporation has been used extensively in the past to study gene function in chicken and quail embryos . Readily accessible tissues such as the neural tube, somites, and limb bud, in particular, have been targeted. However, more inaccessible tissues, such as the embryonic urogenital system , have proven more challenging to study. Here, we describe the use of in ovo electroporation of TOL2 vectors or RCASBP avian viral vectors for the rapid functional analysis of genes involved in avian sex determination and urogenital development ...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28809017/crispr-cas9-in-the-chicken-embryo
#12
Valérie Morin, Nadège Véron, Christophe Marcelle
Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms. We recently showed that this technique can be combined with in vivo electroporation to inhibit the function of genes of interest in somatic cells of the developing chicken embryo. We present here a simplified version of the previously described technique that leads to effective gene loss-of-function...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28809016/application-of-a-cage-method-to-an-avian-development-study
#13
Ruslan Deviatiiarov, Marina Lizio, Oleg Gusev
Cap analysis of gene expression (CAGE) is a convenient approach for genome-wide identification of promoter regions at single base-pair resolution level and accurate expression estimation of the corresponding transcripts. Depending on the initial biomaterial amount and sequencing technology, different computational pipelines for data processing are available, as well as variations of the CAGE protocol that improve sensitivity and accuracy. Therefore, this chapter elucidates the key steps of sample preparation, sequencing, and data analysis via an example of a promoter expression estimation study in chicken development...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28809015/systems-biology-analyses-in-chicken-workflow-for-transcriptome-and-chip-seq-analyses-using-the-chicken-skin-paradigm
#14
Yung-Chih Lai, Randall B Widelitz, Cheng-Ming Chuong
With advances in molecular biology, various biological phenomena can now be explored at higher resolution using mRNA sequencing (RNA-Seq) and chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-Seq), two powerful high-throughput next-generation sequencing (NGS) technologies. While methods are used widely in mouse, human, etc., less information is available in other animals, such as the chicken. Here we assemble a workflow of the RNA-Seq and ChIP-Seq analyses for the chicken studies using chicken skin appendage tissue as an example...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28808846/age-related-changes-in-morphology-of-left-atrial-appendage-in-patients-with-atrial-fibrillation
#15
Yukina Hirata, Kenya Kusunose, Hirotsugu Yamada, Rikuto Shimizu, Yuta Torii, Susumu Nishio, Yoshihito Saijo, Shoichiro Takao, Takeshi Soeki, Masataka Sata
The purpose of this study was to evaluate the relationship between age and frequency of left atrial appendage (LAA) morphology in patients with atrial fibrillation (AF) compared with sinus rhythm (SR). We enrolled 145 AF patients, and 199 SR patients for the control group without any cardiovascular disease. LAA volume index (LAAVi) and morphology were assessed by electrocardiogram-gated computed tomography angiography. LAA morphology was classified into "chicken wing" or "non-chicken wing" according to the previously described classification...
August 14, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28808778/audiogram-of-the-mallard-duck-anas-platyrhynchos-from-16%C3%A2-hz-to-9%C3%A2-khz
#16
Evan M Hill
The pure-tone thresholds of three mallard ducks were determined from 16 Hz to 9 kHz. The purpose was to determine whether the mallard duck hears infrasound, which then may potentially be used for navigation, similar to how it is proposed that pigeons use it for homing. At a level of 60 dB sound pressure level (re 20 μN/m(2)), their hearing range extends 6.85 octaves from 66 Hz to 7.6 kHz, with a best sensitivity of 12.5 dB at 2 kHz. However, at no frequency, including the lowest tested, were the ducks' thresholds lower than those of humans...
August 14, 2017: Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology
https://www.readbyqxmd.com/read/28808158/the-influence-of-the-gut-microbiota-composition-on-campylobacter-jejuni-colonization-in-chickens
#17
Zifeng Han, Thomas Willer, Li Li, Colin Pielsticker, Ivan Rychlik, Philippe Velge, Bernd Kaspers, Silke Rautenschlein
Campylobacter jejuni (C. jejuni)-host-interaction may be affected by the host's gut microbiota through competitive exclusion, metabolites or modification of the immune response. To understand this interaction C. jejuni colonization and local immune responses were compared in chickens with different gut microbiota composition. Birds were treated with an antibiotic cocktail (AT) (Experiment 1 and 2) or raised under germ-free (GF) conditions (Experiment 3). At 18 days post hatch (dph), they were either orally inoculated with 10(4) colony forming units (CFU) of C...
August 14, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28808070/chicken-or-the-egg-hyperuricemia-insulin-resistance-and-hypertension
#18
EDITORIAL
Jesse Dawson, Annick Wyss
No abstract text is available yet for this article.
August 14, 2017: Hypertension
https://www.readbyqxmd.com/read/28807781/engrailed-controls-epaxial-hypaxial-muscle-innervation-and-the-establishment-of-vertebrate-three-dimensional-mobility
#19
Mohi U Ahmed, Ashish K Maurya, Louise Cheng, Erika C Jorge, Frank R Schubert, Pascal Maire, M Albert Basson, Philip W Ingham, Susanne Dietrich
Chordates are characterised by contractile muscle on either side of the body that promotes movement by side-to-side undulation. In the lineage leading to modern jawed vertebrates (crown group gnathostomes), this system was refined: body muscle became segregated into distinct dorsal (epaxial) and ventral (hypaxial) components that are separately innervated by the medial and hypaxial motors column, respectively, via the dorsal and ventral ramus of the spinal nerves. This allows full three-dimensional mobility, which in turn was a key factor in their evolutionary success...
August 11, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28807621/antitoxin-therapy-of-natural-avian-botulism-outbreaks-occurred-in-brazil
#20
Rodrigo O S Silva, Sandra Y M Gómez, Lilian B Medeiros, Marcus V R Marques, Aila S G Silva, Elisabeth N Mureb, Carlos A Oliveira Junior, Samantha M Favoretto, Francisco C F Lobato, Nelson R S Martins
Botulism commonly affects water birds and it has recently been observed to be emerging in poultry production. In the present work, outbreaks of botulism in wild native species, such as the black-fronted Piping-guan (Aburria jacutinga), wild duck (Cairina moschata) and its crosses with mallard (Anas platyrhynchos), and domestic chickens (Gallus gallus domesticus) are described. Following treatments with a commercial botulism antitoxin CD, 28 (96.5%) out of 29 animals fully recovered after 24-72 h. The antitoxin therapy was shown to be a useful option for the treatment of affected birds, including those that were severely affected...
August 12, 2017: Anaerobe
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