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https://www.readbyqxmd.com/read/29793206/effects-on-the-hepatic-transcriptome-of-chicken-embryos-in-ovo-exposed-to-phenobarbital
#1
Jiahua Guo, Shohei Ito, Hoa Thanh Nguyen, Kimika Yamamoto, Hisato Iwata
This work aimed at evaluating the toxic effects of in ovo exposure to phenobarbital (PB) and unveiling the mode of action by transcriptome analysis in the embryonic liver of a model avian species, chicken (Gallus gallus). Embryos were initially treated with saline or 1 μg PB /g egg at Hamburger Hamilton Stage (HHS) 1 (1st day), followed by 20 days of incubation to HHS 46. At 21st day, chicks that pipped successfully were euthanized and dissected for assessing the PB caused effects on phenotypes and the liver transcriptome in both genders...
May 21, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29792901/identification-and-functional-characterization-including-cytokine-production-modulation-of-the-novel-chicken-interleukin-11
#2
Anh Duc Truong, Yeojin Hong, Deivendran Rengaraj, Janggeun Lee, Kyungbaek Lee, Yeong Ho Hong
Interleukin (IL)-11 plays an important role in the immune system. However, IL-11 has not yet been characterized in avian species, including chickens. This study is the first to clone and functionally characterize chicken IL-11 (chIL-11). Multiple alignments and phylogenetic tree comparisons of chIL-11 with IL-11 proteins from other species revealed high levels of conservation and a close relationship between chicken and Japanese quail IL-11. Our results demonstrate that chIL-11 was a functional ligand of IL-11RA and IL-6ST in chicken HD11 and OU2 cell lines, as well as activated and regulated JAK-STAT, NF-κB, PI3K/AKT, and MAPK signaling pathways in chicken cell lines...
May 21, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29792856/comparison-of-the-3-d-patterns-of-the-parasympathetic-nervous-system-in-the-lung-at-late-developmental-stages-between-mouse-and-chicken
#3
Tadayoshi Watanabe, Ryo Nakamura, Yuta Takase, Etsuo A Susaki, Hiroki R Ueda, Ryosuke Tadokoro, Yoshiko Takahashi
Although the basic schema of the body plan is similar among different species of amniotes (mammals, birds, and reptiles), the lung is an exception. Here, anatomy and physiology are considerably different, particularly between mammals and birds. In mammals, inhaled and exhaled airs mix in the airways, whereas in birds the inspired air flows unidirectionally without mixing with the expired air. This bird-specific respiration system is enabled by the complex tubular structures called parabronchi where gas exchange takes place, and also by the bellow-like air sacs appended to the main part of the lung...
May 21, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29792781/is-bla-ctx-m-1-riding-the-same-plasmid-among-horses-in-sweden-and-france
#4
Agnese Lupo, Marisa Haenni, Estelle Saras, Joanna Gradin, Jean-Yves Madec, Stefan Börjesson
A predominance of the blaCTX-M-1 /IncHI1 plasmid combination in horses has been reported in Czech-Republic, Denmark, and The Netherlands. To clarify a possible specific plasmid epidemiology of blaCTX-M-1 in horses in a European perspective, a collection of 74 extended-spectrum beta-lactamase-producing Escherichia coli recovered from diseased horses in France and Sweden during the period 2009-2014 was investigated in respect of their genetic relatedness, plasmid content, and molecular features. Overall, 80% of E...
May 24, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29792308/logical-modelling-uncovers-developmental-constraints-for-primary-sex-determination-of-chicken-gonads
#5
Lucas Sánchez, Claudine Chaouiya
In the chicken, sex determination relies on a ZZ (male)/ZW (female) chromosomal system, but underlying mechanisms are still not fully understood. The Z-dosage and the dominant W-chromosome hypotheses have been proposed to underlie primary sex determination. We present a modelling approach, which assembles the current knowledge and permits exploration of the regulation of this process in chickens. Relying on published experimental data, we assembled a gene network, which led to a logical model that integrates both the Z-dosage and dominant W hypotheses...
May 2018: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/29792171/embryonic-transcriptome-and-proteome-analyses-on-hepatic-lipid-metabolism-in-chickens-divergently-selected-for-abdominal-fat-content
#6
Wei Na, Yuan-Yuan Wu, Peng-Fei Gong, Chun-Yan Wu, Bo-Han Cheng, Yu-Xiang Wang, Ning Wang, Zhi-Qiang Du, Hui Li
BACKGROUND: In avian species, liver is the main site of de novo lipogenesis, and hepatic lipid metabolism relates closely to adipose fat deposition. Using our fat and lean chicken lines of striking differences in abdominal fat content, post-hatch lipid metabolism in both liver and adipose tissues has been studied extensively. However, whether molecular discrepancy for hepatic lipid metabolism exists in chicken embryos remains obscure. RESULTS: We performed transcriptome and proteome profiling on chicken livers at five embryonic stages (E7, E12, E14, E17 and E21) between the fat and lean chicken lines...
May 23, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29790657/from-low-to-high-pathogenicity-characterization-of-h7n7-avian-influenza-viruses-in-two-epidemiologically-linked-outbreaks
#7
Klaas Dietze, Annika Graaf, Timo Homeier-Bachmann, Christian Grund, Leonie Forth, Anne Pohlmann, Christa Jeske, Mattis Wintermann, Martin Beer, Franz J Conraths, Timm Harder
The ability of low pathogenic (LP) avian influenza viruses (AIV) of the subtypes H5 and H7 to mutate spontaneously to highly pathogenic (HP) variants is the main reason for their stringent control. On-the-spot evidence from the field of mutations in LPAIV to render the virus into nascent HP variants is scarce. Epidemiological investigations and molecular characterization of two spatiotemporally linked outbreaks caused by LP, and subsequently, HPAIV H7N7 in two-layer farms in Germany yielded such evidence. The outbreaks occurred within 45 days on farms 400 m apart...
May 23, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29790588/the-lncrna-casc9-and-rna-binding-protein-hnrnpl-form-a-complex-and-co-regulate-genes-linked-to-akt-signaling
#8
Marcel Klingenberg, Matthias Groß, Ashish Goyal, Maria Polycarpou-Schwarz, Thilo Miersch, Anne-Sophie Ernst, Jörg Leupold, Nitin Patil, Uwe Warnken, Heike Allgayer, Thomas Longerich, Peter Schirmacher, Michael Boutros, Sven Diederichs
The identification of viability-associated long non-coding RNAs (lncRNA) might be a promising rationale for new therapeutic approaches in liver cancer. Here, we applied the first RNAi screening approach in hepatocellular carcinoma (HCC) cell lines to find viability-associated lncRNAs. Among the multiple identified lncRNAs with a significant impact on HCC cell viability, we selected CASC9 (Cancer Susceptibility 9) due to the strength of its phenotype, expression, and upregulation in HCC versus normal liver. CASC9 regulated viability across multiple HCC cell lines as shown by CRISPR interference, single siRNA- and siPOOL-mediated depletion of CASC9...
May 23, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29789988/growth-performance-blood-parameters-carcass-characteristics-and-meat-quality-traits-in-potchefstroom-koekoek-chickens-fed-lippia-javanica-leaf-meal
#9
T B Matshogo, V Mlambo, U Marume, N Sebola
Lippia javanica leaves may have nutraceutical bioactivity and thus could be a viable alternative to antibiotic growth promoters in poultry diets. To test this hypothesis, a feeding trial was carried out to determine the effects of dietary inclusion of Lippia javanica leaf meal (LJ) on growth performance, blood parameters, carcass characteristics and meat quality of indigenous Potchefstroom Koekoek (PK) chickens. The leaf meal was used to dilute a commercial chicken grower diet at a rate of 25 or 50 g/kg diet...
May 22, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29789643/changes-in-pituitary-gene-expression-may-underlie-multiple-domesticated-traits-in-chickens
#10
Amir Fallahshahroudi, Pia Løtvedt, Johan Bélteky, Jordi Altimiras, Per Jensen
Domesticated animals share a unique set of morphological and behavioral traits, jointly referred to as the domesticated phenotype. Striking similarities amongst a range of unrelated domesticated species suggest that similar regulatory mechanisms may underlie the domesticated phenotype. These include color pattern, growth, reproduction, development and stress response. Although previous studies have focused on the brain to find mechanisms underlying domestication, the potential role of the pituitary gland as a target of domestication is highly overlooked...
May 22, 2018: Heredity
https://www.readbyqxmd.com/read/29789009/correction-to-antibiotic-resistance-pattern-and-virulence-genes-content-in-avian-pathogenic-escherichia-coli-apec-from-broiler-chickens-in-chitwan-nepal
#11
Manita Subedi, Rebanta Kumar Bhattarai, Bhuminand Devkota, Sarita Phuyal, Himal Luitel
The original article [1] contains errors in author panels and their contributions, errors in both the Methodology and the Results sections, and errors with respect to funding sources. The affected sections of the manuscript and their respective regions of corrected text can be viewed ahead.
May 22, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29788909/whole-genome-analysis-reveals-the-diversity-and-evolutionary-relationships-between-necrotic-enteritis-causing-strains-of-clostridium-perfringens
#12
Jake A Lacey, Theodore R Allnutt, Ben Vezina, Thi Thu Hao Van, Thomas Stent, Xiaoyan Han, Julian I Rood, Ben Wade, Anthony L Keyburn, Torsten Seemann, Honglei Chen, Volker Haring, Priscilla A Johanesen, Dena Lyras, Robert J Moore
BACKGROUND: Clostridium perfringens causes a range of diseases in animals and humans including necrotic enteritis in chickens and food poisoning and gas gangrene in humans. Necrotic enteritis is of concern in commercial chicken production due to the cost of the implementation of infection control measures and to productivity losses. This study has focused on the genomic analysis of a range of chicken-derived C. perfringens isolates, from around the world and from different years. The genomes were sequenced and compared with 20 genomes available from public databases, which were from a diverse collection of isolates from chickens, other animals, and humans...
May 22, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29788438/efficacy-of-polymers-from-spontaneous-carotenoid-oxidation-in-reducing-necrotic-enteritis-in-broilers
#13
M Kang, J-Y Oh, S-Y Cha, W-I Kim, H-S Cho, H-K Jang
This study evaluated the preventive effect of the spontaneous oxidation of β-carotene (OxC-beta) in broiler chickens with necrotic enteritis by Clostridium perfringens taking into consideration various parameters including clinical signs, body weight, intestinal lesion severity, and bacterial enumeration. The mean body weight of the OxC-beta treatment groups increased significantly (P < 0.05) compared to that of the C. perfringens challenge group. Intestinal lesion scores due to C. perfringens infection were significantly alleviated by OxC-beta treatment (P < 0...
May 18, 2018: Poultry Science
https://www.readbyqxmd.com/read/29788420/effects-of-rearing-systems-on-reproductive-hormones-secretion-and-their-receptors-gene-expression-in-xianju-chickens-under-summer-conditions
#14
Z Z Yin, X Y Dong, H Y Cao, H G Mao, Y Z Ma
Previous study in our lab showed that indigenous Xianju chickens from free-range system (FRS) under summer conditions had lower egg production than those from conventional cage rearing system (CRS). The objective of this study was to preliminarily determine the FRS-dependent mechanism of depressing laying performance according to determining the effect of rearing systems on reproductive hormones secretion and their receptors mRNA expression in Xianju chickens reared under summer conditions. A total of 360 indigenous Xianju chickens were randomly allocated to CRS and FRS groups, each of which included 5 replicates of 36 hens...
May 18, 2018: Poultry Science
https://www.readbyqxmd.com/read/29788386/diversity-not-uniformity-slaughter-and-electrical-waterbath-stunning-procedures-in-belgian-slaughterhouses
#15
G Devos, C P H Moons, K Houf
Data on slaughter and stunning protocols in Belgian poultry abattoirs were collected, and subsequently the survival rate after electrical waterbath stunning in 1,400 animals across 7 selected slaughterhouses was determined. The majority of the abattoirs applied electrical waterbath stunning (72%), whereas the other methods were gas stunning (13%), head-only stunning (6%), and dry stunning (3%). In 6% of the slaughterhouses, the birds were killed without previous stunning, according to religious rites (i.e., ritual or religious slaughter)...
May 19, 2018: Poultry Science
https://www.readbyqxmd.com/read/29788333/isolation-and-characterization-of-subgroup-j-avian-leukosis-virus-associated-with-hemangioma-in-commercial-hy-line-chickens
#16
Fanfeng Meng, Qiuchen Li, Yubiao Zhang, Zhizhong Cui, Shuang Chang, Peng Zhao
There was an outbreak of hemangioma associated with avian leukosis virus subgroup J (ALV-J) between 2006 and 2010 in China in commercial layer chickens. Recently, severe hemangiomas broke out in Hy-Line layer chickens on a poultry farm in 2017 where ALV was eradicated earlier. Six isolates of ALV-J, named SDAU1701-SDAU1706, were characterized by virus isolation and sequence analysis of the complete proviral genomes. Avian leukosis virus subgroup J was identified by an immunofluorescence assay with monoclonal antibody JE9, whereas Marek's disease virus or reticuloendotheliosis virus was not detected...
May 19, 2018: Poultry Science
https://www.readbyqxmd.com/read/29788213/carcass-and-meat-quality-of-dual-purpose-chickens-lohmann-dual-belgian-malines-schweizerhuhn-in-comparison-to-broiler-and-layer-chicken-types
#17
S Mueller, M Kreuzer, M Siegrist, K Mannale, R E Messikommer, I D M Gangnat
Currently, there is an intensive ethical discussion about the practice of culling day-old layer cockerels. One solution to avoid this practice could be using dual-purpose types, where males are fattened for meat and females used for egg production. The aim of the present study was to compare fattening performance, carcass conformation, and composition as well as meat quality of Lohmann Dual, a novel dual-purpose type, and 2 traditional dual-purpose types (Belgian Malines and Schweizerhuhn) with 2 broiler types and 1 layer type (Lohmann Brown Plus)...
May 18, 2018: Poultry Science
https://www.readbyqxmd.com/read/29788199/influences-of-trace-mineral-nutrition-and-maternal-flock-age-on-broiler-embryo-bone-development
#18
C A Torres, D R Korver
At hatch, the chick skeleton is a miniature of that of the adult bird. The hen deposits calcium, phosphorus, and trace minerals (copper, zinc, and manganese) along with vitamin D into the egg to allow development of the embryonic skeleton. The main source of calcium is the eggshell, whereas phosphorus, trace minerals, and vitamin D are mainly derived from the yolk. Calcium is absorbed from the eggshell and transferred to the embryo and yolk through the chorioallantoic membrane, whereas phosphorus and trace minerals are simultaneously mobilized by the yolk sac membrane...
May 21, 2018: Poultry Science
https://www.readbyqxmd.com/read/29788183/membrane-rafts-regulate-sperm-acrosome-reaction-via-camp-dependent-pathway-in-chickens-gallus-gallus-domesticus
#19
Chathura Priyadarshana, Atsushi Tajima, Naoto Ishikawa, Atsushi Asano
Both transcriptionally and translationally inactive sperm need preassembled pathways into specific cellular compartments to function. Although initiation of the acrosome reaction (AR) involves several signaling pathways including protein kinase A (PKA) activation, how these are regulated remains poorly understood in avian sperm. Membrane rafts are specific membrane regions enriched in sterols and functional proteins and play important roles in diverse cellular processes, including signal transduction. Our recent studies on chicken sperm demonstrated that membrane rafts exist and play a role in multistage fertilization...
May 18, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29788161/new-reagents-for-poultry-research-preparation-purification-and-in-vitro-evaluation-of-non-pegylated-and-mono-pegylated-chicken-prolactin
#20
Ewa Oclon, Gili Solomon, Anna Hrabia, Shelly Druyan, Zvi Hayouka, Arieh Gertler
Recombinant chicken prolactin (chPRL), expressed in Escherichia coli and purified as a monomer, was successfully PEGylated and purified to homogeneity as a mono-PEGylated protein (PEG-chPRL). Its biological activity was estimated by its ability to interact with human prolactin receptor extracellular domain (hPRLR-ECD) and stimulate PRLR-mediated proliferation in Nb2-11C cells. PEG-chPRL activity in a cell bioassay was 10-fold lower than that of non-PEGylated chPRL, but only 2-fold lower in a binding assay to hPRLR-ECD...
May 18, 2018: Poultry Science
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