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https://www.readbyqxmd.com/read/28642682/neuritin-inhibits-notch-signaling-through-interacted-with-neuralized-to-promote-the-neurite-growth
#1
Pan Zhang, Xing Luo, Zheng Guo, Anying Xiong, Hongchang Dong, Qiao Zhang, Chunyan Liu, Jingling Zhu, Haiyan Wang, Na Yu, Jinli Zhang, Yu Hong, Lei Yang, Jin Huang
Neuritin plays a key role in neural development and regeneration by promoting neurite outgrowth and synapse maturation. However, the mechanism of neuritin in modulating neurite growth has not been elucidated. Here, using yeast two-hybrid we screened and discovered the interaction of neuritin and neuralized (NEURL1), which is an important regulator that can activate Notch signaling through promoting endocytosis of Notch ligand. And then we identified the interaction of neuritin and neuralized by co-immunoprecipitation (IP) assays, and clarified that neuritin and NEURL1 were co-localized on the cell membrane of SH-SY5Y cells...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28635367/in-vivo-and-in-vitro-depolymerizations-of-intracellular-medium-chain-length-poly-3-hydroxyalkanoates-produced-by-pseudomonas-putida-bet001
#2
Siti Nor Syairah Anis, Mohamad Suffian Mohamad Annuar, Khanom Simarani
In vivo and in vitro depolymerizations of intracellular medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 grown on lauric acid was studied. Both processes were studied under optimum conditions for mcl-PHA depolymerization viz. 0.2 M Tris-HCl buffer, pH 9, ionic strength (I) = 0.2 M at 30 °C. For in vitro depolymerization studies, cell-free system was obtained from lysing bacterial cells suspension by ultrasonication at optimum conditions (frequency 37 kHz, 30% of power output, <25 (°)C for 120 minutes)...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28631597/colwellia-agarivorans-sp-nov-an-agar-digesting-marine-bacterium-isolated-from-coastal-seawater
#3
Zhen-Xing Xu, Heng-Xi Zhang, Ji-Ru Han, Christopher A Dunlap, Alejandro P Rooney, Da-Shuai Mu, Zong-Jun Du
A novel Gram-stain-negative, facultatively anaerobic, yellowish and agar-digesting marine bacterium, designated strain QM50T, was isolated from coastal seawater in an aquaculture site near Qingdao, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represented a member of the genus Colwellia and exhibited the highest sequence similarity (97.4 %) to Colwellia aestuarii SMK-10T. Average nucleotide identity (ANI) values based on draft genome sequences between strain QM50T and C...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28631050/analgesia-nociception-index-ani-monitoring-in-patients-with-thoracic-paravertebral-block-a-randomized-controlled-study
#4
Nurseda Dundar, Alparslan Kus, Yavuz Gurkan, Kamil Toker, Mine Solak
The goal of the study was to evaluate the effectiveness of analgesia nociception index (ANI) monitoring during intraoperative period for patients with thoracic paravertebral block (TPVB) undergoing breast surgery under general anesthesia. This prospective randomized trial was performed after receiving ethics committee approval in 44 patients who were scheduled to undergo breast surgery under general anesthesia. TPVB was performed in the preoperative period using 20 mL of bupivacaine 0.25% at T4 level. Anesthesia maintenance was provided with sevoflurane in O2: air mixture and remifentanil infusion...
June 19, 2017: Journal of Clinical Monitoring and Computing
https://www.readbyqxmd.com/read/28629508/campylobacter-pinnipediorum-sp-nov-isolated-from-pinnipeds-comprising-campylobacter-pinnipediorum-subsp-pinnipediorum-subsp-nov-and-campylobacter-pinnipediorum-subsp-caledonicus-subsp-nov
#5
Maarten J Gilbert, William G Miller, Judy St Leger, Mary H Chapman, Arjen J Timmerman, Birgitta Duim, Geoffrey Foster, Jaap A Wagenaar
During independent diagnostic screenings of otariid seals in California (USA) and phocid seals in Scotland (UK), Campylobacter-like isolates, which differed from the established taxa of the genus Campylobacter, were cultured from abscesses and internal organs of different seal species. A polyphasic study was undertaken to determine the taxonomic position of these six isolates. The isolates were characterized by 16S rRNA gene and AtpA sequence analysis and by conventional phenotypic testing. The whole-genome sequences were determined for all isolates, and the average nucleotide identity (ANI) was determined...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28629499/agrobacterium-salinitolerans-sp-nov-a-saline-alkaline-tolerant-bacterium-isolated-from-root-nodule-of-sesbania-cannabina
#6
Jun Yan, Yan Li, Hui Yan, Wen Feng Chen, Xiaoxia Zhang, En Tao Wang, Xiao Zeng Han, Zhi Hong Xie
Two Gram-staining-negative, aerobic bacteria (YIC 5082T and YIC4104) isolated from root nodules of Sesbania cannabina grown in a high-salt and alkaline environment were identified as a group in the genus Agrobacterium because they shared 100 and 99.7 % sequence similarities of 16S rRNA and recA+atpD genes, respectively. These two strains showed 99.2/100 % and 93.9/95.4 % 16S rRNA and recA+atpD gene sequence similarities to Agrobacterium radiobacter LMG140T and Agrobacterium. pusense NRCPB10T, respectively...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28629051/novel-biomimetic-composite-material-for-potentiometric-screening-of-acetylcholine-a-neurotransmitter-in-alzheimer-s-disease
#7
Ana S Sacramento, Felismina T C Moreira, Joana L Guerreiro, Ana P Tavares, M Goreti F Sales
This work describes a novel approach to produce an antibody-like biomimetic material. It includes preparing composite imprinted material never presented before, with highly conductive support nanostructures and assembling a high conductivity polymeric layer at low temperature. Overall, such highly conductive material may enhance the final features of electrically-based devices. Acetylcholine (ACh) was selected as target analyte, a neurotransmitter of importance in Alzheimer's disease. Potentiometric transduction was preferred, allowing quick responses and future adaptation to point-of-care requirements...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28628605/corrigendum-reevaluation-of-performance-of-electric-double-layer-capacitors-from-constant-current-charge-discharge-and-cyclic-voltammetry
#8
Anis Allagui, Todd J Freeborn, Ahmed S Elwakil, Brent J Maundy
This corrects the article DOI: 10.1038/srep38568.
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28627467/time-to-review-the-gold-standard-for-genotyping-vancomycin-resistant-enterococci-in-epidemiology-comparing-whole-genome-sequencing-with-pfge-and-mlst-in-three-suspected-outbreaks-in-sweden-during-2013-2015
#9
Birgitta Lytsy, Lars Engstrand, Åke Gustafsson, Rene Kaden
Vancomycin-resistant enterococci (VRE) are a challenge to the health-care system regarding transmission rate and treatment of infections. VRE outbreaks have to be controlled from the first cases which means that appropriate and sensitive genotyping methods are needed. The aim of this study was to investigate the applicability of whole genome sequencing based analysis compared to Pulsed-Field Gel Electrophoresis (PFGE) and Multi-Locus Sequence Typing (MLST) in epidemiological investigations as well as the development of a user friendly method for daily laboratory use...
June 13, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28626237/cd4-t-cells-memorize-obesity-and-promote-weight-regain
#10
Jianghuan Zou, Beibei Lai, Mingzhu Zheng, Qin Chen, Shujun Jiang, Anying Song, Zan Huang, Peiliang Shi, Xin Tu, Di Wang, Linrong Lu, Zhaoyu Lin, Xiang Gao
Body weight regain often causes failure of obesity therapies while the underlying mechanism remains largely unknown. In this study, we report that immune cells, especially CD4+ T cells, mediate the 'memory' of previous obese status. In a weight gain-loss-regain model, we found that C57BL/6J mice with an obesity history showed a much faster rate of body weight regain. This obesity memory could last for at least 2 months after previously obese mice were kept at the same body weight as non-obese mice. Surprisingly, such obesity memory was abrogated by dexamethasone treatment, whereas immunodeficient Rag1(-/-) and H2A(-/-) mice failed to establish such memory...
June 19, 2017: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/28625954/attenuation-of-cadmium-induced-decline-in-spatial-habituation-and-recognition-memory-by-long-term-administration-of-almond-and-walnut-supplementation-role-of-cholinergic-function
#11
Zehra Batool, Faiza Agha, Saara Ahmad, Laraib Liaquat, Saiqa Tabassum, Saima Khaliq, Lubna Anis, Irfan Sajid, Shaista Emad, Tahira Perveen, Saida Haider
Excessive exposure of cadmium which is regarded as a neurotoxin can stimulate aging process by inducing abnormality in neuronal function. It has been reported that supplementation of almond and walnut attenuate age-related memory loss. Present study was designed to investigate the weekly administration of cadmium for one month on learning and memory function with relation to cholinergic activity. Cadmium was administered at the dose of 50 mg/kg/week. Whereas, almond and walnut was supplemented at the dose of 400 mg/kg/day along with cadmium administration to separate set of rats...
January 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28625171/the-association-between-adolescent-psychopathology-and-subsequent-physical-activity-in-young-adulthood-a-21-year-birth-cohort-study
#12
S Suetani, A Mamun, G M Williams, J M Najman, J J McGrath, J G Scott
BACKGROUND: The beneficial effects of physical activity (PA) for both physical and mental wellbeing are well established. Given that adolescence presents a critical developmental period during which life-long patterns of PA become established, the exploration of the longitudinal impact of adolescent psychopathology on adult PA status is of interest. METHODS: We analysed prospective data from 3663 young adults who participated in the Mater-University of Queensland Study of Pregnancy...
June 19, 2017: Psychological Medicine
https://www.readbyqxmd.com/read/28624916/interethnic-variation-in-pelvic-floor-morphology-in-women-with-symptomatic-pelvic-organ-prolapse
#13
Zeelha Abdool, Hans Peter Dietz, Barend Gerhardus Lindeque
INTRODUCTION AND HYPOTHESIS: There is a lack of epidemiological studies evaluating female pelvic organ prolapse in developing countries. Current studies have largely focused on women of white ethnicity. This study was designed to determine interethnic variation in pelvic floor functional anatomy, namely, levator hiatal distensibility and pelvic organ descent, in women with symptomatic pelvic organ prolapse in a multi-ethnic South African population. METHODS: This prospective observational study included 258 consecutive women referred for pelvic organ prolapse assessment and management at a tertiary urogynaecological clinic...
June 17, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28623871/simple-computing-of-the-viscosity-of-water-dioxane-mixtures-according-to-a-fluctuating-spc-e-ih-interstitial-model
#14
Anis Ghazouani, Jalel M'halla
In this contribution, we propose a new simple and fast computing approach to model liquid water and water-1,4 dioxane mixture, which is intermediate between molecular dynamics calculation and lattice fluid H-bonding theory in which water molecules are arranged according to a fluctuating hexagonal Ih network. It generalizes the interstitial mixture models of Samoilov and Pauling by increasing the number of spatial configurations into the space of phases due to the addition of rotations and vibrations of the molecules around their equilibrium positions...
June 17, 2017: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/28620795/levator-ani-defects-and-the-severity-of-symptoms-in-women-with-anterior-compartment-pelvic-organ-prolapse
#15
Sissel H Oversand, Anne C Staff, Leiv Sandvik, Ingrid Volløyhaug, Rune Svenningsen
INTRODUCTION AND HYPOTHESIS: The aims of this study were to evaluate the prevalence of levator ani muscle (LAM) avulsions in a selected cohort of patients with primary anterior compartment pelvic organ prolapse (POP) and to assess whether LAM avulsions, as an independent factor, affect the degree of POP symptoms and sexual dysfunction. Additionally, clinical and demographic variables of women with and those without avulsions were compared. METHODS: We carried out a cross-sectional analysis of a prospective cohort study including 197 women scheduled for anterior compartment POP surgery...
June 15, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28614294/human-fetal-dendritic-cells-promote-prenatal-t-cell-immune-suppression-through-arginase-2
#16
Naomi McGovern, Amanda Shin, Gillian Low, Donovan Low, Kaibo Duan, Leong Jing Yao, Rasha Msallam, Ivy Low, Nurhidaya Binte Shadan, Hermi R Sumatoh, Erin Soon, Josephine Lum, Esther Mok, Sandra Hubert, Peter See, Edwin Huang Kunxiang, Yie Hou Lee, Baptiste Janela, Mahesh Choolani, Citra Nurfarah Zaini Mattar, Yiping Fan, Tony Kiat Hon Lim, Dedrick Kok Hong, Ker-Kan Tan, John Kit Chung Tam, Christopher Schuster, Adelheid Elbe-Bürger, Xiao-Nong Wang, Venetia Bigley, Matthew Collin, Muzlifah Haniffa, Andreas Schlitzer, Michael Poidinger, Salvatore Albani, Anis Larbi, Evan W Newell, Jerry Kok Yen Chan, Florent Ginhoux
During gestation the developing human fetus is exposed to a diverse range of potentially immune-stimulatory molecules including semi-allogeneic antigens from maternal cells, substances from ingested amniotic fluid, food antigens, and microbes. Yet the capacity of the fetal immune system, including antigen-presenting cells, to detect and respond to such stimuli remains unclear. In particular, dendritic cells, which are crucial for effective immunity and tolerance, remain poorly characterized in the developing fetus...
June 14, 2017: Nature
https://www.readbyqxmd.com/read/28611492/genome-mining-and-predictive-functional-profiling-of-acidophilic-rhizobacterium-pseudomonas-fluorescens-pt14
#17
Pooja Rani, Nitish Kumar Mahato, Anukriti Sharma, Desiraju Lakshmi Narsimha Rao, Komal Kamra, Rup Lal
Pseudomonas fluorescens Pt14 is a non-pathogenic and acidophilic bacterium isolated from acidic soil (pH 4.65). Genome sequencing of strain Pt14 was performed using Single Molecule Real Time (SMRT) sequencing to get insights into unique existence of this strain in acidic environment. Complete genome sequence of this strain revealed a chromosome of 5,841,722 bp having 5354 CDSs and 88 RNAs. Phylogenomic reconstruction based on 16S rRNA gene, Average Nucleotide Identity (ANI) values and marker proteins revealed that strain Pt14 shared a common clade with P...
June 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28608316/winogradskyella-aurantia-sp-nov-isolated-from-a-marine-solar-saltern
#18
Yue Sun, Bi-Yao Chen, Zong-Jun Du
A Gram-stain negative, non-flagellated, facultatively anaerobic, slightly halophilic bacterium, WNB302(T), was isolated from a marine solar saltern in Wendeng, China (122°0'38.85″E, 36°57'56.49″N). Cells of strain WNB302(T) were 0.2-0.7 µm wide and 2.0-10.0 µm long, catalase- positive and oxidase-negative. Colonies were opaque, orange and approximately 1.0-2.0 mm in diameter after culture for 96 h on marine agar 2216. Growth occurs at 15-37 °C (optimum, 33-35 °C), pH 6.0-8.5 (optimum, pH 7.0-7...
June 12, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28601967/bradyrhizobium-sacchari-sp-nov-a-legume-nodulating-bacterium-isolated-from-sugarcane-roots
#19
Gustavo Feitosa de Matos, Jerri Edson Zilli, Jean Luiz Simões de Araújo, Marcia Maria Parma, Itamar Soares Melo, Viviane Radl, José Ivo Baldani, Luc Felicianus Marie Rouws
Members of the genus Bradyrhizobium are well-known as nitrogen-fixing microsymbionts of a wide variety of leguminous species, but they have also been found in different environments, notably as endophytes in non-legumes such as sugarcane. This study presents a detailed polyphasic characterization of four Bradyrhizobium strains (type strain BR 10280(T)), previously isolated from roots of sugarcane in Brazil. 16S rRNA sequence analysis, multilocus sequence analysis (MLSA) and analysis of the 16S-23S rRNA internal transcribed spacer showed that these strains form a novel clade close to, but different from B...
June 10, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28600685/aquidulcibacter-paucihalophilus-gen-nov-sp-nov-a-novel-member-of-family-caulobacteraceae-isolated-from-cyanobacterial-aggregates-in-a-eutrophic-lake
#20
Haiyuan Cai, Yuan Shi, Yanan Wang, Henglin Cui, Helong Jiang
A bacterial strain, TH1-2(T), was isolated from cyanobacterial aggregates in the eutrophic Lake Taihu, Jiangsu Province, China. Cells were observed to be Gram-negative, slightly curved and rod-shaped. Optimal growth was obtained at pH 7.0 (range 5.5-8.5) and 30 °C (range 20-37 °C) in trypticase soy broth (TSB) without NaCl. Growth was not observed in TSB with 1.0% (w/v) NaCl added. The cells were found to be positive for oxidase and catalase activities. The major fatty acids were identified as 3-hydroxy hexadecanoic acid (C16:0 3-OH), C16:1 ω5c and summed feature 8 (consisting of C18:1 ω6c and cis-11-Octadecenoic acid (C18:1 ω7c)...
June 9, 2017: Antonie Van Leeuwenhoek
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