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https://www.readbyqxmd.com/read/28729266/draft-genome-sequence-of-streptomyces-sp-m1013-a-close-relative-of-streptomyces-ambofaciens-and-streptomyces-coelicolor
#1
Drago Haas, Claude Gerbaud, Nevzat Sahin, Jean-Luc Pernodet, Sylvie Lautru
We report the draft genome sequence of Streptomyces sp. M1013, a strain isolated from the Medicago arborea rhizosphere in Izmir, Turkey. An average nucleotide identity (ANI) analysis reveals that this strain belongs to the same species as Streptomyces canus ATCC12647 and is closely related to Streptomyces ambofaciens and Streptomyces coelicolor.
July 20, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28727287/weighing-the-risks-morbid-obesity-and-diabetes-are-associated-with-increased-risk-of-death-on-the-liver-transplant-waiting-list
#2
Ani A Kardashian, Jennifer L Dodge, John Roberts, Danielle Brandman
Obesity is a growing problem in liver transplant (LT) candidates, paralleling the U.S. obesity epidemic and increase in LT for nonalcoholic steatohepatitis (NASH). While post-LT survival appears to be similar in obese and non-obese patients, data is scarce regarding risk of waitlist dropout in patients with morbid obesity (BMI≥40 kg/m(2) ). We examined the impact of obesity on waitlist mortality and evaluated predictors of dropout in LT candidates with morbid obesity or NASH METHODS: Competing risk analyses were performed in candidates listed between 3/2002-12/2013 to evaluate predictors of waitlist removal or death...
July 20, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/28725436/factors-associated-with-increased-pancreatic-enzymes-in-septic-patients-a-prospective-study
#3
Anis Chaari, Karim Abdel Hakim, Nevine Rashed, Kamel Bousselmi, Vipin Kauts, Mahmoud Etman, William Francis Casey
BACKGROUND: The perfusion of splanchnic organs is deeply altered in patients with septic shock. The aim of the study is to identify the predictive factors of septic shock-induced increase of serum lipase and amylase and to assess and evaluate its prognostic impact. METHODS: We conducted a prospective observational study. All adult patients admitted with septic shock were eligible for our study. Serum lipase and amylase were measured on admission. Patients with and those without increased pancreatic enzymes were compared...
2017: Journal of Intensive Care
https://www.readbyqxmd.com/read/28724804/pediatric-dilated-cardiomyopathy-hearts-display-a-unique-gene-expression-profile
#4
Philip D Tatman, Kathleen C Woulfe, Anis Karimpour-Fard, Danielle A Jeffrey, James Jaggers, Joseph C Cleveland, Karin Nunley, Matthew Rg Taylor, Shelley D Miyamoto, Brian L Stauffer, Carmen C Sucharov
Our previous work showed myocellular differences in pediatric and adult dilated cardiomyopathy (DCM). However, a thorough characterization of the molecular pathways involved in pediatric DCM does not exist, limiting the development of age-specific therapies. To characterize this patient population, we investigated the transcriptome profile of pediatric patients. RNA-Seq from 7 DCM and 7 nonfailing (NF) explanted age-matched pediatric left ventricles (LV) was performed. Changes in gene expression were confirmed by real-time PCR (RT-PCR) in 36 DCM and 21 NF pediatric hearts and in 20 DCM and 10 NF adult hearts...
July 20, 2017: JCI Insight
https://www.readbyqxmd.com/read/28723550/induced-pluripotent-stem-cell-derived-primitive-macrophages-provide-a-platform-for-modeling-tissue-resident-macrophage-differentiation-and-function
#5
Kazuyuki Takata, Tatsuya Kozaki, Christopher Zhe Wei Lee, Morgane Sonia Thion, Masayuki Otsuka, Shawn Lim, Kagistia Hana Utami, Kerem Fidan, Dong Shin Park, Benoit Malleret, Svetoslav Chakarov, Peter See, Donovan Low, Gillian Low, Marta Garcia-Miralles, Ruizhu Zeng, Jinqiu Zhang, Chi Ching Goh, Ahmet Gul, Sandra Hubert, Bernett Lee, Jinmiao Chen, Ivy Low, Nurhidaya Binte Shadan, Josephine Lum, Tay Seok Wei, Esther Mok, Shohei Kawanishi, Yoshihisa Kitamura, Anis Larbi, Michael Poidinger, Laurent Renia, Lai Guan Ng, Yochai Wolf, Steffen Jung, Tamer Önder, Evan Newell, Tara Huber, Eishi Ashihara, Sonia Garel, Mahmoud A Pouladi, Florent Ginhoux
Tissue macrophages arise during embryogenesis from yolk-sac (YS) progenitors that give rise to primitive YS macrophages. Until recently, it has been impossible to isolate or derive sufficient numbers of YS-derived macrophages for further study, but data now suggest that induced pluripotent stem cells (iPSCs) can be driven to undergo a process reminiscent of YS-hematopoiesis in vitro. We asked whether iPSC-derived primitive macrophages (iMacs) can terminally differentiate into specialized macrophages with the help of growth factors and organ-specific cues...
July 18, 2017: Immunity
https://www.readbyqxmd.com/read/28723090/transformative-heterointerface-evolution-and-plasmonic-tuning-of-anisotropic-trimetallic-nanoparticles
#6
Mouhong Lin, Gyeong-Hwan Kim, Jae-Ho Kim, Jeong-Wook Oh, Jwa-Min Nam
Multi-component nanoparticles that incorporate multiple nano-crystal domains into a single particle represent an important class of material with highly tailorable structures and properties. The controlled synthesis of multi-component NPs with 3 or more components with the desired structure, particularly aniso-tropic structures, and property is, however, highly challenging. Here, we developed a polymer and galvanic replacement reac-tion-based transformative heterointerface evolution (THE) method to form and tune gold-copper-silver multi-metallic ani-sotropic nanoparticles (MAPs) with well-defined configurations, including structural order, uniformity and junction geometry, giving rise to extraordinarily high tunability in the structural design, synthesis and optical property of tri-metallic plasmonic nanoantenna structures...
July 19, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28722334/potential-emerging-treatment-in-vitiligo-using-er-yag-in-combination-with-5fu-and-clobetasol
#7
Fatemeh Mokhtari, Anis Bostakian, Zabihollah Shahmoradi, Tohid Jafari-Koshki, Fariba Iraji, Gita Faghihi, Sayed Mohsen Hosseini, Behzad Bafandeh
BACKGROUND: Vitiligo is a pigmentary disorder of skin affecting at least 1% of the world population of all races in both sexes. Its importance is mainly due to subsequent social and psychological problems rather than clinical complications. Various treatment choices are available for vitiligo; however, laser-based courses have shown to give more acceptable results. OBJECTIVE: The aim of this trial was to evaluate the efficacy of Er:YAG laser as a supplementary medicine to topical 5FU and clobetasol in vitiligo patients...
July 19, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28720571/the-integrated-stress-response-in-hypoxia-induced-diffuse-white-matter-injury
#8
Benjamin Ll Clayton, Aaron Huang, Rejani B Kunjamma, Ani Solanki, Brian Popko
Currently no treatments exist for preterm infants with diffuse white matter injury (DWMI) caused by hypoxia. Due to improved care of preterm neonates and increased recognition by advanced imaging techniques, the prevalence of DWMI is increasing. A better understanding of the pathophysiology of DWMI is therefore of critical importance. The integrated stress response (ISR), a conserved eukaryotic response to myriad stressors including hypoxia, may play a role in hypoxia-induced DWMI and may represent a novel target for much needed therapies...
July 18, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28720568/serine-proteases-of-the-complement-lectin-pathway-and-their-genetic-variations-in-ischaemic-stroke
#9
Gohar Tsakanova, Ani Stepanyan, Karen Nahapetyan, Robert B Sim, Arsen Arakelyan, Anna Boyajyan
AIMS: The aim of the current study was to assess the proteolytic activities of collectin-bound MASP-1 and MASP-2 in the blood of patients with ischaemic stroke, as well as the association of their six genetic polymorphisms (rs3203210, rs28945070, rs28945073 in MASP1 gene and rs2273343, rs12711521, rs147270785 in MASP2 gene) with this pathology. METHODS: In total, 250 patients and 300 healthy subjects were involved in this study. MBL-associated serine protease (MASP)-1 and MASP-2 activities were measured using in-house developed immunofluorescent and enzyme-linked immunosorbent assays, respectively...
July 18, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28719704/thyrocervical-trunk-pseudoaneurysm-presenting-as-a-neck-mass
#10
Taha A Mur, Mursalin M Anis, Ahmed M Soliman
No abstract text is available yet for this article.
July 2017: Ear, Nose, & Throat Journal
https://www.readbyqxmd.com/read/28718043/impact-of-rituximab-on-patient-reported-outcomes-in-patients-with-rheumatoid-arthritis-from-the-us-corrona-registry
#11
Leslie R Harrold, Ani John, Jennie Best, Steve Zlotnick, Chitra Karki, YouFu Li, Jeffrey D Greenberg, Joel M Kremer
To evaluate the impact of rituximab on patient-reported outcomes (PROs) in a US-based observational cohort of patients with rheumatoid arthritis (RA). Patients with active RA, prior exposure to ≥1 tumor necrosis factor inhibitor (TNFi) and who newly initiated rituximab were identified. Changes in PROs were assessed 1 year after rituximab initiation. PRO measures included Clinical Disease Activity Index (CDAI); patient global disease activity, pain and fatigue (visual analog score; 0-100); morning stiffness (hours); modified Health Assessment Questionnaire (mHAQ; 0-3); and EuroQoL EQ-5D...
July 17, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28715156/neutrophil-mediated-enhancement-of-angiogenesis-and-osteogenesis-in-a-novel-triple-cell-co-culture-model-with-endothelial-cells-and-osteoblasts
#12
T D K Herath, Anis Larbi, Swee Hin Teoh, C James Kirkpatrick, Bee Tin Goh
Repair and regeneration of critical-sized bone defects remain a major challenge in orthopaedic and craniomaxillofacial surgery. Until now, attempts to bioengineer bone tissue have been hindered by the inability to establish proper angiogenesis and osteogenesis in the tissue construct. In the present study, we established a novel triple cell co-culture model consisting of osteoblasts, endothelial cells, and neutrophils and conducted a systematic investigation of the effects of neutrophils on angiogenesis and osteogenesis...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28713838/role-of-interferons-in-the-development-of-diagnostics-vaccines-and-therapy-for-tuberculosis
#13
REVIEW
Kai Ling Chin, Fadhilah Zulkipli Anis, Maria E Sarmiento, Mohd Nor Norazmi, Armando Acosta
Tuberculosis (TB) is an airborne infection caused by Mycobacterium tuberculosis (Mtb). About one-third of the world's population is latently infected with TB and 5-15% of them will develop active TB in their lifetime. It is estimated that each case of active TB may cause 10-20 new infections. Host immune response to Mtb is influenced by interferon- (IFN-) signaling pathways, particularly by type I and type II interferons (IFNs). The latter that consists of IFN-γ has been associated with the promotion of Th1 immune response which is associated with protection against TB...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28713281/effectiveness-of-traditional-strength-vs-power-training-on-muscle-strength-power-and-speed-with-youth-a-systematic-review-and-meta-analysis
#14
REVIEW
David G Behm, James D Young, Joseph H D Whitten, Jonathan C Reid, Patrick J Quigley, Jonathan Low, Yimeng Li, Camila D Lima, Daniel D Hodgson, Anis Chaouachi, Olaf Prieske, Urs Granacher
Numerous national associations and multiple reviews have documented the safety and efficacy of strength training for children and adolescents. The literature highlights the significant training-induced increases in strength associated with youth strength training. However, the effectiveness of youth strength training programs to improve power measures is not as clear. This discrepancy may be related to training and testing specificity. Most prior youth strength training programs emphasized lower intensity resistance with relatively slow movements...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28711981/universal-direct-pcr-amplification-system-a-time-and-cost-effective-tool-for-high-throughput-applications
#15
Anis Ben-Amar, Souheib Oueslati, Ahmed Mliki
Taking into account the limits of current genotyping methodologies, we have established a versatile direct PCR method on intact microtissue samples without prior DNA isolation. A simple and standard protocol was developed and validated on a wide range of living organisms including bacterial and fungal strains, plant species and human samples. This allows reliable amplification of target genomic DNA fragment directly from source material using minimal amount of tissue which makes DNA purification irrelevant for a number of biological applications...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28711559/outbreak-of-colistin-resistant-carbapenemase-producing-klebsiella-pneumoniae-in-tunisia
#16
Wejdène Mansour, Marisa Haenni, Estelle Saras, Raoudha Grami, Yosra Mani, Anis Ben Haj Khalifa, Souhail El Atrouss, Mohamed Kheder, Mohamed Fekih Hassen, Noureddine Boujâafar, Olfa Bouallegue, Jean-Yves Madec
OBJECTIVES: Mechanisms of colistin and carbapenem resistance among a collection of Klebsiella pneumoniae isolates recovered in a university hospital in Tunisia were studied. METHODS: In vitro antimicrobial susceptibility testing, S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), Southern blotting and PCR-based replicon typing (PBRT) were performed. Extended-spectrum β-lactamases (ESBLs), carbapenemases, AmpC-type enzymes and mgrB genes were detected by PCR and sequencing...
July 12, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28710417/talen-based-hpv-e7-editing-triggers-necrotic-cell-death-in-cervical-cancer-cells
#17
Sumitra Shankar, Deepti Prasad, Rahul Sanawar, Ani V Das, M Radhakrishna Pillai
Human Papillomavirus E7 and E6 oncoproteins have been considered as suitable candidate anti-viral targets since they cause malignant conversion in cervical cancers. Transcription Activator-Like Effector Nucleases (TALENs) are recent editing tools to knockout genes by inducing double stranded breaks at specific sites in the genome. In here, we have designed specific TALENs to target E7 and analyzed their efficiency in inducing cell death in cervical cancer cells. We found that designed TALENs could yield about 10-12% editing activity as observed from T7E1 and nuclease resistance assays...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28709583/interactions-between-pelvic-organ-protrusion-levator-ani-descent-and-hiatal-enlargement-in-women-with-and-without-prolapse
#18
Anne G Sammarco, Lahari Nandikanti, Emily K Kobernik, Bing Xie, Alexandra Jankowski, Carolyn W Swenson, John O L Delancey
BACKGROUND: Pelvic organ prolapse has two components; 1) protrusion of the pelvic organs beyond the hymen and 2) descent of the levator ani. The Pelvic Organ Prolapse Quantification system measures the first component, however, there remains no standard measurement protocol for the second mechanism. OBJECTIVES: Test the hypotheses that 1) difference in the protrusion area is greater than the area created by levator descent in prolapse patients compared with controls and, 2) Prolapse is more strongly associated with levator hiatus compared to urogenital hiatus...
July 11, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28709483/what-remains-to-surgeons-in-the-management-of-abdominal-tuberculosis-a-10-years-experience-in-an-endemic-area
#19
Rabii Noomene, Asma Ouakaa, Raja Jouini, Anis Ben Maamer, Abderraouf Cherif
BACKGROUND: Tuberculosis (TB) is a common endemic disease in Tunisia. Abdominal location is rare. Early diagnosis of abdominal TB remains difficult due to its non-specific clinical presentations. The aim of our study is to highlight the characteristics of the different presentations, to characterize tools contributing to a positive preoperative diagnosis, and finally to assess the role of surgery in the management of this entity. MATERIALS AND METHODS: A retrospective review from 2005 to 2015 identified 90 cases of confirmed abdominal TB managed in the Department of General Surgery of the Habib Thameur Hospital...
July 2017: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/28708057/erythrobacter-xanthus-sp-nov-isolated-from-surface-seawater-of-the-south-china-sea
#20
Dan-Dan Li, Yan-Qi Zhang, Ming Peng, Ning Wang, Xiu-Juan Wang, Xi-Ying Zhang, Ping-Yi Li, Bin-Bin Xie, Xiu-Lan Chen, Yu-Zhong Zhang, Qi-Long Qin
A Gram-reaction-negative, aerobic, oxidase- and catalase-positive, yellow-pigmented, non-flagellated, rod-shaped bacterium, designed strain SM1501T, was isolated from surface seawater of the South China Sea. SM1501T grew at 7-42 °C and with 0-11 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that SM1501T represented a member of the genus Erythrobacter, sharing the highest 16S rRNA gene sequence similarity (97.4 %) with Erythrobacter luteus and 94.2-96.5 % 16S rRNA gene sequence similarities to other species of the genus Erythrobacterwith validly published names...
July 15, 2017: International Journal of Systematic and Evolutionary Microbiology
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