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https://www.readbyqxmd.com/read/29713278/dehydroepiandrosterone-antagonizes-pain-stress-induced-suppression-of-testosterone-production-in-male-rats
#1
Qiqi Zhu, Fei Ge, Xiaoheng Li, Hou-Sheng Deng, Miao Xu, Tiao Bu, Jingyang Li, Yiyan Wang, Yuanyuan Shan, Ren-Shan Ge, Ming Yao
Background: Leydig cells secrete the steroid hormone, testosterone, which is essential for male fertility and reproductive health. Stress increases the secretion of glucocorticoid [corticosterone, (CORT) in rats] that decreases circulating testosterone levels in part through a direct action on its receptors in Leydig cells. Intratesticular CORT level is dependent on oxidative inactivation of CORT by 11β-hydroxysteroid dehydrogenase 1 (HSD11B1) in rat Leydig cells. Pain may cause the stress, thus affecting testosterone production in Leydig cells...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29699561/docosahexaenoic-acid-dha-effects-on-proliferation-and-steroidogenesis-of-bovine-granulosa-cells
#2
Virginie Maillard, Alice Desmarchais, Maeva Durcin, Svetlana Uzbekova, Sebastien Elis
BACKGROUND: Docosahexaenoic acid (DHA) is a n-3 polyunsaturated fatty acid (PUFA) belonging to a family of biologically active fatty acids (FA), which are known to have numerous health benefits. N-3 PUFAs affect reproduction in cattle, and notably directly affect follicular cells. In terms of reproduction in cattle, n-3 PUFA-enriched diets lead to increased follicle size or numbers. METHODS: The objective of the present study was to analyze the effects of DHA (1, 10, 20 and 50 μM) on proliferation and steroidogenesis (parametric and/or non parametric (permutational) ANOVA) of bovine granulosa cells in vitro and mechanisms of action through protein expression (Kruskal-Wallis) and signaling pathways (non parametric ANOVA) and to investigate whether DHA could exert part of its action through the free fatty acid receptor 4 (FFAR4)...
April 26, 2018: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29674118/germline-variant-in-hsd3b1-1245-a-c-and-response-to-abiraterone-acetate-plus-prednisone-in-men-with-new-onset-metastatic-castration-resistant-prostate-cancer
#3
Andrew W Hahn, David M Gill, Roberto H Nussenzveig, Austin Poole, Jim Farnham, Lisa Cannon-Albright, Neeraj Agarwal
BACKGROUND: The HSD3B1 gene encodes the enzyme 3β-hydroxysteroid dehydrogenase-1 (3βHSD1), which catalyzes adrenal androgen precursors into dihydrotestosterone, the most potent androgen. Recently, the HSD3B1 (1245C) variant was shown to predict shorter duration of response to androgen deprivation therapy with medical or surgical castration in the setting of castration-sensitive prostate cancer (CSPC). The HSD3B1 (1245C) variant also predicts longer duration of response to ketoconazole in men with castration-resistant prostate cancer (CRPC)...
March 27, 2018: Clinical Genitourinary Cancer
https://www.readbyqxmd.com/read/29669061/zearalenone-delays-rat-leydig-cell-regeneration
#4
Songyi Zhou, Yiyan Wang, Leikai Ma, Xianwu Chen, Yao Lü, Fei Ge, Yong Chen, Xiaofang Chen, Qingquan Lian, Xiao-Dong Jin, Ren-Shan Ge
Zearalenone (ZEA), a fungal mycotoxin, is present in a wide range of human foods. By virtual screening, we have identified that ZEA is a potential endocrine disruptor of Leydig cells. The effect of ZEA on Leydig cell development is still unclear. The objective of the present study was to explore whether ZEA affected Leydig cell developmental process and to clarify the underlying mechanism. Adult male Sprague Dawley rats (60 days old) were randomly divided into three groups and these rats received a single intraperitoneal injection of 75 mg/kg ethane dimethane sulfonate (EDS) to eliminate all Leydig cells...
April 16, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29667777/induced-pluripotent-stem-cell-derived-conditional-medium-promotes-leydig-cell-anti-apoptosis-and-proliferation-via-autophagy-and-wnt-%C3%AE-catenin-pathway
#5
Xiaoling Guo, Yong Chen, Tingting Hong, Xianwu Chen, Yue Duan, Chao Li, Renshan Ge
Leydig cell transplantation is a better alternative in the treatment of androgen-deficient males. The main purpose of this study was to investigate the effects of induced pluripotent stem cell-derived conditioned medium (iPS-CM) on the anti-apoptosis, proliferation and function of immature Leydig cells (ILCs), and illuminate the underlying mechanisms. ILCs were exposed to 200 μmol/L hydrogen peroxide (H2 O2 ) for 24 hours with or without iPS-CM treatments. Cell apoptosis was detected by flow cytometric analysis...
April 18, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29613814/grape-seed-procyanidins-extract-attenuates-cisplatin-induced-oxidative-stress-and-testosterone-synthase-inhibition-in-rat-testes
#6
Minmin Tian, Fangfang Liu, Han Liu, Qiong Zhang, Lei Li, Xiangbo Hou, Jianxin Zhao, Sheng Li, Xuhong Chang, Yingbiao Sun
Cisplatin (CIS) is widely applied for its antihematological malignancies properties and as antisolid tumors drugs. However, it could cause testicular damage related with oxidative stress and testosterone synthesis disorder. Studies reported that grape seed procyanidins extract (GSPE) could improve CIS induced-testes lesion via scavenging free radicals in animals, although its mechanisms were unclear. Therefore, the purpose of the present study was to explore the antagonistic mechanisms of GSPE on CIS-induced testes lesion...
April 3, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29588428/expression-of-sult2a1-sult2b1-and-hsd3b1-in-the-porcine-testis-and-epididymis
#7
Bettina Zimmer, Linda Tenbusch, Michele C Klymiuk, Yaser Dezhkam, Gerhard Schuler
In the porcine testis in addition to estrogen sulfates the formation of numerous sulfonated neutral hydroxysteroids has been observed. However, their functions and the underlying synthetic pathways are still widely unclear. To obtain further information on their formation in postpubertal boars the expression of sulfotransferases considered relevant for neutral hydroxysteroids (SULT2A1, SULT2B1) was investigated in the testis and defined segments of the epididymis applying real-time RT-qPCR, Western blot and immunohistochemistry (IHC)...
March 27, 2018: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/29540470/sprouty2-loss-induced-il6-drives-castration-resistant-prostate-cancer-through-scavenger-receptor-b1
#8
Rachana Patel, Janis Fleming, Ernest Mui, Carolyn Loveridge, Peter Repiscak, Arnaud Blomme, Victoria Harle, Mark Salji, Imran Ahmad, Katy Teo, Freddie C Hamdy, Ann Hedley, Niels van den Broek, Gillian Mackay, Joanne Edwards, Owen J Sansom, Hing Y Leung
Metastatic castration-resistant prostate cancer (mCRPC) is a lethal form of treatment-resistant prostate cancer and poses significant therapeutic challenges. Deregulated receptor tyrosine kinase (RTK) signalling mediated by loss of tumour suppressor Sprouty2 (SPRY2) is associated with treatment resistance. Using pre-clinical human and murine mCRPC models, we show that SPRY2 deficiency leads to an androgen self-sufficient form of CRPC Mechanistically, HER2-IL6 signalling axis enhances the expression of androgen biosynthetic enzyme HSD3B1 and increases SRB1-mediated cholesterol uptake in SPRY2-deficient tumours...
March 14, 2018: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/29534958/star-protein-and-steroidogenic-enzyme-expressions-in-the-rat-harderian-gland
#9
Sara Falvo, Gabriella Chieffi Baccaria, Giuseppe Spaziano, Luigi Rosati, Massimo Venditti, Maria Maddalena Di Flore, Alessandra Santillo
The Harderian gland (HG) of the rat (Rattus norvegicus) secretes copious amounts of lipids, such as cholesterol. Here we report a study of the expressions of the StAR protein and key steroidogenic enzymes in the HG of male and female rats. The objective of the present investigation was to ascertain (a) whether the rat HG is involved in steroid production starting with cholesterol, and (b) whether the pattern of gene and protein expressions together with the enzymatic activities display sexual dimorphism. The results demonstrate, for the first time, the expression of StAR gene and protein, and Cyp11a1, Hsd3b1, Hsd17b3, Srd5a1, Srd5a2 and Cyp19a1 genes in the rat HG...
March 10, 2018: Comptes Rendus Biologies
https://www.readbyqxmd.com/read/29524480/bisphenol-a-stimulates-differentiation-of-rat-stem-leydig-cells-in-vivo-and-in-vitro
#10
Lanlan Chen, Yu Zhao, Linchao Li, Lubin Xie, Xianwu Chen, Jianpeng Liu, Xiaoheng Li, Lixu Jin, Xingwang Li, Ren-Shan Ge
Bisphenol A (BPA) is widely used in consumer products and a potential endocrine disruptor linked with sexual precocity. However, its action and underlying mechanisms on male sexual maturation is unclear. In the present study, we used a unique in vivo ethane dimethane sulfonate (EDS)-induced Leydig cell regeneration model that mimics the pubertal development of Leydig cells and an in vitro stem Leydig cell differentiation model to examine the roles of BPA in Leydig cell development in rats. Intratesticular exposure to doses (100 and 1000 pmol/testis) of BPA from post-EDS day 14-28 stimulated Leydig cell developmental regeneration process by increasing serum testosterone level and Leydig cell-specific gene (Lhcgr, Star, Cyp11a1, Hsd3b1, Cyp17a1, Hsd17b3, and Hsd11b1) and their protein expression levels...
March 7, 2018: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29521016/dullard-deficiency-causes-hemorrhage-in-the-adult-ovarian-follicles
#11
Tadayoshi Hayata, Masahiko Chiga, Yoichi Ezura, Makoto Asashima, Hidetaka Katabuchi, Ryuichi Nishinakamura, Masaki Noda
In mammals, the ovarian follicles are regulated at least in part by bone morphogenetic protein (BMP) family members. Dullard (also known as Ctdnep1) gene encodes a phosphatase that suppresses BMP signaling by inactivating or degrading BMP receptors. Here we report that the Col1a1-Cre-induced Dullard mutant mice displayed hemorrhagic ovarian cysts, with red blood cells accumulated in the follicles, resulting in infertility. Cells expressing Cre driven by Col1a1 2.3-kb promoter and their descendants were found in granulosa cells in the ovary and in Sertoli cells in the testis...
May 2018: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/29514442/effects-of-nandrolone-decanoate-on-expression-of-steroidogenic-enzymes-in-the-rat-testis
#12
TaeSun Min, Ki-Ho Lee
OBJECTIVE: Nandrolone decanoate (ND) is an anabolic-androgenic steroid frequently used for clinical treatment. However, the inappropriate use of ND results in the reduction of serum testosterone level and sperm production. The suppressive effect of ND on testosterone production has not been investigated in detail. The present study was designed to examine the effect of ND on the expression of steroidogenic enzymes in the rat testis. METHODS: Male Sprague Dawley rats at 50 days of age were subcutaneously administrated with either 2 or 10 mg of ND/kg body weight/week for 2 or 12 weeks...
May 2018: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/29486070/expression-and-activity-profiling-of-the-steroidogenic-enzymes-of-glucocorticoid-biosynthesis-and-the-fdx1-co-factors-in-zebrafish
#13
M Weger, N Diotel, B D Weger, T Beil, A Zaucker, H L Eachus, J A Oakes, J L do Rego, K-H Storbeck, P Gut, U Strähle, S Rastegar, F Müller, N Krone
The spatial and temporal expression of steroidogenic genes in zebrafish has not been fully characterised. Because zebrafish are increasingly employed in endocrine and stress research, a better characterisation of steroidogenic pathways is required to target specific steps in the biosynthetic pathways. In the present study, we have systematically defined the temporal and spatial expression of steroidogenic enzymes involved in glucocorticoid biosynthesis (cyp21a2, cyp11c1, cyp11a1, cyp11a2, cyp17a1, cyp17a2, hsd3b1, hsd3b2), as well as the mitochondrial electron-providing ferredoxin co-factors (fdx1, fdx1b), during zebrafish development...
April 2018: Journal of Neuroendocrinology
https://www.readbyqxmd.com/read/29435998/environmental-mixture-with-estrogenic-activity-increases-hsd3b1-expression-through-estrogen-receptors-in-immature-rat-granulosa-cells
#14
Dragana Samardzija Nenadov, Kristina Pogrmic-Majkic, Svetlana Fa, Bojana Stanic, Aleksandra Tubic, Nebojsa Andric
Humans are exposed not only to single endocrine disruptors, but also to chemical mixtures that can adversely affect their reproductive health. Steroidogenesis in reproductive tissues is emerging as the key target of endocrine disruptor action. Here, we analyzed the effect of environmental chemical mixtures with estrogenic activity on steroidogenic processes in immature rat granulosa cells and whether the observed steroidogenic effects were mediated through estrogen receptors. Extracts from untreated wastewater were prepared by solid-phase extraction and silica gel fractionation...
June 2018: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29304256/dynamics-ultrastructure-and-gene-expression-of-human-in-vitro-organized-testis-cells-from-testicular-sperm-extraction-biopsies
#15
Kathrein von Kopylow, Wolfgang Schulze, Andrea Salzbrunn, Matthias Schaks, Elke Schäfer, Beate Roth, Stefan Schlatt, Andrej-Nikolai Spiess
STUDY QUESTION: Is it possible to induce in vitro reorganization of primary human testis cells from testicular sperm extraction (TESE) biopsies, maintain their long-term cultivation in a 2D system and identify cellular compositions? SUMMARY ANSWER: In vitro reorganization of primary human testis cells from TESE biopsies and their long-term cultivation on uncoated cell culture dishes is feasible and the cellular compositions can be uncovered through gene expression and microscopic analyses...
March 1, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29231195/hsd3b1-status-as-a-biomarker-of-androgen-deprivation-resistance-and-implications-for-prostate-cancer
#16
REVIEW
Daniel Hettel, Nima Sharifi
Patients with advanced prostate cancer who receive androgen deprivation therapy (ADT) almost invariably develop castration-resistant disease. The mechanism of resistance is largely based on synthesis of intratumoral androgens from adrenal precursors, requiring enzymatic action of 3β-hydroxysteroid dehydrogenase/Δ5→4 isomerase 1 (3β-HSD1), encoded by HSD3B1. A nucleotide polymorphism (1245A>C) in HSD3B1 results in a protein variant with increased steady-state levels and subsequently increased androgen synthesis from extragonadal precursors...
March 2018: Nature Reviews. Urology
https://www.readbyqxmd.com/read/29197250/in-utero-single-low-dose-exposure-of-cadmium-induces-rat-fetal-leydig-cell-dysfunction
#17
Xiaojun Li, Jianpeng Liu, Siwen Wu, Wenwen Zheng, Huitao Li, Suhao Bao, Yong Chen, Xiaoling Guo, Lei Zhang, Ren-Shan Ge
Cadmium chloride (Cd) is a potent endocrine disruptor and may cause the malformation in the male reproductive tract. However, the effects of a single in utero exposure to low doses of Cd on fetal Leydig cell development are still unknown. The objective of this study is to investigate the effects of a single in utero exposure to low doses of Cd on rat fetal Leydig cell development. Adult 64-day-old Sprague-Dawley dams received a single intraperitoneal injection of 0, 0.25, 0.5, and 1.0 mg/kg Cd on gestational day 12...
March 2018: Chemosphere
https://www.readbyqxmd.com/read/29133938/prostate-cancer-response-prediction-with-hsd3b1
#18
Clemens Thoma
No abstract text is available yet for this article.
December 2017: Nature Reviews. Urology
https://www.readbyqxmd.com/read/29095916/transcriptomic-difference-in-bovine-blastocysts-following-vitrification-and-slow-freezing-at-morula-stage
#19
Alisha Gupta, Jaswant Singh, Isabelle Dufort, Claude Robert, Fernanda Caminha Faustino Dias, Muhammad Anzar
Cryopreservation is known for its marked deleterious effects on embryonic health. Bovine compact morulae were vitrified or slow-frozen, and post-warm morulae were cultured to the expanded blastocyst stage. Blastocysts developed from vitrified and slow-frozen morulae were subjected to microarray analysis and compared with blastocysts developed from unfrozen control morulae for differential gene expression. Morula to blastocyst conversion rate was higher (P < 0.05) in control (72%) and vitrified (77%) than in slow-frozen (34%) morulae...
2017: PloS One
https://www.readbyqxmd.com/read/29075189/a-short-term-exposure-to-tributyltin-blocks-leydig-cell-regeneration-in-the-adult-rat-testis
#20
Xiaolong Wu, Jianpeng Liu, Yue Duan, Shiyu Gao, Yao Lü, Xiaoheng Li, Qiqi Zhu, Xianwu Chen, Jing Lin, Leping Ye, Ren-Shan Ge
Background: Tributyltin (TBT) is widely used as an antifouling agent that may cause reproductive toxicity. The mechanism of TBT on Leydig cell development is still unknown. The objective of the present study was to investigate whether a brief exposure to low doses of TBT permanently affects Leydig cell development and to clarify the underlying mechanism. Methods: Adult male Sprague Dawley rats were randomly assigned into four groups and gavaged normal saline (control), 0.1, 1.0, or 10.0 mg/kg/day TBT for a consecutive 10 days, respectively...
2017: Frontiers in Pharmacology
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