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https://www.readbyqxmd.com/read/29726459/between-mofs-and-molecules-organolead-iv-compounds-with-chain-structures
#1
Abdel Fattah Shihada, Frank Weller, Werner Massa
Organolead compounds are of interest mainly as catalysts and organolead halides have proved to be very efficient materials for solar cells. Two organolead(IV) dimethylarsinates, namely catena-poly[[triphenyllead(IV)]-μ-chlorido-[triphenyllead(IV)]-μ-dimethylarsinato-κ2 O:O'], [Pb2 (C6 H5 )6 (C2 H6 AsO2 )Cl]n or [(Ph3 Pb)2 Cl(O2 AsMe2 )], (1), and poly[chlorido(μ3 -dimethylarsinato-κ3 O:O,O':O')diphenyllead(IV)], [Pb(C6 H5 )2 (C2 H6 AsO2 )Cl]n or [(Ph2 ClPb)(O2 AsMe2 )], (2), together with the triphenyllead(IV) diphenylphosphinate catena-poly[[triphenyllead(IV)]-μ-diphenylphosphinato-κ2 O:O'], [Pb(C6 H5 )3 (C12 H10 O2 P)]n or [(Ph3 Pb)(O2 PPh2 )], (3), have been synthesized and characterized by single-crystal X-ray diffraction, IR spectroscopy and mass spectrometry...
May 1, 2018: Acta Crystallographica. Section C, Structural Chemistry
https://www.readbyqxmd.com/read/29670056/isolation-and-identification-of-putative-protein-substrates-of-the-aaa-molecular-chaperone-clpb-from-the-pathogenic-spirochaete-leptospira-interrogans
#2
Joanna Krajewska, Zbigniew Arent, Michal Zolkiewski, Sabina Kędzierska-Mieszkowska
Bacterial ClpB is an ATP-dependent Hsp100 chaperone that reactivates aggregated proteins in cooperation with the DnaK chaperone system and promotes survival of bacteria under stress conditions. A large number of publications also indicate that ClpB supports the virulence of bacteria, including a pathogenic spirochaete Leptospira interrogans responsible for leptospirosis in both animals and humans. However, the exact role of ClpB in bacterial pathogenicity remains poorly characterized. It can be assumed that ClpB, due to its role as the molecular chaperone, mediates refolding of essential bacterial proteins, including the known virulence factors, which may become prone to aggregation under infection-induced stresses...
April 18, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29643454/activation-of-the-dnak-clpb-complex-is-regulated-by-the-properties-of-the-bound-substrate
#3
Jose Angel Fernández-Higuero, Alejandra Aguado, Judit Perales-Calvo, Fernando Moro, Arturo Muga
The chaperone ClpB in bacteria is responsible for the reactivation of aggregated proteins in collaboration with the DnaK system. Association of these chaperones at the aggregate surface stimulates ATP hydrolysis, which mediates substrate remodeling. However, a question that remains unanswered is whether the bichaperone complex can be selectively activated by substrates that require remodeling. We find that large aggregates or bulky, native-like substrates activates the complex, whereas a smaller, permanently unfolded protein or extended, short peptides fail to stimulate it...
April 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29554143/stress-related-genes-promote-edwardsiella-ictaluri-pathogenesis
#4
Ali Akgul, Ayfer Akgul, Mark L Lawrence, Attila Karsi
Edwardsiella ictaluri is a Gram-negative facultative anaerobic rod and the causative agent of enteric septicemia of channel catfish (ESC), which is one of the most prevalent diseases of catfish, causing significant economic losses in the catfish industry. E. ictaluri is resistant to complement system and macrophage killing, which results in rapid systemic septicemia. However, mechanisms of E. ictaluri stress responses under conditions of host environment are not studied well. Therefore, in this work, we report E...
2018: PloS One
https://www.readbyqxmd.com/read/29540723/comparative-roles-of-clpa-and-clpb-in-the-survival-of-s-typhimurium-under-stress-and-virulence-in-poultry
#5
Lal Sangpuii, Sunil Kumar Dixit, Manoj Kumawat, Shekhar Apoorva, Mukesh Kumar, Deepthi Kappala, Tapas Kumar Goswami, Manish Mahawar
By assisting in the proteolysis, disaggregation and refolding of the aggregated proteins, Caseinolytic proteases (Clps) enhance the cellular survival under stress conditions. In the current study, comparative roles of two such Clps, ClpA (involved in proteolysis) and ClpB (involved in protein disaggregation and refolding) in the survival of Salmonella Typhimurium (S. Typhimurium) under different stresses and in virulence have been investigated. clpA and clpB gene deletion mutant strains (∆clpA and ∆clpB) of S...
March 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29488359/secretome-profiling-of-propionibacterium-freudenreichii-reveals-highly-variable-responses-even-among-the-closely-related-strains
#6
Esther Frohnmeyer, Paulina Deptula, Tuula A Nyman, Pia K S Laine, Helena Vihinen, Lars Paulin, Petri Auvinen, Eija Jokitalo, Vieno Piironen, Pekka Varmanen, Kirsi Savijoki
This study compared the secretomes (proteins exported out of the cell) of Propionibacterium freudenreichii of different origin to identify plausible adaptation factors. Phylosecretomics indicated strain-specific variation in secretion of adhesins/invasins (SlpA, InlA), cell-wall hydrolysing (NlpC60 peptidase, transglycosylase), protective (RpfB) and moonlighting (DnaK, GroEL, GaPDH, IDH, ENO, ClpB) enzymes and/or proteins. Detailed secretome comparison suggested that one of the cereal strains (JS14) released a tip fimbrillin (FimB) in to the extracellular milieu, which was in line with the electron microscopy and genomic analyses, indicating the lack of surface-associated fimbrial-like structures, predicting a mutated type-2 fimbrial gene cluster (fimB-fimA-srtC2) and production of anchorless FimB...
May 2018: Microbial Biotechnology
https://www.readbyqxmd.com/read/29458868/stress-response-of-a-clinical-enterococcus-faecalis-isolate-subjected-to-a-novel-antimicrobial-surface-coating
#7
Emanuel Clauss-Lendzian, Ankita Vaishampayan, Anne de Jong, Uwe Landau, Carsten Meyer, Jan Kok, Elisabeth Grohmann
Emerging antibiotic resistance among pathogenic bacteria, paired with their ability to form biofilms on medical and technical devices, represents a serious problem for effective and long-term decontamination in health-care environments and gives rise to an urgent need for new antimicrobial materials. Here we present the impact of AGXX® , a novel broad-spectrum antimicrobial surface coating consisting of micro-galvanic elements formed by silver and ruthenium, on the transcriptome of Enterococcus faecalis. A clinical E...
March 2018: Microbiological Research
https://www.readbyqxmd.com/read/29246537/heat-shock-stress-profile-of-differential-expression-in-corynebacterium-pseudotuberculosis-biovar-equi
#8
Anne Cybelle Pinto Gomide, Pablo Gomes de Sá, Ana Lidia Queiroz Cavalcante, Thiago de Jesus Sousa, Lucas Gabriel Rodrigues Gomes, Rommel Thiago Juca Ramos, Vasco Azevedo, Artur Silva, Adriana Ribeiro Carneiro Folador
Transcriptome studies on Corynebacterium pseudotuberculosis have recently contributed to the understanding about this microorganism's survival mechanisms in various hostile conditions. The gene expression profile of the C. pseudotuberculosis strain 1002 (Ovis biovar), has revealed genes that are possible candidates responsible for its maintenance in adverse environments, such as those found in the host. In another strain of this bacterium, 258 (Equi biovar), a high temperature condition was simulated, in order to verify which genes are responsible for promoting the persistence of the bacterium in these conditions, since it tolerates temperatures higher than 40°C, despite being a mesophilic bacterium...
March 1, 2018: Gene
https://www.readbyqxmd.com/read/29156781/-toxoplasma-gondii-clp-family-protein-tg-clpb1-plays-a-crucial-role-in-thermotolerance
#9
Shinuo Cao, Nali Du, Heming Chen, Yu Pang, Zhaoxia Zhang, Jun Zheng, Honglin Jia
Caseinolytic peptidase B (ClpB) plays a pivotal role in suppressing and reversing protein aggregation. Toxoplasma gondii is an intracellular parasitic protozoan that infects a wide variety of mammals and birds and therefore is exposed to a broad range of living condition. We screened ToxoDB (http://ToxoDB.org) and identified 10 putative T. gondii genes encoding members of the Clp superfamily of caseinolytic proteases and chaperones. Of these, we focused on characterizing the Class I ATP-dependent molecular chaperones Tg ClpB1, Tg ClpB2, and Tg ClpB3...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29153776/francisella-noatunensis-subspecies-noatunensis-clpb-deletion-mutant-impairs-development-of-francisellosis-in-a-zebrafish-model
#10
Elisabeth O Lampe, Carl Zingmark, Julia I Tandberg, Ida Marie P Thrane, Espen Brudal, Anders Sjöstedt, Hanne C Winther-Larsen
BACKGROUND: Francisella noatunensis ssp. noatunensis (F.n.n.) is the causative agent of francisellosis in Atlantic cod and constitutes one of the main challenges for future aquaculture on this species. A facultative intracellular bacterium like F.n.n. exert an immunologic challenge against which live attenuated vaccines in general are most effective. Thus, we constructed a deletion in the F.n.n. clpB gene as ΔclpB mutants are among the most promising vaccine candidates in human pathogenic Francisella...
December 19, 2017: Vaccine
https://www.readbyqxmd.com/read/29094278/substrates-of-the-chloroplast-small-heat-shock-proteins-22e-f-point-to-thermolability-as-a-regulative-switch-for-heat-acclimation-in-chlamydomonas-reinhardtii
#11
Mark Rütgers, Ligia Segatto Muranaka, Timo Mühlhaus, Frederik Sommer, Sylvia Thoms, Juliane Schurig, Felix Willmund, Miriam Schulz-Raffelt, Michael Schroda
We have identified 39 proteins that interact directly or indirectly with high confidence with chloroplast HSP22E/F under heat stress thus revealing chloroplast processes affected by heat. Under conditions promoting protein unfolding, small heat shock proteins (sHsps) prevent the irreversible aggregation of unfolding proteins by integrating into forming aggregates. Aggregates containing sHsps facilitate the access of Hsp70 and ClpB/Hsp104 chaperones, which in ATP-dependent reactions disentangle individual proteins from the aggregates and assist in their refolding to the native state...
December 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/29032658/analysis-of-protein-expression-in-brucella-abortus-mutants-with-different-growth-rates-by-two-dimensional-gel-electrophoresis-and-lc-ms-ms-peptide-analysis
#12
Woo Bin Park, Young Bin Im, Shim Soojin, Han Sang Yoo
Brucella abortus is a bacterium that causes brucellosis and is the causative agent of worldwide zoonosis. Pathogenesis of the Brucella abortus infection is so complicates. Thus, several researches have made to solve mechanism in infection of Brucella abortus. While several proteins are revealed as the pathogenic factors by previous researches, underlying mechanism of the Brucella abortus infection is still left to be resolved. We identify the proteins showing the different expression level in Brucella abortus mutants with different biological characteristics that generated by random insertion of transposon...
October 13, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28982315/bacterial-protein-mimetic-of-peptide-hormone-as-a-new-class-of-protein-based-drugs
#13
S O Fetissov, Romain Legrand, Nicolas Lucas
Background Specific peptide molecules classified as hormones, neuropeptides and cytokines are involved in intercellular signaling regulating various physiological processes in all organs and tissues. This justifies the peptidergic signaling as an attractive pharmacological target. Recently, a protein mimetic of a peptide hormone has been identified in Escherichia coli suggesting the potential use of specific bacterial proteins as a new type of peptide-like drugs. Objective This paper describes the scientific rational and the technological approach leading to the identification of the E...
October 5, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28979246/variable-persister-gene-interactions-with-p-ppgpp-for-persister-formation-in-escherichia-coli
#14
Shuang Liu, Nan Wu, Shanshan Zhang, Youhua Yuan, Wenhong Zhang, Ying Zhang
Persisters comprise a group of phenotypically heterogeneous metabolically quiescent bacteria with multidrug tolerance and contribute to the recalcitrance of chronic infections. Although recent work has shown that toxin-antitoxin (TA) system HipAB depends on stringent response effector (p)ppGppin persister formation, whether other persister pathways are also dependent on stringent response has not been explored. Here we examined the relationship of (p)ppGpp with 15 common persister genes (dnaK, clpB, rpoS, pspF, tnaA, sucB, ssrA, smpB, recA, umuD, uvrA, hipA, mqsR, relE, dinJ) using Escherichia coli as a model...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28930669/a-highly-specific-escherichia-coli-qpcr-and-its-comparison-with-existing-methods-for-environmental-waters
#15
David I Walker, Jonathan McQuillan, Michael Taiwo, Rachel Parks, Craig A Stenton, Hywel Morgan, Matthew C Mowlem, David N Lees
The presence of Escherichia coli in environmental waters is considered as evidence of faecal contamination and is therefore commonly used as an indicator in both water quality and food safety analysis. The long period of time between sample collection and obtaining results from existing culture based methods means that contamination events may already impact public health by the time they are detected. The adoption of molecular based methods for E. coli could significantly reduce the time to detection. A new quantitative real-time PCR (qPCR) assay was developed to detect the ybbW gene sequence, which was found to be 100% exclusive and inclusive (specific and sensitive) for E...
December 1, 2017: Water Research
https://www.readbyqxmd.com/read/28879184/hsp78-78-kda-heat-shock-protein-a-representative-aaa-family-member-found-in-the-mitochondrial-matrix-of-saccharomyces-cerevisiae
#16
REVIEW
Josielle Abrahão, David Z Mokry, Carlos H I Ramos
ATPases associated with diverse cellular activities (AAA+) form a superfamily of proteins involved in a variety of functions and are characterized by the presence of an ATPase module containing two conserved motifs known as Walker A and Walker B. ClpB and Hsp104, chaperones that have disaggregase activities, are members of a subset of this superfamily, known as the AAA family, and are characterized by the presence of a second highly conserved motif, known as the second region of homology (SRH). Hsp104 and its homolog Hsp78 (78 kDa heat shock protein) are representatives of the Clp family in yeast...
2017: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/28873066/preliminary-study-of-the-association-between-the-elimination-parameters-of-phenytoin-and-phenobarbital
#17
Janthima Methaneethorn, Duangchit Panomvana, Thaveechai Vachirayonstien
BACKGROUND: Therapeutic drug monitoring is essential for both phenytoin and phenobarbital therapy given their narrow therapeutic indexes. Nevertheless, the measurement of either phenytoin or phenobarbital concentrations might not be available in some rural hospitals. Information assisting individualized phenytoin and phenobarbital combination therapy is important. This study's objective was to determine the relationship between the maximum rate of metabolism of phenytoin (Vmax) and phenobarbital clearance (CLPB), which can serve as a guide to individualized drug therapy...
September 26, 2017: Drug Metabolism and Personalized Therapy
https://www.readbyqxmd.com/read/28819163/fusion-protein-analysis-reveals-the-precise-regulation-between-hsp70-and-hsp100-during-protein-disaggregation
#18
Sayaka Hayashi, Yosuke Nakazaki, Kei Kagii, Hiromi Imamura, Yo-Hei Watanabe
ClpB, a bacterial Hsp100, is a ring-shaped AAA+ chaperone that can reactivate aggregated proteins in cooperation with DnaK, a bacterial Hsp70, and its co-factors. ClpB subunits comprise two AAA+ modules with an interstitial rod-shaped M-domain. The M-domain regulates ClpB ATPase activity and interacts directly with the DnaK nucleotide-binding domain (NBD). Here, to clarify how these functions contribute to the disaggregation process, we constructed ClpB, DnaK, and aggregated YFP fusion proteins in various combinations...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28798962/structural-pathway-of-regulated-substrate-transfer-and-threading-through-an-hsp100-disaggregase
#19
Célia Deville, Marta Carroni, Kamila B Franke, Maya Topf, Bernd Bukau, Axel Mogk, Helen R Saibil
Refolding aggregated proteins is essential in combating cellular proteotoxic stress. Together with Hsp70, Hsp100 chaperones, including Escherichia coli ClpB, form a powerful disaggregation machine that threads aggregated polypeptides through the central pore of tandem adenosine triphosphatase (ATPase) rings. To visualize protein disaggregation, we determined cryo-electron microscopy structures of inactive and substrate-bound ClpB in the presence of adenosine 5'- O -(3-thiotriphosphate), revealing closed AAA+ rings with a pronounced seam...
August 2017: Science Advances
https://www.readbyqxmd.com/read/28767525/comparison-of-lower-limb-and-back-exercises-for-runners-with-chronic-low-back-pain
#20
RANDOMIZED CONTROLLED TRIAL
Congcong Cai, Yifan Yang, Pui W Kong
INTRODUCTION: This single-blind randomized trial was conducted to compare the treatment effect of lower limb (LL) exercises versus conventional lumbar extensor (LE) and lumbar stabilization (LS) exercises in recreational runners with chronic low back pain (cLBP), because there is currently no specific protocol for managing runners with cLBP. METHODS: Eighty-four recreational runners with cLBP were allocated to three exercise groups (LL, LE, LS) for an 8-wk intervention...
December 2017: Medicine and Science in Sports and Exercise
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