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Mitochondrial permeability transition pore

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https://www.readbyqxmd.com/read/29136370/ochratoxin-a-induced-apoptosis-of-ipec-j2-cells-through-ros-mediated-mitochondrial-permeability-transition-pore-opening-pathway
#1
Hong Wang, Ying Chen, Nianhui Zhai, Xingxiang Chen, Fang Gan, Hu Li, Kehe Huang
With the purpose to explore the mechanisms associated with the intestinal toxicity of OTA, an intestinal porcine epithelial cell line (IPEC-J2) was applied in this study as in vitro models for intestinal epithelium. The results confirmed that OTA induced IPEC-J2 cell toxicity by MTT assay and apoptosis by Hoechst 33258 staining and flow cytometer analysis. And then, we observed that OTA induced the mitochondrial reactive oxygen species (ROS) production and mitochondrial permeability transition pore (mPTP) opening by confocal microscopy...
November 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29125839/csseverin-inhibits-apoptosis-through-mitochondria-mediated-pathways-triggered-by-ca2-dyshomeostasis-in-hepatocarcinoma-plc-cells
#2
Mengchen Shi, Lina Zhou, Lu Zhao, Mei Shang, Tongtong He, Zeli Tang, Hengchang Sun, Pengli Ren, Zhipeng Lin, Tingjin Chen, Jinyun Yu, Jin Xu, Xinbing Yu, Yan Huang
BACKGROUND: Numerous experimental and epidemiological studies have demonstrated a link between Clonorchis sinensis (C. sinensis) infestation and cholangiocarcinoma (CCA) as well as hepatocellular carcinoma (HCC). The underlying molecular mechanism involved in the malignancy of CCA and HCC has not yet been addressed. Csseverin, a component of the excretory/secretory products of C. sinensis (CsESPs), was confirmed to cause obvious apoptotic inhibition in the human HCC cell line PLC. However, the antiapoptotic mechanism is unclear...
November 10, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29120799/study-on-the-effect-of-reactive-oxygen-species-mediated-oxidative-stress-on-the-activation-of-mitochondrial-apoptosis-and-the-tenderness-of-yak-meat
#3
Lin-Lin Wang, Qun-Li Yu, Ling Han, Xiu-Li Ma, Ren-De Song, Suo-Nan Zhao, Wen-Hua Zhang
This study investigated the effect of reactive oxygen species-mediated oxidative stress on activation of mitochondrial apoptosis and tenderness of yak meat during postmortem ageing. Oxidative stress degree, Ca(2+) levels, membrane permeability transition pore opening, mitochondrial membrane potential, apoptotic factors and the shear force were examined. Results showed that the ROS generated by H2O2 significantly increased mitochondrial oxidative stress by decreasing the activities of superoxide dismutase, catalase and glutathione peroxidase, and increasing lipid peroxidation...
April 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29117569/ros-control-mitochondrial-motility-through-p38-and-the-motor-adaptor-miro-trak
#4
Valentina Debattisti, Akos A Gerencser, Masao Saotome, Sudipto Das, György Hajnóczky
Mitochondrial distribution and motility are recognized as central to many cellular functions, but their regulation by signaling mechanisms remains to be elucidated. Here, we report that reactive oxygen species (ROS), either derived from an extracellular source or intracellularly generated, control mitochondrial distribution and function by dose-dependently, specifically, and reversibly decreasing mitochondrial motility in both rat hippocampal primary cultured neurons and cell lines. ROS decrease motility independently of cytoplasmic [Ca(2+)], mitochondrial membrane potential, or permeability transition pore opening, known effectors of oxidative stress...
November 7, 2017: Cell Reports
https://www.readbyqxmd.com/read/29115483/hepatocellular-toxicity-of-oxalicumone-a-via-oxidative-stress-injury-and-mitochondrial-dysfunction-in-healthy-human-liver-cells
#5
Si Shi, Limei Yao, Kunbin Guo, Xiangyu Wang, Qi Wang, Weirong Li
The marine‑derived oxalicumone A (POA) has been demonstrated as a potent anti‑tumor bioactive agent for a variety of human carcinoma, but to the best of our knowledge, remains to be evaluated in healthy liver cells. As many drugs distribute preferentially in the liver, the present study aimed to investigate the effects of POA on apoptosis, oxidative stress and mitochondrial function in L‑02 healthy liver cells. A Cell‑Counting kit‑8 assay demonstrated that POA inhibits the proliferation of L‑02 cells in a dose‑ and time‑dependent manner...
November 6, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29107253/attribution-of-bax-and-mitochondrial-permeability-transition-pore-on-cantharidin-induced-apoptosis-of-sf9-cells
#6
Gaofeng Cui, Yuansheng Li, Kai Ding, Shaodong Hao, Jinzhong Wang, Zhiyong Zhang
To investigate the insecticidal mechanism of cantharidin, a promising biological pesticide substance from blister beetle, on Sf9 cells, a cultured cell line derived from fall armyworm, Spodoptera frugiperda, we preliminary studied the attribution of Bax channel and mitochondrial permeability transition pore on cantharidin-induced mitochondrial apoptosis signal pathway. Changes in cell morphology, activity of mitochondrial dehydrogenases, release of cytochrome C and mitochondrial transmembrane potential were detected when the two channels were blocked by specific inhibitors, Bax channel blocker and cyclosporin A...
October 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/29101324/cyclophilin-d-regulates-the-dynamic-assembly-of-mitochondrial-atp-synthase-into-synthasomes
#7
Gisela Beutner, Ryan E Alanzalon, George A Porter
Mitochondrial electron transport is essential for oxidative phosphorylation (OXPHOS). Electron transport chain (ETC) activity generates an electrochemical gradient that is used by the ATP synthase to make ATP. ATP synthase is organized into supramolecular units called synthasomes that increase the efficiency of ATP production, while within ATP synthase is the cyclophilin D (CypD) regulated mitochondrial permeability transition pore (PTP). We investigated whether synthasomes are dynamic structures that respond to metabolic demands and whether CypD regulates this dynamic...
November 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29089467/suppression-of-stim1-reduced-intracellular-calcium-concentration-and-attenuated-hypoxia-reoxygenation-induced-apoptosis-in-h9c2-cells
#8
Fei He, Qianfu Wu, Banglong Xu, Xiaocheng Wang, Jixiong Wu, Li Huang, Jing Cheng
Objective: Previous studies have demonstrated STIM1-mediated store-operated Ca(2+) entry(SOCE) contributes to intracellular Ca(2+) accumulation. The present study aimed to investigate the expression of STIM1 and Orai1/TRPC1 in the context of myocardial ischemia/reperfusion injury(MIRI) and the effect of STIM1 inhibition on Ca(2+) accumulation and apoptosis in H9c2 cardiomyocytes subjected to hypoxia/reoxygenation(H/R). Methods: Expression of STIM1/Orai1/TRPC1 was determined by RT-PCR and Western Blot in mice subjected to MIRI and H9C2 cardiomyocytes subjected to H/R...
October 31, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29045576/activation-of-cannabinoid-receptor-1-is-involved-in-protection-against-mitochondrial-dysfunction-and-cerebral-ischaemic-tolerance-induced-by-isoflurane-preconditioning
#9
M Cai, Q Yang, G Li, S Sun, Y Chen, L Tian, H Dong
Background: Isoflurane preconditioning (IPC) induces cerebral ischaemic tolerance, but the mechanism remains poorly understood. The aim of this study was to determine changes in mitochondrial function in the brain after IPC, and whether the cannabinoid receptor 1 (CB1R) could be involved in the mechanism of mitochondrial protection mediated by IPC. Methods: Adult male Sprague-Dawley rats were pretreated with isoflurane 2% for 1 h day-1, for 5 days consecutively, and then subjected to 120 min right middle cerebral artery occlusion...
October 13, 2017: British Journal of Anaesthesia
https://www.readbyqxmd.com/read/29043508/the-role-of-pi3k%C3%AE-isoform-in-cardioprotection
#10
Xavier Rossello, Jaime A Riquelme, Zhenhe He, Stasa Taferner, Bart Vanhaesebroeck, Sean M Davidson, Derek M Yellon
Ischemic preconditioning (IPC) limits myocardial infarct size through the activation of the PI3K-Akt signal cascade; however, little is known about the roles of individual PI3K isoforms in cardioprotection. We aimed, therefore, to elucidate the role of the PI3Kα isoform in cardioprotection Pharmacological PI3Kα inhibition was assessed in isolated-perfused mouse hearts subjected to ischemia/reperfusion injury (IRI), either during the IPC procedure or at reperfusion. PI3Kα inhibition abrogated the IPC-induced protective effect at reperfusion, but not when given only during the IPC protocol...
October 17, 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/29040818/metformin-attenuates-er-stress-induced-mitochondrial-dysfunction
#11
Qun Chen, Jeremy Thompson, Ying Hu, Anindita Das, Edward J Lesnefsky
Endoplasmic reticulum (ER) stress, a disturbance of the ER function, contributes to cardiac injury. ER and mitochondria are closely connected organelles within cells. ER stress contributes to mitochondrial dysfunction, which is a key factor to increase cardiac injury. Metformin, a traditional anti-diabetic drug, decreases cardiac injury during ischemia-reperfusion. Metformin also inhibits ER stress in cultured cells. We hypothesized that metformin can attenuate the ER stress-induced mitochondrial dysfunction and subsequent cardiac injury...
September 28, 2017: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/29031076/synthesis-and-evaluation-of-new-pyridyl-pyrazinyl-thiourea-derivatives-neuroprotection-against-amyloid-%C3%AE-induced-toxicity
#12
Jung-Eun Park, Ahmed Elkamhawy, Ahmed H E Hassan, Ae Nim Pae, Jiyoun Lee, Sora Paik, Beoung-Geon Park, Eun Joo Roh
Herein, we report synthesis and evaluation of new twenty six small molecules against β amyloid (Aβ)-induced opening of mitochondrial permeability transition pore (mPTP) using JC-1 assay which measures the change of mitochondrial membrane potential (ΔΨm). The neuroprotective effect of seventeen compounds against Aβ-induced mPTP opening was superior to that of the standard Cyclosporin A (CsA). Fifteen derivatives eliciting increased green to red fluorescence percentage less than 40.0% were evaluated for their impact on ATP production, cell viability and neuroprotection against Aβ-induced neuronal cell death...
December 1, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28990067/thevetiaflavone-from-wikstroemia%C3%A2-indica-ameliorates-pc12-cells-injury-induced-by-ogd-r-via-improving-ros%C3%A2-mediated-mitochondrial-dysfunction
#13
Huankai Yao, Zhenhai Yuan, Guangming Wei, Chun Chen, Jingyu Duan, Yan Li, Yunru Wang, Chunping Zhang, Yuanhua Liu
Cerebral ischemia and following reperfusion affects many people worldwide. To discover efficient therapeutic approaches, numerous natural products have been investigated. The current study investigated the protective effects of thevetiaflavone, a natural flavonoid obtained from Wikstroemia indica, and the associated mechanisms using PC12 cells induced by oxygen and glucose deprivation. As a result, thevetiaflavone improves cell viability and suppresses the leakage of lactate dehydrogenase from the cytoplasm...
December 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28986220/two-mutations-in-mitochondrial-atp6-gene-of-atp-synthase-related-to-human-cancer-affect-ros-calcium-homeostasis-and-mitochondrial-permeability-transition-in-yeast
#14
Katarzyna Niedzwiecka, Renata Tisi, Sara Penna, Malgorzata Lichocka, Danuta Plochocka, Roza Kucharczyk
The relevance of mitochondrial DNA (mtDNA) mutations in cancer process is still unknown. Since the mutagenesis of mitochondrial genome in mammals is not possible yet, we have exploited budding yeast S. cerevisiae as a model to study the effects of tumor-associated mutations in the mitochondrial MTATP6 gene, encoding subunit 6 of ATP synthase, on the energy metabolism. We previously reported that four mutations in this gene have a limited impact on the production of cellular energy. Here we show that two mutations, Atp6-P163S and Atp6-K90E (human MTATP6-P136S and MTATP6-K64E, found in prostate and thyroid cancer samples, respectively), increase sensitivity of yeast cells both to compounds inducing oxidative stress and to high concentrations of calcium ions in the medium, when Om45p, the component of porin complex in outer mitochondrial membrane (OM), was fused to GFP...
October 3, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28967329/post-injury-treatment-with-nim811-promotes-recovery-of-function-in-adult-female-rats-following-spinal-cord-contusion-a-dose-response-study
#15
Joe E Springer, Nishant Visavadiya, Patrick G Sullivan, Edward D Hall
Mitochondrial homeostasis is essential for maintaining cellular function and survival in the central nervous system (CNS). Mitochondrial function is significantly compromised following spinal cord injury (SCI) and is associated with accumulation of high levels of calcium, increased production of free radicals, oxidative damage, and eventually mitochondrial permeability transition (mPT). The formation of the mPT pore (mPTP) and subsequent mPT state are considered to be end stage events in the decline of mitochondrial integrity, and strategies that inhibit mPT can limit mitochondrial demise...
October 1, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/28954424/mitochondriotropic-and-cardioprotective-effects-of-triphenylphosphonium-conjugated-derivatives-of-the-diterpenoid-isosteviol
#16
Lara Testai, Irina Strobykina, Victor V Semenov, Marina Semenova, Eleonora Da Pozzo, Alma Martelli, Valentina Citi, Claudia Martini, Maria C Breschi, Vladimir E Kataev, Vincenzo Calderone
Mitochondria play a crucial role in the cell fate; in particular, reducing the accumulation of calcium in the mitochondrial matrix offers cardioprotection. This affect is achieved by a mild depolarization of the mitochondrial membrane potential, which prevents the assembly and opening of the mitochondrial permeability transition pore. For this reason, mitochondria are an attractive target for pharmacological interventions that prevent ischaemia/reperfusion injury. Isosteviol is a diterpenoid created from the acid hydrolysis of Steviarebaudiana Bertoni (fam...
September 26, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28942440/epigallocatechin-3-gallate-ameliorates-angiotensin-ii-induced-oxidative-stress-and-apoptosis-in-human-umbilical-vein-endothelial-cells-through-the-activation-of-nrf2-caspase-3-signaling
#17
Xiuli Zhou, Liwen Liang, Yan Zhao, Hong Zhang
INTRODUCTION: This study aimed to investigate whether epigallocatechin-3-gallate (EGCG) shows antioxidant activity against angiotensin II (Ang II)-induced human umbilical vein endothelial cell (HUVEC) apoptosis. MATERIALS AND METHODS: The viability of HUVECs was revealed by MTT and LDH assay. The cell apoptosis was detected by FITC-PI assay. A fluorescent probe assay was used to measure the reactive oxygen species (ROS) generation in HUVECs. Mitochondrial permeability transition pore (MPTP) opening, mitochondrial membrane potential, and caspase-3, -4, -8, -9 activities were also measured...
2017: Journal of Vascular Research
https://www.readbyqxmd.com/read/28922345/tilianin-post-conditioning-attenuates-myocardial-ischemia-reperfusion-injury-via-mitochondrial-protection-and-inhibition-of-apoptosis
#18
Yangyang Wang, Yong Yuan, Xinchun Wang, Yanfang Wang, Jiang Cheng, Li Tian, Xinhong Guo, Dongmei Qin, Wenjiang Cao
BACKGROUND The aim of this study was to investigate the role of tilianin in modulating mitochondrial functions and mitochondria-mediated apoptosis during cardio-protection. MATERIAL AND METHODS Myocardial ischemia/reperfusion (I/R) injury was induced by 30 minutes coronary occlusion followed by two hours reperfusion in Sprague-Dawley rats. To investigate the cardio-protective effects of tilianin, apoptosis was evaluated by TUNEL. Mitochondrial ultrastructure and function were assessed by transmission electron microscopy, dynamics of mitochondrial permeability transition pore (mPTP) opening and ATP production in the myocardium; Ca2+ content and reactive oxygen species (ROS) were measured to evaluated the level of damage factors in the mitochondria...
September 18, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28921048/antibacterial-and-antitumor-activity-of-bogorol-b-jx-isolated-from-brevibacillus-laterosporus-jx-5
#19
Hongxia Jiang, Chao Ji, Junkang Sui, Rongbo Sa, Xiaohui Wang, Xunli Liu, Tai L Guo
Antimicrobial peptides are promising anti-infective agent candidates because they have a broad antimicrobial spectrum and bioactivity and are unlikely to elicit antibiotic resistance. The bogorols represent a new cationic antibiotic peptide and possess great therapeutic potential because of their bioactivity and precise mode of action. Here, we report that Bogorol B-JX (BBJX), a peptide previously isolated from Brevibacillus laterosporus JX-5 by us, has significant antibacterial and antitumor activities in vitro...
September 18, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28918598/triacsin-c-reduces-lipid-droplet-formation-and-induces-mitochondrial-biogenesis-in-primary-rat-hepatocytes
#20
Carlos R P Dechandt, Felippe H Zuccolotto-Dos-Reis, Bruno G Teodoro, Anna Maria A P Fernandes, Marcos N Eberlin, Isis C Kettelhut, Carlos Curti, Luciane C Alberici
Intracellular long-chain acyl-CoA synthetases (ACSL) activate fatty acids to produce acyl-CoA, which undergoes β-oxidation and participates in the synthesis of esterified lipids such as triacylglycerol (TAG). Imbalances in these metabolic routes are closely associated with the pathogenesis of non-alcoholic fatty liver disease (NAFLD). Triacsin C is one of the few compounds that inhibit TAG accumulation into lipid droplets (LD) by suppressing ACSL activity. Here we report that treatment of primary rat hepatocytes with triacsin C at concentrations lower than the IC50 (4...
September 16, 2017: Journal of Bioenergetics and Biomembranes
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