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Amita Gupta, Michael A Hicks, Shawn P Manchester, Kristala L J Prather
D-Glucaric acid can be produced as a value-added chemical from biomass through a de novo pathway in Escherichia coli. However, previous studies have identified pH-mediated toxicity at product concentrations of 5 g/L and have also found the eukaryotic myo-inositol oxygenase (MIOX) enzyme to be rate-limiting. We ported this pathway to Saccaromyces cerevisiae, which is naturally acid-tolerant and evaluate a codon-optimized MIOX homologue. We constructed two engineered yeast strains that were distinguished solely by their MIOX gene - either the previous version from Mus musculus or a homologue from Arabidopsis thaliana codon-optimized for expression in S...
September 2016: Biotechnology Journal
Mingxue Liu, Faqin Dong, Wei Zhang, Xiaoqin Nie, Shiyong Sun, Hongfu Wei, Lang Luo, Sha Xiang, Gege Zhang
One of the waste disposal principles is decrement. The programmed gradient descent biosorption of strontium ions by Saccaromyces cerevisiae regarding bioremoval and ashing process for decrement were studied in present research. The results indicated that S. cerevisiae cells showed valid biosorption for strontium ions with greater than 90% bioremoval efficiency for high concentration strontium ions under batch culture conditions. The S. cerevisiae cells bioaccumulated approximately 10% of strontium ions in the cytoplasm besides adsorbing 90% strontium ions on cell wall...
August 15, 2016: Journal of Hazardous Materials
Silvia Jane Lombardi, Antonella De Leonardis, Giuseppe Lustrato, Bruno Testa, Massimo Iorizzo
BACKGROUND: Sparkling wines produced by traditional method owe their characteristics to secondary fermentation and maturation that occur during a slow ageing in bottles. Yeast autolysis plays an important role during the sparkling wine aging. Using a combination of killer and sensitive yeasts is possible to accelerate yeast autolysis and reduce maturing time. METHODS: killer and sensitive Saccharomyces cerevisiae strains, separately and in co-cultures, were inoculated in base wine and bottled on pilot-plant scale...
2015: Recent Patents on Biotechnology
Ethiene Castellucci Estevam, Sharoon Griffin, Muhammad Jawad Nasim, Polina Denezhkin, Ramona Schneider, Rainer Lilischkis, Enrique Dominguez-Alvarez, Karolina Witek, Gniewomir Latacz, Cornelia Keck, Karl-Herbert Schäfer, Katarzyna Kieć-Kononowicz, Jadwiga Handzlik, Claus Jacob
Various bacteria, including diverse Staphylococci, reduce selenite to yield red selenium particles with diameters in the high nanometer to low micrometer range. Formation and accumulation of such particles in bacteria often results in cell death, triggered by a loss of thiols and formation of disruptive deposits inside the cell. Hence certain pathogenic bacteria are rather sensitive to the presence of selenite, whilst other organisms, such as small nematodes, do not employ this kind of nanotechnology, yet become affected by micromolar concentrations of such naturally generated materials...
February 15, 2017: Journal of Hazardous Materials
Toshihiko Ohnuki, Fuminori Sakamoto, Shinya Yamasaki, Naofumi Kozai, Hiroyuki Shiotsu, Satoshi Utsunomiya, Naoko Watanabe, Tamotsu Kozaki
The accumulation of Cs by unicellular fungus of Saccharomyces cerevisiae in the presence of minerals has been studied to elucidate the role of microorganisms in the migration of radioactive Cs in the environment. Two different types of experiments were employed: experiments using stable Cs to examine the effect of a carbon source on the accumulation of Cs, and accumulation experiments of radioactive Cs from agar medium containing (137)Cs and zeolite, vermiculite, phlogopite, smectite, mica, or illite as mineral supplements...
June 2015: Journal of Environmental Radioactivity
Homayoun Zojaji, Mehdi Ghobakhlou, Hassan Rajabalinia, Elnaz Ataei, Somayeh Jahani Sherafat, Bijan Moghimi-Dehkordi, Rasoul Bahreiny
AIM: Evaluating the efficacy and safety of adding the probiotic Saccharomyces boulardiito standard triple therapy for eradication of Helicobacter pylori. BACKGROUND: Several probiotics such as Saccharomyces boulardii have been investigated for their clinical efficacy. This probiotic, inhibit H. pylori urease by lowering the gastric pH, adhesion of H. pylori to gastric epithelial cells, stabilize the gastric barrier function and reduce the side effects of antibiotics...
2013: Gastroenterology and Hepatology From Bed to Bench
Mauro Bafutto, José Roberto de Almeida, Nayle Vilela Leite, Michelle Bafutto Gomes Costa, Enio Chaves de Oliveira, Joffre Resende-Filho
CONTEXT: Irritable bowel syndrome (IBS) is a functional bowel disease characterized by abdominal pain and altered intestinal habits. The pathophysiology of IBS remains unclear. Recent studies have demonstrated that some IBS patients, especially in diarrhea-predominant IBS (IBS-D), display persistent signs of minor mucosal inflammation and a modified intestinal microflora. The mesalazine has known intestinal anti-inflammatory properties. Saccharomyces boulardii is a probiotic used for a long time in treatment of diarrhea, including infectious diarrhea...
October 2013: Arquivos de Gastroenterologia
G Gessoni, F Antico, D Caroli, A Nogara, S Valverde, M Fezzi, M Zucchelli, A Boscolo-Bariga
In type 3 polyendocrine syndrome (PAS3), autoimmune thyroiditis occurs with other organ-specific autoimmune disease, but not with autoimmune adrenalitis. In this report we described a family from Pakistan in which mother and three daughters were affected by a PAS3. We studied a family from Pakistan: Father MMu age 44, mother KN aged 44, three daughters MM age 20, MH age 16 and MA age 14 and a son MU age 18. These subjects were tested for thyroids function, metabolic function, adrenal function, autoimmune disease...
September 2013: Minerva Endocrinologica
Brindusa Truta, Dan X Li, Uma Mahadevan, Elena R Fisher, Yunn-Y Chen, Kim Grace, Fernando Velayos, Jonathan P Terdiman
BACKGROUND AND AIMS: One of the causes of pouch failure after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) is the development of de novo Crohn's disease (CD). Our aim was to clearly define factors associated with post-IPAA CD. METHODS: We conducted a cross-sectional study to compare demographic, clinical, and serological characteristics of patients with and without post-IPAA CD. All subjects underwent testing for anti-neutrophil cytoplasm antibodies, anti-Saccaromyces cerevisiae antibodies, anti-outer membrane porin C antibodies, and anti-CBir1 flagellin (anti-CBir1)...
January 2014: Digestive Diseases and Sciences
György Miklós Buzás
After a short overview of the history of probiotics, the author presents the development of human intestinal microflora based on the newest genetic data and the microbiological features of main probiotics. The indications of probiotic administration have been defined and extended in recent years. The author reviews significant results of probiotic treatment in some gastrointestinal diseases based on meta-analytical data. Probiotics are useful in preventing and treating diarrhoea caused by antibiotics and Clostridium difficile caused diarrhoea...
February 24, 2013: Orvosi Hetilap
Ee Li, Feng Yue, Qi Chang, Xuena Guo, Xiuping He, Borun Zhang
Flocculation is an attractive property for Saccaromyces cerevisiae, which plays important roles in fermentation industry and environmental remediation. The process of flocculation is mediated by a family of cell surface flocculins. As one member of flocculins, Flo1 is characterized by four families of repeats (designated as repeat units A, B, C and D) in the central domain. It is generally accepted that variation of repeat unit A in length in Flo1 influences the degree of flocculation or specificity for sugar recognization...
2013: PloS One
Onn Brandman, Jacob Stewart-Ornstein, Daisy Wong, Adam Larson, Christopher C Williams, Gene-Wei Li, Sharleen Zhou, David King, Peter S Shen, Jimena Weibezahn, Joshua G Dunn, Silvi Rouskin, Toshifumi Inada, Adam Frost, Jonathan S Weissman
The conserved transcriptional regulator heat shock factor 1 (Hsf1) is a key sensor of proteotoxic and other stress in the eukaryotic cytosol. We surveyed Hsf1 activity in a genome-wide loss-of-function library in Saccaromyces cerevisiae as well as ~78,000 double mutants and found Hsf1 activity to be modulated by highly diverse stresses. These included disruption of a ribosome-bound complex we named the Ribosome Quality Control Complex (RQC) comprising the Ltn1 E3 ubiquitin ligase, two highly conserved but poorly characterized proteins (Tae2 and Rqc1), and Cdc48 and its cofactors...
November 21, 2012: Cell
Maria Staiano, Maria Strianese, Antonio Varriale, Stefano Di Giovanni, Donatella Scotto di Mase, Valentina Dell'Angelo, Giuseppe Ruggiero, Tullio Labella, Claudio Pellecchia, Sabato D'Auria
The Saccaromices cerevisiae D-serine dehydratase is a pyridoxal 5'-phosphate dependent enzyme that requires zinc for its function. It catalyses the conversion of D-serine into pyruvate and ammonia with the K(m) and k(cat) values of 0.39 mM and 13.1 s(-1) respectively. In this work, a new methodology for monitoring D-serine is presented. Our results show that this enzyme could be successfully used as a biological probe for detection of D-serine via fluorescence spectroscopy.
June 1, 2012: Protein and Peptide Letters
Maria Pia Santacroce, Elisabetta Merra, Gerardo Centoducati, Valentina Zacchino, Elisabetta Casalino
The main goal of this work was to determine the effect of dietary live yeast Saccharomyces cerevisiae on the oxidative status of sea bass Dicentrarchus labrax juveniles. Fishes were fed on three diets: the GM group were fed a diet containing lyophilized yeast grown on grape must, the CS group were fed a diet containing lyophilized yeast grown on cornstarch, and the control group were fed a diet without yeast. The activity of the main antioxidative enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase, glutathione S-transferase (GST), and glutathione (GSH) content, as well as lipid peroxidation, was measured in the liver of sea bass juveniles 90 days after hatching...
October 2012: Fish Physiology and Biochemistry
Yuan Wu, Peng-Cheng Du, Wen-Ge Li, Jin-Xing Lu
Feral pigeons are known as reservoirs of pathogenic yeasts that cause opportunistic infections in human. In the outskirts of Beijing, China, pigeons are more frequently raised at homes than are encountered in public areas. Many studies have focused on the presence of pathogenic yeasts in the excreta (fresh or withered) of a variety kinds of birds, pigeon crop and cloacae. One hundred and forty-three samples of fresh droppings were collected from three suburban pigeon-raising homes in an area of northern Beijing, China...
September 2012: Mycopathologia
Sven Vilain, Giovanni Esposito, Dominik Haddad, Onno Schaap, Mariya P Dobreva, Melissa Vos, Stefanie Van Meensel, Vanessa A Morais, Bart De Strooper, Patrik Verstreken
Pink1 is a mitochondrial kinase involved in Parkinson's disease, and loss of Pink1 function affects mitochondrial morphology via a pathway involving Parkin and components of the mitochondrial remodeling machinery. Pink1 loss also affects the enzymatic activity of isolated Complex I of the electron transport chain (ETC); however, the primary defect in pink1 mutants is unclear. We tested the hypothesis that ETC deficiency is upstream of other pink1-associated phenotypes. We expressed Saccaromyces cerevisiae Ndi1p, an enzyme that bypasses ETC Complex I, or sea squirt Ciona intestinalis AOX, an enzyme that bypasses ETC Complex III and IV, in pink1 mutant Drosophila and find that expression of Ndi1p, but not of AOX, rescues pink1-associated defects...
January 2012: PLoS Genetics
Federico José Vicente Gomez, Julio Raba, Soledad Cerutti, María Fernanda Silva
Several studies have shown the presence of melatonin and related compounds in grapes and wines. The latter provides evidence of the possibility to enhance the nutraceutical properties of premium wines. However, there are many external factors that can influence the levels of this indolamine in grape and wines. In this study, the monitoring of melatonin and its tentatively identified isomer was carried out during the entire winemaking process in Vitis vinifera cv. Malbec by ultra high-performance liquid chromatography-tandem mass spectrometry...
April 2012: Journal of Pineal Research
Kirang Kim, Yoon Jung Yang, Kyunga Kim, Mi Kyung Kim
BACKGROUND: Gene-nutrient interactions may be important in modulating susceptibility to metabolic disorders. OBJECTIVES: The objectives of this study were to assess the association of dietary calcium intake with the risk of metabolic syndrome and to investigate the interaction effects between dietary calcium intake and candidate gene polymorphisms. DESIGN: Subjects were participants in the Korea Association Resource project, which was initiated in 2007 as a large-scale, genomewide association analysis...
January 2012: American Journal of Clinical Nutrition
Florian Rieder, Philipp Hahn, Lydia Finsterhoelzl, Stephan Schleder, Alexandra Wolf, Andrea Dirmeier, Rocio Lopez, Bo Shen, Gerhard Rogler, Frank Klebl, Thomas Lang
BACKGROUND: We tested a panel of serological anti-glycan antibodies including the novel anti-laminarin (Anti-L) and anti-chitin (Anti-C) antibodies in pediatric Crohn's disease (CD) patients for diagnosis of CD and association with complicated CD behavior. In addition, we compared this panel in pediatric CD with adult CD patients for possible changes in accuracy over time. METHODS: Anti-L, Anti-C, anti-chitobioside (ACCA), anti-laminaribioside (ALCA), anti-mannobioside (AMCA), and anti-Saccaromyces cervisiae (gASCA) antibodies were tested in serum samples of 131 pediatric participants (59 CD, 27 ulcerative colitis [UC], and 45 noninflammatory bowel disease [IBD] controls) with enzyme-linked immunosorbent assay (ELISA)...
July 2012: Inflammatory Bowel Diseases
H N Zerby, J L Bard, S C Loerch, P S Kuber, A E Radunz, F L Fluharty
Two studies were conducted to determine the effects of diet and feed additive on growth and carcass characteristics of lambs and cattle destined for all natural markets. In Exp. 1, 48 Dorset × Hampshire lambs (initial BW 29.4 ± 0.1 kg) were used in a randomized complete block experiment to determine the effects of Aspergillus oryzae extract, Amaferm (AMF) supplementation (1 g/d) in an 85% concentrate diet on growth and carcass characteristics. Lambs were allotted to 12 pens (4 lambs per pen), and blocked by sex and BW...
July 2011: Journal of Animal Science
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