keyword
https://read.qxmd.com/read/38537119/constructing-linear-oriented-pre-vascularized-human-spinal-cord-tissues-for-spinal-cord-injury-repair
#1
JOURNAL ARTICLE
Caixia Fan, Hui Cai, Lulu Zhang, Xianming Wu, Junyan Yan, Lifang Jin, Baowei Hu, Jiaxiong He, Yanyan Chen, Yannan Zhao, Jianwu Dai
Repairing spinal cord injury (SCI) is a global medical challenge lacking effective clinical treatment. Developing human-engineered spinal cord tissues that can replenish lost cells and restore a regenerative microenvironment offers promising potential for SCI therapy. However, creating vascularized human spinal cord-like tissues that mimic the diverse cell types and longitudinal parallel structural features of spinal cord tissues remains a significant hurdle. In present study, vascularized spinal cord tissues (VSCT) were engineered using embryonic human spinal cord-derived neural cells and endothelial cells on linear ordered collagen scaffolds (LOCS)...
March 27, 2024: Advanced Healthcare Materials
https://read.qxmd.com/read/38536913/cardiac-tissue-model-of-immune-induced-dysfunction-reveals-the-role-of-free-mitochondrial-dna-and-the-therapeutic-effects-of-exosomes
#2
JOURNAL ARTICLE
Rick Xing Ze Lu, Naimeh Rafatian, Yimu Zhao, Karl T Wagner, Erika L Beroncal, Bo Li, Carol Lee, Jingan Chen, Eryn Churcher, Daniel Vosoughi, Chuan Liu, Ying Wang, Andrew Baker, Uriel Trahtemberg, Bowen Li, Agostino Pierro, Ana C Andreazza, Claudia C Dos Santos, Milica Radisic
Despite tremendous progress in the development of mature heart-on-a-chip models, human cell-based models of myocardial inflammation are lacking. Here, we bioengineered a vascularized heart-on-a-chip with circulating immune cells to model severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-induced acute myocarditis. We observed hallmarks of coronavirus disease (COVID-19)-induced myocardial inflammation, as the presence of immune cells augmented the secretion of proinflammatory cytokines, triggered progressive impairment of contractile function, and altered intracellular calcium transients...
March 29, 2024: Science Advances
https://read.qxmd.com/read/38536871/species-specific-responses-during-seoul-orthohantavirus-infection-in-human-and-rat-lung-microvascular-endothelial-cells
#3
JOURNAL ARTICLE
Danny Noack, Mirjam C G N van den Hout, Carmen W E Embregts, Wilfred F J van IJcken, Marion P G Koopmans, Barry Rockx
Seoul orthohantavirus (SEOV) is a rat-borne zoonotic virus that is transmitted via inhalation of aerosolized infectious excreta, and can cause hemorrhagic fever with renal syndrome (HFRS) in humans worldwide. In rats, SEOV predominantly exists as a persistent infection in the absence of overt clinical signs. Lack of disease in rats is attributed to downregulation of pro-inflammatory and upregulation of regulatory host responses. As lung microvascular endothelial cells (LMECs) represent a primary target of infection in both human and rats, infections in these cells provide a unique opportunity to study the central role of LMECs in the dichotomy between pathogenicity in both species...
March 27, 2024: PLoS Neglected Tropical Diseases
https://read.qxmd.com/read/38536827/effects-of-aflibercept-and-bevacizumab-on-cell-viability-cell-metabolism-and-inflammation-in-hypoxic-human-m%C3%A3-ller-cells
#4
JOURNAL ARTICLE
Monique Matsuda, Rafael André da Silva, Vinicius Moraes de Paiva Roda, Mônica Valéria Marquezini, Mário Luiz Ribeiro Monteiro, Dânia Emi Hamassaki
Anti-VEGF (vascular endothelial growth factor) drugs such as aflibercept (AFL) and bevacizumab (BVZ) inhibit pathological neo-angiogenesis and vascular permeability in retinal vascular diseases. As cytokines and growth factors are produced by Müller glial cells under stressful and pathological conditions, we evaluated the in vitro effect of AFL (Eylea®, 0.5 mg/mL) and BVZ (Avastin®, 0.5 mg/mL) on cell viability/metabolism, and cytokine/growth factor production by Müller cells (MIO-M1) under cobalt chloride (CoCl2)-induced hypoxia after 24h, 48h and 72h...
2024: PloS One
https://read.qxmd.com/read/38536819/stem-cell-derived-vessels-on-chip-for-cardiovascular-disease-modeling
#5
JOURNAL ARTICLE
Maren Marder, Caroline Remmert, Julius A Perschel, Munkhtur Otgonbayar, Christine von Toerne, Stefanie Hauck, Judith Bushe, Annette Feuchtinger, Bilal Sheikh, Michel Moussus, Matthias Meier
The metabolic syndrome is accompanied by vascular complications. Human in vitro disease models are hence required to better understand vascular dysfunctions and guide clinical therapies. Here, we engineered an open microfluidic vessel-on-chip platform that integrates human pluripotent stem cell-derived endothelial cells (SC-ECs). The open microfluidic design enables seamless integration with state-of-the-art analytical technologies, including single-cell RNA sequencing, proteomics by mass spectrometry, and high-resolution imaging...
March 26, 2024: Cell Reports
https://read.qxmd.com/read/38536816/iqgap1-and-nwasp-promote-human-cancer-cell-dissemination-and-metastasis-by-regulating-%C3%AE-1-integrin-via-fak-and-mrtf-srf
#6
JOURNAL ARTICLE
Camilla Cerutti, Serena Lucotti, Sofia T Menendez, Nicolas Reymond, Ritu Garg, Ignacio A Romero, Ruth Muschel, Anne J Ridley
Attachment of circulating tumor cells to the endothelial cells (ECs) lining blood vessels is a critical step in cancer metastatic colonization, which leads to metastatic outgrowth. Breast and prostate cancers are common malignancies in women and men, respectively. Here, we observe that β1-integrin is required for human prostate and breast cancer cell adhesion to ECs under shear-stress conditions in vitro and to lung blood vessel ECs in vivo. We identify IQGAP1 and neural Wiskott-Aldrich syndrome protein (NWASP) as regulators of β1-integrin transcription and protein expression in prostate and breast cancer cells...
March 26, 2024: Cell Reports
https://read.qxmd.com/read/38536659/sorcin-promotes-proliferation-of-hepatocellular-carcinoma-by-regulating-vegfa-b-via-pi3k-pathway
#7
JOURNAL ARTICLE
Huan Zhang, Shanshan Hu, Jaceline Gislaine Pires Sanches, Yizi Li, Yuanyi Wei, Chunwen Pu, Jun Zhang
Hepatocellular carcinoma (HCC) is a highly vascularized tumor, one of the most common and lethal cancer-related tumor deaths worldwide, with cell proliferation playing a key role. In this study our western blot results and data from TAGC demonstrate a strong association between Sorcin (SRI) overexpression and poor outcomes in HCC. Moreover, SRI overexpression was remarkably effective in promoting proliferation in vitro and increasing tumor growth in vivo, which were attenuated by knocking down SRI. Mechanistically, SRI regulated vascular endothelial growth factor A (VEGFA) and vascular endothelial growth factor B (VEGFB) through PI3K/Akt/FOXO1 signal pathway...
March 27, 2024: Journal of Physiology and Biochemistry
https://read.qxmd.com/read/38536197/adamts5-promotes-permeability-of-the-blood-brain-barrier-during-treponema-pallidum-subspecies-pallidum-invading-the-central-nervous-system
#8
JOURNAL ARTICLE
Zuoxi Chen, Fangzhi Du, Ruihua Zhang, Qingyun Wu, Zhiyu Lu, Rui-Li Zhang, Qianqiu Wang
The pathogenesis of neurosyphilis remains unclear. A previous study found a noteworthy up-regulation of a disintegrin and metalloproteinase with thrombospondin type 1 motif 5 (ADAMTS5) gene in human brain microvascular endothelial cells cocultured with Treponema pallidum subspecies pallidum (Tp). To investigate the ADAMTS5 role in Tp invading the central nervous system (CNS), we conducted relevant experiments. Our study revealed that Tp caused an increase in human cortical microvascular endothelial cell/D3 (hCMEC/D3) barrier permeability and significantly enhanced ADAMTS5 expression...
March 27, 2024: ACS Infectious Diseases
https://read.qxmd.com/read/38535980/humanin-inhibits-lymphatic-endothelial-cells-dysfunction-to-alleviate-myocardial-infarction-reperfusion-injury-via-bnip3-mediated-mitophagy
#9
JOURNAL ARTICLE
Lu Chen, Xiaohua Yang, Kai Wang, Lina Guo, Cao Zou
OBJECTIVE: Acute myocardial infarction (AMI) ranks among the top contributors to sudden death and disability worldwide. It should be noted that current therapies always cause increased reperfusion damage. Evidence suggests that humanin (HN) reduces mitochondrial dysfunction to have cardio-protective effects against MI-reperfusion injury. In this context, we hypothesized that HN may attenuate MI-reperfusion injury by alleviating lymphatic endothelial cells dysfunction through the regulation of mitophagy...
March 27, 2024: Free Radical Research
https://read.qxmd.com/read/38535794/the-role-of-snake-venom-disintegrins-in-angiogenesis
#10
REVIEW
Patricia Bianca Clissa, Maisa Splendore Della-Casa, Bianca Cestari Zychar, Sabri Saeed Sanabani
Angiogenesis, the formation of new blood vessels, plays a critical role in various physiological and pathological conditions. Snake venom disintegrins (SVDs) have been identified as significant regulators of this process. In this review, we explore the dual roles of SVD in angiogenesis, both as antiangiogenic agents by inhibiting integrin binding and interfering with vascular endothelial growth factors and as proangiogenic agents by enhancing integrin binding, stimulating cell migration and proliferation, and inducing neoangiogenesis...
March 1, 2024: Toxins
https://read.qxmd.com/read/38535583/viral-infections-are-they-a-trigger-and-risk-factor-of-alzheimer-s-disease
#11
REVIEW
Meagan D Rippee-Brooks, Wenzhe Wu, Jianli Dong, Miguel Pappolla, Xiang Fang, Xiaoyong Bao
Alzheimer's Disease (AD), a progressive and debilitating condition, is reported to be the most common type of dementia, with at least 55 million people believed to be currently affected. Many causation hypotheses of AD exist, yet the intriguing link between viral infection and its possible contribution to the known etiology of AD has become an attractive focal point of research for the field and a challenging study task. In this review, we will explore the historical perspective and milestones that led the field to investigate the viral connection to AD...
March 8, 2024: Pathogens
https://read.qxmd.com/read/38535495/spironolactone-induces-vasodilation-by-endothelium-dependent-mechanisms-involving-no-and-by-endothelium-independent-mechanisms-blocking-ca-2-channels
#12
JOURNAL ARTICLE
Margarida Lorigo, João Amaro, Elisa Cairrao
BACKGROUND: Spironolactone (SPI) is a diuretic widely used to treat cardiovascular diseases (CVD) and is non-specific for mineralocorticoid receptors (MR) and with an affinity for progesterone (PR) and androgen (AR) receptors. Since 2009, it has been suggested that pharmaceuticals are emerging contaminants (called EDC), and recently, it was reported that most EDC are AR and MR antagonists and estrogen receptors (ER) agonists. Concerning SPI, endocrine-disrupting effects were observed in female western mosquitofish, but there are still no data regarding the SPI effects as a possible human EDC...
March 1, 2024: Journal of Xenobiotics
https://read.qxmd.com/read/38535082/comparison-of-tissue-factors-in-the-ontogenetic-aspects-of-human-cholesteatoma
#13
JOURNAL ARTICLE
Kristaps Dambergs, Gunta Sumeraga, Māra Pilmane
BACKGROUND: An acquired cholesteatoma is a benign but locally aggressive lesion in the middle ear. It is characterized by chronic inflammation and the destruction of surrounding bone. Therefore, the aim of this study was to compare defensins HβD-2 and HβD-4; pro- and anti-inflammatory cytokines IL-1α and IL-10; proliferation marker Ki-67; transcription factor NF-κβ; angiogenetic factor VEGF; Sonic hedgehog gene protein SHH; and remodeling factors MMP-2, MMP-9, TIMP-2, and TIMP-4 in adult and pediatric cholesteatoma tissue, and to compare these groups with control skin tissue...
March 21, 2024: Diagnostics
https://read.qxmd.com/read/38534884/factor-vii-deficiency-in-systemic-mastocytosis-with-an-associated-myeloid-neoplasm
#14
Giorgio Rosati, Sofia Camerlo, Alessandro Fornari, Valerio Marci, Barbara Montaruli, Alessandro Morotti
Factor VII (FVII) deficiency is a rare bleeding disorder that can be classified as congenital or acquired, and the majority of acquired cases are due to vitamin K deficiency or liver disease. Isolated acquired FVII deficiency is a rare occurrence and has been associated with inhibitors or auto-antibodies. Here, we describe a patient with polycythemia vera who developed systemic mastocytosis and FVII deficiency simultaneously. FVII deficiency was not caused by inhibitors and improved with antineoplastic treatment...
March 12, 2024: Hematology Reports
https://read.qxmd.com/read/38534770/the-combination-of-oolonghomobisflavan-b-and-diallyl-disulfide-induces-apoptotic-cell-death-via-67-kda-laminin-receptor-cyclic-guanosine-monophosphate-in-acute-myeloid-leukemia-cells
#15
JOURNAL ARTICLE
Jaehoon Bae, Su-Jin Park
Diallyl disulfide (DADS) is a well-known principal functional component derived from garlic ( Allium sativum ) that has various health benefits. Previously, we identified a 67-kDa laminin receptor, a receptor for oolong tea polyphenol oolonghomobisflavan B (OHBFB). However, its molecular mechanisms still remain to be elucidated. Here, we show that DADS synergistically enhanced the effect of the oolong tea polyphenol oolonghomobisflavan B (OHBFB), which induces apoptosis in acute myeloid leukemia (AML) cancer cells without affecting normal human peripheral blood mononuclear cells (PBMCs)...
March 14, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38534600/fibrin-based-hydrogels-with-reactive-amphiphilic-copolymers-for-mechanical-adjustments-allow-for-capillary-formation-in-2d-and-3d-environments
#16
JOURNAL ARTICLE
Svenja Wein, Carina Schemmer, Miriam Aischa Al Enezy-Ulbrich, Shannon Anna Jung, Stephan Rütten, Mark Kühnel, Danny Jonigk, Wilhelm Jahnen-Dechent, Andrij Pich, Sabine Neuss
This study focuses on enhancing controllable fibrin-based hydrogels for tissue engineering, addressing existing weaknesses. By integrating a novel copolymer, we improved the foundation for cell-based angiogenesis with adaptable structural features. Tissue engineering often faces challenges like waste disposal and nutrient supply beyond the 200 µm diffusion limit. Angiogenesis breaks through this limitation, allowing the construction of larger constructs. Our innovative scaffold combination significantly boosts angiogenesis, resulting in longer branches and more capillary network junctions...
March 6, 2024: Gels
https://read.qxmd.com/read/38534576/hydrogel-based-therapy-for-age-related-macular-degeneration-current-innovations-impediments-and-future-perspectives
#17
REVIEW
Chengzhi Zhang, Jiale Wang, Hao Wu, Wenhui Fan, Siyu Li, Dong Wei, Zongming Song, Ye Tao
Age-related macular degeneration (AMD) is an ocular disease that leads to progressive photoreceptor death and visual impairment. Currently, the most common therapeutic strategy is to deliver anti-vascular endothelial growth factor (anti-VEGF) agents into the eyes of patients with wet AMD. However, this treatment method requires repeated injections, which potentially results in surgical complications and unwanted side effects for patients. An effective therapeutic approach for dry AMD also remains elusive. Therefore, there is a surge of enthusiasm for the developing the biodegradable drug delivery systems with sustained release capability and develop a promising therapeutic strategy...
February 21, 2024: Gels
https://read.qxmd.com/read/38534545/applications-of-imaging-technologies-in-fuchs-endothelial-corneal-dystrophy-a-narrative-literature-review
#18
REVIEW
Sang Beom Han, Yu-Chi Liu, Chang Liu, Jodhbir S Mehta
Fuchs endothelial corneal dystrophy (FECD) is a complex genetic disorder characterized by the slow and progressive degeneration of corneal endothelial cells. Thus, it may result in corneal endothelial decompensation and irreversible corneal edema. Moreover, FECD is associated with alterations in all corneal layers, such as thickening of the Descemet membrane, stromal scarring, subepithelial fibrosis, and the formation of epithelial bullae. Hence, anterior segment imaging devices that enable precise measurement of functional and anatomical changes in the cornea are essential for the management of FECD...
March 11, 2024: Bioengineering
https://read.qxmd.com/read/38534529/femtosecond-laser-cutting-of-human-crystalline-lens-capsule-and-decellularization-for-corneal-endothelial-bioengineering
#19
JOURNAL ARTICLE
Olfa Ben Moussa, Louise Parveau, Inès Aouimeur, Grégory Egaud, Corantin Maurin, Sofiane Fraine, Sébastien Urbaniak, Chantal Perrache, Zhiguo He, Sedao Xxx, Oliver Dorado Cortez, Sylvain Poinard, Cyril Mauclair, Philippe Gain, Gilles Thuret
The bioengineering of corneal endothelial grafts consists of seeding in vitro cultured corneal endothelial cells onto a thin, transparent, biocompatible, and sufficiently robust carrier which can withstand surgical manipulations. This is one of the most realistic alternatives to donor corneas, which are in chronic global shortage. The anterior capsule of the crystalline lens has already been identified as one of the best possible carriers, but its challenging manual preparation has limited its use. In this study, we describe a femtosecond laser cutting process of the anterior capsule of whole lenses in order to obtain capsule discs of 8 mm diameter, similar to conventional endothelial grafts...
March 5, 2024: Bioengineering
https://read.qxmd.com/read/38534477/carotid-artery-bypass-surgery-of-in-body-tissue-architecture-induced-small-diameter-biotube-in-a-goat-model-a-pilot-study
#20
JOURNAL ARTICLE
Tadashi Umeno, Kazuki Mori, Ryosuke Iwai, Takayuki Kawashima, Takashi Shuto, Yumiko Nakashima, Tsutomu Tajikawa, Yasuhide Nakayama, Shinji Miyamoto
Biotubes are autologous tubular tissues developed within a patient's body through in-body tissue architecture, and they demonstrate high potential for early clinical application as a vascular replacement. In this pilot study, we used large animals to perform implantation experiments in preparation for preclinical testing of Biotube. The biological response after Biotube implantation was histologically evaluated. The designed Biotubes (length: 50 cm, internal diameter: 4 mm, and wall thickness: 0.85 mm) were obtained by embedding molds on the backs of six goats for a predetermined period (1-5 months)...
February 21, 2024: Bioengineering
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