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https://www.readbyqxmd.com/read/27915436/bone-marrow-produces-sufficient-alloreactive-natural-killer-nk-cells-in-vivo-to-cure-mice-from-subcutaneously-and-intravascularly-injected-4t1-breast-cancer
#1
Michel van Gelder, Ariane Vanclée, Catharina H M J van Elssen, Pierre Hupperets, Lotte Wieten, Gerard M Bos
PURPOSE: Administration of 5 million alloreactive natural killer (NK) cells after low-dose chemo-irradiation cured mice of 4T1 breast cancer, supposedly dose dependent. We now explored the efficacy of bone marrow as alternative in vivo source of NK cells for anti-breast cancer treatment, as methods for in vitro clinical scale NK cell expansion are still in developmental phases. METHODS: Progression-free survival (PFS) after treatment with different doses of spleen-derived alloreactive NK cells to 4T1-bearing Balb/c mice was measured to determine a dose-response relation...
December 3, 2016: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/27915415/exendin-4-protects-huvecs-from-tunicamycin-induced-apoptosis-via-inhibiting-the-ire1a-jnk-caspase-3-pathway
#2
Li Wu, XiaoYing Liu, LinXi Wang, YanPing Wang, LiJing Wang, BinBin Guan, Zhou Chen, LiBin Liu
PURPOSE: The abnormal increase of apoptosis of endothelial cells induced by endoplasmic reticulum stress is a significant factor for vascular disease, especially for atherosclerosis. Protecting endothelial cells from endoplasmic reticulum stress is a crucial strategies to combate these diseases. The goal of this study was to explore the effect of Exendin-4, a glucagon-like peptide-1 receptor agonist, on tunicamycin-induced apoptosis in human umbilical vein endothelial cells. METHODS: All studies were performed in primary human umbilical vein endothelial cells treated with tunicamycin with or without Exendin-4 pretreatment...
December 3, 2016: Endocrine
https://www.readbyqxmd.com/read/27915381/regulation-of-non-homologous-end-joining-via-post-translational-modifications-of-components-of-the-ligation-step
#3
REVIEW
Kristína Durdíková, Miroslav Chovanec
DNA double-strand breaks are the most serious type of DNA damage and non-homologous end joining (NHEJ) is an important pathway for their repair. In Saccharomyces cerevisiae, three complexes mediate the canonical NHEJ pathway, Ku (Ku70/Ku80), MRX (Mre11/Rad50/Xrs2) and DNA ligase IV (Dnl4/Lif1). Mammalian NHEJ is more complex, primarily as a consequence of the fact that more factors are involved in the process, and also because higher chromatin organization and more complex regulatory networks exist in mammals...
December 3, 2016: Current Genetics
https://www.readbyqxmd.com/read/27915380/proteins-interacting-with-crea-and-creb-in-the-carbon-catabolite-repression-network-in-aspergillus-nidulans
#4
Md Ashiqul Alam, Joan M Kelly
In Aspergillus nidulans, carbon catabolite repression (CCR) is mediated by the global repressor protein CreA. The deubiquitinating enzyme CreB is a component of the CCR network. Genetic interaction was confirmed using a strain containing complete loss-of-function alleles of both creA and creB. No direct physical interaction was identified between tagged versions of CreA and CreB. To identify any possible protein(s) that may form a bridge between CreA and CreB, we purified both proteins from mycelia grown in media that result in repression or derepression...
December 3, 2016: Current Genetics
https://www.readbyqxmd.com/read/27915346/mir-181a-targets-phlpp2-to-augment-akt-signaling-and-regulate-proliferation-and-apoptosis-in-human-keloid-fibroblasts
#5
Zhen Rang, Zong-Yang Wang, Qiu-Yu Pang, You-Wei Wang, Ge Yang, Fan Cui
BACKGROUND/AIMS: Keloids are fibrous overgrowths induced by cutaneous injury. MicroRNAs (miRNAs) have recently emerged as post-transcriptional gene repressors and participants in a diverse array of pathophysiological processes leading to skin disease. The purpose of the current study was to explore the precise functions of miR-181a in human keloid development and the underlying mechanisms. METHODS: A miRNA microarray analysis was performed to compare expression profiles between keloid and normal skin tissues...
December 5, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27915333/prss8-is-downregulated-and-suppresses-tumour-growth-and-metastases-in-hepatocellular-carcinoma
#6
Li Zhang, Guozhan Jia, Binya Shi, Guanqun Ge, Hongbin Duan, Yunsheng Yang
BACKGROUND: Protease serine 8 (PRSS8), a trypsin-like serine peptidase, has been shown to function as a tumour suppressor in various malignancies. The present study aimed to investigate the expression pattern, prognostic value and the biological role of PRSS8 in human hepatocellular carcinoma (HCC). METHODS: PRSS8 expression in 106 HCC surgical specimens was examined by Real-time polymerase chain reaction (PCR) and immunohistochemistry, and its clinical significance was analysed...
December 5, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27915294/insightful-directed-evolution-of-escherichia-coli-quorum-sensing-promoter-region-of-the-lsracdbfg-operon-a-tool-for-synthetic-biology-systems-and-protein-expression
#7
Pricila Hauk, Kristina Stephens, Ryan Mckay, Chelsea Ryan Virgile, Hana Ueda, Marc Ostermeier, Kyoung-Seok Ryu, Herman O Sintim, William E Bentley
Quorum sensing (QS) regulates many natural phenotypes (e.q. virulence, biofilm formation, antibiotic resistance), and its components, when incorporated into synthetic genetic circuits, enable user-directed phenotypes. We created a library of Escherichia coli lsr operon promoters using error-prone PCR (ePCR) and selected for promoters that provided E. coli with higher tetracycline resistance over the native promoter when placed upstream of the tet(C) gene. Among the fourteen clones identified, we found several mutations in the binding sites of QS repressor, LsrR...
December 1, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27915292/the-dual-role-of-dksa-protein-in-the-regulation-of-escherichia-coli-pargx-promoter
#8
Robert Łyżeń, Amarnath Maitra, Klaudia Milewska, Maja Kochanowska-Łyżeń, V James Hernandez, Agnieszka Szalewska-Pałasz
Gene expression regulation by the stringent response effector, ppGpp, is facilitated by DksA protein; however DksA and ppGpp can play independent roles in transcription. In Escherichia coli, the pArgX promoter which initiates the transcription of four tRNA genes was shown to be inhibited by ppGpp. Our studies on the role of DksA in pArgX regulation revealed that it can stimulate transcription by increasing the binding of RNA polymerase to the promoter and the productive transcription complex formation. However, when DksA is present together with ppGpp a severe down-regulation of promoter activity is observed...
December 1, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27915283/distributions-and-relationships-of-virio-and-pico-plankton-in-the-epi-meso-and-bathy-pelagic-zones-of-the-western-pacific-ocean
#9
Yantao Liang, Yongyu Zhang, Yao Zhang, Tingwei Luo, Richard B Rivkin, Nianzhi Jiao
Virio- and pico-plankton mediate important biogeochemical processes and the environmental factors which regulates their dynamics and the virus-host interactions are incompletely known, especially in the deep sea. Here we report on their distributions and relationships with environmental factors at 21 stations covering a latitudinal range (2-23(o) N) in the Western Pacific Ocean. This region is characterized by a complex western boundary current system. Synechococcus, autotrophic picoeukaryotes, heterotrophic prokaryotes and virus-like particles (VLPs) were high (<2...
December 2, 2016: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/27915251/identification-of-a-campylobacter-coli-methyltransferase-targeting-adenines-at-gatc-sites
#10
Vikrant Dutta, Eric Altermann, Maria D Crespo, Jonathan W Olson, Robin M Siletzky, Sophia Kathariou
Campylobacter coli can infect humans and colonize multiple other animals but its host-associated genes or adaptations are poorly understood. Adenine methylation at GATC sites, resulting in MboI resistance of genomic DNA, was earlier frequently detected among C. coli from swine but not among turkey-derived isolates. The underlying genetic basis has remained unknown. Comparative genome sequence analyses of C. coli 6461, a swine-derived strain with MboI-resistant DNA, revealed two chromosomal ORFs, 0059 and 0060, encoding a putative DNA methyltransferase and a conserved hypothetical protein, respectively, which were lacking from the genome of the turkey-derived C...
December 2, 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27915246/neutrophil-dependent-pentraxin-3-and-reactive-oxygen-species-production-modulate-endothelial-dysfunction-in-haemodialysis-patients
#11
Maria Pia dell'Oglio, Simona Simone, Marco Ciccone, Roberto Corciulo, Michele Gesualdo, Annapaola Zito, Francesca Cortese, Giuseppe Castellano, Margherita Gigante, Loreto Gesualdo, Giuseppe Grandaliano, Giovanni Battista Pertosa
BACKGROUND: The aim of this study was to investigate neutrophil activation and its role in long pentraxin-3 (PTX3) release and oxidative stress generation during haemodialysis (HD) and to correlate neutrophil PTX3 and oxidant expression with endothelial dysfunction. METHODS: Forty-seven uraemic patients on stable HD, 12 healthy subjects and 15 patients with congestive heart failure (New York Heart Association classes III and IV) were enrolled. Neutrophil PTX3 protein expression was evaluated by confocal microscopy...
December 3, 2016: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/27915243/signaling-pathways-of-replication-stress-in-yeast
#12
Benjamin Pardo, Laure Crabbé, Philippe Pasero
Eukaryotic cells activate the S-phase checkpoint in response to a variety of events affecting the progression of replication forks, collectively referred to as replication stress. This signaling pathway is divided in two branches: the DNA damage checkpoint (DDC) and the DNA replication checkpoint (DRC). Both pathways are activated by the sensor kinase Mec1 and converge on the effector kinase Rad53. However, the DDC operates throughout the cell cycle and depends on the checkpoint mediator Rad9 to activate Rad53, whereas the DRC is specific to S phase and is mediated by Mrc1 and other fork components to signal replication impediments...
December 2, 2016: FEMS Yeast Research
https://www.readbyqxmd.com/read/27915242/distinct-mechanisms-of-mutant-huntingtin-toxicity-in-different-yeast-strains
#13
Genrikh V Serpionov, Alexander I Alexandrov, Michael D Ter-Avanesyan
Expansion of polyglutamine stretches in several proteins cause neurodegenerative amyloidoses, including Huntington disease. In yeast, mutant huntingtin (mHtt) with a stretch of 103 glutamine residues (HttQ103) forms toxic aggregates. A range of yeast strains have been used to elucidate the mechanisms of mHtt toxicity, and have revealed perturbations of various unrelated processes. HttQ103 aggregates can induce aggregation of cellular proteins, many of which contain glutamine/asparagine-rich regions, including Sup35 and Def1...
December 2, 2016: FEMS Yeast Research
https://www.readbyqxmd.com/read/27915233/molecular-basis-for-the-autonomous-promotion-of-cell-proliferation-by-angiogenin
#14
Trish T Hoang, Ronald T Raines
Canonical growth factors act indirectly via receptor-mediated signal transduction pathways. Here, we report on an autonomous pathway in which a growth factor is internalized, has its localization regulated by phosphorylation, and ultimately uses intrinsic catalytic activity to effect epigenetic change. Angiogenin (ANG), a secreted vertebrate ribonuclease, is known to promote cell proliferation, leading to neovascularization as well as neuroprotection in mammals. Upon entering cells, ANG encounters the cytosolic ribonuclease inhibitor protein, which binds with femtomolar affinity...
December 2, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27915214/recent-advances-in-expanding-the-coverage-of-the-lipidome
#15
REVIEW
Sergey Tumanov, Jurre J Kamphorst
The lipidome comprises a large array of molecules with diverse physicochemical properties. Lipids are structural components of cells, act as a source of energy, and function as signaling mediators. Alterations in lipid metabolism are involved in the onset and progression of a variety of diseases, including metabolic syndrome and cancer. Because of this, interest in lipidomics, the comprehensive characterization of the lipidome by mass spectrometry, has intensified in recent years. However, obtaining a truly complete overview of all lipids in a sample has remained very challenging due to their enormous structural diversity...
December 1, 2016: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/27915200/effects-of-prophylactic-indomethacin-on-vasopressor-dependent-hypotension-in-extremely-preterm-infants
#16
Melissa Liebowitz, Jane Koo, Andrea Wickremasinghe, Isabel Elaine Allen, Ronald I Clyman
OBJECTIVE: To determine whether a moderate-to-large patent ductus arteriosus (PDA) is responsible for vasopressor-dependent hypotension, occurring at the end of the first postnatal week. STUDY DESIGN: We performed a retrospective, double cohort controlled study of infants delivered at ≤27(+6) weeks' gestation (n = 313). From January 2004 through April 2011, all infants were treated with prophylactic indomethacin ([PINDO] epoch). From May 2011 through December 2015, no infant was treated with indomethacin until at least 8 postnatal days (conservative epoch)...
December 1, 2016: Journal of Pediatrics
https://www.readbyqxmd.com/read/27915178/anticancer-copper-pyridine-benzimidazole-complexes-ros-generation-biomolecule-interactions-and-cytotoxicity
#17
Kathleen E Prosser, Stephanie W Chang, Felix Saraci, Phuc H Le, Charles J Walsby
The Cu(II) complex CuCl2(pbzH), pbzH=2-(2-pyridyl)benzimidazole, and derivatives modified at the non-coordinated nitrogen of the benzimidazole fragment, have been studied as anticancer agents. These compounds show promising cytotoxicity against A549 adenocarcinomic alveolar basal epithelial cells with IC50 values in the range of 5-10μM. Importantly, this activity is higher than either CuCl2·2H2O or the individual ligands, demonstrating that ligand coordination to the Cu(II) centres of the complexes is required for full activity...
November 5, 2016: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/27915176/mining-identification-and-function-analysis-of-micrornas-and-target-genes-in-peanut-arachis-hypogaea-l
#18
Tingting Zhang, Shuhao Hu, Caixia Yan, Chunjuan Li, Xiaobo Zhao, Shubo Wan, Shihua Shan
In the present investigation, a total of 60 conserved peanut (Arachis hypogaea L.) microRNA (miRNA) sequences, belonging to 16 families, were identified using bioinformatics methods. There were 392 target gene sequences, identified from 58 miRNAs with Target-align software and BLASTx analyses. Gene Ontology (GO) functional analysis suggested that these target genes were involved in mediating peanut growth and development, signal transduction and stress resistance. There were 55 miRNA sequences, verified employing a poly (A) tailing test, with a success rate of up to 91...
November 24, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27915166/-i-can-t-accept-that-feeling-relationships-between-interoceptive-awareness-mindfulness-and-eating-disorder-symptoms-in-females-with-and-at-risk-of-an-eating-disorder
#19
Paul Lattimore, Bethan R Mead, Leanne Irwin, Lorna Grice, Ruth Carson, Peter Malinowski
Mindfulness based therapies (MBTs) for eating disorders show potential benefit for outcomes yet evidence is scarce regarding the mechanisms by which they influence remission from symptoms. One way that mindfulness approaches create positive outcomes is through enhancement of emotion regulation skills. Maladaptive emotion regulation is a key psychological feature of all eating disorders. The aim of the current study was to identify facets of emotion regulation involved in the relationship between mindfulness and maladaptive eating behaviours...
November 16, 2016: Psychiatry Research
https://www.readbyqxmd.com/read/27915160/histone-deacetylase-9-plays-a-role-in-the-antifibrogenic-effect-of-astaxanthin-in-hepatic-stellate-cells
#20
Yue Yang, Minkyung Bae, Young-Ki Park, Yoojin Lee, Tho X Pham, Swetha Rudraiah, José Manautou, Sung I Koo, Ji-Young Lee
Activation of hepatic stellate cells (HSCs) is critical for liver fibrosis development. Previously, we showed that astaxanthin (ASTX), a xanthophyll carotenoid, has antifibrogenic effects in LX-2 cells, a human HSC cell line. We sought to determine the effect of ASTX on HSC activation, and to identify molecular mediators that are critically involved in the processes. ASTX prevented the activation of mouse primary HSCs, as evidenced by attenuated induction of procollagen type I α1. In human primary HSCs, ASTX also inhibited transforming growth factor β1 (TGFβ1)-induced fibrogenic gene expression...
November 12, 2016: Journal of Nutritional Biochemistry
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