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fosfomycin resistance

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https://www.readbyqxmd.com/read/29137487/emergence-of-fosfomycin-resistance-among-isolates-of-escherichia-coli-harboring-extended-spectrum-and-ampc-%C3%AE-lactamases
#1
Aghil Bahramian, Gita Eslami, Ali Hashemi, Ali Tabibi, Mohsen Heidary
Urinary tract infection (UTI) is a common type of infectious disease globally. The aim of this study was to detect the frequency of fosA3 and fosC2 genes in extended-spectrum β-lactamases (ESBL) and blaDHA, blaCMY-2, and blaCMY-42 genes in AmpC β-lactamases-producing isolates of Escherichia coli. In total, 120 isolates of E. coli were collected from three teaching hospitals between March 2014 and February 2015. Antibiotic susceptibility tests were carried out by disk diffusion method. The presence of blaCMY-2, blaCMY-42, blaDHA, fosA3, and fosC2 genes was detected by polymerase chain reaction (PCR) and sequencing...
November 15, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/29124451/current-treatment-concepts-for-iatrogenic-ventriculitis-a-nationwide-survey-in-germany
#2
Niklas von Spreckelsen, Norma Jung, Sergej Telentschak, Jürgen Hampl, Roland Goldbrunner, Stefan Grau
BACKGROUND: Iatrogenic ventriculitis is a common complication of the external ventricular drainage. While the procedure and indications for external ventricular drains (EVD) are highly standardized, the treatment of ventriculitis is not clearly defined. OBJECTIVE: To depict the treatment of iatrogenic ventriculitis currently performed in German hospitals. METHODS: A standardized questionnaire consisting of 18 multiple choice questions, each with the ability to provide additional individual answers, covering the diagnosis and treatment of iatrogenic ventriculitis as well as general handling of EVDs, was sent to 121 neurosurgical hospitals registered in the German Society for Neurosurgery (DGNC)...
November 9, 2017: Acta Neurochirurgica
https://www.readbyqxmd.com/read/29122729/draft-genome-sequence-of-enterobacter-cloacae-st128-a-clinical-strain-coproducing-kpc-2-and-ndm-1-carbapenemases
#3
Xi Li, Yongze Zhu, Mengyuan Shen, Jing Du, Lei Zhang, Dairong Wang
OBJECTIVES: Enterobacter cloacae is one of the major pathogens responsible for a variety of human infections. Here, we report the draft genome sequence of a multidrug-resistant E. cloacae strain HBY that was isolated from a female in China. METHODS: Whole-genomic DNA of E. cloacae strain HBY was extracted and was sequenced using an Illumina-Hiseq™ 2000 platform. The generated sequence reads were assembled using CLC Genomics Workbench. The draft genome was annotated using Rapid Annotation using Subsystem Technology and the presence of antimicrobial resistance genes was identified...
November 6, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29109580/fosfomycin-susceptibility-in-multidrug-resistant-enterobacteriaceae-species-and-vancomycin-resistant-enterococci-urinary-isolates
#4
Linda B Ou, Lynn Nadeau
Background: Broad-spectrum antibiotics are often used to treat urinary tract infections (UTIs) due to drug-resistant species of Enterobacteriaceae and Enterococcus (e.g., organisms producing extended-spectrum β-lactamase [ESBL] or AmpC β-lactamase, as well as vancomycin-resistant enterococci [VRE]). However, this type of therapy can promote selection of resistant organisms and may necessitate venous access. Fosfomycin is an orally administered, single-dose antibiotic for the treatment of uncomplicated UTI...
September 2017: Canadian Journal of Hospital Pharmacy
https://www.readbyqxmd.com/read/29106538/origin-of-the-plasmid-mediated-fosfomycin-resistance-gene-fosa3
#5
Ryota Ito, Marissa P Pacey, Roberta T Mettus, Nicolas Sluis-Cremer, Yohei Doi
Background: fosA3 is the most commonly reported plasmid-mediated fosfomycin resistance gene among Enterobacteriaceae. Objectives: To identify the origin of fosA3. Methods: The chromosome of Kluyvera georgiana clinical strain YDC799 was fully sequenced with single-molecule real-time sequencing. Comparative genetic analysis was performed for K. georgiana YDC799, K. georgiana type strain ATCC 51603 and representative fosA3-carrying plasmids. fosA genes were cloned in Escherichia coli to confirm function...
November 2, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29102775/blandm-1-producing-multidrug-resistant-escherichia-coli-isolated-from-a-companion-dog-in-china
#6
Lanqing Cui, Lei Lei, Yuan Lv, Rongmin Zhang, Xiaoyu Liu, Mei Li, Fan Zhang, Yang Wang
OBJECTIVES: This sudy aimed to characterize a blaNDM-1-producing Escherichia coli from a companion dog. METHODS: This strain was isolated from a surveillance study of carbapenem-resistant Gram-negative bacteria and the specie was identified by API 20E system and MALDI-TOF MS. MICs of various antimicrobial agents were determined by agar dilution. Multilocus sequence typing (MLST) and various carbapenemase genes including blaNDM, blaIMP, blaVIM, blaSME, blaIMI, blaKPC, blaGES, blaSPM, blaGIM, blaNMC, blaDIM, blaSIM, blaOXA-23, blaOXA-24, blaOXA-48, blaOXA-51 and blaOXA-58 were screened by PCR...
November 1, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29093108/frequency-and-mechanisms-of-spontaneous-fosfomycin-non-susceptibility-observed-upon-disk-diffusion-testing-of-escherichia-coli
#7
Aaron Lucas, Ryota Ito, Mustapha M Mustapha, Christi L McElheny, Roberta Mettus, Sarah L Bowler, Serena F Kantz, Marissa P Pacey, A William Pasculle, Vaughn S Cooper, Yohei Doi
Fosfomycin maintains activity against most Escherichia coli clinical isolates, but growth of E. coli colonies within the zone of inhibition around the fosfomycin disk is occasionally observed upon susceptibility testing. We aimed to estimate the frequency of such non-susceptible inner colony mutants and identify the underlying resistance mechanisms. Disk diffusion testing of fosfomycin was performed on 649 multidrug-resistant E. coli clinical isolates collected between 2011 and 2015. For those producing inner colonies inside the susceptible range, the parental strains and their representative inner colony mutants were subjected to MIC testing, whole genome sequencing, qRT-PCR, and carbohydrate utilization studies...
November 1, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29091221/screening-for-synergistic-activity-of-antimicrobial-combinations-against-carbapenem-resistant-enterobacteriaceae-using-inkjet-printer-based-technology
#8
Thea Brennan-Krohn, Katherine A Truelson, Kenneth P Smith, James E Kirby
Background: Synergistic combination antimicrobial therapy may provide new options for treatment of MDR infections. However, comprehensive in vitro synergy data are limited and facile methods to perform synergy testing in a clinically actionable time frame are unavailable. Objectives: To systematically investigate a broad range of antibiotic combinations for evidence of synergistic activity against a collection of carbapenem-resistant Enterobacteriaceae (CRE) isolates...
October 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29091201/is26-mediated-formation-of-a-virulence-and-resistance-plasmid-in-salmonella-enteritidis
#9
Marcus Ho-Yin Wong, Edward Wai-Chi Chan, Sheng Chen
Objectives: To characterize a novel virulence-resistance plasmid pSE380T carried by a Salmonella enterica serotype Enteritidis clinical strain SE380. Methods: The plasmid pSE380T was conjugated to Escherichia coli strain J53 and sequenced by PacBio RSII, followed by subsequent annotation and genetic analysis. Results: Sequence analysis of this plasmid revealed that the entire Salmonella Enteritidis-specific virulence plasmid, pSEN, had been incorporated into an IncHI2 MDR plasmid, which comprises the cephalosporin and fosfomycin resistance determinants blaCTX-M-14 and fosA3...
October 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29079617/staphylococcus-edaphicus-sp-nov-isolated-in-antarctica-harbours-mecc-gene-and-genomic-islands-with-suspected-role-in-adaptation-to-extreme-environment
#10
Roman Pantůček, Ivo Sedláček, Adéla Indráková, Veronika Vrbovská, Ivana Mašlaňová, Vojtěch Kovařovic, Pavel Švec, Stanislava Králová, Lucie Krištofová, Jana Kekláková, Petr Petráš, Jiří Doškař
Two Gram-stain-positive, coagulase-negative staphylococcal strains were isolated from abiotic sources, stone fragments and sandy soil in James Ross Island, Antarctica. Here we describe properties of novel species of the genus Staphylococcus that has near identical 16S rRNA gene sequence to Staphylococcus saprophyticus However, compared to S. saprophyticus and the next closest relatives, the new species demonstrates considerable phylogenetic distance at whole genome level, average nucleotide identity <85 %, and inferred DNA-DNA hybridization <30%...
October 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29064348/the-activity-of-fosfomycin-against-extended-spectrum-beta-lactamase-producing-isolates-of-enterobacteriaceae-recovered-from-urinary-tract-infections-a-single-center-study-over-a-period-of-12-years
#11
Parisa Aris, Mohammad Ali Boroumand, Mohammad Rahbar, Masoumeh Douraghi
Despite global efforts to tackle resistance in extended-spectrum beta-lactamase (ESBL)-producing isolates via old antibiotics, there are limited data on the efficacy of fosfomycin-an old oral antibiotic-against Enterobacteriaceae in the Middle East. The purpose of this study was to evaluate the in vitro activity of fosfomycin against urinary ESBL-producing isolates of Enterobacteriaceae. Between 2004 and 2015, 363 isolates of ESBL-producing Enterobacteriaceae were recovered from high-risk patients suffering from cardiac disorders and were subjected to polymerase chain reaction using specific primers for the blaTEM, blaSHV, and blaCTX-M genes...
October 24, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29064133/-double-carbapenem-and-oral-fosfomycin-for-the-treatment-of-complicated-urinary-tract-infections-caused-by-blandm-harboring-enterobacteriaceae-in-kidney-transplantation
#12
Rossana Rosa, Susan D Rudin, Laura J Rojas, Andrea M Hujer, Armando Perez-Cardona, Federico Perez, Robert A Bonomo, Octavio Martinez, Lilian M Abbo, Jose F Camargo
Infections with carbapenemase-producing carbapenem-resistant Enterobacteriaceae represent an emergent problem worldwide. Treatment of infections caused by New Delhi metallo-beta-lactamase (NDM)-harboring Enterobacteriaceae is particularly challenging as it frequently involves the use of nephrotoxic agents, which is problematic in kidney transplant recipients and non-renal transplants with marginal kidney function. We present two cases of urinary tract infections caused by NDM-harboring Enterobacteriaceae successfully treated with a combination of "double carbapenem" and oral fosfomycin...
October 24, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/29054298/antimicrobial-resistance-in-community-acquired-urinary-tract-infections-in-paris-in-2015
#13
D Chervet, O Lortholary, J-R Zahar, A Dufougeray, B Pilmis, H Partouche
BACKGROUND: Urinary tract infection (UTI) is one of the most frequent community-acquired infection. Escherichia coli resistance has been on the rise since 2000s. METHODS: We conducted a prospective multicenter cohort study including adults who had a positive urine cytobacteriological examination (UCBE) performed in our Parisian suburb laboratory platform from October 2014 to March 2015. RESULTS: A total of 1223 patients were included: 995 (81...
October 17, 2017: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/29048285/high-level-fosfomycin-resistance-in-vancomycin-resistant-enterococcus-faecium
#14
Yan Guo, Adam D Tomich, Christi L McElheny, Vaughn S Cooper, Amelia Tait-Kamradt, Minggui Wang, Fupin Hu, Louis B Rice, Nicolas Sluis-Cremer, Yohei Doi
Of 890 vancomycin-resistant Enterococcus faecium isolates obtained by rectal screening from patients in Pittsburgh, Pennsylvania, USA, 4 had MICs >1,024 μg/mL for fosfomycin. These isolates had a Cys119Asp substitution in the active site of UDP-N-acetylglucosamine enolpyruvyl transferase. This substitution increased the fosfomycin MIC >4-fold and rendered this drug inactive in biochemical assays.
November 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29045415/isolation-identification-and-phenotypic-and-molecular-characterization-of-pathogenic-vibrio-vulnificus-isolated-from-litopenaeus-vannamei
#15
Tao Teng, Liguo Liang, Kai Chen, Bingwen Xi, Jun Xie, Pao Xu
The morphology and the drug sensitivity of the strain GYX2014-1 isolated from the hepatic pancreatic tissue of moribund Litopenaeus vannamei were evaluated by conventional culture characteristics, physical and chemical characteristics, and molecular biology methods. Detection of extracellulase and hemolysin activity shows that the isolated GYX2014-1 has protease, lipase, gelatinase activity, but none of amylase, or lecithinase activity. The 16S rRNA gene (GenBank accession number: KT781675) was analyzed, and a phylogenetic tree analysis showed that the isolated pathogen was most closely related to V...
2017: PloS One
https://www.readbyqxmd.com/read/29038268/urinary-tract-conditions-affect-fosfomycin-activity-against-escherichia-coli-strains-harboring-chromosomal-mutations-involved-in-fosfomycin-uptake
#16
G Martín-Gutiérrez, F Docobo-Pérez, J Rodriguez-Beltrán, J M Rodríguez-Martínez, J Aznar, A Pascual, J Blázquez
The steps by which Escherichia coli strains harboring mutations related with fosfomycin (FOS) resistance arise and spread during urinary tract infections (UTI) is far to be understood. The aim of this study was to evaluate the effect of urine, pH and anaerobiosis on FOS activity against a set of isogenic strains carrying the most prevalent chromosomal mutations conferring FOS-resistance (ΔuhpT, ΔglpT, ΔcyaA and ΔptsI) and their combinations. Also, fosfomycin resistant E. coli clinical isolates from patients with UTI were studied...
October 16, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29037164/local-epidemiology-and-resistance-profiles-in-acute-uncomplicated-cystitis-auc-in-women-a-prospective-cohort-study-in-an-urban-urological-ambulatory-setting
#17
Michael Seitz, Christian Stief, Raphaela Waidelich
BACKGROUND: Acute uncomplicated cystitis (AUC) is a common ailment in the urological setting. Guidelines for urinary tract infections are based on large-scale multi-centre, epidemiological and international studies. The objective of this observational study was to establish whether the results of a multi-centre study on the resistance profile of Escherichia coli (E. coli) in patients with AUC could be directly applied to an urological practice in a major European city or whether there are divergences in the resistance profile...
October 16, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29029067/pharmacodynamics-of-fosfomycin-against-esbl-and-or-carbapenemase-producing-enterobacteriaceae
#18
Fiona Fransen, Kelly Hermans, Maria J B Melchers, Claudia C M Lagarde, Joseph Meletiadis, Johan W Mouton
Background: The increase in antibiotic resistance in Gram-negative bacteria and the limited therapeutic options due to the shortage of new antibiotics have increased the interest of the 'old' antibiotic fosfomycin in the treatment of infections. However, there are contradictory reports on the pharmacodynamics of and emergence of resistance to fosfomycin. Methods: Time-kill assays were performed with 11 ESBL-positive and 3 ESBL-negative strains, exposing the bacteria to 2-fold static concentrations from 0...
October 4, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29020162/successful-therapy-of-a-multi-resistant-ebsl-shv-12-producing-and-fluoroquinolone-resistant-salmonella-enterica-subsp-enterica-serovar-typhi-infection-using-combination-therapy-of-meropenem-and-fosfomycin
#19
Carola-Ellen Kleine, Stefan Schlabe, Gunnar T R Hischebeth, Ernst Molitor, Yvonne Pfeifer, Jan-Christian Wasmuth, Ulrich Spengler
We report a traveler who aquired a Salmonella enterica subsp. enterica serovar Typhi strain with resistance against beta-lactams, cephalosporins (ESBL-SHV-12) and quinolones (PMQR qnrB7). After clinical deterioration using meropenem-monotherapy treatment success was achieved after commencement of fosfomycin in conjunction with high-dose meropenem. The case illustrates clinical challenges of multi-resistant S.Typhi.
July 27, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28993329/inhibition-of-fosfomycin-resistance-protein-fosa-by-phosphonoformate-foscarnet-in-multidrug-resistant-gram-negative-pathogens
#20
Ryota Ito, Adam D Tomich, Christi L McElheny, Roberta Mettus, Nicolas Sluis-Cremer, Yohei Doi
FosA proteins confer fosfomycin resistance in Gram-negative pathogens via glutathione-mediated modification of the antibiotic. In this study, we assessed whether inhibition of FosA by sodium phosphonoformate (PPF; foscarnet), a clinically approved antiviral agent, would reverse fosfomycin resistance in representative Gram-negative pathogens. The inhibitory activity of PPF against purified recombinant FosA from Escherichia coli (FosA3), Klebsiella pneumoniae (FosA(KP)), Enterobacter cloacae (FosA(EC)) and Pseudomonas aeruginosa (FosA(PA)) was determined by steady-state kinetics...
October 9, 2017: Antimicrobial Agents and Chemotherapy
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