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Kevin Kalisz, Ji Buethe, Sachin S Saboo, Suhny Abbara, Sandra Halliburton, Prabhakar Rajiah
Computed tomography is vulnerable to a wide variety of artifacts, including patient- and technique-specific artifacts, some of which are unique to imaging of the heart. Motion is the most common source of artifacts and can be caused by patient, cardiac, or respiratory motion. Cardiac motion artifacts can be reduced by decreasing the heart rate and variability and the duration of data acquisition; adjusting the placement of the data window within a cardiac cycle; performing single-heartbeat scanning; and using multisegment reconstruction, motion-correction algorithms, and electrocardiographic editing...
October 21, 2016: Radiographics: a Review Publication of the Radiological Society of North America, Inc
Essowè Palanga, Denis Filloux, Darren P Martin, Emmanuel Fernandez, Daniel Gargani, Romain Ferdinand, Jean Zabré, Zakaria Bouda, James Bouma Neya, Mahamadou Sawadogo, Oumar Traore, Michel Peterschmitt, Philippe Roumagnac
Cowpea, (Vigna unguiculata L. (Walp)) is an annual tropical grain legume. Often referred to as "poor man's meat", cowpea is one of the most important subsistence legumes cultivated in West Africa due to the high protein content of its seeds. However, African cowpea production can be seriously constrained by viral diseases that reduce yields. While twelve cowpea-infecting viruses have been reported from Africa, only three of these have so-far been reported from Burkina Faso. Here we use a virion-associated nucleic acids (VANA)-based metagenomics method to screen for the presence of cowpea viruses from plants collected from the three agro-climatic zones of Burkina Faso...
2016: PloS One
Jason A Wiesinger, Karen A Cichy, Raymond P Glahn, Michael A Grusak, Mark A Brick, Henry J Thompson, Elad Tako
Dry beans (Phaseolus vulgaris L.) are a nutrient dense food rich in protein and micronutrients. Despite their nutritional benefits, long cooking times limit the consumption of dry beans worldwide, especially in nations where fuelwood for cooking is often expensive or scarce. This study evaluated the nutritive value of 12 dry edible bean lines that vary for cooking time (20 to 89 min) from four market classes (yellow, cranberry, light red kidney, and red mottled) of economic importance in bean consuming regions of Africa and the Americas...
October 18, 2016: Journal of Agricultural and Food Chemistry
Manpreet Randhawa, Steven Wang, James J Leyden, Gabriela O Cula, Alessandra Pagnoni, Michael D Southall
BACKGROUND: Sunscreens are known to protect from sun damage; however, their effects on the reversal of photodamage have been minimally investigated. OBJECTIVE: The aim of the prospective study was to evaluate the efficacy of a facial sun protection factor (SPF) 30 formulation for the improvement of photodamage during a 1-year use. METHODS: Thirty-two subjects applied a broad spectrum photostable sunscreen (SPF 30) for 52 weeks to the entire face...
October 4, 2016: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
Cameron Ratliff, Jordan Gentry, James Kusmierczyk, Kevin M Hartke, Mark J Acierno, Jeffrey M B Musser, Karen E Russell, J Jill Heatley
We collected venous blood samples from 83 apparently healthy Mottled Ducks ( Anas fulvigula ) July 2012-August 2013 on the Texas, USA, Gulf Coast and measured blood gas, electrolyte, biochemical, and hematologic parameters. Age, sex, body condition score, capture year, capture type, and time of day had significant statistical, but not clinically relevant, effects on several analytes. Ducks caught by rocket net had findings consistent with greater stress compared with hand-caught ducks. These analyte data for healthy free-living Mottled Ducks may be useful in the assessment of Mottled Duck population health and in the management and treatment of individual ducks affected by environmental stressors...
October 17, 2016: Journal of Wildlife Diseases
Mina Yasaki, Yasushi Hirano, Hiroyuki Uga, Kaoru Hanada, Tamaki Uehara-Ichiki, Takeshi Toda, Hiromitsu Furuya, Shin-Ichi Fuji
An isometric virus was isolated from a cultivated Adonis plant (A. ramosa). The purified virus particle is 28 nm in diameter and is composed of a single coat protein and a single RNA genome of 3,991 nucleotides. Sequence analysis showed that the virus is closely related to carnation mottle virus. The virus was used to mechanically infect healthy A. ramosa plants, resulting in mosaic and leaf curl symptoms; however, attempts to inoculate carnation plants did not result in infection. We propose the virus as a new carmovirus and have named it adonis mosaic virus (AdMV)...
October 13, 2016: Archives of Virology
Reeta S Mani
The white and fluffy mycelial growth that I had observed 48 hours ago had turned a mottled grey. I inverted the plate of Sabouraud's dextrose agar (SDA) to examine its reverse. A sinister ochre stared at me.
October 2016: Indian Journal of Medical Ethics
Yoshiko Kumagai, Noriko Umegaki-Arao, Takashi Sasaki, Yoshio Nakamura, Hayato Takahashi, Atsuko Ashida, Yuichiro Tsunemi, Makoto Kawashima, Atsushi Shimizu, Akira Ishiko, Koichiro Nakamura, Hayato Tsuchihashi, Masayuki Amagai, Akiharu Kubo
Epidermolysis bullosa simplex (EBS) with migratory circinate erythema (EBS-Migr, MIM 609352) is a rare subtype of EBS, which shows annularly spreading erythema with blister formation and subsequent pigmentation in the inner healed lesions. A heterozygous mutation of c.1649delG in KRT5 has been reported as a specific mutation causing EBS-Migr.(1,2) However, the identical mutation has been reported to cause another rare subtype of EBS, EBS with mottled pigmentation (EBS-MP, MIM 131960).(3-5) Here, we present two familial cases of EBS showing migratory circinate erythema in infancy but mottled pigmentation in the later stages of the disease...
October 12, 2016: Journal of the European Academy of Dermatology and Venereology: JEADV
Silvia L Carvalho, Tatsuya Nagata, Bruna R Junqueira, Larissa G Zanardo, Ana C S Paiva, Claudine M Carvalho
Infectious cDNA clones are an important tool to study the molecular and cellular process of RNA virus infection. In vitro and in vivo transcription systems are the two main strategies used in the generation of infectious cDNA clones for RNA viruses. This study describes the first generation of a full-length infectious cDNA clone of Cowpea mild mottle virus (CPMMV), a Carlavirus. The full-length genome was synthesized by Overlap Extension PCR of two overlapping fragments and cloned in a pUC-based vector under control of the SP6 RNA polymerase promoter...
October 11, 2016: Virus Genes
Lakpa Tamang, Bikramjit Sinha
Physoschistura walongensis, new species, is described from the bank of the Lohit River (upper Brahmaputra River basin) in the eastern most part of the state Arunachal Pradesh at an altitude of 1096 m above sea level. The new species is distinguished from its congeners by the following combination of characters: an incomplete lateral line extending at least to the anal-fin origin and maximally to the tip of the adpressed anal fin, with 71-89 canal pores; a rounded suborbital flap, the posterior margin of which slightly exceeds the anterior orbital margin, occupying less orbital area; body with 11-18 irregular vertical bars on the flank, bars more irregular on the anterior portion of the body than on posterior; 1-4 bars bifurcate ventrally; 1-4 saddles along the ventrolateral side of the body; a moderately thick W-shaped basicaudal bar; a prominent axillary pelvic fin lobe; dark brown mottled markings on the head; and forked caudal fin with 9 upper + 8 lower branched rays...
October 4, 2016: Zootaxa
Jing Lu, Guiying Mai, Yan Luo, Mei Li, Di Cao, Xiao Wang, Hong Yan, SriniVas R Sadda, Lin Lu
PURPOSE: To characterize the appearance of the far peripheral retina of normal eyes using ultra-widefield fluorescein angiography (UWFA). DESIGN: Cross-sectional study. METHODS: This study enrolled 101 eyes with best corrected visual acuity ≥ 20/20, refractive error < 3.00D, and without visible retinal pathologic changes under a slit lamp-based condensing lens. The far peripheral retina was detected by UWFA. Ciliary body thickness (CBT) at 3 mm (CBT1) and 2 mm (CBT2) posterior to the scleral spur was measured by ultrasound biomicroscopy...
September 29, 2016: American Journal of Ophthalmology
Caterina Longo
The aim of the current review is to provide an overview of the use of reflectance confocal microscopy to detect early skin aging signs. This new imaging tool holds the promise to morphologically explore the epidermis and upper dermis at nearly histologic resolution and over time. The main confocal findings of aged skin include the presence of irregular honeycombed pattern, linear skin furrows, mottled pigmentation, and distinct collagen types (coarse and huddled).
October 2016: Dermatologic Clinics
Yan Wei, Beibei Zeng, Hua Zhang, Cheng Chen, Yanli Wu, Nanlan Wang, Yanqiu Wu, Liming Shen
Fluorosis induced by exposure to high level fluoride is quite widespread in the world. The manifestations of fluorosis include dental mottling, bone damage, and impaired malfunction of soft tissues. However, the molecular mechanism of fluorosis has not been clarified until now. To explore the underlying mechanisms of fluorosis and screen out serum biomarkers, we carried out a quantitative proteomics study to identify differentially expressed serum proteins in Wistar rats treated with sodium fluoride (NaF) by using a proteomics approach of isobaric tagging for relative and absolute quantitation (iTRAQ)...
2016: International Journal of Molecular Sciences
Seung Mok Ryu, Jaeyoung Kwon, Young Hye Seo, Eun Gyeong Song, Seong Su Hong, Beom Seok Kim, Jin Sung Hong, Ki Hyun Ryu, Dongho Lee
A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH3OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10μM...
September 26, 2016: Virus Research
Edward J Ciaccio, Govind Bhagat, Suzanne K Lewis, Peter H Green
BACKGROUND: Videocapsule endoscopy is a relative new method to analyze the gastrointestinal tract for the presence of pathologic features. It is of relevance to detect villous atrophy in the small bowel, which is a defining symptom of celiac disease. METHOD: In this tutorial, methods to extract and process videocapsule endoscopy data are elucidated. The algorithms, computer code, and paradigms to analyze image series are described in detail. The topics covered include extraction of data, analysis of texture, eigenanalysis, spectral analysis, three-dimensional projection, and estimation of motility...
September 16, 2016: Computers in Biology and Medicine
Joo Yong Woo, Kwang Ju Jeong, Young Jin Kim, Kyung-Hee Paek
In Arabidopsis, several L-type lectin receptor kinases (LecRKs) have been identified as putative immune receptors. However, to date, there have been few analyses of LecRKs in crop plants. Virus-induced gene silencing of CaLecRK-S.5 verified the role of CaLecRK-S.5 in broad-spectrum resistance. Compared with control plants, CaLecRK-S.5-silenced plants showed reduced hypersensitive response, reactive oxygen species burst, secondary metabolite production, mitogen-activated protein kinase activation, and defense-related gene expression in response to Tobacco mosaic virus pathotype P0 (TMV-P0) infection...
October 2016: Journal of Experimental Botany
K Naimuddin, M Akram, A K Agnihotri
A disease of lentil with symptoms of distortion, mottling and chlorosis in the leaves, shortening of internodes and excessive branching was noticed in lentil at Kanpur, India, during 2012-2014. Results of polymerase chain reaction and reverse transcriptase polymerase chain reaction employed to detect suspected RNA and DNA viruses indicated involvement of a geminivirus, which was further characterized by sequencing of full genome amplified by rolling circle amplification. Analysis of full length DNA-A revealed 96...
2016: Acta Virologica
Lu Devy, Florence Brunet-Possenti
An 88-year-old man with a history of myocardial infarction for which he had required quadruple coronary-artery bypass grafting presented with mottled-appearing purple discoloration of the skin on his toes. One month earlier, he had undergone transapical transcatheter aortic-valve replacement (TAVR)...
September 15, 2016: New England Journal of Medicine
Rita Pinto, Marta Vila-Franca, Cláudia Oliveira Afonso, Conceição Ornelas, Luisa Santos
OBJECTIVE: To report on a case of retinal pigment epithelium (RPE) toxicity apparently associated with ritonavir. METHODS: We describe a case of gradual-onset blurry vision in both eyes in a 30-year-old HIV-positive male on Highly-Active Antiretroviral Therapy (HAART) including ritonavir Results: The patient presented with a visual acuity of 3/10 in each eye, and fundoscopy revealed paracentral pigment mottling. Computerized perimetry showed a ring-scotoma in both eyes...
2013: GMS Ophthalmol Cases
J J Homola, C R Ruetz, S L Kohler, R A Thum
This study used analyses of the genetic structure of a non-game fish species, the mottled sculpin Cottus bairdii to hypothesize probable recolonization routes used by cottids and possibly other Laurentian Great Lakes fishes following glacial recession. Based on samples from 16 small streams in five major Lake Michigan, U.S.A., tributary basins, significant interpopulation differentiation was documented (overall FST  = 0·235). Differentiation was complex, however, with unexpectedly high genetic similarity among basins as well as occasionally strong differentiation within basins, despite relatively close geographic proximity of populations...
September 12, 2016: Journal of Fish Biology
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