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https://www.readbyqxmd.com/read/28071648/foliar-application-of-the-leaf-colonizing-yeast-pseudozyma-churashimaensis-elicits-systemic-defense-of-pepper-against-bacterial-and-viral-pathogens
#1
Gahyung Lee, Sang-Heon Lee, Kyung Mo Kim, Choong-Min Ryu
Yeast associates with many plant parts including the phyllosphere, where it is subject to harsh environmental conditions. Few studies have reported on biological control of foliar pathogens by yeast. Here, we newly isolated leaf-colonizing yeasts from leaves of field-grown pepper plants in a major pepper production area of South Korea. The yeast was isolated using semi-selective medium supplemented with rifampicin to inhibit bacterial growth and its disease control capacity against Xanthomonas axonopodis infection of pepper plants in the greenhouse was evaluated...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28069272/smarcb1-ini1-deficient-sinonasal-carcinoma-shows-methylation-of-rassf1-gene-a-clinicopathological-immunohistochemical-and-molecular-genetic-study-of-a-recently-described-entity
#2
Jan Laco, Marcela Chmelařová, Hana Vošmiková, Kateřina Sieglová, Ivana Bubancová, Pavel Dundr, Kristýna Němejcová, Jaroslav Michálek, Petr Čelakovský, Radovan Mottl, Igor Sirák, Milan Vošmik, Aleš Ryška
The aim of the study was detailed clinicopathological investigation of SMARCB1/INI1-deficient sinonasal carcinomas, including molecular genetic analysis of mutational status and DNA methylation of selected protooncogenes and tumor suppressor genes by means of next generation sequencing (NGS) and methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA). A total of 4/56 (7%) cases of SMARCB1/INI1-deficient carcinomas were detected among 56 sinonasal carcinomas diagnosed over a 19year period using immunohistochemical screening...
October 25, 2016: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28067599/detection-of-cracks-in-dried-spaghetti-using-transmission-images
#3
Takenobu Ogawa, Shuji Adachi
Cracks are formed during the drying process of spaghetti production, which reduces its commercial value and quality; hence, it is very important to detect the cracks during the manufacturing process of dried spaghetti. However, the presence of mottles, originating from wheat bran, hinders automatic identification of cracks. In this study, we developed a simple method to detect the cracks induced in spaghetti using a digital camera. The cracks and mottles were distinguished by pixelating the transmission light image of the spaghetti and analyzing the image based on the geometric characteristics in the histogram consisting of all the pixels...
January 9, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28060137/infants-with-congenital-zika-syndrome-and-ocular-findings-from-s%C3%A3-o-paulo-brazil-spread-of-infection
#4
João Rafael de Oliveira Dias, Camila V Ventura, Paula Delegrego Borba, Bruno de Paula Freitas, Ligia Camera Pierroti, Ana Patricia do Nascimento, Nilva Simeren Bueno de Moraes, Maurício Maia, Rubens Belfort
BACKGROUND/PURPOSE: To describe the ocular findings of two infants with congenital Zika syndrome born in São Paulo state, Brazil, outside the primary outbreak region. METHODS: Two female infants with congenital Zika syndrome were submitted to a complete screening for infectious diseases, as well as ocular examination, fundus photography, and optical coherence tomography of the retina. One of the infants was submitted to ocular ultrasound. RESULTS: Both children showed positive serology for Zika virus, and other infectious diseases were ruled out...
January 2, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28059116/further-characterization-of-maize-chlorotic-mottle-virus-and-its-synergistic-interaction-with-sugarcane-mosaic-virus-in-maize
#5
Qiang Wang, Chao Zhang, Chunyan Wang, Yajuan Qian, Zhenghe Li, Jian Hong, Xueping Zhou
Maize chlorotic mottle virus (MCMV) was first reported in maize in China in 2009. In this study we further analyzed the epidemiology of MCMV and corn lethal necrosis disease (CLND) in China. We determined that CLND observed in China was caused by co-infection of MCMV and sugarcane mosaic virus (SCMV). Phylogenetic analysis using four full-length MCMV cDNA sequences obtained in this study and the available MCMV sequences retrieved from GenBank indicated that Chinese MCMV isolates were derived from the same source...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28055188/assembling-enzymatic-cascade-pathways-inside-virus-based-nanocages-using-dual-tasking-nucleic-acid-tags
#6
Melanie Brasch, Rindia M Putri, Mark Vincent de Ruiter, Daniel Luque, Melissa S T Koay, Jose R Castón, Jeroen J L M Cornelissen
The packaging of proteins into discrete compartments is an essential feature for cellular efficiency. Inspired by Nature, we harness virus-like assemblies as artificial nanocompartments for enzyme-catalyzed cascade reactions. Using the negative charges of nucleic acid tags, we develop a versatile strategy to promote an efficient non-covalent co-encapsulation of enzymes within a single protein cage of Cowpea Chlorotic Mottle Virus (CCMV) at neutral pH. The encapsulation results in stable 21-22 nm-sized CCMV-like particles, which is characteristic of an icosahedral T=1 symmetry...
January 5, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28054163/genetic-variation-and-population-structure-of-cucumber-green-mottle-mosaic-virus
#7
Li-Xia Rao, Yushuang Guo, Li-Li Zhang, Xue-Ping Zhou, Jian Hong, Jian-Xiang Wu
Cucumber green mottle mosaic virus (CGMMV) is a single-stranded, positive sense RNA virus infecting cucurbitaceous plants. In recent years, CGMMV has become an important pathogen of cucurbitaceous crops including watermelon, pumpkin, cucumber and bottle gourd in China, causing serious losses to their production. In this study, we surveyed CGMMV infection in various cucurbitaceous crops grown in Zhejiang Province and in several seed lots purchased from local stores with the dot enzyme-linked immunosorbent assay (dot-ELISA), using a CGMMV specific monoclonal antibody...
January 4, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28031654/aortic-root-rupture-during-transcatheter-aortic-valve-implantation-in-a-patient-with-idiopathic-thrombocytopenic-purpura-utility-of-transesophageal-echocardiography-in-early-detection-and-description-of-a-semiconservative-surgical-management-approach
#8
Jaya Chandrasekhar, Marc Ruel, Donna Nicholson, Marino Labinaz
An 87-year-old man with idiopathic thrombocytopenic purpura and platelet count of 56 × 10(9)/L underwent transesophageal echocardiography (TEE)-guided transcatheter aortic valve implantation using a femoral approach. Post valve deployment, a new pericardial effusion was noted which was successfully drained. Despite this, the patient became hypotensive needing vasopressor support with reaccumulation of pericardial fluid. Emergent sternotomy was performed and a perforation of the right ventricular apex was noted which was sealed with a pledgeted suture...
December 2016: International Journal of Angiology: Official Publication of the International College of Angiology, Inc
https://www.readbyqxmd.com/read/28008222/evaluation-of-peripheral-fundus-autofluorescence-in-eyes-with-wet-age-related-macular-degeneration
#9
Tetsuyuki Suetsugu, Aki Kato, Munenori Yoshida, Tsutomu Yasukawa, Akiko Nishiwaki, Norio Hasegawa, Hideaki Usui, Yuichiro Ogura
PURPOSE: We aimed to evaluate the prevalence of abnormal peripheral fundus autofluorescence (FAF) in wet age-related macular degeneration (AMD) using wide-field imaging instrument. PATIENTS AND METHODS: A retrospective, case-controlled study involving 66 eyes of 46 Japanese wet AMD patients and 32 eyes of 20 control patients was performed. Wide-field FAF images were obtained for typical AMD (37 eyes/28 patients), polypoidal choroidal vasculopathy (PCV) (22 eyes/20 patients), and retinal angiomatous proliferation (RAP) (seven eyes/four patients)...
2016: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28005969/mixed-infections-of-four-viruses-the-incidence-and-phylogenetic-relationships-of-sweet-potato-chlorotic-fleck-virus-betaflexiviridae-isolates-in-wild-species-and-sweetpotatoes-in-uganda-and-evidence-of-distinct-isolates-in-east-africa
#10
Arthur K Tugume, Settumba B Mukasa, Jari P T Valkonen
Viruses infecting wild flora may have a significant negative impact on nearby crops, and vice-versa. Only limited information is available on wild species able to host economically important viruses that infect sweetpotatoes (Ipomoea batatas). In this study, Sweet potato chlorotic fleck virus (SPCFV; Carlavirus, Betaflexiviridae) and Sweet potato chlorotic stunt virus (SPCSV; Crinivirus, Closteroviridae) were surveyed in wild plants of family Convolvulaceae (genera Astripomoea, Ipomoea, Hewittia and Lepistemon) in Uganda...
2016: PloS One
https://www.readbyqxmd.com/read/28003918/superior-mesenteric-artery-syndrome-with-abdominal-compartment-syndrome
#11
Kevin Reece, Rachel Day, Janna Welch
Superior Mesenteric Artery (SMA) syndrome is a condition in which the duodenum becomes compressed between the SMA and the aorta, resulting in bowel obstruction which subsequently compresses surrounding structures. Pressure on the inferior vena cava (IVC) and aorta decreases cardiac output which compromises distal blood flow, resulting in abdominal compartment syndrome with ischemia and renal failure. A 15-year-old male with SMA syndrome presented with 12 hours of pain, a distended, rigid abdomen, mottled skin below the waist, and decreased motor and sensory function in the lower extremities...
2016: Case Reports in Emergency Medicine
https://www.readbyqxmd.com/read/27993590/prevalence-of-anti-retinal-antibodies-in-acute-zonal-occult-outer-retinopathy-azoor-a-comprehensive-review-of-25-cases
#12
Cynthia X Qian, Angeline Wang, David L DeMill, Thiran Jayasundera, Kari Branham, Maria Fernanda Abalem, Naheed Khan, John R Heckenlively
PURPOSE: To perform a comprehensive review and to investigate the presence and role of autoimmune antibodies in 25 cases of acute zonal occult outer retinopathy (AZOOR) identified using the classification originally proposed by J.Donald Gass. DESIGN: Observational case series METHODS: Setting: Institutional Study Population: Twenty-five patients were identified by characteristic symptoms (abrupt onset of photopsias, followed by large scotomata at or connected to the blind spot), ocular findings (paucity of pigmentary changes with no sign of vitreous inflammation and abnormal electroretinogram in at least one eye,) and a negative family history for retinitis pigmentosa...
December 16, 2016: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/27992863/effects-of-high-density-lipoprotein-raising-therapies-on-cardiovascular-outcomes-in-patients-with-type-2-diabetes-mellitus-with-or-without-renal-impairment-the-action-to-control-cardiovascular-risk-in-diabetes-study
#13
Vasilios Papademetriou, Laura Lovato, Costas Tsioufis, William Cushman, William B Applegate, Amy Mottle, Zubin Punthakee, Eric Nylen, Michael Doumas
BACKGROUND: The role of high density lipoprotein-raising interventions in addition to statin therapy in patients with diabetes remains controversial. Chronic kidney disease (CKD) is a strong modifier of cardiovascular (CV) outcomes. We therefore investigated the impact of CKD status at baseline on outcomes in patients with diabetes randomized to standard statin or statin plus fenofibrate treatment in the Action to Control Cardiovascular Risk in Diabetes (ACCORD) lipid trial. METHODS: Among 5,464 participants in the ACCORD lipid trial, 3,554 (65%) were free of CKD at baseline, while 1,910 (35%) had mild to moderate CKD...
December 17, 2016: American Journal of Nephrology
https://www.readbyqxmd.com/read/27965688/mutations-in-rice-yellow-mottle-virus-polyprotein-p2a-involved-in-rymv2-gene-resistance-breakdown
#14
Agnès Pinel-Galzi, Christine Dubreuil-Tranchant, Eugénie Hébrard, Cédric Mariac, Alain Ghesquière, Laurence Albar
Rice yellow mottle virus (RYMV) is one of the major diseases of rice in Africa. The high resistance of the Oryza glaberrima Tog7291 accession involves a null allele of the RYMV2 gene, whose ortholog in Arabidopsis, CPR5, is a transmembrane nucleoporin involved in effector-triggered immunity. To optimize field deployment of the RYMV2 gene and improve its durability, which is often a weak point in varietal resistance, we analyzed its efficiency toward RYMV isolates representing the genetic diversity of the virus and the molecular basis of resistance breakdown...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27960111/p1-independent-replication-and-local-movement-of-rice-yellow-mottle-virus-in-host-and-non-host-plant-species
#15
Grete Nummert, Merike Sõmera, Gabriela Uffert, Erik Abner, Erkki Truve
Sobemovirus P1 protein, characterized previously as a suppressor of posttranscriptional gene silencing, is required for systemic virus spread and infection in plants. Mutations in the ORF1 initiation codon do not affect viral replication indicating P1 is not necessary for this process. Wild type, recombinant and P1 deletion mutants of Cocksfoot mottle virus and Rice yellow mottle virus were used to infect oat, rice, wheat, barley, Arabidopsis thaliana and Nicotiana benthamiana plants. Wild type RYMV, RYMV without P1 and RYMV with CfMV P1 were detected in inoculated leaves of all tested plant species...
December 10, 2016: Virology
https://www.readbyqxmd.com/read/27959752/skin-mottling
#16
Damien Contou, Nicolas de Prost
A 68-year-old woman was admitted to the intensive care unit with respiratory failure and hypotension. Her blood pressure was 70/50 mm Hg, and her heart rate was 125 beats per minute. Physical examination revealed skin mottling on the legs (Panel A). Laboratory values were notable for an..
December 2016: New England Journal of Medicine
https://www.readbyqxmd.com/read/27940096/molecular-dynamics-and-protein-interaction-studies-of-lipopeptide-iturin-a-on-%C3%AE-amylase-of-spodoptera-litura
#17
P Narendra Kumar, T H Swapna, Mohamed Yahya Khan, Jayasimha Rayalu Daddam, Bee Hameeda
The small mottled willow moth (Spodoptera litura) is one of the best-known agricultural pest insects. To understand the insecticidal activity, we have selected iturin A compound produced by Bacillus amyloliquefaciens RHNK22 which showed the strongest and most common inhibitory effect on the Spodoptera litura protein. In this work we have identified the action of iturin A on α- amylase is a major digestive enzyme of Spodoptera litura using docking studies. A 3D model of α- amylase from Spodoptera litura was generated using 2HPH as a template with the help of Modeller7v7...
December 8, 2016: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/27913980/complete-nucleotide-sequences-and-construction-of-full-length-infectious-cdna-clones-of-cucumber-green-mottle-mosaic-virus-cgmmv-in-a-versatile-newly-developed-binary-vector-including-both-35s-and-t7-promoters
#18
Chan-Hwan Park, Hye-Kyoung Ju, Jae-Yeong Han, Jong-Seo Park, Ik-Hyun Kim, Eun-Young Seo, Jung-Kyu Kim, John Hammond, Hyoun-Sub Lim
Seed-transmitted viruses have caused significant damage to watermelon crops in Korea in recent years, with cucumber green mottle mosaic virus (CGMMV) infection widespread as a result of infected seed lots. To determine the likely origin of CGMMV infection, we collected CGMMV isolates from watermelon and melon fields and generated full-length infectious cDNA clones. The full-length cDNAs were cloned into newly constructed binary vector pJY, which includes both the 35S and T7 promoters for versatile usage (agroinfiltration and in vitro RNA transcription) and a modified hepatitis delta virus ribozyme sequence to precisely cleave RNA transcripts at the 3' end of the tobamovirus genome...
December 2, 2016: Virus Genes
https://www.readbyqxmd.com/read/27899288/one-step-reverse-transcription-loop-mediated-isothermal-amplification-for-the-detection-of-maize-chlorotic-mottle-virus-in-maize
#19
Ling Chen, Zhiyuan Jiao, Dongmei Liu, Xingliang Liu, Zihao Xia, Congliang Deng, Tao Zhou, Zaifeng Fan
Maize chlorotic mottle virus (MCMV) is spreading in many regions worldwide, causing maize lethal necrosis when co-infected with a potyvirid. In this study, one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to detect MCMV in maize. A set of four specific primers was designed based on the conserved coat protein gene sequences of MCMV. The RT-LAMP could be completed within 60min under isothermal condition at 63°C. The sensitivity test showed that the RT-LAMP was about 10-fold more sensitive than RT-PCR and no cross-reactivity was detected with other viral pathogens infecting maize in China...
February 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/27881977/different-virus-derived-sirnas-profiles-between-leaves-and-fruits-in-cucumber-green-mottle-mosaic-virus-infected-lagenaria-siceraria-plants
#20
Junmin Li, Hongying Zheng, Chenhua Zhang, Kelei Han, Shu Wang, Jiejun Peng, Yuwen Lu, Jinping Zhao, Pei Xu, Xiaohua Wu, Guojing Li, Jianping Chen, Fei Yan
RNA silencing is an evolutionarily conserved antiviral mechanism, through which virus-derived small interfering RNAs (vsiRNAs) playing roles in host antiviral defense are produced in virus-infected plant. Deep sequencing technology has revolutionized the study on the interaction between virus and plant host through the analysis of vsiRNAs profile. However, comparison of vsiRNA profiles in different tissues from a same host plant has been rarely reported. In this study, the profiles of vsiRNAs from leaves and fruits of Lagenaria siceraria plants infected with Cucumber green mottle mosaic virus (CGMMV) were comprehensively characterized and compared...
2016: Frontiers in Microbiology
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