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https://www.readbyqxmd.com/read/28336589/first-complete-genome-sequence-of-cucumber-green-mottle-mosaic-virus-isolated-from-australia
#1
Monica A Kehoe, Roger A C Jones, Brenda A Coutts
We present here the first complete genome sequence of the tobamovirus Cucumber green mottle mosaic virus (CGMMV) from Australia, obtained from an infected cucumber plant. Compared with other CGMMV genomes, its closest nucleotide identities were 99.6% to KP772568, 99.3% to KF155229, and 99.1% to DQ767631 from Canada, Israel, and India, respectively.
March 23, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28329614/dyschromatosis-universalis-hereditaria-report-of-six-cases-from-a-family
#2
Swapan Kumar Sardar, Anupam Das, Debabrata Bandyopadhyay Bandyopadhyay
Dyschromatosis universalis hereditaria (DUH) is a rare pigmentary disorder characterized by the presence of mottled hyperpigmented and hypopigmented macules over the trunk, extremities, and face. We have presented a case series comprised of six members of a family who had numerous hyperpigmented and hypopigmented macules distributed all over the body. Histological findings were suggestive of dyschromatosis universalis hereditaria.
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28320287/subcellular-localization-and-detection-of-tobacco-mosaic-virus-orf6-protein-by-immunoelectron-microscopy
#3
T N Erokhina, E A Lazareva, K R Richert-Pöggeler, E V Sheval, A G Solovyev, S Y Morozov
Members of the genus Tobamovirus represent one of the best-characterized groups of plant positive, single stranded RNA viruses. Previous studies have shown that genomes of some tobamoviruses contain not only genes coding for coat protein, movement protein, and the cistron coding for different domains of RNA-polymerase, but also a gene, named ORF6, coding for a poorly conserved small protein. The amino acid sequences of ORF6 proteins encoded by different tobamoviruses are highly divergent. The potential role of ORF6 proteins in replication of tobamoviruses still needs to be elucidated...
January 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28318359/zika-virus-and-eye
#4
Rupesh Agrawal, Hnin Hnin Oo, Praveen Kumar Balne, Lisa Ng, Louis Tong, Yee Sin Leo
Zika virus (ZIKV), a mosquito-borne flavivirus, is the latest global health concern. Transmission is mainly via Aedes mosquitoes and the infection can be diagnosed on molecular or serologic testings. It typically causes a mild self-remitting illness of low-grade fever, maculopapular rash, and myalgia, but when severe, it is associated with neurological deficits and congenital structural defects. Ocular manifestations are usually mild like nonpurulent conjunctivitis in adults, though it may be linked to uveitis, maculopathy, and hypertensive iridocyclitis...
March 20, 2017: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/28306558/monitoring-of-the-physical-exam-in-sepsis
#5
Radu Postelnicu, Laura Evans
PURPOSE OF REVIEW: Monitoring of mental status and peripheral circulatory changes can be accomplished noninvasively in patients in the ICU. Emphasis on physical examination in conditions such as sepsis have gained increased attention as these evaluations can often serve as a surrogate marker for short-term treatment efficacy of therapeutic interventions. Sepsis associated encephalopathy and mental status changes correlate with worse prognosis in patients. Evaluation of peripheral circulation has been shown to be a convenient, easily accessible, and accurate marker for prognosis in patients with septic shock...
March 16, 2017: Current Opinion in Critical Care
https://www.readbyqxmd.com/read/28297032/ultra-wide-field-fundus-autofluorescence-and-spectral-domain-optical-coherence-tomography-findings-in-syphilitic-outer-retinitis
#6
Mohamed G A Saleh, John Peter Campbell, Paul Yang, Phoebe Lin
BACKGROUND AND OBJECTIVE: To determine the ultra-wide-field fundus autofluorescence (UWFFAF) and optical coherence tomography (OCT) features of syphilitic outer retinopathy (SOR). PATIENTS AND METHODS: Retrospective chart review. RESULTS: Three patients with SOR were investigated. Treatment with parenteral penicillin led to improvement of outer retinopathy, visual acuity, and symptoms. UWFFAF showed speckled hyperautofluorescence, hypoautofluorescence, and normal autofluorescence, similar to what has been described as a trizonal pattern in acute zonal occult outer retinopathy (AZOOR) in the chronic case of SOR, but with hyperautofluorescent areas in the two acute cases...
March 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28270819/effects-of-low-ph-on-photosynthesis-related-physiological-parameters-and-nutrient-profiles-of-citrus
#7
An Long, Jiang Zhang, Lin-Tong Yang, Xin Ye, Ning-Wei Lai, Ling-Ling Tan, Dan Lin, Li-Song Chen
Seedlings of "Xuegan" (Citrus sinensis) and "Sour pummelo" (Citrus grandis) were irrigated daily with a nutrient solution at a pH of 2.5, 3, 4, 5, or 6 for 9 months. Thereafter, the following responses were investigated: seedling growth; root, stem, and leaf concentrations of nutrient elements; leaf gas exchange, pigment concentration, ribulose-1,5-bisphosphate carboxylase/oxygenase activity and chlorophyll a fluorescence; relative water content, total soluble protein level, H2O2 production and electrolyte leakage in roots and leaves...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28266088/fluoride-or-and-aluminum-induced-toxicity-in-guinea-pig-teeth-with-the-low-expression-of-dentine-phosphoprotein
#8
Tianlong Han, Min Wang, Chunfang Cao, Huacheng Chen, Guanghe Zhang, Liping Wang, Jundong Wang
This study investigated the damage and expression of dentine phosphoprotein (DPP) in guinea pig teeth by the administration of fluoride (F) or/and aluminum (Al). Fifty-two guinea pigs were divided randomly into four groups (control, F, Al, and F+Al). F (150 mg NaF/L) or/and Al (300 mg AlCl3 /L) were added in their drinking water for 90 days. The levels of F ion, dentine sialophosphoprotein (DSPP) gene, and DPP protein in incisor and molar were determined, respectively. The results showed that the concentrations of F ion in F and F+Al groups were increased significantly...
March 7, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28263842/a-cgmmv-genome-replicon-vector-with-partial-sequences-of-coat-protein-gene-efficiently-expresses-gfp-in-nicotiana-benthamiana
#9
A Abdul Kader Jailani, Vikas Solanki, Anirban Roy, T Sivasudha, Bikash Mandal
A highly infectious clone of Cucumber green mottle mosaic virus (CGMMV), a cucurbit-infecting tobamovirus was utilized for designing of gene expression vectors. Two versions of vector were examined for their efficacy in expressing the green fluorescent protein (GFP) in Nicotiana benthamiana. When the GFP gene was inserted at the stop codon of coat protein (CP) gene of the CGMMV genome without any read-through codon, systemic expression of GFP, as well as virion formation and systemic symptoms expression were obtained in N...
March 2, 2017: Virus Research
https://www.readbyqxmd.com/read/28262381/pediatric-sepsis-secondary-to-an-occult-dental-abscess-a%C3%A2-case-report
#10
Peter Holmberg, Thomas Hellmich, James Homme
BACKGROUND: In general, hematogenous spread of bacteria in children is uncommon. Bacteremia, however, is a known complication of dental procedures and severe caries, but is infrequently associated with primary, asymptomatic, non-procedural-related, dentoalveolar infection. CASE REPORT: The patient is a 7-year-old previously healthy boy who presented to the Emergency Department (ED) with "fever, mottling, and shaking chills." In the ED, he appeared systemically ill with fever, mottling, delayed capillary refill, and rigors...
March 2, 2017: Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28260140/complete-nucleotide-sequences-of-m-and-l-rnas-from-a-new-pepper-infecting-tospovirus-pepper-chlorotic-spot-virus
#11
Kuo-Shiou Huang, Chia-Hsing Tai, Ying-Huey Cheng, Shu-Hui Lin, Tsung-Chi Chen, Fuh-Jyh Jan
Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral (v) strand and the glycoprotein precursor (Gn/Gc) of 1122 aa (127.6 kDa) in the viral complementary (vc) strand. The L RNA has 8859 nts, encoding the RNA-dependent RNA polymerase (RdRp) of 2873 aa (330...
March 4, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28259077/assessment-of-the-efficacy-of-membrane-filtration-processes-to-remove-human-enteric-viruses-and-the-suitability-of-bacteriophages-and-a-plant-virus-as-surrogates-for-those-viruses
#12
N Shirasaki, T Matsushita, Y Matsui, K Murai
Here, we evaluated the efficacy of direct microfiltration (MF) and ultrafiltration (UF) to remove three representative human enteric viruses (i.e., adenovirus [AdV] type 40, coxsackievirus [CV] B5, and hepatitis A virus [HAV] IB), and one surrogate of human caliciviruses (i.e., murine norovirus [MNV] type 1). Eight different MF membranes and three different UF membranes were used. We also examined the ability of coagulation pretreatment with high-basicity polyaluminum chloride (PACl) to enhance virus removal by MF...
February 24, 2017: Water Research
https://www.readbyqxmd.com/read/28253931/zika-virus-infection-of-cellular-components-of-the-blood-retinal-barriers-implications-for-viral-associated-congenital-ocular-disease
#13
Tracoyia Roach, Donald J Alcendor
BACKGROUND: Ocular abnormalities present in microcephalic infants with presumed Zika virus (ZIKV) congenital disease includes focal pigment mottling of the retina, chorioretinal atrophy, optic nerve abnormalities, and lens dislocation. Target cells in the ocular compartment for ZIKV infectivity are unknown. The cellular response of ocular cells to ZIKV infection has not been described. Mechanisms for viral dissemination in the ocular compartment of ZIKV-infected infants and adults have not been reported...
March 3, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28245443/fundus-autofluorescence-imaging-in-the-assessment-of-acute-zonal-occult-outer-retinopathy
#14
Qian Wang, Libin Jiang, Weiyu Yan, Wenbin Wei, Timothy Y Y Lai
PURPOSE: To evaluate the fundus autofluorescence (FAF) findings in eyes with acute zonal occult outer retinopathy (AZOOR) and the relationships between the FAF, spectral-domain optical coherence tomography (SD-OCT), and Humphrey visual field (HVF) findings. METHODS: We performed a retrospective review of the clinical features and FAF, SD-OCT, and HVF findings of 23 eyes of 22 consecutive patients with AZOOR. The mean deviation (MD) values of HVF were compared between areas of normal and abnormal FAF...
March 1, 2017: Ophthalmologica. Journal International D'ophtalmologie
https://www.readbyqxmd.com/read/28245334/association-of-type-1-diabetes-vs-type-2-diabetes-diagnosed-during-childhood-and-adolescence-with-complications-during-teenage-years-and-young-adulthood
#15
Dana Dabelea, Jeanette M Stafford, Elizabeth J Mayer-Davis, Ralph D'Agostino, Lawrence Dolan, Giuseppina Imperatore, Barbara Linder, Jean M Lawrence, Santica M Marcovina, Amy K Mottl, Mary Helen Black, Rodica Pop-Busui, Sharon Saydah, Richard F Hamman, Catherine Pihoker
Importance: The burden and determinants of complications and comorbidities in contemporary youth-onset diabetes are unknown. Objective: To determine the prevalence of and risk factors for complications related to type 1 diabetes vs type 2 diabetes among teenagers and young adults who had been diagnosed with diabetes during childhood and adolescence. Design, Setting, and Participants: Observational study from 2002 to 2015 in 5 US locations, including 2018 participants with type 1 and type 2 diabetes diagnosed at younger than 20 years, with single outcome measures between 2011 and 2015...
February 28, 2017: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/28243802/desmodium-mottle-virus-the-first-legumovirus-genus-begomovirus-from-east-africa
#16
Happyness G Mollel, Peter Sseruwagi, Joseph Ndunguru, Titus Alicai, John Colvin, Jesús Navas-Castillo, Elvira Fiallo-Olivé
A novel bipartite legumovirus (genus Begomovirus, family Geminiviridae), that naturally infects the wild leguminous plant Desmodium sp. in Uganda, was molecularly characterized and named Desmodium mottle virus. The highest nucleotide identities for DNA-A, obtained from two field-collected samples, were 79.9% and 80.1% with the legumovirus, soybean mild mottle virus. DNA-B had the highest nucleotide identities (65.4% and 66.4%) with a typical non-legumovirus Old World begomovirus, African cassava mosaic virus...
February 27, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28239662/zika-virus-infects-cells-lining-the-blood-retinal-barrier-and-causes-chorioretinal-atrophy-in-mouse-eyes
#17
Pawan Kumar Singh, John-Michael Guest, Mamta Kanwar, Joseph Boss, Nan Gao, Mark S Juzych, Gary W Abrams, Fu-Shin Yu, Ashok Kumar
Zika virus (ZIKV) is an important pathogen that causes not only neurologic, but also ocular, abnormalities. Thus, it is imperative that models to study ZIKV pathogenesis in the eye are developed to identify potential targets for interventions. Here, we studied ZIKV interactions with human retinal cells and evaluated ZIKV's pathobiology in mouse eyes. We showed that cells lining the blood-retinal barrier (BRB), the retinal endothelium, and retinal pigment epithelium (RPE) were highly permissive and susceptible to ZIKV-induced cell death...
February 23, 2017: JCI Insight
https://www.readbyqxmd.com/read/28224251/complete-genome-sequence-of-sunflower-ring-blotch-virus-a-new-potyvirus-infecting-sunflower-in-argentina
#18
Dariel Cabrera Mederos, Nicolás Bejerman, Verónica Trucco, Soledad de Breuil, Sergio Lenardon, Fabián Giolitti
The complete genome sequence of sunflower ring blotch virus (SuRBV), a previously undescribed potyvirus infecting sunflower in Argentina, is reported. The SuRBV genome comprises 9555 nucleotides (nt) and encodes a polyprotein of 3061 amino acids, flanked by 5' and 3' untranslated regions of 117 and 255 nt, respectively. Phylogenetic analysis showed that SuRBV belongs to the potato virus Y (PVY) subgroup and clusters together with sunflower chlorotic mottle virus and bidens mosaic virus. Percentage nucleotide identity between the whole genomes of SuRBV and BiMV was 70...
February 21, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28220324/virus-surveys-of-capsicum-spp-in-the-republic-of-benin-reveal-the-prevalence-of-pepper-vein-yellows-virus-and-the-identification-of-a-previously-uncharacterised-polerovirus-species
#19
Leonard Afouda, Daouda Kone, Valerien Zinsou, Laurence Dossou, Lawrence Kenyon, Stephan Winter, Dennis Knierim
Surveys were conducted in 2014 and 2015 in Southern and Northern Benin, respectively, to identify the viruses infecting peppers (Capsicum spp.). The samples were screened by ELISA for cucumber mosaic virus (CMV), pepper veinal mottle virus (PVMV), potato virus Y (PVY) and tomato yellow leaf curl virus (TYLCV). A generic reverse transcription PCR (RT-PCR) was used to test for the presence of poleroviruses. ELISA tests confirmed the prevalence of all viruses, while the RT-PCR detected pepper vein yellows virus (PeVYV) which is reported for the first time in Benin...
February 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28217131/incomplete-infection-of-secondarily-infected-potato-plants-an-environment-dependent-underestimated-mechanism-in-plant-virology
#20
Lukas Bertschinger, Lukas Bühler, Brice Dupuis, Brion Duffy, Cesare Gessler, Gregory A Forbes, Ernst R Keller, Urs C Scheidegger, Paul C Struik
The common assumption in potato virus epidemiology is that all daughter tubers produced by plants coming from infected mother tubers (secondary infection) will become infected via systemic translocation of the virus during growth. We hypothesize that depending on the prevalent environmental conditions, only a portion of the daughter tubers of a plant that is secondarily infected by viruses may become infected. To test this hypothesis experimental data from standardized field experiments were produced in three contrasting environments at 112, 3280, and 4000 m a...
2017: Frontiers in Plant Science
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