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https://www.readbyqxmd.com/read/28213069/a-pilot-study-of-cell-mediated-gene-therapy-for-spinal-cord-injury-in-mini-pigs
#1
Islamov Rustem Robertovich, Sokolov Mikhail Evgenyevich, Bashirov Farid Vagizovich, Fadeev Filip Olegovich, Shmarov Maxim Michaylovich, Naroditskiy Boris Savelyevich, Povysheva Tatyana Vyacheslavovna, Shaymardanova Gulnara Ferdinantovna, Yakupov Radik Albertovich, Chelyshev Yuri Aleksandrovich, Lavrov Igor Aleksandrovich
Currently, in clinical practice there is no efficient way to overcome the sequences of neurodegeneration after spinal cord traumatic injury. Using a new experimental model of spinal cord contusion injury on miniature pigs, we proposed to deliver therapeutic genes encoding vascular endothelial growth factor (VEGF), glial cell line-derived neurotrophic factor (GDNF) and neural cell adhesion molecule (NCAM) to the damaged area, using umbilical cord blood mononuclear cells (UCBC). In this study, genetically engineered UCBC (2×10(6) cells in 200 ml of saline) were injected intrathecally to mini-pigs 10days after SCI...
February 14, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28210466/antimicrobial-effects-of-ferula-gummosa-boiss-gum-against-extended-spectrum-%C3%AE-lactamase-producing-acinetobacter-clinical-isolates
#2
Fatemeh Farid Afshar, Parvaneh Saffarian, Hamideh Mahmoodzadeh Hosseini, Fereshteh Sattarian, Mohsen Amin, Abbas Ali Imani Fooladi
BACKGROUND AND OBJECTIVES: Acinetobacter spp. are important causes of nosocomial infections. They possess various antibiotic resistance mechanisms including extended spectrum beta lactamases (ESBLs). The aim of this study was to determine antibiotic resistance profile of Acinetobacter clinical isolates especially among ESBL-producing strains and to investigate the antimicrobial effects of oleo-gum-resin extract and essential oil of Ferula gummosa Boiss. MATERIALS AND METHODS: 120 Acinetobacter strains were isolated from various clinical samples of hospitalized patients in Baqiyatallah hospital, Tehran during 2011-2012...
August 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28210462/accuracy-of-a-new-rapid-antigen-detection-test-for-pulmonary-tuberculosis
#3
Rasoul Aliannejad, Ahmadreza Bahrmand, Hamidreza Abtahi, Mahnaz Seifi, Enayat Safavi, Farid Abdolrahimi, Shahriyar Shahriaran
BACKGROUND AND OBJECTIVES: Tuberculosis (TB) is a major problem in the world. Treatment and control of TB needs detection of the Mycobacterium tuberculosis (MT) in the proper samples. While smear doesn't have enough sensitivity, culture and PCR are expensive, time consuming and unavailable in many centers. Recent development of a rapid TB antigen detection test (PrTBK) at Pasteur Institute of Iran could give a simple way for diagnosis of TB in about two hours. In this test the antigen-antibody complex will change color when gold conjugated mouse anti-rabbit antibody detects specific MT cell wall antigen in suspected samples...
August 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28203336/a-5-fr-externalized-nephroureteral-catheter-as-the-sole-protective-device-for-pediatric-pyeloplasty-the-experiences-of-142-patients
#4
Mansour Mollaeian, Maryam Ghavami-Adel, Farid Eskandari, Arash Mollaeian
BACKGROUND: Pyeloplasty for ureteropelvic junction obstruction correction is a common procedure, but the optimal method for protective diversion after pyeloplasty is still a matter of debate. OBJECTIVES: Here, we present our clinical trial experience using a single percutaneous externalized nephroureteral (NU) 5-Fr catheter (infant feeding tube) with multiple side holes as the sole instrument of drainage to provide a protective mechanism. MATERIALS AND METHODS: In this prospective study, we analyzed the charts of 142 patients who underwent pyeloplasty from August 2001 through October 2008...
October 2016: Iranian Journal of Pediatrics
https://www.readbyqxmd.com/read/28197400/effect-of-gingival-fibroblasts-and-ultrasound-on-dogs-root-resorption-during-orthodontic-treatment
#5
Jacqueline Crossman, Ali H Hassan, Ali Saleem, Nayef Felemban, Saleh Aldaghreer, Elham Fawzi, Mamdouh Farid, Khaled Abdel-Ghaffar, Ausama Gargoum, Tarek El-Bialy
OBJECTIVES: To investigate the effect of using osteogenic induced gingival fibroblasts (OIGFs) and low intensity pulsed ultrasound (LIPUS) on root resorption lacunae volume and cementum thickness in beagle dogs that received orthodontic tooth movement. MATERIALS AND METHODS: Seven beagle dogs were used, from which gingival cells (GCs) were obtained and were induced osteogenically to produce OIGFs. Each third and fourth premolar was randomly assigned to one of the five groups, namely, LIPUS, OIGFs, bone morphogenetic protein-2 (BMP-2), OIGFs + LIPUS, and control...
January 2017: Journal of Orthodontic Science
https://www.readbyqxmd.com/read/28195547/phenolic-compounds-from-citrus-leaves-antioxidant-activity-and-enzymatic-browning-inhibition
#6
Bachra Khettal, Nabil Kadri, Karim Tighilet, Ahmed Adjebli, Farid Dahmoune, Fadila Maiza-Benabdeslam
Background Phenolic compounds from Citrus are known to be a topic of many studies due to their biological properties including antioxidant activity. Methods Methanolic and aqueous extracts were isolated from Citrus leaves of different species (C. clementina, C. limon, C. hamlin, C. navel, C. aurantifolia, C. aurantium and C. grandis) harvested in Algeria. Results The results showed that aqueous extracts of all species are rich in total phenolic compounds and flavonoids (from 68.23 to 125.28 mg GAE/g DM) and (from 11...
February 14, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28193537/preparation-characterization-and-in-vivo-evaluation-of-a-combination-delivery-system-based-on-hyaluronic-acid-jeffamine-hydrogel-loaded-with-phbv-plga-blend-nanoparticles-for-prolonged-delivery-of-teriparatide
#7
Nika Bahari Javan, Hamed Montazeri, Leila Rezaie Shirmard, Nersi Jafary Omid, Ghullam Reza Barbari, Mohsen Amini, Mohammad Hossein Ghahremani, Morteza Rafiee-Tehrani, Farid Abedin Dorkoosh
In the current study, biodegradable PHBV/PLGA blend nanoparticles (NPs) containing Teriparatide were loaded in hyaluronic acid/jeffamine (HA-JEF ED-600) hydrogel to prepare a combination delivery system (CDS) for prolonged delivery of Teriparatide. The principal purpose of the present study was to formulate an effective and prolonged Teriparatide delivery system in order to reduce the frequency of injection and thus enhance patient's compliance. Morphological properties, swelling behaviour, crosslinking efficiency and rheological characterization of HA-JEF ED-600 hydrogel were evaluated...
February 10, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28183602/a-biological-method-for-in-situ-synthesis-of-hydroxyapatite-coated-magnetite-nanoparticles-using-enterobacter-aerogenes-characterization-and-acute-toxicity-assessments
#8
Elham Ahmadzadeh, Farid Talebnia Rowshan, Morteza Hosseini
Hydroxyapatite (HA)-coated magnetite nanoparticles (MNPs) are being widely investigated for various applications in medical engineering and wastewater treatment. In this work, the MNPs were thoroughly coated by bacterial synthesized HA nanoparticles during biomineralization process using Enterobacter aerogenes. The resulting bacterial-induced precipitate was then calcined at 600°C and investigated with respect to structural characteristics, particle size and magnetic strength by XRD, FT-IR, SEM, EDS, TEM and VSM analyses...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28182044/efficacy-and-safety-of-early-tacrolimus-conversion-to-sirolimus-after-kidney-transplantation-long-term-results-of-a-prospective-randomized-study
#9
A E El-Agroudy, S M Alarrayed, S M Al-Ghareeb, E Farid, H Alhelow, S Abdulla
We report a prospective, open-label, randomized study to evaluate the safety and efficacy of converting patients with a stable renal function from tacrolimus (Tac)-based regimen to a sirolimus (SRL)-based regimen after kidney transplantation. Fifty-eight low-risk renal allograft recipients who receiving Tac 6 months posttransplant, were randomly assigned to continue Tac (n = 29) or convert to SRL (n = 29). We evaluated the 3-year outcomes including patient and graft survival, graft function, and safety profile...
January 2017: Indian Journal of Nephrology
https://www.readbyqxmd.com/read/28181985/artiss-sealant%C3%A2-an-alternative-to-stapling-skin-grafts-on-the-dorsal-side-of-the-hand-and-fingers
#10
David Boccara, Antoine De Runz, Farid Bakara, Marc Chaouat, Maurice Mimoun
The aim of this study was to evaluate the benefit of using a new slow-setting fibrin sealant compared with staples for adherence of split-thickness skin grafts on the backs of hands and fingers. Staple removal is often painful for patients. Twenty-eight patients were included for a graft total of 34 hands. The authors chose to test this sealant on zones that are often difficult to graft given their varied topographies. Its low concentration of thrombin (4 IU/ml) compared with other sealants provides it with prolonged polymerization time (60 seconds) making it easier to use in this location...
February 7, 2017: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/28181107/stability-of-pacing-indices-and-need-for-pacing-in-cardiac-transplant-patients-over-1%C3%A2-year-of-follow-up
#11
Talha A Farid, Mohamed A Omer, Kensey Gosch, Ashley Moser, Bethany Austin, Anthony Magalski, Alan P Wimmer
BACKGROUND: A significant minority of cardiac transplant patients require permanent pacemaker (PPM) implant, primarily for sinus node dysfunction. The stability of pacing indices has not been determined in this unique patient population, and data regarding ongoing need for pacing are limited. METHODS: Pacing indices (sensing, threshold, and impedance) as well as the percentage of time patients required pacing were recorded, from 30 cardiac transplant patients that underwent PPM implant, over 1 year of follow-up...
February 8, 2017: Journal of Interventional Cardiac Electrophysiology: An International Journal of Arrhythmias and Pacing
https://www.readbyqxmd.com/read/28180013/the-influence-of-internal-wall-and-floor-covering-materials-and-ventilation-type-on-indoor-radon-and-thoron-levels-in-hospitals-of-kermanshah-iran
#12
Meghdad Pirsaheb, Farid Najafi, Abbas Haghparast, Lida Hemati, Kiomars Sharafi, Nematullah Kurd
BACKGROUND: Building materials and the ventilation rate of a building are two main factors influencing indoor radon and thoron levels (two radioactive gases which have the most important role in human natural radiation exposure within dwellings). OBJECTIVES: This analytical descriptive study was intended to determine the relationship between indoor radon and thoron concentrations and the building materials used in interior surfaces, as well as between those concentrations and the type of ventilation system (natural or artificial)...
October 2016: Iranian Red Crescent Medical Journal
https://www.readbyqxmd.com/read/28179273/radiation-free-alternative-donor-hct-for-fanconi-anemia-patients-results-from-a-prospective-multi-institutional-study
#13
Parinda A Mehta, Stella M Davies, Thomas Leemhuis, Kasiani Myers, Nancy A Kernan, Susan E Prockop, Andromachi Scaradavou, Richard J O'Reilly, David A Williams, Leslie Lehmann, Eva Guinan, David Margolis, K Scott Baker, Adam Lane, Farid Boulad
Fanconi anemia (FA) is an inherited bone marrow failure syndrome characterized by chromosomal fragility, progressive marrow failure and cancer predisposition. Hematopoietic cell transplantation (HCT) is curative for FA-related marrow failure or leukemia, but both radiation exposure during transplant and graft-versus host disease (GVHD) may increase risk of later malignancies of the head and neck and anogenital area. In this study we tested a radiation-free conditioning regimen with a T-cell depleted graft to eliminate radiation exposure and minimize early as well as late toxicities of transplant...
February 8, 2017: Blood
https://www.readbyqxmd.com/read/28177788/a-fast-and-accurate-method-of-detecting-aleutian-mink-disease-virus-in-blood-and-tissues-of-chronically-infected-mink
#14
A H Farid, Priyanka P Rupasinghe
The objective of this study was to assess sensitivity of the Omni Klentaq-LA DNA polymerase for detecting Aleutian mink disease virus (AMDV) in mink blood and tissues by PCR without DNA extraction. The presence of AMDV DNA was directly tested by Klentaq in plasma, serum, whole blood and spleen homogenates of 188 mink 4 and 16 months after inoculation with the virus. Samples from bone marrow, small intestine, liver, lungs, kidneys and lymph nodes of 20 of the same mink were also tested by Klentaq. DNA was extracted from paired samples of plasma and the aforesaid tissues by a commercial nucleic acid extraction kit (Dynabeads Silane) and tested by PCR...
January 18, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28174601/development-of-a-genetically-programed-vanillin-sensing-bacterium-for-high-throughput-screening-of-lignin-degrading-enzyme-libraries
#15
Barindra Sana, Kuan Hui Burton Chia, Sarada S Raghavan, Balamurugan Ramalingam, Niranjan Nagarajan, Jayasree Seayad, Farid J Ghadessy
BACKGROUND: Lignin is a potential biorefinery feedstock for the production of value-added chemicals including vanillin. A huge amount of lignin is produced as a by-product of the paper industry, while cellulosic components of plant biomass are utilized for the production of paper pulp. In spite of vast potential, lignin remains the least exploited component of plant biomass due to its extremely complex and heterogenous structure. Several enzymes have been reported to have lignin-degrading properties and could be potentially used in lignin biorefining if their catalytic properties could be improved by enzyme engineering...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28164561/frequency-of-foxp3-regulatory-t-cells-in-the-blood-of-chronic-hepatitis-c-patients-with-immune-mediated-skin-manifestations-relationship-to-hepatic-condition-and-viral-load
#16
Carmen Farid, Wafaa El Sheikh, Rania Swelem, Engy El-Ghitany
BACKGROUND: Chronic hepatitis C (CHC) infection causes a wide range of immune mediated hepatic and extrahepatic manifestations. Cutaneous manifestations constitute a major portion of the latter group. Host immune response to the virus - particularly regulatory T lymphocytes - is important in determining the outcome of the infection and the possibility of extrahepatic manifestations. The present study aims to investigate the relationship between immune-mediated cutaneous manifestations of CHC infection and the frequency of CD4+CD25 high FOXP3+ T regulatory lymphocytes...
December 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28160428/synthesis-optimization-shaping-and-heat-reallocation-evaluation-of-the-hydrophilic-metal-organic-framework-mil-160-al
#17
Anastasia Permyakova, Oleksandr Skrylnyk, Emilie Courbon, Maame Affram, Sujing Wang, U-Hwang Lee, Anil H Valekar, Farid Nouar, Georges Mouchaham, Thomas Devic, Guy De Weireld, Jong-San Chang, Nathalie Steunou, Marc Frère, Christian Serre
The energy storage capacities of a series of water-stable porous MOFs, based on high valence metal cations (Al3+, Fe3+, Cr3+, Ti4+, Zr4+) and polycarboxylate linkers, have been evaluated in the typical conditions of seasonal energy storage devices. It turned out that the microporous hydrophilic Al-dicarboxylate MIL-160(Al) exhibits one of the best performance. To assess the properties of this material for space heating applications and on a laboratory pilot scale with an open reactor, a novel synthetic route involving safer, greener conditions was developed...
February 3, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28157445/creatinine-based-non-phospholipid-vesicular-carrier-for-improved-oral-bioavailability-of-azithromycin
#18
Shafi Ullah, Muhammad Raza Shah, Mohammad Shoaib, Muhammad Imran, Syed Wadood Ali Shah, Imdad Ali, Farid Ahmed
CONTEXT: Novel, safe, efficient and cost effective nano-carriers from renewable resources have got greater interest for enhancing solubility and bioavailability of hydrophobic dugs. OBJECTIVES: This study reports the synthesis of a novel biocompatible non-phospholipid human metabolite "Creatinine" based niosomal delivery system for Azithromycin improved oral bioavailability. METHODS: Synthesized surfactant was characterized through spectroscopic and spectrometric techniques and then evaluated for niosomal vesicles formation potential using Azithromycin as model drug...
February 3, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28156177/abnormalities-in-hippocampal-volume-of-glioma-patients-prior-to-radiotherapy
#19
Roshan A Karunamuni, Tyler M Seibert, Nathan S White, Linda McEvoy, Nikdokht Farid, James Brewer, Anders M Dale, Carrie R McDonald, Jona A Hattangadi-Gluth
BACKGROUND: Radiation-induced cognitive impairment may be mediated by hippocampal damage, but the structural integrity of this region in tumor patients at baseline is unclear. Hippocampal volumes of 31 glioma patients prior to receiving radiotherapy were compared to a group of 34 healthy controls. MATERIALS AND METHODS: Left and right hippocampi on T1-weighted pre-contrast magnetic resonance images were automatically segmented using Freesurfer, and visually inspected for segmentation errors...
February 3, 2017: Acta Oncologica
https://www.readbyqxmd.com/read/28151457/effectiveness-of-stapedotomy-in-improving-hearing-sensitivity-for-53-otosclerotic-patients-retrospective-review
#20
Farid Alzhrani, Mohammad M Mokhatrish, Murad O Al-Momani, Hassan Alshehri, Abdulrahman Hagr, Soha N Garadat
BACKGROUND: Otosclerosis is a common cause of progressive hearing impairment that causes fixation of the stapes. Surgical intervention is the preferred treatment approach to ameliorate the conductive hearing loss associated with stapedial otosclerosis. However, given that it is a difficult and delicate procedure, the surgery may fail for a number of reasons. Therefore, it is very important to evaluate the success rate of the surgical approach used in each regional center. OBJECTIVE: To examine the effectiveness of stapedotomy in improving hearing sensitivity for otosclerotic patients at King Abdul Aziz University Hospital in Riyadh...
January 2017: Annals of Saudi Medicine
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