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Antibiotic Efflux

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https://www.readbyqxmd.com/read/28332827/subcellular-chemical-imaging-of-antibiotics-in-single-bacteria-using-c60-secondary-ion-mass-spectrometry
#1
Hua Tian, David A Six, Thomas Krucker, Jennifer A Leeds, Nicholas Winograd
The inherent difficulty of discovering new and effective antibacterials and the rapid development of resistance particularly in Gram-negative bacteria, illustrates the urgent need for new methods that enable rational drug design. Here we report the development of 3D imaging cluster secondary ion mass spectrometry (SIMS) as a label-free approach to chemically map small molecules in aggregated and single Escherichia coli cells, with ~300 nm spatial resolution and high chemical sensitivity. The feasibility of quantitative analysis was explored, and a non-linear relationship between treatment dose and signal for tetracycline and ampicillin, two clinically-used antibacterials, was observed...
March 23, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28331838/bibliometric-analysis-of-literature-on-antifungal-triazole-resistance-1980-2015
#2
Waleed M Sweileh, Ansam F Sawalha, Samah Al-Jabi, Sa'ed H Zyoud
BACKGROUND: Triazole antifungal agents play an important role in the treatment of a wide range of fungal infections. Little is known about antifungal triazole drug resistance when compared to antibiotic resistance. Therefore, this study was carried out to give a bibliometric overview of literature on triazole antifungal drug resistance. METHODS: Keywords related to triazole drug class and resistance were used in a search query in the Scopus search engine. The time span was set from 1980 to 2015...
March 2017: Germs
https://www.readbyqxmd.com/read/28303897/synergistic-efficacy-of-bisbenzimidazole-and-carbonyl-cyanide-3-chlorophenylhydrazone-combination-against-mdr-bacterial-strains
#3
Devapriya Sinha, Stuti Pandey, Raja Singh, Vinod Tiwari, Kirti Sad, Vibha Tandon
Activation of efflux systems and the formation of biofilm are majorly adapted by microbes to resist antimicrobial agents. PPEF (bisbenzimidazole) targeting topoisomerase IA is observed to be an effective bactericidal agent against both Gram-positive and Gram-negative bacterial strains and thus can be developed as potent broad-spectrum antibiotic against MDR strains. PPEF treatment did not cause target specific mutation instead it leads to up-regulation of efflux gene in E. coli K12 as a mechanism of resistance...
March 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28302074/clonality-outer-membrane-proteins-profile-and-efflux-pump-in-kpc-producing-enterobacter-sp-in-brazil
#4
Juliana Ferraz Rosa, Camila Rizek, Ana Paula Marchi, Thais Guimaraes, Lourdes Miranda, Claudia Carrilho, Anna S Levin, Silvia F Costa
BACKGROUND: Carbapenems resistance in Enterobacter spp. has increased in the last decade, few studies, however, described the mechanisms of resistance in this bacterium. This study evaluated clonality and mechanisms of carbapenems resistance in clinical isolates of Enterobacter spp. identified in three hospitals in Brazil (Hospital A, B and C) over 7-year. METHODS: Antibiotics sensitivity, pulsed-field gel electrophoresis (PFGE), PCR for carbapenemase and efflux pump genes were performed for all carbapenems-resistant isolates...
March 17, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28294992/new-roads-leading-to-old-destinations-efflux-pumps-as-targets-to-reverse-multidrug-resistance-in-bacteria
#5
REVIEW
Gabriella Spengler, Annamária Kincses, Márió Gajdács, Leonard Amaral
Multidrug resistance (MDR) has appeared in response to selective pressures resulting from the incorrect use of antibiotics and other antimicrobials. This inappropriate application and mismanagement of antibiotics have led to serious problems in the therapy of infectious diseases. Bacteria can develop resistance by various mechanisms and one of the most important factors resulting in MDR is efflux pump-mediated resistance. Because of the importance of the efflux-related multidrug resistance the development of new therapeutic approaches aiming to inhibit bacterial efflux pumps is a promising way to combat bacteria having over-expressed MDR efflux systems...
March 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28291698/detection-and-analysis-of-different-interactions-between-resistance-mechanisms-and-carbapenems-in-clinical-isolates-of-klebsiella-pneumoniae
#6
Tanise Vendruscolo Dalmolin, Bianca Vendruscolo Bianchini, Grazielle Guidolin Rossi, Ana Carolina Ramos, Ana Cristina Gales, Priscila de Arruda Trindade, Marli Matiko Anraku de Campos
Carbapenems are considered last-line agents for the treatment of serious infections caused by Klebsiella pneumoniae, and this microorganism may exhibit resistance to β-lactam antibiotics due to different mechanisms of resistance. We evaluated 27 isolates of K. pneumoniae resistant to carbapenems recovered from inpatients at the University Hospital of Santa Maria-RS from July 2013 to August 2014. We carried out antimicrobial susceptibility, carbapenemase detection, testing for the presence of efflux pump by broth microdilution and loss of porin by sodium dodecyl sulfate polyacrylamide gel electrophoresis...
February 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28289081/light-modulates-metabolic-pathways-and-other-novel-physiological-traits-in-the-human-pathogen-acinetobacter-baumannii
#7
Gabriela L Müller, Marisel Tuttobene, Matías Altilio, Maitena Martinez Amezaga, Meaghan Nguyen, P Pamela Cribb, Larisa E Cybulski, María Soledad Ramírez, Silvia Altabe, María Alejandra Mussi
Light sensing in chemotrophic bacteria has been relatively recently ascertained. In the human pathogen Acinetobacter baumannii, light modulates motility, biofilm formation and virulence through the BLUF photoreceptor BlsA. In addition, light can induce reduction in susceptibility to certain antibiotics such as minocycline and tigecycline in a photoreceptor-independent manner. In this work we identified new traits whose expression are modulated by light in this pathogen, which comprise not only important determinants related to pathogenicity and antibiotic resistance, but also metabolic pathways, which represents a novel concept for chemotrophic bacteria...
March 13, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28284638/does-antifouling-paint-select-for-antibiotic-resistance
#8
Carl-Fredrik Flach, Chandan Pal, Carl Johan Svensson, Erik Kristiansson, Marcus Östman, Johan Bengtsson-Palme, Mats Tysklind, D G Joakim Larsson
There is concern that heavy metals and biocides contribute to the development of antibiotic resistance via co-selection. Most antifouling paints contain high amounts of such substances, which risks turning painted ship hulls into highly mobile refuges and breeding grounds for antibiotic-resistant bacteria. The objectives of this study were to start investigate if heavy-metal based antifouling paints can pose a risk for co-selection of antibiotic-resistant bacteria and, if so, identify the underlying genetic basis...
March 8, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28284092/antibiotic-resistant-pseudomonas-spp-in-the-aquatic-environment-a-prevalence-study-under-tropical-and-temperate-climate-conditions
#9
Naresh Devarajan, Thilo Köhler, Periyasamy Sivalingam, Christian van Delden, Crispin K Mulaji, Pius T Mpiana, Bastiaan W Ibelings, John Poté
Microbial populations which are resistant to antibiotics are an emerging environmental concern with potentially serious implications for public health. Thus, there is a growing concern in exploring the occurrence of antibiotic resistance in the environment with no limitations to the factors that contribute to their emergence. The aquatic environment is considered to be a hot-spot for the acquisition and spread of antibiotic resistance due to pollution with emerging contaminants derived from anthropogenic activities...
February 27, 2017: Water Research
https://www.readbyqxmd.com/read/28283780/metabolic-carbonyl-reduction-of-anthracyclines-role-in-cardiotoxicity-and-cancer-resistance-reducing-enzymes-as-putative-targets-for-novel-cardioprotective-and-chemosensitizing-agents
#10
REVIEW
Kamil Piska, Paulina Koczurkiewicz, Adam Bucki, Katarzyna Wójcik-Pszczoła, Marcin Kołaczkowski, Elżbieta Pękala
Anthracycline antibiotics (ANT), such as doxorubicin or daunorubicin, are a class of anticancer drugs that are widely used in oncology. Although highly effective in cancer therapy, their usefulness is greatly limited by their cardiotoxicity. Possible mechanisms of ANT cardiotoxicity include their conversion to secondary alcohol metabolites (i.e. doxorubicinol, daunorubicinol) catalyzed by carbonyl reductases (CBR) and aldo-keto reductases (AKR). These metabolites are suspected to be more cardiotoxic than their parent compounds...
March 10, 2017: Investigational New Drugs
https://www.readbyqxmd.com/read/28275586/colistin-resistance-in-acinetobacter-baumannii-mdr-zj06-revealed-by-a-multiomics-approach
#11
Xiaoting Hua, Lilin Liu, Youhong Fang, Qiucheng Shi, Xi Li, Qiong Chen, Keren Shi, Yan Jiang, Hua Zhou, Yunsong Yu
Acinetobacter baumannii has emerged as an important opportunistic pathogen due to its ability to acquire resistance to most currently available antibiotics. Colistin is often considered as the last line of therapy for infections caused by multidrug-resistant A. baumannii (MDRAB). However, colistin-resistant A. baumannii strain has recently been reported. To explore how multiple drug-resistant A. baumannii responded to colistin resistance, we compared the genomic, transcriptional and proteomic profile of A. baumannii MDR-ZJ06 to the induced colistin-resistant strain ZJ06-200P5-1...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28275296/effect-of-biofilm-formation-by-clinical-isolates-of-helicobacter-pylori-on-the-efflux-mediated-resistance-to-commonly-used-antibiotics
#12
Bahareh Attaran, Tahereh Falsafi, Nassim Ghorbanmehr
AIM: To evaluate the role of biofilm formation on the resistance of Helicobacter pylori (H. pylori) to commonly prescribed antibiotics, the expression rates of resistance genes in biofilm-forming and planktonic cells were compared. METHODS: A collection of 33 H. pylori isolates from children and adult patients with chronic infection were taken for the present study. The isolates were screened for biofilm formation ability, as well as for polymerase chain reaction (PCR) reaction with HP1165 and hp1165 efflux pump genes...
February 21, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28273168/within-host-whole-genome-analysis-of-an-antibiotic-resistant-pseudomonas-aeruginosa-strain-sub-type-in-cystic-fibrosis
#13
Laura J Sherrard, Anna S Tai, Bryan A Wee, Kay A Ramsay, Timothy J Kidd, Nouri L Ben Zakour, David M Whiley, Scott A Beatson, Scott C Bell
A Pseudomonas aeruginosa AUST-02 strain sub-type (M3L7) has been identified in Australia, infects the lungs of some people with cystic fibrosis and is associated with antibiotic resistance. Multiple clonal lineages may emerge during treatment with mutations in chromosomally encoded antibiotic resistance genes commonly observed. Here we describe the within-host diversity and antibiotic resistance of M3L7 during and after antibiotic treatment of an acute pulmonary exacerbation using whole genome sequencing and show both variation and shared mutations in important genes...
2017: PloS One
https://www.readbyqxmd.com/read/28272881/role-of-aromatic-and-negatively-charged-residues-of-drrb-in-multi-substrate-specificity-conferred-by-the-drrab-system-of-streptomyces-peucetius
#14
Kenneth Brown, Wen Li, Parjit Kaur
Resistance to the anticancer antibiotics, doxorubicin and daunorubicin, in the producer organism Streptomyces peucetius is conferred by an ABC transporter made of two proteins DrrA and DrrB, which together form a dedicated exporter for these two antibiotics. Surprisingly, however, the DrrAB system exhibits broad substrate specificity overlapping with well-studied multidrug resistance transporters, including P-glycoprotein. Therefore it provides an excellent model to study molecular basis of multi-specificity in a prototype efflux system with the potential to unravel the origin and evolution of multidrug resistance...
March 8, 2017: Biochemistry
https://www.readbyqxmd.com/read/28270804/eradication-and-sensitization-of-methicillin-resistant-staphylococcus-aureus-to-methicillin-with-bioactive-extracts-of-berry-pomace
#15
Serajus Salaheen, Mengfei Peng, Jungsoo Joo, Hironori Teramoto, Debabrata Biswas
The therapeutic roles of phenolic blueberry (Vaccinium corymbosum) and blackberry (Rubus fruticosus) pomace (commercial byproduct) extracts (BPE) and their mechanism of actions were evaluated against methicillin resistant Staphylococcus aureus (MRSA). Five major phenolic acids of BPE, e.g., protocatechuic, p. coumaric, vanillic, caffeic, and gallic acids, as well as crude BPE completely inhibited the growth of vegetative MRSA in vitro while BPE+methicillin significantly reduced MRSA biofilm formation on plastic surface...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28265005/metabolic-constraints-on-the-evolution-of-antibiotic-resistance
#16
Mattia Zampieri, Tim Enke, Victor Chubukov, Vito Ricci, Laura Piddock, Uwe Sauer
Despite our continuous improvement in understanding antibiotic resistance, the interplay between natural selection of resistance mutations and the environment remains unclear. To investigate the role of bacterial metabolism in constraining the evolution of antibiotic resistance, we evolved Escherichia coli growing on glycolytic or gluconeogenic carbon sources to the selective pressure of three different antibiotics. Profiling more than 500 intracellular and extracellular putative metabolites in 190 evolved populations revealed that carbon and energy metabolism strongly constrained the evolutionary trajectories, both in terms of speed and mode of resistance acquisition...
March 6, 2017: Molecular Systems Biology
https://www.readbyqxmd.com/read/28261566/efflux-pump-overexpression-contributes-to-tigecycline-heteroresistance-in-salmonella-enterica-serovar-typhimurium
#17
Yi Chen, Daxing Hu, Qijing Zhang, Xiao-Ping Liao, Ya-Hong Liu, Jian Sun
Bacterial heteroresistance has been identified in several combinations of bacteria and antibiotics, and it complicated the therapeutic strategies. Tigecycline is being used as one of the optimal options for the treatment of infections caused by multidrug-resistant Salmonella. This study investigated whether heterorresistance to tigecycline exists in a Salmonella enterica serovar Typhimurium strain harboring the oqxAB-bearing IncHI2 plasmid pHXY0908. MIC and population analyses were performed to evaluate population-wide susceptibility to tigecycline...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28258237/computational-modelling-of-efflux-pumps-and-their-inhibitors
#18
REVIEW
Venkata Krishnan Ramaswamy, Pierpaolo Cacciotto, Giuliano Malloci, Attilio V Vargiu, Paolo Ruggerone
Antimicrobial resistance is based on the multifarious strategies that bacteria adopt to face antibiotic therapies, making it a key public health concern of our era. Among these strategies, efflux pumps (EPs) contribute significantly to increase the levels and profiles of resistance by expelling a broad range of unrelated compounds - buying time for the organisms to develop specific resistance. In Gram-negative bacteria, many of these chromosomally encoded transporters form multicomponent 'pumps' that span both inner and outer membranes and are driven energetically by a primary or secondary transporter component...
February 28, 2017: Essays in Biochemistry
https://www.readbyqxmd.com/read/28258232/insights-into-drug-resistance-mechanisms-in-clostridium-difficile
#19
REVIEW
Phurt Harnvoravongchai, Methinee Pipatthana, Surang Chankhamhaengdecha, Tavan Janvilisri
The incidence of Clostridium difficile infection has been elevated and becoming common in hospitals worldwide. Although antibiotics usually serve as the primary treatment for bacterial infection including C. difficile infection, limitations and failures have been evident due to drug resistance. Antibiotic resistance in C. difficile has been recognized as one of the most important factors to promote the infection and increase the level of severity and the recurrence rate. Several outbreaks in many countries have been linked to the emergence of hypervirulent drug-resistant strains...
February 28, 2017: Essays in Biochemistry
https://www.readbyqxmd.com/read/28258149/salmonella-senftenberg-adaptation-to-linalool-and-its-association-with-antibiotic-resistance-and-environmental-persistence
#20
Emmanuel Kalily, Amit Hollander, Ben Korin, Itamar Cymerman, Sima Yaron
A clinical isolate of Salmonella enterica serovar Senftenberg, isolated from an outbreak linked to the herb Ocimum basilicum L. (basil), has been shown resistance to basil oil and to the terpene alcohol linalool. To better understand how human pathogens might develop resistance to linalool and to investigate the association of this resistance with resistance to different antimicrobial agents, selective pressure was applied to the wild-type strain by sequential exposure to increasing concentrations of linalool...
March 3, 2017: Applied and Environmental Microbiology
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