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https://www.readbyqxmd.com/read/29436118/cortistatin%C3%A2-inhibits%C3%A2-arterial%C3%A2-calcification%C3%A2-in%C3%A2-rats%C3%A2-via%C3%A2-gsk3%C3%AE-%C3%AE-catenin%C3%A2-and%C3%A2-pkc%C3%A2-signaling%C3%A2-but%C3%A2-not%C3%A2-jnk%C3%A2-signaling
#1
Yue Liu, Fang Lin, Yu Fu, Wenjia Chen, Wenxiu Liu, Jinyu Chi, Xiaohui Zhang, Xinhua Yin
AIM: Cortistatin (CST) is a newly discovered endogenous active peptide that exerts protective effects on the cardiovascular system. However, the relationship between CST and aortic calcification and the underlying mechanism remain obscure. Therefore, we investigated effects of CST on aortic calcification and its signaling pathways. METHODS: Calcium content and alkaline phosphatase (ALP) activity were measured using the o-cresolphthalein colorimetric method and ALP assay kit, respectively...
February 13, 2018: Acta Physiologica
https://www.readbyqxmd.com/read/29433109/pathogenesis-of-aortic-wall-complications-in-marfan-syndrome
#2
Nimrat Grewal, Adriana C Gittenberger-de Groot
BACKGROUND: Patients with Marfan (MFS) syndrome and patients with a bicuspid aortic valve (BAV) are more prone to develop aortic dilation and dissection compared to persons with a tricuspid aortic valve (TAV). To elucidate potential common as well as distinct pathways of clinical relevance, we compared the histopathological substrates of aortic pathology. PATIENT AND METHODS: Ascending aortic wall specimen were divided in five groups: BAV (n=36) and TAV (n=23) without and with dilation and non-dilated MFS (n=8)...
February 2, 2018: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/29415538/laminaria-japonica-polysaccharide-inhibits-vascular-calcification-via-preventing-osteoblastic-differentiation-of-vascular-smooth-muscle-cells
#3
Xue-Ying Li, Qiang-Ming Li, Qing Fang, Xue-Qiang Zha, Panlihua Panlihua, Jian-Ping Luo
This study aimed to investigate the effect and underlying mechanism of a purified Laminaria japonica polysaccharide (LJP61A) on preventing vascular calcification (VC). In adenine-induced chronic renal failure (CRF) mice VC model and β-glycerophosphate (β-GP)-induced vascular smooth muscle cells (VSMC) calcification model, LJP61A was found to significantly inhibit VC phenotypes as determined by biochemical analysis, von Kossa, alizarin red and immunohistochemical staining. Meanwhile, LJP61A remarkably up-regulated the mRNA levels of VSMC related markers and down-regulated the mRNA levels of sodium-dependent phosphate cotransporter Pit-1...
February 8, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29402827/expanded-haemodialysis-therapy-of-chronic-haemodialysis-patients-prevents-calcification-and-apoptosis-of-vascular-smooth-muscle-cells-in-vitro
#4
Kevin Willy, Matthias Girndt, Jakob Voelkl, Roman Fiedler, Peter Martus, Markus Storr, Ralf Schindler, Daniel Zickler
BACKGROUND: Vascular calcification is a common phenomenon in patients with chronic kidney disease and strongly associated with increased cardiovascular mortality. Vascular calcification is an active process mediated in part by inflammatory processes in vascular smooth muscle cells (VSMC). These could be modified by the insufficient removal of proinflammatory cytokines through conventional high-flux (HF) membranes. Recent trials demonstrated a reduction of inflammation in VSMC by use of dialysis membranes with a higher and steeper cut-off...
December 22, 2017: Blood Purification
https://www.readbyqxmd.com/read/29362439/deletion-of-dgcr8-in-vsmcs-of-adult-mice-results-in-loss-of-vascular-reactivity-reduced-blood-pressure-and-neointima-formation
#5
Yanan Zou, Zixuan Chen, Brett L Jennings, Guannan Zhao, Qingqing Gu, Anindya Bhattacharya, Yan Cui, Bo Yu, Kafait U Malik, Junming Yue
DiGeorge syndrome chromosomal region 8 (DGCR8), a double-stranded-RNA-binding protein, participates in the miRNA biogenesis pathway and contributes to miRNA maturation by interacting with the RNAase III enzyme Drosha in cell nuclei. To investigate the role of DGCR8 in vascular smooth muscle cells (VSMCs) at the postnatal stages, we generated tamoxifen-inducible VSMC specific knockout (iKO) mice by crossing DGCR8loxp/loxp with VSMC specific tamoxifen-inducible Cre transgenic mice SMA-Cre-ERT2. DGCR8iKO mice display reduced body weight one month following tamoxifen treatment and died around 3 months...
January 23, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29361366/physiologic-levels-of-resistin-induce-a-shift-from-proliferation-to-apoptosis-in-macrophage-and-vsmc-co-culture
#6
Mary C Zuniga, Gayatri Raghuraman, Wei Zhou
BACKGROUND: Resistin, an adipokine with inflammatory properties, has been associated with plaque vulnerability. Vascular smooth muscle cells and macrophages are the major cellular components in advanced atherosclerotic plaques and interdependently affect plaque stability. The purpose of this study was to examine the effects of resistin on the interactions of vascular smooth muscle cells and macrophages using co-culture systems. METHODS: Human monocytes were differentiated into macrophages...
January 17, 2018: Surgery
https://www.readbyqxmd.com/read/29310814/microarray-expression-profile-analysis-of-long-non-coding-rnas-in-thoracic-aortic-aneurysm
#7
Yang Li, Nan Yang
Thoracic aortic aneurysm (TAA) is a highly lethal vascular disease. Long non-coding RNAs (lncRNAs) are newly discovered as a regulator of protein genes and play critical roles in cardiovascular physio-pathological processes. However, there were a few studies looking at lncRNAs in TAA. In this study, we profiled differential expression of lncRNAs between TAA (TAA group, N = 6) and normal thoracic aorta (control group, n = 6) by third-generation lncRNA microarray. We identified 1352 up-regulated and 1624 down-regulated lncRNAs with differential expression (log fold-change > 2...
January 2018: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/29246895/mirna-22-is-a-novel-mediator-of-vascular-smooth-muscle-cell-phenotypic-modulation-and-neointima-formation
#8
Feng Yang, Qishan Chen, Shiping He, Mei Yang, Eithne Margaret Maguire, Weiwei An, Tayyab Adeel Afzal, Le Anh Luong, Li Zhang, Qingzhong Xiao
Background -MicroRNA-22 (miR-22) has recently been reported to play a regulatory role during vascular smooth muscle cell (VSMC) differentiation from stem cells, but little is known about its target genes and related pathways in mature VSMC phenotypic modulation or its clinical implication in neointima formation following vascular injury. Methods -We applied wire-injury mouse model as well as local delivery of AgomiR-22 or miR-22 inhibitor to explore the therapeutic potential of miR-22 in vascular diseases. Furthermore, normal and diseased human femoral arteries were harvested and various in vivo, ex vivo, and in vitro models of VSMC phenotype switching were conducted to examine miR-22 expression during VSMC phenotype switching...
December 15, 2017: Circulation
https://www.readbyqxmd.com/read/29238011/plaque-calcification-during-atherosclerosis-progression-and-regression
#9
Atsushi Shioi, Yuji Ikari
Plaque calcification develops by the inflammation-dependent mechanisms involved in progression and regression of atherosclerosis. Macrophages can undergo two distinct polarization states, that is, pro-inflammatory M1 phenotype in progression and anti-inflammatory M2 phenotype in regression. In plaque progression, predominant M1 macrophages promote the initial calcium deposition within the necrotic core of the lesions, called as microcalcification, through not only vesicle-mediated mineralization as the result of apoptosis of macrophages and vascular smooth muscle cells (VSMCs), but also VSMC differentiation into early phase osteoblasts...
December 12, 2017: Journal of Atherosclerosis and Thrombosis
https://www.readbyqxmd.com/read/29232926/differential-effects-of-seh-inhibitors-on-the-proliferation-and-migration-of-vascular-smooth-muscle-cells
#10
Hyo Seon Kim, Sang Kyum Kim, Keon Wook Kang
Epoxyeicosatrienoic acid (EET) is a cardioprotective metabolite of arachidonic acid. It is known that soluble epoxide hydrolase (sEH) is involved in the metabolic degradation of EET. The abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) play important roles in the pathogenesis of atherosclerosis and restenosis. Thus, the present study investigated the effects of the sEH inhibitor 12-(((tricyclo(3.3.1.13,7)dec-1-ylamino)carbonyl)amino)-dodecanoic acid (AUDA) on platelet-derived growth factor (PDGF)-induced proliferation and migration in rat VSMCs...
December 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29228352/tumour-necrosis-factor-alpha-in-uraemic-serum-promotes-osteoblastic-transition-and-calcification-of-vascular-smooth-muscle-cells-via-extracellular-signal-regulated-kinases-and-activator-protein-1-c-fos-mediated-induction-of-interleukin-6-expression
#11
Daniel Zickler, Christian Luecht, Kevin Willy, Lei Chen, Janusz Witowski, Matthias Girndt, Roman Fiedler, Markus Storr, Julian Kamhieh-Milz, Janosch Schoon, Sven Geissler, Olle Ringdén, Ralf Schindler, Guido Moll, Duska Dragun, Rusan Catar
Background: Vascular calcification is enhanced in uraemic chronic haemodialysis patients, likely due to the accumulation of midsize uraemic toxins, such as interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α). Here we have assessed the impact of uraemia on vascular smooth muscle cell (VSMC) calcification and examined the role of IL-6 and TNF-α as possible mediators and, most importantly, its underlying signalling pathway in VSMCs. Methods: VSMCs were incubated with samples of uraemic serum obtained from patients treated with haemodialysis for renal failure in the Permeability Enhancement to Reduce Chronic Inflammation-I clinical trial...
December 8, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/29227975/high-phosphate-induced-calcification-of-vascular-smooth-muscle-cells-is-associated-with-the-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway
#12
Daohai Zhang, Xianjin Bi, Yong Liu, Yinghui Huang, Jiachuan Xiong, Xinli Xu, Tangli Xiao, Yanlin Yu, Wei Jiang, Yunjian Huang, Jingbo Zhang, Bo Zhang, Jinghong Zhao
BACKGROUND/AIMS: Hyperphosphatemia is one of the most notable features of chronic kidney disease (CKD). Numerous epidemiological and clinical studies have found that high serum phosphate concentrations are associated with calcification in the coronary arteries. However, the mechanisms underlying the vascular calcification induced by high phosphate have not been understood fully. METHODS: Vascular smooth muscle cells (VSMCs) were cultured in high-phosphate media to induce vascular calcification, which was detected by Alizarin red S staining...
December 8, 2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29212792/differential-effects-of-alcohol-and-its-metabolite-acetaldehyde-on-vascular-smooth-muscle-cell-notch-signaling-and-growth
#13
Ekaterina Hatch, David Morrow, Weimin Liu, Paul A Cahill, Eileen M Redmond
Alcohol (EtOH) consumption can variously affect cardiovascular disease (CVD). Our aim was to compare the effects of EtOH and its primary metabolite acetaldehyde (ACT) on vascular smooth muscle Notch signaling and cell growth that are important for atherogenesis. Human coronary artery smooth muscle cells (HCASMC) were treated with EtOH (25 mM) or ACT (10 μM, 25 μM). As previously reported, EtOH inhibited Notch signaling and growth of HCASMC. In contrast, ACT treatment stimulated HCASMC proliferation (cell counts) and increased proliferating cell nuclear antigen (PCNA) expression, concomitant with stimulation of Notch signaling as determined by increased Notch receptor (N1, N3) and target gene (HRT 1-3) mRNA levels...
December 6, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29152140/inhibition-of-il-8-mediated-endothelial-adhesion-vsmcs-proliferation-and-migration-by-sirna-tmem98-suggests-tmem98-s-emerging-role-in-atherosclerosis
#14
Guangxin Lv, Hongmei Zhu, Cai Li, Jingyu Wang, Dandan Zhao, Shuyao Li, Le Ma, Guohua Sun, Fang Li, Ying Zhao, Ying Gao
Transmembrane protein 98 (TMEM98), known as a novel gene related to lung cancer, hepatocellular carcinoma, differentiation of T helper 1 cells and normal eye development, has no defined role reported in terms of atherosclerosis (AS). To investigate the potential involvement of TMEM98 during AS processes, its obvious secretion and expression has been initially characterized in hyperlipidemia patients' serum and AS mice's serum respectively. We then explored the possible role of TMEM98 in the pathogenesis of AS in vitro...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29104843/silencing-mir-16-expression-promotes-angiotensin-ii-stimulated-vascular-smooth-muscle-cell-growth
#15
Qingqing Gu, Guannan Zhao, Yinan Wang, Biao Xu, Junming Yue
miRNAs are a class of non-coding endogenous small RNAs that control gene expression at the posttranscriptional level and involved in cell proliferation, migration and differentiation. Dysregulation of miRNA expression is involved in a variety of human diseases including cardiovascular diseases. miRNAs have been shown to regulate vascular smooth muscle cell (VSMC) function and play vital roles in hypertension, restenosis and atherosclerosis. Here we reported that miR-16 as one of miRNAs in the miR-15 family was highly expressed in vascular smooth muscle cells (VSMCs) and involved in angiotensin II (Ang II) mediated VSMC signaling pathways...
March 2017: Cell & Developmental Biology
https://www.readbyqxmd.com/read/29100354/mechanism-of-genistein-regulating-the-differentiation-of-vascular-smooth-muscle-cells-into-osteoblasts-via-the-opg-rankl-pathway
#16
Cheng Shen, Ye Yuan, Fuping Li, Yijie Hu, Yi Song, Shulin Zhao, Qianjin Zhong
Objective: The present study aimed to investigate the mechanism of genistein, a tyrosine kinase inhibitor, regulating the differentiation of vascular smooth muscle cells (VSMCs) into osteoblasts via the OPG/RANKL (Osteoprotegerin/Receptor Activator of Nuclear Factor-κB Ligand) pathway. Methods: The mouse VSMCs were isolated, purified and cultured. We constructed the LV5-Tnfrsf11b overexpression lentiviral vector and LV3-OPG-309 interference lentiviral vector. The OPG overexpression was induced and the growth of VSMCs infected with the lentiviral vector was observed...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29100273/mir-26a-contributes-to-the-pdgf-bb-induced-phenotypic-switch-of-vascular-smooth-muscle-cells-by-suppressing-smad1
#17
Xiaoyan Yang, Mei Dong, Hao Wen, Xiaoling Liu, Meng Zhang, Lianyue Ma, Cheng Zhang, Xiaorong Luan, Huixia Lu, Yun Zhang
The phenotypic switch of vascular smooth muscle cells (VSMCs) is a key event in the pathogenesis of various vascular diseases, such as atherosclerosis and post-angioplasty restenosis. Small non-coding microRNAs (miRNAs) have emerged as critical modulators of VSMC function. In the present study, miR-26a was significantly increased in cultured VSMCs stimulated by platelet-derived growth factor-BB (PDGF-BB) and in arteries with neointimal lesion formation. Moreover, we demonstrated that miR-26a regulates the expression of VSMC differentiation marker genes such as α-smooth muscle actin (α-SMA), calponin and smooth muscle myosin heavy chain (SM-MHC) in PDGF-BB-treated VSMCs...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29089350/unspliced-xbp1-confers-vsmc-homeostasis-and-prevents-aortic-aneurysm-formation-via-foxo4-interaction
#18
Guizhen Zhao, Yi Fu, Zeyu Cai, Fang Yu, Ze Gong, Rongbo Dai, Yanhua Hu, Lingfang Zeng, Qingbo Xu, Wei Kong
RATIONALE: Although not fully understood, the phenotypic transition of vascular smooth muscle cells exhibits at the early onset of the pathology of aortic aneurysms. Exploring the key regulators that are responsible for maintaining the contractile phenotype of vascular smooth muscle cells (VSMCs) may confer vascular homeostasis and prevent aneurysmal disease. XBP1 (X-box binding protein 1), which exists in a transcriptionally inactive unspliced form (XBP1u) and a spliced active form (XBP1s), is a key component in response to endoplasmic reticular stress...
December 8, 2017: Circulation Research
https://www.readbyqxmd.com/read/29079194/role-of-ros-trpm7-erk1-2-axis-in-high-concentration-glucose-mediated-proliferation-and-phenotype-switching-of-rat-aortic-vascular-smooth-muscle-cells
#19
Meimei Yang, Jing Fang, Qingan Liu, Yan Wang, Zhuobo Zhang
This study investigated the change of transient receptor potential cation channel subfamily M member 7 (TRPM7) expression in rat aortic vascular smooth muscle cells (RAoSMCs) treated with a high concentration of d-glucose (HG) and its role in promoting the proliferative phenotype of RAoSMCs. Chronic exposure to HG increased TRPM7 protein expression and TRPM7 whole-cell currents in RAoSMCs. By contrast, RAoSMC exposure to high concentration of l-glucose and mannital exhibited no such effect. Mechanistically, HG treatment elevated TRPM7 expression by increasing oxidative stress...
October 25, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29021296/lactate-promotes-synthetic-phenotype-in-vascular-smooth-muscle-cells
#20
Libang Yang, Ling Gao, Thomas Nickel, Jing Yang, Jingyi Zhou, Adam Gilbertsen, Zhaohui Geng, Caitlin Johnson, Bernice Young, Craig Henke, Glenn R Gourley, Jianyi Zhang
RATIONALE: The phenotypes of vascular smooth muscle cells (vSMCs) comprise a continuum bounded by predominantly contractile and synthetic cells. Some evidence suggests that contractile vSMCs can assume a more synthetic phenotype in response to ischemic injury, but the mechanisms that activate this phenotypic switch are poorly understood. OBJECTIVE: To determine whether lactate, which increases in response to regional ischemia, may promote the synthetic phenotype in vSMCs...
November 10, 2017: Circulation Research
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