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https://www.readbyqxmd.com/read/29895327/conditioned-medium-from-bone-marrow-derived-mesenchymal-stem-cells-inhibits-vascular-calcification-through-blockade-of-the-bmp2-smad1-5-8-signaling-pathway
#1
Shuangshuang Wang, Siwang Hu, Jian Wang, Yahui Liu, Ruochi Zhao, Maoqing Tong, Hanbin Cui, Nan Wu, Xiaomin Chen
BACKGROUND: Arterial calcification is associated with cardiovascular disease as a complication of advanced atherosclerosis and is a significant contributor to cardiovascular morbidity and mortality. Osteoblastic differentiation of vascular smooth muscle cells (VSMCs) plays an important role in arterial calcification and is characterized by cellular necrosis, inflammation, and lipoprotein and phospholipid complexes, especially in atherosclerotic calcification. The conditioned medium from bone marrow-derived mesenchymal stem cells (MSC-CM) is well known as a rich source of autologous cytokines and is universally used for tissue regeneration in current clinical medicine...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29785051/hyaluronan-negatively-regulates-vascular-calcification-involving-bmp2-signaling
#2
Yonglun Kong, Qingchun Liang, Yanting Chen, Pingzhen Yang, Xiaoyu Liu, Yining Li, Siyuan Feng, Ji Wu, Wantao Liu, Jingyi Tang, Huimin Yu, Jing-Song Ou, Lihe Lu, Jianyun Yan
Vascular calcification is a highly regulated biological process similar to bone formation involving osteogenic differentiation of vascular smooth muscle cells (VSMCs). Hyaluronan (HA), a major structural component of the extracellular matrix in cartilage, has been shown to inhibit osteoblast differentiation. However, whether HA affects osteogenic differentiation and calcification of VSMCs remains unclear. In the present study, we used in vitro and ex vivo models of vascular calcification to investigate the role of HA in vascular calcification...
May 21, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29738818/microrna-132-targeting-pten-contributes-to-cilostazol-promoted-vascular-smooth-muscle-cell-differentiation
#3
Wei-Jan Chen, Ying-Hwa Chen, Yu-Juei Hsu, Kwang-Huei Lin, Yung-Hsin Yeh
BACKGROUND AND AIMS: Cilostazol, beyond its antiplatelet effect, is also capable of promoting vascular smooth muscle cell (VSMC) differentiation. The aim of this study was to explore the potential role of PTEN, known to associate with VSMC differentiation, and its related microRNA (miRNA) in cilostazol-dependent effects. METHODS AND RESULTS: Microarray analysis in balloon-injured rat carotid arteries comparing with and without balloon injury revealed that miR-132 was differentially expressed...
April 26, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29721770/selenoprotein-s-inhibits-inflammation-induced-vascular-smooth-muscle-cell-calcification
#4
Yali Ye, Weixia Bian, Fen Fu, Jian Hu, Hongmei Liu
Vascular calcification is a prominent feature of many diseases including atherosclerotic cardiovascular disease (CVD), leading to high morbidity and mortality rates. A significant association of selenoprotein S (SelS) gene polymorphism with atherosclerotic CVD has been reported in epidemiologic studies, but the underlying mechanism is far from clear. To investigate the role of SelS in inflammation-induced vascular calcification, osteoblastic differentiation and calcification of vascular smooth muscle cells (VSMCs) induced by lipopolysaccharide (LPS) or tumor necrosis factor (TNF)-α were compared between the cells with and without SelS knockdown...
May 2, 2018: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/29717623/aortic-sca-1-sup-sup-progenitor-cells-arise-from-the-somitic-mesoderm-lineage-in-mouse
#5
Sarah K Steinbach, Tao Wang, Martha H Carruthers, Angela Li, Rickvinder Besla, Adam P Johnston, Clinton S Robbins, Mansoor Husain
Sca-1<sup>+</sup> progenitor cells in the adult mouse aorta are known to generate vascular smooth muscle cells (VSMC), but their embryological origins and temporal abundance are not known. Using tamoxifen-inducible <i>Myf5-Cre</i><sup>ER</sup> mice, we demonstrate that Sca-1<sup>+</sup> adult aortic cells arise from the somitic mesoderm beginning at E8.5 and continue throughout somitogenesis. <i>Myf5</i> lineage-derived Sca-1<sup>+</sup> cells greatly expand in situ starting at 4 weeks of age and become a major source of aortic Sca-1<sup>+</sup> cells by 6 weeks of age...
May 2, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29698390/microrna-expression-profile-and-functional-analysis-reveal-their-roles-in-contact-inhibition-and-its-disruption-switch-of-rat-vascular-smooth-muscle-cells
#6
Ye-Ying Sun, Shan-Shan Qin, Yun-Hui Cheng, Chao-Yun Wang, Xiao-Jun Liu, Ying Liu, Xiu-Li Zhang, Wendy Zhang, Jia-Xin Zhan, Shuai Shao, Wei-Hua Bian, Bi-Hui Luo, Dong-Feng Lu, Jian Yang, Chun-Hua Wang, Chun-Xiang Zhang
Contact inhibition and its disruption of vascular smooth muscle cells (VSMCs) are important cellular events in vascular diseases. But the underlying molecular mechanisms are unclear. In this study we investigated the roles of microRNAs (miRNAs) in the contact inhibition and its disruption of VSMCs and the molecular mechanisms involved. Rat VSMCs were seeded at 30% or 90% confluence. MiRNA expression profiles in contact-inhibited confluent VSMCs (90% confluence) and non-contact-inhibited low-density VSMCs (30% confluence) were determined...
May 2018: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/29689070/fti-277-inhibits-smooth-muscle-cell-calcification-by-up-regulating-pi3k-akt-signaling-and-inhibiting-apoptosis
#7
Arvind Ponnusamy, Smeeta Sinha, Gareth D Hyde, Samantha J Borland, Rebecca F Taylor, Emma Pond, Heather J Eyre, Colette A Inkson, Andrew Gilmore, Nick Ashton, Philip A Kalra, Ann E Canfield
BACKGROUND: Vascular calcification is associated with increased cardiovascular morbidity and mortality in patients with atherosclerosis, diabetes and chronic kidney disease. However, no viable treatments for this condition have been identified. This study aimed to determine whether farnesyl transferase inhibitors (FTIs) can reduce vascular calcification and the mechanism by which this reduction occurs. RESULTS: We demonstrate that FTI-277 significantly inhibits phosphate-induced mineral deposition by vascular smooth muscle cells (VSMC) in vitro, prevents VSMC osteogenic differentiation, and increases mRNA expression of matrix Gla protein (MGP), an inhibitor of mineralization...
2018: PloS One
https://www.readbyqxmd.com/read/29652803/hypoxia-supports-epicardial-cell-differentiation-in-vascular-smooth-muscle-cells-through-the-activation-of-the-tgf%C3%AE-pathway
#8
Jiayi Tao, Joey V Barnett, Michiko Watanabe, Diana Ramírez-Bergeron
Epicardium-derived cells (EPDCs) are an important pool of multipotent cardiovascular progenitor cells. Through epithelial-to-mesenchymal-transition (EMT), EPDCs invade the subepicardium and myocardium and further differentiate into several cell types required for coronary vessel formation. We previously showed that epicardial hypoxia inducible factor (HIF) signaling mediates the invasion of vascular precursor cells critical for patterning the coronary vasculature. Here, we examine the regulatory role of hypoxia (1% oxygen) on EPDC differentiation into vascular smooth muscle cells (VSMCs)...
April 13, 2018: Journal of Cardiovascular Development and Disease
https://www.readbyqxmd.com/read/29627363/nesfatin-1-functions-as-a-switch-for-phenotype-transformation-and-proliferation-of-vsmcs-in-hypertensive-vascular-remodeling
#9
Qing-Bo Lu, Hui-Ping Wang, Zi-Han Tang, Han Cheng, Qiong Du, Yuan-Ben Wang, Wu-Bing Feng, Ke-Xue Li, Wei-Wei Cai, Li-Ying Qiu, Hai-Jian Sun
The phenotypic transformation from differentiated to dedifferentiated vascular smooth muscle cells (VSMCs) plays a crucial role in VSMC proliferation and vascular remodeling in many cardiovascular diseases including hypertension. Nesfatin-1, a multifunctional adipocytokine, is critically involved in the regulation of blood pressure. However, it is still largely unexplored whether nesfatin-1 is a potential candidate in VSMC phenotypic switch and proliferation in hypertension. Experiments were carried out in Wistar-Kyoto rats (WKY), spontaneously hypertensive rats (SHR), human VSMCs and primary rat aortic VSMCs...
June 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29563538/ucma-grp-inhibits-phosphate-induced-vascular-smooth-muscle-cell-calcification-via-smad-dependent-bmp-signalling
#10
Brecht A Willems, Malgorzata Furmanik, Marjolein M J Caron, Martijn L L Chatrou, Dennis H M Kusters, Tim J M Welting, Michael Stock, Marta S Rafael, Carla S B Viegas, Dina C Simes, Cees Vermeer, Chris P M Reutelingsperger, Leon J Schurgers
Vascular calcification (VC) is the process of deposition of calcium phosphate crystals in the blood vessel wall, with a central role for vascular smooth muscle cells (VSMCs). VC is highly prevalent in chronic kidney disease (CKD) patients and thought, in part, to be induced by phosphate imbalance. The molecular mechanisms that regulate VC are not fully known. Here we propose a novel role for the mineralisation regulator Ucma/GRP (Upper zone of growth plate and Cartilage Matrix Associated protein/Gla Rich Protein) in phosphate-induced VSMC calcification...
March 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29558369/uhrf1-epigenetically-orchestrates-smooth-muscle-cell-plasticity-in-arterial-disease
#11
Leonardo Elia, Paolo Kunderfranco, Pierluigi Carullo, Marco Vacchiano, Floriana Maria Farina, Ignacio Fernando Hall, Stefano Mantero, Cristina Panico, Roberto Papait, Gianluigi Condorelli, Manuela Quintavalle
Adult vascular smooth muscle cells (VSMCs) dedifferentiate in response to extracellular cues such as vascular damage and inflammation. Dedifferentiated VSMCs are proliferative, migratory, less contractile, and can contribute to vascular repair as well as to cardiovascular pathologies such as intimal hyperplasia/restenosis in coronary artery and arterial aneurysm. We here demonstrate the role of ubiquitin-like containing PHD and RING finger domains 1 (UHRF1) as an epigenetic master regulator of VSMC plasticity...
May 7, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29547702/inhibition-of-enzymes-involved-in-collagen-cross-linking-reduces-vascular-smooth-muscle-cell-calcification
#12
Eva Jover, Ana Silvente, Francisco Marín, José Martínez-González, Mar Orriols, Carlos M Martinez, Carmen María Puche, Mariano Valdés, Cristina Rodriguez, Diana Hernández-Romero
Vascular smooth muscle cells (VSMCs) transdifferentiate into osteoblast-like cells during vascular calcification, inducing active remodeling and calcification of the extracellular matrix (ECM). Intracellular and extracellular enzymes, such as lysyl hydroxylase 1 (PLOD1) and lysyl oxidase (LOX), contribute to ECM maturation and stabilization. We assessed the contribution of these enzymes to hyperphosphatemia (HPM)-induced calcification. Human and murine VSMCs were differentiated into functional osteoblast-like cells by HPM conditioning...
March 16, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29538087/hsa-mir-320d-and-hsa-mir-582-mirna-biomarkers-of-aortic-dissection-regulate-apoptosis-of-vascular-smooth-muscle-cells
#13
Hong Shen, Shuyang Lu, Lili Dong, Yuan Xue, Chenling Yao, Chaoyang Tong, Chunsheng Wang, Xianhong Shu
Abnormal expression of microRNAs (miRNAs) has been associated with aortic dissection (AD). Next-generation sequencing was performed to identify the differentially expressed miRNAs in aortic tissue samples between AD and nondiseased individuals. Selected miRNAs, which showed significant variation between the 2 groups, were then transfected into human aortic vascular smooth muscle cells, and assessed for effects on cell migration and induced apoptosis. The changes in gene expression pattern in human aortic vascular smooth muscle cells transfected with the miRNAs were also investigated...
May 2018: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/29514202/role-of-smooth-muscle-cells-in-vascular-calcification-implications-in-atherosclerosis-and-arterial-stiffness
#14
Andrew L Durham, Mei Y Speer, Marta Scatena, Cecilia M Giachelli, Catherine M Shanahan
Vascular calcification is associated with a significant increase in all-cause mortality and atherosclerotic plaque rupture. Calcification has been determined to be an active process driven in part by vascular smooth muscle cell (VSMC) transdifferentiation within the vascular wall. Historically, VSMC phenotype switching has been viewed as binary, with the cells able to adopt a physiological contractile phenotype or an alternate 'synthetic' phenotype in response to injury. More recent work, including lineage tracing has however revealed that VSMCs are able to adopt a number of phenotypes, including calcific (osteogenic, chondrocytic, and osteoclastic), adipogenic, and macrophagic phenotypes...
March 15, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29484366/bag3-promotes-the-phenotypic-transformation-of-primary-rat-vascular-smooth-muscle-cells-via-trail
#15
Yao Fu, Ye Chang, Shuang Chen, Yuan Li, Yintao Chen, Guozhe Sun, Shasha Yu, Ning Ye, Chao Li, Yingxian Sun
Under normal physiological condition, the mature vascular smooth muscle cells (VSMCs) show differentiated phenotype. In response to various environmental stimuluses, VSMCs convert from the differentiated phenotype to dedifferentiated phenotype characterized by the increased ability of proliferation/migration and the reduction of contractile ability. The phenotypic transformation of VSMCs played an important role in atherosclerosis. Both Bcl-2-associated athanogene 3 (BAG3) and tumor necrosis factor-related apopt-osis inducing ligand (TRAIL) involved in apoptosis...
May 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29483881/phenotypic-modulation-of-cultured-primary-human-aortic-vascular-smooth-muscle-cells-by-uremic-serum
#16
Violeta Cazaña-Pérez, Pilar Cidad, Javier Donate-Correa, Ernesto Martín-Núñez, José R López-López, M Teresa Pérez-García, Teresa Giraldez, Juan F Navarro-González, Diego Alvarez de la Rosa
Patients with chronic kidney disease (CKD) have a markedly increased incidence of cardiovascular disease (CVD). The high concentration of circulating uremic toxins and alterations in mineral metabolism and hormone levels produce vascular wall remodeling and significant vascular damage. Medial calcification is an early vascular event in CKD patients and is associated to apoptosis or necrosis and trans-differentiation of vascular smooth muscle cells (VSMC) to an osteogenic phenotype. VSMC obtained from bovine or rat aorta and cultured in the presence of increased inorganic phosphate (Pi) have been extensively used to study these processes...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29436118/cortistatin-inhibits-arterial-calcification-in-rats-via-gsk3%C3%AE-%C3%AE-catenin-and-protein-kinase-c-signalling-but-not-c-jun-n-terminal-kinase-signalling
#17
Y Liu, F Lin, Y Fu, W Chen, W Liu, J Chi, X Zhang, X Yin
AIM: Cortistatin (CST) is a newly discovered endogenous active peptide that exerts protective effects on the cardiovascular system. However, the relationship between CST and aortic calcification and the underlying mechanism remain obscure. Therefore, we investigated effects of CST on aortic calcification and its signalling pathways. METHODS: Calcium content and alkaline phosphatase (ALP) activity were measured using the o-cresolphthalein colorimetric method and ALP assay kit respectively...
February 13, 2018: Acta Physiologica
https://www.readbyqxmd.com/read/29433109/pathogenesis-of-aortic-wall-complications-in-marfan-syndrome
#18
Nimrat Grewal, Adriana C Gittenberger-de Groot
BACKGROUND: Patients with Marfan (MFS) syndrome and patients with a bicuspid aortic valve (BAV) are more prone to develop aortic dilation and dissection compared to persons with a tricuspid aortic valve (TAV). To elucidate potential common as well as distinct pathways of clinical relevance, we compared the histopathological substrates of aortic pathology. PATIENT AND METHODS: Ascending aortic wall specimen were divided in five groups: BAV (n=36) and TAV (n=23) without and with dilation and non-dilated MFS (n=8)...
March 2018: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/29415538/laminaria-japonica-polysaccharide-inhibits-vascular-calcification-via-preventing-osteoblastic-differentiation-of-vascular-smooth-muscle-cells
#19
Xue-Ying Li, Qiang-Ming Li, Qing Fang, Xue-Qiang Zha, Li-Hua Pan, Jian-Ping Luo
This study aimed to investigate the effect and underlying mechanism of a purified Laminaria japonica polysaccharide (LJP61A) on preventing vascular calcification (VC). In the adenine-induced chronic renal failure (CRF) mice VC model and the β-glycerophosphate (β-GP)-induced vascular smooth muscle cells (VSMC) calcification model, LJP61A was found to significantly inhibit VC phenotypes as determined by biochemical analysis and von Kossa, alizarin red, and immunohistochemical staining. Meanwhile, LJP61A remarkably up-regulated the mRNA levels of VSMC related markers and down-regulated the mRNA levels of sodium-dependent phosphate cotransporter Pit-1...
February 28, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29402827/expanded-haemodialysis-therapy-of-chronic-haemodialysis-patients-prevents-calcification-and-apoptosis-of-vascular-smooth-muscle-cells-in-vitro
#20
Kevin Willy, Matthias Girndt, Jakob Voelkl, Roman Fiedler, Peter Martus, Markus Storr, Ralf Schindler, Daniel Zickler
BACKGROUND: Vascular calcification is a common phenomenon in patients with chronic kidney disease and strongly associated with increased cardiovascular mortality. Vascular calcification is an active process mediated in part by inflammatory processes in vascular smooth muscle cells (VSMC). These could be modified by the insufficient removal of proinflammatory cytokines through conventional high-flux (HF) membranes. Recent trials demonstrated a reduction of inflammation in VSMC by use of dialysis membranes with a higher and steeper cut-off...
2018: Blood Purification
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