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https://www.readbyqxmd.com/read/29212792/differential-effects-of-alcohol-and-its-metabolite-acetaldehyde-on-vascular-smooth-muscle-cell-notch-signaling-and-growth
#1
Ekaterina Hatch, David Morrow, Weimin Liu, Paul A Cahill, Eileen M Redmond
Alcohol (EtOH) consumption can variously affect cardiovascular disease (CVD). Our aim was to compare the effects of EtOH and its primary metabolite acetaldehyde (ACT) on vascular smooth muscle Notch signaling and cell growth that are important for atherogenesis. Human coronary artery smooth muscle cells (HCASMC) were treated with EtOH (25 mM) or ACT (10 μM, 25 μM). As previously reported, EtOH inhibited Notch signaling and growth of HCASMC. In contrast, ACT treatment stimulated HCASMC proliferation (cell counts) and increased proliferating cell nuclear antigen (PCNA) expression, concomitant with stimulation of Notch signaling as determined by increased Notch receptor (N1, N3) and target gene (HRT 1-3) mRNA levels...
December 6, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29152140/inhibition-of-il-8-mediated-endothelial-adhesion-vsmcs-proliferation-and-migration-by-sirna-tmem98-suggests-tmem98-s-emerging-role-in-atherosclerosis
#2
Guangxin Lv, Hongmei Zhu, Cai Li, Jingyu Wang, Dandan Zhao, Shuyao Li, Le Ma, Guohua Sun, Fang Li, Ying Zhao, Ying Gao
Transmembrane protein 98 (TMEM98), known as a novel gene related to lung cancer, hepatocellular carcinoma, differentiation of T helper 1 cells and normal eye development, has no defined role reported in terms of atherosclerosis (AS). To investigate the potential involvement of TMEM98 during AS processes, its obvious secretion and expression has been initially characterized in hyperlipidemia patients' serum and AS mice's serum respectively. We then explored the possible role of TMEM98 in the pathogenesis of AS in vitro...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29104843/silencing-mir-16-expression-promotes-angiotensin-ii-stimulated-vascular-smooth-muscle-cell-growth
#3
Qingqing Gu, Guannan Zhao, Yinan Wang, Biao Xu, Junming Yue
miRNAs are a class of non-coding endogenous small RNAs that control gene expression at the posttranscriptional level and involved in cell proliferation, migration and differentiation. Dysregulation of miRNA expression is involved in a variety of human diseases including cardiovascular diseases. miRNAs have been shown to regulate vascular smooth muscle cell (VSMC) function and play vital roles in hypertension, restenosis and atherosclerosis. Here we reported that miR-16 as one of miRNAs in the miR-15 family was highly expressed in vascular smooth muscle cells (VSMCs) and involved in angiotensin II (Ang II) mediated VSMC signaling pathways...
March 2017: Cell & Developmental Biology
https://www.readbyqxmd.com/read/29100354/mechanism-of-genistein-regulating-the-differentiation-of-vascular-smooth-muscle-cells-into-osteoblasts-via-the-opg-rankl-pathway
#4
Cheng Shen, Ye Yuan, Fuping Li, Yijie Hu, Yi Song, Shulin Zhao, Qianjin Zhong
Objective: The present study aimed to investigate the mechanism of genistein, a tyrosine kinase inhibitor, regulating the differentiation of vascular smooth muscle cells (VSMCs) into osteoblasts via the OPG/RANKL (Osteoprotegerin/Receptor Activator of Nuclear Factor-κB Ligand) pathway. Methods: The mouse VSMCs were isolated, purified and cultured. We constructed the LV5-Tnfrsf11b overexpression lentiviral vector and LV3-OPG-309 interference lentiviral vector. The OPG overexpression was induced and the growth of VSMCs infected with the lentiviral vector was observed...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29100273/mir-26a-contributes-to-the-pdgf-bb-induced-phenotypic-switch-of-vascular-smooth-muscle-cells-by-suppressing-smad1
#5
Xiaoyan Yang, Mei Dong, Hao Wen, Xiaoling Liu, Meng Zhang, Lianyue Ma, Cheng Zhang, Xiaorong Luan, Huixia Lu, Yun Zhang
The phenotypic switch of vascular smooth muscle cells (VSMCs) is a key event in the pathogenesis of various vascular diseases, such as atherosclerosis and post-angioplasty restenosis. Small non-coding microRNAs (miRNAs) have emerged as critical modulators of VSMC function. In the present study, miR-26a was significantly increased in cultured VSMCs stimulated by platelet-derived growth factor-BB (PDGF-BB) and in arteries with neointimal lesion formation. Moreover, we demonstrated that miR-26a regulates the expression of VSMC differentiation marker genes such as α-smooth muscle actin (α-SMA), calponin and smooth muscle myosin heavy chain (SM-MHC) in PDGF-BB-treated VSMCs...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29089350/unspliced-xbp1-confers-vsmc-homeostasis-and-prevents-aortic-aneurysm-formation-via-foxo4-interaction
#6
Guizhen Zhao, Yi Fu, Zeyu Cai, Fang Yu, Ze Gong, Rongbo Dai, Yanhua Hu, Lingfang Zeng, Qingbo Xu, Wei Kong
Rationale: Although not fully understood, the phenotypic transition of vascular smooth muscle cells exhibits at the early onset of the pathology of aortic aneurysms. Exploring the key regulators that are responsible for maintaining the contractile phenotype of VSMCs may confer vascular homeostasis and prevent aneurysmal disease. X-box binding protein 1, which exists in a transcriptionally inactive unspliced form (XBP1u) and a spliced active form (XBP1s), is a key component in response to endoplasmic reticular (ER) stress...
October 31, 2017: Circulation Research
https://www.readbyqxmd.com/read/29079194/role-of-ros-trpm7-erk1-2-axis-in-high-concentration-glucose-mediated-proliferation-and-phenotype-switching-of-rat-aortic-vascular-smooth-muscle-cells
#7
Meimei Yang, Jing Fang, Qingan Liu, Yan Wang, Zhuobo Zhang
This study investigated the change of transient receptor potential cation channel subfamily M member 7 (TRPM7) expression in rat aortic vascular smooth muscle cells (RAoSMCs) treated with a high concentration of d-glucose (HG) and its role in promoting the proliferative phenotype of RAoSMCs. Chronic exposure to HG increased TRPM7 protein expression and TRPM7 whole-cell currents in RAoSMCs. By contrast, RAoSMC exposure to high concentration of l-glucose and mannital exhibited no such effect. Mechanistically, HG treatment elevated TRPM7 expression by increasing oxidative stress...
October 25, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29021296/lactate-promotes-synthetic-phenotype-in-vascular-smooth-muscle-cells
#8
Libang Yang, Ling Gao, Thomas Nickel, Jing Yang, Jingyi Zhou, Adam Gilbertsen, Zhaohui Geng, Caitlin Johnson, Bernice Young, Craig Henke, Glenn R Gourley, Jianyi Zhang
RATIONALE: The phenotypes of vascular smooth muscle cells (vSMCs) comprise a continuum bounded by predominantly contractile and synthetic cells. Some evidence suggests that contractile vSMCs can assume a more synthetic phenotype in response to ischemic injury, but the mechanisms that activate this phenotypic switch are poorly understood. OBJECTIVE: To determine whether lactate, which increases in response to regional ischemia, may promote the synthetic phenotype in vSMCs...
November 10, 2017: Circulation Research
https://www.readbyqxmd.com/read/28951205/the-protective-role-of-yap1-on-er-stress-induced-cell-death-in-vascular-smooth-muscle-cells
#9
Akira Takaguri, Takashi Kubo, Masaya Mori, Kumi Satoh
Apoptosis of vascular smooth muscle cells (VSMCs) has been implicated in the progression of atherosclerosis, especially in vascular remodelling and plaque rupture. Although it is known that Yes-associated protein 1 (YAP1) is a critical molecule that regulates cell proliferation, differentiation and apoptosis, the role of YAP1 in VSMCs apoptosis remains unknown. In this study, we investigated whether YAP1 modulates VSMC apoptosis induced by endoplasmic reticulum (ER) stress. In cultured VSMC, tunicamycin caused cell death accompanied by an increase in caspase-3 processing and C/EBP homologous protein (CHOP) expression...
November 15, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28946662/ape1-ref-1-inhibits-phosphate-induced-calcification-and-osteoblastic-phenotype-changes-in-vascular-smooth-muscle-cells
#10
Ki Mo Lee, Eun Ok Lee, Yu Ran Lee, Hee Kyoung Joo, Myoung Soo Park, Cuk-Seong Kim, Sunga Choi, Jin-Ok Jeong, Byeong Hwa Jeon
Vascular calcification plays a role in the pathogenesis of atherosclerosis, diabetes, and chronic kidney disease; however, the role of apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) in inorganic phosphate (Pi)-induced vascular smooth muscle cell (VSMC) calcification remains unknown. In this study, we investigated the possible role of APE1/Ref-1 in Pi-induced VSMC calcification. We observed that Pi decreased endogenous APE1/Ref-1 expression and promoter activity in VSMCs, and that adenoviral overexpression of APE1/Ref-1 inhibited Pi-induced calcification in VSMCs and in an ex vivo organ culture of a rat aorta...
September 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28920957/klf4-dependent-perivascular-cell-plasticity-mediates-pre-metastatic-niche-formation-and-metastasis
#11
Meera Murgai, Wei Ju, Matthew Eason, Jessica Kline, Daniel W Beury, Sabina Kaczanowska, Markku M Miettinen, Michael Kruhlak, Haiyan Lei, Jack F Shern, Olga A Cherepanova, Gary K Owens, Rosandra N Kaplan
A deeper understanding of the metastatic process is required for the development of new therapies that improve patient survival. Metastatic tumor cell growth and survival in distant organs is facilitated by the formation of a pre-metastatic niche that is composed of hematopoietic cells, stromal cells and extracellular matrix (ECM). Perivascular cells, including vascular smooth muscle cells (vSMCs) and pericytes, are involved in new vessel formation and in promoting stem cell maintenance and proliferation. Given the well-described plasticity of perivascular cells, we hypothesized that perivascular cells similarly regulate tumor cell fate at metastatic sites...
October 2017: Nature Medicine
https://www.readbyqxmd.com/read/28860223/differentiation-and-application-of-induced-pluripotent-stem-cell-derived-vascular-smooth-muscle-cells
#12
REVIEW
Eithne Margaret Maguire, Qingzhong Xiao, Qingbo Xu
Vascular smooth muscle cells (VSMCs) play a role in the development of vascular disease, for example, neointimal formation, arterial aneurysm, and Marfan syndrome caused by genetic mutations in VSMCs, but little is known about the mechanisms of the disease process. Advances in induced pluripotent stem cell technology have now made it possible to derive VSMCs from several different somatic cells using a selection of protocols. As such, researchers have set out to delineate key signaling processes involved in triggering VSMC gene expression to grasp the extent of gene regulatory networks involved in phenotype commitment...
November 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28843066/deriving-vascular-smooth-muscle-cells-from-mesenchymal-stromal-cells-evolving-differentiation-strategies-and-current-understanding-of-their-mechanisms
#13
REVIEW
Xiaoqing Zhang, Michelle P Bendeck, Craig A Simmons, J Paul Santerre
Vascular smooth muscle cells (VSMCs) play essential roles in regulating blood vessel form and function. Regeneration of functional vascular smooth muscle tissue to repair vascular diseases is an area of intense research in tissue engineering and regenerative medicine. For functional vascular smooth muscle tissue regeneration to become a practical therapy over the next decade, the field will need to have access to VSMC sources that are effective, robust and safe. While pluripotent stem cells hold good future promise to this end, more immediate translation is expected to come from approaches that generate functional VSMCs from adult sources of multipotent adipose-derived and bone marrow-derived mesenchymal stromal cells (ASCs and BMSCs)...
August 15, 2017: Biomaterials
https://www.readbyqxmd.com/read/28829997/msx2-is-required-for-vascular-smooth-muscle-cells-osteoblastic-differentiation-but-not-calcification-in-insulin-resistant-ob-ob-mice
#14
Maria Claudina Andrade, Luciana S Carmo, Elisangela Farias-Silva, Marcel Liberman
BACKGROUND AND AIMS: Obesity and diabetes potentiate vascular calcification by increasing vascular smooth muscle cells osteoblastic differentiation mediated by the transcription factor Msx2 and bone morphogenetic protein-2 signaling. However, Bmp-2/Msx2 crosstalk to induce VSMC osteogenic phenotype transition and calcification is poorly understood in diabetes. We aimed to investigate mechanisms underlying Bmp-2-driven VSMC osteogenic differentiation and calcification in leptin-deficient ob/ob mice...
July 29, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28816361/msel-1l-deficiency-affects-vasculogenesis-and-neural-stem-cell-lineage-commitment
#15
Marina Cardano, Giuseppe R Diaferia, Luciano Conti, Simona Baronchelli, Alessandro Sessa, Vania Broccoli, Andrea Barbieri, Pasquale De Blasio, Ida Biunno
mSEL-1L is a highly conserved ER-resident type I protein, involved in the degradation of misfolded peptides through the ubiquitin-proteasome system (UPS), a pathway known to control the plasticity of the vascular smooth muscle cells (VSMC) phenotype and survival. In this article, we demonstrate that mSEL-1L deficiency interferes with the murine embryonic vascular network, showing particular irregularities in the intracranic and intersomitic neurovascular units and in the cerebral capillary microcirculation...
August 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28713961/adipose-differentiation%C3%A2-related-protein-knockdown-inhibits-vascular-smooth-muscle-cell-proliferation-and-migration-and-attenuates-neointima-formation
#16
Haomin Zhao, Tao Han, Xin Hong, Dajun Sun
Vascular smooth muscle cells (VSMCs) have an important role in atherosclerosis development. Evidence has demonstrated that adipose differentiation‑related protein (ADRP) is associated with foam cell formation and atherosclerosis progression. However, to the best of our knowledge, no previous studies have investigated the role of ADRP knockdown in platelet‑derived growth factor (PDGF)‑stimulated proliferation and migration of VSMCs in vitro. Furthermore, the effect of ADRP knockdown on neointima formation in vivo remains unclear...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28659610/upregulation-of-arylsulfatase-b-in-carotid-atherosclerosis-is-associated-with-symptoms-of-cerebral-embolization
#17
Erik Biros, Corey S Moran, Jane Maguire, Elizabeth Holliday, Christopher Levi, Jonathan Golledge
The aim of this study was to identify genes for which the expression within carotid atherosclerosis was reproducibly associated with the symptoms of cerebral embolization. Two publically available microarray datasets E-MEXP-2257 and GSE21545 were analysed using GeneSpring 11.5. The two datasets utilized a total of 22 and 126 carotid atherosclerosis samples, obtained from patients with and without symptoms of cerebral embolization, respectively. To assess whether the findings were reproducible we analysed carotid atherosclerosis samples from another 8 patients with and 7 patients without symptoms of cerebral embolization using real-time PCR...
June 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28659168/gene-expression-profiles-and-signaling-mechanisms-in-%C3%AE-2b-adrenoceptor-evoked-proliferation-of-vascular-smooth-muscle-cells
#18
Anna Huhtinen, Vesa Hongisto, Asta Laiho, Eliisa Löyttyniemi, Dirk Pijnenburg, Mika Scheinin
BACKGROUND: α2-adrenoceptors are important regulators of vascular tone and blood pressure. Regulation of cell proliferation is a less well investigated consequence of α2-adrenoceptor activation. We have previously shown that α2B-adrenoceptor activation stimulates proliferation of vascular smooth muscle cells (VSMCs). This may be important for blood vessel development and plasticity and for the pathology and therapeutics of cardiovascular disorders. The underlying cellular mechanisms have remained mostly unknown...
June 28, 2017: BMC Systems Biology
https://www.readbyqxmd.com/read/28655510/collagen-xiv-and-a-related-recombinant-fragment-protect-human-vascular-smooth-muscle-cells-from-calcium-phosphate-induced-osteochondrocytic-transdifferentiation
#19
Christian Freise, Veronika Bobb, Uwe Querfeld
Transdifferentiation of vascular smooth muscle cells (VSMC) promotes the development of vascular calcifications such as arteriosclerosis. The aim was to investigate effects of specific extracellular matrix (ECM) components on transdifferentiation of VSMC to identify novel ECM-based therapeutic tools. Human collagens I & IV (CI, CIV) along with collagen XIV (CXIV) and a CXIV-derived fragment (CXIV-F), both of which induce differentiation, were applied in an in-vitro model of calcium-/phosphate (Ca/P)-induced osteochondrocytic transdifferentiation of human and murine VSMC...
September 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28644441/micrornas-143-and-145-induce-epithelial-to-mesenchymal-transition-and-modulate-the-expression-of-junction-proteins
#20
Lidia Avalle, Danny Incarnato, Aurora Savino, Marta Gai, Francesca Marino, Sara Pensa, Isaia Barbieri, Michael B Stadler, Paolo Provero, Salvatore Oliviero, Valeria Poli
Transforming growth factor (TGF)-β is one of the major inducers of epithelial to mesenchymal transition (EMT), a crucial program that has a critical role in promoting carcinoma's metastasis formation. MicroRNAs-143 and -145, which are both TGF-β direct transcriptional targets, are essential for the differentiation of vascular smooth muscle cells (VSMC) during embryogenesis, a TGF-β-dependent process reminiscent of EMT. Their role in adult tissues is however less well defined and even ambiguous, as their expression was correlated both positively and negatively with tumor progression...
October 2017: Cell Death and Differentiation
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