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https://www.readbyqxmd.com/read/27531958/single-cell-rnaseq-reveals-cell-adhesion-molecule-profiles-in-electrophysiologically-defined-neurons
#1
Csaba Földy, Spyros Darmanis, Jason Aoto, Robert C Malenka, Stephen R Quake, Thomas C Südhof
In brain, signaling mediated by cell adhesion molecules defines the identity and functional properties of synapses. The specificity of presynaptic and postsynaptic interactions that is presumably mediated by cell adhesion molecules suggests that there exists a logic that could explain neuronal connectivity at the molecular level. Despite its importance, however, the nature of such logic is poorly understood, and even basic parameters, such as the number, identity, and single-cell expression profiles of candidate synaptic cell adhesion molecules, are not known...
August 30, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27342097/prevalence-of-bovine-viral-diarrhoea-virus-in-cattle-farms-in-hungary
#2
Ágnes Szabára, Zsolt Lang, József Földi, Ákos Hornyák, Tamás Abonyi, László Ózsvári
A study was performed to survey the virological prevalence of bovine viral diarrhoea (BVD) virus (BVDV) in cattle herds in Hungary between 2008 and 2012. A total of 40,413 samples for BVDV detection and 24,547 samples for antibody testing were collected from 3,247 herds (570,524 animals), thus representing approximately 75% of the cattle population in Hungary. Retrospective Bayesian analysis demonstrated that (1) the herd-level true virus prevalence was 12.4%, (2) the mean individual (within-herd) true virus prevalence was 7...
June 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27013987/facilitating-mitochondrial-calcium-uptake-improves-activation-induced-cerebral-blood-flow-and-behavior-after-mtbi
#3
Madhuvika Murugan, Vijayalakshmi Santhakumar, Sridhar S Kannurpatti
Mild to moderate traumatic brain injury (mTBI) leads to secondary neuronal loss via excitotoxic mechanisms, including mitochondrial Ca(2+) overload. However, in the surviving cellular population, mitochondrial Ca(2+) influx, and oxidative metabolism are diminished leading to suboptimal neuronal circuit activity and poor prognosis. Hence we tested the impact of boosting neuronal electrical activity and oxidative metabolism by facilitating mitochondrial Ca(2+) uptake in a rat model of mTBI. In developing rats (P25-P26) sustaining an mTBI, we demonstrate post-traumatic changes in cerebral blood flow (CBF) in the sensorimotor cortex in response to whisker stimulation compared to sham using functional Laser Doppler Imaging (fLDI) at adulthood (P67-P73)...
2016: Frontiers in Systems Neuroscience
https://www.readbyqxmd.com/read/26479624/analysis-of-focused-laser-differential-interferometry
#4
B E Schmidt, J E Shepherd
A computational method for predicting the output of a focused laser differential interferometer (FLDI) given an arbitrary density field is presented. The method is verified against analytical predictions and experimental data. The FLDI simulation software is applied to the problem of measuring Mack-mode wave packets in a hypervelocity boundary layer on a 5° half-angle cone. The software is shown to complement experiments by providing the necessary information to allow quantitative density fluctuation magnitudes to be extracted from experimental measurements...
October 1, 2015: Applied Optics
https://www.readbyqxmd.com/read/21911212/frequency-tuning-in-the-rat-whisker-barrel-cortex-revealed-through-rbc-flux-maps
#5
Sridhar S Kannurpatti, Bharat B Biswal
The rodent whisker barrel cortex is ideal for studies related to sensory processing and neural plasticity in the brain. However, its small spatial dimensions challenge optical and other imaging technologies mapping cortical hemodynamics as functional resolution (the ability to spatially and selectively discriminate signals from microvascular compartments) limit measurement accuracy. To precisely map hemodynamic activity within the rat posteriomedial barrel subfield (PMBSF), we used functional Laser Doppler Imaging (fLDI) at high spatial resolution with optimized detection and analysis...
October 12, 2011: Brain Research
https://www.readbyqxmd.com/read/11967068/molecular-characterization-of-phenyllactate-dehydratase-and-its-initiator-from-clostridium-sporogenes
#6
Sandra Dickert, Antonio J Pierik, Wolfgang Buckel
The heterotrimeric phenyllactate dehydratase from Clostridium sporogenes, FldABC, catalyses the reversible dehydration of (R)-phenyllactate to (E)-cinnamate in two steps: (i) CoA-transfer from the cofactor cinnamoyl-CoA to phenyllactate to yield phenyllactyl-CoA and the product cinnamate mediated by FldA, a (R)-phenyllactate CoA-transferase; followed by (ii) dehydration of phenyllactyl-CoA to cinnamoyl-CoA mediated by heterodimeric FldBC, a phenyllactyl-CoA dehydratase. Phenyllactate dehydratase requires initiation by ATP, MgCl2 and a reducing agent such as dithionite mediated by an extremely oxygen-sensitive initiator protein (FldI) present in the cell-free extract...
April 2002: Molecular Microbiology
https://www.readbyqxmd.com/read/11766961/fluorene-degradation-by-sphingomonas-sp-lb126-proceeds-through-protocatechuic-acid-a-genetic-analysis
#7
P Wattiau, L Bastiaens, R van Herwijnen, L Daal, J R Parsons, M E Renard, D Springael, G R Cornelis
Sphingomonas sp. LB126 is able to utilize fluorene as sole source of carbon and energy. In the present study, a mutagenic vector was constructed and a "plasmid rescue" strategy was set up to isolate a 16.5-kb DNA fragment containing genes required for fluorene degradation. A 14.5-kb portion of the cloned DNA was sequenced revealing thirteen open reading frames. Two encoded hypothetical proteins (FldE and FldY) similar to transcriptional regulators and one (ORF360) located on an IS-like element (ISSsp126) encoded a putative transposase...
December 2001: Research in Microbiology
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