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https://www.readbyqxmd.com/read/29205609/effect-of-tamoxifen-on-fibrosis-collagen-content-and-transforming-growth-factor-%C3%AE-1-%C3%AE-2-and-%C3%AE-3-expression-in-common-bile-duct-anastomosis-of-pigs
#1
Orlando Hiroshi Kiono Siqueira, Karen Jesus Oliveira, Angela Cristina Gouvêa Carvalho, Antonio Claudio Lucas da Nóbrega, Renata Frauches Medeiros, Bruno Felix-Patrício, Fábio Otero Áscoli, Beni Olej
End-to-end anastomosis in the treatment for bile duct injury during laparoscopic cholecystectomy has been associated with stricture formation. The aim of this study was to experimentally investigate the effect of oral tamoxifen (tmx) treatment on fibrosis, collagen content and transforming growth factor-β1, -β2 and -β3 expression in common bile duct anastomosis of pigs. Twenty-six pigs were divided into three groups [sham (n = 8), control (n = 9) and tmx (n = 9)]. The common bile ducts were transected and anastomosed in the control and tmx groups...
December 4, 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/29201224/expression-of-nf-e2-related-factor-2-in-a-rat-dural-arteriovenous-fistula-model
#2
Limin Dou, Wenhua Yu
Intracranial dural arteriovenous fistulas (DAVFs) are complex intracranial vascular malformations that may lead to hemorrhage. Although the precise mechanisms by which DAVFs occur remain unknown, dural angiogenesis may be a vital factor in its pathogenesis. Nuclear factor erythroid 2-related factor 2 (Nrf2) significantly influences angiogenesis; however, the association between DAVF and Nrf2 remains unclear. Therefore, the present study investigated whether DAVF alters the expression of Nrf2 in an experimental animal model of DAVF...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29199083/factors-correlating-with-microsurgical-performance-a-clinical-and-experimental-study
#3
Mark V Schaverien, Jun Liu, Charles E Butler, Jesse C Selber
BACKGROUND: Microsurgery is one the most complex surgical skills to master. The factors correlating with microsurgical performance, however, are poorly understood. Understanding these factors will aid in the training and assessment of microsurgeons. METHODS: A total of 29 microsurgery fellows enrolled in a dedicated 1-year microsurgery fellowship were included in the study. For the clinical evaluations, microsurgical anastomosis performance was evaluated during multiple procedures in the operating room at the start, midpoint, and end of the fellowship by all departmental faculty using a validated microsurgical assessment tool...
November 30, 2017: Journal of Surgical Education
https://www.readbyqxmd.com/read/29198926/does-the-application-of-ankaferd-blood-stopper-rectally-have-positive-effects-on-the-healing-of-colorectal-anastomosis-and-prevention-of-anastomotic-leakage-an-experimental-study
#4
Serdar Kuru, Kemal Kismet, Yusuf Murat Bag, Aziz Mutlu Barlas, Mehmet Senes, Murat Durak, Nihat Yumusak, Mustafa Kemal Urhan, Turgut Cavusoglu, Recep Pekcici
The purpose of this experimental study was to evaluate the potential effects on the healing of colorectal anastomoses of the rectal administration of Ankaferd Blood Stopper (ABS). Thirty Wistar-Albino male rats were randomly separated into 3 groups. In the sham group, only laparotomy and colonic mobilization was performed. In the other 2 groups, colon transection and anastomosis were carried out. Saline (2 mL, 0.9% NaCl) was given rectally via a feeding tube for 10 days after the surgical procedure in the sham and control groups...
November 29, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29146107/cardiovascular-anatomy-in-children-with-bidirectional-glenn-anastomosis-regarding-the-transcatheter-fontan-completion
#5
Aleksander Sizarov, Francesca Raimondi, Damien Bonnet, Younes Boudjemline
BACKGROUND: Transcatheter stent-secured completion of total cavopulmonary connection (TCPC) after surgical preparations during the Glenn anastomosis procedure has been reported, but complications from this approach have precluded its clinical acceptance. AIMS: To analyse cardiovascular morphology and dimensions in children with bidirectional Glenn anastomosis, regarding the optimal device design for transcatheter Fontan completion without special surgical "preconditionings"...
November 13, 2017: Archives of Cardiovascular Diseases
https://www.readbyqxmd.com/read/29133168/autologous-granulation-tissue-tubes-for-replacement-of-urethral-defects-an-experimental-study-in-male-rabbits
#6
Shi-Wei Jiang, Zhong-Hua Xu, Yuan-Yuan Zhao, Lei Yan, Zun-Lin Zhou, Gang-Li Gu
INTRODUCTION: Tubularized urethroplasty is commonly performed in clinical practice using genital skin flaps, bladder mucosa, and buccal mucosa. However, the long-term effects are not satisfying, and donor site morbidities remain a problem. Besides, those grafts are unavailable with malignant conditions of the urinary tract, a history of lichen sclerosis, or oral disease. OBJECTIVE: An autologous granulation tissue tube of any required length and diameter can be produced by implanting foreign objects subcutaneously (Summary Fig...
October 31, 2017: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/29129243/enteral-feeding-with-low-methoxyl-pectin-accelerates-colonic-anastomosis-healing-in-rats
#7
Fumiyo Yamada, Naoyuki Endo, Sho Miyatake, Goro Ebisu, Kazuo Hino
OBJECTIVE: Enteral feeding with pectin has proven beneficial for anastomosis healing in rats. The aim of this study was to investigate the effects of low-methoxyl pectin (LMP) or high-methoxyl pectin (HMP), on colonic anastomosis healing in rats. METHODS: Male Sprague-Dawley rats (age 7 wk) were fed liquid diets containing LMP, HMP, or no pectin (pectin-free [PF]) for 14 d (n = 10/group). The rats underwent colonic anastomosis surgery on day 7 and were sacrificed on day 14...
January 2018: Nutrition
https://www.readbyqxmd.com/read/29117903/histopathological-and-immunohistochemical-analysis-of-the-use-of-collagen-dressing-as-a-reinforcement-of-esophagic-anastomosis-in-a-rat-experimental-model
#8
Rocio Garcia-Perez, Vicente Munitiz, Carlos Manuel Martinez-Caceres, David Ruiz de Angulo, Angeles Ortiz, Luisa F Martinez de Haro, Diana Navas, Pascual Parrilla
INTRODUCTION: One of the most severe complications after esophaguectomy is anastomotic dehiscence. The use of collagen sponges could be an effective way to resolve this complication. Our objective was to perform an experimental model of esophageal anastomosis in rats to study these mechanisms. METHODS: A total of 50 Sprague-Dawley rats were used divided into 2 groups, Tachosil(®) group (n=25) and control group (n=25). After the section of the abdominal esophagus a single-layer esophago-gastric anastomosis was performed reinforced with 1cm of Tachosil(®) wrapping the anastomosis in group 1...
November 5, 2017: Cirugía Española
https://www.readbyqxmd.com/read/29089696/the-effects-of-simvastatin-on-ischemia-reperfusion-injury-in-an-experimental-colon-anastomosis-model
#9
Mahmut Akarsu, Oral Saygun, Kuzey Aydinuraz, Oktay Aydin, Cagatay Erden Daphan, Fatma Benli Tanrıkulu, Ucler Kisa, Faruk Metin Comu
Anastomotic leakage is more frequently reported in colonic anastomoses. Ischemia reperfusion injury is one of the main reasons for anastomotic leakage. Simvastatin is known to prevent tissue damage induced by free oxygen radicals after ischemia reperfusion injury. The effect of simvastatin on colonic anastomosis impaired by ischemia reperfusion injury is investigated. Single layer, end-to-end colocolic anastomosis after 0.5-cm colon resection was performed in Wistar Albino rats. In Group 1 (control) (n = 10), colonic anastomosis without I-R was performed...
October 2017: Indian Journal of Surgery
https://www.readbyqxmd.com/read/29037059/-experimental-study-on-the-resistance-to-intraluminal-pressure-between-handsewn-and-stapled-anastomosis-in-vitro-experimental-study
#10
Marius Florin Coros, Rares Georgescu, Zalán Benedek, Sorin Sorlea, Ghenadie Pascarenco, Cornelia Mesaros, Orsolya Hankó-Bauer
Intoduction and aim: The aim of the study was to compare the resistance to pressure of stapled and manually handsewn intestinal suture lines on in vitro pig intestine model. METHOD: We performed different types of stapled and manual sutures and the pressure level was measured using a differential pressure manometer. RESULTS: Although the hand-sewn end-to-end suture turned out to be the most resistant to pressure, statistical analysis revealed no significant differences compared to stapled suture (p = 0...
October 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/28994804/biodegradable-magnesium-stent-treatment-of-saccular-aneurysms-in-a-rat-model-introduction-of-the-surgical-technique
#11
Edin Nevzati, Jeannine Rey, Daniel Coluccia, Donato D'Alonzo, Basil Grüter, Luca Remonda, Javier Fandino, Serge Marbacher
The steady progess in the armamentarium of techniques available for endovascular treatment of intracranial aneurysms requires affordable and reproducable experimental animal models to test novel embolization materials such as stents and flow diverters. The aim of the present project was to design a safe, fast, and standardized surgical technique for stent assisted embolization of saccular aneurysms in a rat animal model. Saccular aneurysms were created from an arterial graft from the descending aorta.The aneurysms were microsurgically transplanted through end-to-side anastomosis to the infrarenal abdominal aorta of a syngenic male Wistar rat weighing >500 g...
October 1, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28984618/reduced-th-expression-and-%C3%AE-synuclein-accumulation-contribute-towards-nigrostriatal-dysfunction-in-experimental-hepatic-encephalopathy
#12
Isabel Suárez, Guillermo Bodega, Miguel Rubio, Benjamín Fernández
PURPOSE: The present work examines α-synuclein expression in the nigrostriatal system of a rat chronic hepatic encephalopathy model induced by portacaval anastomosis (PCA). There is evidence that dopaminergic dysfunction in disease conditions is strongly associated with such expression. Possible relationships among dopaminergic neurons, astroglial cells and α-synuclein expression were sought. METHODS: Brain tissue samples from rats at 1 and 6 months post-PCA, and controls, were analysed immunohistochemically using antibodies against tyrosine hydroxylase (TH), α-synuclein, glial fibrillary acidic protein (GFAP) and ubiquitin (Ub)...
2017: Restorative Neurology and Neuroscience
https://www.readbyqxmd.com/read/28952820/a-novel-laparoscopic-surgery-model-with-a-device-to-expand-the-abdominal-working-space-in-rats-the-influence-of-pneumoperitoneum-and-skin-incision-length-on-postoperative-inflammatory-cytokines
#13
Naruhiko Murase, Takahisa Tainaka, Hiroo Uchida, Akinari Hinoki, Chiyoe Shirota, Kazuki Yokota, Kazuo Oshima, Ryo Shirotsuki, Kosuke Chiba, Yujiro Tanaka
PURPOSE: Experimental models of laparoscopic surgery generally use large animals owing to a sufficient abdominal working space. We developed a novel laparoscopic surgery model in rats. We performed intestinal anastomosis to demonstrate the feasibility and reliability of this model. MATERIALS AND METHODS: We designed a device for rats that expanded the abdominal working space and allowed us to manipulate the intraperitoneal organs by hand under direct vision with pneumoperitoneum...
September 27, 2017: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
https://www.readbyqxmd.com/read/28923652/orthotopic-kidney-transplantation-in-the-rat-with-the-use-of-a-sleeve-arterial-anastomosis-method-and-a-modified-stenting-technique-for-renal-veins
#14
Z-D Jin, L-N Xue, L-S Peng
BACKGROUND: We describe a simple and reliable orthotopic kidney transplantation method in rats with the use of sleeve arterial anastomosis and a modified stenting technique for anastomosis of the renal vein (RV). METHODS: Male Fischer and Lewis rats were used as kidney donors and recipients, respectively, and their left kidneys were harvested in situ. In the control rats (n = 30), the renal artery (RA) and RV anastomoses were performed end-to-end with interrupted sutures by means of the conventional technique...
October 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28902940/bacterial-translocation-and-mortality-on-rat-model-of-intestinal-ischemia-and-obstruction
#15
Rafael Izar Domingues da Costa, Roberto Rasslan, Marcia Kiyomi Koike, Edivaldo Massazo Utiyama, Edna Frasson de Souza Montero
Purpose: To develop an experimental model of intestinal ischemia and obstruction followed by surgical resection of the damaged segment and reestablishment of intestinal transit, looking at bacterial translocation and survival. Methods: After anesthesia, Wistar rats was subject to laparotomy, intestinal ischemia and obstruction through an ileal ligature 1.5cm of ileum cecal valve; and the mesenteric vessels that irrigate upstream of the obstruction site to approximately 7 to 10 cm were ligated...
August 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28894657/optimization-of-an-arterialized-venous-fasciocutaneous-flap-in-the-abdomen-of-the-rat
#16
Diogo Casal, Eduarda Mota-Silva, Diogo Pais, Inês Iria, Paula A Videira, David Tanganho, Sara Alves, Luís Mascarenhas-Lemos, José Martins Ferreira, Mário Ferraz-Oliveira, Valentina Vassilenko, João Goyri O'Neill
BACKGROUND: Although numerous experimental models of arterialized venous flaps (AVFs) have been proposed, no single model has gained widespread acceptance. The main aim of this work was to evaluate the survival area of AVFs produced with different vascular constructs in the abdomen of the rat. METHODS: Fifty-three male rats were divided into 4 groups. In group I (n = 12), a 5-cm-long and 3-cm-wide conventional epigastric flap was raised on the left side of the abdomen...
August 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/28883693/effects-of-albumin-glutaraldehyde-glue-on-healing-of-colonic-anastomosis-in-rats
#17
Kalliopi Despoudi, Ioannis Mantzoros, Orestis Ioannidis, Aggeliki Cheva, Nikolaos Antoniou, Dimitrios Konstantaras, Savvas Symeonidis, Manousos George Pramateftakis, Efstathios Kotidis, Stamatis Angelopoulos, Konstantinos Tsalis
AIM: To evaluate the effect of local surgical adhesive glue (albumin/glutaraldehyde-Bioglue) on the healing of colonic anastomoses in rats. METHODS: Forty Albino-Wistar male rats were randomly divided into two groups, with two subgroups of ten animals each. In the control group, an end-to-end colonic anastomosis was performed after segmental resection. In the Bioglue group, the anastomosis was protected with extraluminar application of adhesive glue containing albumin and glutaraldehyde...
August 21, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28880048/histopathological-changes-associated-to-an-absorbable-fibrin-patch-tachosil%C3%A2-covering-in-an-experimental-model-of-high-risk-colonic-anastomoses
#18
C García-Vásquez, S Gómez García de Las Heras, C Pastor Idoate, D De Pablo, M J Fernández-Aceñero
BACKGROUND: TachoSil® is a fibrin sponge that contains fibrinogen and thrombin and is a useful adjuvant to enhance control of air leaks in thoracic surgery and to control bleeding in vascular and general surgery. Its use in intestinal surgery to prevent suture dehiscence is currently under investigation. MATERIAL AND METHODS: We report the results of a prospective randomized experimental study on 33 large white pigs in which a high-risk suture was created by induction of ischemia...
September 7, 2017: Histology and Histopathology
https://www.readbyqxmd.com/read/28867319/transfer-of-learning-from-practicing-microvascular-anastomosis-on-silastic-tubes-to-rat-s-abdominal-aorta
#19
Pooneh Mokhtari, Ali Tayebi Meybodi, Michael T Lawton, Andre Payman, Arnau Benet
OBJECT: Learning to perform microvascular anastomosis is difficult. Laboratory practice models using artificial vessels are frequently used for this purpose. However, the efficacy of such practice models has not been objectively assessed on the performance of microvascular anastomosis during live surgical settings. This study was carried out to assess the transfer of learning from practicing microvascular anastomosis on tubes to anastomosing rat's abdominal aorta. METHODS: Ten surgeons without any experience in microvascular anastomosis were randomly assigned to an experimental or a control group...
August 31, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28854521/substitution-rate-based-screening-model-to-assess-stenosis-progression-in-experimental-stenotic-arteriovenous-grafts
#20
Chung-Dann Kan, Wei-Ling Chen, Chia-Hung Lin, Ming-Jui Wu, Yi-Chen Mai
An arteriovenous graft (AVG) has a higher patency rate in stenosis progression at the venous anastomosis site, which causes coexisting inflow and outflow stenoses. This leads to increases in blood pressure, flow velocity, and flow resistance, resulting in hemodialysis (HD) vascular access dysfunction from early clots and thrombosis to the progression of coexisting stenoses. To prevent vascular access complications such as inflow or outflow stenoses, this study proposes a novel examination method in an experimental AVG system using a substitution-rate based screening model...
October 23, 2017: Technology and Health Care: Official Journal of the European Society for Engineering and Medicine
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