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https://www.readbyqxmd.com/read/29475213/modified-multivisceral-transplantation-with-native-spleen-removal-in-rats
#1
Pablo Stringa, Nidia Monserrat Arreola, Ane M Andres Moreno, Carlota Largo, Martín Rumbo, Francisco Hernandez
BACKGROUND:  Modified multivisceral transplantation (MMVTx) refers to the use of a graft that includes all abdominal organs except the liver. The use of this type of transplant in children and adults expanded over the last years with good results. However, long-term survival in experimental models has not been reported. Our aim is to describe in detail some technical modifications of MMVTx to obtain long-term survival. MATERIALS AND METHODS:  Syngeneic (Lewis-Lewis) heterotopic MMVTx was performed in 16 male rats (180-250 g)...
February 23, 2018: European Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29473452/a-new-surgical-device-for-anterograde-intraoperative-rectal-washout
#2
Fabio Rondelli, Roberto Santinelli, Paolo Stella, Walter Bugiantella, Graziano Ceccarelli, Ruben Carlo Balzarotti, Michele De Rosa, Nicola Avenia
BACKGROUND: Colorectal cancer is the fourth most diffuse cause of death in the world and local recurrence is associated with a reduced long-term life expectancy, with a reduced quality of life. Rectal washout at the anastomosis site leads to a statistically significant reduction of local recurrences. METHODS: We developed the idea of a new laparoscopic stapler with an integrated washout system that could decontaminate the rectal stump before resection, without the need to enlarge the standard surgical incision or even to distort the incision site, closing the rectal stump just below the inferior part of the cancer, and then proceeding with the resection and stapling of the distal part of the tumor...
February 1, 2018: Surgical Innovation
https://www.readbyqxmd.com/read/29454630/microsurgical-anastomosis-of-renal-vasculature-in-rats-a-practical-platform-for-acellular-kidney-transplantation
#3
Abdol-Mohammad Kajbafzadeh, Reza Khorramirouz, Seyede Maryam Kameli, Behnam Nabavizadeh
End-stage renal disease is becoming a contemporary global concern with increasing prevalence. The available treatment strategies are limited to dialysis and renal transplantation. However, limited organ supply and autoimmune rejection are the shortcomings that limit widespread application of transplantation. Favorably, regenerative medicine is able to provide acellular natural scaffolds for renal transplantation. Experimental surgeries in animal models are a fundamental step in transplantation research. This video presents a practical method for transplantation of bilateral acellular kidneys in a rat model, which could serve as a key step for further research...
February 3, 2018: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/29439318/the-effect-of-three-different-surgical-techniques-for-colon-anastomosis-on-regional-postoperative-microperfusion-laser-doppler-flowmetry-study-in-pigs
#4
Milan Kaska, Slavomir Blazej, Zdenek Turek, Ales Ryska, Boris Jegorov, Vera Radochova, Jan Bezouska, Jiri Paral
BACKGROUND: The optimal surgical approach to reconnecting bowel ends safely after resection is of great importance. OBJECTIVES: This project is focused on assessment of the perianastomotic microcirculation quality in the short postoperative period when using three different anastomosis techniques in experimental animal. METHODS: The experimental study involved 27 young female domestic pigs divided into three subgroups of 9 animals according to each surgical method of anastomosis construction in the sigmoid colon region: by manual suture, by stapler, or by gluing...
2018: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/29436277/initial-load-stability-of-different-trachea-suture-techniques-tests-on-an-ex-vivo-model
#5
Andreas Kirschbaum, Helen Abing, Nikolas Mirow
Objective Tracheal anastomosis can be performed with different suture techniques. In this experimental work, the resilience of anastomotic techniques to pressure and tensile stress was studied. Study Design Ex vivo pig model. Setting Experimental. Subjects and Methods The trachea with the 2 main bronchi in freshly slaughtered pigs was isolated and intubated (CH 8.0). Both main bronchi were closed distally by a stapler. After resection of the trachea, an anastomosis (n = 15 per group) was created: group 1, single interrupted sutures; group 2, continuous running suture; group 3, mixed technique...
February 1, 2018: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/29434447/enteral-nutrition-combined-with-glutamine-promotes-recovery-after-ileal-pouch-anal-anastomosis-in-rats
#6
Yan-Yan Xu, An-Qi He, Gang Liu, Kai-Yu Li, Jian Liu, Tong Liu
AIM: To assess the effect of enteral nutrition (EN) supplemented with glutamine on recovery after ileal pouch-anal anastomosis (IPAA) in rats, to provide an experimental basis for nutritional support in patients with ulcerative colitis (UC) after IPAA. METHODS: Male Sprague-Dawley (SD) rats were randomly divided into three groups ( n = 8) after IPAA operation using a microsurgical technique. From the third postoperative day, rats in the control group, EN group, and immune nutrition (IN) group were fed standard rat chow, short peptide EN, and short peptide EN combined with glutamine ad libitum , respectively...
February 7, 2018: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29433870/parecoxib-s-effects-on-anastomotic-and-abdominal-wound-healing-a-randomized-controlled-trial
#7
Eirini Martinou, Stamatoula Drakopoulou, Eftychia Aravidou, Theodore Sergentanis, Agathi Kondi-Pafiti, Eriphyli Argyra, Dionysios Voros, Georgios P Fragulidis
BACKGROUND: Current evidence regarding the effects of selective cyclooxygenase inhibitors on gastrointestinal anastomoses is controversial. An experimental randomized control study was conducted in our institution to histopathologically evaluate the consequences of parecoxib, on intestinal and abdominal wound healing. METHODS: Twenty-four adult Wistar rats underwent laparotomy, ascending colon transection, and hand-sewn anastomosis. They were randomized to receive either parecoxib (0...
March 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29424651/novel-experimental-surgical-strategy-to-prevent-traumatic-neuroma-formation-by-combining-a-3d-printed-y-tube-with-an-autograft
#8
Anne Bolleboom, Godard C W de Ruiter, J Henk Coert, Bastiaan Tuk, Jan C Holstege, Johan W van Neck
OBJECTIVE Traumatic neuromas may develop after nerve injury at the proximal nerve stump, which can lead to neuropathic pain. These neuromas are often resistant to therapy, and excision of the neuroma frequently leads to recurrence. In this study, the authors present a novel surgical strategy to prevent neuroma formation based on the principle of centro-central anastomosis (CCA), but rather than directly connecting the nerve ends to an autograft, they created a loop using a 3D-printed polyethylene Y-shaped conduit with an autograft in the distal outlets...
February 9, 2018: Journal of Neurosurgery
https://www.readbyqxmd.com/read/29410339/comparison-of-four-different-methods-for-direct-hypoglossal-facial-nerve-anastomosis-in-rats
#9
Pengfei Liu, Chenlong Liao, Wenxiang Zhong, Min Yang, Pengyang Li, Wenchuan Zhang
BACKGROUND: Classic hypoglossal-facial nerve anastomosis inevitably causes hemitongue atrophy and dysfunction. Thus, many variants have been developed to reduce tongue-related morbidities. A comparative study concerning these techniques was conducted in rats to systematically evaluate long-term functional and histological outcomes of the recipient and donor systems. METHODS: Rats (8 per group) were treated by end-to-end neurorrhaphy (EEN), EEN using the hemi-sectioned and longitudinally splitted donor nerve (EEN-Hemi), and end-to-side neurorrhaphy through a perineurial window (ESN-PW) or 30-40% partial neurotomy (ESN-PN)...
February 1, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29386762/antireflux-status-post-roux-en-y-anastomosis-an-experimental-study-for-optimal-antireflux-technique
#10
Ajay Kumar Verma, Om Prakash Purbey, Shiv Narain Kureel, Archika Gupta, Anand Pandey, Kanoujia Sunil, Digamber Chaubey
Background: Roux-en-Y hepaticojejunostomy has been a gold standard to establish biliary-enteric anastomosis for various surgical indications, but associated with variable incidences of cholangitis. This experimental study was conducted to report a modification in Roux-en-Y anastomosis for possible better alternative to provide antireflux procedure after Roux-en-Y biliary-enteric anastomosis with the aim to minimize the possibility of reflux and its consequences. Materials and Methods: For experimental study, the required fresh segment of Lamb's small intestine was procured...
January 2018: Journal of Indian Association of Pediatric Surgeons
https://www.readbyqxmd.com/read/29376595/-experimental-work-reconstruction-of-the-pelvi-ureteric-junction-and-ureter-using-testicular-tunica-vaginalis-autograft
#11
A Ch Usupbaev, R M Kurbanaliev, G S Chernetsova, I V Kolesnichenko, B M Sultanov, N M Myrzakanov, A O Zolotukhin, N A Vagner
AIM: To evaluate the results of surgical reconstruction of the upper urinary tract using an autograft of testicular tunica vaginalis in experimental animals. MATERIALS AND METHODS: The article presents the results of partial replacement of the renal pelvis and ureter with an autograft in 25 male dogs. The grafts were harvested by resection of the parietal layer of the testicular tunica vaginalis, which was transplanted into the region of the pelvi-ureteric junction and the proximal ureter...
December 2017: Urologii︠a︡
https://www.readbyqxmd.com/read/29359296/histological-changes-in-the-rat-femoral-artery-following-the-use-of-the-empty-and-refill-test
#12
Alexandra Naides, Roberto Noland, Jiajie G Lu, Yelena Akelina, Charles Marboe, Robert J Strauch
BACKGROUND:  This study examines the effects of the empty-and-refill patency test on rat femoral arteries in the longer postoperative time period. METHODS:  A simple arterial anastomosis was performed bilaterally on 20 rats. The empty-and-refill test was performed unilaterally in all rats, leaving the contralateral artery as an internal control. Rats were divided into two cohorts of 10 rats and survived for 48 hours and 2 weeks. Vessel patency was assessed prior to closing and immediately prior to sacrifice...
January 22, 2018: Journal of Reconstructive Microsurgery
https://www.readbyqxmd.com/read/29330589/impact-of-valve-less-vs-standard-insufflation-on-pneumoperitoneum-volume-inflammation-and-peritoneal-physiology-in-a-laparoscopic-sigmoid-resection-experimental-model
#13
Michele Diana, Eric Noll, Andras Legnèr, Seong-Ho Kong, Yu-Yin Liu, Luigi Schiraldi, Francesco Marchegiani, Jordan Bano, Bernard Geny, Anne-Laure Charles, Bernard Dallemagne, Véronique Lindner, Didier Mutter, Pierre Diemunsch, Jacques Marescaux
BACKGROUND: Standard insufflators compensate for intra-abdominal pressure variations with pressure spikes. Our aim was to evaluate the impact of a stable, low-pressure pneumoperitoneum induced by a valve-less insufflator, on working space, hemodynamics, inflammation, and peritoneal physiology, in a model of laparoscopic sigmoid resection. MATERIALS AND METHODS: Twelve pigs (47 ± 3.3 kg) were equipped for invasive hemodynamic monitoring and randomly assigned to Standard (n = 6) vs...
January 12, 2018: Surgical Endoscopy
https://www.readbyqxmd.com/read/29300699/a-self-calibrated-tissue-viability-sensor-for-free-flap-monitoring
#14
Melissa Berthelot, Guang-Zhong Yang, Benny Lo
In fasciocutaneous free flap surgery, close postoperative monitoring is crucial for detecting flap failure, as around 10% of cases require additional surgery due to compromised anastomosis. Different biochemical and biophysical techniques have been developed for continuous flap monitoring, however, they all have shortcoming in terms of reliability, elevated cost, potential risks to the patient, and inability to adapt to the patient's phenotype. A wearable wireless device based on near infrared spectroscopy has been developed for continuous blood flow and perfusion monitoring by quantifying tissue oxygen saturation ()...
January 2018: IEEE Journal of Biomedical and Health Informatics
https://www.readbyqxmd.com/read/29258364/comparative-study-of-notes-versus-endoscopic-ultrasound-gastrojejunostomy-in-pigs-a-prospective-study
#15
Bogdan Silviu Ungureanu, Ștefan Pătrașcu, Alice Drăgoescu, Carmen Nicolau, Cătălin Copăescu, Valeriu Șurlin, Adrian Săftoiu
BACKGROUND: Surgical gastrojejunal anastomosis (GJJ) is considered the standard palliative option for gastric outlet obstruction. The use of endoscopic GJJ has generated a lot of attention and has enlarged the horizon for patients with open surgery contraindications. Our study aimed to assess and compare 2 purely endoscopic GJJ approaches using a lumen-apposing hot tip double-flanged metal stent, with focus on technical and clinical success rates on experimental animals. METHODS: Two endoscopic GJJ techniques using a double-flanged self-expandable metal stent were compared on 8 pigs...
December 1, 2017: Surgical Innovation
https://www.readbyqxmd.com/read/29243145/the-first-consensus-statement-on-one-anastomosis-mini-gastric-bypass-oagb-mgb-using-a-modified-delphi-approach
#16
Kamal K Mahawar, Jacques Himpens, Scott A Shikora, Jean-Marc Chevallier, Mufazzal Lakdawala, Maurizio De Luca, Rudolf Weiner, Ali Khammas, Kuldeepak Singh Kular, Mario Musella, Gerhard Prager, Mohammad Khalid Mirza, Miguel Carbajo, Lilian Kow, Wei-Jei Lee, Peter K Small
BACKGROUND: An increasing number of surgeons worldwide are now performing one anastomosis/mini gastric bypass (OAGB/MGB). Lack of a published consensus amongst experts may be hindering progress and affecting outcomes. This paper reports results from the first modified Delphi consensus building exercise on this procedure. METHODS: A committee of 16 recognised opinion-makers in bariatric surgery with special interest in OAGB/MGB was constituted. The committee invited 101 OAGB/MGB experts from 39 countries to vote on 55 statements in areas of controversy or variation associated with this procedure...
December 14, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/29234924/a-new-direction-in-anastomotic-research-should-we-redesign-the-angle-of-sorrow
#17
Omar E Aly
INTRODUCTION: Despite advances in oncological outcomes in colo-rectal surgery, rates of anastomotic leak have not improved. The precise mechanisms of anastomotic leak remain poorly understood. Current research has focused on anastomotic reinforcement to tackle anastomotic leak with little success. The 'Angle of Sorrow', the corner of the anastomosis is prone to anastomotic leak, but remains a persistent feature in the gastrointestinal anastomosis. The tendency for stress forces to concentrate in the vulnerable 'Angle of Sorrow' prompts the need for anastomotic design research...
December 12, 2017: International Journal of Colorectal Disease
https://www.readbyqxmd.com/read/29205609/effect-of-tamoxifen-on-fibrosis-collagen-content-and-transforming-growth-factor-%C3%AE-1-%C3%AE-2-and-%C3%AE-3-expression-in-common-bile-duct-anastomosis-of-pigs
#18
Orlando Hiroshi Kiono Siqueira, Karen Jesus Oliveira, Angela Cristina Gouvêa Carvalho, Antonio Claudio Lucas da Nóbrega, Renata Frauches Medeiros, Bruno Felix-Patrício, Fábio Otero Áscoli, Beni Olej
End-to-end anastomosis in the treatment for bile duct injury during laparoscopic cholecystectomy has been associated with stricture formation. The aim of this study was to experimentally investigate the effect of oral tamoxifen (tmx) treatment on fibrosis, collagen content and transforming growth factor-β1, -β2 and -β3 expression in common bile duct anastomosis of pigs. Twenty-six pigs were divided into three groups [sham (n = 8), control (n = 9) and tmx (n = 9)]. The common bile ducts were transected and anastomosed in the control and tmx groups...
December 4, 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/29201224/expression-of-nf-e2-related-factor-2-in-a-rat-dural-arteriovenous-fistula-model
#19
Limin Dou, Wenhua Yu
Intracranial dural arteriovenous fistulas (DAVFs) are complex intracranial vascular malformations that may lead to hemorrhage. Although the precise mechanisms by which DAVFs occur remain unknown, dural angiogenesis may be a vital factor in its pathogenesis. Nuclear factor erythroid 2-related factor 2 (Nrf2) significantly influences angiogenesis; however, the association between DAVF and Nrf2 remains unclear. Therefore, the present study investigated whether DAVF alters the expression of Nrf2 in an experimental animal model of DAVF...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29199083/factors-correlating-with-microsurgical-performance-a-clinical-and-experimental-study
#20
Mark V Schaverien, Jun Liu, Charles E Butler, Jesse C Selber
BACKGROUND: Microsurgery is one the most complex surgical skills to master. The factors correlating with microsurgical performance, however, are poorly understood. Understanding these factors will aid in the training and assessment of microsurgeons. METHODS: A total of 29 microsurgery fellows enrolled in a dedicated 1-year microsurgery fellowship were included in the study. For the clinical evaluations, microsurgical anastomosis performance was evaluated during multiple procedures in the operating room at the start, midpoint, and end of the fellowship by all departmental faculty using a validated microsurgical assessment tool...
November 30, 2017: Journal of Surgical Education
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