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https://www.readbyqxmd.com/read/28814946/protein-signatures-of-molecular-pathways-in-non-small-cell-lung-carcinoma-nsclc-comparison-of-glycoproteomics-and-global-proteomics
#1
Shuang Yang, Lijun Chen, Daniel W Chan, Qing Kay Li, Hui Zhang
BACKGROUND: Non-small cell lung carcinoma (NSCLC) remains the leading cause of cancer deaths in the United States. More than half of NSCLC patients have clinical presentations with locally advanced or metastatic disease at the time of diagnosis. The large-scale genomic analysis of NSCLC has demonstrated that molecular alterations are substantially different between adenocarcinoma (ADC) and squamous cell carcinoma (SqCC). However, a comprehensive analysis of proteins and glycoproteins in different subtypes of NSCLC using advanced proteomic approaches has not yet been conducted...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28811020/peanut-agglutinin-and-%C3%AE-cyclodextrin-functionalized-polymer-monolith-microextraction-of-igg-galactosylation-coupled-with-online-ms-detection
#2
Haijiao Zheng, Tenggao Zhu, Xiqian Li, Jiutong Ma, Qiong Jia
A facile online method coupling polymer monolithic microextraction (PMME) with mass spectrometry (MS) was developed for the detection of Immunoglobulin G (IgG) galactosylation glycopeptides. A peanut agglutinin-β-cyclodextrin (PNA-β-CD) functionalized poly(hydroxyethyl methylacrylate-ethyleneglycol dimethacrylate) monolith was designed via a click reaction. Thanking to the specificity of PNA-β-CD for the targets, the material exhibited enhanced enrichment selectivity and extraction efficiency for IgG galactosylation glycopeptides...
August 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28802796/antibacterial-prophylaxis-for-gram-positive-and-gram-negative-infections-in-cranial-surgery-a-meta-analysis
#3
REVIEW
Pranav Abraham, Nayan Lamba, Michael Acosta, Joanna Gholmie, Hassan Y Dawood, Matthew Vestal, Kevin Huang, Maher Hulou, Morteza Asgarzadeh, Hasan Zaidi, Rania A Mekary, Timothy R Smith
BACKGROUND: Perioperative antibiotic prophylaxis against gram positive and gram negative infections is considered standard of care in the perioperative management of patients undergoing cranial surgery. The antibiotic regimen which best reduces the risk of surgical site infections (SSIs) remains controversial. OBJECTIVES: A systematic literature review and meta-analysis were conducted to examine the effect of various prophylactic antibiotics on infection incidence among patients undergoing cranial surgeries...
August 9, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28797044/what-can-we-learn-from-sonication-results-of-breast-implants
#4
Frederike M J Reischies, Robert Krause, Judith Holzer, Fabian Tiefenbacher, Raimund Winter, Gertraud Eylert, Tobias Meikl, Alexandru Tuca, Martin J Köfer, Lars P Kamolz, David B Lumenta
BACKGROUND: Different research groups have identified microorganisms on breast implants by sonication with significant correlation to the rate of capsular contracture. This substantiated the hypothesis of an infectious etiology of capsular contracture. However, no clinical consequence has been drawn from these results yet. Aim of this study was to review sonication results from breast implants and to evaluate the current preoperative antibiotic regime for breast-implant surgery. METHODS: We compared breast implant sonication culture results from published reports and our own database...
2017: PloS One
https://www.readbyqxmd.com/read/28794918/sensitive-and-structure-informative-n-glycosylation-analysis-by-maldi-ms-ionization-fragmentation-and-derivatization
#5
Takashi Nishikaze
Mass spectrometry (MS) has become an indispensable tool for analyzing post translational modifications of proteins, including N-glycosylated molecules. Because most glycosylation sites carry a multitude of glycans, referred to as "glycoforms," the purpose of an N-glycosylation analysis is glycoform profiling and glycosylation site mapping. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has unique characteristics that are suited for the sensitive analysis of N-glycosylated products...
2017: Mass Spectrometry
https://www.readbyqxmd.com/read/28793911/igg-fc-galactosylation-predicts-response-to-methotrexate-in-early-rheumatoid-arthritis
#6
Susanna L Lundström, Aase H Hensvold, Dorothea Rutishauser, Lars Klareskog, A Jimmy Ytterberg, Roman A Zubarev, Anca I Catrina
BACKGROUND: Methotrexate (MTX) is the standard first-line therapy in rheumatoid arthritis (RA) with variable clinical efficacy that is difficult to predict. The glycosylation status of immunoglobulin G (IgG) is altered in RA and influenced by MTX treatment. We aimed to further investigate if IgG glycosylation in untreated early RA can predict therapeutic response to MTX. METHODS: We used a shotgun proteomic approach to screen for the Fc glycopeptides in the serum of 12 control subjects and 59 untreated patients with early RA prior to and following MTX initiation...
August 9, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28792526/cryptosporidium-parvum-vaccine-candidates-are-incompletely-modified-with-o-linked-n-acetylgalactosamine-or-contain-n-terminal-n-myristate-and-s-palmitate
#7
John R Haserick, Joshua A Klein, Catherine E Costello, John Samuelson
Cryptosporidium parvum (studied here) and Cryptosporidium hominis are important causes of diarrhea in infants and immunosuppressed persons. C. parvum vaccine candidates, which are on the surface of sporozoites, include glycoproteins with Ser- and Thr-rich domains (Gp15, Gp40, and Gp900) and a low complexity, acidic protein (Cp23). Here we used mass spectrometry to determine that O-linked GalNAc is present in dense arrays on a glycopeptide with consecutive Ser derived from Gp40 and on glycopeptides with consecutive Thr derived from Gp20, a novel C...
2017: PloS One
https://www.readbyqxmd.com/read/28782651/emergence-of-dalbavancin-non-susceptible-vancomycin-intermediate-staphylococcus-aureus-visa-after-treatment-of-mrsa-central-line-associated-bloodstream-infection-with-a-dalbavancin-and-vancomycin-containing-regimen
#8
Brian J Werth, Rupali Jain, Andrew Hahn, Lisa Cummings, Tatiana Weaver, Adam Waalkes, Dhruba Sengupta, Stephen J Salipante, Robert M Rakita, Susan M Butler-Wu
OBJECTIVES: Dalbavancin is a long-acting lipoglycopeptide with activity against Gram-positives, including methicillin-resistant Staphylococcus aureus(MRSA). The potential for lipoglycopeptides, with half-lives greater than one-week, to select for resistance is unknown. Here we explore a case of MRSA central line-associated blood stream infection in which dalbavancin and vancomycin non-susceptibility emerged in a urine isolate collected after the patient was treated with vancomycin and dalbavancin sequentially...
August 3, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28765847/highly-efficient-enrichment-of-n-linked-glycopeptides-using-a-hydrophilic-covalent-organic-framework
#9
Yu-Fang Ma, Fang Yuan, Xiao-Hui Zhang, Ying-Lin Zhou, Xin-Xiang Zhang
The enrichment of glycopeptides plays an important role in glycoproteomics. In this paper, a covalent-organic framework called TpPa-1, synthesized by the Schiff base reaction of 1,3,5-triformylphloroglucinol and paraphenylenediamine, was first successfully utilized as a hydrophilic porous material for N-linked glycopeptide enrichment. Using this material, interference from non-glycopeptides could be efficiently eliminated, which facilitated the mass spectrometry detection of glycopeptides. By capturing N-linked glycopeptides from tryptic digests of human IgG, our method was proved to have high sensitivity at the femtomole level...
August 2, 2017: Analyst
https://www.readbyqxmd.com/read/28765562/polymeric-hydrophilic-ionic-liquids-used-to-modify-magnetic-nanoparticles-for-the-highly-selective-enrichment-of-n-linked-glycopeptides
#10
Fenglong Jiao, Fangyuan Gao, Heping Wang, Yulin Deng, Yangjun Zhang, Xiaohong Qian, Yukui Zhang
The low abundance of glycopeptides in biological samples makes it necessary to enrich them before further analysis. In this study, the polymeric hydrophilic ionic liquid-modified magnetic (Fe3O4@MPS@PMAC) nanoparticles were synthesized via a one-step reflux-precipitation polymerization. Owing to the excellent hydrophilicity and strong electrostatic interaction toward glycopeptides of the polymerized hydrophilic ionic liquid, [2-(methacryloyloxy) ethyl] trimethylammonium chloride (MAC), the synthesized Fe3O4@MPS@PMAC nanoparticles exhibited outstanding performance in glycopeptide enrichment with high detection sensitivity (10 fmol), large binding capacity (100 μg mg(-1)) and satisfied enrichment recovery (approximately 82%)...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28756579/evaluation-of-effects-of-an-operational-multidisciplinary-team-on-antibiotic-use-in-the-medium-to-long-term-at-a-french-university-hospital
#11
Béatrice Demoré, Pauline Humbert, Emmanuelle Boschetti, Sibylle Bevilacqua, Isabelle Clerc-Urmès, Thierry May, Céline Pulcini, Nathalie Thilly
Background Antibiotic-resistant bacteria are a major public health problem throughout the world. In 2006, in accordance with the national guidelines for antibiotic use, the CHRU of Nancy created an operational multidisciplinary antibiotic team at one of its sites. In 2011, a cluster-controlled trial showed that the operational multidisciplinary antibiotic team (the intervention) had a favourable short-term effect on antibiotic use and costs. Objective Our objective was to determine whether these effects continued over the medium to long term (that is, 2-7 years after creation of the operational multidisciplinary antibiotic team, 2009-2014)...
July 29, 2017: International Journal of Clinical Pharmacy
https://www.readbyqxmd.com/read/28754460/virulence-and-antibiotic-resistance-of-staphylococcus-aureus-isolated-from-bloodstream-infections-and-pneumonia-in-southern-poland
#12
Monika Pomorska-Wesołowska, Agnieszka Chmielarczyk, Monika Chlebowicz, Grzegorz Ziółkowski, Anna Szczypta, Joanna Natkaniec, Dorota Romaniszyn, Monika Pobiega, Mirosława Dzikowska, Lech Krawczyk, Joanna Kozioł, Jadwiga Wójkowska-Mach
OBJECTIVES: Staphylococcus aureus remains the most important cause of infections in hospitals and long-term care facilities.The aim of this study was to analyse the resistance, virulence and the epidemiological and genetic relationship of SA from bloodstream infections and pneumonia from patients in Southern Poland. METHODS: All strains were tested using disk diffusion antimicrobial susceptibility methods. E-tests for vancomycin, teicoplanin, tygecycline, oxacillin, cefoxitin and penicillin were also performed...
July 25, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28752167/a-novel-enzymatic-method-for-synthesis-of-glycopeptides-carrying-natural-eukaryotic-n-glycans
#13
Yangyang Xu, Zhigang Wu, Peiru Zhang, He Zhu, Hailiang Zhu, Qitao Song, Li Wang, Faxing Wang, Peng George Wang, Jiansong Cheng
A novel enzymatic approach was developed for facile production of glycopeptides carrying natural eukaryotic N-glycans. In this approach, peptides can be GlcNAcylated at one or two natural N-glycosylation sites via two-step enzymatic reactions catalyzed by an evolved N-glycosyltransferase (ApNGT(Q469A)) and a glucosamine N-acetyltransferase (GlmA), respectively. The resulting GlcNAc-peptides were further modified by an endo-β-N-acetylglucosaminidase M mutant (EndoM(N175Q)) to generate glycopeptides. In three steps of enzymatic catalysis, glycopeptides carrying complex-type N-glycans can be efficiently synthesized...
July 28, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28749714/risk-factors-for-carbapenem-resistant-klebsiella-pneumoniae-infection-a-meta-analysis
#14
Pin Liu, Xuan Li, Mei Luo, Xuan Xu, Kewen Su, Shuai Chen, Ying Qing, Yingli Li, Jingfu Qiu
AIMS: Carbapenem-resistant Klebsiella pneumoniae (CRKP) infection has been rapidly emerging as a life-threatening nosocomial disease in many countries. However, studies on the corresponding risk factors of CRKP infection showed inconsistent results. To resolve these inconsistencies, we conducted a meta-analysis of previous studies on the potential risk factors of CRKP infection. The results of this study could be used to develop CRKP infection prevention strategies. METHODS: Relevant works were systematically searched from five electronic databases up to September 2016...
July 27, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28748309/interaction-of-zn-ii-bleomycin-a2-and-zn-ii-peplomycin-with-a-dna-hairpin-containing-the-5-gt-3-binding-site-in-comparison-with-the-5-gc-3-binding-site-studied-by-nmr-spectroscopy
#15
Shelby E Follett, Azure D Ingersoll, Sally A Murray, Teresa M Reilly, Teresa E Lehmann
Bleomycins are a group of glycopeptide antibiotics synthesized by Streptomyces verticillus that are widely used for the treatment of various neoplastic diseases. These antibiotics have the ability to chelate a metal center, mainly Fe(II), and cause site-specific DNA cleavage. Bleomycins are differentiated by their C-terminal regions. Although this antibiotic family is a successful course of treatment for some types of cancers, it is known to cause pulmonary fibrosis. Previous studies have identified that bleomycin-related pulmonary toxicity is linked to the C-terminal region of these drugs...
July 26, 2017: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/28745859/quantitative-effects-of-o-linked-glycans-on-protein-folding
#16
Patrick K Chaffey, Xiaoyang Guan, Xinfeng Wang, Yuan Ruan, Yaohao Li, Suzannah G Miller, Amy H Tran, Theo N Koelsch, Lomax F Pass, Zhongping Tan
Protein O-glycosylation is a diverse, common, and important post-translational modification of both proteins inside the cell and those that are secreted or membrane bound. Much work has shown that O-glycosylation can alter the structure, func-tion, and physical properties of the proteins to which it is attached. One gap remaining in our understanding of O-glycoproteins is how O-glycans might affect the folding of proteins. Here, we took advantage of synthetic, homogenous O-glycopeptides to show that certain glycosylation patterns have an intrinsic effect, independent of any cellular folding machinery, on the folding pathway of a model O-glycoprotein, a carbohydrate binding module (CBM) derived from the Trichoderma reesei cellulase TrCel7A...
July 26, 2017: Biochemistry
https://www.readbyqxmd.com/read/28743428/a-therapeutic-benefit-of-daptomycin-against-glycopeptide-resistant-gram-positive-cocci-bloodstream-infections-under-neutropenia
#17
Kensuke Matsuda, Junji Koya, Kazuhiro Toyama, Mahoko Ikeda, Shunya Arai, Fumihiko Nakamura, Shu Okugawa, Kyoji Moriya, Mineo Kurokawa
Antibiotic-resistant infections remain to be a major issue for all over the world. Although appropriate diagnosis and rapid treatment initiation are crucially important particularly in immunocompromised patients, selection of antibiotics without identification of causative bacteria is often challenging. A 44-year-old woman with acute myeloid leukemia (AML) under myelosuppression suffered from teicoplanin-resistant gram-positive cocci bacteremia. Taking severe neutropenia due to chemotherapy and glycopeptide-resistance into account, teicoplanin was empirically substituted with daptomycin, which led to prompt defervescence...
July 22, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28741944/substrate-recognition-of-glycoprotein-folding-sensor-uggt-analyzed-by-site-specifically-15-n-labeled-glycopeptide-and-small-glycopeptide-library-prepared-by-parallel-native-chemical-ligation
#18
Masayuki Izumi, Rie Kuruma, Ryo Okamoto, Akira Seko, Yukishige Ito, Yasuhiro Kajihara
UDP-glucose:glycoprotein glucosyltransferase (UGGT) distinguishes glycoproteins in non-native conformations from those in native conformations and glucosylates from only non-native glycoproteins. To analyze how UGGT recognizes non-native glycoproteins, we chemically synthesized site-specifically (15)N-labeled interleukin 8 (IL-8) C-terminal (34-72) glycopeptides bearing a Man9GlcNAc2 (M9) oligosaccharide. Chemical shift perturbation mapping NMR experiments suggested that Phe65 of the glycopeptide specifically interacts with UGGT...
August 10, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28737213/identification-of-methylated-gnti-dependent-n-glycans-in-botryococcus-brauni
#19
Stefan Schulze, Eugen Urzica, Maarten J M F Reijnders, Henri van de Geest, Sven Warris, Linda V Bakker, Christian Fufezan, Vitor A P Martins Dos Santos, Peter J Schaap, Sander A Peters, Michael Hippler
In contrast to mammals and vascular plants, microalgae show a high diversity in the N-glycan structures of complex N-glycoproteins. Although homologues for β1,2-N-acetylglucosaminyltransferase I (GnTI), a key enzyme in the formation of complex N-glycans, have been identified in several algal species, GnTI-dependent N-glycans have not been detected so far. We have performed an N-glycoproteomic analysis of the hydrocarbon oils accumulating green microalgae Botryococcus braunii. Thereby, the analysis of intact N-glycopeptides allowed the determination of N-glycan compositions...
September 2017: New Phytologist
https://www.readbyqxmd.com/read/28736752/inhibitory-activity-of-a-concanavalin-isolated-fraction-from-a-glucosamine-peptides-reaction-system-against-heat-resistant-e-coli
#20
Yuliya Hrynets, Daylin Johana Hincapie Martinez, Maurice Ndagijimana, Mirko Betti
Alcalase-derived gelatin hydrolysates were glycated with glucosamine in the presence (+) or absence (-) of transglutaminase (TGase), and their antimicrobial activities toward Escherichia coli AW 1.7 were studied. Glycation treatments were subjected to concanavalin A affinity chromatography to selectively collect the glycopeptide-enriched fractions and the changes in antimicrobial activity were determined. The minimum inhibitory concentration of glycated hydrolysates decreased by 1.2 times compared to the native hydrolysate, with no differences between (+) or (-) TGase treatments...
July 2017: Heliyon
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