keyword
https://read.qxmd.com/read/38535625/targeted-and-self-adjuvated-nanoglycovaccine-candidate-for-cancer-immunotherapy
#1
JOURNAL ARTICLE
Rui Freitas, Eduardo Ferreira, Andreia Miranda, Dylan Ferreira, Marta Relvas-Santos, Flávia Castro, Beatriz Santos, Martina Gonçalves, Sofia Quintas, Andreia Peixoto, Carlos Palmeira, André M N Silva, Lúcio Lara Santos, Maria José Oliveira, Bruno Sarmento, José Alexandre Ferreira
Advanced-stage solid primary tumors and metastases often express mucin 16 (MUC16), carrying immature glycans such as the Tn antigen, resulting in specific glycoproteoforms not found in healthy human tissues. This presents a valuable approach for designing targeted therapeutics, including cancer glycovaccines, which could potentially promote antigen recognition and foster the immune response to control disease spread and prevent relapse. In this study, we describe an adjuvant-free poly(lactic- co -glycolic acid) (PLGA)-based nanoglycoantigen delivery approach that outperforms conventional methods by eliminating the need for protein carriers while exhibiting targeted and adjuvant properties...
March 27, 2024: ACS Nano
https://read.qxmd.com/read/38535549/genomic-insights-into-staphylococcus-aureus-isolates-exhibiting-diminished-daptomycin-susceptibility
#2
JOURNAL ARTICLE
Natalia Gómez-Casanova, Mª Nieves Gutiérrez-Zufiaurre, Ana Mª Blázquez de Castro, Juan Luis Muñoz-Bellido
Daptomycin is one of the last therapeutic resources for multidrug-resistant gram-positive bacteria. Despite its structural similarities with glycopeptides, its mechanisms of action and resistance are different and in some respects are not completely understood. Mutations in several genes have been associated with daptomycin resistance, especially in mprF , walkR , rpoB and rpoC , but their role and importance remain to be elucidated. We have studied mutations in 11 genes, which have been previously associated with daptomycin non-susceptibility, in nine daptomycin-non-susceptible Staphylococcus aureus clinical isolates (daptomycin MIC: >1 mg/L)...
February 26, 2024: Pathogens
https://read.qxmd.com/read/38530540/the-functional-study-of-response-regulator-arlr-mutants-in-staphylococcus-aureus
#3
JOURNAL ARTICLE
Jinhong Zhou, Moath Refat, Yucheng Guo, Jiaxin Zhang, Min Jiao, Wenbo He, Xiaoyu He, Mai A Rabie, Zhenlin Ouyang, Fang Zheng
Staphylococcus aureus is a major cause of hospital-associated infections worldwide. The organism's ability to form biofilms has led to resistance against current treatment options such as beta-lactams, glycopeptides, and daptomycin. The ArlRS two-component system is a crucial regulatory system necessary for S. aureus autolysis, biofilm formation, capsule synthesis, and virulence. This study aims to investigate the role of the arlR deletion mutant in the detection and activation of S. aureus. We created an arlR deleted mutant and complementary strains and characterized their impact on the strains using partial growth measurement...
March 26, 2024: Applied Biochemistry and Biotechnology
https://read.qxmd.com/read/38529842/emulsion-template-fabricated-heterogeneous-bilayer-gelatin-based-scaffolds-with-sustained-delivery-of-lycium-barbarum-glycopeptide-for-periodontitis-treatment
#4
JOURNAL ARTICLE
Siqi He, Nan Wen, Xun Chen, Cong Liu, Xun Xiao, Xinlun Li, Lun Yuan, Yandong Mu
Periodontitis is a chronic inflammatory disease raising the risks of tooth-supporting structures destruction and even tooth loss. The way to reconstruct periodontal bone tissues in inflammatory microenvironment has been long in demand for periodontitis treatment. In this study, the lycium barbarum glycopeptide (LbGP) loaded gelatin-based scaffolds were fabricated for periodontitis treatment. Gelatin microspheres with suitable size were prepared by emulsification and gathered by oxidized sodium alginate to prepare heterogeneous bilayer gelatin-based scaffolds, and then they were loaded with LbGP...
March 26, 2024: Journal of Biomaterials Science. Polymer Edition
https://read.qxmd.com/read/38523065/self-promoted-glycosylation-of-carbamoylated-peptides-on-solid-phase
#5
JOURNAL ARTICLE
Niklas Fischer, Benedicte Vævest, Andreas Ketilsø Dam, Frederik Diness, Christian Marcus Pedersen
Self-promoted glycosylations with trichloroacetimidate glycosyl donors are demonstrated on solid-phase-anchored peptides orthogonally deprotected and tosylcarbamoylated on the side-chains of cysteine and serine, respectively. The donor scope included glucosyl as well as mannosyl trichloroacetimidates, carrying benzyl, acetyl, or isopropylidene protecting groups. Isopropylidene groups were found to be removed under the acidic conditions used for release of the neoglycopeptides from the solid support, yielding neoglycopeptides with unprotected hydroxyl groups...
March 24, 2024: ChemPlusChem
https://read.qxmd.com/read/38518846/risk-factors-for-resistant-gram-positive-bacteremia-in-febrile-neutropenic-patients-with-cancer
#6
JOURNAL ARTICLE
Minkyeong Lee, Chan Mi Lee, Ja Min Byun, Dong-Yeop Shin, Youngil Koh, Junshik Hong, Pyoeng Gyun Choe, Wan Beom Park, Nam Joong Kim, Sung-Soo Yoon, Myoung-Don Oh, Chang Kyung Kang, Inho Kim
BACKGROUND: Gram-positive bacteria are frequently resistant to empirical beta-lactams in febrile neutropenic patients with cancer. As microbiology and antibiotic susceptibility changes, we reevaluated the risk factors for resistant Gram-positive bacteremia in febrile neutropenic patients with cancer. METHODS: Episodes of bacteremic febrile neutropenia in Seoul National University Hospital from July 2019 to June 2022 were reviewed. Resistant Gram-positive bacteria were defined as a pathogen susceptible only to glycopeptide or linezolid in vitro (e...
March 20, 2024: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://read.qxmd.com/read/38518664/integrated-landscape-of-chicken-egg-chalaza-proteomics
#7
JOURNAL ARTICLE
Jing Pu, Jian Hu, Jing Xiao, Shugang Li, Beibei Wang, Jinqiu Wang, Fang Geng
Chicken egg chalaza (CLZ) is a natural colloidal structure in eggs that exists as an egg yolk stabilizer and is similar in composition to egg white. In this study, the proteome, phosphoproteome, and N-glycoproteome of CLZ were characterized in depth. We hydrolyzed the CLZ proteins and enriched the phosphopeptides and glycopeptides. We identified 45 phosphoproteins and 80 N-glycoproteins, containing 59 phosphosites and 203 N-glycosylation sites, respectively. Typically, the ovalbumin in CLZ was both phosphorylated and N-glycosylated, with 4 phosphosites and 4 N-glycosylation sites...
March 7, 2024: Poultry Science
https://read.qxmd.com/read/38513651/mass-spectrometry-analysis-of-glycopeptides-enriched-by-anion-exchange-mediated-methods-reveals-polylacnac-extended-n-glycans-in-integrins-and-tetraspanins-in-melanoma-cells
#8
JOURNAL ARTICLE
Tomislav Čaval, Gege Xu, Maryam Baniasad, Chih-Wei Chu, Rachel Rice, Itati Hundal, Gregg Czerwieniec, Flavio Schwarz
Glycosylation is a key modulator of the functional state of proteins. Recent developments in large-scale analysis of intact glycopeptides have enabled the identification of numerous glycan structures that are relevant in pathophysiological processes. However, one motif found in N -glycans, poly- N -acetyllactosamine (polyLacNAc), still poses a substantial challenge to mass spectrometry-based glycoproteomic analysis due to its relatively low abundance and large size. In this work, we developed approaches for the systematic mapping of polyLacNAc-elongated N -glycans in melanoma cells...
March 21, 2024: Analytical Chemistry
https://read.qxmd.com/read/38513100/directed-evolution-of-genetically-encoded-lytacs-for-cell-mediated-delivery
#9
JOURNAL ARTICLE
Jonathan Lee Yang, Sean A Yamada-Hunter, Louai Labanieh, Elena Sotillo, Joleen S Cheah, David S Roberts, Crystal L Mackall, Carolyn R Bertozzi, Alice Y Ting
Lysosome-targeting chimeras (LYTACs) are a promising therapeutic modality to drive the degradation of extracellular proteins. However, early versions of LYTAC contain synthetic glycopeptides that cannot be genetically encoded. Here, we present our designs for a fully genetically encodable LYTAC (GELYTAC), making our tool compatible with integration into therapeutic cells for targeted delivery at diseased sites. To achieve this, we replaced the glycopeptide portion of LYTACs with the protein insulin-like growth factor 2 (IGF2)...
March 26, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38512228/mass-spectrometry-compatible-elution-technique-enables-an-improved-mucin-selective-enrichment-strategy-to-probe-the-mucinome
#10
JOURNAL ARTICLE
Keira E Mahoney, Vincent Chang, Taryn M Lucas, Krystyna Maruszko, Stacy A Malaker
Mucin-domain glycoproteins are densely O-glycosylated and play critical roles in a host of healthy and disease-driven biological functions. Previously, we developed a mucin-selective enrichment strategy by employing a catalytically inactive mucinase (StcE) conjugated to a solid support. While this method was effective, it suffered from low throughput and high sample requirements. Further, the elution step required boiling in SDS, thus necessitating an in-gel digest with trypsin. Here, we introduce innovative elution conditions amenable to mucinase digestion and downstream analysis using mass spectrometry...
March 21, 2024: Analytical Chemistry
https://read.qxmd.com/read/38503734/prediction-of-glycopeptide-fragment-mass-spectra-by-deep-learning
#11
JOURNAL ARTICLE
Yi Yang, Qun Fang
Deep learning has achieved a notable success in mass spectrometry-based proteomics and is now emerging in glycoproteomics. While various deep learning models can predict fragment mass spectra of peptides with good accuracy, they cannot cope with the non-linear glycan structure in an intact glycopeptide. Herein, we present DeepGlyco, a deep learning-based approach for the prediction of fragment spectra of intact glycopeptides. Our model adopts tree-structured long-short term memory networks to process the glycan moiety and a graph neural network architecture to incorporate potential fragmentation pathways of a specific glycan structure...
March 19, 2024: Nature Communications
https://read.qxmd.com/read/38503008/determining-steady-state-trough-range-in-vancomycin-drug-dosing-using-machine-learning
#12
JOURNAL ARTICLE
M Samie Tootooni, Erin F Barreto, Phichet Wutthisirisart, Kianoush B Kashani, Kalyan S Pasupathy
BACKGROUND: Vancomycin is a renally eliminated, nephrotoxic, glycopeptide antibiotic with a narrow therapeutic window, widely used in intensive care units (ICU). We aimed to predict the risk of inappropriate vancomycin trough levels and appropriate dosing for each ICU patient. METHODS: Observed vancomycin trough levels were categorized into sub-therapeutic, therapeutic, and supra-therapeutic levels to train and compare different classification models. We included adult ICU patients (≥ 18 years) with at least one vancomycin concentration measurement during hospitalization at Mayo Clinic, Rochester, MN, from January 2007 to December 2017...
March 18, 2024: Journal of Critical Care
https://read.qxmd.com/read/38488563/lycium-barbarum-glycopeptide-wolfberry-extract-slows-n-methyl-n-nitrosourea-induced-degradation-of-photoreceptors
#13
JOURNAL ARTICLE
Qihang Kong, Xiu Han, Haiyang Cheng, Jiayu Liu, Huijun Zhang, Tangrong Dong, Jiansu Chen, Kwok-Fai So, Xuesong Mi, Ying Xu, Shibo Tang
JOURNAL/nrgr/04.03/01300535-202410000-00030/figure1/v/2024-02-06T055622Z/r/image-tiff Photoreceptor cell degeneration leads to blindness, for which there is currently no effective treatment. Our previous studies have shown that Lycium barbarum (L. barbarum) polysaccharide (LBP) protects degenerated photoreceptors in rd1, a transgenic mouse model of retinitis pigmentosa. L. barbarum glycopeptide (LbGP) is an immunoreactive glycoprotein extracted from LBP. In this study, we investigated the potential protective effect of LbGP on a chemically induced photoreceptor-degenerative mouse model...
October 1, 2024: Neural Regeneration Research
https://read.qxmd.com/read/38486989/the-role-of-n-glycosylation-in-cancer
#14
REVIEW
Yu Lin, David M Lubman
Despite advances in understanding the development and progression of cancer in recent years, there remains a lack of comprehensive characterization of the cancer glycoproteome. Glycoproteins play an important role in medicine and are involved in various human disease conditions including cancer. Glycan-moieties participate in fundamental cancer processes like cell signaling, invasion, angiogenesis, and metastasis. Aberrant N -glycosylation significantly impacts cancer processes and targeted therapies in clinic...
March 2024: Acta Pharmaceutica Sinica. B
https://read.qxmd.com/read/38479167/improving-anti-hiv-activity-and-pharmacokinetics-of-enfuvirtide-t20-by-modification-with-oligomannose
#15
JOURNAL ARTICLE
Shuihong Cheng, Mingyue Xu, Mingli Li, Yong Feng, Lin He, Tong Liu, Liying Ma, Xuebing Li
Dendritic cells (DCs) play a pivotal role in controlling HIV-1 infections of CD4+ T cells. DC-SIGN, which is expressed on the surface of DCs, efficiently captures HIV-1 virions by binding to the highly mannosylated membrane protein, gp120, and then the DCs transport the virus to target T cells in lymphoid organs. This study explored the modification of T20, a peptide inhibitor of HIV-1 fusion, by conjugation of the N-terminus with varying sizes of oligomannose, which are DC-SIGN-specific carbohydrates, aiming to create dual-targeting HIV inhibitors...
March 8, 2024: European Journal of Medicinal Chemistry
https://read.qxmd.com/read/38471429/comparative-analysis-of-antimicrobial-resistance-phenotype-and-genotype-of-riemerella-anatipestifer
#16
JOURNAL ARTICLE
Hongyan Dong, Shanyuan Zhu, Fan Sun, Qi Feng, Changming Guo, Zhi Wu, Shuang Wu, Anping Wang, Shengqing Yu
Riemerella anatipestifer is one of the important bacterial pathogens that threaten the waterfowl farming industry. In this study, 157 suspected R. anatipestifer strains were isolated from diseased ducks and geese from seven regions of China during 2019-2020, and identified using multiple polymerase chain reaction (PCR). Antimicrobial susceptibility tests and whole-genome sequence (WGS) analysis were then performed for comparative analysis of antimicrobial resistance phenotypes and genotypes. The results showed that these strains were susceptible to florfenicol, ceftriaxone, spectinomycin, sulfafurazole and cefepime, but resistant to kanamycin, amikacin, gentamicin, and streptomycin, exhibiting multiple antimicrobial resistance phenotypes...
March 8, 2024: Veterinary Microbiology
https://read.qxmd.com/read/38470198/dysregulated-proteome-and-n-glycoproteome-in-alg1-deficient-fibroblasts
#17
JOURNAL ARTICLE
Rohit Budhraja, Neha Joshi, Silvia Radenkovic, Tamas Kozicz, Eva Morava, Akhilesh Pandey
Asparagine-linked glycosylation 1 protein is a β-1,4-mannosyltransferase, is encoded by the ALG1 gene, which catalyzes the first step of mannosylation in N-glycosylation. Pathogenic variants in ALG1 cause a rare autosomal recessive disorder termed as ALG1-CDG. We performed a quantitative proteomics and N-glycoproteomics study in fibroblasts derived from patients with one homozygous and two compound heterozygous pathogenic variants in ALG1. Several proteins that exhibited significant upregulation included insulin-like growth factor II and pleckstrin, whereas hyaluronan and proteoglycan link protein 1 was downregulated...
March 12, 2024: Proteomics
https://read.qxmd.com/read/38465850/effect-of-institutional-antimicrobial-stewardship-guidelines-on-prescription-of-critically-important-antimicrobials-for-dogs-and-cats
#18
JOURNAL ARTICLE
Sarah N Robbins, Robert Goggs, Sydney Kraus-Malett, Laura Goodman
BACKGROUND: Veterinary hospital antimicrobial stewardship (AMS) guidelines might help combat antimicrobial resistance (AMR). OBJECTIVES: Determine the conditions and types of infection for which antimicrobial drugs (AMDs) deemed critically important (CIA) by the World Health Organization (WHO) were prescribed and assess the effect of hospital AMS guidelines on adherence to International Society for Companion Animal Infectious Diseases published guidelines for the treatment of superficial bacterial folliculitis, respiratory tract disease and urinary tract infection in these cases...
March 11, 2024: Journal of Veterinary Internal Medicine
https://read.qxmd.com/read/38462042/microenvironment-responsive-smart-hydrogels-with-antibacterial-activity-and-immune-regulation-for-accelerating-chronic-wound-healing
#19
JOURNAL ARTICLE
Xiangtian Deng, Ye Wu, YunFeng Tang, Zilu Ge, Dong Wang, Cheng Zheng, Renliang Zhao, Wei Lin, Guanglin Wang
Current therapeutic strategies for chronic refractory wounds remain challenge owing to their unfavorable wound microenvironment and poor skin regeneration ability. Thus far, a regimen for effective chronic refractory wounds management involves bacterial elimination, alleviation of oxidative stress, inhibition of inflammatory response, and promotion of angiogenesis. In this work, an injectable glycopeptide hydrogel based on phenylboronic acid-grafted ϵ-polylysine (EPBA) and poly (vinyl alcohol) (PVA) with pH/reactive oxygen species (ROS) dual-responsive properties was prepared, which exerted intrinsic antibacterial and antioxidant properties...
March 8, 2024: Journal of Controlled Release
https://read.qxmd.com/read/38456960/lectin-a-molecular-tool-in-cancer-diagnosis-and-therapy-with-special-reference-to-reproductive-cancers
#20
REVIEW
Rachna M Pallar, Shubhangi K Pingle, Avinash Shivaji Gaikwad, Naveen S Yennam, N Raju, Panja Kumar, Vinay Kumar Adepu, Rajani G Tumane, Chennuru Veeranjaneyulu, Kartikey Matte
The prevalence of cancer deaths globally and domestically is higher especially due to the deferment of diagnosis and lack of facilities for women's reproductive cancers. The present review focussed to explore the application of lectins in cancer theranostics. Though there is cancer diagnostic and treatment available there is no promising early diagnostic tool and effective treatment available for the cancer which is the major concern. Lectins are cellulose-binding proteins that are strongly determined in saccharide groups of glycans, glycopeptides, or glycolipids...
March 8, 2024: Molecular Biotechnology
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