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https://www.readbyqxmd.com/read/29670890/correlations-between-microbial-communities-in-stool-and-clinical-indicators-in-patients-with-metabolic-syndrome
#1
Lang Lin, Zai-Bo Wen, Dong-Jiao Lin, Jiang-Ting Dong, Jie Jin, Fei Meng
AIM: To analyze the bacterial community structure and distribution of intestinal microflora in people with and without metabolic syndrome and combined these data with clinical indicators to determine relationships between selected bacteria and metabolic diseases. METHODS: Faecal samples were collected from 20 patients with metabolic syndrome and 16 controls at Cangnan People's Hospital, Zhejiang Province, China. DNA was extracted and the V3-V4 regions of the 16S rRNA genes were amplified for high throughput sequencing...
April 16, 2018: World Journal of Clinical Cases
https://www.readbyqxmd.com/read/29668903/the-interplay-between-oral-microbiome-lifestyle-factors-and-genetic-polymorphisms-in-the-risk-of-oral-squamous-cell-carcinoma
#2
Jenn-Ren Hsiao, Chan-Chi Chang, Wei-Ting Lee, Cheng-Chih Huang, Chun-Yen Ou, Sen-Tien Tsai, Ken-Chung Chen, Jehn-Shyun Huang, Tung-Yiu Wong, Yu-Hsuan Lai, Yuan-Hua Wu, Wei-Ting Hsueh, Shang-Yin Wu, Chia-Jui Yen, Jang-Yang Chang, Chen-Lin Lin, Ya-Ling Weng, Han-Chien Yang, Yu-Shan Chen, Jeffrey S Chang
Poor oral hygiene may lead to overgrowth of pathogenic oral bacteria, which may induce chronic inflammation to promote the oncogenesis of oral squamous cell carcinoma (OSCC). This study investigated the association between oral bacterial profile and OSCC risk in a case-control study of 138 OSCC cases and 151 controls (88 cases and 90 controls for the discovery group and 50 cases and 61 controls for the validation group). Oral bacterial profiles were characterized by targeted sequencing of the 16S rRNA gene...
April 13, 2018: Carcinogenesis
https://www.readbyqxmd.com/read/29668623/differential-expressions-of-biomarkers-in-gingival-crevicular-fluid-of-han-and-uygur-populations-with-peri-implantitis
#3
Xiaowei Gao, Jing Zhou, Yue Sun, Lin Wang, Yanmin Zhou
This study aims to investigate and compare the biomarkers in the gingival crevicular fluid between the Han and Uygur subjects with healthy implants and peri-implantitis.Totally 80 subjects were divided into the H-case (Han patients with peri-implantitis), U-case (Uygur patients with peri-implantitis), H-control (Han subjects with healthy implants), and U-control (Uygur subjects with healthy implants) groups. Cytokine levels in the gingival crevicular fluid were detected, and the dominant bacteria species were analyzed...
April 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29668250/the-effects-of-baicalein-on-cortical-pro-inflammatory-cytokines-and-the-intestinal-microbiome-in-senescence-accelerated-mouse-prone-8
#4
Li Gao, Jia-Qi Li, Yu-Zhi Zhou, Xudong Huang, Xue-Mei Qin, Guan-Hua Du
Baicalein, a flavonoid derived from the roots of Scutellariae baicalensis Georgi, has shown health benefits for an array of human diseases including dementia. The senescence-accelerated mouse prone 8 (SAMP8) strain is extensively used as a senile dementia model. To further investigate the effects of baicalein in SAMP8 mice, behavioral testing, biochemical detection and gut microbiota analysis were performed. The results demonstrated that treatment with baicalein ameliorated the senescence status of the SAMP8 mice, as manifested by reducing the grading score of senescence...
April 18, 2018: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29667264/metagenomic-analysis-of-oral-microbiome-in-young-children-aged-6-8-y-living-in-a-rural-isolated-chinese-province
#5
Yuan Xu, Yuhuan Jia, Liang Chen, Wenming Huang, Deqin Yang
BACKGROUND: The mixed dentition is an important transition period from primary teeth. to permanent teeth. However, the caries prevalence of first permanent molar in mixed dentitions was about 30%, which almost represent the caries rate of permanent teeth in this period of time. Therefore, we assessed the oral bacterial profiles in young children (age 6-8) with mixed dentition with or without first molar caries for providing the research basis of caries etiology. METHODS: We collected samples of supragingival plaque and saliva from the children living in Guizhou, a rural isolated province in China...
April 18, 2018: Oral Diseases
https://www.readbyqxmd.com/read/29665003/the-inside-tract-the-appendicular-cecal-and-colonic-microbiome-of-captive-aye-ayes
#6
Lydia K Greene, Erin A McKenney
OBJECTIVES: The aye-aye (Daubentonia madagascariensis) is famous for its feeding strategies that target structurally defended, but high-quality resources. Nonetheless, the influence of this digestible diet on gut microbial contributions to aye-aye metabolism and nutrition remains unexplored. When four captive aye-ayes were unexpectedly lost to persin toxicity, we opportunistically collected samples along the animals' gastrointestinal tracts. Here we describe the diversity and composition of appendicular, cecal, and colonic consortia relative to the aye-aye's unusual feeding ecology...
April 17, 2018: American Journal of Physical Anthropology
https://www.readbyqxmd.com/read/29662480/comparative-analysis-of-the-microbiota-between-sheep-rumen-and-rabbit-cecum-provides-new-insight-into-their-differential-methane-production
#7
Lan Mi, Bin Yang, Xialu Hu, Yang Luo, Jianxin Liu, Zhongtang Yu, Jiakun Wang
The rumen and the hindgut represent two different fermentation organs in herbivorous mammals, with the former producing much more methane than the latter. The objective of this study was to elucidate the microbial underpinning of such differential methane outputs between these two digestive organs. Methane production was measured from 5 adult sheep and 15 adult rabbits, both of which were placed in open-circuit respiratory chambers and fed the same diet (alfalfa hay). The sheep produced more methane than the rabbits per unit of metabolic body weight, digestible neutral detergent fiber, and acid detergent fiber...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29659523/antibacterial-efficacy-of-silver-nanoparticles-on-endometritis-caused-by-prevotella-melaninogenica-and-arcanobacterum-pyogenes-in-dairy-cattle
#8
Sangiliyandi Gurunathan, Yun-Jung Choi, Jin-Hoi Kim
Bovine postpartum diseases remain one of the most significant and highly prevalent illnesses with negative effects on the productivity, survival, and welfare of dairy cows. Antibiotics are generally considered beneficial in the treatment of endometritis; however, frequent usage of each antibiotic drug is reason for the emergence of multidrug resistance (MDR) of the pathogenic microorganisms, representing a major impediment for the successful diagnosis and management of infectious diseases in both humans and animals...
April 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29658026/oral-administration-of-rutile-and-anatase-tio-2-nanoparticles-shifts-mouse-gut-microbiota-structure
#9
Juan Li, Shengmei Yang, Runhong Lei, Weihong Gu, Yanxia Qin, Sihan Ma, Kui Chen, Yanan Chang, Xue Bai, Shibo Xia, Chongming Wu, Gengmei Xing
The widespread application of TiO2 nanoparticles (NPs) as additives in foods such as gum, candy and puddings has dramatically increased the human ingestion and accumulation of these nanomaterials. Although the toxicity of TiO2 NPs has been extensively studied, their impact on gut microbiota in vivo still needs further research. In this study, TiO2 NPs with two main crystalline phases anatase and rutile were orally administrated to mice for 28 days. The dynamic influences of anatase and rutile on gut microbiota structures were investigated at doses equivalent to those consumed by people who love to eat candies...
April 16, 2018: Nanoscale
https://www.readbyqxmd.com/read/29653422/high-throughput-sequencing-reveals-microbial-communities-in-drinking-water-treatment-sludge-from-six-geographically-distributed-plants-including-potentially-toxic-cyanobacteria-and-pathogens
#10
Hangzhou Xu, Haiyan Pei, Yan Jin, Chunxia Ma, Yuting Wang, Jiongming Sun, Hongmin Li
The microbial community structures of drinking water treatment sludge (DWTS) generated for raw water (RW) from different locations and with different source types - including river water, lake water and reservoir water -were investigated using high-throughput sequencing. Because the unit operations in the six DWTPs were similar, community composition in fresh sludge may be determined by microbial community in the corresponding RW. Although Proteobacteria, Cyanobacteria, Bacteroidetes, Firmicutes, Verrucomicrobia, and Planctomycetes were the dominant phyla among the six DWTS samples, no single phylum exhibited similar abundance across all the samples, owing to differences in total phosphorus, chemical oxygen demand, Al, Fe, and chloride in RW...
April 10, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29653193/treatment-of-periodontitis-in-smokers-with-multiple-sessions-of-antimicrobial-photodynamic-therapy-or-systemic-antibiotics-a-randomized-clinical-trial
#11
Letícia Helena Theodoro, Naida Zanini Assem, Mariéllen Longo, Márcio Luiz Ferro Alves, Cristiane Duque, Rafael Nobrega Stipp, Natália Leal Vizoto, Valdir Gouveia Garcia
BACKGROUND: The aim of this study was to evaluate the effects of non-surgical periodontal therapies on smokers with chronic periodontitis, involving multiple adjunctive applications of antimicrobial photodynamic therapy (aPDT), and systemic metronidazole (MTZ) with amoxicillin (AMX). METHODS: All participants were treated with scaling and root planing (SRP). Seventeen patients received 400 mg of MTZ and 500 mg of AMX three times per day for 7 days (MTZ + AMX)...
April 10, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29651696/diversity-of-the-midstream-urine-microbiome-in-adults-with-chronic-kidney-disease
#12
Holly Kramer, Gina Kuffel, Krystal Thomas-White, Alan J Wolfe, Kavitha Vellanki, David J Leehey, Vinod K Bansal, Linda Brubaker, Robert Flanigan, Julia Koval, Anuradha Wadhwa, Michael J Zilliox
PURPOSE: To examine the characteristics of the midstream urine microbiome in adults with stage 3-5 non-dialysis-dependent chronic kidney disease (CKD). METHODS: Patients with non-dialysis-dependent CKD (estimated glomerular filtration rate [eGFR] < 60 ml/min/1.73 m2 ) and diuretic use were recruited from outpatient nephrology clinics. Midstream voided urine specimens were collected using the clean-catch method. The bacterial composition was determined by sequencing the hypervariable (V4) region of the bacterial 16S ribosomal RNA gene...
April 12, 2018: International Urology and Nephrology
https://www.readbyqxmd.com/read/29642940/multi-cohort-analysis-of-colorectal-cancer-metagenome-identified-altered-bacteria-across-populations-and-universal-bacterial-markers
#13
Zhenwei Dai, Olabisi Oluwabukola Coker, Geicho Nakatsu, William K K Wu, Liuyang Zhao, Zigui Chen, Francis K L Chan, Karsten Kristiansen, Joseph J Y Sung, Sunny Hei Wong, Jun Yu
BACKGROUND: Alterations of gut microbiota are associated with colorectal cancer (CRC) in different populations and several bacterial species were found to contribute to the tumorigenesis. The potential use of gut microbes as markers for early diagnosis has also been reported. However, cohort specific noises may distort the structure of microbial dysbiosis in CRC and lead to inconsistent results among studies. In this regard, our study targeted at exploring changes in gut microbiota that are universal across populations at species level...
April 11, 2018: Microbiome
https://www.readbyqxmd.com/read/29642831/-identification-of-anaerobic-gram-negative-bacilli-isolated-from-various-clinical-specimens-and-determination-of-antibiotic-resistance-profiles-with-e-test-methods
#14
Cengiz Demir, Recep Keşli
The aim of this study was to identify gram-negative anaerobic bacilli isolated from various clinical specimens that were obtained from patients with suspected anaerobic infections and to determine the antibiotic resistance profiles by using the antibiotic concentration gradient method. The study was performed in Afyon Kocatepe University Ahmet Necdet Sezer Research and Practice Hospital, Medical Microbiology Laboratory between 1 November 2014 and 30 October 2015. Two hundred and seventyeight clinical specimens accepted for anaerobic culture were enrolled in the study...
January 2018: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29626219/smoking-and-the-intestinal-microbiome
#15
REVIEW
Ziv Savin, Shaye Kivity, Hagith Yonath, Shoenfeld Yehuda
Studies are emerging alluding to the role of intestinal microbiome in the pathogenesis of diseases. Intestinal microbiome is susceptible to the influence of environmental factors such as smoking, and recent studies have indicated microbiome alterations in smokers. The aim of the study was to review the literature regarding the impact of smoking on the intestinal microbiome. A literature review of publications in PUBMED was performed using combinations of the terms "Intestinal/Gut/Gastrointestinal/Colonic" with "Microbiome/Microbiota/Microbial/Flora" and "Smoking/Smoker/Tobacco"...
April 6, 2018: Archives of Microbiology
https://www.readbyqxmd.com/read/29624599/a-snapshot-of-gut-microbiota-of-an-adult-urban-population-from-western-region-of-india
#16
Disha Tandon, Mohammed Monzoorul Haque, Saravanan R, Shafiq Shaikh, Sriram P, Ashok Kumar Dubey, Sharmila S Mande
The human gut microbiome contributes to a broad range of biochemical and metabolic functions that directly or indirectly affect human physiology. Several recent studies have indicated that factors like age, geographical location, genetic makeup, and individual health status significantly influence the diversity, stability, and resilience of the gut microbiome. Of the mentioned factors, geographical location (and related dietary/socio-economic context) appears to explain a significant portion of microbiome variation observed in various previously conducted base-line studies on human gut microbiome...
2018: PloS One
https://www.readbyqxmd.com/read/29622802/design-and-assessment-of-engineered-crispr-cpf1-and-its-use-for-genome-editing
#17
Bin Li, Chunxi Zeng, Yizhou Dong
Cpf1, a CRISPR endonuclease discovered in Prevotella and Francisella 1 bacteria, offers an alternative platform for CRISPR-based genome editing beyond the commonly used CRISPR-Cas9 system originally discovered in Streptococcus pyogenes. This protocol enables the design of engineered CRISPR-Cpf1 components, both CRISPR RNAs (crRNAs) to guide the endonuclease and Cpf1 mRNAs to express the endonuclease protein, and provides experimental procedures for effective genome editing using this system. We also describe quantification of genome-editing activity and off-target effects of the engineered CRISPR-Cpf1 in human cell lines using both T7 endonuclease I (T7E1) assay and targeted deep sequencing...
May 2018: Nature Protocols
https://www.readbyqxmd.com/read/29619908/prevalence-of-sexually-transmitted-diseases-in-asymptomatic-renal-transplant-recipients
#18
Mehmet Sarier, Nevgun Sepin Ozen, Hicran Guler, Ibrahim Duman, Yücel Yüksel, Sabri Tekin, Asuman Havva Yavuz, Levent Yucetin, Mine Erdogan Yilmaz
OBJECTIVES: Sexually transmitted diseases, which may be asymptomatic, have the potential to cause serious health problems in renal transplant recipients. The aim of this study was to determine the prevalence of sexually transmitted diseases in sexually active asymptomatic renal transplant patients by using real-time multiplex polymerase chain reaction assays. MATERIALS AND METHODS: This prospective controlled study was conducted between November 2016 and January 2017 in our hospital...
April 4, 2018: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/29617868/responses-in-the-rumen-microbiome-of-bos-taurus-and-indicus-steers-fed-a-low-quality-rice-straw-diet-and-supplemented-protein
#19
E A Latham, K K Weldon, T A Wickersham, J A Coverdale, W E Pinchak
Bos indicus typically perform better than Bos taurus when consuming a low-quality diet; however, the response to supplementation is generally greater in B. taurus. The underlying mechanisms supporting these responses have not been fully elucidated. Characterization of differences in rumen prokaryotic populations and their functional role in the two subspecies may provide additional insight. Ten cannulated steers (5 Angus and 5 Brahman) were used in concurrent 5 × 5 Latin squares. Animals were offered ad libitum access to rice straw (4...
April 3, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29596446/gut-microbiota-varies-by-opioid-use-circulating-leptin-and-oxytocin-in-african-american-men-with-diabetes-and-high-burden-of-chronic-disease
#20
Elena Barengolts, Stefan J Green, Yuval Eisenberg, Arfana Akbar, Bharathi Reddivari, Brian T Layden, Lara Dugas, George Chlipala
OBJECTIVE: The gut microbiota is known to be related to type 2 diabetes (T2D), psychiatric conditions, and opioid use. In this study, we tested the hypothesis that variability in gut microbiota in T2D is associated with psycho-metabolic health. METHODS: A cross-sectional study was conducted among African American men (AAM) (n = 99) that were outpatients at a Chicago VA Medical Center. The main outcome measures included fecal microbiota ecology (by 16S rRNA gene sequencing), psychiatric disorders including opioid use, and circulating leptin and oxytocin as representative hormone biomarkers for obesity and psychological pro-social behavior...
2018: PloS One
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