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https://www.readbyqxmd.com/read/28440740/biomarkers-and-bacteria-around-implants-and-natural-teeth-in-the-same-individuals
#1
Önder Gürlek, Pınar Gümüş, Christopher J Nile, David F Lappin, Nurcan Buduneli
AIM: This cross-sectional study assessed cytokine levels in peri-implant crevicular fluid (PICF)/ gingival crevicular fluid (GCF) and a selection of subgingival/submucosal plaque bacteria from clinically healthy or diseased sites in the same individuals. MATERIAL AND METHODS: Samples from 97 implants/teeth (58 implants: 19 healthy, 20 mucositis, 19 peri-implantitis; 39 natural teeth: 19 healthy, 12 gingivitis, 8 periodontitis) in 15 systemically healthy patients were investigated by immunoassay, real-time PCR...
April 25, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28438498/performance-of-maldi-tof-ms-for-identification-of-oral-prevotella-species
#2
Mervi Gürsoy, Inka Harju, Jaakko Matomäki, Anne Bryk, Eija Könönen
During the past decade, the clinically relevant genus Prevotella has expanded considerably. Prevotella species can be isolated from nearly all types of oral infections but also from various non-oral infections. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been introduced in clinical microbiology laboratories as a convenient method for identifying bacterial isolates from clinical specimens. Here we tested the diagnostic accuracy of a total of 123 oral Prevotella isolates, selected based on their biochemical profile, by Bruker MALDI-TOF MS...
April 21, 2017: Anaerobe
https://www.readbyqxmd.com/read/28435379/shifts-of-subgingival-bacterial-population-after-nonsurgical-and-pharmacological-therapy-of-localized-aggressive-periodontitis-followed-for-1-year-by-ion-torrent-pgm-platform
#3
Giuseppina Campisciano, Annamaria Toschetti, Manola Comar, Rosanna Di Taranto, Federico Berton, Claudio Stacchi
The possibility of targeting the hypervariable region V3 of the 16S rRNA gene using Ion Torrent Personal Genome Machine (PGM) could provide a complete analysis of subgingival plaque samples, potentially able to identify microbiological species missed by culture-based methods. A 16-year-old female smoker patient, affected by localized aggressive periodontitis, underwent a full-mouth disinfection protocol and was inserted in a 3-month recall program. Microbiological samples were collected at baseline and at 30, 100, 365 days follow-up and analyzed by Ion Torrent PGM...
January 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28434745/hot-topic-16s-rrna-gene-sequencing-reveals-the-microbiome-of-the-virgin-and-pregnant-bovine-uterus
#4
S G Moore, A C Ericsson, S E Poock, P Melendez, M C Lucy
We tested the hypothesis that the uterus of virgin heifers and pregnant cows possessed a resident microbiome by 16S rRNA gene sequencing of the virgin and pregnant bovine uterus. The endometrium of 10 virgin heifers in estrus and the amniotic fluid, placentome, intercotyledonary placenta, cervical lumen, and external cervix surface (control) of 5 pregnant cows were sampled using aseptic techniques. The DNA was extracted, the V4 hypervariable region of the 16S rRNA gene was amplified, and amplicons were sequenced using Illumina MiSeq technology (Illumina Inc...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28434085/exploring-the-oral-microflora-of-preschool-children
#5
Wen Ren, Qun Zhang, Xuenan Liu, Shuguo Zheng, Lili Ma, Feng Chen, Tao Xu, Baohua Xu
The oral cavity is one of the most important and complicated habitats in our body and supports diverse microbial communities. In this study, we aimed to determine the bacterial diversity and composition of various oral micro-niches. Samples were collected from supragingival plaque, saliva, and tongue coating from 10 preschool children (30 samples total). 16S rRNA gene pyrosequencing dataset generated 314,639 clean reads with an average of 10,488 ± 2,787 reads per sample. The phyla Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Fusobacteria were predominant, accounting for more than 90% of the total sequences...
April 22, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28433670/evaluation-of-the-routine-antimicrobial-susceptibility-testing-results-of-clinically-significant-anaerobic-bacteria-in-a-slovenian-tertiary-care-hospital-in-2015
#6
Samo Jeverica, Urša Kolenc, Manica Mueller-Premru, Lea Papst
The aim of our study was to determined antimicrobial susceptibility profiles of 2673 clinically significant anaerobic bacteria belonging to the major genera, isolated in 2015 in a large tertiary-care hospital in Slovenia. The species identification was performed by MALDI-TOF mass spectrometry. Antimicrobial susceptibility was determined immediately at the isolation of the strains against: penicillin, co-amoxiclav, imipenem, clindamycin and metronidazole, using gradient diffusion methodology and EUCAST breakpoints...
April 19, 2017: Anaerobe
https://www.readbyqxmd.com/read/28432623/performance-of-cstr-egsb-sbr-system-for-treating-sulfate-rich-cellulosic-ethanol-wastewater-and-microbial-community-analysis
#7
Lili Shan, Zhaohan Zhang, Yanling Yu, John Justo Ambuchi, Yujie Feng
Performance and microbial community composition were evaluated in a two-phase anaerobic and aerobic system treating sulfate-rich cellulosic ethanol wastewater (CEW). The system was operated at five different chemical oxygen demand (COD)/SO4(2-) ratios (63.8, 26.3, 17.8, 13.7, and 10.7). Stable performance was obtained for total COD removal efficiency (94.5%), sulfate removal (89.3%), and methane production rate (11.5 L/day) at an organic loading rate of 32.4 kg COD/(m(3)·day). The acidogenic reactor made a positive contribution to net VFAs production (2318...
April 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28431945/in-vitro-fermentation-behaviors-of-fucosylated-chondroitin-sulfate-from-pearsonothuria-graeffei-by-human-gut-microflora
#8
Chao-Yang Wei, Ning-Bo Liao, Yu Zhang, Xing-Qian Ye, Shan Li, Ya-Qin Hu, Dong-Hong Liu, Robert J Linhardt, Xin Wang, Shi-Guo Chen
A fucosylated chondroitin sulfate (FCS-pg) with highly repeated structure from Pearsonothuria graeffei was subjected to a in vitro fermentation model to investigate its fermentability and effects on human gut microflora. High performance liquid chromatography (HPLC) measurement found FCS-pg can be fermented to short chain fatty acids (SCFAs) by gut microflora from partial human fecal samples. 16S rRNA gene-based polymerase chain reaction-based denaturing gradient gel electrophoresis (PCR-DGGE) profiling and real-time quantitative PCR analysis showed that FCS-pg mainly increased the proportions of Clostridium cluster XI, Bacteriodes prevotella group, Bifidobacterium genus, Clostridium cluster I and Clostridium cluster XIVab, whereas the numbers of the Enterobacteriaceae and Lactobacillus decreased...
April 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28429209/analysis-of-gut-microbiota-in-patients-with-parkinson-s-disease
#9
V A Petrov, I V Saltykova, I A Zhukova, V M Alifirova, N G Zhukova, Yu B Dorofeeva, A V Tyakht, B A Kovarsky, D G Alekseev, E S Kostryukova, Yu S Mironova, O P Izhboldina, M A Nikitina, T V Perevozchikova, E A Fait, V V Babenko, M T Vakhitova, V M Govorun, A E Sazonov
Gut microbiota of patients with Parkinson's disease and healthy volunteers was analyzed by the method of high throughput 16S rRNA sequencing of bacterial genomes. In patients with Parkinson's diseases, changes in the content of 9 genera and 15 species of microorganisms were revealed: reduced content of Dorea, Bacteroides, Prevotella, Faecalibacterium, Bacteroides massiliensis, Stoquefichus massiliensis, Bacteroides coprocola, Blautia glucerasea, Dorea longicatena, Bacteroides dorei, Bacteroides plebeus, Prevotella copri, Coprococcus eutactus, and Ruminococcus callidus, and increased content of Christensenella, Catabacter, Lactobacillus, Oscillospira, Bifidobacterium, Christensenella minuta, Catabacter hongkongensis, Lactobacillus mucosae, Ruminococcus bromii, and Papillibacter cinnamivorans...
April 20, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28428272/hur-small-molecule-inhibitor-elicits-differential-effects-in-adenomatosis-polyposis-and-colorectal-carcinogenesis
#10
Michaela Lang, David Berry, Katharina Passecker, Ildiko Mesteri, Sabin Bhuju, Florian Ebner, Vitaly Sedlyarov, Rayko Evstatiev, Kyle Dammann, Alexander Loy, Orest Kuzyk, Pavel Kovarik, Vineeta Khare, Martin Beibel, Guglielmo Roma, Nicole Meisner-Kober, Christoph Gasche
HuR is an RNA-binding protein implicated in immune homeostasis and various cancers, including colorectal cancer. HuR binding to AU-rich elements within the 3' untranslated region of mRNAs encoding oncogenes, growth factors, and various cytokines leads message stability and translation. In this study, we evaluated HuR as a small-molecule target for preventing colorectal cancer in high-risk groups such as those with familial adenomatosis polyposis (FAP) or inflammatory bowel disease (IBD). In human specimens, levels of cytoplasmic HuR were increased in colonic epithelial cells from patients with IBD, IBD-cancer, FAP-adenoma, and colorectal cancer, but not in patients with IBD-dysplasia...
February 20, 2017: Cancer Research
https://www.readbyqxmd.com/read/28425133/fecal-microbiota-signatures-of-adult-patients-with-different-types-of-short-bowel-syndrome
#11
Yuhua Huang, Feilong Guo, Yousheng Li, Jian Wang, Jieshou Li
BACKGROUND AND AIM: Short bowel syndrome (SBS) is a common cause of intestinal failure and can be divided into three types depending on intestinal anatomy. Gut dysbiosis has been observed in pediatric SBS patients and is associated with impaired outcome. Little is known about the changes in gut microbiota of adult SBS patients. Therefore, we aim to characterize the fecal microbiota of adult patients with different types of SBS. METHODS: Fifteen fecal samples from healthy controls and adult patients with type II or type III SBS were collected (five in each group)...
April 19, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28414794/intestinal-extra-intestinal-and-systemic-sequelae-of-toxoplasma-gondii-induced-acute-ileitis-in-mice-harboring-a-human-gut-microbiota
#12
Eliane von Klitzing, Ira Ekmekciu, Anja A Kühl, Stefan Bereswill, Markus M Heimesaat
BACKGROUND: Within seven days following peroral high dose infection with Toxoplasma gondii susceptible conventionally colonized mice develop acute ileitis due to an underlying T helper cell (Th) -1 type immunopathology. We here addressed whether mice harboring a human intestinal microbiota developed intestinal, extra-intestinal and systemic sequelae upon ileitis induction. METHODOLOGY/PRINCIPAL FINDINGS: Secondary abiotic mice were generated by broad-spectrum antibiotic treatment and associated with a complex human intestinal microbiota following peroral fecal microbiota transplantation...
2017: PloS One
https://www.readbyqxmd.com/read/28413200/the-oral-microbiome-community-variations-associated-with-normal-potentially-malignant-disorders-and-malignant-lesions-of-the-oral-cavity
#13
S F Mok, C Karuthan, Y K Cheah, W C Ngeow, Z Rosnah, S F Yap, H K A Ong
The human oral microbiome has been known to show strong association with various oral diseases including oral cancer. This study attempts to characterize the community variations between normal, oral potentially malignant disorders (OPMD) and cancer associated microbiota using 16S rDNA sequencing. Swab samples were collected from three groups (normal, OPMD and oral cancer) with nine subjects from each group. Bacteria genomic DNA was isolated in which full length 16S rDNA were amplified and used for cloned library sequencing...
April 2017: Malaysian Journal of Pathology
https://www.readbyqxmd.com/read/28406692/effect-of-pre-and-post-weaning-high-fat-dietary-manipulation-on-intestinal-microflora-and-alkaline-phosphatase-activity-in-male-rats
#14
Z Šefčíková, D Bujňáková
We investigated the impact of a high-fat (HF) diet during pre- and post-weaning periods on the intestinal microbiota and alkaline phosphatase (AP) activity in male rats. Nutrition from birth was influenced by feeding rat dams with either a standard or HF diet. After weaning male pups nursed by control dams continued on a standard diet (CC) or HF diet (C->HF), while offspring nursed by HF dams continued on HF diet (HF) or standard diet (HF->C). The numbers of Bacteriodes/Prevotella (BAC) and Lactobacillus/Enterococcus (LAB) in the gut were determined by FISH technique...
April 12, 2017: Physiological Research
https://www.readbyqxmd.com/read/28392784/structure-and-function-of-the-fecal-microbiota-in-diarrheic-neonatal-piglets
#15
Qiaoli Yang, Xiaoyu Huang, Shengguo Zhao, Wenyang Sun, Zunqiang Yan, Pengfei Wang, Shenggui Li, Wangzhou Huang, Shengwei Zhang, Lixia Liu, Shuangbao Gun
Diarrhea is a leading cause of increased mortality in neonatal and young piglets. Aberration of the gut microbiota is one important factor in the etiology of piglet diarrhea. However, information regarding the structure and function of the gut microbiome in diarrheic neonatal piglets is limited. To investigate the composition and functional potential of the fecal microbiota in neonatal piglets, we performed 16S rRNA gene sequencing on 20 fecal samples from diarrheic piglets and healthy controls, and metagenomics sequencing on a subset of six samples...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28390722/factors-influencing-ruminal-bacterial-community-diversity-and-composition-and-microbial-fibrolytic-enzyme-abundance-in-lactating-dairy-cows-with-a-focus-on-the-role-of-active-dry-yeast
#16
Ousama AlZahal, Fuyong Li, Le Luo Guan, Nicola D Walker, Brian W McBride
The objective of the current study was to employ a DNA-based sequencing technology to study the effect of active dry yeast (ADY) supplementation, diet type, and sample location within the rumen on rumen bacterial community diversity and composition, and to use an RNA-based method to study the effect of ADY supplementation on rumen microbial metabolism during high-grain feeding (HG). Our previous report demonstrated that the supplementation of lactating dairy cows with ADY attenuated the effect of subacute ruminal acidosis...
April 5, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28386896/antimicrobial-activity-of-aqueous-extracts-from-four-plants-on-bacterial-isolates-from-periodontitis-patients
#17
Leila Arbia, Nassima Chikhi-Chorfi, Ilhem Betatache, Chuong Pham-Huy, Selma Zenia, Nabil Mameri, Nadjib Drouiche, Hakim Lounici
Four aqueous extracts of different plant organs are the following: Artemisia herba-alba, Opuntia ficus-indica, Camellia sinensis and Phlomis crinita were evaluated against two bacterial strains: Porphyromonas gingivalis and Prevotella intermedia, which are implicated in periodontal diseases. By using a disc method, these plant extracts demonstrated powerful bacterial activity against these Gram-negative strains. The minimum inhibitory concentration values of the four plant extracts varied between 0.03 and 590...
April 6, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28386472/comprehensive-survey-of-intestinal-microbiota-changes-in-offspring-of-human-microbiota-associated-mice
#18
Eliane von Klitzing, Fulya Öz, Ira Ekmekciu, Ulrike Escher, Stefan Bereswill, Markus M Heimesaat
Secondary abiotic mice generated by broad-spectrum antibiotic treatment provide a valuable tool for association studies with microbiota derived from different vertebrate hosts. We here generated human microbiota-associated (hma) mice by human fecal microbiota transplantation of secondary abiotic mice and performed a comprehensive survey of the intestinal microbiota dynamics in offspring of hma mice over 18 weeks following weaning as compared to their mothers applying both cultural and molecular methods. Mice were maintained under standard hygienic conditions with open cages, handled under aseptic conditions, and fed autoclaved chow and water...
March 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28383789/salivary-microbiome-of-an-urban-indian-cohort-and-patterns-linked-to-subclinical-inflammation
#19
Aneesha Acharya, Yuki Chan, Supriya Kheur, Mohit Kheur, D Gopalakrishnan, R M Watt, Nikos Mattheos
OBJECTIVE: To profile salivary microbiomes of an urban-living, healthy Indian cohort and explore associations with proinflammatory status. METHODS: 51 clinically healthy Indian subjects' salivary microbiomes were analyzed using 16S rRNA Illumina MiSeq sequencing. Community distribution was compared with salivary data from the Human Microbiome Project (HMP). Indian subjects were clustered using microbiome based 'partitioning along medoids' (PAM) and relationships of interleukin 1 beta levels with community composition were analyzed...
April 6, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28383606/generalized-aggressive-periodontitis-microbiological-composition-and-clinical-parameters-in-non-surgical-therapy
#20
María M Usin, Sandra M Tabares, Julieta Menso, Estela R de Albera, Adela Sembaj
The aim of this study was to determine the variations in periodontal parameters and microbiological composition in periodontal pockets at the baseline and 3 and 6 months post treatmentin patients with Generalized Aggressive Periodontitis(GAP) undergoing non surgical periodontal treatment combined with chlorhexidine and systemic antibiotics. Medical and dental history was taken from 10 subjects, average age 30.6±2.7 years, diagnosed with GAP. A non surgical periodontal treatment combined with 0.12% chlorhexidine, 875 mg amoxicillin and 500 mg metronidazole every 12 hours for ten days was conducted...
December 2016: Acta Odontológica Latinoamericana: AOL
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