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https://www.readbyqxmd.com/read/28338745/circadian-oscillations-of-microbial-and-functional-composition-in-the-human-salivary-microbiome
#1
Lena Takayasu, Wataru Suda, Kageyasu Takanashi, Erica Iioka, Rina Kurokawa, Chie Shindo, Yasue Hattori, Naoko Yamashita, Suguru Nishijima, Kenshiro Oshima, Masahira Hattori
The human microbiomes across the body evidently interact with various signals in response to biogeographical physiological conditions. To understand such interactions in detail, we investigated how the salivary microbiome in the oral cavity would be regulated by host-related signals. Here, we show that the microbial abundance and gene participating in keeping the human salivary microbiome exhibit global circadian rhythm. Analysis of the 16S rRNA sequences of salivary microbial samples of six healthy adults collected at 4-h intervals for three days revealed that the microbial genera accounting for 68...
February 23, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28331805/prevotella-osteomyelitis-after-dental-capping-procedure
#2
Alexander Williams, Thomas Kerkering
We present a 49 year old man who presented to the emergency department with severe lower back pain of 5 days duration. One week prior he had been diagnosed with a right psoas muscle abscess and was discharged with a 28 day course of moxifloxacin after the preliminary culture from the psoas grew an anaerobic organism. MRI of the lumbar spine showed marrow edema in the L2 body, occupying most of the anterior two thirds of the body, with cortical erosion at the anteroinferior aspect of L1. Blood cultures were negative and bone biopsy of L2 showed neutrophilic invasion and reactive changes...
2017: IDCases
https://www.readbyqxmd.com/read/28326152/candida-albicans-alters-the-bacterial-microbiome-of-early-in-vitro-oral-biofilms
#3
M M Janus, W Crielaard, C M C Volgenant, M H van der Veen, B W Brandt, B P Krom
The yeast Candida albicans is an oral commensal microorganism, occurring in the oral cavity of 50-70% of healthy individuals. Its effect on oral ecology has mostly been studied using dual-species models, which disregards the complex nature of oral biofilms. The aim of this study was to culture C. albicans in a complex model to study its effect on oral biofilms. Biofilms, inoculated using pooled stimulated saliva with or without addition of C. albicans, were grown under anaerobic, aerobic, or aerobic +5% CO2 conditions...
2017: Journal of Oral Microbiology
https://www.readbyqxmd.com/read/28320468/subgingival-microbiota-dysbiosis-in-systemic-lupus-erythematosus-association-with-periodontal-status
#4
Jôice Dias Corrêa, Débora Cerqueira Calderaro, Gilda Aparecida Ferreira, Santuza Maria Souza Mendonça, Gabriel R Fernandes, E Xiao, Antônio Lúcio Teixeira, Eugene J Leys, Dana T Graves, Tarcília Aparecida Silva
BACKGROUND: Periodontitis results from the interaction between a subgingival biofilm and host immune response. Changes in biofilm composition are thought to disrupt homeostasis between the host and subgingival bacteria resulting in periodontal damage. Chronic systemic inflammatory disorders have been shown to affect the subgingival microbiota and clinical periodontal status. However, this relationship has not been examined in subjects with systemic lupus erythematosus (SLE). The objective of our study was to investigate the influence of SLE on the subgingival microbiota and its connection with periodontal disease and SLE activity...
March 20, 2017: Microbiome
https://www.readbyqxmd.com/read/28316961/acute-delayed-or-late-infection-of-revision-total-hip-arthroplasty-treated-with-debridement-antibiotic-loaded-cement-beads-and-retention-of-the-prosthesis
#5
Jun-Dong Chang, In-Sung Kim, Sang-Soo Lee, Je-Hyun Yoo
PURPOSE: The treatment of infected revision total hip arthroplasty (THA) is very challenging due to retained revision prosthesis, poor bone stock and soft tissue condition derived from previous revision surgeries, and comorbidities. The purpose of this study was to investigate the effectiveness and short-term outcomes of aggressive debridement and use of antibiotic-loaded cement beads with retention of the prosthesis for acute delayed or late infection of revision THAs. MATERIALS AND METHODS: Ten consecutive patients with symptoms or signs of less than one-week evolution and well-fixed prostheses, were treated with this procedure and a postoperative course of organism-specific antibiotics for a minimum of 6 weeks...
March 2017: Hip & Pelvis
https://www.readbyqxmd.com/read/28316574/alterations-of-urinary-microbiota-in-type-2-diabetes-mellitus-with-hypertension-and-or-hyperlipidemia
#6
Fengping Liu, Zongxin Ling, Yonghong Xiao, Qing Yang, Baohong Wang, Li Zheng, Ping Jiang, Lanjuan Li, Wei Wang
Evidence shows urine specimens from different women have different populations of bacteria. The co-occurrence of hypertension and hyperlipidemia in those with diabetes may alter the composition of urine and the microenviroment of the bladder in which bacteria live. The aim of this study was to characterize the urinary microbiota in women with type 2 diabetes mellitus only and those with diabetes plus hypertension and/or hyperlipidemia, and to explore whether the composition of the urinary microbiota is affected by fasting blood glucose, blood pressure, and blood lipids...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28301146/effect-of-whole-grain-qingke-tibetan-hordeum-vulgare-l-zangqing-320-on-the-serum-lipid-levels-and-intestinal-microbiota-of-rats-under-high-fat-diet
#7
Xuejuan Xia, Guannan Li, Yongbo Ding, Tingyuan Ren, Jiong Zheng, Jianquan Kan
This study investigated the hypolipidemic effect of whole grain Qingke (WGQ) and its influence on intestinal microbiota. Changes in the serum lipid, intestinal environment, and microbiota of Sprague-Dawley rats fed high-fat diets supplemented with different doses of WGQ were determined. Results showed that high doses of WGQ significantly decreased (P < 0.05) the Lee's index, serum total cholesterol, low-density lipoprotein cholesterol, and non-high-density lipoprotein cholesterol levels whereas they increased the body weight of the rats...
March 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28298816/effect-of-gallium-arsenic-laser-on-photosensitized-periodontopathic-anaerobic-organisms-an-in-vitro-study
#8
Setu Mathur, Shaila Veerappa Kothiwale, Buddhi Prakash Nag, Tanu Mathur, Ashoka Bhansali, Rohit Kumar Khatri
BACKGROUND: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser...
July 2016: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/28293225/bacterial-composition-genotoxicity-and-cytotoxicity-of-fecal-samples-from-individuals-consuming-omnivorous-or-vegetarian-diets
#9
Ermanno Federici, Roberta Prete, Camilla Lazzi, Nicoletta Pellegrini, Massimo Moretti, Aldo Corsetti, Giovanni Cenci
This study analyzes the composition of viable fecal bacteria and gut toxicology biomarkers of 29 healthy volunteers, who followed omnivorous, lacto-ovo-vegetarian, or vegan diets. In particular, the research was focused on the prevalence of some representative viable bacteria from the four dominant phyla (Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria) commonly present in human feces, in order to evaluate the relationship between microorganisms selected by the habitual dietary patterns and the potential risk due to fecal water (FW) genotoxicity and cytotoxicity, considered as biomarkers for cancer risk and protective food activity...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28285654/gut-microbiota-interactions-with-the-immunomodulatory-role-of-vitamin-d-in-normal-individuals
#10
Renata V Luthold, Gabriel R Fernandes, Ana Carolina Franco-de-Moraes, Luciana G D Folchetti, Sandra Roberta G Ferreira
BACKGROUND: Due to immunomodulatory properties, vitamin D status has been implicated in several diseases beyond the skeletal disorders. There is evidence that its deficiency deteriorates the gut barrier favoring translocation of endotoxins into the circulation and systemic inflammation. Few studies investigated whether the relationship between vitamin D status and metabolic disorders would be mediated by the gut microbiota composition. OBJECTIVE: We examined the association between vitamin D intake and circulating levels of 25(OH)D with gut microbiota composition, inflammatory markers and biochemical profile in healthy individuals...
April 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28282386/strong-incidence-of-pseudomonas-aeruginosa-on-bacterial-rrs-and-its-genetic-structures-of-cystic-fibrosis-sputa
#11
Laurence Pages-Monteiro, Romain Marti, Carine Commun, Nolwenn Alliot, Claire Bardel, Helene Meugnier, Michele Perouse-de-Montclos, Philippe Reix, Isabelle Durieu, Stephane Durupt, Francois Vandenesch, Jean Freney, Benoit Cournoyer, Anne Doleans-Jordheim
Cystic fibrosis (CF) lungs harbor a complex community of interacting microbes, including pathogens like Pseudomonas aeruginosa. Meta-taxogenomic analysis based on V5-V6 rrs PCR products of 52 P. aeruginosa-positive (Pp) and 52 P. aeruginosa-negative (Pn) pooled DNA extracts from CF sputa suggested positive associations between P. aeruginosa and Stenotrophomonas and Prevotella, but negative ones with Haemophilus, Neisseria and Burkholderia. Internal Transcribed Spacer analyses (RISA) from individual DNA extracts identified three significant genetic structures within the CF cohorts, and indicated an impact of P...
2017: PloS One
https://www.readbyqxmd.com/read/28280715/enterotype-may-drive-the-dietary-associated-cardiometabolic-risk-factors
#12
Ana C F de Moraes, Gabriel R Fernandes, Isis T da Silva, Bianca Almeida-Pititto, Everton P Gomes, Alexandre da Costa Pereira, Sandra R G Ferreira
Analyses of typical bacterial clusters in humans named enterotypes may facilitate understanding the host differences in the cardiometabolic profile. It stills unknown whether the three previously described enterotypes were present in populations living below the equator. We examined how the identification of enterotypes could be useful to explain the dietary associations with cardiometabolic risk factors in Brazilian subjects. In this cross-sectional study, a convenience sample of 268 adults (54.2% women) reported their dietary habits and had clinical and biological samples collected...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28279857/performance-of-two-blood-culture-systems-to-detect-anaerobic-bacteria-is-there-any-difference
#13
Manica Mueller-Premru, Samo Jeverica, Lea Papst, Elisabeth Nagy
We studied the performance characteristics of two blood culture (BC) bottles/systems, (i) BacT/ALERT-FN Plus/3D (bioMérieux, Marcy l'Étoile, France) and (ii) BACTEC-Lytic/9000 (Becton Dickinson, Sparks, USA) for detection of growth and time-to-positivity (TTP) against a balanced and diverse collection of anaerobic bacterial strains (n = 48) that included reference strains (n = 19) and clinical isolates (n = 29) of 32 species (15 Gram-negative and 17 Gram-positive) was tested. Standard suspension of bacteria was inoculated to each bottle in duplicates and incubated in the corresponding system...
March 6, 2017: Anaerobe
https://www.readbyqxmd.com/read/28276583/effect-of-photodynamic-therapy-in-combination-with-various-irrigation-protocols-on-an-endodontic-multispecies-biofilm-ex-vivo
#14
D Hoedke, C Enseleit, D Gruner, H Dommisch, S Schlafer, I Dige, K Bitter
AIM: To analyse the antibacterial effect of photodynamic therapy (PDT) in combination with various irrigation protocols on a multispecies biofilm in root canals ex vivo. METHODOLOGY: A total of 160 extracted human single-rooted teeth were divided into four groups (n = 40). In group (G) 1, root canals were instrumented up to size 60 (control group), whereas in G 2 - 4 canals were enlarged up to size 40. All root canals were inoculated with a multispecies biofilm (Enterococcus faecalis, Streptococcus oralis, Prevotella intermedia) for five days...
March 9, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28274501/microbiological-and-ammp-8-findings-depending-on-peri-implant-disease-in-patients-undergoing-supportive-implant-therapy
#15
Dirk Ziebolz, Gerhard Schmalz, Daniel Gollasch, Peter Eickholz, Sven Rinke
The aim of this study was to evaluate microbiological findings and aMMP-8 level of peri-implant mucositis (M) and peri-implantitis (P) in patients undergoing supportive implant therapy (SIT). Eighty-nine patients with 171 implants were included. The case definitions were as follows: M: PPD ≥4mm, BOP; P: PPD≥4mm, BOP, radiographic bone loss ≥3.5mm. Samples of peri-implant sulcular fluid (PISF) were taken from all peri-implant pockets at each implant to detect periodontal pathogens using PCR and aMMP-8 level with ELISA...
February 21, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28274127/-development-and-evaluation-of-a-new-selective-culture-medium-kbm-anaero-rs-gnr-for-detection-of-anaerobic-gram-negative-rods
#16
Taeko Narita, Kyohei Kato, Hiroki Hanaiwa, Tetsuhiro Harada, Yumiko Funashima, Makoto Akiwa, Jun-Ichiro Sekiguchi, Zenzo Nagasawa, Tsukuru Umemura
The laboratory culture methods for isolating drug-resistant pathogens has been the gold standard in medical microbiology, and play pivotal roles in the overall management of infectious diseases. Recently, several reports have emphasized the development of antibiotics-resistance among anaerobic gram-negative rods, especially Genus Bacteroides and Prevotella. Therefore, a selective culture method to detect these pathogens is needed. We developed here the new selective culture medium, termed "KBM Anaero RS-GNR," for detecting anaerobic Gram-negative rods...
March 22, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28266623/commensal-gut-bacteria-modulate-phosphorylation-dependent-ppar%C3%AE-transcriptional-activity-in-human-intestinal-epithelial-cells
#17
Malgorzata Nepelska, Tomas de Wouters, Elsa Jacouton, Fabienne Béguet-Crespel, Nicolas Lapaque, Joël Doré, Velmurugesan Arulampalam, Hervé M Blottière
In healthy subjects, the intestinal microbiota interacts with the host's epithelium, regulating gene expression to the benefit of both, host and microbiota. The underlying mechanisms remain poorly understood, however. Although many gut bacteria are not yet cultured, constantly growing culture collections have been established. We selected 57 representative commensal bacterial strains to study bacteria-host interactions, focusing on PPARγ, a key nuclear receptor in colonocytes linking metabolism and inflammation to the microbiota...
March 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28265505/key-bacterial-families-clostridiaceae-erysipelotrichaceae-and-bacteroidaceae-are-related-to-the-digestion-of-protein-and-energy-in-dogs
#18
Emma N Bermingham, Paul Maclean, David G Thomas, Nicholas J Cave, Wayne Young
BACKGROUND: Much of the recent research in companion animal nutrition has focussed on understanding the role of diet on faecal microbiota composition. To date, diet-induced changes in faecal microbiota observed in humans and rodents have been extrapolated to pets in spite of their very different dietary and metabolic requirements. This lack of direct evidence means that the mechanisms by which microbiota influences health in dogs are poorly understood. We hypothesised that changes in faecal microbiota correlate with physiological parameters including apparent macronutrient digestibility...
2017: PeerJ
https://www.readbyqxmd.com/read/28262674/effect-of-predatory-bacteria-on-the-gut-bacterial-microbiota-in-rats
#19
Kenneth Shatzkes, Chi Tang, Eric Singleton, Sean Shukla, Michael Zuena, Shilpi Gupta, Sonal Dharani, Joseph Rinaggio, Nancy D Connell, Daniel E Kadouri
Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are Gram-negative proteobacteria that are obligate predators of other Gram-negative bacteria and are considered potential alternatives to antibiotics. Most studies focusing on predatory bacteria have been performed in vitro, thus the effect of predatory bacteria on a live host, including the impact on the ecology of the native microbiota, has yet to be fully examined. In this study, intrarectal inoculations of Sprague-Dawley rats with predatory bacteria were performed...
March 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28261182/methane-production-in-dairy-cows-correlates-with-rumen-methanogenic-and-bacterial-community-structure
#20
Rebecca Danielsson, Johan Dicksved, Li Sun, Horacio Gonda, Bettina Müller, Anna Schnürer, Jan Bertilsson
Methane (CH4) is produced as an end product from feed fermentation in the rumen. Yield of CH4 varies between individuals despite identical feeding conditions. To get a better understanding of factors behind the individual variation, 73 dairy cows given the same feed but differing in CH4 emissions were investigated with focus on fiber digestion, fermentation end products and bacterial and archaeal composition. In total 21 cows (12 Holstein, 9 Swedish Red) identified as persistent low, medium or high CH4 emitters over a 3 month period were furthermore chosen for analysis of microbial community structure in rumen fluid...
2017: Frontiers in Microbiology
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