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https://www.readbyqxmd.com/read/29218037/wuji-wan-formula-ameliorates-diarrhea-and-disordered-colonic-motility-in-post-inflammation-irritable-bowel-syndrome-rats-by-modulating-the-gut-microbiota
#1
Ying Chen, Shuiming Xiao, Zipeng Gong, Xiaoxin Zhu, Qing Yang, Yujie Li, Shuangrong Gao, Yu Dong, Zhe Shi, Yajie Wang, Xiaogang Weng, Qi Li, Weiyan Cai, Weijie Qiang
Emerging evidence suggests that gut microbiota contribute to the treatment of post-inflammatory irritable bowel syndrome (PI-IBS). Our previous studies have demonstrated that a Chinese formula, Wuji Wan, has the ability to mitigate abdominal pain and diarrhea in PI-IBS rats. However, little is known about the underlying mechanism and whether the gut microbiota mediate the effect of Wuji Wan on PI-IBS. Thus, the aim of this study was to determine whether Wuji Wan mitigated PI-IBS by modifying the gut microbiota...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29214754/utility-of-conventional-culture-and-maldi-tof-ms-for-identification-of-microbial-communities-in-bronchoalveolar-lavage-fluid-in-comparison-with-the-gs-junior-next-generation-sequencing-system
#2
Ji Yeon Sung, Younjee Hwang, Mi Hwa Shin, Moo Suk Park, Sang Hoon Lee, Dongeun Yong, Kyungwon Lee
BACKGROUND: Diverse microbiota exist in the lower respiratory tract. Although next generation sequencing (NGS) is the most widely used microbiome analysis technique, it is difficult to implement NGS in clinical microbiology laboratories. Therefore, we evaluated the performance of conventional culture methods together with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying microbiota in bronchoalveolar lavage (BAL) fluid. METHODS: BAL fluid samples (n=27) were obtained from patients undergoing diagnostic bronchoscopy for lung mass evaluation...
March 2018: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/29209019/correlations-between-gut-microbiota-community-structures-of-tibetans-and-geography
#3
Daoliang Lan, Wenhui Ji, Baoshan Lin, Yabing Chen, Cai Huang, Xianrong Xiong, Mei Fu, Tserang Donko Mipam, Yi Ai, Bo Zeng, Yin Li, Zhixin Cai, Jiangjiang Zhu, Dawei Zhang, Jian Li
Microbial communities of human gut directly influence health and bear adaptive potential to different geography environment and lifestyles. However, knowledge about the influences of altitude and geography on the gut microbiota of Tibetans is currently limited. In this study, fecal microbiota from 208 Tibetans across six different locations were analyzed by MiSeq sequencing; these locations included Gannan, Gangcha, Tianzhu, Hongyuan, Lhasa and Nagqu, with altitudes above sea level ranging from 2800 m to 4500 m across the Tibetan plateau...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29204141/flos-lonicera-combined-with-metformin-ameliorates-hepatosteatosis-and-glucose-intolerance-in-association-with-gut-microbiota-modulation
#4
Na R Shin, Shambhunath Bose, Jing-Hua Wang, AbuZar Ansari, Soo-Kyoung Lim, Young-Won Chin, Han-Seok Choi, Hojun Kim
The gut microbiota is important in energy contribution, metabolism and immune modulation, and compositional disruption of the gut microbiota population is closely associated with chronic metabolic diseases like type 2 diabetes (T2D) and non-alcoholic fatty liver disease (NAFLD). Metformin (MET) and Flos Lonicera (FL) are common treatments for metabolic diseases in Western and Oriental medicinal fields. We evaluated the effect of treatment with FL and MET in combination on hepatosteatosis, glucose tolerance, and gut microbial composition...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29204120/alternation-of-gut-microbiota-in-patients-with-pulmonary-tuberculosis
#5
Mei Luo, Yong Liu, Pengfei Wu, Dong-Xia Luo, Qun Sun, Han Zheng, Richard Hu, Stephen J Pandol, Qing-Feng Li, Yuan-Ping Han, Yilan Zeng
One-third of the world's population has been infected with Mycobacterium tuberculosis (M. tuberculosis), a primary pathogen of the mammalian respiratory system, while about 10% of latent infections progress to active tuberculosis (TB), indicating that host and environmental factors may determine the outcomes such as infection clearance/persistence and treatment prognosis. The gut microbiota is essential for development of host immunity, defense, nutrition and metabolic homeostasis. Thus, the pattern of gut microbiota may contribute to M...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29201017/characterization-of-metagenomes-in-urban-aquatic-compartments-reveals-high-prevalence-of-clinically-relevant-antibiotic-resistance-genes-in-wastewaters
#6
Charmaine Ng, Martin Tay, Boonfei Tan, Thai-Hoang Le, Laurence Haller, Hongjie Chen, Tse H Koh, Timothy M S Barkham, Karina Y-H Gin
The dissemination of antimicrobial resistance (AMR) is an escalating problem and a threat to public health. Comparative metagenomics was used to investigate the occurrence of antibiotic resistant genes (ARGs) in wastewater and urban surface water environments in Singapore. Hospital and municipal wastewater (n = 6) were found to have higher diversity and average abundance of ARGs (303 ARG subtypes, 197,816 x/Gb) compared to treated wastewater effluent (n = 2, 58 ARG subtypes, 2,692 x/Gb) and surface water (n = 5, 35 subtypes, 7,985 x/Gb)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29199351/significant-changes-in-the-skin-microbiome-in-patients-with-chronic-plaque-psoriasis-after-treatment-with-narrowband-ultraviolet-b
#7
Malin Assarsson, Albert Duvetorp, Olaf Dienus, Jan Söderman, Oliver Seifert
Changes in the skin microbiome have been shown to promote cutaneous inflammation. The skin microbiome of patients with chronic plaque type psoriasis was analysed before and after treatment with narrowband ultraviolet B (UVB). Swab samples of the microbiome were taken from lesional and non-lesional skin of 26 patients. Microbiotas were characterized by sequencing 16S rRNA bacterial genes on the Illumina MiSeq platform. Lesional skin microbiome diversity correlated with psoriasis severity (measured with the Psoriasis Area and Severity Index; PASI)...
December 4, 2017: Acta Dermato-venereologica
https://www.readbyqxmd.com/read/29188819/colonic-bacteroides-are-positively-associated-with-trabecular-bone-structure-and-programmed-by-maternal-vitamin-d-in-male-but-not-female-offspring-in-an-obesogenic-environment
#8
C R Villa, A Taibi, J Chen, W E Ward, E M Comelli
BACKGROUND/OBJECTIVES: The gut microbiota is determined early in life, possibly including pregnancy. Pioneering data suggests vitamin D, a nutrient important for bone health, affects this microbiota. We found that high maternal vitamin D lowered circulating lipopolysaccharide, improved intestinal barrier and bone health in male but not female offspring in an obesogenic environment. This study determined if high maternal dietary vitamin D programs Bacteroides and Prevotella and whether this associates with bone mineral content, density and structure of male and female adult offspring fed an obesogenic diet...
November 30, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29187843/profiling-of-oral-microbiota-in-early-childhood-caries-using-single-molecule-real-time-sequencing
#9
Yuan Wang, Jie Zhang, Xi Chen, Wen Jiang, Sa Wang, Lei Xu, Yan Tu, Pei Zheng, Ying Wang, Xiaolong Lin, Hui Chen
Background: Alterations of oral microbiota are the main cause of the progression of caries. The goal of this study was to characterize the oral microbiota in childhood caries based on single-molecule real-time sequencing. Methods: A total of 21 preschoolers, aged 3-5 years old with severe early childhood caries, and 20 age-matched, caries-free children as controls were recruited. Saliva samples were collected, followed by DNA extraction, Pacbio sequencing, and phylogenetic analyses of the oral microbial communities...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29185932/effects-of-partial-replacement-of-maize-in-the-diet-with-crude-glycerin-and-or-soyabean-oil-on-ruminal-fermentation-and-microbial-population-in-nellore-steers
#10
Yury Tatiana Granja-Salcedo, Juliana Duarte Messana, Vinícius Carneiro de Souza, Ana Veronica Lino Dias, Luciano Takeshi Kishi, Lucas Rocha Rebelo, Telma Teresinha Berchielli
The objective of this study was to determine whether a combination of crude glycerin (CG) and soyabean oil (SO) could be used to partially replace maize in the diet of Nellore steers while maintaining optimum feed utilisation. Eight castrated Nellore steers fitted with ruminal and duodenal cannulas were used in a double 4×4 Latin square design balanced for residual effects, in a factorial arrangement (A×B), when factor A corresponded to the provision of SO, and factor B to the provision of CG. Steers feed SO and CG showed similar DM intake, DM, organic matter and neutral-detergent fibre digestibility to that of steers fed diets without oil and without glycerine (P>0·05)...
November 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/29180323/-pathogen-analysis-in-patients-with-diabetic-foot-osteomyelitis-using-16s-rrna-high-throughput-sequencing
#11
Ping Hu, Meng-Chen Zou, Ying Cao, Yan-Ling Pan, Xiang-Rong Luo, Ya Jiang, Yao-Ming Xue, Fang Gao
OBJECTIVE: To analyze the characteristics of pathogenic microorganisms in the infected bone tissues in patients with diabetic foot osteomyelitis (DFO) using 16S rRNA high-throughput sequencing to facilitate rapid and accurate detection of pathogens and effective infection control. METHODS: Between September, 2016 and April, 2017, 16 patients with DFO were admitted in our department and infected bone specimens were obtained during debridement. The pathogenic microorganisms in the specimens were identified using both 16S rRNA high-throughput sequencing and automatic blood culture analyzer, and the characteristics of the microflora were analyzed based on 16S rRNA sequencing data in comparison with the results of blood culture...
November 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/29176714/analyses-of-gut-microbiota-and-plasma-bile-acids-enable-stratification-of-patients-for-antidiabetic-treatment
#12
Yanyun Gu, Xiaokai Wang, Junhua Li, Yifei Zhang, Huanzi Zhong, Ruixin Liu, Dongya Zhang, Qiang Feng, Xiaoyan Xie, Jie Hong, Huahui Ren, Wei Liu, Jing Ma, Qing Su, Hongmei Zhang, Jialin Yang, Xiaoling Wang, Xinjie Zhao, Weiqiong Gu, Yufang Bi, Yongde Peng, Xiaoqiang Xu, Huihua Xia, Fang Li, Xun Xu, Huanming Yang, Guowang Xu, Lise Madsen, Karsten Kristiansen, Guang Ning, Weiqing Wang
Antidiabetic medication may modulate the gut microbiota and thereby alter plasma and faecal bile acid (BA) composition, which may improve metabolic health. Here we show that treatment with Acarbose, but not Glipizide, increases the ratio between primary BAs and secondary BAs and plasma levels of unconjugated BAs in treatment-naive type 2 diabetes (T2D) patients, which may beneficially affect metabolism. Acarbose increases the relative abundances of Lactobacillus and Bifidobacterium in the gut microbiota and depletes Bacteroides, thereby changing the relative abundance of microbial genes involved in BA metabolism...
November 27, 2017: Nature Communications
https://www.readbyqxmd.com/read/29170280/analysis-of-fusobacterium-persistence-and-antibiotic-response-in-colorectal-cancer
#13
Susan Bullman, Chandra S Pedamallu, Ewa Sicinska, Thomas E Clancy, Xiaoyang Zhang, Diana Cai, Donna Neuberg, Katherine Huang, Fatima Guevara, Timothy Nelson, Otari Chipashvili, Timothy Hagan, Mark Walker, Aruna Ramachandran, Begoña Diosdado, Garazi Serna, Nuria Mulet, Stefania Landolfi, Santiago Ramon Y Cajal, Roberta Fasani, Andrew J Aguirre, Kimmie Ng, Elena Élez, Shuji Ogino, Josep Tabernero, Charles S Fuchs, William C Hahn, Paolo Nuciforo, Matthew Meyerson
Colorectal cancers comprise a complex mixture of malignant cells, non-transformed cells and microorganisms. Fusobacterium nucleatum is among the most prevalent bacterial species in colorectal cancer tissues. Here we show that colonization of human colorectal cancers with Fusobacterium and its associated microbiome, including Bacteroides, Selenomonas and Prevotella species, is maintained in distal metastases, demonstrating microbiome stability between paired primary-metastatic tumors. In situ hybridization analysis revealed that Fusobacterium is predominantly associated with cancer cells in the metastatic lesions...
November 23, 2017: Science
https://www.readbyqxmd.com/read/29167659/balancing-herbal-medicine-and-functional-food-for-prevention-and-treatment-of-cardiometabolic-diseases-through-modulating-gut-microbiota
#14
REVIEW
Ming Lyu, Yue-Fei Wang, Guan-Wei Fan, Xiao-Ying Wang, Shuang-Yong Xu, Yan Zhu
It has become apparent that gut microbiota is closely associated with cardiometabolic diseases (CMDs), and alteration in microbiome compositions is also linked to the host environment. Next generation sequencing (NGS) has facilitated in-depth studies on the effects of herbal medicine and functional food on gut microbiota. Both herbal medicine and functional food contain fiber, polyphenols and polysaccharides, exerting prebiotics-like activities in the prevention and treatment of CMDs. The administrations of herbal medicine and functional food lead to increased the abundance of phylum Bacteroidetes, and genus Akkermansia, Bifidobacteria, Lactobacillus, Bacteroides and Prevotella, while reducing phylum Firmicutes and Firmicutes/Bacteroidetes ratio in gut...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29165477/barley-beta-glucans-varying-in-molecular-mass-and-oligomer-structure-affect-cecal-fermentation-and-microbial-composition-but-not-blood-lipid-profiles-in-hypercholesterolemic-rats
#15
Mette S Mikkelsen, Morten G Jensen, Tina S Nielsen
There is an unmet need for appealing and functional barley β-glucan (BG) food matrices that can provide sufficient and active BG doses to consumers. We investigated how molecular mass and oligomer structure important for BG food and health properties affected plasma lipids and gut parameters in hypercholesterolemic rats. Following 3 weeks on a high-cholestrol diet, rats were given a high-cholesterol diet supplemented with either cellulose (control) or purified barley BGs with low (100 or 150 kDa; glucagel or lowBG, respectively) or medium (530 kDa; mediumBG) molecular masses varying in cellotriosyl/cellotetraosyl oligomer ratio for 4 weeks...
November 22, 2017: Food & Function
https://www.readbyqxmd.com/read/29163454/low-molecular-weight-chitosan-supplementation-increases-the-population-of-prevotella-in-the-cecal-contents-of-weanling-pigs
#16
Ting Yu, Yu Wang, Shicheng Chen, Min Hu, Zhiling Wang, Guozhong Wu, Xianyong Ma, Zhuang Chen, Chuntian Zheng
Low-molecular-weight chitosan (LC) promoted growth in weaned piglets as an alternative to feed-grade antibiotics. To investigate the influence of LC supplementation on piglets' gut microbiome and compare the differences in community composition between LC and antibiotics with ZnO addition, we assessed the cecal microbial community by 16S rRNA gene sequencing with three treatments consisting of basal diet (CTR group), basal diet with low-molecular-weight chitosan (LC group), and basal diet with antibiotic and ZnO (AZ group)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163444/progressive-colonization-of-bacteria-and-degradation-of-rice-straw-in-the-rumen-by-illumina-sequencing
#17
Yanfen Cheng, Ying Wang, Yuanfei Li, Yipeng Zhang, Tianyi Liu, Yu Wang, Thomas J Sharpton, Weiyun Zhu
The aim of this study was to improve the utilization of rice straw as forage in ruminants by investigating the degradation pattern of rice straw in the dairy cow rumen. Ground up rice straw was incubated in situ in the rumens of three Holstein cows over a period of 72 h. The rumen fluid at 0 h and the rice straw at 0.5, 1, 2, 4, 6, 12, 24, 48, and 72 h were collected for analysis of the bacterial community and the degradation of the rice straw. The bacterial community and the carbohydrate-active enzymes in the rumen fluid were analyzed by metagenomics...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163431/camelina-seed-supplementation-at-two-dietary-fat-levels-change-ruminal-bacterial-community-composition-in-a-dual-flow-continuous-culture-system
#18
Xiaoxia Dai, Paul J Weimer, Kimberly A Dill-McFarland, Virginia L N Brandao, Garret Suen, Antonio P Faciola
This experiment aimed to determine the effects of camelina seed (CS) supplementation at different dietary fat levels on ruminal bacterial community composition and how it relates to changes in ruminal fermentation in a dual-flow continuous culture system. Diets were randomly assigned to 8 fermenters (1,200-1,250 mL) in a 2 × 2 factorial arrangement of treatments in a replicated 4 × 4 Latin square with four 10-day experimental periods that consisted of 7 days for diet adaptation and 3 days for sample collection...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163369/preconception-prebiotic-and-sitagliptin-treatment-in-obese-rats-affects-pregnancy-outcomes-and-offspring-microbiota-adiposity-and-glycemia
#19
Carol A Dennison, Amanda J Eslinger, Raylene A Reimer
Maternal obesity is associated with increased risk of pregnancy complications and greater risk of obesity in offspring, but studies designed to examine preconception weight loss are limited. The objective of this study was to determine if a combined dietary [oligofructose (OFS)] and pharmacological (sitagliptin) preconception intervention could mitigate poor pregnancy outcomes associated with maternal obesity and improve offspring metabolic health and gut microbiota composition. Diet-induced obese female Sprague-Dawley rats were randomized to one of four intervention groups for 8 weeks: (1) Obese-Control (consumed control diet during intervention); (2) Obese-OFS (10% OFS diet); (3) Obese-S (sitagliptin drug); (4) Obese-OFS + S (combination treatment)...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29147865/changes-of-gut-microbiota-structure-and-morphology-in-weaned-piglets-treated-with-fresh-fermented-soybean-meal
#20
Zhengjun Xie, Luansha Hu, Yuan Li, Shijie Geng, Saisai Cheng, Xiongfeng Fu, Shoumei Zhao, Xinyan Han
This study investigated the effects of dietary fresh fermented soybean meal (FSM) on the intestinal microbiota and metabolites, bacterial enzyme activity and intestinal morphology of weaning piglets. A total of 64 weaned piglets were randomly allocated into two treatments. A corn-soybean-based diet was used as the control and other treatment was fed the same basal diet containing 15% fresh FSM. The feeding trial lasted for 21 days. Bacterial community structure and diversity in the cecum and colon were assessed using pyrosequencing-based analysis...
November 16, 2017: World Journal of Microbiology & Biotechnology
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