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https://www.readbyqxmd.com/read/28222517/association-of-central-arterial-stiffness-and-pressure-pulsatility-with-mild-cognitive-impairment-and-dementia-the-atherosclerosis-risk-in-communities-study-neurocognitive-study-aric-ncs
#1
Michelle L Meyer, Priya Palta, Hirofumi Tanaka, Jennifer A Deal, Jacqueline Wright, David S Knopman, Michael Griswold, Thomas H Mosley, Gerardo Heiss
BACKGROUND: The association of central arterial stiffness and pressure pulsatility with mild cognitive impairment (MCI) and dementia is not well characterized in the population-based setting. OBJECTIVE: The aim of this study was to quantify the cross-sectional association of arterial stiffness and pressure pulsatility with MCI and dementia among 4,461 older white and black adults from the population-based Atherosclerosis Risk in Communities Study-Neurocognitive Study...
February 10, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28222424/1%C3%AE-25-oh-2d3-sensitive-cytosolic-ph-regulation-and-glycolytic-flux-in-human-endometrial-ishikawa-cells
#2
Ni Zeng, Yuetao Zhou, Shaqiu Zhang, Yogesh Singh, Bing Shi, Madhuri S Salker, Florian Lang
BACKGROUND/AIMS: Tumor cell proliferation is modified by 1,25-Dihydroxy-Vitamin D3 (1,25(OH)2D3), a steroid hormone predominantly known for its role in calcium and phosphorus metabolism. Key properties of tumor cells include enhanced glycolytic flux with excessive consumption of glucose and formation of lactate. As glycolysis is highly sensitive to cytosolic pH, maintenance of glycolysis requires export of H+ ions and lactate, which is in part accomplished by Na+/H+ exchangers, such as NHE1 and monocarboxylate transporters, such as MCT4...
February 8, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28222392/endor-with-band-selective-shaped-inversion-pulses
#3
Claudia E Tait, Stefan Stoll
Electron Nuclear DOuble Resonance (ENDOR) is based on the measurement of nuclear transition frequencies through detection of changes in the polarization of electron transitions. In Davies ENDOR, the initial polarization is generated by a selective microwave inversion pulse. The rectangular inversion pulses typically used are characterized by a relatively low selectivity, with full inversion achieved only for a limited number of spin packets with small resonance offsets. With the introduction of pulse shaping to EPR, the rectangular inversion pulses can be replaced with shaped pulses with increased selectivity...
February 12, 2017: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/28222197/generalized-min-max-bound-based-mri-pulse-sequence-design-framework-for-wide-range-t1-relaxometry-a-case-study-on-the-tissue-specific-imaging-sequence
#4
Yang Liu, John R Buck, Vasiliki N Ikonomidou
This paper proposes a new design strategy for optimizing MRI pulse sequences for T1 relaxometry. The design strategy optimizes the pulse sequence parameters to minimize the maximum variance of unbiased T1 estimates over a range of T1 values using the Cramér-Rao bound. In contrast to prior sequences optimized for a single nominal T1 value, the optimized sequence using our bound-based strategy achieves improved precision and accuracy for a broad range of T1 estimates within a clinically feasible scan time. The optimization combines the downhill simplex method with a simulated annealing process...
2017: PloS One
https://www.readbyqxmd.com/read/28222140/in-situ-single-cell-detection-via-microfluidic-magnetic-bead-assay
#5
Fan Liu, Pawan Kc, Ge Zhang, Jiang Zhe
We present a single cell detection device based on magnetic bead assay and micro Coulter counters. This device consists of two successive micro Coulter counters, coupled with a high gradient magnetic field generated by an external magnet. The device can identify single cells in terms of the transit time difference of the cell through the two micro Coulter counters. Target cells are conjugated with magnetic beads via specific antibody and antigen binding. A target cell traveling through the two Coulter counters interacts with the magnetic field, and have a longer transit time at the 1st counter than that at the 2nd counter...
2017: PloS One
https://www.readbyqxmd.com/read/28221976/listeria-monocytogenes-in-smoked-salmon-and-other-smoked-fish-at-retail-in-italy-frequency-of-contamination-and-strain-characterization-in-products-from-different-manufacturers
#6
Vicdalia Aniela Acciari, Marina Torresi, Luigi Iannetti, Silvia Scattolini, Francesco Pomilio, Lucia Decastelli, Silvia Colmegna, Riccardo Muliari, Teresa Bossù, Yolande Proroga, Cosimo Montagna, Cinzia Cardamone, Paola Cogoni, Vincenza Annunziata Prencipe, Giacomo Migliorati
Seven hundred seventy-eight samples of packaged smoked fish (774 smoked salmon and 4 smoked swordfish) on sale in Italy, from 50 different manufacturers located in 12 European Union countries, were purchased from the Italian market between May and December 2011. The surface temperatures of the samples on sale ranged from 0 to 13°C (3.4 ± 1.5°C, mean ± SD). Six hundred eighty (87.4%) of 778 samples were stored at ≤4°C. One hundred fifty-seven samples (20.2%, 95% confidence interval 17.5 to 23.1%) were contaminated by Listeria monocytogenes , with 26 samples (3...
February 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221971/characterizing-salmonella-contamination-in-two-rendering-processing-plants
#7
Chao Gong, Xiuping Jiang
A microbiological investigation on Salmonella contamination was conducted in two U.S. rendering plants to investigate the potential cross-contamination of Salmonella in the rendering processing environment. Sampling locations were predetermined at the areas where Salmonella contamination may potentially occur, including raw materials receiving, crax (rendered materials before grinding process) grinding, and finished meal loading-out areas. Salmonella was either enumerated directly on xylose lysine Tergitol 4 agar plates or enriched in Rappaport-Vassiliadis and tetrathionate broths...
February 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221969/development-and-validation-of-pathogen-environmental-monitoring-programs-for-small-cheese-processing-facilities
#8
Sarah M Beno, Matthew J Stasiewicz, Alexis D Andrus, Robert D Ralyea, David J Kent, Nicole H Martin, Martin Wiedmann, Kathryn J Boor
Pathogen environmental monitoring programs (EMPs) are essential for food processing facilities of all sizes that produce ready-to-eat food products exposed to the processing environment. We developed, implemented, and evaluated EMPs targeting Listeria spp. and Salmonella in nine small cheese processing facilities, including seven farmstead facilities. Individual EMPs with monthly sample collection protocols were designed specifically for each facility. Salmonella was detected in only one facility, with likely introduction from the adjacent farm indicated by pulsed-field gel electrophoresis data...
December 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221950/multistate-outbreak-of-escherichia-coli-o157-h7-infections-associated-with-consumption-of-fresh-spinach-united-states-2006
#9
Umid M Sharapov, Arthur M Wendel, Jeffrey P Davis, William E Keene, Jeffrey Farrar, Samir Sodha, Eija Hyytia-Trees, Molly Leeper, Peter Gerner-Smidt, Patricia M Griffin, Chris Braden
During September to October, 2006, state and local health departments and the Centers for Disease Control and Prevention investigated a large, multistate outbreak of Escherichia coli O157:H7 infections. Case patients were interviewed regarding specific foods consumed and other possible exposures. E. coli O157:H7 strains isolated from human and food specimens were subtyped using pulsed-field gel electrophoresis and multiple-locus variable-number tandem repeat analyses (MLVA). Two hundred twenty-five cases (191 confirmed and 34 probable) were identified in 27 states; 116 (56%) case patients were hospitalized, 39 (19%) developed hemolytic uremic syndrome, and 5 (2%) died...
December 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221920/prevalence-and-molecular-characterization-of-methicillin-resistant-staphylococcus-aureus-in-foods-of-animal-origin-turkey
#10
Belgin Siiriken, Tuba Yildirim, Akif Koray Güney, Irfan Erol, Belma Durupinar
In the present study, 175 coagulase-positive Staphylococcus (CPS) isolates recovered from samples of beef (n = 110), raw milk n = 56), and fish (n = 9) were analyzed for methicillin resistance using MIC and PCR assays. Methicillin-resistant (MR) Staphylococcus aureus (SA) isolates were then characterized using pulsed-field gel electrophoresis (PFGE). According to findings, 62 (35.4%) of the isolates (44 from beef, 9 from milk, and 9 from fish) were identified as S. aureus based on the presence of the nuc gene...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221916/characterization-and-antimicrobial-resistance-of-salmonella-typhimurium-isolates-from-clinically-diseased-pigs-in-korea
#11
Sang-Ik Oh, Jong Wan Kim, Myeongju Chae, Ji-A Jung, Byungjae So, Bumseok Kim, Ha-Young Kim
This study investigated the prevalence of Salmonella enterica serovar and antimicrobial resistance in Salmonella Typhimurium isolates from clinically diseased pigs collected from 2008 to 2014 in Korea. Isolates were also characterized according to the presence of antimicrobial resistance genes and pulsed-field gel electrophoresis patterns. Among 94 Salmonella isolates, 81 (86.2%) were identified as being of the Salmonella Typhimurium serotype, followed by Salmonella Derby (6 of 94, 6.4%), Salmonella 4,[5],12:i:- (4 of 94, 4...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221912/isolation-identification-and-evaluation-of-new-lactic-acid-bacteria-strains-with-both-cellular-antioxidant-and-bile-salt-hydrolase-activities-in-vitro
#12
Shuang Xu, Taigang Liu, Chiraz Akorede Ibinke Radji, Jing Yang, Lanming Chen
In this study, we analyzed Chinese traditional fermented food to isolate and identify new lactic acid bacteria (LAB) strains with novel functional properties and to evaluate their cellular antioxidant and bile salt hydrolase (BSH) activities in vitro. A sequential screening strategy was developed to efficiently isolate and obtain 261 LAB strains tolerant of bile salt, acid, and H2O2 from nine Chinese traditional fermented foods. Among these strains, 70 were identified as having 2,2-diphenyl-1-picrylhydrazyl radical scavenging and/or BSH activity...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221911/molecular-subtyping-and-source-attribution-of-campylobacter-isolated-from-food-animals
#13
Gregory H Tyson, Heather P Tate, Jason Abbott, Thu-Thuy Tran, Claudine Kabera, Emily Crarey, Shenia Young, Patrick F McDERMOTT, Grisselle Sprague, Mark Campbell, Oyewole Adeyemo, Johnette Browne-Silva, Michael Myers, Sutawee Thitaram, Shaohua Zhao
Campylobacter spp. commonly cause gastrointestinal illness in humans. Poultry meats have long been considered the predominant source of these infections, but few in-depth Campylobacter source attribution studies have been completed. We analyzed more than 1,300 Campylobacter isolates recovered from a number of animal and food sources, including dairy and beef cattle, pigs, poultry, and retail poultry meat, and compared them with Campylobacter isolates recovered from human clinical samples. Each isolate was subtyped using pulsed-field gel electrophoresis (PFGE) with SmaI and queried against the Centers for Disease Control and Prevention PulseNet database to identify human isolates with indistinguishable patterns...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221901/prevalence-and-persistence-of-listeria-monocytogenes-in-ready-to-eat-tilapia-sashimi-processing-plants
#14
Bang-Yuan Chen, Chung-Yi Wang, Chia-Lan Wang, Yang-Chi Fan, I-Ting Weng, Chung-Hsi Chou
A 2-year study was performed at two ready-to-eat tilapia sashimi processing plants (A and B) to identify possible routes of contamination with Listeria monocytogenes during processing. Samples were collected from the aquaculture environments, transportation tanks, processing plants, and final products. Seventy-nine L. monocytogenes isolates were found in the processing environments and final products; 3.96% (50 of 1,264 samples) and 3.86% (29 of 752 samples) of the samples from plants A and B, respectively, were positive for L...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221883/enrichment-amplification-and-sequence-based-typing-of-salmonella-enterica-and-other-foodborne-pathogens
#15
Tom Edlind, Jeffrey D Brewster, George C Paoli
Detection of Salmonella enterica in foods typically involves microbiological enrichment, molecular-based assay, and subsequent isolation and identification of a pure culture. This is ideally followed by strain typing, which provides information critical to the investigation of outbreaks and the attribution of their sources. Pulsed-field gel electrophoresis is the "gold standard" for S. enterica strain typing, but its limitations have encouraged the search for alternative methods, including whole genome sequencing...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221879/antimicrobial-susceptibility-and-molecular-typing-of-salmonella-senftenberg-isolated-from-humans-and-other-sources-in-shanghai-china-2005-to-2011
#16
Zeqiang Zhan, Dai Kuang, Ming Liao, Hongxia Zhang, Jingzhang Lu, Xueming Hu, Yulong Ye, Jianghong Meng, Xuebin Xu, Jianmin Zhang
Salmonella Senftenberg is an important nontyphoidal Salmonella serovar that causes gastrointestinal disease worldwide. In total, 130 Salmonella Senftenberg strains obtained from humans, food, and the environment in Shanghai, People's Republic of China, were characterized for antimicrobial susceptibility and subjected to molecular typing. Our findings indicated that most (96 of 130, 73.8%) of the strains were susceptible to all 13 antimicrobial compounds tested, whereas only two strains (1.5%) were resistant to two antimicrobial compounds...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221858/genotypic-and-phenotypic-characterization-of-methicillin-resistant-staphylococcus-aureus-isolated-from-bovine-mastitic-milk-in-korea
#17
Jae Won Song, Soo Jin Yang, Sook Shin, Keun Seok Seo, Yong Ho Park, Kun Taek Park
Staphylococcus aureus is a major etiological pathogen for bovine mastitis, foodborne illness, and various clinical infections. Methicillin-resistant S. aureus (MRSA) has been isolated from bovine mastitic milk, and the presence of MRSA in milk is a major public health concern. We investigated the frequency of MRSA isolation from mastitic raw milk in Korea and characterized the patterns of antimicrobial resistance, virulence, and genotypes of the MRSA isolates. A total of 1,222 raw milk samples were collected from 47 dairy farms in Gyeonggi province from 2011 to 2012...
October 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221372/neglecting-legumes-has-compromised-human-health-and-sustainable-food-production
#18
Christine H Foyer, Hon-Ming Lam, Henry T Nguyen, Kadambot H M Siddique, Rajeev K Varshney, Timothy D Colmer, Wallace Cowling, Helen Bramley, Trevor A Mori, Jonathan M Hodgson, James W Cooper, Anthony J Miller, Karl Kunert, Juan Vorster, Christopher Cullis, Jocelyn A Ozga, Mark L Wahlqvist, Yan Liang, Huixia Shou, Kai Shi, Jingquan Yu, Nandor Fodor, Brent N Kaiser, Fuk-Ling Wong, Babu Valliyodan, Michael J Considine
The United Nations declared 2016 as the International Year of Pulses (grain legumes) under the banner 'nutritious seeds for a sustainable future'. A second green revolution is required to ensure food and nutritional security in the face of global climate change. Grain legumes provide an unparalleled solution to this problem because of their inherent capacity for symbiotic atmospheric nitrogen fixation, which provides economically sustainable advantages for farming. In addition, a legume-rich diet has health benefits for humans and livestock alike...
August 2, 2016: Nature Plants
https://www.readbyqxmd.com/read/28221022/quantitative-13c-traces-of-glucose-fate-in-hepatitis-b-virus-infected-hepatocytes
#19
Qianfen Wan, Yulan Wang, Huiru Tang
Quantitative characterization of 13C-labeled metabolites is an important part of the stable isotope tracing method widely used in metabolic flux analysis. Due to long relaxation time and low sensitivity of 13C nuclei, direct measurement of 13C labeled metabolites using one dimensional 13C NMR often fails to meet the demand of metabolomics studies especially with large number of samples and metabolites having low abundance. Although HSQC-based 2D NMR methods have improved sensitivity with inversion detection, they are time-consuming thus unsuitable for high-throughput absolute quantification of 13C-labeled metabolites...
February 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28220893/general-planar-transverse-domain-walls-realized-by-optimized-transverse-magnetic-field-pulses-in-magnetic-biaxial-nanowires
#20
Mei Li, Jianbo Wang, Jie Lu
The statics and field-driven dynamics of transverse domain walls (TDWs) in magnetic nanowires (NWs) have attracted continuous interests because of their theoretical significance and application potential in future magnetic logic and memory devices. Recent results demonstrate that uniform transverse magnetic fields (TMFs) can greatly enhance the wall velocity, meantime leave a twisting in the TDW azimuthal distribution. For application in high-density NW devices, it is preferable to erase the twisting so as to minimize magnetization frustrations...
February 21, 2017: Scientific Reports
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