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https://www.readbyqxmd.com/read/28812572/population-characteristics-of-a-large-whale-shark-aggregation-inferred-from-seawater-environmental-dna
#1
Eva Egelyng Sigsgaard, Ida Broman Nielsen, Steffen Sanvig Bach, Eline D Lorenzen, David Philip Robinson, Steen Wilhelm Knudsen, Mikkel Winther Pedersen, Mohammed Al Jaidah, Ludovic Orlando, Eske Willerslev, Peter Rask Møller, Philip Francis Thomsen
Population genetics is essential for understanding and managing marine ecosystems, but sampling remains challenging. We demonstrate that high-throughput sequencing of seawater environmental DNA can provide useful estimates of genetic diversity in a whale shark (Rhincodon typus) aggregation. We recover similar mitochondrial haplotype frequencies in seawater compared to tissue samples, reliably placing the studied aggregation in a global genetic context and expanding the applications of environmental DNA to encompass population genetics of aquatic organisms...
November 21, 2016: Nature ecology & evolution
https://www.readbyqxmd.com/read/28811301/topically-applied-carvedilol-attenuates-solar-ultraviolet-radiation-induced-skin-carcinogenesis
#2
Kevin M Huang, Sherry Liang, Steven Yeung, Etuajie Oiyemhonlan, Kristan H Cleveland, Cyrus Parsa, Robert Orlando, Frank L Meyskens, Bradley Andresen, Ying Huang
In previous studies, the β-blocker carvedilol inhibited EGF-induced epidermal cell transformation and chemical carcinogen-induced mouse skin hyperplasia. As exposure to ultraviolet (UV) radiation leads to skin cancer, the present study examined whether carvedilol can prevent UV-induced carcinogenesis. Carvedilol absorbs UV like a sunscreen; thus, to separate pharmacological from sunscreen effects, 4-hydroxycarbazole (4-OHC), which absorbs UV to the same degree as carvedilol, served as control. JB6 P+ cells, an established epidermal model for studying tumor promotion, were used for evaluating the effect of carvedilol on UV-induced neoplastic transformation...
August 15, 2017: Cancer Prevention Research
https://www.readbyqxmd.com/read/28808457/physicochemical-properties-of-mta-and-portland-cement-after-addition-of-aloe-vera
#3
Alvaro Henrique Borges, Orlando Aguirre Guedes, Luiz Evaristo Ricci Volpato, Gilberto Siebert Filho, Alexandre Meireles Borba, Omar Zina, Evandro Piva, Carlos Estrela
INTRODUCTION: The aim of this in vitro study was to determine the liquid-powder ratio, setting time, solubility, dimensional change, pH, and radiopacity of white structural and non-structural Portland cement, ProRoot MTA and MTA Bio, associated with a 2% glycolic solution containing Aloe Vera, as vehicle. METHODS AND MATERIALS: Five samples of each material were used for each test, according to the American National Standards Institute/American Dental Association (ANSI/ADA) specification No...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28806562/zika-virus-induced-neurological-critical-illness-in-latin-america-severe-guillain-barre-syndrome-and-encephalitis
#4
Sebastián U Ugarte, Angel Ricardo V Arenas, Bruno C Alvarez, Angela Cubides, Angélica F Luna, Max Arroyo-Parejo, Cayri E Acuña, Agamenón V Quintero, Orlando Ch Villareal, Oscar S Pinillos, Elías Vieda, Manuel Bello, Susana Peña, Carmelo Dueñas-Castell, Gloria M V Rodriguez, Jorge L M Ranero, Rosa L M López, Sandra G Olaya, José C Vergara, Ana Tandazo, Juan P S Ospina, Igor M Leyton Soto, R A Fowler, John C Marshall
Zika virus (ZIKAV) is classically described as causing minor symptoms in adult patients, however neurologic complications have been recognized. The recent outbreak in Central and South America has resulted in serious illness in some adult patients. We report adult patients in Latin America diagnosed with ZIKAV infection admitted to Intensive Care Units (ICUs). METHODS: Multicenter, prospective case series of adult patients with laboratory diagnosis of ZIKAV in 16 ICUs in 8 countries. RESULTS: Between December 1st 2015 and April 2nd 2016, 16 ICUs in 8 countries enrolled 49 critically ill patients with diagnosis of ZIKAV infection...
August 9, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/28803236/enhancement-of-bone-healing-by-local-administration-of-carbon-nanotubes-functionalized-with-sodium-hyaluronate-in-rat-tibiae
#5
Vanessa B Andrade, Marcos A Sá, Renato M Mendes, Paulo A Martins-Júnior, Gerluza A B Silva, Bruna R Sousa, Marcelo V Caliari, Erick S Ávila, Luiz Orlando Ladeira, Rodrigo R Resende, Anderson J Ferreira
It has been reported that carbon nanotubes (CNTs) serve as nucleation sites for the deposition of bone matrix and cell proliferation. Here, we evaluated the effects of multi-walled CNTs (MWCNTs) on bone repair of rat tibiae. Furthermore, because sodium hyaluronate (HY) accelerates bone restoration, we associated CNTs with HY (HY-MWCNTs) in an attempt to boost bone repair. The bone defect was created by a 1.6-mm-diameter drill. After 7 and 14 days, tibiae were processed for histological and morphometric analyses...
August 12, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/28802769/electrophysiological-correlates-of-voice-memory-for-young-and-old-speakers-in-young-and-old-listeners
#6
Romi Zäske, Katharina Limbach, Dana Schneider, Verena G Skuk, Christian Dobel, Orlando Guntinas-Lichius, Stefan Robert Schweinberger
Faces of one's own-age group are easier to recognize than other-age faces. Using behavioural measures and EEG, we studied whether an own-age bias (OAB) also exists in voice memory. Young (19 - 26 years) and old (60 - 75 years) participants studied young (18 - 25 years) and old (60 - 77 years) unfamiliar voices from short sentences. Subsequently, they classified studied and novel voices as "old" (i.e. studied) or "new", from the same sentences. Recognition performance was higher in young compared to old participants, and for old compared to young voices, with no OAB...
August 9, 2017: Neuropsychologia
https://www.readbyqxmd.com/read/28801407/the-boundaries-of-mild-chronic-obstructive-pulmonary-disease-copd-design-of-the-searching-clinical-copd-onset-soon-study
#7
Gonzalo Labarca, Andrea Bustamante, Gonzalo Valdivia, Rodrigo Díaz, Álvaro Huete, Paul Mac Nab, Laura Mendoza, Jaime Leppe, Carmen Lisboa, Fernando Saldías, Orlando Díaz
INTRODUCTION: Clinical onset of chronic obstructive pulmonary disease (COPD) is the point at which the disease is first identifiable by physicians. It is a poorly defined stage which seems to include both mild spirometric and non-spirometric disease, and could be described as early grade COPD, for practical purposes. While dyspnoea; chronic bronchitis and CT imaging evidence of emphysema and airway disease may be present very early, the lone significance of dyspnoea, the most relevant symptom in COPD in identifying these individuals, has been scarcely assessed...
August 11, 2017: BMJ Open
https://www.readbyqxmd.com/read/28800365/the-impact-of-the-orlando-mass-shooting-on-fear-of-victimization-and-gun-purchasing-intentions-not-what-one-might-expect
#8
Wolfgang Stroebe, N Pontus Leander, Arie W Kruglanski
Mass public shootings are typically followed by a spike in gun sales as well as calls for stricter gun control laws. What remains unclear is whether the spike in gun sales is motivated by increased threat perceptions or by concerns about gun control, or whether the sales are mainly driven by non-owners purchasing guns or gun owners adding to their collection. Two surveys of gun owners and non-owners, conducted immediately before and after the Orlando shooting, allowed us to assess its impact on threat perceptions and on gun-purchasing intentions...
2017: PloS One
https://www.readbyqxmd.com/read/28800160/exercise-attenuates-alveolar-bone-loss-and-anxiety-like-behaviour-in-rats-with-periodontitis
#9
Eric F Andrade, Débora R Orlando, Júlia A S Gomes, Renata de C Foureaux, Rafael C Costa, Mary S Varaschin, Gustavo P Rogatto, Rodrigo F de Moura, Luciano J Pereira
AIM: To evaluate the effects of physical training on inflammatory and behavioral parameters of Wistar rats with periodontal disease (PD). MATERIALS AND METHODS: Twenty four animals were distributed in a 2x2 factorial design (with and without exercise, with and without PD). Trained animals swimmed one hour daily during eight weeks. PD was induced by ligature 14 days before the end of experiment and in the last week all animals were submitted to the Marble Burying Test...
August 11, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28797663/improvement-of-olfactory-function-after-sinus-surgery-correlates-with-white-matter-properties-measured-by-diffusion-tensor-imaging
#10
Daniel Güllmar, Tabea Seeliger, Hilmar Gudziol, Ulf K M Teichgräber, Jürgen R Reichenbach, Orlando Guntinas-Lichius, Thomas Bitter
Impaired olfaction is associated with a volume decrease in the olfactory bulb as well as in the gray matter of cortical olfactory areas. On the other hand, restitution of an impaired olfaction results in a regain of volume in these regions. Studies investigating similar changes in the cerebral white matter are virtually not existent. The aim of this prospective study therefore was to investigate cerebral white matter using magnetic resonance diffusion tensor imaging (DTI). 31 patients (54±13years) with olfactory impairment (chronic rhinosinusitis) and planned functional endoscopic sinus surgery (FESS) were included...
August 7, 2017: Neuroscience
https://www.readbyqxmd.com/read/28795611/the-relationship-of-repeated-technical-assistance-support-visits-to-the-delivery-of-positive-health-dignity-and-prevention-phdp-messages-by-healthcare-providers-in-mozambique-a-longitudinal-multilevel-analysis
#11
Sarah A Gutin, K Rivet Amico, Elsa Hunguana, António Orlando Munguambe, Carol Dawson Rose
BACKGROUND: Positive health, dignity, and prevention (PHDP) is Mozambique's strategy to engage clinicians in the delivery of prevention messages to their HIV-positive clients. This national implementation strategy uses provider trainings on offering key messages and focuses on intervening on 9 evidence-based risk reduction areas. We investigated the impact of longitudinal technical assistance (TA) as an addition to this basic training. METHODS: We followed 153 healthcare providers in 5 Mozambican provinces over 6 months to evaluate the impact of on-site, observation-based TA on PHDP implementation...
January 1, 2017: Journal of the International Association of Providers of AIDS Care
https://www.readbyqxmd.com/read/28782559/trap-seq-an-rna-sequencing-based-pipeline-for-the-identification-of-genetrap-insertions-in-mammalian-cells
#12
Cristina Mayor-Ruiz, Orlando Dominguez, Oscar Fernandez-Capetillo
The development of haploid mammalian cell lines, coupled to next generation sequencing, has recently facilitated forward genetic screenings in mammals. For mutagenesis, retrovirus- or transposon-based genetraps are frequently used. Current methods to map genetrap insertions are based on inverse- or splinkerette-PCR, which despite their efficacy are prone to artifacts and do not provide information about expression of the targeted gene. Here, we describe a new RNA-sequencing based method (Trap(Seq)) to map genetrap insertions...
August 4, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28780446/biochemical-responses-to-cadmium-exposure-in-oncorhynchus-mykiss-erythrocytes
#13
Patrick Orlando, Sonia Silvestri, Enea Ferlizza, Giulia Andreani, Emilio Carpenè, Giancarlo Falcioni, Luca Tiano, Gloria Isani
Cd is known for its carcinogenic effects, however its mechanism of toxicity and in particular its ability to promote oxidative stress is debated. In fact, although it is considered a redox-inactive metal, at high concentration Cd was shown to promote indirectly oxidative stress. In this study we investigated metal accumulation in ex vivo exposed trout (Oncorhynchus mykiss) erythrocytes and Cd dose-dependent effect in terms of RBC viability, cytosolic and mitochondrial ROS levels as well as its effects on mitochondrial membrane depolarization, hemoglobin stability and precipitation...
August 3, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28779802/editorial-commentary-is-anterolateral-ligament-reconstruction-of-the-knee-needed-the-debate-rages-on
#14
EDITORIAL
Bruce A Levy, Orlando D Sabbag
A recent systematic review on the techniques, biomechanics, and clinical outcomes of anterolateral ligament reconstruction of the knee summarizes our current understanding of this hotly debated topic. In 2017, it is not clear if an anterolateral ligament reconstruction is needed in the setting of primary anterior cruciate ligament reconstruction.
August 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28774762/epidemiology-of-mild-traumatic-brain-injury-with-intracranial-hemorrhage-focusing-predictive-models-for-neurosurgical-intervention
#15
Alessandro Orlando, A Stewart Levy, Matthew M Carrick, Allen Tanner, Charles W Mains, David Bar-Or
OBJECTIVE: To adumbrate differences in neurosurgical intervention (NI) rates between intracranial hemorrhage (ICH) types in mild traumatic brain injuries (mTBIs), and help identify which ICH types are most likely to benefit from the creation of predictive models for NI. METHODS: This was a multi-center retrospective study of adult patients over three years at four Trauma Centers in the USA. Patients were included if they presented with a mTBI (GCS 13-15) and a head CT positive for ICH...
July 31, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28773001/effect-of-different-carbon-sources-on-bacterial-nanocellulose-production-and-structure-using-the-low-ph-resistant-strain-komagataeibacter-medellinensis
#16
Carlos Molina-Ramírez, Margarita Castro, Marlon Osorio, Mabel Torres-Taborda, Beatriz Gómez, Robin Zuluaga, Catalina Gómez, Piedad Gañán, Orlando J Rojas, Cristina Castro
Bacterial cellulose (BC) is a polymer obtained by fermentation with microorganism of different genera. Recently, new producer species have been discovered, which require identification of the most important variables affecting cellulose production. In this work, the influence of different carbon sources in BC production by a novel low pH-resistant strain Komagataeibacter medellinensis was established. The Hestrin-Schramm culture medium was used as a reference and was compared to other media comprising glucose, fructose, and sucrose, used as carbon sources at three concentrations (1, 2, and 3% w/v)...
June 11, 2017: Materials
https://www.readbyqxmd.com/read/28772191/synthetic-genetic-circuits-in-crop-plants
#17
REVIEW
Orlando de Lange, Eric Klavins, Jennifer Nemhauser
The love affair between crop breeding and genetics began over a century ago and has continued unabated, from mass selection programs to targeted genome modifications. Synthetic genetic circuits, a recent development, are combinations of regulatory and coding DNA introduced into a crop plant to achieve a desired function. Genetic circuits could accelerate crop improvement, allowing complex traits to be rationally designed and requisite DNA parts delivered directly into a genome of interest. However, there is not yet a standardized pipeline from exploratory laboratory testing to crop trials, and bringing transgenic products to market remains a considerable barrier...
July 31, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28771873/nitrate-induction-of-root-hair-density-is-mediated-by-tga1-tga4-and-cpc-transcription-factors-in-arabidopsis-thaliana
#18
Javier Canales, Orlando Contreras-López, José M Álvarez, Rodrigo A Gutiérrez
Root hairs are specialized cells important for nutrient uptake. It is well established that nutrients such as phosphate have great influence on root hair development in many plant species. Here, we investigated the role of nitrate on root hair development at a physiological and molecular level. We showed nitrate increase root hair density in Arabidopsis thaliana. We found two different root hair defective mutants have significantly less nitrate than wild type plants, suggesting root hairs have an important role in nitrate acquisition capacity in A...
August 3, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28768355/-placement-of-a-tracheobronchial-bifurcation-stent-for-treatment-of-tumor-related-tracheobronchial-stenoses-and-fistulas
#19
Julia Ritter, Orlando Guntinas-Lichius, Thomas Bitter
No abstract text is available yet for this article.
July 2017: Laryngo- Rhino- Otologie
https://www.readbyqxmd.com/read/28763512/a-glycan-based-approach-to-therapeutic-angiogenesis
#20
Jie Shi Chua, Vy M Tran, Mausam Kalita, Maritza V Quintero, Orlando Antelope, Geethu Muruganandam, Yukio Saijoh, Balagurunathan Kuberan
Angiogenesis, the sprouting of new blood vessels from existing vasculature, involves multiple complex biological processes, and it is an essential step for hemostasis, tissue healing and regeneration. Angiogenesis stimulants can ameliorate human disease conditions including limb ischemia, chronic wounds, heart disease, and stroke. The current strategies to improve the bioavailability of pro-angiogenic growth factors, including VEGF and FGF2, have remained largely unsuccessful. This study demonstrates that small molecules, termed click-xylosides, can promote angiogenesis in the in vitro matrigel tube formation assay and the ex ovo chick chorioallantoic membrane assay, depending on their aglycone moieties...
2017: PloS One
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