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https://www.readbyqxmd.com/read/28535611/sea-buckthorn-hippophae-rhamnoides-l-leaf-extracts-protect-neuronal-pc-12-cells-from-oxidative-stress
#1
Chi Heung Cho, Ho Lim Jang, Migi Lee, Hee Kang, Ho Jjn Heo, Dae-Ok Kim
The present study was carried out to investigate the antioxidative and neuroprotective effects of sea buckthorn (Hippophae rhamnoides L.) leaves (SBL) harvested at different times. Reversed-phase high-performance liquid chromatography analysis revealed five major phenolic compounds: ellagic acid, gallic acid, isorhamnetin, kaempferol, and quercetin. SBL harvested in August had the highest total phenolic and flavonoid contents and antioxidant capacity. Treatment of neuronal PC-12 cells with the ethyl acetate fraction of SBL harvested in August in increased their viability and membrane integrity and reduced intracellular oxidative stress in a dose-dependent manner...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28535606/isolation-purification-and-characterization-of-five-active-diketopiperazine-derivatives-from-endophytic-streptomyces-suk-25-with-antimicrobial-and-cytotoxic-activities
#2
Muhanna M Alshaibani, Juriyati Jalil, Nik M Sidik, Siti Junaidah Ahmad, Nurkhalida Kamal, Ruangelie Edrada-Ebel, Noraziah M Zin
On our search for new sources of bioactive secondary metabolites from Streptomyces sp., the ethyl acetate extracts from the endophytic Streptomyces SUK 25 afforded five active diketopiperazines compounds. The aim of this study was to characterize the bioactive compounds isolated from endophytic Streptomyces SUK 25 and evaluate its bioactivity against multiple drug resistance (MDR) bacteria such as Enterococcus raffinosus, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa and Enterobacter spp...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28535597/variation-and-accumulation-patterns-of-poly-and-perfluoroalkyl-substances-pfas-in-european-perch-perca-fluviatilis-across-a-gradient-of-pristine-swedish-lakes
#3
Staffan Åkerblom, Nesrin Negm, Pianpian Wu, Kevin Bishop, Lutz Ahrens
This study assessed variations in the concentrations of poly- and perfluoroalkyl substances (PFAS) in European perch (Perca fluviatilis) in Swedish lakes and the extent to which fish size, age and indicators of fish trophic ecology (δ(15)N and δ(13)C) correlate with the sum of individual PFAS concentrations (ΣPFAS). Fish muscle tissue samples (n=80) were taken from six lakes across Sweden and analysed using solid-liquid extraction followed by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS)...
May 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28535482/multiple-metal-exposures-and-their-correlation-with-monoamine-neurotransmitter-metabolism-in-chinese-electroplating-workers
#4
Lin-Lin Wu, Wei Gong, Si-Peng Shen, Zhong-He Wang, Jia-Xi Yao, Jun Wang, Jing Yu, Rong Gao, Gang Wu
Excessive metal exposure has been recognized as one of the detrimental factors for brain damage. However, the potential adverse effects induced by heavy metals on monoamine neurotransmitter pathways remains poorly understood. Our study aimed to investigate the possible association between metal exposure and neurotransmitter metabolism. By a cross-sectional investigation, 224 electroplating workers and 213 non-electroplating exposure workers were recruited in the exposure and control groups. Metal exposure levels were analyzed using inductively-coupled plasma mass spectrometry and monoamine neurotransmitter pathway metabolites were measured by ultra-performance liquid chromatography tandem mass spectrometry in human urine samples...
April 26, 2017: Chemosphere
https://www.readbyqxmd.com/read/28535464/occurrence-of-marine-algal-toxins-in-oyster-and-phytoplankton-samples-in-daya-bay-south-china-sea
#5
Tao Jiang, Lei Liu, Yang Li, Jing Zhang, Zhijun Tan, Haiyan Wu, Tianjiu Jiang, Songhui Lu
The occurrence and seasonal variations of marine algal toxins in phytoplankton and oyster samples in Daya Bay (DYB), South China Sea were investigated. Two Dinophysis species, namely, D. caudata and D. acuminata complex, were identified as Okadaic acid (OA)/pectenotoxin (PTX) related species. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis demonstrated that 2.04-14.47 pg PTX2 per cell was the predominant toxin in single-cell isolates of D. caudata. D. acuminata was not subjected to toxin analysis...
May 12, 2017: Chemosphere
https://www.readbyqxmd.com/read/28535452/highly-sensitive-uhplc-ms-ms-method-for-the-simultaneous-estimation-of-propafenone-and-its-metabolites-5-hydroxypropafenone-and-n-depropylpropafenone-on-human-dried-blood-spots-technique-and-application-to-a-pharmacokinetic-study
#6
Adinarayana Andy, Raja Reddy Kallem, Ramesh Mullangi, Divya Andy, J V L N Seshagiri Rao
A highly sensitive, rapid and selective UHPLC-MS/MS method has been developed and validated for quantification of the propafenone (PF), 5-hydroxypropafenone (5-OHPF) and N-depropylpropafenone (N-DPF) on human dried blood spot (DBS). The assay procedure involves a solid-liquid extraction of PF, 5-OHPF and N-DPF and amlodipine (internal standard, I.S.) from dried human DBS cards using water and acetonitrile. The chromatographic resolution was achieved on a BEH C18 column using a gradient mobile phase consisting of 0...
May 11, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28535438/determination-of-edoxaban-equivalent-concentrations-in-human-plasma-by-an-automated-anti-factor-xa-chromogenic-assay
#7
Ling He, Jarema Kochan, Min Lin, Alexander Vandell, Karen Brown, Francois Depasse
INTRODUCTION: This phase I, open-label, multiple-dose, two-treatment study assessed the relationship between edoxaban equivalent concentration derived from an anti-FXa assay with the summed concentration of edoxaban and its active metabolite, M-4, as assessed by liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). This study also assessed the relationship between edoxaban plasma concentrations assessed by LC/MS/MS in sodium citrate and lithium heparin tubes. MATERIALS AND METHODS: Healthy volunteers were randomized to receive once-daily edoxaban 60mg or 90mg for 5days (15 participants per treatment group)...
May 7, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28535423/chromatographic-separation-and-detection-methods-of-aloe-arborescens-miller-constituents-a-systematic-review
#8
REVIEW
Jilan A Nazeam, Haidy A Gad, Hala M El-Hefnawy, Abdel-Naser B Singab
Aloe arborescens Miller (Family Asphodelaceae) is a member of genus Aloe, which is used in traditional medicine to cure various diseases. The extracts of the plant have been reported to possess anticancer, immunomodulator, antidiabetic, anti-inflammatory and antioxidant activities. The phytochemical investigations have revealed diverse chemical constituents, including phenolics [anthraquinones, anthrones, pyrones, chromones and coumarins], polysaccharides [arborans [(1-4) linked glucomannans, polysaccharide (A, B and C): (A: a linear (1-6)-O-α-glucan, B: a branching (1-2)-O-l-arabinose with (1-2)-O-d-galactose linkages and C: (1-4)-O-β-mannan with 18% acetyl group)]], glycoproteins and carboxypeptidase enzyme...
April 29, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28535335/non-zero-ideal-gas-contribution-to-the-surface-tension-of-water
#9
Marcello Sega, Balázs Fábián, Pal Jedlovszky
Surface tension, the tendency of fluid interfaces to behave elastically and minimize their surface, is routinely calculated as the difference between the lateral and normal components of the pressure or, invoking isotropy in momentum space, of the virial tensor. Here we show that the anisotropy of the kinetic energy tensor close to a liquid/vapour interface can be responsible for a large part of its surface tension (about 15 % for water, independently on temperature).
May 23, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28535292/adaptive-radiation-of-p-fluorescens-sbw25-in-experimental-microcosms-provides-an-understanding-of-the-evolutionary-ecology-and-molecular-biology-of-a-l-interface-biofilm-formation
#10
Anna Koza, Anna Kusmierska, Kimberley McLaughlin, Olena Moshynets, Andrew J Spiers
Combined experimental evolutionary and molecular biology approaches have been used to investigate the adaptive radiation of Pseudomonas fluorescens SBW25 in static microcosms leading to the colonisation of the air-liquid interface by biofilm-forming mutants such as the Wrinkly Spreader. In these microcosms, the ecosystem engineering of the early wild-type colonists establish the niche space for subsequent WS evolution and colonisation. Random WS mutations occurring in the developing population that de-regulate diguanylate cyclases and c-di-GMP homeostasis result in cellulose-based biofilms at the air-liquid interface...
May 23, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28535204/colocalization-of-receptors-for-shiga-toxins-with-lipid-rafts-in-primary-human-renal-glomerular-endothelial-cells-and-influence-of-d-pdmp-on-synthesis-and-distribution-of-glycosphingolipid-receptors
#11
Nadine Legros, Gottfried Pohlentz, Jana Runde, Stefanie Dusny, Hans-Ulrich Humpf, Helge Karch, Johannes Müthing
Damage of human renal glomerular endothelial cells (HRGECs) of the kidney represents the linchpin in the pathogenesis of the hemolytic uremic syndrome (HUS) caused by Shiga toxins (Stxs) of enterohemorrhagic Escherichia coli (EHEC). We performed a comprehensive structural analysis of the Stx receptor glycosphingolipids (GSLs) globotriaosylceramide (Gb3Cer, Galα4alβ4Glcβ1Cer) and globotetraosylceramide (Gb4Cer, GalNAcβ3Galα4Galβ4Glcβ1Cer) and their distribution in lipid raft analogue detergent-resistant membranes (DRMs) and nonDRMs prepared from primary HRGECs...
May 23, 2017: Glycobiology
https://www.readbyqxmd.com/read/28535068/a-conformal-lithium-fluoride-protection-layer-on-three-dimensional-lithium-by-non-hazardous-gaseous-reagent-freon
#12
Dingchang Lin, Yayuan Liu, Wei Chen, Guangmin Zhou, Kai Liu, Bruce S Dunn, Yi Cui
Research on lithium (Li) metal chemistry has been rapidly gaining momentum nowadays not only because of the appealing high theoretical capacity, but also its indispensable role in the next-generation Li-S and Li-air batteries. However, two root problems of Li metal, namely high reactivity and infinite relative volume change during cycling, bring about numerous other challenges that impede its practical applications. In the past, extensive studies have targeted these two root causes by either improving interfacial stability or constructing a stable host...
May 23, 2017: Nano Letters
https://www.readbyqxmd.com/read/28535061/sitting-phase-monolayers-of-polymerizable-phospholipids-create-dimensional-molecular-scale-wetting-control-for-scalable-solution-based-patterning-of-layered-materials
#13
Shi Wah Choong, Shane R Russell, Jae Jin Bang, Justin K Patterson, Shelley A Claridge
The use of dimensionally ordered ligands on layered materials to direct local electronic structure and interactions with the environment promises to streamline integration into nanostructured electronic, optoelectronic, sensing, and nanofluidic interfaces. Substantial progress has been made in using ligands to control substrate electronic structure. Conversely, using the exposed face of the ligand layer to structure wetting and binding interactions, particularly with scalable solution- or spray-processed materials, remains a significant challenge...
May 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28535055/ionic-liquid-anion-controlled-nanoscale-gold-morphology-grown-at-a-liquid-interface
#14
Nakara Bhawawet, Jeremy B Essner, Durgesh Vinod Wagle, Gary A Baker
Two different ionic liquids comprising the tetrabutylphosphonium cation ([P4444]) paired with the strongly-coordinating anions 6-aminocaproate ([6-AC]) or taurinate ([tau]) were prepared and employed in an aqueous/organic liquid bilayer system to generate nanoscale gold by Au(OH)4- photoreduction. Generally, as the concentration of ionic liquid in the organic phase was increased, the resulting quasi-spherical gold nanoparticles reduced in size and presented less aggregation, leading to marked increases in the catalytic efficiency for 4-nitrophenol reduction using borohydride...
May 23, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28535053/the-origin-of-ion-pairing-and-redissociation-of-ionic-liquid
#15
Bong-Seop Lee, Shiang-Tai Lin
We address the possible occurrence of a minimum extent of dissociation (α) of ionic liquid (IL) in IL-solvent mixtures. This phenomenon, known as the re-dissociation of IL, is responsible for many interesting composition-dependent properties in such mixtures. A predictive thermodynamic model is developed to provide semi-quantitative description on the change of α with solvent concentration. It is found that the occurrence of minimum α coincides with the occurrence maximum mean activity coefficient of dissociated ions, indicating better solvation of free, dissociated ions both with decreasing and increasing solvent concentration...
May 23, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28535043/identification-and-quantification-of-acylated-homoserine-lactones-in-shewanella-baltica-the-specific-spoilage-organism-of-pseudosciaena-crocea-by-ultra-high-performance-liquid-chromatography-coupled-to-triple-quadrupole-mass-spectrometry
#16
Yanbo Wang, Xiaoxiao Zhang, Chong Wang, Linglin Fu, Yanyao Yi, Yan Zhang
Bacteria communicate with one another using chemical signal molecules called autoinducers, and the most prevalent autoinducers used by Gram-negative bacteria are N-acylated homoserine lactones (AHLs). However, AHLs are hardly detected in Shewanella baltica, the specific spoilage organism of Pseudosciaena crocea. In this study, we applied ultra-high-performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry (UHPLC-QqQ-MS/MS) to determine AHLs. This method enabled the quantification of up to 11 AHLs within 5...
May 23, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28535039/deposition-of-gold-nanotriangles-in-large-scale-close-packed-monolayers-for-x-ray-based-temperature-calibration-and-sers-monitoring-of-plasmon-driven-catalytic-reactions
#17
Ferenc Liebig, Radwan M Sarhan, Mathias Sander, Wouter A Koopman, Roman Schuetz, Matias Bargheer, Joachim Koetz
Anisotropic plasmonic particles such as gold nanotriangles have extraordinary structural, optical and physico-chemical properties. For many applications in different fields it is essential to prepare them in a chemically and physically stable, structurally well-defined manner, e.g., as large and uniform coverage on a substrate. We present a direct method for the large scale close-packed monolayer formation of edge-to-edge ordered, ultrathin crystalline gold nanotriangles on Si-wafers or quartz glass via the transfer of these asymmetric particles to the air-liquid interface after adding ethanol-toluene mixtures without any subsequent surface functionalization...
May 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28534980/a-proteomic-analysis-identifies-candidate-early-biomarkers-to-predict-ovarian-hyperstimulation-syndrome-in-polycystic-ovarian-syndrome-patients
#18
Lan Wu, Yazhou Sun, Jun Wan, Ting Luan, Qing Cheng, Yong Tan
Ovarian hyperstimulation syndrome (OHSS) is a potentially life‑threatening, iatrogenic complication that occurs during assisted reproduction. Polycystic ovarian syndrome (PCOS) significantly increases the risk of OHSS during controlled ovarian stimulation. Therefore, a more effective early prediction technique is required in PCOS patients. Quantitative proteomic analysis of serum proteins indicates the potential diagnostic value for disease. In the present study, the authors revealed the differentially expressed proteins in OHSS patients with PCOS as new diagnostic biomarkers...
May 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28534923/divergent-syntheses-of-iodinated-isobenzofuranones-and-isochromenones-by-iodolactonization-of-2-alkynylbenzoic-acids-in-ionic-liquids
#19
Raffaella Mancuso, Christian C Pomelli, Francesco Malafronte, Asif Maner, Nadia Marino, Cinzia Chiappe, Bartolo Gabriele
The regiochemical outcome of the iodolactonization of 2-alkynylbenzoic acids, carried out at 100 °C in ionic liquids (ILs) as unconventional solvents and with molecular iodine as the iodine source, in the absence of external bases, was found to be strongly dependent on the nature of the IL medium. In particular, while the use of N-ethyl-N-methylmorpholinium dicyanamide (Mor1,2N(CN)2) promoted the stereoselective formation of (E)-3-(iodomethylene)isobenzofuran-1(3H)-ones, through an anti-5-exo-dig cyclization route, the use of 1-ethyl-3-methylimidazolium ethyl sulfate (EmimEtSO4) tended to favor the 6-endo-dig cyclization mode, with preferential or selective formation of 4-iodo-1H-isochromen-1-ones...
May 23, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28534872/a-light-driven-artificial-flytrap
#20
Owies M Wani, Hao Zeng, Arri Priimagi
The sophistication, complexity and intelligence of biological systems is a continuous source of inspiration for mankind. Mimicking the natural intelligence to devise tiny systems that are capable of self-regulated, autonomous action to, for example, distinguish different targets, remains among the grand challenges in biomimetic micro-robotics. Herein, we demonstrate an autonomous soft device, a light-driven flytrap, that uses optical feedback to trigger photomechanical actuation. The design is based on light-responsive liquid-crystal elastomer, fabricated onto the tip of an optical fibre, which acts as a power source and serves as a contactless probe that senses the environment...
May 23, 2017: Nature Communications
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