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https://www.readbyqxmd.com/read/28927165/air-and-surface-sampling-method-for-assessing-exposures-to-quaternary-ammonium-compounds-using-liquid-chromatography-tandem-mass-spectrometry
#1
Ryan F LeBouf, Mohammed Abbas Virji, Anand Ranpara, Aleksandr B Stefaniak
This method was designed for sampling select quaternary ammonium (quat) compounds in air or on surfaces followed by analysis using ultraperformance liquid chromatography tandem mass spectrometry. Target quats were benzethonium chloride, didecyldimethylammonium bromide, benzyldimethyldodecylammonium chloride, benzyldimethyltetradecylammonium chloride, and benzyldimethylhexadecylammonium chloride. For air sampling, polytetrafluoroethylene (PTFE) filters are recommended for 15-min to 24-hour sampling. For surface sampling, Pro-wipe® 880 (PW) media was chosen...
July 1, 2017: Annals of Work Exposures and Health
https://www.readbyqxmd.com/read/28927140/itraq-coupled-2d-lc-ms-ms-analysis-of-cxcr7-transfected-papillary-thyroid-carcinoma-cells-a-new-insight-into-cxcr7-regulation-of-papillary-thyroid-carcinoma-progression-and-identification-of-potential-biomarkers
#2
Hengwei Zhang, Lei Yang, Zhangyi Liu, Chenxi Liu, Xuyong Teng, Lei Zhang, Bo Yin, Zhen Liu
Previous studies have demonstrated that C-X-C chemokine receptor type 7 (CXCR7) regulates papillary thyroid carcinoma (PTC) growth and metastasis; however, the molecular mechanisms underlying this regulation remain unclear. In the present study, the protein expression profiles of the PTC cell line GLAG-66 and GLAG-66 cells stably transfected with CXCR7 cDNA were analyzed and compared using isobaric tag for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927119/effect-of-glutamine-enriched-nutritional-support-on-intestinal-mucosal-barrier-function-mmp-2-mmp-9-and-immune-function-in-patients-with-advanced-gastric-cancer-during-perioperative-chemotherapy
#3
Juan Wang, Yanfen Li, Yuanling Qi
We studied the effects of glutamine-enriched nutritional support on intestinal mucosal barrier, matrix metalloproteinase (MMP)-2, MMP-9 and immune function during perioperative chemotherapy in patients with advanced gastric cancer. The study was conducted on 94 patients with advanced gastric cancer admitted from April 2015 to March 2016. They were randomly divided into observation and control groups, n=47. Control group was given basic nutritional support whereas glutamine-enriched nutritional support was given to patients in observation group...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927003/expanding-the-applicability-of-poly-ionic-liquids-in-solid-phase-microextraction-pyrrolidinium-coatings
#4
David J S Patinha, Liliana C Tomé, Mehmet Isik, David Mecerreyes, Armando J D Silvestre, Isabel M Marrucho
Crosslinked pyrrolidinium-based poly(ionic liquids) (Pyrr-PILs) were synthesized through a fast, simple, and solventless photopolymerization scheme, and tested as solid phase microextraction (SPME) sorbents. A series of Pyrr-PILs bearing three different alkyl side chain lengths with two, eight, and fourteen carbons was prepared, characterized, and homogeneously coated on a steel wire by using a very simple procedure. The resulting coatings showed a high thermal stability, with decomposition temperatures above 350 °C, excellent film stability, and lifetime of over 100 injections...
September 18, 2017: Materials
https://www.readbyqxmd.com/read/28926970/proteomic-characterization-of-armillaria-mellea-reveals-oxidative-stress-response-mechanisms-and-altered-secondary-metabolism-profiles
#5
Cassandra Collins, Rachel Hurley, Nada Almutlaqah, Grainne O'Keeffe, Thomas M Keane, David A Fitzpatrick, Rebecca A Owens
Armillaria mellea is a major plant pathogen. Yet, the strategies the organism uses to infect susceptible species, degrade lignocellulose and other plant material and protect itself against plant defences and its own glycodegradative arsenal are largely unknown. Here, we use a combination of gel and MS-based proteomics to profile A. mellea under conditions of oxidative stress and changes in growth matrix. 2-DE and LC-MS/MS were used to investigate the response of A. mellea to H₂O₂ and menadione/FeCl₃ exposure, respectively...
September 17, 2017: Microorganisms
https://www.readbyqxmd.com/read/28926907/research-on-the-quick-and-efficient-recovery-of-1-allyl-3-methylimidazolium-chloride-after-biomass-pretreatment-with-ionic-liquid-aqueous-alcohol-system
#6
Xiaocong Liang, Yan Fu, Jie Chang
Membrane-based methodology electrodialysis (ED) was employed for the quick and efficient recovery of ionic liquid AmimCl from the aqueous solutions after biomass pretreatment with AmimCl-aqueous methanol/ethanol systems. IL recovery performance was relatively stable as the variation of IL-alcohol systems employed in the pretreatment process. IL recovery ratio (R) of 66%-71%, IL recovery performance of specific energy consumption (Sp) of 429-467g/kwh and IL transport rate (Tr) of 5.3-8.2g/(m(2)min) were obtained by single ED treatment...
August 24, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28926867/diabetes-associated-biobanking-more-topical-than-ever
#7
Katharina Lydia Kynast, Nadine Volk, Thomas Fleming, Esther Herpel
The increasing prevalence of diabetes and its accompanying long-term complications, as well as the associated economic burden, calls for a rapid clinical translation of biomedical research to better valid the physiological relevance of the findings from basic research. To meet this condition, the Collaborative Research Center (CRC) 1118 has established the first nationwide diabetes-specific Biomaterialbank (BMB) that permanently preserves solid and liquid specimen retro- and prospectively at the Institute of Pathology and Department of Endocrinology of the University Hospital Heidelberg...
September 19, 2017: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/28926863/evaluation-of-cannabinoid-and-terpenoid-content-cannabis-flower-compared-to-supercritical-co2-concentrate
#8
Michelle Sexton, Kyle Shelton, Pam Haley, Mike West
A recent cannabis use survey revealed that 60% of cannabis users rely on smelling the flower to select their cannabis. Olfactory indicators in plants include volatile compounds, principally represented by the terpenoid fraction. Currently, medicinal- and adult-use cannabis is marketed in the United States with relatively little differentiation between products other than by a common name, association with a species type, and Δ-9 tetrahydrocannabinol/cannabidiol potency. Because of this practice, how terpenoid compositions may change during an extraction process is widely overlooked...
September 19, 2017: Planta Medica
https://www.readbyqxmd.com/read/28926841/beneficial-combination-of-lacosamide-with-retigabine-in-experimental-animals-an-isobolographic-analysis
#9
Jarogniew J Luszczki, Mirosław Zagaja, Barbara Miziak, Maria W Kondrat-Wrobel, Katarzyna Zaluska, Paula Wroblewska-Luczka, Piotr Adamczuk, Stanislaw J Czuczwar, Magdalena Florek-Luszczki
BACKGROUND/AIM: To isobolographically determine the types of interactions that occur between retigabine and lacosamide (LCM; two third-generation antiepileptic drugs) with respect to their anticonvulsant activity and acute adverse effects (sedation) in the maximal electroshock-induced seizures (MES) and chimney test (motor performance) in adult male Swiss mice. METHODS: Type I isobolographic analysis for nonparallel dose-response effects for the combination of retigabine with LCM (at the fixed-ratio of 1:1) in both the MES and chimney test in mice was performed...
September 20, 2017: Pharmacology
https://www.readbyqxmd.com/read/28926792/u-isotopes-distribution-in-the-lower-rhone-river-and-its-implication-on-radionuclides-disequilibrium-within-the-decay-series
#10
REVIEW
Mathilde Zebracki, Xavier Cagnat, Stéphanie Gairoard, Nicolas Cariou, Frédérique Eyrolle-Boyer, Béatrice Boulet, Christelle Antonelli
The large rivers are main pathways for the delivery of suspended sediments into coastal environments, affecting the biogeochemical fluxes and the ecosystem functioning. The radionuclides from (238)U and (232)Th-series can be used to understand the dynamic processes affecting both catchment soil erosion and sediment delivery to oceans. Based on annual water discharge the Rhone River represents the largest river of the Mediterranean Sea. The Rhone valley also represents the largest concentration in nuclear power plants in Europe...
September 16, 2017: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/28926717/multiple-metabolic-pathways-for-metabolism-of-l-tryptophan-in-i-fusarium-graminearum-i
#11
Kun Luo, Caro-Lyne DesRoches, Anne Johnston, Linda J Harris, Hui-Yan Zhao, Thérèse Ouellet
<i>Fusarium graminearum </i>is a plant pathogen that can cause the devastating cereal grain disease fusarium head blight (FHB) in temperate regions of the world. Previous studies have shown that <i>F. graminearum </i>can synthetize indole-3-acetic acid (auxin) using L-tryptophan (L-TRP)-dependent pathways. In the present study, we have taken a broader approach to examine the metabolism of L-TRP in <i>F. graminearum </i>liquid culture. Our results showed that <i>F. graminearum </i>was able to transiently produce the indole tryptophol when supplied with L-TRP...
September 19, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28926709/simulating-osmotic-equilibria-a-new-tool-to-calculate-activity-coefficients-in-concentrated-aqueous-salt-solutions
#12
Michael Bley, Magali Duvail, Philippe Guilbaud, Jean-Francois Dufrêche
Herein, a new theoretical method simulating osmotic equilibria and activities is presented, where a bulk liquid and its corresponding vapor phase are simulated with molecular dynamics using polarizable force fields. Time-averaged number density profiles based on the resulting trajectories provide the amount of evaporated molecules present in the vapor phase and consequently the vapor phase density. The activity of the solvent and the corresponding osmotic coefficient are determined by the vapor density at a certain solute concentration with respect to the reference vapor density of the pure solvent...
September 19, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28926701/forensic-sampling-and-analysis-from-a-single-substrate-for-surface-enhanced-raman-spectroscopy-followed-by-paper-spray-mass-spectrometry
#13
Patrick W Fedick, Brandon J Bills, Nicholas E Manicke, R Graham Cooks
Sample preparation is the most common bottle-neck in the analysis and processing of forensic evidence. Time-consuming steps in many forensic tests involve complex separations such as liquid and gas chromatography or various types of extraction techniques, typically coupled with mass spectrometry (e.g. LC-MS). Ambient ionization ameliorates these slow steps by reducing or even eliminating sample preparation. While some ambient ionization techniques have been adopted by the forensic community, there is significant resistance to discarding chromatography as most forensic analyses require both an identification and a confirmation technique...
September 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28926682/development-of-a-simple-and-valid-method-for-the-trace-determination-of-phthalate-esters-in-human-plasma-using-dispersive-liquid-liquid-microextraction-coupled-with-gc-ms
#14
Karim Ebrahim, Parinaz Poursafa, Mohammad Mehdi Amin
A new method was developed for the trace determination of phthalic acid esters in plasma using dispersive liquid-liquid microextraction and gas chromatography with mass spectrometry analysis. Plasma proteins were efficiently precipitated by trichloroacetic acid and then a mixture of chlorobenzene (as extraction solvent) and acetonitrile (as dispersive solvent) rapidly injected to clear supernatant using a syringe. After the centrifuging, chlorobenzene were sedimented in the bottom of the test tube. 1 μL of this sedimented phase was injected into the gas chromatograph for phthalic acid esters analysis...
September 19, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28926678/organic-polymer-based-monolithic-stationary-phases-with-incorporated-nanostructured-materials-for-hplc-and-cec
#15
REVIEW
Nisansala Ganewatta, Ziad El Rassi
This review article is concerned with the recent advances made in the field of organic polymer-based monoliths with incorporated nanostructured materials (NSMs) for use in liquid chromatography and capillary electrochromatography. It covers the pertinent literature published over the last 7-8 years with a total of 56 references. The present article has two distinct parts: one major part encompassing "traditional" organic polymer-based monoliths modified with NSMs and a minor part on cryogels modified with NSMs...
September 19, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28926579/cervical-screening-with-primary-hpv-testing-or-cytology-in-a-population-of-women-in-which-those-aged-33-years-or-younger-had-previously-been-offered-hpv-vaccination-results-of-the-compass-pilot-randomised-trial
#16
Karen Canfell, Michael Caruana, Val Gebski, Jessica Darlington-Brown, Stella Heley, Julia Brotherton, Dorota Gertig, Chloe J Jennett, Annabelle Farnsworth, Jeffrey Tan, C David Wrede, Philip E Castle, Marion Saville
BACKGROUND: Using primary human papillomavirus (HPV) testing for cervical screening increases detection of high-grade cervical intraepithelial neoplastic lesions and invasive cancer (cervical intraepithelial neoplasia grade 2+ [CIN2+]) compared to cytology, but no evaluation has been conducted in a population previously offered HPV vaccination. We aimed to assess colposcopy referral and CIN2+ detection rates for HPV-screened versus cytology-screened women in Australia's HPV-vaccinated population (by 2014, resident women ≤33 years had been age-eligible for HPV vaccination, with 3-dose uptake across age cohorts being about 50%-77%)...
September 2017: PLoS Medicine
https://www.readbyqxmd.com/read/28926513/pharmacokinetics-and-safety-profile-of-single-dose-administration-of-an-estrogen-receptor-%C3%AE-selective-phytoestrogenic-phytoserm-formulation-in-perimenopausal-and-postmenopausal-women
#17
Gerson Hernandez, Liqin Zhao, Adrian A Franke, Yu-Ling Chen, Wendy J Mack, Roberta D Brinton, Lon S Schneider
OBJECTIVE: Selected estrogen receptor β-selective phytoestrogen (phytoSERM), a preparation of genistein, daidzein, and S-equol, has an 83-fold selective affinity for estrogen receptor (ER) β, and may promote neuronal survival and estrogenic mechanisms in the brain without exerting feminizing activity in the periphery. The aim of this study was to assess the safety, tolerability, and single-dose pharmacokinetics of the phytoSERM formulation in perimenopausal and postmenopausal women...
September 18, 2017: Menopause: the Journal of the North American Menopause Society
https://www.readbyqxmd.com/read/28926322/vitamin-d-status-serum-lipid-concentrations-and-vitamin-d-receptor-vdr-gene-polymorphisms-in-familial-mediterranean-fever
#18
Turan Turhan, Halef Okan Doğan, Nihal Boğdaycioğlu, Nilnur Eyerci, Ahmet Omma, İsmail Sari, Ahmet Yeşilyurt, Yaşar Karaaslan
Vitamin D (VitD) is critical for the regulation of inflammatory processes, and VitD deficiency has been linked to several chronic inflammatory disorders. We aimed to investigate the concentrations of serum 25(OH)D3, lipid parameters, and three known VDR polymorphisms (BsmI, FokI, and TaqI) in patients with Familial Mediterranean fever (FMF), an autosomal recessive autoinflammatory disease. The study included 123 FMF patients and 105 controls. A total of 38 patients were in acute attacks at the time of investigation...
September 18, 2017: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28926300/an-updated-scheme-for-categorizing-foods-implicated-in-foodborne-disease-outbreaks-a-tri-agency-collaboration
#19
LaTonia C Richardson, Michael C Bazaco, Cary Chen Parker, Daniel Dewey-Mattia, Neal Golden, Karen Jones, Karl Klontz, Curtis Travis, Joanna Zablotsky Kufel, Dana Cole
BACKGROUND: Foodborne disease data collected during outbreak investigations are used to estimate the percentage of foodborne illnesses attributable to specific food categories. Current food categories do not reflect whether or how the food has been processed and exclude many multiple-ingredient foods. MATERIALS AND METHODS: Representatives from three federal agencies worked collaboratively in the Interagency Food Safety Analytics Collaboration (IFSAC) to develop a hierarchical scheme for categorizing foods implicated in outbreaks, which accounts for the type of processing and provides more specific food categories for regulatory purposes...
September 19, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28926293/the-development-of-a-novel-wound-healing-material-silk-elastin-sponge
#20
Shingo Kawabata, Katsuya Kawai, Satoshi Somamoto, Kazuo Noda, Yoshitaka Matsuura, Yoko Nakamura, Shigehiko Suzuki
Silk-elastin is a recombinant protein polymer with repeating units of silk and elastin blocks. This novel wound healing promoting material has the ability to self-assemble from a liquid to a gel. We have already reported that an aqueous solution of silk-elastin has the potential to accelerate wound healing; however, there are several problems in applying silk-elastin in the clinical setting. To solve these problems, we developed a silk-elastin sponge that is easy to use in the clinical setting. In the present study, we examined whether the wound healing effect of the silk-elastin sponge is equal to the aqueous solution of silk-elastin in vivo...
September 19, 2017: Journal of Biomaterials Science. Polymer Edition
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