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https://www.readbyqxmd.com/read/28732407/a-synergistic-combination-of-nutrient-reclamation-from-manure-and-resultant-hydrochar-upgradation-by-acid-supported-hydrothermal-carbonization
#1
Lichun Dai, Bo Yang, Hong Li, Furong Tan, Nengmin Zhu, Qili Zhu, Mingxiong He, Yi Ran, Guoquan Hu
Cattle manure was hydrothermally carbonized in acid solutions (0-2% HCl), then nutrient concentration in liquid product and physicochemical properties of hydrochar were characterized to investigate the effects of acid addition on hydrochar properties and nutrient recovery from manure. Results showed that hydrothermal carbonization (HTC) in 2% HCl extracted almost 100% and 63.38% of phosphorus and nitrogen, respectively; specifically, >90% of the extracted phosphorus was PO4-P in liquid from HTC with acid addition, and increasing amount of extracted nitrogen was NH4-N with increasing acid addition...
July 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28732399/analysis-of-emerging-organic-contaminants-in-water-fish-and-suspended-particulate-matter-spm-in-the-joint-danube-survey-using-solid-phase-extraction-followed-by-uhplc-ms-ms-and-gc-ms-analysis
#2
Robert Loos, Simona Tavazzi, Giulio Mariani, Gert Suurkuusk, Bruno Paracchini, Gunther Umlauf
In the third Joint Danube Survey (JDS3), emerging organic contaminants were analysed in the dissolved water phase of samples from the Danube River and its major tributaries. Analyses were performed using solid-phase extraction (SPE) followed by ultra-high-pressure liquid chromatography triple-quadrupole mass spectrometry (UHPLC-MS-MS) and gas chromatography-mass spectrometry (GC-MS). The polar organic compounds analysed by UHPLC-MS-MS were 1H-benzotriazole, methylbenzotriazoles, carbamazepine, 10,11-dihydro-10,11-dihydroxy-carbamazepine, diclofenac, sulfamethox-azole, 2,4-D (2,4-dichlorophenoxyacetic acid), MCPA (2-methyl-4-chlorophenoxyacetic acid), metolachlor, cybutryne (irgarol), terbutryn, DEET (N,N-diethyl-m-toluamide), and several perfluoroalkyl acids (C6-C9; C8=perfluorooctanoic acid (PFOA)) and perfluorooctansulfonic acid (PFOS)...
July 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28732394/surface-charge-effects-on-the-electro-orientation-of-insulating-boron-nitride-nanotubes-in-aqueous-suspension
#3
Semih Cetindag, Bishnu Tiwari, Dongyan Zhang, Yoke Khin Yap, Sangil Kim, Jerry W Shan
The alignment of hexagonal boron-nitride nanotubes (BNNTs) in aqueous KCl solutions under spatially uniform electric fields was examined experimentally, using direct optical visualization to probe the orientation dynamics of individual BNNTs for different electric-field frequencies. Different from most previously studied nanowires and nanotubes, BNNTs are wide-bandgap materials which are essentially insulating at room temperature. We analyze the electro-orientation of BNNTs in the general context of polarizable cylindrical particles in liquid suspensions, whose behavior can fall into different regimes, including alignment due to Maxwell-Wagner induced dipoles at high frequencies, and alignment due to fluid motion of the electrical double layer around the particles at lower frequencies...
May 29, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28732286/development-of-liquid-chromatographic-methods-for-enantioseparation-and-sensitive-detection-of-%C3%AE-adrenolytics-%C3%AE-2-agonists-in-human-plasma-using-a-single-enantiomer-reagent
#4
Poonam Malik, Ravi Bhushan
Enantioseparation of four commonly used β-adrenolytics (bisoprolol, salbutamol, and carvedilol, marketed as racemic mixtures) has been achieved by both TLC and RPHPLC via an indirect approach. A new chiral reagent, (S)-naproxen benzotriazole ester, was synthesized and it was characterized by UV, IR, (1)HNMR, elemental analysis and polarimetry. It was used to synthesize diastereomeric derivatives of the three β-adrenolytics under microwave irradiation. TLC separation of diastereomeric derivatives was achieved which were then isolated by preparative approach; these were characterized and were used as standard reference for determining absolute configuration of diastereomeric derivatives and for establishing validated HPLC method for enantioseparation and sensitive detection of the three β-adrenolytics in human plasma...
July 14, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28732228/a-facile-method-for-preparation-of-emulsion-using-the-high-gravity-technique
#5
Yan Li, Ran Andy Mei, Zhongqiang Yang
This study demonstrates a new process for preparation of oil-in-water (O/W) emulsion using the high gravity technique. This involves a mixture of oil and water that are passed through a rotating packed bed, under a high shear force, from which oil is emulsified into tiny droplets and subsequently dispersed in water. The process is cycled in order to break the droplets repeatedly and achieve an emulsion with small size and low polydispersity index (PDI). The advantage of the high gravity technique is that the emulsions with a desired size and polydispersity can be rapidly obtained by tuning experimental parameters, such as relative centrifugal force (RCF), cycle times (CT), liquid flow rate (VL) and surfactant concentration (Csurfactant)...
June 13, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28732213/comparison-of-blood-collection-tubes-from-three-different-manufacturers-for-the-collection-of-cell-free-dna-for-liquid-biopsy-mutation-testing
#6
Christina Alidousty, Danielle Brandes, Carina Heydt, Svenja Wagener, Maike Wittersheim, Stephan Christian Schäfer, Barbara Holz, Sabine Merkelbach-Bruse, Reinhard Büttner, Jana Fassunke, Anne Maria Schultheis
The improvement in sensitive techniques has allowed the detection of tumor-specific aberrations in circulating tumor (ct) DNA. Amplification-refractory mutation system PCR has been used for the analysis of ctDNA to detect resistance-causing mutations in the epidermal growth factor receptor gene (eg, EGFR T790M) in lung cancer patients. So far, Streck tubes have been widely used as standard blood collection tubes. Here, we compared blood collection tubes manufactured by Streck with tubes from Roche and Qiagen regarding their utility in stabilizing ctDNA in plasma samples...
July 18, 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28732139/advances-in-electrocatalysis-for-energy-conversion-and-synthesis-of-organic-molecules
#7
Yaovi Holade, Karine Servat, Sophie Tingry, Teko W Napporn, Hynd Remita, David Cornu, Kouakou Boniface Kokoh
The ubiquitous electrochemistry is expected to play a major role for reliable energy, and for production of sustainable fuels and chemicals. For organics-based electrocatalysis in an alkaline medium, the fundamental understandings at solid-liquid interfaces involve relatively complex mechanisms and performance descriptors (from the electrolyte and reaction intermediates), which undermine the roads towards advance and breakthroughs. Here, we review and diagnose recently designed strategies for the electrochemical conversion of organics into electricity or high added- value chemicals, and likely both...
July 21, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28732111/characterization-of-metabolic-pathways-and-absorption-of-sea-cucumber-saponins-holothurin-a-and-echinoside-a-in-vitro-and-in-vivo
#8
Shanshan Song, Lingyu Zhang, Jian Cao, Gao Xiang, Peixu Cong, Ping Dong, Zhaojie Li, Changhu Xue, Yong Xue, Yuming Wang
Sea cucumber saponins (SCSs) exhibit a wide spectrum of bioactivities, but their metabolic characteristics are not well elucidated. In this study, the metabolism of holothurin A (HA) and echinoside A (EA), 2 major saponins in sea cucumber, by gut microflora were investigated. First, we conducted an in vitro study, where in the SCSs were incubated with intestinal microflora and the metabolites were detected by high pressure liquid chromatography-high resolution mass spectrometry. We also conducted an in vivo study on rats, where in the intestinal contents, serum, urine, and feces were collected and evaluated after oral administration of SCSs...
July 21, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28732070/characterization-of-human-enterovirus71-virus-like-particles-used-for-vaccine-antigens
#9
Dandan Zhao, Bo Sun, Shiyang Sun, Bin Fu, Chuntian Liu, Dawei Liu, Yanfei Chu, Youlei Ma, Lu Bai, Yongge Wu, Yan Zhou, Weiheng Su, Ali Hou, Linjun Cai, Fei Xu, Wei Kong, Chunlai Jiang
Human enterovirus 71 (EV71) is a major causative pathogen of hand, foot and mouth disease (HFMD) and has caused outbreaks with significant mortality among young children in the Asia-Pacific region in recent years. Towards developing a vaccine for this disease, we have expressed and purified EV71 virus-like particles (VLPs), which resemble the authentic virus in appearance, capsid structure and protein sequence, from insect cells (Sf9) using a multistep chromatography process. We demonstrated intracellular localization of the VLPs in host cells by in situ immunogold detection, electron microscopy and immunofluorescence...
2017: PloS One
https://www.readbyqxmd.com/read/28732010/biofilm-imaging-in-porous-media-by-laboratory-x-ray-tomography-combining-a-non-destructive-contrast-agent-with-propagation-based-phase-contrast-imaging-tools
#10
Maxence Carrel, Mario A Beltran, Verónica L Morales, Nicolas Derlon, Eberhard Morgenroth, Rolf Kaufmann, Markus Holzner
X-ray tomography is a powerful tool giving access to the morphology of biofilms, in 3D porous media, at the mesoscale. Due to the high water content of biofilms, the attenuation coefficient of biofilms and water are very close, hindering the distinction between biofilms and water without the use of contrast agents. Until now, the use of contrast agents such as barium sulfate, silver-coated micro-particles or 1-chloronaphtalene added to the liquid phase allowed imaging the biofilm 3D morphology. However, these contrast agents are not passive and potentially interact with the biofilm when injected into the sample...
2017: PloS One
https://www.readbyqxmd.com/read/28732003/-diffuse-type-of-gastric-cancer-adenocarcinoma-in-10-years-old-boy-report-of-a-case
#11
Nery Romero, Ingrid Zegarra, Hugo Delgado
We report a rare case of diffuse type of gastric cancer with signet ring cells in 10 years old boy who was admitted with a 12 months history with weight loss, dysphagia to solids first and to liquids later, anorexia, fatigue, dizziness, vomiting and later, with pain in the left upper quadrant. On examination, he appeared pale, malnourished, with café-au- lait spots over the trunk and extremities. Laboratory tests showed; Hb 7.5 g, albumin 2.62 g, Thevenon positive on stools. Abdominal ultrasound examination showed periaortic masses and diffuse space occupying lesions in the liver...
April 2017: Revista de Gastroenterología del Perú: órgano Oficial de la Sociedad de Gastroenterología del Perú
https://www.readbyqxmd.com/read/28731758/noncontact-cohesive-swimming-of-bacteria-in-two-dimensional-liquid-films
#12
Ye Li, He Zhai, Sandra Sanchez, Daniel B Kearns, Yilin Wu
Bacterial swimming in confined two-dimensional environments is ubiquitous in nature and in clinical settings. Characterizing individual interactions between swimming bacteria in 2D confinement will help to understand diverse microbial processes, such as bacterial swarming and biofilm formation. Here we report a novel motion pattern displayed by flagellated bacteria in 2D confinement: When two nearby cells align their moving directions, they tend to engage in cohesive swimming without direct cell body contact, as a result of hydrodynamic interaction but not flagellar intertwining...
July 7, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28731736/enhancing-entropy-and-enthalpy-fluctuations-to-drive-crystallization-in-atomistic-simulations
#13
Pablo M Piaggi, Omar Valsson, Michele Parrinello
Crystallization is a process of great practical relevance in which rare but crucial fluctuations lead to the formation of a solid phase starting from the liquid. As in all first order first transitions, there is an interplay between enthalpy and entropy. Based on this idea, in order to drive crystallization in molecular simulations, we introduce two collective variables, one enthalpic and the other entropic. Defined in this way, these collective variables do not prejudge the structure into which the system is going to crystallize...
July 7, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28731711/identification-of-proteomic-features-to-distinguish-benign-pulmonary-nodules-from-lung-adenocarcinoma
#14
Simona G Codreanu, Megan D Hoeksema, Robbert J C Slebos, Lisa J Zimmerman, S M Jamshedur Rahman, Ming Li, Sheau-Chiann Chen, Heidi Chen, Rosana Eisenberg, Daniel C Liebler, Pierre P Massion
We hypothesized that distinct protein expression features of benign and malignant pulmonary nodules may reveal novel candidate biomarkers for the early detection of lung cancer. We performed proteome profiling by liquid chromatography-tandem mass spectrometry to characterize 34 resected benign lung nodules, 24 early stage untreated lung adenocarcinomas (ADCs) and biopsies of bronchial epithelium. Group comparisons identified 65 proteins that differentiate nodules from ADCs and normal bronchial epithelium, and 66 proteins that differentiate ADCs from nodules and normal bronchial epithelium...
July 21, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28731694/the-foliar-desiccators-glyphosate-carfentrazone-and-paraquat-affect-the-technological-and-chemical-properties-of-cowpea-grains
#15
Igor da Silva Lindemann, Gustavo Heinrich Lang, Jessica Fernanda Hoffmann, Cesar Valmor Rombaldi, Mauricio de Oliveira, Moacir Cardoso Elias, Nathan Levien Vanier
The effects of the use of glyphosate (GLY), glyphosate plus carfentrazone (GLY/CAR), and paraquat (PAR) as plant desiccators on the technological and chemical properties of cowpea grains were investigated. All studied desiccants provided lower cooking time to freshly harvested cowpea. However, the coat color of PAR- and GLY/CAR-treated cowpea was reddish than control treatment. Principal component analysis (PCA) from liquid chromatography-mass spectrometry (LC-MS) data sets showed a clear distinction among cowpea from the different treatments...
July 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28731691/singlet-oxygen-photophysics-in-liquid-solvents-converging-on-a-unified-picture
#16
Mikkel Bregnhøj, Michael Westberg, Boris F Minaev, Peter R Ogilby
Singlet oxygen, O2(a(1)Δg), the lowest excited electronic state of molecular oxygen, is an omnipresent part of life on earth. It is readily formed through a variety of chemical and photochemical processes, and its unique reactions are important not just as a tool in chemical syntheses but also in processes that range from polymer degradation to signaling in biological cells. For these reasons, O2(a(1)Δg) has been the subject of intense activity in a broad distribution of scientific fields for the past ∼50 years...
July 21, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28731682/cold-adaptation-of-triosephosphate-isomerase
#17
Johan Åqvist
The main problem for enzymes from psychrophilic species, that need to work near the freezing point of liquid water, is the exponential decay of reaction rates as the temperature is lowered. Cold-adapted enzymes have solved this problem by shifting the activation enthalpy-entropy balance for the catalyzed reaction compared to their mesophilic orthologs. To understand the structural basis of this universal feature it is necessary to examine pairs of such orthologous enzymes, with known 3D structures, at the microscopic level...
July 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28731579/residual-dynamic-and-risk-assessment-of-dimethomorph-in-swiss-chard-grown-in-two-different-sites
#18
Md Humayun Kabir, A M Abd El-Aty, Md Musfiqur Rahman, Hyung Suk Chung, Han Sol Lee, Mi-Ra Kim, Byung-Joon Chang, Jing Wang, Ho-Chul Shin, Jae-Han Shim
Residue analysis of dimethomorph in Swiss chard cultivated at two different locations under greenhouse conditions was conducted using high-performance liquid chromatography-ultraviolet detector (HPLC-UVD) and confirmed by tandem mass spectrometry (LC-MS/MS). The randomly collected samples (over 14 days) were extracted with acetonitrile and purified using a Florisil solid-phase extraction (SPE) cartridge. Linearity over a concentration range of 0.05-50.0 mg/L had an excellent coefficient of determination (R(2) ) = 0...
July 21, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28731534/internal-quality-control-in-an-academic-cytopathology-laboratory-for-the-introduction-of-a-new-reporting-system-for-endometrial-cytology
#19
Niki Margari, Abraham Pouliakis, Dionysios Aninos, Christos Meristoudis, Magdalini Stamataki, Ioannis Panayiotides, Petros Karakitsos
BACKGROUND: To evaluate reproducibility of a reporting system for endometrial cytology. METHODS: Cytologic slides from 49 patients, prepared via liquid based cytology, were blindly examined by five cytopathologists of various experience levels, applying a recently introduced reporting system as previously reported. The agreement among cytopathologists was evaluated via Kappa (κ) statistics and the Kendall's Coefficient of Variation (W); cytologic results were compared with the relevant histologic report...
July 21, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28731471/changes-in-nutrient-stoichiometry-elemental-homeostasis-and-growth-rate-of-aquatic-litter-associated-fungi-in-response-to-inorganic-nutrient-supply
#20
Vladislav Gulis, Kevin A Kuehn, Louie N Schoettle, Desiree Leach, Jonathan P Benstead, Amy D Rosemond
Aquatic fungi mediate important energy and nutrient transfers in freshwater ecosystems, a role potentially altered by widespread eutrophication. We studied the effects of dissolved nitrogen (N) and phosphorus (P) concentrations and ratios on fungal stoichiometry, elemental homeostasis, nutrient uptake and growth rate in two experiments that used (1) liquid media and a relatively recalcitrant carbon (C) source and (2) fungi grown on leaf litter in microcosms. Two monospecific fungal cultures and a multi-species assemblage were assessed in each experiment...
July 21, 2017: ISME Journal
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