keyword
MENU ▼
Read by QxMD icon Read
search

Mechanosensing

keyword
https://www.readbyqxmd.com/read/29115292/fibronectin-bound-%C3%AE-5%C3%AE-1-integrins-sense-load-and-signal-to-reinforce-adhesion-in-less-than-a-second
#1
Nico Strohmeyer, Mitasha Bharadwaj, Mercedes Costell, Reinhard Fässler, Daniel J Müller
Integrin-mediated mechanosensing of the extracellular environment allows cells to control adhesion and signalling. Whether cells sense and respond to force immediately upon ligand-binding is unknown. Here, we report that during adhesion initiation, fibroblasts respond to mechanical load by strengthening integrin-mediated adhesion to fibronectin (FN) in a biphasic manner. In the first phase, which depends on talin and kindlin as well as on the actin nucleators Arp2/3 and mDia, FN-engaged α5β1 integrins activate focal adhesion kinase (FAK) and c-Src in less than 0...
November 6, 2017: Nature Materials
https://www.readbyqxmd.com/read/29112867/mechanosensation-a-catch-bond-that-only-hooks-one-way
#2
Vinay Swaminathan, Gregory M Alushin, Clare M Waterman
Single-molecule force spectroscopy and modeling have revealed that the adhesion molecule vinculin and F-actin form a catch bond that is dependent on the direction of forces along the actin filament. This may underlie the mechanisms by which cells sense directional physical cues.
November 6, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/29107827/growth-mediated-plant-movements-hidden-in-plain-sight
#3
REVIEW
Stacey L Harmer, Christopher J Brooks
While fast plant movements are spectacular but rare, almost all plants exhibit relatively slow, growth-mediated tropic movements that are key to their survival in the natural world. In this brief review, we discuss recent insights into the molecular mechanisms underlying phototropism, gravitropism, hydrotropism, and autostraightening. Careful molecular genetic and physiological studies have helped confirm the importance of lateral auxin gradients in gravitropic and phototropic responses. However, auxin signaling does not explain all tropisms: recent work has shown that abscisic acid signaling mediates root hydrotropism and has implicated mechanosensing in autostraightening, the organ straightening process recently modeled as a proprioceptive response...
November 3, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/29107331/force-triggers-yap-nuclear-entry-by-regulating-transport-across-nuclear-pores
#4
Alberto Elosegui-Artola, Ion Andreu, Amy E M Beedle, Ainhoa Lezamiz, Marina Uroz, Anita J Kosmalska, Roger Oria, Jenny Z Kechagia, Palma Rico-Lastres, Anabel-Lise Le Roux, Catherine M Shanahan, Xavier Trepat, Daniel Navajas, Sergi Garcia-Manyes, Pere Roca-Cusachs
YAP is a mechanosensitive transcriptional activator with a critical role in cancer, regeneration, and organ size control. Here, we show that force applied to the nucleus directly drives YAP nuclear translocation by decreasing the mechanical restriction of nuclear pores to molecular transport. Exposure to a stiff environment leads cells to establish a mechanical connection between the nucleus and the cytoskeleton, allowing forces exerted through focal adhesions to reach the nucleus. Force transmission then leads to nuclear flattening, which stretches nuclear pores, reduces their mechanical resistance to molecular transport, and increases YAP nuclear import...
October 25, 2017: Cell
https://www.readbyqxmd.com/read/29094772/mechanosensitiviy-of-aged-muscle-stem-cells
#5
Heleen E Boers, Mohammad Haroon, Fabien Le Grand, Astrid D Bakker, Jenneke Klein-Nulend, Richard T Jaspers
During aging, skeletal muscle tissue progressively declines in mass, strength, and regenerative capacity. Decreased muscle stem cell (MuSC) number and impaired function might underlie the aging-related muscle wasting and impaired regenerative capacity. As yet, the search for factors that regulate MuSC fate and function has revealed several biochemical factors within the MuSC niche that may be responsible for the decline in MuSC regenerative capacity. This decline cannot be explained by environmental factors solely, as the MuSC potential to regenerate muscle tissue is not reversed by changing the biochemical MuSC niche composition...
November 2, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29081083/ionotropic-receptor-76b-is-required-for-gustatory-aversion-to-excessive-na-in-drosophila
#6
Min Jung Lee, Ha Yeon Sung, HyunJi Jo, Hyung-Wook Kim, Min Sung Choi, Jae Young Kwon, KyeongJin Kang
Avoiding ingestion of excessively salty food is essential for cation homeostasis that underlies various physiological processes in organisms. The molecular and cellular basis of the aversive salt taste, however, remains elusive. Through a behavioral reverse genetic screening, we discover that feeding suppression by Na(+)-rich food requires Ionotropic Receptor 76b (Ir76b) in Drosophila labellar gustatory receptor neurons (GRNs). Concentrated sodium solutions with various anions caused feeding suppression dependent on Ir76b...
October 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29079742/dual-biomembrane-force-probe-enables-single-cell-mechanical-analysis-of-signal-crosstalk-between-multiple-molecular-species
#7
Lining Ju, Yunfeng Chen, Kaitao Li, Zhou Yuan, Baoyu Liu, Shaun P Jackson, Cheng Zhu
Conventional approaches for studying receptor-mediated cell signaling, such as the western blot and flow cytometry, are limited in three aspects: 1) The perturbing preparation procedures often alter the molecules from their native state on the cell; 2) Long processing time before the final readout makes it difficult to capture transient signaling events (<1 min); 3) The experimental environments are force-free, therefore unable to visualize mechanical signals in real time. In contrast to these methods in biochemistry and cell biology that are usually population-averaged and non-real-time, here we introduce a novel single-cell based nanotool termed dual biomembrane force probe (dBFP)...
October 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29074777/second-messenger-mediated-tactile-response-by-a-bacterial-rotary-motor
#8
Isabelle Hug, Siddharth Deshpande, Kathrin S Sprecher, Thomas Pfohl, Urs Jenal
When bacteria encounter surfaces, they respond with surface colonization and virulence induction. The mechanisms of bacterial mechanosensation and downstream signaling remain poorly understood. Here, we describe a tactile sensing cascade in Caulobacter crescentus in which the flagellar motor acts as sensor. Surface-induced motor interference stimulated the production of the second messenger cyclic diguanylate by the motor-associated diguanylate cyclase DgcB. This led to the allosteric activation of the glycosyltransferase HfsJ to promote rapid synthesis of a polysaccharide adhesin and surface anchoring...
October 27, 2017: Science
https://www.readbyqxmd.com/read/29065178/intravital-imaging-of-osteocytes-in-mouse-calvaria-using-third-harmonic-generation-microscopy
#9
Danielle Tokarz, Richard Cisek, Marc N Wein, Raphaël Turcotte, Christa Haase, Shu-Chi A Yeh, Srinidhi Bharadwaj, Anthony P Raphael, Hari Paudel, Clemens Alt, Tzu-Ming Liu, Henry M Kronenberg, Charles P Lin
Osteocytes are the most abundant cell in the bone, and have multiple functions including mechanosensing and regulation of bone remodeling activities. Since osteocytes are embedded in the bone matrix, their inaccessibility makes in vivo studies problematic. Therefore, a non-invasive technique with high spatial resolution is desired. The purpose of this study is to investigate the use of third harmonic generation (THG) microscopy as a noninvasive technique for high-resolution imaging of the lacunar-canalicular network (LCN) in live mice...
2017: PloS One
https://www.readbyqxmd.com/read/29058683/cooperative-mechanosensitivity-and-allostery-of-focal-adhesion-clusters
#10
Darryl C W Foo, Eugene Terentjev
We analyse a role of cooperative interaction between neighbouring adhesion-mechanosensor complexes by constructing an Ising-like Hamiltonian describing the free energy of cell adhesion on a substrate as a lattice of 3-state mechanosensing sites involving focal adhesion kinase (FAK). We use Monte Carlo stochastic algorithm to find equilibrium configurations of these mechanosensors in two representative geometries: on a 1D ring representing the rim of a cell on flat surface, and a 2D bounded surface representing the whole area of cell contact with flat surface...
October 23, 2017: Physical Biology
https://www.readbyqxmd.com/read/29055011/unc-45a-promotes-myosin-folding-and-stress-fiber-assembly
#11
Jaakko I Lehtimäki, Aidan M Fenix, Tommi M Kotila, Giuseppe Balistreri, Lassi Paavolainen, Markku Varjosalo, Dylan T Burnette, Pekka Lappalainen
Contractile actomyosin bundles, stress fibers, are crucial for adhesion, morphogenesis, and mechanosensing in nonmuscle cells. However, the mechanisms by which nonmuscle myosin II (NM-II) is recruited to those structures and assembled into functional bipolar filaments have remained elusive. We report that UNC-45a is a dynamic component of actin stress fibers and functions as a myosin chaperone in vivo. UNC-45a knockout cells display severe defects in stress fiber assembly and consequent abnormalities in cell morphogenesis, polarity, and migration...
October 20, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/29052994/force-induced-calpain-cleavage-of-talin-is-critical-for-growth-adhesion-development-and-rigidity-sensing
#12
Mayur Saxena, Rishita Changede, James C Hone, Haguy Wolfenson, Michael P Sheetz
Cell growth depends upon formation of cell-matrix adhesions, but mechanisms detailing the transmission of signals from adhesions to control proliferation are still lacking. Here, we find that the scaffold protein talin undergoes force-induced cleavage in early adhesions to produce the talin rod fragment that is needed for cell cycle progression. Expression of non-cleavable talin blocks cell growth, adhesion maturation, proper mechanosensing, and the related property of EGF activation of motility. Further, the expression of talin rod in the presence of non-cleavable full-length talin rescues cell growth and other functions...
October 20, 2017: Nano Letters
https://www.readbyqxmd.com/read/29032920/cell-adhesion-mechanosensitivity-an-active-biological-process-comment-on-cellular-mechanosensing-of-the-biophysical-microenvironment-a-review-of-the-mathematical-models-of-biophysical-regulation-of-cell-responses-by-bo-cheng-et-al
#13
https://www.readbyqxmd.com/read/29027020/analysis-of-ca-2-response-of-osteocyte-network-by-three-dimensional-time-lapse-imaging-in-living-bone
#14
Tomoyo Tanaka, Mitsuhiro Hoshijima, Junko Sunaga, Takashi Nishida, Mana Hashimoto, Naoya Odagaki, Ryuta Osumi, Taiji Aadachi, Hiroshi Kamioka
Osteocytes form a three-dimensional (3D) cellular network within the mineralized bone matrix. The cellular network has important roles in mechanosensation and mechanotransduction related to bone homeostasis. We visualized the embedded osteocyte network in chick calvariae and observed the flow-induced Ca(2+) signaling in osteocytes using 3D time-lapse imaging. In response to the flow, intracellular Ca(2+) ([Ca(2+)]i) significantly increased in developmentally mature osteocytes in comparison with young osteocytes in the bone matrix...
October 12, 2017: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/29020917/deciphering-the-olfactory-repertoire-of-the-tiger-mosquito-aedes-albopictus
#15
Fabrizio Lombardo, Marco Salvemini, Carmine Fiorillo, Tony Nolan, Laurence J Zwiebel, José M Ribeiro, Bruno Arcà
BACKGROUND: The Asian tiger mosquito Aedes albopictus is a highly invasive species and competent vector of several arboviruses (e.g. dengue, chikungunya, Zika) and parasites (e.g. dirofilaria) of public health importance. Compared to other mosquito species, Ae. albopictus females exhibit a generalist host seeking as well as a very aggressive biting behaviour that are responsible for its high degree of nuisance. Several complex mosquito behaviours such as host seeking, feeding, mating or oviposition rely on olfactory stimuli that target a range of sensory neurons localized mainly on specialized head appendages such as antennae, maxillary palps and the mouthparts...
October 11, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29017047/cell-biology-capturing-formin-s-mechano-inhibition
#16
Dimitrios Vavylonis, Brandon G Horan
Formins polymerize actin filaments for the cytokinetic contractile ring. Using in vitro reconstitution of fission yeast contractile ring precursor nodes containing formins and myosin, a new study shows that formin-mediated polymerization is strongly inhibited upon the capture and pulling of actin filaments by myosin, a result that has broad implications for cellular mechanosensing.
October 9, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28993838/delta-opioid-receptor-expression-and-function-in-primary-afferent-somatosensory-neurons
#17
Amaury François, Grégory Scherrer
The functional diversity of primary afferent neurons of the dorsal root ganglia (DRG) generates a variety of qualitatively and quantitatively distinct somatosensory experiences, from shooting pain to pleasant touch. In recent years, the identification of dozens of genetic markers specifically expressed by subpopulations of DRG neurons has dramatically improved our understanding of this diversity and provided the tools to manipulate their activity and uncover their molecular identity and function. Opioid receptors have long been known to be expressed by discrete populations of DRG neurons, in which they regulate cell excitability and neurotransmitter release...
October 10, 2017: Handbook of Experimental Pharmacology
https://www.readbyqxmd.com/read/28988691/energetics-an-emerging-frontier-in-cellular-mechanosensing-reply-to-comments-on-cellular-mechanosensing-of-the-biophysical-microenvironment-a-review-of-mathematical-models-of-biophysical-regulation-of-cell-responses
#18
Bo Cheng, Min Lin, Guoyou Huang, Yuhui Li, Baohua Ji, Guy M Genin, Vikram S Deshpande, Tian Jian Lu, Feng Xu
How do cells can sense the substrate stiffness? Our recent review highlighted a range of theoretical models and simulations that have been proposed to answer this important question. In response to this review, three leading groups in the field noted some important omissions not only from our review itself but also from the field. These groups noted, correctly, that much of our understanding of cellular mechanosensing arises from models that take advantage of equilibrium thermodynamics, and that this is inappropriate because living cells are never in thermodynamic equilibrium...
September 29, 2017: Physics of Life Reviews
https://www.readbyqxmd.com/read/28978480/a-rhoa-fret-biosensor-mouse-for-intravital-imaging-in-normal-tissue-homeostasis-and-disease-contexts
#19
Max Nobis, David Herrmann, Sean C Warren, Shereen Kadir, Wilfred Leung, Monica Killen, Astrid Magenau, David Stevenson, Morghan C Lucas, Nadine Reischmann, Claire Vennin, James R W Conway, Alice Boulghourjian, Anaiis Zaratzian, Andrew M Law, David Gallego-Ortega, Christopher J Ormandy, Stacey N Walters, Shane T Grey, Jacqueline Bailey, Tatyana Chtanova, Julian M W Quinn, Paul A Baldock, Peter I Croucher, Juliane P Schwarz, Agata Mrowinska, Lei Zhang, Herbert Herzog, Andrius Masedunskas, Edna C Hardeman, Peter W Gunning, Gonzalo Del Monte-Nieto, Richard P Harvey, Michael S Samuel, Marina Pajic, Ewan J McGhee, Anna-Karin E Johnsson, Owen J Sansom, Heidi C E Welch, Jennifer P Morton, Douglas Strathdee, Kurt I Anderson, Paul Timpson
The small GTPase RhoA is involved in a variety of fundamental processes in normal tissue. Spatiotemporal control of RhoA is thought to govern mechanosensing, growth, and motility of cells, while its deregulation is associated with disease development. Here, we describe the generation of a RhoA-fluorescence resonance energy transfer (FRET) biosensor mouse and its utility for monitoring real-time activity of RhoA in a variety of native tissues in vivo. We assess changes in RhoA activity during mechanosensing of osteocytes within the bone and during neutrophil migration...
October 3, 2017: Cell Reports
https://www.readbyqxmd.com/read/28954860/receptor-mediated-cell-mechanosensing
#20
Yunfeng Chen, Lining Ju, Muaz Rushdi, Chenghao Ge, Cheng Zhu
Mechanosensing describes the ability of a cell to sense mechanical cues of its micro-environment, including not only all components of force, stress and strain but also substrate rigidity, topology and adhesiveness. This ability is crucial for the cell to respond to the surrounding mechanical cues and adapt to the changing environment. Examples of responses and adaptation include (de)activation, proliferation/apoptosis, and (de)differentiation. Receptor-mediated cell mechanosensing is a multi-step process that is initiated by binding of cell surface receptors to their ligands on the extracellular matrix or the surface of adjacent cells...
September 27, 2017: Molecular Biology of the Cell
keyword
keyword
117817
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"