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Dombrock blood group system

Harita Gogri, Pranali Pitale, Manisha Madkaikar, Swati Kulkarni
BACKGROUND: Haemagglutination has been the gold standard for defining the blood group status. However, these tests depend upon the availability of specific and reliable antisera. Potent antisera for extended phenotyping are very costly, weakly reacting or available in limited stocks and unavailable for some blood group systems like Indian, Dombrock, Coltan, Diego etc. The Indian blood group system consists of two antithetical antigens, Ina and Inb . The Ina /Inb polymorphism arises from 252C > G missense mutation in the CD44 gene...
April 11, 2018: Transfusion and Apheresis Science
Carolina B Bub
Dithiothreitol (DTT), a reducing reagent, has multiple applications in blood bank testing. DTT disrupts the bridging of the disulfide bonds between amino acid residues necessary for structural conformation of some proteins and the bonds holding an IgM molecule in the pentameric formation. DTT treatment of red blood cells (RBCs) can denature or modify certain blood group antigens-in particular, those in the Kell, Lutheran, YT, JMH, LW, Cromer, Indian, Dombrock, and Knops systems-and prevent recognition by the corresponding antibodies...
December 2017: Immunohematology
Yanli Ji, Jizhi Wen, Barbera Veldhuisen, Lonneke Haer-Wigman, Zhen Wang, Martin Lodén-van Straaten, Ling Wei, Guangping Luo, Yongshui Fu, C Ellen van der Schoot
BACKGROUND: Genotyping platforms for common red blood cell (RBC) antigens have been successfully applied in Caucasian and black populations but not in Chinese populations. In this study, a genotyping assay based on multiplex ligation-dependent probe amplification (MLPA) technology was applied in a Chinese population to validate the MLPA probes. Subsequently, the comprehensive distribution of 17 blood group systems also was obtained. STUDY DESIGN AND METHODS: DNA samples from 200 Chinese donors were extracted and genotyped using the blood-MLPA assay...
February 2017: Transfusion
Yun Ji Hong, Yousun Chung, Sang Mee Hwang, Jeong Su Park, Jeong-Ran Kwon, Young Sill Choi, Jun Nyun Kim, Dong Han Lee, So-Yong Kwon, Nam-Sun Cho, Eun Young Song, Kyoung Un Park, Junghan Song, Kyou Sup Han
It is often difficult for standard blood banks in Korea to supply adequate amounts of blood for patients with rare phenotype. Moreover, the definition of a blood in need is ambiguous, and much remains to be learned. In this study, we determined the prevalence of various red blood cell (RBC) antigens from a donor viewpoint and estimated the demand for specific antigen-negative blood from a patient viewpoint. Our data will aid the establishment of a Rare Blood Program in Korea (KRBP). RBC genotyping of 419 blood donors was performed using a Lifecodes RBC/RBC-R typing kit (Immucor, Norcross, GA)...
May 2016: Annals of Hematology
Thomas Braschler, Cora Alexandra Vökt, Hein Hustinx, Thierry Peyrard, Laura Infanti, Andreas Buser, Andreas Holbro
BACKGROUND: Holley (Hy) is a high-incidence antigen of the Dombrock blood group system (ISBT 014), present in almost 100% of most populations and more than 99% of Blacks. Since anti-Hy is an extremely rare antibody, data on its clinical relevance and in particular on a possible hemolytic disease of the fetus and newborn (HDFN) are scarce. CASE REPORT: The pregnant patient underwent two autologous whole blood collections at weeks 17 and 19 of gestation with cryopreservation...
March 2015: Transfusion Medicine and Hemotherapy
T Onodera, H Tsuneyama, K Ogasawara, K Isa, M Satake, K Tadokoro, M Uchikawa
The Dombrock blood group system consists of two antithetical antigens, Do(a) (DO1) and Do(b) (DO2), and seven high-prevalence antigens, Gy(a) (DO3), Hy (DO4), Jo(a) (DO5), DOYA (DO6), DOMR (DO7), DOLG (DO8) and DOLC (DO9). Do(a) /Do(b) polymorphism is associated with c.793A>G (p.Asn265Asp) in exon 2 of the DO (ART4) gene, and the corresponding alleles are named DO*01 and DO*02. The rare Donull or Gy(a-) phenotype lacks all Dombrock antigens, and the DO null alleles vary with both DO*01 and DO*02 backgrounds...
August 2015: Vox Sanguinis
Fabiana Chagas Camargos Piassi, Silvana Maria Eloi Santos, Lilian Maria de Castilho, Wilson Baleotti Júnior, Rodrigo Buzinaro Suzuki, Débora Moura da Cunha
BACKGROUND: Dombrock blood group system genotyping has revealed various rearrangements of the Dombrock gene and identified new variant alleles in Brazil (i.e., DO*A-SH, DO*A-WL and DO*B-WL). Because of the high heterogeneity of the Brazilian population, interregional differences are expected during the investigation of Dombrock genotypes. OBJECTIVE: The present study aims to determine the frequencies of Dombrock genotypes in blood donors from Minas Gerais and compare the frequencies of the HY and JO alleles to those of another population in Brazil...
2013: Revista Brasileira de Hematologia e Hemoterapia
S Mazières, S A Temory, H Vasseur, P Gallian, J Di Cristofaro, J Chiaroni
BACKGROUND AND OBJECTIVES: Blood incompatibility arises from individual and ethnic differences in red blood cell (RBC) antigen profiles. This underlines the importance of documenting RBC antigen variability in various ethnic groups. Central Asia is an area with a long and complex migratory history. The purpose of this article is to describe key antigen frequencies of Afghan ethnic groups in the Hindu-Kush region of Afghanistan as a basis for improving blood transfusion practices in that area...
June 2013: Transfusion Medicine
Wilson Baleotti, Rodrigo Buzinaro Suzuki, Milena Polotto, Marcelo Ortega Ruiz, Antonio Fabron, Lilian Castilho
BACKGROUND: Determination of the molecular basis underlying the antigens in the Dombrock blood group system has shown various rearrangements between the alleles associated with DO(*) A and DO(*) B. Based on this, we employed a PCR-based strategy to screen DO alleles (DO(*) A, DO(*) B, HY(*) 1, HY(*) 2 and JO) in Brazilians. METHODS: We tested DNA of 278 Brazilian blood donors by PCR-RFLP on plates of 96 wells to determine the 793A/G (DO(*) A/DO(*) B), 323G/T (HY), 350C/T (JO) and 898C/G (HY(*) 1/HY(*) 2) single nucletide polymorphisms...
2011: Journal of Clinical Laboratory Analysis
N M Burton, G Daniels
The red cell surface membranes contain a large variety of proteins, many of which express blood group activity as a result of variation in their oligosaccharide or amino acid sequences. To understand the nature of the blood group epitopes, the functions of the proteins that express them and their relationship to each other, computer modelling has been employed to provide predictions of their structures. Modelling is an excellent method of first resort when experimental structural data for the protein of interest are absent or incomplete...
January 2011: Vox Sanguinis
C Lomas-Francis, M E Reid
The Dombrock blood group system (Do) consists of two antithetical antigens (Do(a) and Do(b)) and five antigens of high prevalence (Gy(a), Hy, Jo(a), DOYA, and DOMR). Do antigens are carried on the Dombrock glycoprotein, which is attached to the RBC membrane via glycosylphosphatidylinositol linkage. The gene (DO, ART4) encoding the Do glycoprotein, located on the short arm of chromosome 12, has been cloned and sequenced, allowing the molecular basis of the various Do phenotypes to be determined. Do(a) and Do(b) have a prevalence that makes them useful as genetic markers; however, the paucity of reliable anti-Do(a) and anti-Do(b) has prevented this potential from being realized...
2010: Immunohematology
Flávia Pinheiro S Costa, Kim Hue-Roye, Laima Sausais, Randall W Velliquette, Eliete Da Costa Ferreira, Christine Lomas-Francis, Marion E Reid
BACKGROUND: The Dombrock (Do) blood group system consists of six distinct antigens: Do(a) , Do(b) , Gy(a) , Hy, Jo(a) , and DOYA. Our finding of a pregnant patient whose red blood cells (RBCs) were Hy+ but whose serum contained an apparent alloanti-Hy suggested the presence of a seventh antigen and prompted this study. STUDY DESIGN AND METHODS: Standard hemagglutination and polymerase chain reaction-based methods were used throughout. RESULTS: The patient's RBCs typed as Do(a-b+(w) ), Gy(a+(w) ), Hy+(w) , Jo(a+(w) ), and DOYA+(w) ...
September 2010: Transfusion
M H Tian, G R Halverson
Anti-Ok(a) was first described by Morel and Hamilton in 1979. The Ok(a) antigen has a very high incidence, and only eight probands that are Ok(a-) have been found; all are of Japanese heritage. In this study,we describe the generation and characterization of three novel monoclonal antibodies (Mabs), MIMA-25, MIMA-144, and MIMA-149. The reactivity of these three Mabs was compared with the original human polyclonal anti-Ok(a). Mice were immunized with transfected HEK cells to induce an immune response, and the spleen B lymphocytes were fused with mouse myeloma X63-Ag8...
2009: Immunohematology
Beate Mayer, Nicole Thornton, Salih Yürek, Dwane Wylie, Kim Hue-Roye, Joyce Poole, Thilo Bartolmäs, Abdulgabar Salama, Christine Lomas-Francis, Randall W Velliquette, Karina Yazdanbakhsh, Marion E Reid
BACKGROUND: The Dombrock (Do) blood group system consists of five distinct antigens: Do(a), Do(b), Gy(a), Hy, and Jo(a). Our finding of a patient whose plasma contained a Do-related alloantibody suggested the presence of a sixth antigen. STUDY DESIGN AND METHODS: Standard hemagglutination, flow cytometry, and polymerase chain reaction (PCR)-based methods were used throughout. Protein homology modeling was used to map the amino acid change on the protein structure...
June 2010: Transfusion
L Castilho, W Baleotti, E Tossas, K Hue-Roye, K R Ribeiro, C Lomas-Francis, D Charles-Pierre, M E Reid
Because of the scarcity of anti-Hy and anti-Jo(a), hemagglutination typing for the Dombrock blood group system antigens, Hy and Jo(a), is not feasible. The molecular bases associated with these antigens have been determined, making it possible to distinguish HY and JO from wild-type DO. This provides a tool to predict the probable phenotype of patients and to screen for antigen-negative donors. PCR-RFLP assays and a microchip assay were used to determine the frequency of HY and JO alleles in donors from Brazil and New York...
2008: Immunohematology
Sylvie Chapel-Fernandes, Isabelle Callebaut, Gregory R Halverson, Marion E Reid, Pascal Bailly, Jacques Chiaroni
BACKGROUND: Since variant alleles in the Dombrock (DO) blood group system are common in Africans, DNA typing of DO alleles in an uninvestigated Congolese Teke ethnic group was performed. STUDY DESIGN AND METHODS: DO exons were polymerase chain reaction amplified, using genomic DNA extracted from blood samples, and sequenced. Membrane expression in K562 cells transduced with DO-cDNAs using lentiviral vectors was studied by flow cytometry. Amino acid changes were mapped on the protein structure, predicted by homology modeling...
August 2009: Transfusion
K R Ribeiro, M H Guarnieri, D C da Costa, F F Costa, J Pellegrino, L Castilho
BACKGROUND: Blood samples from patients with sickle cell disease (SCD) present to transfusion service with numerous antibodies, making the searching for compatible red blood cells (RBC) a challenge. To overcome this problem we developed an effective strategy to meet needs of supplying RBC-compatible units to SCD patients using DNA arrays. METHODS: We selected DNA samples from 144 SCD patients with multiple (receiving > 5 units) transfusions previously phenotyped for ABO, Rh(D, C, c, E, e), K1, Fy(a) and Jk(a)...
August 2009: Vox Sanguinis
Mhammed Touinssi, Jacques Chiaroni, Anna Degioanni, Thomas Granier, Olivier Dutour, Pascal Bailly, Frédéric Bauduer
The Basques demonstrate peculiar characteristics regarding blood group systems. Although ABO, Rhesus, and Duffy have been extensively studied in this population, the distribution of other groups remains largely unknown. Therefore, we evaluated the frequency of less-explored- or still noninvestigated blood groups using DNA-based assays and interpreted these data in the view of population genetics. Polymorphisms of KEL (Kell), SLCA14A1 (Kidd), GYPA/GYPB (MNS), ART4 (Dombrock), AQP1 (Colton), and ACHE (Yt) blood group genes were determined from a sample of more than 100 autochthonous French Basques using allele-specific primer PCR (PCR-ASP) methods...
May 2008: American Journal of Human Biology: the Official Journal of the Human Biology Council
Connie Westhoff, Sunitha Vege, Karina Yazdanbakhsh, Dwane Wylie, Mohammad Razib, Kim Hue-Roye, Gregory Halverson, Sandy Read, Elizabeth Whiteoak, Pam Nickle, Joan Maurer, Donna Kavitsky, Sandra Nance, Marion E Reid
BACKGROUND: The gene polymorphisms responsible for the antigens Doa, Dob, Hy, and Joa in the Dombrock (Do) blood group system have been identified. Four different mutations have been reported to cause the Dombrock null [Gy(a-)] phenotype. These include splice mutations, an eight-nucleotide deletion, and insertion of a stop codon. Here a Dombrock null caused by a single-amino-acid substitution in the full-length protein is reported. STUDY DESIGN AND METHODS: DOA and DOB were determined by polymerase chain reaction-restriction fragment length polymorphism, and DO (ART4) exons and flanking regions were sequenced from genomic DNA...
August 2007: Transfusion
Markus T Rojewski, Hubert Schrezenmeier, Willy A Flegel
The proteins of blood group systems are expressed on red blood cells (RBC) by definition. We searched nucleotide databases of human expressed sequence tags (EST) to collate the distribution of 22 distinct membrane proteins in cells and tissues other than RBC. The documented blood group genes are: MNS, Rh, Lutheran, Kell, Duffy, Kidd, Diego, Yt, Xg, Scianna, Dombrock, Colton, Landsteiner-Wiener, Kx, Gerbich, Cromer, Knops, Indian, Ok, Raph, John-Milton-Hagen and Gill. The genes were grouped according to their overall and their relative expression in embryo and adults...
August 2006: Transfusion and Apheresis Science
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