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Lutheran blood group system

Krzysztof Mikołajczyk, Radosław Kaczmarek, Marcin Czerwiński
Transcription factor EKLF (Erythroid Krüppel-Like Factor) belongs to the group of Krüppellike factors, which regulate proliferation, differentiation, development and apoptosis of mammalian cells. EKLF factor is present in erythroid cells, where it participates in regulation of hematopoiesis, expression of genes encoding transmembrane proteins (including blood group antigens), and heme biosynthesis enzymes. It is also a key factor in downregulation of γ-globins and activation of β-globin gene expression...
October 6, 2016: Postȩpy Higieny i Medycyny Doświadczalnej
Umar Kangiwa, Obike Ibegbulam, Sunday Ocheni, Anazoeze Madu, Ndakosu Mohammed
BACKGROUND AND STUDY OBJECTIVES: Blood transfusion is central in the prevention and treatment of certain chronic complications of sickle cell disease. It is indispensible in correcting anaemias as well as in the practice of exchange blood transfusion. These gains are largely limited by formation of allo-antibodies. Several studies demonstrated varying frequencies of allo-immunization in various patient groups. The effect of the racial differences between the donor and recipient pool, which has been subsumed in this study, has continuously created a confounding effect on the results of previous studies...
2015: Biomarker Research
Andrew J Gentles, Scott V Bratman, Luke J Lee, Jeremy P Harris, Weiguo Feng, Ramesh V Nair, David B Shultz, Viswam S Nair, Chuong D Hoang, Robert B West, Sylvia K Plevritis, Ash A Alizadeh, Maximilian Diehn
BACKGROUND: Accurate survival stratification in early-stage non-small cell lung cancer (NSCLC) could inform the use of adjuvant therapy. We developed a clinically implementable mortality risk score incorporating distinct tumor microenvironmental gene expression signatures and clinical variables. METHODS: Gene expression profiles from 1106 nonsquamous NSCLCs were used for generation and internal validation of a nine-gene molecular prognostic index (MPI). A quantitative polymerase chain reaction (qPCR) assay was developed and validated on an independent cohort of formalin-fixed paraffin-embedded (FFPE) tissues (n = 98)...
October 2015: Journal of the National Cancer Institute
Angela Belsito, Dario Costa, Carmela Fiorito, Gustavo De Iorio, Amelia Casamassimi, Silverio Perrotta, Claudio Napoli
BACKGROUND AND OBJECTIVES: Although minor erythrocyte antigens are not considered clinically significant in sporadic transfusions, they may be relevant for multi-transfusion patients. When serological assay is not conceivable, molecular genotyping allows predicting the red blood cell phenotype, extending the typing until minor blood groups. The aim of this study was to evaluate the utility of blood group genotyping and compare the molecular typing of erythrocyte antigens with the established serological methods...
February 2015: Transfusion and Apheresis Science
Jamie L Maciaszek, Kostyantyn Partola, Jing Zhang, Biree Andemariam, George Lykotrafitis
Single-cell force spectroscopy (SCFS), an atomic force microscopy (AFM)-based assay, enables quantitative study of cell adhesion while maintaining the native state of surface receptors in physiological conditions. Human healthy and pathological red blood cells (RBCs) express a large number of surface proteins which mediate cell-cell interactions, or cell adhesion to the extracellular matrix. In particular, RBCs adhere with high affinity to subendothelial matrix laminin via the basal cell adhesion molecule and Lutheran protein (BCAM/Lu)...
December 18, 2014: Journal of Biomechanics
Divjot S Lamba, Kshitija Mittal, Tanvi Sood, Ravneet Kaur Bedi, Paramjit Kaur, Gagandeep Kaur
Life-long red blood cell (RBC) transfusions remain the main treatment for severe thalassemia. We hereby report a case of anti S and anti Lu(a) in a β-thalassemia major patient detected incidentally on antibody screening. The patient was a known case of β-thalassemia major and was on regular blood transfusion every 3 weeks from the institute from the age of 6 months. Subsequently, on one occasion, patient's crossmatch was compatible despite positive antibody screen using microcolumn gel technique. Autocontrol and direct antiglobulin test were negative on microcolumn gel...
October 2014: Transfusion and Apheresis Science
Yamato Kikkawa, Takahiro Miwa, Naoki Tanimizu, Yuichi Kadoya, Takaho Ogawa, Fumihiko Katagiri, Kentaro Hozumi, Motoyoshi Nomizu, Toru Mizuguchi, Koichi Hirata, Toshihiro Mitaka
Lutheran (Lu), an immunoglobulin superfamily transmembrane receptor, is also known as basal cell adhesion molecule (B-CAM). Lu/B-CAM is a specific receptor for laminin α5, a subunit of laminin-511 (LM-511) that is a major component of basement membranes in various tissues. Our previous study showed that Lu/B-CAM was cleaved by MT1-MMP and released from cell surfaces. In this study we examined the soluble Lu/B-CAM in culture media and in plasma of mice bearing HuH-7 hepatocellular carcinoma (HCC) cells and patients with HCC...
October 15, 2014: Experimental Cell Research
Chen Wang, Qin Li, Zhonghui Guo, Luyi Ye, Ziyan Zhu
OBJECTIVE: To study the frequency of rare blood group Lu(a-b-) phenotype in a population from Shanghai region, and to explore the molecular basis of Lu(a-b-) by detecting the Lu and Lu relative mediator gene EKLF/KLF1. METHODS: Donors from Shanghai region were screened for Lutheran blood group by monoclonal anti-Lub using serological methods. Individuals with Lu(b-) were determined Lua, P1 and i antigens. Fifteen exons of the LU gene and 3 exons of the EKLF/KLF1 gene for the identified Lu(a-b-) samples were amplified and sequenced...
April 2014: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
Manoj A Kahar, Rajnikant D Patel
BACKGROUND: This is the first study on phenotype frequencies of various blood group systems in blood donors of south Gujarat, India using conventional tube technique. MATERIAL AND METHODS: A total of 115 "O" blood group donors from three different blood banks of south Gujarat were typed for D, C, c, E, e, K, Jk(a), Le(a), Le(b), P1, M, and N antigens using monoclonal antisera and k, Kp(a), Kp(b), Fy(a),Fy(b), Jk(b), S,s, Lu(a), and Lu(b) antigens were typed using polyclonal antisera employing Indirect Antiglobulin Test...
January 2014: Asian Journal of Transfusion Science
Jamie L Maciaszek, Biree Andemariam, Krithika Abiraman, George Lykotrafitis
Human normal and sickle red blood cells (RBCs) adhere with high affinity to the alpha5 chain of laminin (LAMA5) via the basal cell adhesion molecule/Lutheran (BCAM/Lu) receptor, which is implicated in vasoocclusive episodes in sickle cell disease and activated through the cyclic adenosine monophosphate (cAMP) signaling pathway. However, the effect of the cAMP pathway on the expression of active BCAM/Lu receptors at the single-molecule level is unknown. We established an in vitro technique, based on atomic force microscopy, which enables detection of single BCAM/Lu proteins on the RBC surface and measures the unbinding force between BCAM/Lu and LAMA5...
March 18, 2014: Biophysical Journal
Vicky Chaar, Sandrine Laurance, Claudine Lapoumeroulie, Sylvie Cochet, Maria De Grandis, Yves Colin, Jacques Elion, Caroline Le Van Kim, Wassim El Nemer
Vaso-occlusive crises are the main acute complication in sickle cell disease. They are initiated by abnormal adhesion of circulating blood cells to vascular endothelium of the microcirculation. Several interactions involving an intricate network of adhesion molecules have been described between sickle red blood cells and the endothelial vascular wall. We have shown previously that young sickle reticulocytes adhere to resting endothelial cells through the interaction of α4β1 integrin with endothelial Lutheran/basal cell adhesion molecule (Lu/BCAM)...
April 18, 2014: Journal of Biological Chemistry
Nevenka B Bujandrić, Jasmina N Grujić, Mirjana M Krga-Milanović
INTRODUCTION: Red blood cell antibodies may cause a positive result of pre-transfusion blood compatibility testing (crossmatch test). It can be a problem to provide suitable blood units for patients with clinically significant antibodies to high-frequency antigens as well as for those with multiple alloantibody specificities. This study was aimed at identifying transfused patients in the population of South-Backa who had developed clinically significant red blood cell alloantibodies. MATERIAL AND METHODS: We analyzed the records of crossmatch results and antibody screening performed at the Blood Transfusion Institute of Vojvodina during 2012...
November 2013: Medicinski Pregled
Mélanie Franco, Caroline Le Van Kim
No abstract text is available yet for this article.
December 2013: Médecine Sciences: M/S
Hirotada Akiyama, Yoshimasa Iwahana, Mikiya Suda, Atsunori Yoshimura, Hiroyuki Kogai, Ai Nagashima, Hiroko Ohtsuka, Yuko Komiya, Fumio Tashiro
Basal cell adhesion molecule (BCAM), known to be a splicing variant of Lutheran glycoprotein (LU), is an immunoglobulin superfamily membrane protein that acts as a laminin α5 receptor. The high affinity of BCAM/LU for laminin α5 is thought to contribute to the pathogenesis of sickle red blood cells and to various developmental processes. However, the function of BCAM in carcinogenesis is poorly understood. Based on microarray expression analysis, we found that BCAM was one of the target genes of the oncogenic 14-3-3β-FBI1/Akirin2 complex, which acts as a transcriptional repressor and suppresses MAPK phosphatase-1 gene expression...
2013: PloS One
Yamato Kikkawa, Takaho Ogawa, Ryo Sudo, Yuji Yamada, Fumihiko Katagiri, Kentaro Hozumi, Motoyoshi Nomizu, Jeffrey H Miner
Cell-matrix interactions are critical for tumor cell migration. Lutheran (Lu), also known as basal cell adhesion molecule (B-CAM), competes with integrins for binding to laminin α5, a subunit of LM-511, a major component of basement membranes. Here we show that the preferential binding of Lu/B-CAM to laminin α5 promotes tumor cell migration. The attachment of Lu/B-CAM transfectants to LM-511 was slightly weaker than that of control cells, and this was because Lu/B-CAM disturbed integrin binding to laminin α5...
October 25, 2013: Journal of Biological Chemistry
G Ramis, L Martínez-Alarcon, J J Quereda, A Mrowiec, C Funes, A Ríos, P Ramírez, A Muñoz, M J Majado
Some biomedical research procedures, such as organ xenotransplantation, usually require intensive hemotherapy. Knowledge of the whole phenotype of blood donor and graft could be useful in the field of xenotransplantation. Human and simian-type categories of blood groups have been established and they can be tested by standard methods used for human blood grouping. The aim of this work was to study the incidence of non-ABO blood group systems in different species of non-human primates, which are employed in biomedical research...
April 2013: Laboratory Animals
K Hue-Roye, M E Reid
The Lutheran blood group system currently consists of 20 antigens that have been assigned ISBT numbers. Of these, all but LU7 have been associated with one or more nucleotide changes in LU. The purpose of this study was to determine the molecular basis associated with the LU:-7 phenotype. We obtained a stored sample from one proband with this phenotype and sequenced LU. Using genomic DNA, exons 1 through 15, and their flanking intronic regions, of LU were amplified by polymerase-chain reaction, and the products were sequenced...
2012: Immunohematology
Flavia Roche Moreira Latini, André Uchimura Bastos, Carine Prisco Arnoni, Janaína Guilhem Muniz, Rosangela Medeiros Person, Wilson Baleotti, José Augusto Barreto, Lilian Castilho, Janete Maria Cerutti
Duffy or DARC (Duffy Antigen Receptor for Chemokines) is a glycosylated membrane protein that selectively binds angiogenic chemokines. Previous in vivo and in vitro studies of DARC function in cancer have associated DARC over expression with better prognosis, decreased metastatic potential, and inhibition of tumor-associated neovascularization. Another carcinogenesis-associated antigen is Lutheran or BCAM (basal cell adhesion molecule), a surface glycoprotein that acts as a receptor for the extracellular matrix protein, laminin...
March 2013: Blood Cells, Molecules & Diseases
Maria De Grandis, Marie Cambot, Marie-Paule Wautier, Bruno Cassinat, Christine Chomienne, Yves Colin, Jean-Luc Wautier, Caroline Le Van Kim, Wassim El Nemer
Polycythemia vera (PV) is characterized by an increased RBC mass, spontaneous erythroid colony formation, and the JAK2V617F mutation. PV is associated with a high risk of mesenteric and cerebral thrombosis. PV RBC adhesion to endothelial laminin is increased and mediated by phosphorylated erythroid Lu/BCAM. In the present work, we investigated the mechanism responsible for Lu/BCAM phosphorylation in the presence of JAK2V617F using HEL and BaF3 cell lines as well as RBCs from patients with PV. High levels of Rap1-GTP were found in HEL and BaF3 cells expressing JAK2V617F compared with BaF3 cells with wild-type JAK2...
January 24, 2013: Blood
Virginie Helias, Carole Saison, Thierry Peyrard, Eliane Vera, Claude Prehu, Jean-Pierre Cartron, Lionel Arnaud
KLF1 encodes an erythroid transcription factor, whose essential function in erythropoiesis has been demonstrated by extensive studies in mouse models. The first reported mutations in human KLF1 were found in individuals with a rare and asymptomatic blood type called In(Lu). Here, we show that KLF1 haploinsufficiency is responsible for the In(Lu) blood type, after redefining this peculiar blood type using flow cytometry to quantify the levels of BCAM and CD44 on red blood cells. We found 10 (seven novel) heterozygous KLF1 mutations responsible for the In(Lu) blood type...
January 2013: Human Mutation
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