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Diego blood group system

Tony L R Silveira, William B Domingues, Mariana H Remião, Lucas Santos, Bruna Barreto, Ingrid M Lessa, Antonio Sergio Varela Junior, Diego Martins Pires, Carine Corcini, Tiago Collares, Fabiana K Seixas, Ricardo B Robaldo, Vinicius F Campos
Some mammalian reference genes, which are widely used to normalize the qRT-PCR, could not be used for this purpose due to its high expression variation. The normalization with false reference genes leads to misinterpretation of results. The silversides ( Odontesthes spp.) has been used as models for evolutionary, osmoregulatory and environmental pollution studies but, up to now, there are no studies about reference genes in any Odontesthes species. Furthermore, many studies on silversides have used reference genes without previous validations...
2018: Frontiers in Genetics
Taciani de Almeida Magalhães, Diego Correia, Luana Martins de Carvalho, Samara Damasceno, Ana Lúcia Brunialti Godard
Introduction: Maternal separation is an early life stress event associated with behavioral alterations and ethanol consumption. We aimed to expand the current understanding on the molecular mechanisms mediating the impact of postnatal stress on ethanol consumption. Methods: In the first experiment (T1), some of the pups were separated from their mothers for 6 hr daily (Maternal Separation group - MS), whereas the other pups remained in the cage with their respective mothers (Control group - C)...
January 2018: Brain and Behavior
Simonetta Picone, Alberto Ritieni, Adele Fabiano, Giulia Graziani, Piermichele Paolillo, Giovanni Livolti, Fabio Galvano, Diego Gazzolo
BACKGROUND: Lutein (LT) is a naturally occurring xanthophyll carotenoid most predominant in the central nervous system (CNS), but its neurotrophic role is still debated. We therefore investigated whether cord blood concentrations correlated with a well-established neurobiomarker, namely activin A. METHODS: We conducted a prospective study on the distribution of LT and activin A in arterial cord blood of healthy preterm (n=50) and term (n=82) newborns according to weeks of gestational age (wGA) and gender...
February 2018: Clinical Biochemistry
Priscila M Ferri, Ana C Simões E Silva, Karen C L Torres, Soraya L C Silva, Diego J Q Aquino, Maria L M Ferreira, Eleonora D T Fagundes, Débora M Miranda, Alexandre R Ferreira
OBJECTIVE: The pathophysiology of autoimmune hepatitis (AIH) may involve the activation of immune cells and changes in the expression of cellular markers. The aim of the present study was to characterize the immunophenotype markers of lymphocytes and monocytes in the peripheral blood of children and adolescents with type 1 AIH and AIH overlap with sclerosing cholangitis (overlap syndrome [OS]). METHODS: This is a cross-sectional study of 20 children and adolescents diagnosed with type 1 AIH and 19 with OS...
February 2018: Journal of Pediatric Gastroenterology and Nutrition
Diego Cecchin, Alessandro Palombit, Marco Castellaro, Erica Silvestri, Franco Bui, Henryk Barthel, Osama Sabri, Maurizio Corbetta, Alessandra Bertoldo
INTRODUCTION: In the last 20 years growing attention has been devoted to multimodal imaging. The recent literature is rich of clinical and research studies that have been performed using different imaging modalities on both separate and integrated Positron Emission Tomography (PET) and Magnetic Resonance (MR) scanners. However, today, hybrid PET/MR systems measure signals related to brain structure, metabolism, neurochemistry, perfusion, and neuronal activity simultaneously, i.e. in the same physiological conditions...
July 27, 2017: Quarterly Journal of Nuclear Medicine and Molecular Imaging
Diego S T Martinez, João Paulo V Damasceno, Lidiane S Franqui, Jefferson Bettini, Italo O Mazali, Mathias Strauss
Functional mesoporous materials have been worldwide studied for different applications. Mesoporous silicas are highlighted due to the synthetic possibilities for the preparation of such materials with different particle sizes and morphologies, and controlled pores sizes and structures. Moreover, the silica superficial silanol groups are explored in several chemical modifications, leading to functional materials with tuned functionalities and properties. In this work, an organo-functionalization and pyrolysis synthetic procedure is used to obtain graphitic carbon modified mesoporous SBA-15 silica...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Daniel Cañas, Emilio A Herrera, Claudio García-Herrera, Diego Celentano, Bernardo J Krause
Aim: Fetal growth restriction (FGR) is associated with a variety of cardiometabolic diseases in adulthood which could involve remodeling processes of the vascular walls that could start in the fetal period. However, there is no consensus whether this remodeling affects in a similar way the whole vascular system. We aimed to determine the effects of FGR on the vasoactive and biomechanical properties of umbilical and systemic vessels in fetal guinea pigs. Methods: FGR was induced by implanting ameroid occluders at mid-gestation in uterine arteries of pregnant guinea pigs, whilst the control group was exposed to simulated surgery...
2017: Frontiers in Physiology
Felipe Terres de Campos, Joao Aveiro Alvarado Rincon, Diego Andres Velasco Acosta, Pedro Augusto Silva Silveira, Jorgea Pradieé, Marcio Nunes Corrêa, Bernardo Garziera Gasperin, Luiz Francisco Machado Pfeifer, Carlos Castilho Barros, Ligia Margareth Cantareli Pegoraro, Augusto Schneider
The aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) - estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal...
February 2017: Theriogenology
Daniel Dansdill, Pegge M Halandras, Joshua Beverly, Walter Jeske, Debra Hoppensteadt, Martin Emanuele, Jawed Fareed, Jae S Cho
OBJECTIVE: Poloxamer-188 is a synthetic, organic compound that acts by binding hydrophobic pockets on damaged lipid bilayers in the circulation. P-188 reduces blood viscosity and confers anti-inflammatory and cytoprotective effects. Vepoloxamer (Mast Therapeutics, San Diego, Calif) is a purified version of this compound that has limited side effects. The aim of this study was to investigate drug interactions between vepoloxamer and heparin and tissue plasminogen activator (tPA). METHODS: An experimental rat tail transection model was used to study vepoloxamer's interaction with heparin...
January 2018: Journal of Vascular Surgery
Yanli Ji, Jizhi Wen, Barbera Veldhuisen, Lonneke Haer-Wigman, Zhen Wang, Martin Lodén-van Straaten, Ling Wei, Guangping Luo, Yongshui Fu, C Ellen van der Schoot
BACKGROUND: Genotyping platforms for common red blood cell (RBC) antigens have been successfully applied in Caucasian and black populations but not in Chinese populations. In this study, a genotyping assay based on multiplex ligation-dependent probe amplification (MLPA) technology was applied in a Chinese population to validate the MLPA probes. Subsequently, the comprehensive distribution of 17 blood group systems also was obtained. STUDY DESIGN AND METHODS: DNA samples from 200 Chinese donors were extracted and genotyped using the blood-MLPA assay...
February 2017: Transfusion
Oytip Nathalang, Puangpaka Panichrum, Kamphon Intharanut, Phatchira Thattanon, Siriporn Nathalang
Alloantibodies to the Diego (DI) blood group system, anti-Dia and anti-Dib are clinically significant in causing hemolytic transfusion reactions (HTRs) and hemolytic disease of the fetus and newborn (HDFN), especially in Asian populations with Mongolian ancestry. This study aimed to report the frequency of the DI*A and DI*B alleles in a Central Thai population and to compare them with those of other populations previously published. Altogether, 1,011 blood samples from unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok were included...
2016: PloS One
Krzysztof Mikołajczyk, Radosław Kaczmarek, Marcin Czerwiński
Transcription factor EKLF (Erythroid Krüppel-Like Factor) belongs to the group of Krüppellike factors, which regulate proliferation, differentiation, development and apoptosis of mammalian cells. EKLF factor is present in erythroid cells, where it participates in regulation of hematopoiesis, expression of genes encoding transmembrane proteins (including blood group antigens), and heme biosynthesis enzymes. It is also a key factor in downregulation of γ-globins and activation of β-globin gene expression...
October 6, 2016: Postȩpy Higieny i Medycyny Doświadczalnej
Joana Maira Valentini Zacarias, Ieda Bernadete Volkweis Langer, Jeane Eliete Laguila Visentainer, Ana Maria Sell
The aim of this study was to assess the distribution of alleles and genotypes of the blood group systems Rh, Kell, Duffy, Kidd, and Diego in 251 regular blood donors registered in the hemotherapy unit of the Southwestern region of Paraná, Southern Brazil. The frequencies were obtained by direct counting on a spreadsheet program and statistical analyses were conducted in order to compare them with other Brazilian populations using chi-squared with Yates correction on OpenEpi software. The frequencies of RHD* negative, RHCE*c/c and RHCE*e/e were higher than expected for the Caucasian population...
December 2016: Transfusion and Apheresis Science
Daiane Cobianchi Costa, Alessandra Arruda Schinaider, Thais Mattos Santos, Everaldo José Schörner, Daniel Simon, Sharbel Weidner Maluf, Ana Carolina Rabello de Moraes, Maria Claudia Silva Silva
BACKGROUND: Red blood cell genes are highly polymorphic with the distribution of alleles varying between different populations and ethnic groups. The objective of this study was to investigate gene polymorphisms of blood groups in the state of Santa Catarina, Southern Brazil. METHODS: Three hundred and seventy-three unrelated blood donors and 31 transfusion-dependent patients were evaluated to investigate polymorphisms of the Rh, Kell, Duffy, Kidd, and Diego blood group systems in a population from the state of Santa Catarina...
July 2016: Revista Brasileira de Hematologia e Hemoterapia
Claudia Mora, Jorge Medina-Rosas, Ana Maria Santos, Diego A Jaimes, Ana M Arbeláez, Consuelo Romero, Annie Cortes, John Londono
OBJECTIVE: There are no laboratory tools that detect early flares in systemic lupus erythematosus (SLE). Our aim was to validate in our population the previous findings of the association of C4d-bearing reticulocytes (R-C4d) compared to anti-dsDNA antibodies, with disease activity assessed by the Safety of Estrogens in Lupus Erythematosus National Assessment-Systemic Lupus Erythematosus Disease Activity Index (SELENA-SLEDAI) and the British Isles Lupus Assessment Group (BILAG) 2004 scales...
September 2016: Journal of Rheumatology
Paula Vélez, Raymundo Ocaranza-Sánchez, Diego López-Otero, Lilian Grigorian-Shamagian, Isaac Rosa, Susana Belén Bravo, José Ramón González-Juanatey, Ángel García
PURPOSE: Platelets play a fundamental role in the atherothrombotic events that lead to an acute myocardial infarction. In the present study we compared the proteome of intracoronary and peripheral arterial platelets from ST-elevation myocardial infarction (STEMI) patients in the search for potential platelet biomarkers/drug targets related to what is happening at the culprit site. EXPERIMENTAL DESIGN: Ten STEMI patients were recruited and blood collected from the occluded coronary artery, at the culprit site, in the moment of reperfusion...
August 2016: Proteomics. Clinical Applications
Mara Boschetti, Sergio Agosti, Valeria Albanese, Laura Casalino, Claudia Teti, Gian Paolo Bezante, Claudio Brunelli, Manuela Albertelli, Diego Ferone
Hypopituitarism reduces life expectancy and increases the risk of cardiovascular and cerebrovascular diseases, as well as death. Abnormalities in the cardiovascular system may be independently related to GH deficiency (GHD). The aim of this study was to prospectively investigate coronary flow reserve and diastolic function in GHD adult patients at diagnosis and after 1 year of GH replacement therapy. As control group, an age- and sex-matched population was chosen. All patients and controls were non-smokers, non-diabetic, and normotensive, with no history of vascular disease...
February 2017: Endocrine
José María Pérez-Pomares, José Luis de la Pompa, Diego Franco, Deborah Henderson, Siew Yen Ho, Lucile Houyel, Robert G Kelly, David Sedmera, Mary Sheppard, Silke Sperling, Gaetano Thiene, Maurice van den Hoff, Cristina Basso
Congenital coronary artery anomalies are of major significance in clinical cardiology and cardiac surgery due to their association with myocardial ischaemia and sudden death. Such anomalies are detectable by imaging modalities and, according to various definitions, their prevalence ranges from 0.21 to 5.79%. This consensus document from the Development, Anatomy and Pathology Working Group of the European Society of Cardiology aims to provide: (i) a definition of normality that refers to essential anatomical and embryological features of coronary vessels, based on the integrated analysis of studies of normal and abnormal coronary embryogenesis and pathophysiology; (ii) an animal model-based systematic survey of the molecular and cellular mechanisms that regulate coronary blood vessel development; (iii) an organization of the wide spectrum of coronary artery anomalies, according to a comprehensive anatomical and embryological classification scheme; (iv) current knowledge of the pathophysiological mechanisms underlying symptoms and signs of coronary artery anomalies, with diagnostic and therapeutic implications...
February 1, 2016: Cardiovascular Research
Chunxia Chen, Jinzhe Tan, Lixin Wang, Bing Han, Wei Sun, Li Zhao, Chunyan Huang, Bin Tan, Li Qin
BACKGROUND: Unexpected antibodies are frequently associated with hemolytic disease of the fetus and newborn (HDFN) and hemolytic transfusion reactions (HTRs), and screening for those unexpected antibodies is critical for the safety and effectiveness of transfusion. Different populations differ in the prevalence of significant antibodies and also the low-frequency red blood cell (RBC) antigens. In China, antibody screening has been a common practice for pretransfusion testing for more than 10 years...
April 2016: Transfusion
Salvatore Torre, Elisa Biondani, Tiziano Menon, Diego Marchi, Mauro Franzoi, Daniele Ferrarini, Rocco Tabbì, Stiljan Hoxha, Luca Barozzi, Giuseppe Faggian, Giovanni Battista Luciani
Cardiopulmonary bypass (CPB) in infants is associated with morbidity due to systemic inflammatory response syndrome (SIRS). Strategies to mitigate SIRS include management of perfusion temperature, hemodilution, circuit miniaturization, and biocompatibility. Traditionally, perfusion parameters have been based on body weight. However, intraoperative monitoring of systemic and cerebral metabolic parameters suggest that often, nominal CPB flows may be overestimated. The aim of the study was to assess the safety and efficacy of continuous metabolic monitoring to manage CPB in infants during open-heart repair...
January 2016: Artificial Organs
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