Read by QxMD icon Read

Colon cancer exosomes

Takeshi Harada, Hideki Yamamoto, Shosei Kishida, Michiko Kishida, Chihiro Awada, Toshifumi Takao, Akira Kikuchi
Wnt5b, a member of the same family of proteins as Wnt5a of which overexpression is associated with cancer aggressiveness, is suggested to be involved in cancer progression, however details remain unclarified. We analyzed biochemical properties of purified Wnt5b and the mode of secretion of Wnt5b by cancer cells. Wnt5b was glycosylated at three asparagine residues and lipidated at one serine residue, and these post-translational modifications of Wnt5b are essential for secretion. Purified Wnt5b showed Dvl2 phosphorylation and Rac activation abilities to a similar extent as Wnt5a...
October 20, 2016: Cancer Science
Shu Wen Wen, Jaclyn Sceneay, Luize G Lima, Christina Sf Wong, Melanie Becker, Sophie Krumeich, Richard J Lobb, Vanessa Castillo, Ke Ni Wong, Sarah Ellis, Belinda S Parker, Andreas Moller
Small membranous secretions from tumor cells, termed exosomes, contribute significantly to intercellular communication and subsequent reprogramming of the tumor microenvironment. Here we use optical imaging to determine that exogenously administered fluorescently-labeled exosomes derived from highly metastatic murine breast cancer cells, distributed predominantly to the lung of syngeneic mice, a frequent site of breast cancer metastasis. At the sites of accumulation, exosomes were taken up by CD45+ bone marrow-derived cells...
October 19, 2016: Cancer Research
Diego De Miguel, Ana Gallego-Lleyda, José María Ayuso, Dolores Pejenaute-Ochoa, Vidal Jarauta, Isabel Marzo, Luis J Fernández, Ignacio Ochoa, Blanca Conde, Alberto Anel, Luis Martinez-Lostao
During the last years, a great effort has been invested into developing new TRAIL formulations with increased bioactivity, trying to overcome the resistance to conventional soluble TRAIL (sTRAIL) exhibited by many primary tumours. In our group, we have generated artificial lipid nanoparticles decorated with sTRAIL (LUV-TRAIL), emulating the physiological TRAIL-containing exosomes by which T-cells release TRAIL upon activation. We already demonstrated that LUV-TRAIL has greater cytotoxicity against both chemoresistant haematologic tumour cells and epithelial carcinoma cells compared to a form of sTRAIL similar to that used in clinical trials...
October 8, 2016: Cancer Letters
Elisabetta Bigagli, Cristina Luceri, Daniele Guasti, Lorenzo Cinci
Cancer-secreted exosomes influence tumor microenvironment and support cancer growth and metastasis. MiR-210 is frequently up-regulated in colorectal cancer tissues and correlates with metastatic disease. We investigated whether exosomes are actively released by HCT-8 colon cancer cells, the role of exosomal miR-210 in the cross-talk between primary cancer cells and neighboring metastatic cells and its contribution in regulating epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET)...
August 11, 2016: Cancer Biology & Therapy
Zongwei Li, Ming Zhuang, Lichao Zhang, Xingnan Zheng, Peng Yang, Zhuoyu Li
High glucose-regulated protein 78 (GRP78) expression contributes to the acquisition of a wide range of phenotypic cancer hallmarks, and the pleiotropic oncogenic functions of GRP78 may result from its diverse subcellular distribution. Interestingly, GRP78 has been reported to be secreted from solid tumour cells, participating in cell-cell communication in the tumour microenvironment. However, the mechanism underlying this secretion remains elusive. Here, we report that GRP78 is secreted from colon cancer cells via exosomes...
2016: Scientific Reports
Luana Lugini, Mauro Valtieri, Cristina Federici, Serena Cecchetti, Stefania Meschini, Maria Condello, Michele Signore, Stefano Fais
BACKGROUND: Cancer cells, including colorectal cancer ones (CRC), release high amounts of nanovesicles (exosomes), delivering biochemical messages for paracrine or systemic crosstalk. Mesenchymal stromal cells (MSCs) have been shown to play contradicting roles in tumor progression. RESULTS: CRC exosomes induce in cMSCs: i) atypical morphology, higher proliferation, migration and invasion; ii) formation of spheroids; iii) an acidic extracellular environment associated with iv) a plasma membrane redistribution of vacuolar H+-ATPase and increased expression of CEA...
July 13, 2016: Oncotarget
Andrew J McKenzie, Daisuke Hoshino, Nan Hyung Hong, Diana J Cha, Jeffrey L Franklin, Robert J Coffey, James G Patton, Alissa M Weaver
Secretion of RNAs in extracellular vesicles is a newly recognized form of intercellular communication. A potential regulatory protein for microRNA (miRNA) secretion is the critical RNA-induced silencing complex (RISC) component Argonaute 2 (Ago2). Here, we use isogenic colon cancer cell lines to show that overactivity of KRAS due to mutation inhibits localization of Ago2 to multivesicular endosomes (MVEs) and decreases Ago2 secretion in exosomes. Mechanistically, inhibition of mitogen-activated protein kinase kinases (MEKs) I and II, but not Akt, reverses the effect of the activating KRAS mutation and leads to increased Ago2-MVE association and increased exosomal secretion of Ago2...
May 3, 2016: Cell Reports
Allison L Isola, Suzie Chen
The activation of G-Protein Coupled Receptors (GPCRs) by their respective ligands initiates a cascade of multiple signaling processes within the cell, regulating growth, metabolism and other essential cellular functions. Dysregulation and aberrant expression of these GPCRs and their subsequent signaling cascades are associated with many different types of pathologies, including cancer. The main life threatening complication in patients diagnosed with cancer is the dissemination of cells from the primary tumor to distant vital organs within the body, metastasis...
2016: Frontiers in Genetics
Dominique Thuringer, Gaetan Jego, Kevin Berthenet, Arlette Hammann, Eric Solary, Carmen Garrido
Gap junctional communication between cancer cells and blood capillary cells is crucial to tumor growth and invasion. Gap junctions may transfer microRNAs (miRs) among cells. Here, we explore the impact of such a transfer in co-culture assays, using the antitumor miR-145 as an example. The SW480 colon carcinoma cells form functional gap junction composed of connexin-43 (Cx43) with human microvascular endothelial cells (HMEC). When HMEC are loaded with miR-145-5p mimics, the miR-145 level drastically increases in SW480...
May 10, 2016: Oncotarget
Ulrich H Weidle, Fabian Birzele, Gwendlyn Kollmorgen, Rüdiger Rüger
A predilection of metastasis to the lungs has been noted for several types of cancer. Herein, we summarize underlying mechanisms for lung tropism of metastasis. We discuss the identification of a gene signature in primary breast tumors predicting metastasis to the lungs, as well as functional validation of selected genes of the signature. We outline the contribution of pre- and metastatic niches, the role of exosomes, activation of disseminated, dormant tumor cells and selected tumor-stromal cell interactions to lung metastasis and colonization...
March 2016: Cancer Genomics & Proteomics
Florin Zaharie, Mihai Stefan Muresan, Bobe Petrushev, Cristian Berce, Grigore-Aristide Gafencu, Sonia Selicean, Ancuta Jurj, Roxana Cojocneanu-Petric, Cosmin-Ioan Lisencu, Laura-Ancuta Pop, Valentina Pileczki, Dan Eniu, Mihai-Andrei Muresan, Roxana Zaharie, Ioana Berindan-Neagoe, Ciprian Tomuleasa, Alexandru Irimie
BACKGROUND AND AIMS: Worldwide, colorectal cancer (CRC) is the third most common cancer in men and second in women. The aim of the current study was to identify whether the miR-375 is indeed down-regulated in metastatic CRC and if it could be considered as a potential minimally invasive prognostic biomarker for CRC. METHODS: Exosomes were isolated and characterized from patients with liver metastasis from CCR. The characterization of exosome was performed using TEM/SEM...
December 2015: Journal of Gastrointestinal and Liver Diseases: JGLD
Jessica Gobbo, Guillaume Marcion, Marine Cordonnier, Alexandre M M Dias, Nicolas Pernet, Arlette Hammann, Sarah Richaud, Hajare Mjahed, Nicolas Isambert, Victor Clausse, Cédric Rébé, Aurélie Bertaut, Vincent Goussot, Frédéric Lirussi, François Ghiringhelli, Aurélie de Thonel, Pierre Fumoleau, Renaud Seigneuric, Carmen Garrido
BACKGROUND: Exosomes, via heat shock protein 70 (HSP70) expressed in their membrane, are able to interact with the toll-like receptor 2 (TLR2) on myeloid-derived suppressive cells (MDSCs), thereby activating them. METHODS: We analyzed exosomes from mouse (C57Bl/6) and breast, lung, and ovarian cancer patient samples and cultured cancer cells with different approaches, including nanoparticle tracking analysis, biolayer interferometry, FACS, and electron microscopy...
March 2016: Journal of the National Cancer Institute
Jacqueline Banyard, Diane R Bielenberg
Metastatic cancer cells are lethal. Understanding the molecular mechanisms that bolster the conversion from benign to malignant progression is key for treating these heterogeneous and resistant neoplasms. The epithelial-mesenchymal transition (EMT) is a conserved cellular program that alters cell shape, adhesion and movement. The shift to a more mesenchymal-like phenotype can promote tumor cell intravasation of surrounding blood vessels and emigration to a new organ, yet may not be necessary for extravasation or colonization into that environment...
2015: Connective Tissue Research
Claudia Campanella, Francesca Rappa, Carmelo Sciumè, Antonella Marino Gammazza, Rosario Barone, Fabio Bucchieri, Sabrina David, Giuseppe Curcurù, Celeste Caruso Bavisotto, Alessandro Pitruzzella, Girolamo Geraci, Giuseppe Modica, Felicia Farina, Giovanni Zummo, Stefano Fais, Everly Conway de Macario, Alberto J L Macario, Francesco Cappello
BACKGROUND: Heat shock protein 60 (Hsp60) is a chaperonin involved in tumorigenesis, but its participation in tumor development and progression is not well understood and its value as a tumor biomarker has not been fully elucidated. In the current study, the authors presented evidence supporting the theory that Hsp60 has potential as a biomarker as well as a therapeutic target in patients with large bowel cancer. METHODS: The authors studied a population of 97 subjects, including patients and controls...
September 15, 2015: Cancer
Jun Zhou, Guanghui Gong, Hong Tan, Furong Dai, Xin Zhu, Yile Chen, Junpu Wang, Ying Liu, Puxiang Chen, Xiaoying Wu, Jifang Wen
MicroRNAs (miRNAs) can serve as biomarkers in human cancer. To determine the clinical value of urinary miRNAs for ovarian serous adenocarcinoma, we collected urine samples from 39 ovarian serous adenocarcinoma patients, 26 patients with benign gynecological disease and 30 healthy controls. The miRNA microarray data showed that only miR-30a-5p was upregulated and 37 miRNAs were downregulated in the urine samples of ovarian serous adenocarcinoma patients, when compared to healthy controls, which was confirmed after conducting quantitative PCR...
June 2015: Oncology Reports
Zhongbin Deng, Jingyao Mu, Michael Tseng, Binks Wattenberg, Xiaoying Zhuang, Nejat K Egilmez, Qilong Wang, Lifeng Zhang, James Norris, Haixun Guo, Jun Yan, Bodduluri Haribabu, Donald Miller, Huang-Ge Zhang
Gut-associated inflammation plays a crucial role in the progression of colon cancer. Here, we identify a novel pathogen-host interaction that promotes gut inflammation and the development of colon cancer. We find that enteropathogenic bacteria-secreted particles (ET-BSPs) stimulate intestinal epithelium to produce IDENs (intestinal mucosa-derived exosome-like nanoparticles) containing elevated levels of sphingosine-1-phosphate, CCL20 and prostaglandin E2 (PGE2). CCL20 and PGE2 are required for the recruitment and proliferation, respectively, of Th17 cells, and these processes also involve the MyD88-mediated pathway...
2015: Nature Communications
Rong Xu, David W Greening, Alin Rai, Hong Ji, Richard J Simpson
Secretion and exchange of extracellular vesicles (EVs) by most cell types is emerging as a fundamental biological process. Although much is known about EVs, there is still a lack of definition as to how many naturally occurring EV subtypes there are and how their properties and functionalities might differ. This vexing issue is critical if EVs are to be fully harnessed for therapeutic applications. To address this question we have developed and describe here a sequential centrifugal ultrafiltration (SCUF) method to examine, in an unbiased manner, what EV subtypes are released in vitro into cell culture medium using the human colon carcinoma cell line LIM1863 as a model system...
October 1, 2015: Methods: a Companion to Methods in Enzymology
I G Nikitina, E Iu Sabirova, O N Solopova, S A Surzhikov, E N Grineva, V L Karpov, N A Lisitsyn, S F Beresten'
Anew immuno-PCR format is described that is based on detection of membrane protein CDH17 in serum exosomes. Format application allows distinction between sera samples of healthy donors and colon cancer patients. Obtained results open a possibility of serological colon cancer diagnosis in high risk groups.
January 2014: Molekuliarnaia Biologiia
Daniel Senfter, Silvio Holzner, Maria Kalipciyan, Anna Staribacher, Angelika Walzl, Nicole Huttary, Sigurd Krieger, Stefan Brenner, Walter Jäger, Georg Krupitza, Helmut Dolznig, Robert M Mader
Invasive colorectal cancer is associated with poor prognosis requiring treatment with systemic chemotherapies usually including 5-fluorouracil. A consequence of prolonged treatment is the acquisition of resistance eventually resulting in the recurrence of highly metastatic cancer cells. To address the relationship between drug resistance and increased lymphatic metastatic potential, we used a 3D co-culture model of colon tumour cell spheroids of parent CCL227 cells and subclones with gradually increasing resistance against 5-fluorouracil...
July 1, 2015: Human Molecular Genetics
Garth L Nicolson
Cancer cells are surrounded by a fluid-mosaic membrane that provides a highly dynamic structural barrier with the microenvironment, communication filter and transport, receptor and enzyme platform. This structure forms because of the physical properties of its constituents, which can move laterally and selectively within the membrane plane and associate with similar or different constituents, forming specific, functional domains. Over the years, data have accumulated on the amounts, structures, and mobilities of membrane constituents after transformation and during progression and metastasis...
April 1, 2015: Cancer Research
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"