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https://www.readbyqxmd.com/read/27923683/role-of-a-tachykinin-related-peptide-and-its-receptor-in-modulating-the-olfactory-sensitivity-in-the-oriental-fruit-fly-bactrocera-dorsalis-hendel
#1
Shun-Hua Gui, Hong-Bo Jiang, Li Xu, Yu-Xia Pei, Xiao-Qiang Liu, Guy Smagghe, Jin-Jun Wang
Insect tachykinin-related peptide (TRP), an ortholog of tachykinin in vertebrates, has been linked with regulation of diverse physiological processes, such as olfactory perception, locomotion, aggression, lipid metabolism and myotropic activity. In this study, we investigated the function of TRP (BdTRP) and its receptor (BdTRPR) in an important agricultural pest, the oriental fruit fly Bactrocera dorsalis. BdTRPR is a typical G-protein coupled-receptor (GPCR), and it could be activated by the putative BdTRP mature peptides with the effective concentrations (EC50) at the nanomolar range when expressed in Chinese hamster ovary cells...
December 3, 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27918112/validation-of-adam10-metalloprotease-as-a-bacillus-thuringiensis-cry3aa-toxin-functional-receptor-in-colorado-potato-beetle-leptinotarsa-decemlineata
#2
V M Ruiz-Arroyo, I García-Robles, C Ochoa-Campuzano, G A Goig, E Zaitseva, G Baaken, A C Martínez-Ramírez, C Rausell, M D Real
Bacillus thuringiensis parasporal crystal proteins (Cry proteins) are insecticidal pore-forming toxins that bind to specific receptor molecules on the brush border membrane of susceptible insect midgut cells to exert their toxic action. In the Colorado potato beetle (CPB), a coleopteran pest, we previously proposed that interaction of Cry3Aa toxin with a CPB ADAM10 metalloprotease is an essential part of the mode of action of this toxin. Here, we annotated the gene sequence encoding an ADAM10 metalloprotease protein (CPB-ADAM10) in the CPB genome sequencing project, and using RNA interference gene silencing we demonstrated that CPB-ADAM10 is a Cry3Aa toxin functional receptor in CPB...
December 5, 2016: Insect Molecular Biology
https://www.readbyqxmd.com/read/27914919/expressing-a-moth-abcc2-gene-in-transgenic-drosophila-causes-susceptibility-to-bt-cry1ac-without-requiring-a-cadherin-like-protein-receptor
#3
Tristan Stevens, Sisi Song, John B Bruning, Amanda Choo, Simon W Baxter
Bt toxins ingested by insect pests can bind to midgut receptors and cause death, although several steps in this process remain unclear. Multiple Bt toxin receptors have been identified in Lepidoptera, including a cadherin-like protein (CaLP), which is central to several models explaining Bt toxins' mode of action. Mutations in the Plutella xylostella ATP-dependent binding cassette transporter C2 (Px-abcc2), rather than CaLP, are genetically linked with Bt Cry1Ac resistance. Here we expressed Px-abcc2 in Drosophila and performed larval bioassays to determine whether this protein acts as an effective Bt receptor...
November 30, 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27908826/itraq-based-quantitative-proteomic-analysis-of-midgut-in-silkworm-infected-with-bombyx-mori-cytoplasmic-polyhedrosis-virus
#4
Kun Gao, Xiang-Yuan Deng, Meng-Ke Shang, Guang-Xing Qin, Cheng-Xiang Hou, Xi-Jie Guo
: Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) specifically infects the epithelial cells in the midgut of silkworm and causes them to death, which negatively affects the sericulture industry. In order to determine the midgut response at the protein levels to the virus infection, differential proteomes of the silkworm midgut responsive to BmCPV infection were identified with isobaric tags for relative and absolute quantitation (iTRAQ) labeling followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
November 28, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27867074/toxicity-of-cry1a-toxins-from-bacillus-thuringiensis-to-cf1-cells-does-not-involve-activation-of-adenylate-cyclase-pka-signaling-pathway
#5
Leivi Portugal, Carlos Muñóz-Garay, Diana L Martínez de Castro, Mario Soberón, Alejandra Bravo
Bacillus thuringiensis (Bt) bacteria produce Cry toxins that are able to kill insect pests. Different models explaining the mode of action of these toxins have been proposed. The pore formation model proposes that the toxin creates pores in the membrane of the larval midgut cells after interaction with different receptors such as cadherin, aminopeptidase N and alkaline phosphatase and that this pore formation activity is responsible for the toxicity of these proteins. The alternative model proposes that interaction with cadherin receptor triggers an intracellular cascade response involving protein G, adenylate cyclase (AC) and protein kinase A (PKA)...
November 17, 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27823898/rnai-in-the-striped-stem-borer-chilo-suppressalis-establishes-a-functional-role-for-aminopeptidase-n-in-cry1ab-intoxication
#6
X Y Wang, L X Du, C X Liu, L Gong, L Z Han, Y F Peng
The striped stem borer, Chilo suppressalis, is a major target pest of transgenic rice expressing the Cry1Ab protein from the bacterium Bacillus thuringiensis (Bt) in China. Evolution of resistance in this pest is a major threat to the durability of Bt rice. Since Bt exerts its activity through binding to specific receptors in the midgut of target insects, identification of functional Cry1Ab receptors in the midgut of C. suppressalis larvae is crucial to evaluate potential resistance mechanisms and develop effective strategies for delaying insect resistance...
November 4, 2016: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/27685262/specific-endocytosis-blockade-of-trypanosoma-cruzi-exposed-to-a-poly-lacnac-binding-lectin-suggests-that-lectin-sugar-interactions-participate-to-receptor-mediated-endocytosis
#7
Sébastien Brosson, Frédéric Fontaine, Marjorie Vermeersch, David Perez-Morga, Etienne Pays, Sabrina Bousbata, Didier Salmon
Trypanosoma cruzi is a protozoan parasite transmitted by a triatomine insect, and causing human Chagas disease in South America. This parasite undergoes a complex life cycle alternating between non-proliferative and dividing forms. Owing to their high energy requirement, replicative epimastigotes of the insect midgut display high endocytic activity. This activity is mainly restricted to the cytostome, by which the cargo is taken up and sorted through the endosomal vesicular network to be delivered to reservosomes, the final lysosomal-like compartments...
2016: PloS One
https://www.readbyqxmd.com/read/27677695/characterization-of-a-neuropeptide-f-receptor-in-the-tsetse-fly-glossina-morsitans-morsitans
#8
Jelle Caers, Matthias B Van Hiel, Katleen Peymen, Sven Zels, Liesbeth Van Rompay, Jan Van Den Abbeele, Liliane Schoofs, Isabel Beets
Neuropeptides related to mammalian neuropeptide Y (NPY) and insect neuropeptide F (NPF) are conserved throughout Metazoa and intimately involved in a wide range of biological processes. In insects NPF is involved in regulating feeding, learning, stress and reproductive behavior. Here we identified and characterized an NPF receptor of the tsetse fly, Glossina morsitans morsitans, the sole transmitter of Trypanosoma parasites causing sleeping sickness. We isolated cDNA sequences encoding tsetse NPF (Glomo-NPF) and its receptor (Glomo-NPFR), and examined their spatial and temporal expression patterns using quantitative PCR...
September 24, 2016: Journal of Insect Physiology
https://www.readbyqxmd.com/read/27620558/developmental-expression-of-ecdysone-related-genes-associated-with-metamorphic-changes-during-midgut-remodeling-of-silkworm-bombyx-mori-lepidoptera-bombycidae
#9
Ebru Goncu, Ramazan Uranlı, Gozde Selek, Osman Parlak
Steroid hormone 20-hydroxyecdysone is known as the systemic regulators of insect cells; however, how to impact the fate and function of mature and stem cells is unclear. For the first time, we report ecdysone regulatory cascades in both mature midgut cell and stem cell fractions related to developmental events by using histological, immunohistochemical, biochemical and gene expression analysis methods. Ecdysone receptor-B1 (EcR-B1) and ultraspiracle 1 (USP-1) mRNAs were detected mainly in mature cells during programmed cell death (PCD)...
2016: Journal of Insect Science
https://www.readbyqxmd.com/read/27336743/de-novo-transcriptome-analysis-of-the-common-new-zealand-stick-insect-clitarchus-hookeri-phasmatodea-reveals-genes-involved-in-olfaction-digestion-and-sexual-reproduction
#10
Chen Wu, Ross N Crowhurst, Alice B Dennis, Victoria G Twort, Shanlin Liu, Richard D Newcomb, Howard A Ross, Thomas R Buckley
Phasmatodea, more commonly known as stick insects, have been poorly studied at the molecular level for several key traits, such as components of the sensory system and regulators of reproduction and development, impeding a deeper understanding of their functional biology. Here, we employ de novo transcriptome analysis to identify genes with primary functions related to female odour reception, digestion, and male sexual traits in the New Zealand common stick insect Clitarchus hookeri (White). The female olfactory gene repertoire revealed ten odorant binding proteins with three recently duplicated, 12 chemosensory proteins, 16 odorant receptors, and 17 ionotropic receptors...
2016: PloS One
https://www.readbyqxmd.com/read/27174746/allatostatin-c-antagonizes-the-synergistic-myostimulatory-effect-of-allatotropin-and-serotonin-in-rhodnius-prolixus-stal
#11
María José Villalobos-Sambucaro, Luis Anibal Diambra, Fernando Gabriel Noriega, Jorge Rafael Ronderos
Haematophagous insects can ingest large quantities of blood in a single meal producing a large quantity of urine in the following hours to eliminate the excess of water and mineral ions incorporated. The excretory activity of the Malpighian tubules is facilitated by an increase in haemolymph circulation as a result of the intensification of aorta contractions, combined with an increase of anterior midgut peristaltic waves. We have recently shown that haemolymph circulation during post-prandial diuresis is modulated by the synergistic activity of allatotropin (AT) and serotonin, resulting in an increase in aorta and crop contraction rates...
July 1, 2016: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/27172217/evolution-expression-and-function-of-nonneuronal-ligand-gated-chloride-channels-in-drosophila-melanogaster
#12
Emily J Remnant, Adam Williams, Chris Lumb, Ying Ting Yang, Janice Chan, Sebastian Duchêne, Phillip J Daborn, Philip Batterham, Trent Perry
Ligand-gated chloride channels have established roles in inhibitory neurotransmission in the nervous systems of vertebrates and invertebrates. Paradoxically, expression databases in Drosophila melanogaster have revealed that three uncharacterized ligand-gated chloride channel subunits, CG7589, CG6927, and CG11340, are highly expressed in nonneuronal tissues. Furthermore, subunit copy number varies between insects, with some orders containing one ortholog, whereas other lineages exhibit copy number increases...
2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27095284/characterization-of-the-resistance-to-vip3aa-in-helicoverpa-armigera-from-australia-and-the-role-of-midgut-processing-and-receptor-binding
#13
Maissa Chakroun, Núria Banyuls, Tom Walsh, Sharon Downes, Bill James, Juan Ferré
Crops expressing genes from Bacillus thuringiensis (Bt crops) are among the most successful technologies developed for the control of pests but the evolution of resistance to them remains a challenge. Insect resistant cotton and maize expressing the Bt Vip3Aa protein were recently commercialized, though not yet in Australia. We found that, although relatively high, the frequency of alleles for resistance to Vip3Aa in field populations of H. armigera in Australia did not increase over the past four seasons until 2014/15...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27001423/mechanistic-insights-into-the-first-lygus-active-%C3%AE-pore-forming-protein
#14
Agoston Jerga, Danqi Chen, Chunfen Zhang, Jinping Fu, Jean-Louis K Kouadio, Yanfei Wang, Stephen M G Duff, Jennifer E Howard, Timothy J Rydel, Artem G Evdokimov, Parthasarathy Ramaseshadri, Adam Evans, Renata Bolognesi, Yoonseong Park, Jeffrey A Haas
The cotton pests Lygus hesperus and Lygus lineolaris can be controlled by expressing Cry51Aa2.834_16 in cotton. Insecticidal activity of pore-forming proteins is generally associated with damage to the midgut epithelium due to pores, and their biological specificity results from a set of key determinants including proteolytic activation and receptor binding. We conducted mechanistic studies to gain insight into how the first Lygus-active β-pore forming protein variant functions. Biophysical characterization revealed that the full-length Cry51Aa2...
June 15, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/26935135/bacterial-vegetative-insecticidal-proteins-vip-from-entomopathogenic-bacteria
#15
REVIEW
Maissa Chakroun, Núria Banyuls, Yolanda Bel, Baltasar Escriche, Juan Ferré
Entomopathogenic bacteria produce insecticidal proteins that accumulate in inclusion bodies or parasporal crystals (such as the Cry and Cyt proteins) as well as insecticidal proteins that are secreted into the culture medium. Among the latter are the Vip proteins, which are divided into four families according to their amino acid identity. The Vip1 and Vip2 proteins act as binary toxins and are toxic to some members of the Coleoptera and Hemiptera. The Vip1 component is thought to bind to receptors in the membrane of the insect midgut, and the Vip2 component enters the cell, where it displays its ADP-ribosyltransferase activity against actin, preventing microfilament formation...
June 2016: Microbiology and Molecular Biology Reviews: MMBR
https://www.readbyqxmd.com/read/26896568/expression-of-ryamide-in-the-nervous-and-endocrine-system-of-bombyx-mori
#16
Ladislav Roller, Daniel Čižmár, Branislav Bednár, Dušan Žitňan
RYamides are neuropeptides encoded by a gene whose precise expression and function have not yet been determined. We identified the RYamide gene transcript (fmgV1g15f, SilkBase database) and predicted two candidates for G-protein coupled RYamide receptors (A19-BAG68418 and A22-BAG68421) in the silkworm Bombyx mori. We cloned the RYamide transcript and described its spatial expression using in situ hybridisation. In the larval central nervous system (CNS) expression of RYamide was restricted to 12-14 small neurons in the brain and two posterior neurons in the terminal abdominal ganglion...
June 2016: Peptides
https://www.readbyqxmd.com/read/26847606/molecular-characterization-and-expression-profiles-of-nicotinic-acetylcholine-receptors-in-the-rice-striped-stem-borer-chilo-suppressalis-lepidoptera-crambidae
#17
Gang Xu, Shun-Fan Wu, Zi-Wen Teng, Hong-Wei Yao, Qi Fang, Jia Huang, Gong-Yin Ye
Nicotinic acetylcholine receptors (nAChRs) are members of the cys-loop ligand-gated ion channel (cysLGIC) superfamily, mediating fast synaptic cholinergic transmission in the central nervous system in insects. Insect nAChRs are the molecular targets of economically important insecticides, such as neonicotinoids and spinosad. Identification and characterization of the nAChR gene family in the rice striped stem borer, Chilo suppressalis, could provide beneficial information about this important receptor gene family and contribute to the investigation of the molecular modes of insecticide action and resistance for current and future chemical control strategies...
February 5, 2016: Insect Science
https://www.readbyqxmd.com/read/26846600/a-new-allele-conferring-resistance-to-lysinibacillus-sphaericus-is-detected-in-low-frequency-in-culex-quinquefasciatus-field-populations
#18
Heverly Suzany Gouveia Menezes, Karlos Diogo de Melo Chalegre, Tatiany Patrícia Romão, Cláudia Maria Fontes Oliveira, Osvaldo Pompílio de-Melo-Neto, Maria Helena Neves Lobo Silva-Filha
BACKGROUND: The Cqm1 α-glucosidase of Culex quinquefasciatus larvae acts as the midgut receptor for the binary toxin of the biolarvicide Lysinibacillus sphaericus. Mutations within the cqm1 gene can code for aberrant polypeptides that can no longer be properly expressed or bind to the toxin, leading to insect resistance. The cqm1 REC and cqm1 REC-2 alleles were identified in a laboratory selected colony and both displayed mutations that lead to equivalent phenotypes of refractoriness to L...
2016: Parasites & Vectors
https://www.readbyqxmd.com/read/26841496/from-qutn-to-bt-cotton-development-adoption-and-prospects-a-review
#19
REVIEW
W Maik, M A Abid, H M N Cheema, A A Khan, M Z Iqbal, A Qayyum, M Hanif, N Bibi, S N Yuan, A Yasmeen, A Mahmood, J Ashraf
Cotton has unique history of domestication, diversification, and utilization. Globally it is an important cash crop that provides raw material for textile industry. The story of cotton started from human civilization and the climax arrived with the efforts of developing transgenic cotton for various traits. Though conventional breeding brought steady improvement in developing resistance against biotic stresses but recent success story of gene transferfrom Bacillus thuringiensis into cotton showed game changing effects on cotton cultivation...
November 2015: T︠S︡itologii︠a︡ i Genetika
https://www.readbyqxmd.com/read/26830331/loop-replacements-with-gut-binding-peptides-in-cry1ab-domain-ii-enhanced-toxicity-against-the-brown-planthopper-nilaparvata-lugens-st%C3%A3-l
#20
Ensi Shao, Li Lin, Chen Chen, Hanze Chen, Haohan Zhuang, Songqing Wu, Li Sha, Xiong Guan, Zhipeng Huang
Bacillus thuringiensis (Bt) Cry toxins have been used widely in pest managements. However, Cry toxins are not effective against sap-sucking insects (Hemiptera), which limits the application of Bt for pest management. In order to extend the insecticidal spectrum of Bt toxins to the rice brown planthopper (BPH), Nilaparvata lugens, we modified Cry1Ab putative receptor binding domains with selected BPH gut-binding peptides (GBPs). Three surface exposed loops in the domain II of Cry1Ab were replaced with two GBPs (P2S and P1Z) respectively...
2016: Scientific Reports
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