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Light sheet microscopy

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https://www.readbyqxmd.com/read/28641108/high-speed-and-scalable-whole-brain-imaging-in-rodents-and-primates
#1
Kaoru Seiriki, Atsushi Kasai, Takeshi Hashimoto, Wiebke Schulze, Misaki Niu, Shun Yamaguchi, Takanobu Nakazawa, Ken-Ichi Inoue, Shiori Uezono, Masahiko Takada, Yuichiro Naka, Hisato Igarashi, Masato Tanuma, James A Waschek, Yukio Ago, Kenji F Tanaka, Atsuko Hayata-Takano, Kazuki Nagayasu, Norihito Shintani, Ryota Hashimoto, Yasuto Kunii, Mizuki Hino, Junya Matsumoto, Hirooki Yabe, Takeharu Nagai, Katsumasa Fujita, Toshio Matsuda, Kazuhiro Takuma, Akemichi Baba, Hitoshi Hashimoto
Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures...
June 21, 2017: Neuron
https://www.readbyqxmd.com/read/28637955/fine-structure-of-human-thoracic-duct-as-revealed-by-light-and-scanning-electron-microscopy
#2
Tomohiro Chiba, Hirokazu Narita, Hiroshi Shimoda
Little information has been available regarding microanatomy of human thoracic duct in spite of the importance for an understanding of pathophysiology in clinical medicine. The present study demonstrated a fine structure of human thoracic duct system by light and scanning electron microscopy. A number of longitudinal or spiral ridges and grooves were formed on luminal surfaces of the lymphangia and lymph sac, it likely facilitating fluent lymph flow. The endothelial cells displayed various cell shapes in compliance with their distributed regions...
2017: Biomedical Research
https://www.readbyqxmd.com/read/28637475/uptake-of-label-free-graphene-oxide-by-caco-2-cells-is-dependent-on-the-cell-differentiation-status
#3
Melanie Kucki, Liliane Diener, Nils Bohmer, Cordula Hirsch, Harald F Krug, Vincenzo Palermo, Peter Wick
BACKGROUND: Understanding the interaction of graphene-related materials (GRM) with human cells is a key to the assessment of their potential risks for human health. There is a knowledge gap regarding the potential uptake of GRM by human intestinal cells after unintended ingestion. Therefore the aim of our study was to investigate the interaction of label-free graphene oxide (GO) with the intestinal cell line Caco-2 in vitro and to shed light on the influence of the cell phenotype given by the differentiation status on cellular uptake behaviour...
June 21, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28608904/droplet-based-light-sheet-fluorescence-microscopy-for-high-throughput-sample-preparation-3-d-imaging-and-quantitative-analysis-on-a-chip
#4
Hao Jiang, Tingting Zhu, Hao Zhang, Jun Nie, Zeyi Guan, Chi-Ming Ho, Sheng Liu, Peng Fei
We report a novel fusion of droplet microfluidics and light-sheet microscopy, to achieve high-throughput sample compartmentalization, manipulation and three-dimensional imaging on a chip. This optofluidic device characterized by orthogonal plane illumination and rapid liquid handling is compact and cost-effective, and capable of preparing sample droplets with tunable size, frequency and ingredient. Each droplet flowing through the device's imaging region is self-scanned by a laser-sheet, three-dimensionally reconstructed and quantitatively analysed...
June 13, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28605364/quantitative-visualization-of-leukocyte-infiltrate-in-a-murine-model-of-fulminant-myocarditis-by-light-sheet-microscopy
#5
Linda Männ, Anika Klingberg, Matthias Gunzer, Mike Hasenberg
Light-sheet fluorescence microscopy (LSFM), in combination with chemical clearing protocols, has become the gold standard for analyzing fluorescently labelled structures in large biological specimens, and is down to cellular resolution. Meanwhile, the constant refinement of underlying protocols and the enhanced availability of specialized commercial systems enable us to investigate the microstructure of whole mouse organs and even allow for the characterization of cellular behavior in various live-cell imaging approaches...
May 31, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28600322/rac1-switching-at-the-right-time-and-location-is-essential-for-fc%C3%AE-receptor-mediated-phagosome-formation
#6
Yuka Ikeda, Katsuhisa Kawai, Akira Ikawa, Kyoko Kawamoto, Youhei Egami, Nobukazu Araki
Lamellipodia are sheet-like cell protrusions driven by actin polymerization mainly through Rac1, a GTPase molecular switch. In Fcγ receptor-mediated phagocytosis of IgG-opsonized erythrocytes (IgG-Es), Rac1 activation is required for lamellipodial extension along the surface of IgG-Es. However, the significance of Rac1 deactivation in phagosome formation is poorly understood. Our live-cell imaging and electron microscopy revealed that RAW264 macrophages expressing a constitutively active Rac1 mutant showed defects in phagocytic cup formation, while lamellipodia were formed around IgG-Es...
June 9, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28558136/multiphoton-direct-laser-writing-and-3d-imaging-of-polymeric-freestanding-architectures-for-cell-colonization
#7
Angelo Accardo, Marie-Charline Blatché, Rémi Courson, Isabelle Loubinoux, Christophe Thibault, Laurent Malaquin, Christophe Vieu
The realization of 3D architectures for the study of cell growth, proliferation, and differentiation is a task of fundamental importance for both technological and biological communities involved in the development of biomimetic cell culture environments. Here we report the fabrication of 3D freestanding scaffolds, realized by multiphoton direct laser writing and seeded with neuroblastoma cells, and their multitechnique characterization using advanced 3D fluorescence imaging approaches. The high accuracy of the fabrication process (≈200 nm) allows a much finer control of the micro- and nanoscale features compared to other 3D printing technologies based on fused deposition modeling, inkjet printing, selective laser sintering, or polyjet technology...
May 30, 2017: Small
https://www.readbyqxmd.com/read/28551226/effect-of-pulsed-light-on-activity-and-structural-changes-of-horseradish-peroxidase
#8
Bei Wang, Yanyan Zhang, Chandrasekar Venkitasamy, Bengang Wu, Zhongli Pan, Haile Ma
The objective of this research was to investigate the effects of pulsed light on the activity and structure of horseradish peroxidase in buffer solution. Enzyme residual activities were measured. Surface topography, secondary, and tertiary structures of horseradish peroxidase were determined using atomic force microscopy (AFM), Raman spectroscopy, and fluorescence spectroscopy, respectively. Results showed that a complete inactivation of horseradish peroxidase was achieved by application of 10 pulses of pulsed light treatment at an intensity of 500J/pulse...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28545837/emerging-technologies-for-prediction-of-drug-candidate-efficacy-in-the-preclinical-pipeline
#9
Denis Menshykau
The pharmaceutical industry is tackling increasingly complex multifactorial diseases, resulting in increases in research & development (R&D) costs and reductions in the success rates for drug candidates during Phase 2 and 3 clinical trials, with a lack of efficacy being the primary reason for drug candidate failure. This implies that the predictive power of current preclinical assays for drug candidate efficacy is suboptimal and, therefore, that alternatives should be developed. Here, I review emerging in vitro, imaging, and in silico technologies and discuss their potential contribution to drug efficacy assessment...
May 22, 2017: Drug Discovery Today
https://www.readbyqxmd.com/read/28537320/understanding-the-adsorptive-interactions-of-arsenate-iron-nanoparticles-with-curved-fullerene-like-sheets-in-activated-carbon-using-a-quantum-mechanics-molecular-mechanics-computational-approach
#10
Nguyen Ngoc Ha, Le Minh Cam, Nguyen Thi Thu Ha, Bee-Min Goh, Martin Saunders, Zhong-Tao Jiang, Mohammednoor Altarawneh, Bogdan Z Dlugogorski, Mohanad El-Harbawi, Chun-Yang Yin
The prevalence of global arsenic groundwater contamination has driven widespread research on developing effective treatment systems including adsorption using various sorbents. The uptake of arsenic-based contaminants onto established sorbents such as activated carbon (AC) can be effectively enhanced via immobilization/impregnation of iron-based elements on the porous AC surface. Recent suggestions that AC pores structurally consist of an eclectic mix of curved fullerene-like sheets may affect the arsenic adsorption dynamics within the AC pores and is further complicated by the presence of nano-sized iron-based elements...
June 7, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28536362/functionalisation-of-polyvinylpyrrolidone-on-gold-nanoparticles-enhances-its-anti-amyloidogenic-propensity-towards-hen-egg-white-lysozyme
#11
Tulika Das, Vidyalatha Kolli, Srijeeb Karmakar, Nandini Sarkar
Protein amyloids are characterized by aggregates that usually consist of fibres containing misfolded proteins and having a cross β-sheet conformation. These aggregates can eventually lead to several degenerative diseases like Alzheimer's disease, amyotrophic lateral sclerosis (ALS), Huntington's disease and Parkinson's disease. The present study describes the effect of chemically synthesized polyvinylpyrrolidone (PVP)-conjugated gold nanoparticles (PVP-AuNps) on hen egg white lysozyme (HEWL) amyloids. The synthesized nanoparticles have been characterized using various biophysical techniques like Ultraviolet-Visible (UV-Vis) Spectroscopy, Transmission electron microscopy (TEM), X-ray diffraction (XRD) analysis, dynamic light scattering (DLS), zeta-potential measurement and Fourier transform infrared spectroscopy (FTIR)...
May 3, 2017: Biomedicines
https://www.readbyqxmd.com/read/28531193/raman-microscopy-investigation-of-vitrification-induced-structural-damages-in-mature-bovine-oocytes
#12
Giulia Rusciano, Carolina De Canditiis, Gianluigi Zito, Marcello Rubessa, Maria Serena Roca, Rosa Carotenuto, Antonio Sasso, Bianca Gasparrini
Although oocyte cryopreservation has great potentials in the field of reproductive technologies, it still is an open challenge in the majority of domestic animals and little is known on the biochemical transformation induced by this process in the different cellular compartments. Raman micro-spectroscopy allows the non-invasive evaluation of the molecular composition of cells, based on the inelastic scattering of laser photons by vibrating molecules. The aim of this work was to assess the biochemical modifications of both the zona pellucida and cytoplasm of vitrified/warmed in vitro matured bovine oocytes at different post-warming times...
2017: PloS One
https://www.readbyqxmd.com/read/28530741/thionine-graphene-oxide-covalent-hybrid-and-its-interaction-with-light
#13
Ewelina Krzyszkowska, Justyna Walkowiak-Kulikowska, Sven Stienen, Aleksandra Wojcik
Graphene oxide sheets (GO) were covalently functionalized with thionine molecules. The obtained hybrid material, Th-GO, was characterized by means of scanning electron microscopy (SEM), Auger electron spectroscopy (AES), Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. Subsequently, the interaction of light with the free dye molecules and with dye molecules bound to the graphene oxide sheets was probed via UV-Vis spectroscopy, fluorescence spectroscopy and femtosecond pump-probe spectroscopy...
June 7, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28518097/light-sheet-based-fluorescence-microscopy-of-living-or-fixed-and-stained-tribolium-castaneum-embryos
#14
Frederic Strobl, Selina Klees, Ernst H K Stelzer
The red flour beetle Tribolium castaneum has become an important insect model organism in developmental genetics and evolutionary developmental biology. The observation of Tribolium embryos with light sheet-based fluorescence microscopy has multiple advantages over conventional widefield and confocal fluorescence microscopy. Due to the unique properties of a light sheet-based microscope, three dimensional images of living specimens can be recorded with high signal-to-noise ratios and significantly reduced photo-bleaching as well as photo-toxicity along multiple directions over periods that last several days...
April 28, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28500347/novel-fabrication-and-enhanced-photocatalytic-mb-degradation-of-hierarchical-porous-monoliths-of-moo3-nanoplates
#15
Yang Liu, Peizhong Feng, Zhang Wang, Xinyang Jiao, Farid Akhtar
Porous monoliths of MoO3 nanoplates were synthesized from ammonium molybdate (AHM) by freeze-casting and subsequent thermal treatment from 300 to 600 °C. Pure orthorhombic MoO3 phase was obtained at thermal treatment temperature of 400 °C and above. MoO3 monoliths thermally treated at 400 °C displayed bimodal pore structure, including large pore channels replicating the ice crystals and small pores from MoO3 sheets stacking. Transmission electron microscopy (TEM) images revealed that the average thicknesses of MoO3 sheet were 50 and 300 nm in porous monoliths thermally treated at 400 °C...
May 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28497438/a-qualitative-comparison-of-ten-tissue-clearing-techniques
#16
Michael Orlich, Friedemann Kiefer
The understanding of spatially complex biological systems are greatly aided by the availability of high resolution information on their tissue architecture, as is provided by optical sectioning microscopy like confocal and light sheet microscopy. In addition, genetically encoded fluorescent reporter proteins reveal tissue architecture without the need for staining procedures. Owing to opacity caused by scattering and absorption, light microscopy in tissue is limited to thin tissue layers of a few micrometers traditionally provided by histological sections...
May 12, 2017: Histology and Histopathology
https://www.readbyqxmd.com/read/28495700/visualizing-dynamic-microvillar-search-and-stabilization-during-ligand-detection-by-t-cells
#17
En Cai, Kyle Marchuk, Peter Beemiller, Casey Beppler, Matthew G Rubashkin, Valerie M Weaver, Audrey Gérard, Tsung-Li Liu, Bi-Chang Chen, Eric Betzig, Frederic Bartumeus, Matthew F Krummel
During immune surveillance, T cells survey the surface of antigen-presenting cells. In searching for peptide-loaded major histocompatibility complexes (pMHCs), they must solve a classic trade-off between speed and sensitivity. It has long been supposed that microvilli on T cells act as sensory organs to enable search, but their strategy has been unknown. We used lattice light-sheet and quantum dot-enabled synaptic contact mapping microscopy to show that anomalous diffusion and fractal organization of microvilli survey the majority of opposing surfaces within 1 minute...
May 12, 2017: Science
https://www.readbyqxmd.com/read/28494967/visualizing-calcium-flux-in-freely-moving-nematode-embryos
#18
Evan L Ardiel, Abhishek Kumar, Joseph Marbach, Ryan Christensen, Rishi Gupta, William Duncan, Jonathan S Daniels, Nico Stuurman, Daniel Colón-Ramos, Hari Shroff
The lack of physiological recordings from Caenorhabditis elegans embryos stands in stark contrast to the comprehensive anatomical and gene expression datasets already available. Using light-sheet fluorescence microscopy to address the challenges associated with functional imaging at this developmental stage, we recorded calcium dynamics in muscles and neurons and developed analysis strategies to relate activity and movement. In muscles, we found that the initiation of twitching was associated with a spreading calcium wave in a dorsal muscle bundle...
May 9, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28481540/fabrication-of-photoreactive-biocomposite-coatings-via-electric-field-assisted-assembly-of-cyanobacteria
#19
Oscar I Bernal, Bhuvnesh Bharti, Michael C Flickinger, Orlin D Velev
We report how dielectrophoresis (DEP) can be used as a tool for the fabrication of biocomposite coatings of photoreactive cyanobacteria (Synechococcus PCC7002) on flexible polyester sheets (PEs). The PE substrates were precoated by a layer-by-layer assembled film of charged polyelectrolytes. In excellent agreement between experimental data and numerical simulations, the directed assembly process driven by external electric field results in the formation of 1D chains and 2D sheets by the cells. The preassembled cyanobacteria chains and arrays became deposited on the substrate and remained in place after the electric field was turned off due to the electrostatic attraction between the negatively charged cell surfaces and the positively charged polyelectrolyte-coated PE...
May 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28471459/improving-your-four-dimensional-image-traveling-through-a-decade-of-light-sheet-based-fluorescence-microscopy-research
#20
Frederic Strobl, Alexander Schmitz, Ernst H K Stelzer
Light-sheet-based fluorescence microscopy features optical sectioning in the excitation process. This reduces phototoxicity and photobleaching by up to four orders of magnitude compared with that caused by confocal fluorescence microscopy, simplifies segmentation and quantification for three-dimensional cell biology, and supports the transition from on-demand to systematic data acquisition in developmental biology applications.
June 2017: Nature Protocols
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