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Light sheet microscopy

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https://www.readbyqxmd.com/read/28335301/facile-synthesis-of-g-c%C3%A2-n%C3%A2-nanosheets-zno-nanocomposites-with-enhanced-photocatalytic-activity-in-reduction-of-aqueous-chromium-vi-under-visible-light
#1
Xiaoya Yuan, Chao Zhou, Qiuye Jing, Qi Tang, Yuanhua Mu, An-Ke Du
Graphitic-C₃N₄ nanosheets (CN)/ZnO photocatalysts (CN/ZnO) with different CN loadings were successfully prepared via a simple precipitation-calcination in the presence of exfoliated C₃N₄ nanosheets. Their morphology and structure were thoroughly characterized by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), UV-Vis diffuse reflectance spectroscopy (DRS) and photoluminescence spectra (PL)...
September 14, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28327425/protein-drug-nanoconjugates-finding-the-alternative-proteins-as-drug-carrier
#2
Iqra Munir, Sadia Ajmal, Muhammad Raza Shah, Aftab Ahmad, Abdul Hameed, Syed Abid Ali
Present study was conducted to establish the interaction of bovine fetuin-A to validate its binding modalities with doxorubicin (Dox). Fetuin-A was purified to highest purity and monodispersity. Green synthesis of fetuin-A conjugated gold nanoparticles (F-GNPs) has been performed giving typical UV-maxima with subtle variation in fourier transform infrared spectroscopy (FTIR). Atomic force microscopy (AFM) revealed spherical shaped, polydisperse F-GNPs of varying sizes, complementing the radius of hydration (19...
March 19, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28324600/light-sheet-fluorescence-microscopy-chemical-clearing-and-labeling-protocols-for-ultramicroscopy
#3
Nina Jährling, Klaus Becker, Saiedeh Saghafi, Hans-Ulrich Dodt
Light-sheet microscopy is an effective technique in neuroscience, developmental biology, and cancer research for visualizing and analyzing cellular networks and whole organs in three dimensions. Because this technique requires specimens to be translucent they commonly have to be cleared before microscopy inspection. Here, we provide 3DISCO based protocols for preparing cleared samples of immuno-stained neural networks, lectin-labeled vascular networks, and Methoxy-X04 labeled beta-amyloid plaques in mice. 3DISCO utilizes the lipophilic solvents tetrahydrofuran (THF) and dibenzylether (DBE) for dehydration and successive clearing...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28324599/three-dimensional-live-imaging-of-filamentous-fungi-with-light-sheet-based-fluorescence-microscopy-lsfm
#4
Francesco Pampaloni, Laura Knuppertz, Andrea Hamann, Heinz D Osiewacz, Ernst H K Stelzer
We describe a method for the three-dimensional live imaging of filamentous fungi with light sheet-based fluorescence microscopy (LSFM). LSFM provides completely new opportunities to investigate the biology of fungal cells and other microorganisms with high spatial and temporal resolution. As an example, we study the established aging model Podospora anserina. The protocol explains the mounting of the live fungi for the light sheet imaging, the imaging procedure and illustrates basic image processing of data...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28322328/5d-imaging-via-light-sheet-microscopy-reveals-cell-dynamics-during-the-eye-antenna-disc-primordium-formation-in-drosophila
#5
Yu Shan Huang, Hui Yu Ku, Yun Chi Tsai, Chin Hao Chang, Sih Hua Pao, Y Henry Sun, Arthur Chiou
5D images of engrailed (en) and eye gone (eyg) gene expressions during the course of the eye-antenna disc primordium (EADP) formation of Drosophila embryos from embryonic stages 13 through 16 were recorded via light sheet microscopy and analyzed to reveal the cell dynamics involved in the development of the EADP. Detailed analysis of the time-lapsed images revealed the process of EADP formation and its invagination trajectory, which involved an inversion of the EADP anterior-posterior axis relative to the body...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28322312/imaging-tissue-mimic-with-light-sheet-microscopy-a-comparative-guideline
#6
Jordi Andilla, Raphael Jorand, Omar E Olarte, Alexandre C Dufour, Martine Cazales, Yoann L E Montagner, Romain Ceolato, Nicolas Riviere, Jean-Christophe Olivo-Marin, Pablo Loza-Alvarez, Corinne Lorenzo
Tissue mimics (TMs) on the scale of several hundred microns provide a beneficial cell culture configuration for in vitro engineered tissue and are currently under the spotlight in tissue engineering and regenerative medicine. Due to the cell density and size, TMs are fairly inaccessible to optical observation and imaging within these samples remains challenging. Light Sheet Fluorescence Microscopy (LSFM)- an emerging and attractive technique for 3D optical sectioning of large samples- appears to be a particularly well-suited approach to deal with them...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28315735/very-rapid-amyloid-fibril-formation-by-a-bacterial-lipase-in-the-absence-of-a-detectable-lag-phase
#7
Fatemeh Rashno, Khosro Khajeh, Claudia Capitini, Reza H Sajedi, Maryam Monsef Shokri, Fabrizio Chiti
The conversion of proteins from their soluble states into well-organized amyloid fibrils has received abundant attention. This process typically consists of three stages: lag, growth and plateau phases. In this study, the process of amyloid fibril formation by lipase from Pseudomonas sp. after diluting out urea was examined by Thioflavin T (ThT) fluorescence, Congo red (CR) binding, 8-anilinonaphthalene-1-sulfonic acid (ANS) binding, dynamic light scattering (DLS), circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopies, X-ray diffraction (XRD) and transmission electron microscopy (TEM)...
March 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28286999/an-assessment-of-the-importance-ofexposure-routes-to-the-uptake-and-internal-localisation-of-fluorescent-nanoparticles-in-zebrafish-danio-rerio-using-light-sheet-microscopy
#8
L M Skjolding, G Ašmonaitė, R I Jølck, T L Andresen, H Selck, A Baun, J Sturve
A major challenge in nanoecotoxicology is finding suitable methods to determine the uptake and localisation of nanoparticles on a whole-organism level. Some uptake methods have been associated with artefacts induced by sample preparation, including staining for electron microscopy. This study used light sheet microscopy (LSM) to define the uptake and localisation of fluorescently labelled nanoparticles in living organisms with minimal sample preparation. Zebrafish (Danio rerio) were exposed to fluorescent gold nanoparticles (Au NPs) and fluorescent polystyrene NPs via aqueous or dietary exposure...
March 11, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/28276783/application-of-cyclodextrins-in-antibody-microparticles-potentials-for-antibody-protection-in-spray-drying
#9
Vahid Ramezani, Alireza Vatanara, Mohammad Seyedabadi, Mohsen Nabi Meibodi, Hamed Fanaei
OBJECTIVES: Dry powder formulations are extensively used to improve the stability of antibodies. Spray drying is one of important methods for protein drying. This study investigated the effects of trehalose, hydroxypropyl beta cyclodextrin (HPBCD) and beta cyclodextrin (BCD) on the stability and particle properties of spray-dried IgG. METHODS: D-optimal design was employed for both experimental design and analysis and optimization of the variables. The size and aerodynamic behavior of particles were determined using laser light scattering and glass twin impinger, respectively...
March 1, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28274703/spines-of-the-porcupine-fish-structure-composition-and-mechanical-properties
#10
Frances Y Su, Eric A Bushong, Thomas J Deerinck, Kyungah Seo, Steven Herrera, Olivia A Graeve, David Kisailus, Vlado A Lubarda, Joanna McKittrick
This paper explores the structure, composition, and mechanical properties of porcupine fish spines for the first time. The spine was found to be composed of nanocrystalline hydroxyapatite, protein (collagen), and water using X-ray diffraction, energy-dispersive X-ray spectroscopy, and thermogravimetric analysis. Microstructures have mineralized fibrillar sheets in the longitudinal direction and in a radial orientation in the transverse direction that were observed using light and electron microscopy. Based on the images, the hierarchical structure of the spine shows both concentric and radial reinforcement...
February 28, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28270962/quantitative-three-dimensional-evaluation-of-immunofluorescence-staining-for-large-whole-mount-spheroids-with-light-sheet-microscopy
#11
I Smyrek, E H K Stelzer
Three-dimensional cell biology and histology of tissue sections strongly benefit from advanced light microscopy and optimized staining procedures to gather the full three-dimensional information. In particular, the combination of optical clearing with light sheet-based fluorescence microscopy simplifies fast high-quality imaging of thick biological specimens. However, verified in toto immunostaining protocols for large multicellular spheroids or for tissue sections have not been published. We present a method for the verification of immunostaining in three-dimensional spheroids...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28255161/multiscale-image-analysis-reveals-structural-heterogeneity-of-the-cell-microenvironment-in-homotypic-spheroids
#12
Alexander Schmitz, Sabine C Fischer, Christian Mattheyer, Francesco Pampaloni, Ernst H K Stelzer
Three-dimensional multicellular aggregates such as spheroids provide reliable in vitro substitutes for tissues. Quantitative characterization of spheroids at the cellular level is fundamental. We present the first pipeline that provides three-dimensional, high-quality images of intact spheroids at cellular resolution and a comprehensive image analysis that completes traditional image segmentation by algorithms from other fields. The pipeline combines light sheet-based fluorescence microscopy of optically cleared spheroids with automated nuclei segmentation (F score: 0...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28249360/heart-function-and-hemodynamics-analysis-for-zebrafish-embryos
#13
REVIEW
Huseyin C Yalcin, Armin Amindari, Jonathan T Butcher, Asma Althani, Magdi Yacoub
The zebrafish has emerged to become a powerful vertebrate animal model for cardiovascular research in recent years. Its advantages include easy genetic manipulation, transparency, small size, low cost, and the ability to survive without active circulation at early stages of development. Sequencing the whole genome and identifying orthologue genes with human genome enabled to induce clinically relevant cardiovascular defects via genetic approaches. Heart function and disturbed hemodynamics need to be assessed in a reliable manner for these disease models in order to reveal mechanobiology of induced defects...
March 1, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28235719/hierarchical-layered-ni3s2-graphene-hybrid-composites-for-efficient-photocatalytic-reduction-of-cr-vi
#14
Pinpin Hu, Xinjuan Liu, Baibai Liu, Lei Li, Wei Qin, Huangze Yu, Sisi Zhong, Yan Li, Zhenxing Ren, Mengliang Wang
Exploring visible light responsive media remains a challenge for solar energy photocatalysis applications. We report the Ni3S2-reduced graphene oxide (NG) hybrid composites with sheet-on-sheet structures synthesized by a facile microwave-assisted method. Their morphology, structure and photocatalytic activity in the reduction of Cr(VI) were characterized by scanning electron microscopy, X-ray diffraction, UV-Vis absorption spectroscopy, and electrochemical impedance spectra, respectively. The results show that NG hybrid composites show excellent visible light photocatalytic activity in the reduction of Cr(VI) compared to pure Ni3S2...
January 24, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28230156/drebrin-mediated-microtubule-actomyosin-coupling-steers-cerebellar-granule-neuron-nucleokinesis-and-migration-pathway-selection
#15
Niraj Trivedi, Daniel R Stabley, Blake Cain, Danielle Howell, Christophe Laumonnerie, Joseph S Ramahi, Jamshid Temirov, Ryan A Kerekes, Phillip R Gordon-Weeks, David J Solecki
Neuronal migration from a germinal zone to a final laminar position is essential for the morphogenesis of neuronal circuits. While it is hypothesized that microtubule-actomyosin crosstalk is required for a neuron's 'two-stroke' nucleokinesis cycle, the molecular mechanisms controlling such crosstalk are not defined. By using the drebrin microtubule-actin crosslinking protein as an entry point into the cerebellar granule neuron system in combination with super-resolution microscopy, we investigate how these cytoskeletal systems interface during migration...
February 23, 2017: Nature Communications
https://www.readbyqxmd.com/read/28208588/depicting-binding-mediated-translocation-of-hiv-1-tat-peptides-in-living-cells-with-nanoscale-pens-of-tat-conjugated-quantum-dots
#16
Chien Y Lin, Jung Y Huang, Leu-Wei Lo
Cell-penetrating peptides (CPPs) can translocate across cell membranes, and thus have great potential for the cellular delivery of macromolecular cargoes. However, the mechanism of this cellular uptake process is not yet fully understood. In this study, a time-lapse single-particle light-sheet microscopy technique was implemented to obtain a parallel visualization of the translocating process of individual human immunodeficiency virus 1 (HIV-1) transactivator of transcription (Tat) peptide conjugated quantum dots (TatP-QDs) in complex cellular terrains...
February 10, 2017: Sensors
https://www.readbyqxmd.com/read/28194956/direct-imaging-of-single-plasmonic-metal-nanoparticles-in-capillary-with-laser-light-sheet-scattering-imaging
#17
Xuan Cao, Jingjing Feng, Qi Pan, Bin Xiong, Yan He, Edward S Yeung
Understanding the heterogeneous distribution of the physical and chemical properties of plasmonic metal nanoparticles is fundamentally important to their basic and applied research. Traditionally, they are obtained either indirectly via bulk spectroscopic measurements plus electron microscopic characterizations or through single molecule/particle imaging of nanoparticles immobilized on planar substrates. In this study, by using light-sheet scattering microscopy with a supercontinuum white laser, highly sensitive imaging of individual metal nanoparticles (MNPs) flowing inside a capillary, driven by either pressure or electric field, was achieved for the first time...
February 24, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28165052/light-sheet-fluorescence-imaging-to-localize-cardiac-lineage-and-protein-distribution
#18
Yichen Ding, Juhyun Lee, Jianguo Ma, Kevin Sung, Tomohiro Yokota, Neha Singh, Mojdeh Dooraghi, Parinaz Abiri, Yibin Wang, Rajan P Kulkarni, Atsushi Nakano, Thao P Nguyen, Peng Fei, Tzung K Hsiai
Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm(3) in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution...
February 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28132409/selectable-light-sheet-uniformity-using-tuned-axial-scanning
#19
Martí Duocastella, Craig B Arnold, Jason Puchalla
Light-sheet fluorescence microscopy (LSFM) is an optical sectioning technique capable of rapid three-dimensional (3D) imaging of a wide range of specimens with reduced phototoxicity and superior background rejection. However, traditional light-sheet generation approaches based on elliptical or circular Gaussian beams suffer an inherent trade-off between light-sheet thickness and area over which this thickness is preserved. Recently, an increase in light-sheet uniformity was demonstrated using rapid biaxial Gaussian beam scanning along the lateral and beam propagation directions...
February 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28118055/the-role-of-mitochondria-oxidative-stress-and-the-radical-binding-protein-a1m-in-cultured-porcine-retina
#20
Bo Åkerström, Martin Cederlund, Jesper Bergwik, Oscar Manouchehrian, Karin Arnér, Ingrid Holmgren Taylor, Fredrik Ghosh, Linnéa Taylor
PURPOSE: The purpose of this study was to explore the relationship between oxidative stress, antioxidant defense, mitochondrial structure, and biomechanical tissue support in the isolated porcine retina. METHODS: Full-thickness retinal sheets were isolated from adult porcine eyes. Retinas were cultured for 2 or 48 h using (1) a previously established low-support explant protocol with photoreceptors positioned against the culture membrane (porous polycarbonate) or (2) a high-support procedure developed by our group, apposing the Müller cell endfeet and inner limiting membrane against the membrane...
January 24, 2017: Current Eye Research
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