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Light sheet microscopy

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https://www.readbyqxmd.com/read/28724968/ce-3-ion-surface-oxygen-vacancy-and-visible-light-induced-photocatalytic-dye-degradation-and-photocapacitive-performance-of-ceo2-graphene-nanostructures
#1
Mohammad Ehtisham Khan, Mohammad Mansoob Khan, Moo Hwan Cho
Cerium oxide nanoparticles (CeO2 NPs) were fabricated and grown on graphene sheets using a facile, low cost hydrothermal approach and subsequently characterized using different standard characterization techniques. X-ray photoelectron spectroscopy and electron paramagnetic resonance revealed the changes in surface states, composition, changes in Ce(4+) to Ce(3+) ratio, and other defects. Transmission electron microscopy (TEM) and high resolution TEM revealed that the fabricated CeO2 NPs to be spherical with particle size of ~10-12 nm...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28717817/quantifying-three-dimensional-rodent-retina-vascular-development-using-optical-tissue-clearing-and-light-sheet-microscopy
#2
Jasmine N Singh, Taylor M Nowlin, Gregory J Seedorf, Steven H Abman, Douglas P Shepherd
No abstract text is available yet for this article.
July 1, 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28675620/site-specific-sers-assay-for-survivin-protein-dimer-from-ensemble-experiments-to-correlative-single-particle-imaging
#3
Jörg Wissler, Sandra Bäcker, Alessandro Feis, Shirley K Knauer, Sebastian Schlücker
An assay for Survivin, a small dimeric protein which functions as modulator of apoptosis and cell division and serves as a promising diagnostic biomarker for different types of cancer, is presented. The assay is based on switching on surface-enhanced Raman scattering (SERS) upon incubation of the Survivin protein dimer with Raman reporter-labeled gold nanoparticles (AuNP). Site-specificity is achieved by complexation of nickel-chelated N-nitrilo-triacetic acid (Ni-NTA) anchors on the particle surface by multiple histidines (His6 -tag) attached to each C-terminus of the centrosymmetric protein dimer...
July 4, 2017: Small
https://www.readbyqxmd.com/read/28671211/glucose-directs-amyloid-beta-into-membrane-active-oligomers
#4
Niraja Kedia, Michael Almisry, Jan Bieschke
Oligomeric amyloid-β 1-42 (Aβ-42) peptides are considered to be the most toxic species connected to the occurrence of Alzheimer's disease. However, not all aggregation conditions promote oligomer formation in vitro, raising the question whether oligomer formation in vivo also requires a specific suitable cellular environment. We recently found that interaction with neuronal membranes initiates aggregation of Aβ-42 and neuronal uptake. Our data suggest that small molecules in the extracellular space can facilitate the formation of membrane-active Aβ-42 oligomers...
July 21, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28661225/different-amyloid-aggregation-of-smooth-muscles-titin-in-vitro
#5
Elmira I Yakupova, Ivan M Vikhlyantsev, Liya G Bobyleva, Nikita V Penkov, Alexander A Timchenko, Maria A Timchenko, Gennady A Enin, Sergei S Khutzian, Olga M Selivanova, Alexander G Bobylev
A comparative study of amyloid properties of the aggregates of smooth muscle titin (SMT) from chicken gizzard was carried out. These aggregates were formed in two solutions: 0.15 M glycine-KOH, pH 7.2-7.4 (SMT(Gly)) and 0.2 M KCl, 10 mM imidazole, pH 7.0 (SMT(KCl)). Electron microscopy data showed that SMT aggregates has an amorphous structure in both cases. The results of atomic-force microscopy demonstrated slight differences in morphology in two types of aggregates. The SMT(Gly) aggregates were represented as branching chains, composed of spherical aggregates approximately 300-500 nm in diameter and up to 35 nm in height...
June 29, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28648678/single-plane-illumination-microscopy-as-a-tool-for-studying-nucleome-dynamics
#6
Jörg Langowski
Single plane illumination microscopy (SPIM) is a new optical method that has become extremely important in recent years. It is based on the formation of a "light slice" in the specimen in which fluorescently tagged molecules are observed. The spatial resolution is close to that of confocal optics, but without the disadvantages inherent to scanning or high laser irradiation doses. A recent development is light sheet fluctuation microscopy, which exploits the dynamic information contained in the fluorescence intensity fluctuations of each image pixel...
June 23, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28641108/high-speed-and-scalable-whole-brain-imaging-in-rodents-and-primates
#7
Kaoru Seiriki, Atsushi Kasai, Takeshi Hashimoto, Wiebke Schulze, Misaki Niu, Shun Yamaguchi, Takanobu Nakazawa, Ken-Ichi Inoue, Shiori Uezono, Masahiko Takada, Yuichiro Naka, Hisato Igarashi, Masato Tanuma, James A Waschek, Yukio Ago, Kenji F Tanaka, Atsuko Hayata-Takano, Kazuki Nagayasu, Norihito Shintani, Ryota Hashimoto, Yasuto Kunii, Mizuki Hino, Junya Matsumoto, Hirooki Yabe, Takeharu Nagai, Katsumasa Fujita, Toshio Matsuda, Kazuhiro Takuma, Akemichi Baba, Hitoshi Hashimoto
Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures...
June 21, 2017: Neuron
https://www.readbyqxmd.com/read/28637955/fine-structure-of-human-thoracic-duct-as-revealed-by-light-and-scanning-electron-microscopy
#8
Tomohiro Chiba, Hirokazu Narita, Hiroshi Shimoda
Little information has been available regarding microanatomy of human thoracic duct in spite of the importance for an understanding of pathophysiology in clinical medicine. The present study demonstrated a fine structure of human thoracic duct system by light and scanning electron microscopy. A number of longitudinal or spiral ridges and grooves were formed on luminal surfaces of the lymphangia and lymph sac, it likely facilitating fluent lymph flow. The endothelial cells displayed various cell shapes in compliance with their distributed regions...
2017: Biomedical Research
https://www.readbyqxmd.com/read/28637475/uptake-of-label-free-graphene-oxide-by-caco-2-cells-is-dependent-on-the-cell-differentiation-status
#9
Melanie Kucki, Liliane Diener, Nils Bohmer, Cordula Hirsch, Harald F Krug, Vincenzo Palermo, Peter Wick
BACKGROUND: Understanding the interaction of graphene-related materials (GRM) with human cells is a key to the assessment of their potential risks for human health. There is a knowledge gap regarding the potential uptake of GRM by human intestinal cells after unintended ingestion. Therefore the aim of our study was to investigate the interaction of label-free graphene oxide (GO) with the intestinal cell line Caco-2 in vitro and to shed light on the influence of the cell phenotype given by the differentiation status on cellular uptake behaviour...
June 21, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28608904/droplet-based-light-sheet-fluorescence-microscopy-for-high-throughput-sample-preparation-3-d-imaging-and-quantitative-analysis-on-a-chip
#10
Hao Jiang, Tingting Zhu, Hao Zhang, Jun Nie, Zeyi Guan, Chi-Ming Ho, Sheng Liu, Peng Fei
We report a novel fusion of droplet microfluidics and light-sheet microscopy, to achieve high-throughput sample compartmentalization, manipulation and three-dimensional imaging on a chip. This optofluidic device characterized by orthogonal plane illumination and rapid liquid handling is compact and cost-effective, and capable of preparing sample droplets with tunable size, frequency and ingredient. Each droplet flowing through the device's imaging region is self-scanned by a laser-sheet, three-dimensionally reconstructed and quantitatively analysed...
June 13, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28605364/quantitative-visualization-of-leukocyte-infiltrate-in-a-murine-model-of-fulminant-myocarditis-by-light-sheet-microscopy
#11
Linda Männ, Anika Klingberg, Matthias Gunzer, Mike Hasenberg
Light-sheet fluorescence microscopy (LSFM), in combination with chemical clearing protocols, has become the gold standard for analyzing fluorescently labelled structures in large biological specimens, and is down to cellular resolution. Meanwhile, the constant refinement of underlying protocols and the enhanced availability of specialized commercial systems enable us to investigate the microstructure of whole mouse organs and even allow for the characterization of cellular behavior in various live-cell imaging approaches...
May 31, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28600322/rac1-switching-at-the-right-time-and-location-is-essential-for-fc%C3%AE-receptor-mediated-phagosome-formation
#12
Yuka Ikeda, Katsuhisa Kawai, Akira Ikawa, Kyoko Kawamoto, Youhei Egami, Nobukazu Araki
Lamellipodia are sheet-like cell protrusions driven by actin polymerization mainly through Rac1, a GTPase molecular switch. In Fcγ receptor-mediated phagocytosis of IgG-opsonized erythrocytes (IgG-Es), Rac1 activation is required for lamellipodial extension along the surface of IgG-Es. However, the significance of Rac1 deactivation in phagosome formation is poorly understood. Our live-cell imaging and electron microscopy revealed that RAW264 macrophages expressing a constitutively active Rac1 mutant showed defects in phagocytic cup formation, while lamellipodia were formed around IgG-Es...
June 9, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28558136/multiphoton-direct-laser-writing-and-3d-imaging-of-polymeric-freestanding-architectures-for-cell-colonization
#13
Angelo Accardo, Marie-Charline Blatché, Rémi Courson, Isabelle Loubinoux, Christophe Thibault, Laurent Malaquin, Christophe Vieu
The realization of 3D architectures for the study of cell growth, proliferation, and differentiation is a task of fundamental importance for both technological and biological communities involved in the development of biomimetic cell culture environments. Here we report the fabrication of 3D freestanding scaffolds, realized by multiphoton direct laser writing and seeded with neuroblastoma cells, and their multitechnique characterization using advanced 3D fluorescence imaging approaches. The high accuracy of the fabrication process (≈200 nm) allows a much finer control of the micro- and nanoscale features compared to other 3D printing technologies based on fused deposition modeling, inkjet printing, selective laser sintering, or polyjet technology...
May 30, 2017: Small
https://www.readbyqxmd.com/read/28551226/effect-of-pulsed-light-on-activity-and-structural-changes-of-horseradish-peroxidase
#14
Bei Wang, Yanyan Zhang, Chandrasekar Venkitasamy, Bengang Wu, Zhongli Pan, Haile Ma
The objective of this research was to investigate the effects of pulsed light on the activity and structure of horseradish peroxidase in buffer solution. Enzyme residual activities were measured. Surface topography, secondary, and tertiary structures of horseradish peroxidase were determined using atomic force microscopy (AFM), Raman spectroscopy, and fluorescence spectroscopy, respectively. Results showed that a complete inactivation of horseradish peroxidase was achieved by application of 10 pulses of pulsed light treatment at an intensity of 500J/pulse...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28545837/emerging-technologies-for-prediction-of-drug-candidate-efficacy-in-the-preclinical-pipeline
#15
Denis Menshykau
The pharmaceutical industry is tackling increasingly complex multifactorial diseases, resulting in increases in research & development (R&D) costs and reductions in the success rates for drug candidates during Phase 2 and 3 clinical trials, with a lack of efficacy being the primary reason for drug candidate failure. This implies that the predictive power of current preclinical assays for drug candidate efficacy is suboptimal and, therefore, that alternatives should be developed. Here, I review emerging in vitro, imaging, and in silico technologies and discuss their potential contribution to drug efficacy assessment...
May 22, 2017: Drug Discovery Today
https://www.readbyqxmd.com/read/28537320/understanding-the-adsorptive-interactions-of-arsenate-iron-nanoparticles-with-curved-fullerene-like-sheets-in-activated-carbon-using-a-quantum-mechanics-molecular-mechanics-computational-approach
#16
Nguyen Ngoc Ha, Le Minh Cam, Nguyen Thi Thu Ha, Bee-Min Goh, Martin Saunders, Zhong-Tao Jiang, Mohammednoor Altarawneh, Bogdan Z Dlugogorski, Mohanad El-Harbawi, Chun-Yang Yin
The prevalence of global arsenic groundwater contamination has driven widespread research on developing effective treatment systems including adsorption using various sorbents. The uptake of arsenic-based contaminants onto established sorbents such as activated carbon (AC) can be effectively enhanced via immobilization/impregnation of iron-based elements on the porous AC surface. Recent suggestions that AC pores structurally consist of an eclectic mix of curved fullerene-like sheets may affect the arsenic adsorption dynamics within the AC pores and is further complicated by the presence of nano-sized iron-based elements...
June 7, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28536362/functionalisation-of-polyvinylpyrrolidone-on-gold-nanoparticles-enhances-its-anti-amyloidogenic-propensity-towards-hen-egg-white-lysozyme
#17
Tulika Das, Vidyalatha Kolli, Srijeeb Karmakar, Nandini Sarkar
Protein amyloids are characterized by aggregates that usually consist of fibres containing misfolded proteins and having a cross β-sheet conformation. These aggregates can eventually lead to several degenerative diseases like Alzheimer's disease, amyotrophic lateral sclerosis (ALS), Huntington's disease and Parkinson's disease. The present study describes the effect of chemically synthesized polyvinylpyrrolidone (PVP)-conjugated gold nanoparticles (PVP-AuNps) on hen egg white lysozyme (HEWL) amyloids. The synthesized nanoparticles have been characterized using various biophysical techniques like Ultraviolet-Visible (UV-Vis) Spectroscopy, Transmission electron microscopy (TEM), X-ray diffraction (XRD) analysis, dynamic light scattering (DLS), zeta-potential measurement and Fourier transform infrared spectroscopy (FTIR)...
May 3, 2017: Biomedicines
https://www.readbyqxmd.com/read/28531193/raman-microscopy-investigation-of-vitrification-induced-structural-damages-in-mature-bovine-oocytes
#18
Giulia Rusciano, Carolina De Canditiis, Gianluigi Zito, Marcello Rubessa, Maria Serena Roca, Rosa Carotenuto, Antonio Sasso, Bianca Gasparrini
Although oocyte cryopreservation has great potentials in the field of reproductive technologies, it still is an open challenge in the majority of domestic animals and little is known on the biochemical transformation induced by this process in the different cellular compartments. Raman micro-spectroscopy allows the non-invasive evaluation of the molecular composition of cells, based on the inelastic scattering of laser photons by vibrating molecules. The aim of this work was to assess the biochemical modifications of both the zona pellucida and cytoplasm of vitrified/warmed in vitro matured bovine oocytes at different post-warming times...
2017: PloS One
https://www.readbyqxmd.com/read/28530741/thionine-graphene-oxide-covalent-hybrid-and-its-interaction-with-light
#19
Ewelina Krzyszkowska, Justyna Walkowiak-Kulikowska, Sven Stienen, Aleksandra Wojcik
Graphene oxide sheets (GO) were covalently functionalized with thionine molecules. The obtained hybrid material, Th-GO, was characterized by means of scanning electron microscopy (SEM), Auger electron spectroscopy (AES), Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. Subsequently, the interaction of light with the free dye molecules and with dye molecules bound to the graphene oxide sheets was probed via UV-Vis spectroscopy, fluorescence spectroscopy and femtosecond pump-probe spectroscopy...
June 7, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28518097/light-sheet-based-fluorescence-microscopy-of-living-or-fixed-and-stained-tribolium-castaneum-embryos
#20
Frederic Strobl, Selina Klees, Ernst H K Stelzer
The red flour beetle Tribolium castaneum has become an important insect model organism in developmental genetics and evolutionary developmental biology. The observation of Tribolium embryos with light sheet-based fluorescence microscopy has multiple advantages over conventional widefield and confocal fluorescence microscopy. Due to the unique properties of a light sheet-based microscope, three dimensional images of living specimens can be recorded with high signal-to-noise ratios and significantly reduced photo-bleaching as well as photo-toxicity along multiple directions over periods that last several days...
April 28, 2017: Journal of Visualized Experiments: JoVE
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