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Light sheet microscopy

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https://www.readbyqxmd.com/read/27886401/not-all-that-glitters-is-gold-electron-microscopy-study-on-uptake-of-gold-nanoparticles-in-daphnia-magna-and-related-artefacts
#1
Louise Helene Søgaard Jensen, Lars Michael Skjolding, Amalie Thit, Sara Nørgaard Sørensen, Carsten Købler, Kristian Mølhave, Anders Baun
Increasing use of engineered nanoparticles has led to extensive research into their potential hazards to the environment and human health. Cellular uptake from the gut is sparsely investigated and microscopy techniques applied for uptake studies can result in misinterpretations. Various microscopy techniques are used to investigate internalization of 10 nm gold nanoparticles in Daphnia magna gut lumen and gut epithelial cells upon 24h exposure and outline potential artefacts, i.e. high contract precipitates from sample preparation related to these techniques...
November 25, 2016: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/27886235/time-lapse-3-d-measurements-of-a-glucose-biosensor-in-multicellular-spheroids-by-light-sheet-fluorescence-microscopy-in-commercial-96-well-plates
#2
Vincent Maioli, George Chennell, Hugh Sparks, Tobia Lana, Sunil Kumar, David Carling, Alessandro Sardini, Chris Dunsby
Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-D biological cultures in commercially available glass-bottomed 96-well plates using a stage-scanning OPM approach (ssOPM). Time-lapse 3-D imaging of multicellular spheroids expressing a glucose Förster resonance energy transfer (FRET) biosensor is demonstrated in 16 fields of view with image acquisition at 10 minute intervals...
November 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27870352/membranous-support-for-eyes-of-strepsirrhine-primates-and-fruit-bats
#3
Brianna M Harvey, Kunwar P Bhatnagar, Robert J Schenck, Alfred L Rosenberger, Susan J Rehorek, Anne M Burrows, Valerie B DeLeon, Timothy D Smith
Living primates have relatively large eyes and support orbital tissues with a postorbital bar (POB) and/or septum. Some mammals with large eyes lack a POB, and presumably rely on soft tissues. Here, we examined the orbits of four species of strepsirrhine primates (Galagidae, Cheirogaleidae) and three species of fruit bats (Pteropodidae). Microdissection and light microscopy were employed to identify support structures of the orbit. In bats and primates, there are two layers of fascial sheets that border the eye laterally...
December 2016: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/27869673/3d-visualization-of-developmental-toxicity-of-2-4-6-trinitrotoluene-in-zebrafish-embryogenesis-using-light-sheet-microscopy
#4
Juneyong Eum, Jina Kwak, Hee Joung Kim, Seoyoung Ki, Kooyeon Lee, Ahmed A Raslan, Ok Kyu Park, Md Ashraf Uddin Chowdhury, Song Her, Yun Kee, Seung-Hae Kwon, Byung Joon Hwang
Environmental contamination by trinitrotoluene is of global concern due to its widespread use in military ordnance and commercial explosives. Despite known long-term persistence in groundwater and soil, the toxicological profile of trinitrotoluene and other explosive wastes have not been systematically measured using in vivo biological assays. Zebrafish embryos are ideal model vertebrates for high-throughput toxicity screening and live in vivo imaging due to their small size and transparency during embryogenesis...
November 17, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27867712/enhancement-of-image-quality-and-imaging-depth-with-airy-light-sheet-microscopy-in-cleared-and-non-cleared-neural-tissue
#5
Jonathan Nylk, Kaley McCluskey, Sanya Aggarwal, Javier A Tello, Kishan Dholakia
We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations...
October 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27865970/insights-into-amyloid-like-aggregation-of-h2-region-of-the-c-terminal-domain-of-nucleophosmin
#6
Anna Russo, Carlo Diaferia, Sara La Manna, Cinzia Giannini, Teresa Sibillano, Antonella Accardo, Giancarlo Morelli, Ettore Novellino, Daniela Marasco
Nucleophosmin (NPM1) is a multifunctional protein involved in a variety of biological processes including the pathogenesis of several human malignancies and is the most frequently mutated gene in Acute Myeloid Leukemia (AML). To deepen the role of protein regions in its biological activities, lately we reported on the structural behavior of dissected C-terminal domain (CTD) helical fragments. Unexpectedly the H2 (residues 264-277) and H3 AML-mutated regions showed a remarkable tendency to form amyloid-like assemblies with fibrillar morphology and β-sheet structure that resulted as toxic when exposed to human neuroblastoma cells...
November 17, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27862624/gadolinium-containing-telechelic-peg-polymers-end-capped-by-di-phenylalanine-motives-as-potential-supramolecular-mri-contrast-agents
#7
Carlo Diaferia, Eliana Gianolio, Antonella Accardo, Giancarlo Morelli
Telechelic PEG-polymers end-capped by diphenylalanine (FF) motives and containing a DOTA-Gd complex, bound on a lysine side chain at the centre of peptide moiety, are studied for their assembling properties and for the relaxometric behavior. The observed variations in terms of relaxivity are correlated to the assembling properties of the aggregates by using several techniques: fluorescence, Circular Dichroism (CD) and Fourier Transform Infrared (FTIR) for aggregation tendency and secondary structure determination; Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM) for morphological definition...
November 15, 2016: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/27860053/exploring%C3%A2-morphological-and-biochemical-linkages%C3%A2-in-fungal-growth%C3%A2-with-label-free-light-sheet-microscopy-and-raman-spectroscopy
#8
Soumik Siddhanta, Santosh Paidi, Kathryn Bushley, Ram Prasad, Ishan Barman
Imaging tip growth in fungal hyphae is highly warranted to unravel the molecular mechanism of this extraordinarily precise and localized phenomenon. In situ probing of fungal cultures, however, have been challenging due to their inherent complexity and light penetration issues associated with conventional optical imaging. In this work, we report a label-free approach using a combination of light sheet microscopy and Raman spectroscopy to obtain concomitant morphological and biochemical information from the growing specimen...
November 15, 2016: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/27853250/labeling-and-analysis-of-chicken-taste-buds-using-molecular-markers-in-oral-epithelial-sheets
#9
Prasangi Rajapaksha, Zhonghou Wang, Nandakumar Venkatesan, Kayvan F Tehrani, Jason Payne, Raymond L Swetenburg, Fuminori Kawabata, Shoji Tabata, Luke J Mortensen, Steven L Stice, Robert Beckstead, Hong-Xiang Liu
In chickens, the sensory organs for taste are the taste buds in the oral cavity, of which there are ~240-360 in total number as estimated by scanning electron microscopy (SEM). There is not an easy way to visualize all taste buds in chickens. Here, we report a highly efficient method for labeling chicken taste buds in oral epithelial sheets using the molecular markers Vimentin and α-Gustducin. Immediate tissue fixation following incubation with sub-epithelially injected proteases enabled us to peel off whole epithelial sheets, leaving the shape and integrity of the tissue intact...
November 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27829356/alternative-evolution-of-a-spheroidal-colony-in-volvocine-algae-developmental-analysis-of-embryogenesis-in-astrephomene-volvocales-chlorophyta
#10
Shota Yamashita, Yoko Arakaki, Hiroko Kawai-Toyooka, Akira Noga, Masafumi Hirono, Hisayoshi Nozaki
BACKGROUND: Volvocine algae, which range from the unicellular Chlamydomonas to the multicellular Volvox with a germ-soma division of labor, are a model for the evolution of multicellularity. Within this group, the spheroidal colony might have evolved in two independent lineages: Volvocaceae and the goniacean Astrephomene. Astrephomene produces spheroidal colonies with posterior somatic cells. The feature that distinguishes Astrephomene from the volvocacean algae is lack of inversion during embryogenesis; the volvocacean embryo undergoes inversion after successive divisions to orient flagella toward the outside...
November 9, 2016: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/27824107/structural-changes-induced-by-acidic-ph-in-human-apolipoprotein-b-100
#11
José A Fernández-Higuero, Asier Benito-Vicente, Aitor Etxebarria, José Carlos G Milicua, Helena Ostolaza, José L R Arrondo, Cesar Martín
Acidification in the endosome causes lipoprotein release by promoting a conformational change in the LDLR allowing its recycling and degradation of LDL. Notwithstanding conformational changes occurring in the LDLR have expanded considerably, structural changes occurring in LDL particles have not been fully explored yet. The objectives of the present work were to study structural changes occurring in apoB100 by infrared spectroscopy (IR) and also LDL size and morphology by dynamic light scattering (DLS) and electron microscopy (EM) at both pH 7...
November 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27810916/independent-modes-of-ganglion-cell-translocation-ensure-correct-lamination-of-the-zebrafish-retina
#12
Jaroslav Icha, Christiane Kunath, Mauricio Rocha-Martins, Caren Norden
The arrangement of neurons into distinct layers is critical for neuronal connectivity and function. During development, most neurons move from their birthplace to the appropriate layer, where they polarize. However, kinetics and modes of many neuronal translocation events still await exploration. In this study, we investigate retinal ganglion cell (RGC) translocation across the embryonic zebrafish retina. After completing their translocation, RGCs establish the most basal retinal layer where they form the optic nerve...
October 24, 2016: Journal of Cell Biology
https://www.readbyqxmd.com/read/27805674/light-sheet-fluorescence-microscopy-using-high-speed-structured-and-pivoting-illumination
#13
Ryosuke Itoh, Joseph Russell Landry, Stephen Sanborn Hamann, Olav Solgaard
We show that light sheet fluorescence microscopy with structured and pivoting illumination enables fast image acquisition and improved image quality. A one-dimensional spatial light phase modulator is used to control the illumination profile at high speed. To demonstrate the features of the system, we image fluorescent beads and biological samples, successfully obtaining optically sectioned images with higher contrast using structured illumination and with reduced shadowing effects using pivoting illumination...
November 1, 2016: Optics Letters
https://www.readbyqxmd.com/read/27798562/adaptive-light-sheet-microscopy-for-long-term-high-resolution-imaging-in-living-organisms
#14
Loïc A Royer, William C Lemon, Raghav K Chhetri, Yinan Wan, Michael Coleman, Eugene W Myers, Philipp J Keller
Optimal image quality in light-sheet microscopy requires a perfect overlap between the illuminating light sheet and the focal plane of the detection objective. However, mismatches between the light-sheet and detection planes are common owing to the spatiotemporally varying optical properties of living specimens. Here we present the AutoPilot framework, an automated method for spatiotemporally adaptive imaging that integrates (i) a multi-view light-sheet microscope capable of digitally translating and rotating light-sheet and detection planes in three dimensions and (ii) a computational method that continuously optimizes spatial resolution across the specimen volume in real time...
December 2016: Nature Biotechnology
https://www.readbyqxmd.com/read/27784051/stripe-artifact-elimination-based-on-nonsubsampled-contourlet-transform-for-light-sheet-fluorescence-microscopy
#15
Xiao Liang, Yali Zang, Di Dong, Liwen Zhang, Mengjie Fang, Xin Yang, Alicia Arranz, Jorge Ripoll, Hui Hui, Jie Tian
No abstract text is available yet for this article.
October 1, 2016: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/27779273/light-sheet-microscopy-by-confocal-line-scanning-of-dual-bessel-beams
#16
Pengfei Zhang, Mary E Phipps, Peter M Goodwin, James H Werner
No abstract text is available yet for this article.
October 1, 2016: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/27761486/simultaneous-multiview-capture-and-fusion-improves-spatial-resolution-in-wide-field-and-light-sheet-microscopy
#17
Yicong Wu, Panagiotis Chandris, Peter W Winter, Edward Y Kim, Valentin Jaumouillé, Abhishek Kumar, Min Guo, Jacqueline M Leung, Corey Smith, Ivan Rey-Suarez, Huafeng Liu, Clare M Waterman, Kumaran S Ramamurthi, Patrick J La Riviere, Hari Shroff
Most fluorescence microscopes are inefficient, collecting only a small fraction of the emitted light at any instant. Besides wasting valuable signal, this inefficiency also reduces spatial resolution and causes imaging volumes to exhibit significant resolution anisotropy. We describe microscopic and computational techniques that address these problems by simultaneously capturing and subsequently fusing and deconvolving multiple specimen views. Unlike previous methods that serially capture multiple views, our approach improves spatial resolution without introducing any additional illumination dose or compromising temporal resolution relative to conventional imaging...
August 20, 2016: Optica
https://www.readbyqxmd.com/read/27733696/identifying-the-necrotic-zone-boundary-in-tumour-spheroids-with-pair-correlation-functions
#18
S Dini, B J Binder, S C Fischer, C Mattheyer, A Schmitz, E H K Stelzer, N G Bean, J E F Green
Automatic identification of the necrotic zone boundary is important in the assessment of treatments on in vitro tumour spheroids. This has been difficult especially when the difference in cell density between the necrotic and viable zones of a tumour spheroid is small. To help overcome this problem, we developed novel one-dimensional pair-correlation functions (PCFs) to provide quantitative estimates of the radial distance of the necrotic zone boundary from the centre of a tumour spheroid. We validate our approach on synthetic tumour spheroids in which the position of the necrotic zone boundary is known a priori It is then applied to nine real tumour spheroids imaged with light sheet-based fluorescence microscopy...
October 2016: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/27733125/distinct-shape-shifting-regimes-of-bowl-shaped-cell-sheets-embryonic-inversion-in-the-multicellular-green-alga-pleodorina
#19
Stephanie Höhn, Armin Hallmann
BACKGROUND: The multicellular volvocine alga Pleodorina is intermediate in organismal complexity between its unicellular relative, Chlamydomonas, and its multicellular relative, Volvox, which shows complete division of labor between different cell types. The volvocine green microalgae form a group of genera closely related to the genus Volvox within the order Volvocales (Chlorophyta). Embryos of multicellular volvocine algae consist of a cellular monolayer that, depending on the species, is either bowl-shaped or comprises a sphere...
October 13, 2016: BMC Developmental Biology
https://www.readbyqxmd.com/read/27730582/sample-preparation-and-mounting-of-drosophila-embryos-for-multiview-light-sheet-microscopy
#20
Christopher Schmied, Pavel Tomancak
Light sheet fluorescent microscopy (LSFM), and in particular its most widespread flavor Selective Plane Illumination Microscopy (SPIM), promises to provide unprecedented insights into developmental dynamics of entire living systems. By combining minimal photo-damage with high imaging speed and sample mounting tailored toward the needs of the specimen, it enables in toto imaging of embryogenesis with high spatial and temporal resolution. Drosophila embryos are particularly well suited for SPIM imaging because the volume of the embryo does not change from the single cell embryo to the hatching larva...
2016: Methods in Molecular Biology
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