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Light sheet microscopy

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https://www.readbyqxmd.com/read/28821593/light-sheet-microscopy-imaging-of-light-absorption-and-photosynthesis-distribution-in-plant-tissue
#1
Mads Lichtenberg, Erik Christian Løvbjerg Trampe, Thomas Craig Vogelmann, Michael Kühl
In vivo variable-chlorophyll-fluorescence measurements of PSII quantum yields in optically dense systems are complicated by steep tissue light gradients due to scattering and absorption. Consequently, externally measured effective PSII quantum yields may be composed of signals derived from cells differentially exposed to actinic light, where cells located deeper inside tissues receive lower irradiance than cells closer to the surface, and can display distinct photophysiological status. We demonstrate how measured distributions of PSII quantum yields in plant tissue change under natural tissue light gradients as compared to conventionally measured quantum yields with even exposure to actinic light...
August 18, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28817694/a-new-holistic-3d-non-invasive-analysis-of-cellular-distribution-and-motility-on-fibroin-alginate-microcarriers-using-light-sheet-fluorescent-microscopy
#2
Serena Duchi, Filippo Piccinini, Michela Pierini, Alessandro Bevilacqua, Maria Luisa Torre, Enrico Lucarelli, Spartaco Santi
Cell interaction with biomaterials is one of the keystones to developing medical devices for tissue engineering applications. Biomaterials are the scaffolds that give three-dimensional support to the cells, and are vectors that deliver the cells to the injured tissue requiring repair. Features of biomaterials can influence the behaviour of the cells and consequently the efficacy of the tissue-engineered product. The adhesion, distribution and motility of the seeded cells onto the scaffold represent key aspects, and must be evaluated in vitro during the product development, especially when the efficacy of a specific tissue-engineered product depends on viable and functional cell loading...
2017: PloS One
https://www.readbyqxmd.com/read/28814672/vipar-a-quantitative-approach-to-3d-histopathology-applied-to-lymphatic-malformations
#3
René Hägerling, Dominik Drees, Aaron Scherzinger, Cathrin Dierkes, Silvia Martin-Almedina, Stefan Butz, Kristiana Gordon, Michael Schäfers, Klaus Hinrichs, Pia Ostergaard, Dietmar Vestweber, Tobias Goerge, Sahar Mansour, Xiaoyi Jiang, Peter S Mortimer, Friedemann Kiefer
BACKGROUND: Lack of investigatory and diagnostic tools has been a major contributing factor to the failure to mechanistically understand lymphedema and other lymphatic disorders in order to develop effective drug and surgical therapies. One difficulty has been understanding the true changes in lymph vessel pathology from standard 2D tissue sections. METHODS: VIPAR (volume information-based histopathological analysis by 3D reconstruction and data extraction), a light-sheet microscopy-based approach for the analysis of tissue biopsies, is based on digital reconstruction and visualization of microscopic image stacks...
August 17, 2017: JCI Insight
https://www.readbyqxmd.com/read/28807106/developmental-adaptations-of-trypanosome-motility-to-the-tsetse-fly-host-environments-unravel-a-multifaceted-in-vivo-microswimmer-system
#4
Sarah Schuster, Timothy Krüger, Ines Subota, Sina Thusek, Brice Rotureau, Andreas Beilhack, Markus Engstler
The highly motile and versatile protozoan pathogen Trypanosoma brucei undergoes a complex life cycle in the tsetse fly. Here we introduce the host insect as an expedient model environment for microswimmer research, as it allows examination of microbial motion within a diversified, secluded and yet microscopically tractable space. During their week-long journey through the different microenvironments of the fly´s interior organs, the incessantly swimming trypanosomes cross various barriers and confined surroundings, with concurrently occurring major changes of parasite cell architecture...
August 15, 2017: ELife
https://www.readbyqxmd.com/read/28793850/glycation-oxidation-and-glycoxidation-of-igg-a-biophysical-biochemical-immunological-and-hematological-study
#5
Sidra Islam, Moinuddin, Abdul Rouf Mir, Alok Raghav, Safia Habib, Khursheed Alam, Asif Ali
Glycation and oxidation induce structural alterations in the proteins in an inter-dependent manner with consequent pathological implications. The published literature presents wide range of modifications in conformational characteristics of proteins by glycation and oxidation; however, there is little data that could elaborate the cumulative effect of both the processes. This study has analysed the modifications in IgG by methylglyoxal (MG) (glycative stress), hydroxyl radical (OH(•)) (oxidative stress) and by their combined action i...
August 9, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28783507/an-investigation-of-cell-growth-and-detachment-from-thermoresponsive-physically-crosslinked-networks
#6
Deirdre Healy, Maria Nash, Alexander Gorleov, Kerry Thompson, Peter Dockery, Yury Rochev
The primary aim of this investigation was to determine the biocompatibility and cell culture potential of a newly designed class of thermoresponsive polymers. The attractiveness of these polymers lies in the fact that they swell rather than dissolve when the temperature is reduced below their respective lower critical solution temperature, due to the incorporation of octadecyl methacrylate (ODMA). The ODMA monomer acts as a physical crosslinker, preventing polymer dissolution upon temperature reduction. Two polymers were studied in this investigation poly(N isorpoylacrylamide (NIPAm)(99...
July 27, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28783315/localized-charge-transfer-in-two-dimensional-molybdenum-trioxide
#7
Vipin Kumar, Liang Liu, Viet Cuong Nguyen, Venkateswarlu Bhavanasi, Kaushik Parida, Daniel Mandler, Pooi See Lee
Molybdenum trioxide is an interesting inorganic system in which the empty 4d states have potential to hold extra electrons and therefore can change states from insulating opaque (MoO3) to colored semimetallic (HxMoO3). Here, we characterize the local electrogeneration and charge transfer of the synthetic layered two-dimensional 2D MoO3-II (a polymorph of MoO3 and analogous to α-MoO3) in response to two different redox couples, i.e., [Ru(NH3)6](3+) and [Fe(CN)6](3-) by scanning electrochemical microscopy (SECM)...
August 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28766344/cell-shaping-micropatterns-for-quantitative-super-resolution-microscopy-imaging-of-membrane-mechanosensing-proteins
#8
Anthony Fernandez, Markville Bautista, Ramunas Stanciauskas, Taerin Chung, Fabien Pinaud
Patterning cells on microcontact-printed substrates is a powerful approach to control cell morphology and introduce specific mechanical cues on a cell's molecular organization. Although global changes in cellular architectures caused by micropatterns can easily be probed with diffraction-limited optical microscopy, studying molecular reorganizations at the nanoscale demands micropatterned substrates that accommodate the optical requirements of single molecule microscopy techniques. Here, we developed a simple micropatterning strategy that provides control of cellular architectures and is optimized for nanometer accuracy single molecule tracking and three-dimensional super-resolution imaging of plasma and nuclear membrane proteins in cells...
August 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28760346/a-defect-in-the-mitochondrial-protein-mpv17-underlies-the-transparent-casper-zebrafish
#9
Gianluca D'Agati, Rosanna Beltre, Anna Sessa, Alexa Burger, Yi Zhou, Christian Mosimann, Richard M White
The casper strain of zebrafish is widely used in studies ranging from cancer to neuroscience. casper offers the advantage of relative transparency throughout adulthood, making it particularly useful for in vivo imaging by epifluorescence, confocal, and light sheet microscopy. casper was developed by selective breeding of two previously described recessive pigment mutants: 1) nacre, which harbors an inactivating mutation of the mitfa gene, rendering the fish devoid of pigmented melanocytes; and 2) roy orbison, a mutant with so-far unidentified genetic cause that lacks reflective iridophores...
July 28, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28753575/glioblastoma-and-glioblastoma-stem-cells-are-dependent-on-functional-mth1
#10
Linda Pudelko, Pegah Rouhi, Kumar Sanjiv, Helge Gad, Christina Kalderén, Andreas Höglund, Massimo Squatrito, Alberto J Schuhmacher, Steven Edwards, Daniel Hägerstrand, Ulrika Warpman Berglund, Thomas Helleday, Lars Bräutigam
Glioblastoma multiforme (GBM) is an aggressive form of brain cancer with poor prognosis. Cancer cells are characterized by a specific redox environment that adjusts metabolism to its specific needs and allows the tumor to grow and metastasize. As a consequence, cancer cells and especially GBM cells suffer from elevated oxidative pressure which requires antioxidant-defense and other sanitation enzymes to be upregulated. MTH1, which degrades oxidized nucleotides, is one of these defense enzymes and represents a promising cancer target...
July 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/28747660/4-dimensional-functional-profiling-in-the-convulsant-treated-larval-zebrafish-brain
#11
Matthew J Winter, Dylan Windell, Jeremy Metz, Peter Matthews, Joe Pinion, Jonathan T Brown, Malcolm J Hetheridge, Jonathan S Ball, Stewart F Owen, Will S Redfern, Julian Moger, Andrew D Randall, Charles R Tyler
Functional neuroimaging, using genetically-encoded Ca(2+) sensors in larval zebrafish, offers a powerful combination of high spatiotemporal resolution and higher vertebrate relevance for quantitative neuropharmacological profiling. Here we use zebrafish larvae with pan-neuronal expression of GCaMP6s, combined with light sheet microscopy and a novel image processing pipeline, for the 4D profiling of chemoconvulsant action in multiple brain regions. In untreated larvae, regions associated with autonomic functionality, sensory processing and stress-responsiveness, consistently exhibited elevated spontaneous activity...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28743899/thrombopoiesis-is-spatially-regulated-by-the-bone-marrow-vasculature
#12
David Stegner, Judith M M vanEeuwijk, Oğuzhan Angay, Maximilian G Gorelashvili, Daniela Semeniak, Jürgen Pinnecker, Patrick Schmithausen, Imke Meyer, Mike Friedrich, Sebastian Dütting, Christian Brede, Andreas Beilhack, Harald Schulze, Bernhard Nieswandt, Katrin G Heinze
In mammals, megakaryocytes (MKs) in the bone marrow (BM) produce blood platelets, required for hemostasis and thrombosis. MKs originate from hematopoietic stem cells and are thought to migrate from an endosteal niche towards the vascular sinusoids during their maturation. Through imaging of MKs in the intact BM, here we show that MKs can be found within the entire BM, without a bias towards bone-distant regions. By combining in vivo two-photon microscopy and in situ light-sheet fluorescence microscopy with computational simulations, we reveal surprisingly slow MK migration, limited intervascular space, and a vessel-biased MK pool...
July 25, 2017: Nature Communications
https://www.readbyqxmd.com/read/28724968/ce-3-ion-surface-oxygen-vacancy-and-visible-light-induced-photocatalytic-dye-degradation-and-photocapacitive-performance-of-ceo2-graphene-nanostructures
#13
Mohammad Ehtisham Khan, Mohammad Mansoob Khan, Moo Hwan Cho
Cerium oxide nanoparticles (CeO2 NPs) were fabricated and grown on graphene sheets using a facile, low cost hydrothermal approach and subsequently characterized using different standard characterization techniques. X-ray photoelectron spectroscopy and electron paramagnetic resonance revealed the changes in surface states, composition, changes in Ce(4+) to Ce(3+) ratio, and other defects. Transmission electron microscopy (TEM) and high resolution TEM revealed that the fabricated CeO2 NPs to be spherical with particle size of ~10-12 nm...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28717817/quantifying-three-dimensional-rodent-retina-vascular-development-using-optical-tissue-clearing-and-light-sheet-microscopy
#14
Jasmine N Singh, Taylor M Nowlin, Gregory J Seedorf, Steven H Abman, Douglas P Shepherd
No abstract text is available yet for this article.
July 1, 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28675620/site-specific-sers-assay-for-survivin-protein-dimer-from-ensemble-experiments-to-correlative-single-particle-imaging
#15
Jörg Wissler, Sandra Bäcker, Alessandro Feis, Shirley K Knauer, Sebastian Schlücker
An assay for Survivin, a small dimeric protein which functions as modulator of apoptosis and cell division and serves as a promising diagnostic biomarker for different types of cancer, is presented. The assay is based on switching on surface-enhanced Raman scattering (SERS) upon incubation of the Survivin protein dimer with Raman reporter-labeled gold nanoparticles (AuNP). Site-specificity is achieved by complexation of nickel-chelated N-nitrilo-triacetic acid (Ni-NTA) anchors on the particle surface by multiple histidines (His6 -tag) attached to each C-terminus of the centrosymmetric protein dimer...
July 4, 2017: Small
https://www.readbyqxmd.com/read/28671211/glucose-directs-amyloid-beta-into-membrane-active-oligomers
#16
Niraja Kedia, Michael Almisry, Jan Bieschke
Oligomeric amyloid-β 1-42 (Aβ-42) peptides are considered to be the most toxic species connected to the occurrence of Alzheimer's disease. However, not all aggregation conditions promote oligomer formation in vitro, raising the question whether oligomer formation in vivo also requires a specific suitable cellular environment. We recently found that interaction with neuronal membranes initiates aggregation of Aβ-42 and neuronal uptake. Our data suggest that small molecules in the extracellular space can facilitate the formation of membrane-active Aβ-42 oligomers...
July 21, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28661225/different-amyloid-aggregation-of-smooth-muscles-titin-in-vitro
#17
Elmira I Yakupova, Ivan M Vikhlyantsev, Liya G Bobyleva, Nikita V Penkov, Alexander A Timchenko, Maria A Timchenko, Gennady A Enin, Sergei S Khutzian, Olga M Selivanova, Alexander G Bobylev
A comparative study of amyloid properties of the aggregates of smooth muscle titin (SMT) from chicken gizzard was carried out. These aggregates were formed in two solutions: 0.15 M glycine-KOH, pH 7.2-7.4 (SMT(Gly)) and 0.2 M KCl, 10 mM imidazole, pH 7.0 (SMT(KCl)). Electron microscopy data showed that SMT aggregates has an amorphous structure in both cases. The results of atomic-force microscopy demonstrated slight differences in morphology in two types of aggregates. The SMT(Gly) aggregates were represented as branching chains, composed of spherical aggregates approximately 300-500 nm in diameter and up to 35 nm in height...
June 29, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28648678/single-plane-illumination-microscopy-as-a-tool-for-studying-nucleome-dynamics
#18
Jörg Langowski
Single plane illumination microscopy (SPIM) is a new optical method that has become extremely important in recent years. It is based on the formation of a "light slice" in the specimen in which fluorescently tagged molecules are observed. The spatial resolution is close to that of confocal optics, but without the disadvantages inherent to scanning or high laser irradiation doses. A recent development is light sheet fluctuation microscopy, which exploits the dynamic information contained in the fluorescence intensity fluctuations of each image pixel...
July 1, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28641108/high-speed-and-scalable-whole-brain-imaging-in-rodents-and-primates
#19
Kaoru Seiriki, Atsushi Kasai, Takeshi Hashimoto, Wiebke Schulze, Misaki Niu, Shun Yamaguchi, Takanobu Nakazawa, Ken-Ichi Inoue, Shiori Uezono, Masahiko Takada, Yuichiro Naka, Hisato Igarashi, Masato Tanuma, James A Waschek, Yukio Ago, Kenji F Tanaka, Atsuko Hayata-Takano, Kazuki Nagayasu, Norihito Shintani, Ryota Hashimoto, Yasuto Kunii, Mizuki Hino, Junya Matsumoto, Hirooki Yabe, Takeharu Nagai, Katsumasa Fujita, Toshio Matsuda, Kazuhiro Takuma, Akemichi Baba, Hitoshi Hashimoto
Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures...
June 21, 2017: Neuron
https://www.readbyqxmd.com/read/28637955/fine-structure-of-human-thoracic-duct-as-revealed-by-light-and-scanning-electron-microscopy
#20
Tomohiro Chiba, Hirokazu Narita, Hiroshi Shimoda
Little information has been available regarding microanatomy of human thoracic duct in spite of the importance for an understanding of pathophysiology in clinical medicine. The present study demonstrated a fine structure of human thoracic duct system by light and scanning electron microscopy. A number of longitudinal or spiral ridges and grooves were formed on luminal surfaces of the lymphangia and lymph sac, it likely facilitating fluent lymph flow. The endothelial cells displayed various cell shapes in compliance with their distributed regions...
2017: Biomedical Research
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