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Light sheet microscopy

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https://www.readbyqxmd.com/read/29342088/synthesis-and-characterization-of-wo%C3%A2-graphene-nanocomposites-for-enhanced-photocatalytic-activities-by-one-step-in-situ-hydrothermal-reaction
#1
Xiaoxiao Hu, Peiquan Xu, Hongying Gong, Guotao Yin
Tungsten trioxide (WO₃) nanorods are synthesized on the surface of graphene (GR) sheets by using a one-step in-situ hydrothermal method employing sodium tungstate (Na₂WO₄·2H₂O) and graphene oxide (GO) as precursors. The resulting WO₃/GR nanocomposites are characterized by X-ray diffraction, Raman spectroscopy, transmission electron microscopy, scanning electron microscopy and X-ray photoelectron spectroscopy. The results confirm that the interface between WO₃ nanorod and graphene contains chemical bonds...
January 17, 2018: Materials
https://www.readbyqxmd.com/read/29330427/three-dimensional-speckle-light-self-healing-based-imaging-system
#2
Danilo G Pires, Artur F Sonsin, Alcenísio J Jesus-Silva, Eduardo J S Fonseca
Recently new methodologies for imaging have been achieved making use of multiple light scattering. Here we present the self-healing effect using a speckled light field. We present an experiment that constitutes a useful application for a three-dimensional light sheet-based imaging system through an inhomogeneous medium. Each layer can be imaged independently of the others. The axial resolution basically depends on the coherence length, which can be sub-wavelength and controllable. This allows for a simple and direct technique for imaging through scattering layers with axial resolution improvement...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29322581/technical-implementations-of-light-sheet-microscopy
#3
REVIEW
Zagato Elisa, Brans Toon, Stefaan C De Smedt, Remaut Katrien, Neyts Kristiaan, Braeckmans Kevin
Fluorescence-based microscopy is among the most successful methods in biological studies. It played a critical role in the visualization of subcellular structures and in the analysis of complex cellular processes, and it is nowadays commonly employed in genetic and drug screenings. Among the fluorescence-based microscopy techniques, light sheet fluorescence microscopy (LSFM) has shown a quite interesting set of benefits. The technique combines the speed of epi-fluorescence acquisition with the optical sectioning capability typical of confocal microscopes...
January 11, 2018: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29317629/3d-single-molecule-super-resolution-microscopy-with-a-tilted-light-sheet
#4
Anna-Karin Gustavsson, Petar N Petrov, Maurice Y Lee, Yoav Shechtman, W E Moerner
Tilted light sheet microscopy with 3D point spread functions (TILT3D) combines a novel, tilted light sheet illumination strategy with long axial range point spread functions (PSFs) for low-background, 3D super-localization of single molecules as well as 3D super-resolution imaging in thick cells. Because the axial positions of the single emitters are encoded in the shape of each single-molecule image rather than in the position or thickness of the light sheet, the light sheet need not be extremely thin. TILT3D is built upon a standard inverted microscope and has minimal custom parts...
January 9, 2018: Nature Communications
https://www.readbyqxmd.com/read/29316331/light-sheet-microscopy-and-sral2-o4-nanoparticles-co-doped-with-eu2-dy3-ions-for-cancer-cell-tagging
#5
B Can-Uc, J B Montes-Frausto, K Juarez-Moreno, J Licea-Rodriguez, I Rocha-Mendoza, G A Hirata
Light sheet optical microscopy on strontium aluminate nanoparticles (SrAl2 O4 NPs) co-doped with Eu2+ and Dy3+ were used for cancer cell tagging and tracking. The nanoparticles were synthesized by urea-assisted combustion with optimized percentage values of the two co-doping rare-earth ions for cell viability and for lower cytotoxic effects. The optical properties of these materials showed an excitation wide range of wavelengths (λexc = 254 ~ 460 nm), a broad emission band (λem = 475 ~ 575 nm) with the maximum centered wavelength at 525 nm and a half lifetime within the seconds regime...
January 9, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29311144/differentiation-and-characterization-of-excitatory-and-inhibitory-synapses-by-cryo-electron-tomography-and-correlative-microscopy
#6
Chang-Lu Tao, Yun-Tao Liu, Rong Sun, Bin Zhang, Lei Qi, Sakar Shivakoti, Chong-Li Tian, Peijun Zhang, Pak-Ming Lau, Z Hong Zhou, Guo-Qiang Bi
As key functional units in neural circuits, different types of neuronal synapses play distinct roles in brain information processing, learning and memory. Synaptic abnormalities are believed to underlie various neurological and psychiatric disorders. Here, by combining cryo-electron tomography and cryo-correlative light and electron microscopy, we distinguished intact excitatory and inhibitory synapses of cultured hippocampal neurons, and visualized the in situ three-dimensional organization of synaptic organelles and macromolecules in their native state...
January 8, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29297721/mucor-circinelloides-induces-platelet-aggregation-through-integrin-%C3%AE-iib%C3%AE-3-and-fc%C3%AE-riia
#7
Harlene Ghuman, Alicia Shepherd-Roberts, Stephanie Watson, Malou Zuidscherwoude, Steve P Watson, Kerstin Voelz
Thrombosis is a hallmark of the fatal fungal infection mucormycosis. Yet, the platelet activation pathway in response to mucormycetes is unknown. In this study we determined the platelet aggregation potential of Mucor circinelloides (M. circinelloides) NRRL3631, characterized the signaling pathway facilitating aggregation in response to fungal spores, and identified the influence of the spore developmental stage upon platelet aggregation potential. Using impedance and light-transmission aggregometry, we showed that M...
January 3, 2018: Platelets
https://www.readbyqxmd.com/read/29286002/cell-accurate-optical-mapping-across-the-entire-developing-heart
#8
Michael Weber, Nico Scherf, Alexander M Meyer, Daniela Panáková, Peter Kohl, Jan Huisken
Organogenesis depends on orchestrated interactions between individual cells and morphogenetically relevant cues at the tissue level. This is true for the heart, whose function critically relies on well-ordered communication between neighboring cells, which is established and fine-tuned during embryonic development. For an integrated understanding of the development of structure and function, we need to move from isolated snap-shot observations of either microscopic or macroscopic parameters to simultaneous and, ideally continuous, cell-to-organ scale imaging...
December 29, 2017: ELife
https://www.readbyqxmd.com/read/29283513/oblique-scanning-two-photon-light-sheet-fluorescence-microscopy-for-rapid-volumetric-imaging
#9
Younghoon Shin, Dongmok Kim, Hyuk-Sang Kwon
Light-sheet fluorescence microscopy (LSFM) is a powerful tool for biological studies because it allows for optical sectioning of dynamic samples with superior temporal resolution. However, LSFM using two orthogonally co-aligned objectives requires a special sample geometry, and volumetric imaging speed is limited due to physical sample translation. This paper describes an oblique scanning two-photon LSFM (OS-2P-LSFM) that eliminates these limitations by using a single objective near the sample and a refractive scanning-descanning system...
December 28, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29245793/three-dimensional-live-multi-label-light-sheet-imaging-with-synchronous-excitation-multiplexed-structured-illumination
#10
Dongli Xu, Weibin Zhou, Leilei Peng
Multiplexed imaging is a powerful tool for studying complex interactions inside biological systems. Spectral imaging methods that capture multiple fluorescent markers synchronously without sacrificing the imaging speed or resolution are most suitable for live imaging. We describe spectral-encoded structured illumination (spectral-SIM) light-sheet microscopy, which enables parallel multi-excitation-channel imaging in 3D. Spectral-SIM encodes the excitation wavelength as the phase of the illumination pattern, and allows synchronous image capture over multiple excitation channels at the same speed and spatial resolution as mono-channel structured light-sheet imaging...
December 11, 2017: Optics Express
https://www.readbyqxmd.com/read/29221856/light-triggered-dissociation-of-self-assembled-%C3%AE-amyloid-aggregates-into-small-nontoxic-fragments-by-ruthenium-ii-complex
#11
Giyeong Son, Byung Il Lee, You Jung Chung, Chan Beum Park
The self-assembly of β-amyloid (A β) peptides into highly stable plaques is a major hallmark of Alzheimer's disease. Here, we report visible light-driven dissociation of β -sheet-rich A β aggregates into small, nontoxic fragments using ruthenium (II) complex {[Ru(bpy)3]2+} that functions as a highly sensitive, biocompatible, photoresponsive anti-A β agent. According to our multiple analyses using thioflavin T, bicinchoninic acid, dynamic light scattering, atomic force microscopy, circular dichroism, and Fourier transform infrared spectroscopy, [Ru(bpy)3]2+ successfully disassembled A β aggregates by destabilizing the β -sheet secondary structure under illumination of white light-emitting diode light...
December 5, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29210380/construction-of-magnetically-separable-nial-ldh-fe3o4-rgo-nanocomposites-with-enhanced-photocatalytic-performance-under-visible-light
#12
Jie Ni, Jinjuan Xue, LinFang Xie, Jing Shen, Guangyu He, Haiqun Chen
Magnetic NiAl layered doubled hydroxide (LDH)/Fe3O4-RGO composites were successfully synthesized via a simple hydrothermal route. The as-prepared samples were well characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). The results showed that NiAl LDH nanoplatelets and Fe3O4 nanoparticles sized around 15 nm were uniformly anchored on the surface of graphene sheets. The NiAl LDH/Fe3O4-RGO25 photocatalyst was employed to degrade ciprofloxacin (CIP) in an aqueous solution under visible light irradiation...
December 6, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29202458/integrating-light-sheet-imaging-with-virtual-reality-to-recapitulate-developmental-cardiac-mechanics
#13
Yichen Ding, Arash Abiri, Parinaz Abiri, Shuoran Li, Chih-Chiang Chang, Kyung In Baek, Jeffrey J Hsu, Elias Sideris, Yilei Li, Juhyun Lee, Tatiana Segura, Thao P Nguyen, Alexander Bui, René R Sevag Packard, Peng Fei, Tzung K Hsiai
Currently, there is a limited ability to interactively study developmental cardiac mechanics and physiology. We therefore combined light-sheet fluorescence microscopy (LSFM) with virtual reality (VR) to provide a hybrid platform for 3D architecture and time-dependent cardiac contractile function characterization. By taking advantage of the rapid acquisition, high axial resolution, low phototoxicity, and high fidelity in 3D and 4D (3D spatial + 1D time or spectra), this VR-LSFM hybrid methodology enables interactive visualization and quantification otherwise not available by conventional methods, such as routine optical microscopes...
November 16, 2017: JCI Insight
https://www.readbyqxmd.com/read/29200255/few-layer-wse2-schottky-junction-based-photovoltaic-devices-through-site-selective-dual-doping
#14
Seungpil Ko, Junhong Na, Young-Sun Moon, Ute Zschieschang, Rachana Acharya, Hagen Klauk, Gyu-Tae Kim, Marko Burghard, Klaus Kern
Ultrathin sheets of two-dimensional (2D) materials like transition metal dichalcogenides have attracted strong attention as components of high-performance light-harvesting devices. Here, we report the implementation of Schottky junction-based photovoltaic devices through site-selective surface doping of few-layer WSe2 in lateral contact configuration. Specifically, whereas the drain region is covered by a strong molecular p-type dopant (NDP-9) to achieve an Ohmic contact, the source region is coated with an Al2O3 layer, which causes local n-type doping and correspondingly an increase of the Schottky barrier at the contact...
December 4, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29192250/coupling-optogenetics-and-light-sheet-microscopy-a-method-to-study-wnt-signaling-during-embryogenesis
#15
Prameet Kaur, Timothy E Saunders, Nicholas S Tolwinski
Optogenetics allows precise, fast and reversible intervention in biological processes. Light-sheet microscopy allows observation of the full course of Drosophila embryonic development from egg to larva. Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo. To develop this method, we investigated the regulation of canonical Wnt signaling during anterior-posterior patterning of the Drosophila embryonic epidermis. Cryptochrome 2 (CRY2) from Arabidopsis Thaliana was fused to mCherry fluorescent protein and Drosophila β-catenin to form an easy to visualize optogenetic switch...
November 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29175679/monitoring-microbial-communities-using-light-sheet-fluorescence-microscopy
#16
REVIEW
Raghuveer Parthasarathy
Microbes often live in dense, dynamic, multi-species communities whose architecture and function are intimately intertwined. Imaging these complex, three-dimensional ensembles presents considerable technical challenges, however. In this review, I describe light sheet fluorescence microscopy, a technique that enables rapid acquisition of three-dimensional images over large fields of view and over long durations, and I highlight recent applications of this method to microbial systems that include artificial closed ecosystems, bacterial biofilms, and gut microbiota...
November 22, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29168380/quantitative-study-of-oligomerization-of-yeast-prion-sup35nm-proteins
#17
Yanjing Wang, Chi Wu
The fibrillation of misfolded neurodegenerative diseases related proteins has been extensively studied in the last few decades, but the initial oligomerization has been rarely touched even though some recent evidences indicated that small protein oligomers are more neurotoxic than long protein fibers. It is rather difficult to study the initial oligomerization (nucleation) because most of experimental methods, such as β-sheet related fluorescence assay and X-ray diffraction, are helpless in detecting small structure-less protein oligomers...
November 23, 2017: Biochemistry
https://www.readbyqxmd.com/read/29168308/demonstrating-improved-multiple-transport-mean-free-path-imaging-capabilities-of-light-sheet-microscopy-in-the-quantification-of-fluorescence-dynamics
#18
Matthias Rieckher, Stylianos Psycharakis, Daniele Ancora, Evangelos Liapis, Athanasios Zacharopoulos, Jorge Ripoll, Nektarios Tavernarakis, Giannis Zacharakis
Optical microscopy constitutes one of the most fundamental paradigms for the understanding of complex biological mechanisms in the whole-organism and live-tissue context. Novel imaging techniques such as light sheet fluorescence microscopy (LSFM) and optical projection tomography (OPT) combined with phase-retrieval algorithms (PRT) can produce highly-resolved 3D images in multiple transport-mean-free-path scales. Our study aims to exemplify the microscopic capabilities of LSFM when imaging protein dynamics in Caenorhabditis elegans and the distribution of necrotic cells in cancer cell spheroids...
November 23, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/29163595/alfalfa-root-growth-rate-correlates-with-progression-of-microtubules-during-mitosis-and-cytokinesis-as-revealed-by-environmental-light-sheet-microscopy
#19
Petra Vyplelová, Miroslav Ovečka, Jozef Šamaj
Cell division and expansion are two fundamental biological processes supporting indeterminate root growth and development of plants. Quantitative evaluations of cell divisions related to root growth analyses have been performed in several model crop and non-crop plant species, but not in important legume plant Medicago sativa. Light-sheet fluorescence microscopy (LSFM) is an advanced imaging technique widely used in animal developmental biology, providing efficient fast optical sectioning under physiological conditions with considerably reduced phototoxicity and photobleaching...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29160058/unraveled-face-dependent-effects-of-multi-layered-graphene-embedded-in-transparent-organic-light-emitting-diodes
#20
Jong Tae Lim, Jaesu Kim, Hyunkoo Lee, Jaehyun Moon, Byoung-Hwa Kwon, Seongdeok Ahn, Nam Sung Cho, Byung-Wook Ahn, Jeong-Ik Lee, Kyuwook Ihm, Seong Chu Lim
With increasing demand for transparent conducting electrode, graphene has attracted considerable attention, owing to its high electrical conductivity, high transmittance, low reflectance, flexibility and tunable work function. Two faces of single layer graphene are indistinguishable in its nature and this idea has not been doubted even in multilayered graphene (MLG) because it is difficult to separately characterize the front (first-born) and rear face (last-born) of MLG by using conventional analysis tools, such as Raman, ultraviolet spectroscopy, scanning probe microscopy, and sheet resistance...
November 21, 2017: ACS Applied Materials & Interfaces
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