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Light sheet microscopy

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https://www.readbyqxmd.com/read/28208588/depicting-binding-mediated-translocation-of-hiv-1-tat-peptides-in-living-cells-with-nanoscale-pens-of-tat-conjugated-quantum-dots
#1
Chien Y Lin, Jung Y Huang, Leu-Wei Lo
Cell-penetrating peptides (CPPs) can translocate across cell membranes, and thus have great potential for the cellular delivery of macromolecular cargoes. However, the mechanism of this cellular uptake process is not yet fully understood. In this study, a time-lapse single-particle light-sheet microscopy technique was implemented to obtain a parallel visualization of the translocating process of individual human immunodeficiency virus 1 (HIV-1) transactivator of transcription (Tat) peptide conjugated quantum dots (TatP-QDs) in complex cellular terrains...
February 10, 2017: Sensors
https://www.readbyqxmd.com/read/28194956/direct-imaging-of-single-plasmonic-metal-nanoparticles-in-capillary-with-laser-light-sheet-scattering-imaging
#2
Xuan Cao, Jingjing Feng, Qi Pan, Bin Xiong, Yan He, Edward S Yeung
Understanding the heterogeneous distribution of the physical and chemical properties of plasmonic metal nanoparticles is fundamentally important to their basic and applied research. Traditionally, they are obtained either indirectly via bulk spectroscopic measurements plus electron microscopic characterizations, or through single molecule/particle imaging of nanoparticles immobilized on planar substrates. In this study, by using light-sheet scattering microscopy with a supercontinuum white laser, highly sensitive imaging of individual MNPs flowing inside a capillary, driven by either pressure or electric field, was achieved for the first time...
February 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28165052/light-sheet-fluorescence-imaging-to-localize-cardiac-lineage-and-protein-distribution
#3
Yichen Ding, Juhyun Lee, Jianguo Ma, Kevin Sung, Tomohiro Yokota, Neha Singh, Mojdeh Dooraghi, Parinaz Abiri, Yibin Wang, Rajan P Kulkarni, Atsushi Nakano, Thao P Nguyen, Peng Fei, Tzung K Hsiai
Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm(3) in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution...
February 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28132409/selectable-light-sheet-uniformity-using-tuned-axial-scanning
#4
Martí Duocastella, Craig B Arnold, Jason Puchalla
Light-sheet fluorescence microscopy (LSFM) is an optical sectioning technique capable of rapid three-dimensional (3D) imaging of a wide range of specimens with reduced phototoxicity and superior background rejection. However, traditional light-sheet generation approaches based on elliptical or circular Gaussian beams suffer an inherent trade-off between light-sheet thickness and area over which this thickness is preserved. Recently, an increase in light-sheet uniformity was demonstrated using rapid biaxial Gaussian beam scanning along the lateral and beam propagation directions...
February 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28118055/the-role-of-mitochondria-oxidative-stress-and-the-radical-binding-protein-a1m-in-cultured-porcine-retina
#5
Bo Åkerström, Martin Cederlund, Jesper Bergwik, Oscar Manouchehrian, Karin Arnér, Ingrid Holmgren Taylor, Fredrik Ghosh, Linnéa Taylor
PURPOSE: The purpose of this study was to explore the relationship between oxidative stress, antioxidant defense, mitochondrial structure, and biomechanical tissue support in the isolated porcine retina. METHODS: Full-thickness retinal sheets were isolated from adult porcine eyes. Retinas were cultured for 2 or 48 h using (1) a previously established low-support explant protocol with photoreceptors positioned against the culture membrane (porous polycarbonate) or (2) a high-support procedure developed by our group, apposing the Müller cell endfeet and inner limiting membrane against the membrane...
January 24, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28108412/deciphering-the-mechanistic-insight-into-the-stoichiometric-ratio-dependent-behavior-of-cu-ii-on-bsa-fibrillation
#6
Ajeet Singh, Poulami Datta, Lalit M Pandey
Various metal ions are recently implicated in protein aggregates and are associated with numerous neurodegenerative diseases. In the present work, we have scrutinized the effect of stoichiometric variation of Cu(II) on BSA fibrillation at physiological pH 7.4 through Thioflavin-T dye binding study, residual protein concentration, Fourier Transform Infrared spectroscopy (FTIR), Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) and Isothermal Titration Calorimetry (ITC). Fibrillation kinetics was studied through ThT fluorescence, which illustrated dependency on stoichiometric ratio of Cu(II)...
April 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28106870/an-autopilot-platform-for-high-resolution-light-sheet-microscopy
#7
Dustin M Graham
No abstract text is available yet for this article.
January 20, 2017: Lab Animal
https://www.readbyqxmd.com/read/28103010/a-fluorescent-hsp90-probe-demonstrates-the-unique-association-between-extracellular-hsp90-and-malignancy-in-vivo
#8
Lauren B Crowe, Philip F Hughes, David A Alcorta, Takuya Osada, Aaron P Smith, Juliane Totzke, David R Loiselle, Isaac D Lutz, Madhusudhana Gargesha, Debasish Roy, Jose Roques, David Darr, H Kim Lyerly, Neil L Spector, Timothy A J Haystead
Extracellular expression of heat shock protein 90 (eHsp90) by tumor cells is correlated with malignancy. Development of small molecule probes that can detect eHsp90 in vivo may therefore have utility in the early detection of malignancy. We synthesized a cell impermeable far-red fluorophore-tagged Hsp90 inhibitor to target eHsp90 in vivo. High resolution confocal and lattice light sheet microscopy show that probe-bound eHsp90 accumulates in punctate structures on the plasma membrane of breast tumor cells and is actively internalized...
February 23, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28102196/whole-brain-3d-mapping-of-human-neural-transplant-innervation
#9
Jonas Doerr, Martin Karl Schwarz, Dirk Wiedermann, Anke Leinhaas, Alina Jakobs, Florian Schloen, Inna Schwarz, Michael Diedenhofen, Nils Christian Braun, Philipp Koch, Daniel A Peterson, Ulrich Kubitscheck, Mathias Hoehn, Oliver Brüstle
While transplantation represents a key tool for assessing in vivo functionality of neural stem cells and their suitability for neural repair, little is known about the integration of grafted neurons into the host brain circuitry. Rabies virus-based retrograde tracing has developed into a powerful approach for visualizing synaptically connected neurons. Here, we combine this technique with light sheet fluorescence microscopy (LSFM) to visualize transplanted cells and connected host neurons in whole-mouse brain preparations...
January 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28081110/light-sheet-microscopy-in-a-glass-capillary-feedback-holographic-control-for-illumination-beam-correction
#10
Tobias Meinert, Benjamin Alexander Gutwein, Alexander Rohrbach
Light-sheet microscopy enables fast 3D, high-contrast imaging in biology and colloidal sciences. Recently, the controlled transport of living embryos or small colloids through stable glass capillaries is manifold interesting. Although they hardly impair the sample, glass capillaries spoil the image by generating significant aberrations of the illumination and detection light. Here, we analyze the deflection of illuminating Bessel beams at the capillary by k-spectral shifting, and correct for it by a beam deflector...
January 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28076804/3d-protein-dynamics-in-the-cell-nucleus
#11
Anand P Singh, Rémi Galland, Megan L Finch-Edmondson, Gianluca Grenci, Jean-Baptiste Sibarita, Vincent Studer, Virgile Viasnoff, Timothy E Saunders
The three-dimensional (3D) architecture of the cell nucleus plays an important role in protein dynamics and in regulating gene expression. However, protein dynamics within the 3D nucleus are poorly understood. Here, we present, to our knowledge, a novel combination of 1) single-objective based light-sheet microscopy, 2) photoconvertible proteins, and 3) fluorescence correlation microscopy, to quantitatively measure 3D protein dynamics in the nucleus. We are able to acquire >3400 autocorrelation functions at multiple spatial positions within a nucleus, without significant photobleaching, allowing us to make reliable estimates of diffusion dynamics...
January 10, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28075531/three-dimensional-imaging-flow-cytometry-through-light-sheet-fluorescence-microscopy
#12
REVIEW
Emilio J Gualda, Hugo Pereira, Gabriel G Martins, Rui Gardner, Nuno Moreno
Flow cytometry is the tool of choice for high-speed acquisition and analysis of large cell populations, with the tradeoff of lacking intracellular spatial information. Although in the last decades flow cytometry systems that can actually acquire two-dimensional spatial information were developed, some of the limitations remained though, namely constrains related to sample size and lack of depth or dynamic information. The combination of fluidics and light-sheet illumination has the potential to address these limitations...
February 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28060338/a-submerged-filter-paper-sandwich-for-long-term-ex-ovo-time-lapse-imaging-of-early-chick-embryos
#13
Manuel Schmitz, Ben K A Nelemans, Theodoor H Smit
Due to its availability, low cost, flat geometry, and transparency, the ex ovo chick embryo has become a major vertebrate animal model for the study of morphogenetic events, such as gastrulation(2), neurulation(3)(-)(5), somitogenesis(6), heart bending(7,8), and brain formation(9)(-)(13), during early embryogenesis. Key to understanding morphogenetic processes is to follow them dynamically by time-lapse imaging. The acquisition of time-lapse movies of chick embryogenesis ex ovo has been limited either to short time windows or to the need for an incubator to control temperature and humidity around the embryo(14)...
December 28, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28046474/th-ab-209-04-3d-light-sheet-luminescence-imaging-with-cherenkov-radiation
#14
P Bruza, H Lin, L Jarvis, D Gladstone, B Pogue
PURPOSE: To recover a three-dimensional density distribution of luminescent molecular probes located several centimeters deep within a highly scattering tissue. METHODS: We developed a novel sheet beam Cherenkov-excited luminescence scanned imaging (CELSI) methodology. The sample was irradiated by a horizontally oriented, vertically scanned 6 MV X-ray sheet beam (200mm × 5mm, 0.2mm vertical step) from a radiotherapy linear accelerator. The resulting Cherenkov light emission - and thus luminescent probe excitation - occurred exclusively along the irradiation plane due to a short diffusion path of secondary particles and Cherenkov photons...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28018729/volumetric-optical-mapping-in-early-embryonic-hearts-using-light-sheet-microscopy
#15
Pei Ma, Dennis C Chan, Shi Gu, Michiko Watanabe, Michael W Jenkins, Andrew M Rollins
Optical mapping (OM) of electrical activity using voltage-sensitive fluorescent dyes is a powerful tool for the investigation of embryonic cardiac electrophysiology. However, because conventional OM integrates the signal in depth and projects it to a two-dimensional plane, information acquired is incomplete and dependent upon the orientation of the sample. This complicates interpretation of data, especially when comparing one heart to another. To overcome this limitation, we present volumetric OM using light-sheet microscopy, which enables high-speed capture of optically sectioned slices...
December 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27999866/low-cost-multimodal-light-sheet-microscopy-for-optically-cleared-tissues-and-living-specimens
#16
Vincent Rouger, Ricardo Alchini, Alexei Kazarine, Angelica A Gopal, Marie-Pier Girouard, Alyson E Fournier, Paul W Wiseman
No abstract text is available yet for this article.
December 1, 2016: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/27977418/very-thin-ito-metal-mesh-hybrid-films-for-a-high-performance-transparent-conductive-layer-in-gan-based-light-emitting-diodes
#17
Jung-Hong Min, Hoe-Min Kwak, Kiyoung Kim, Woo-Lim Jeong, Dong-Seon Lee
In this paper, we introduce very thin Indium tin oxide (ITO) layers (5, 10, and 15 nm) hybridized with a metal mesh to produce high-performance transparent conductive layers (TCLs) in near-ultraviolet light-emitting diodes (NUV LEDs). Using UV-vis-IR spectrometry, Hall measurement, and atomic force microscopy, we found that 10 nm was the optimal thickness for the very thin ITO layers in terms of outstanding transmittance and sheet resistance values as well as stable contact properties when hybridized with the metal mesh...
January 27, 2017: Nanotechnology
https://www.readbyqxmd.com/read/27964754/imaging-the-mammary-gland-and-mammary-tumours-in-3d-optical-tissue-clearing-and-immunofluorescence-methods
#18
Bethan Lloyd-Lewis, Felicity M Davis, Olivia B Harris, Jessica R Hitchcock, Filipe C Lourenco, Mathias Pasche, Christine J Watson
BACKGROUND: High-resolution 3D imaging of intact tissue facilitates cellular and subcellular analyses of complex structures within their native environment. However, difficulties associated with immunolabelling and imaging fluorescent proteins deep within whole organs have restricted their applications to thin sections or processed tissue preparations, precluding comprehensive and rapid 3D visualisation. Several tissue clearing methods have been established to circumvent issues associated with depth of imaging in opaque specimens...
December 13, 2016: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/27939712/a-review-of-methods-for-analysing-insect-structures-the-role-of-morphology-in-the-age-of-phylogenomics
#19
REVIEW
Benjamin Wipfler, Hans Pohl, Margarita I Yavorskaya, Rolf G Beutel
Techniques currently used in insect morphology are outlined briefly. Scanning electron microscopy (SEM) and microphotography are used mainly for documenting external features, the former providing more information on tiny surface structures and the latter on coloration, transparency and degree of sclerotization. A broad spectrum of methods is now available for anatomical studies: histological serial sections, confocal laser scanning microscopy (CLSM), light-sheet fluorescence microscopy (LSFM), serial block-face scanning electron microscopy (SBFSEM), dual beam scanning electron microscopy (FIB-SEM), nuclear magnetic resonance imaging (NMRI), and μ-computed tomography (micro-CT)...
December 2016: Current Opinion in Insect Science
https://www.readbyqxmd.com/read/27939706/long-term-fluorescence-live-imaging-of-tribolium-castaneum-embryos-principles-resources-scientific-challenges-and-the-comparative-approach
#20
REVIEW
Frederic Strobl, Ernst Hk Stelzer
Light sheet-based fluorescence microscopy became an important tool in insect developmental biology due to its high acquisition speed, low photo-bleaching rate and the high survival probability of the specimens. Initially applied to document the embryogenesis of Drosophila melanogaster, it is now used to investigate the embryonic morphogenesis of emerging model organisms such as the red flour beetle Tribolium castaneum. Here, we discuss the principles of light sheet-based fluorescence microscopy and outline Tribolium as a model organism for developmental biology...
December 2016: Current Opinion in Insect Science
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