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https://www.readbyqxmd.com/read/29903309/-construction-of-lamp-detection-method-for-poppy-codeine-in-seasoning-powders
#1
Liu Yang, Yufei Chen, Lei Wang
OBJECTIVE: To establish the detection method for poppy codeine in seasoning power based on the loop-mediated isothermal amplification of DNA( LAMP). METHODS: Establishment of the LAMP reaction system and detection of 8 commercially seasoning powders by the specificity test, optimum temperature test and sensitivity test of DNA samples of 12 poppies seeds, 11 spices leaves or seeds and 1 corn poppy seeds based on the specific genes sequence of codeine. RESULTS: The DNA samples of the 12 poppies and the corn poppy showed the specific reaction, the optimum temperature was 66 ℃ and the minimum detectable concentration was 9 pg/μL, and the sensitivity was 100 times larger than that of PCR...
September 2017: Wei Sheng Yan Jiu, Journal of Hygiene Research
https://www.readbyqxmd.com/read/29402492/agrobacterium-bohemicum-sp-nov-isolated-from-poppy-seed-wastes-in-central-bohemia
#2
Jiří Zahradník, Jaroslav Nunvar, Hana Pařízková, Lucie Kolářová, Andrea Palyzová, Helena Marešová, Michal Grulich, Eva Kyslíková, Pavel Kyslík
Two non-pathogenic strains R89-1 and R90T isolated from poppy seed (Papaver somniferum L.) wastes were phenotypically and genotypically characterized. Multilocus sequence analysis (MLSA) was conducted with six genes (atpD, glnA, gyrB, recA, rpoB, 16S rRNA). The strains represented a new species which clustered with Agrobacterium rubi NBRC 13261T and Agrobacterium skierniewicense Ch11T type strains. MLSA was further accompanied by whole-genome phylogeny, in silico DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) analyses for both strains...
May 2018: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/29289495/mechanism-of-action-pharmacokinetics-efficacy-and-safety-of-transarterial-therapies-using-ethiodized-oil-preclinical-review-in-liver-cancer-models
#3
Ron C Gaba, Regina M Schwind, Sebastien Ballet
PURPOSE: To systematically review mechanism of action, pharmacokinetics (PKs), efficacy, and safety of ethiodized oil-based locoregional therapy (LRT) for liver cancer in preclinical models. MATERIALS AND METHODS: A MEDLINE search was performed from 1988 to 2016. Search terms included hepatocellular carcinoma (HCC), HCC, liver-cell carcinoma, liver, hepatic, hepatocarcinoma, transarterial or chemoembolization, TACE, animal, Lipiodol, Ethiodol, iodized oil, and/or poppy-seed oil...
March 2018: Journal of Vascular and Interventional Radiology: JVIR
https://www.readbyqxmd.com/read/29134077/investigation-of-the-acid-base-behaviour-of-the-opium-alkaloid-thebaine-in-lc-esi-ms-mobile-phase-by-nmr-spectroscopy
#4
Michelle G Carlin, John R Dean, Jonathan L Bookham, Justin J B Perry
As part of a research programme to establish an analytical method for the simultaneous detection of the five major opium alkaloids in poppy seeds by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) it was discovered that the inclusion of thebaine produced two peaks for the same compound. This was in contrast to the effective simultaneous detection, by LC-ESI-MS, of morphine, codeine, papaverine and noscapine. The presence of these two peaks for thebaine was investigated using nuclear magnetic resonance spectroscopy with deuterated solvents to emulate the mobile phase conditions experienced...
October 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/29037712/straightforward-analytical-method-to-determine-opium-alkaloids-in-poppy-seeds-and-bakery-products
#5
Patricia López, Diana P K H Pereboom-de Fauw, Patrick P J Mulder, Martien Spanjer, Joyce de Stoppelaar, Hans G J Mol, Monique de Nijs
A straightforward method to determine the content of six opium alkaloids (morphine, codeine, thebaine, noscapine, papaverine and narceine) in poppy seeds and bakery products was developed and validated down to a limit of quantification (LOQ) of 0.1mg/kg. The method was based on extraction with acetonitrile/water/formic acid, ten-fold dilution and analysis by LC-MS/MS using a pH 10 carbonate buffer. The method was applied for the analysis of 41 samples collected in 2015 in the Netherlands and Germany. All samples contained morphine ranging from 0...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29033916/contribution-of-ruminal-fungi-archaea-protozoa-and-bacteria-to-the-methane-suppression-caused-by-oilseed-supplemented-diets
#6
Shaopu Wang, Katrin Giller, Michael Kreuzer, Susanne E Ulbrich, Ueli Braun, Angela Schwarm
Dietary lipids can suppress methane emission from ruminants, but effects are variable. Especially the role of bacteria, archaea, fungi and protozoa in mediating the lipid effects is unclear. In the present in vitro study, archaea, fungi and protozoa were selectively inhibited by specific agents. This was fully or almost fully successful for fungi and protozoa as well as archaeal activity as determined by the methyl-coenzyme M reductase alpha subunit gene. Five different microbial treatments were generated: rumen fluid being intact (I), without archaea (-A), without fungi (-F), without protozoa (-P) and with bacteria only (-AFP)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28973785/quantification-of-morphine-codeine-and-thebaine-in-home-brewed-poppy-seed-tea-by-lc-ms-ms
#7
Deborah Powers, Stephen Erickson, Madeleine J Swortwood
Recently, medical examiners reported two cases of a 21-year-old male and 24-year-old male with high amounts of morphine in their blood at autopsy. It was suspected that the decedents ingested lethal amounts of morphine from home-brewed poppy seed tea. No studies to date have investigated opium alkaloid content extracted from poppy seeds by home-brewing methods. Various poppy seed products were purchased from online sources and extracted with four home-brewing methods representative of recipes found on drug user forums...
October 3, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28861252/florally-rich-habitats-reduce-insect-pollination-and-the-reproductive-success-of-isolated-plants
#8
Tracie M Evans, Stephen Cavers, Richard Ennos, Adam J Vanbergen, Matthew S Heard
Landscape heterogeneity in floral communities has the potential to modify pollinator behavior. Pollinator foraging varies with the diversity, abundance, and spatial configuration of floral resources. However, the implications of this variation for pollen transfer and ultimately the reproductive success of insect pollinated plants remains unclear, especially for species which are rare or isolated in the landscape. We used a landscape-scale experiment, coupled with microsatellite genotyping, to explore how the floral richness of habitats affected pollinator behavior and pollination effectiveness...
August 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28681463/confirmation-of-recent-heroin-abuse-accepting-the-challenge
#9
REVIEW
Alexandra Maas, Burkhard Madea, Cornelius Hess
Confirmation or exclusion of recent heroin consumption is still one of the major challenges for forensic and clinical toxicologists. A great variety of biomarkers is available for heroin abuse confirmation, including various opium alkaloids (e.g. morphine, codeine), street heroin impurities (e.g. 6-acetylcodeine (6-AC), noscapine, papaverine) as well as associated metabolites (e.g. 6-monoacetylmorphine (6-MAM), morphine glucuronides). Though, the presence of most of these biomarkers cannot solely be attributed to a previous heroin administration but can, among other things, also be due to consumption of poppy seed products ('poppy seed defense'), opium preparations or specific medications, respectively...
July 6, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28599092/antioxidant-and-proteinase-inhibitory-activities-of-selected-poppy-papaver-somniferum-l-genotypes
#10
Erik Krošlák, Tibor Maliar, Peter Nemeček, Jana Viskupičová, Mária Maliarová, Michaela Havrlentová, Ján Kraic
Poppy seeds (Papaver somniferum L.) belong to tasty food ingredients however, they should be considered also as valuable source of biologically active compounds. Content of selected metabolites, antioxidant and proteinase inhibitory activities were analyzed in vitro in extracts from seeds of fifteen poppy genotypes. Considerable variation in all parameters was detected within the set of analyzed poppy genotypes. The genotype Major expressed the highest antioxidant activity determined by all four methodological approaches (DPPH, ABTS, FRAP, RP)...
September 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28580502/in-vitro-bioavailability-of-calcium-magnesium-iron-zinc-and-copper-from-gluten-free-breads-supplemented-with-natural-additives
#11
J Regula, A Cerba, J Suliburska, A A Tinkov
The aim of this study was to measure the content of calcium, magnesium, iron, zinc, and copper and determine the bioavailability of these ingredients in gluten-free breads fortified with milk and selected seeds. Due to the increasing prevalence of celiac disease and mineral deficiencies, it has become necessary to produce food with higher nutritional values which maintains the appropriate product characteristics. This study was designed for gluten-free breads fortified with milk and seeds such as flax, poppy, sunflower seeds, pumpkin seeds or nuts, and flour with amaranth...
March 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/28543087/death-due-to-complications-of-bowel-obstruction-following-raw-poppy-seed-ingestion
#12
Leah M Schuppener, Robert F Corliss
Eating poppy seeds can cause a positive urine drug screen, but it is unknown whether ingesting large quantities can result in opiate intoxication or toxicity. A 54-year-old woman with intractable vomiting was found unresponsive at home and later pronounced dead. At autopsy, a cast-like large bowel obstruction composed of poppy seeds was identified. Postmortem blood morphine level was < 10 ng/mL. Cause of death was determined to be complications of a bowel obstruction secondary to poppy seed ingestion. Deaths related to eating poppy seeds have not been described in the literature...
March 2018: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28444823/dissociable-corticostriatal-circuits-underlie-goal-directed-vs-cue-elicited-habitual-food-seeking-after-satiation-evidence-from-a-multimodal-mri-study
#13
Henk van Steenbergen, Poppy Watson, Reinout W Wiers, Bernhard Hommel, Sanne de Wit
The present multimodal MRI study advances our understanding of the corticostriatal circuits underlying goal-directed vs. cue-driven, habitual food seeking. To this end, we employed a computerized Pavlovian-instrumental transfer paradigm. During the test phase, participants were free to perform learned instrumental responses (left and right key presses) for popcorn and Smarties outcomes. Importantly, prior to this test half of the participants had been sated on popcorn and the other half on Smarties - resulting in a reduced desirability of those outcomes...
July 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/28406315/selective-boron-delivery-by-intra-arterial-injection-of-bsh-wow-emulsion-in-hepatic-cancer-model-for-neutron-capture-therapy
#14
Hironobu Yanagie, Novriana Dewi, Syushi Higashi, Ichiro Ikushima, Koji Seguchi, Ryoji Mizumachi, Yuji Murata, Yasuyuki Morishita, Atsuko Shinohara, Shoji Mikado, Nakahiro Yasuda, Mitsuteru Fujihara, Yuriko Sakurai, Kikue Mouri, Masashi Yanagawa, Tomoya Iizuka, Minoru Suzuki, Yoshinori Sakurai, Shin-Ichiro Masunaga, Hiroki Tanaka, Takehisa Matsukawa, Kazuhito Yokoyama, Takashi Fujino, Koichi Ogura, Yasumasa Nonaka, Hirotaka Sugiyama, Tetsuya Kajiyama, Sho Yui, Ryohei Nishimura, Koji Ono, Sinichi Takamoto, Jun Nakajima, Minoru Ono, Masazumi Eriguchi, Kenichiro Hasumi, Hiroyuki Takahashi
OBJECTIVE: Boron neutron-capture therapy (BNCT) has been used to inhibit the growth of various types of cancers. In this study, we developed a (10)BSH-entrapped water-in-oil-in-water (WOW) emulsion, evaluated it as a selective boron carrier for the possible application of BNCT in hepatocellular carcinoma treatment. METHODS: We prepared the (10)BSH-entrapped WOW emulsion using double emulsification technique and then evaluated the delivery efficacy by performing biodistribution experiment on VX-2 rabbit hepatic tumour model with comparison to iodized poppy-seed oil mix conventional emulsion...
June 2017: British Journal of Radiology
https://www.readbyqxmd.com/read/28382919/-doping-relevant-substances-in-horse-feed
#15
C Herholz, N Zink, H Laska, M Gumpendobler, C Trolliet, S Probst
Horse feed material of Swiss and foreign production available on the Swiss market was tested with regard to possible contamination with 7 different alkaloids, atropine and colchicine. Twenty-eight feed samples as well as 2 poppy seed samples were analyzed. Out of 28 feed samples 18 were positive for prohibited substances in Equestrian sports. The concentration of prohibited substances was in none of the samples high enough to cause an effect on the body of the horse. The poppy seed sample, which was obtained by conventional harvesting and cleaning methods, contained a very high Morphine concentration compared to the sample which was harvested by hand...
April 2017: Schweizer Archiv Für Tierheilkunde
https://www.readbyqxmd.com/read/28188639/effect-of-unconventional-oilseeds-safflower-poppy-hemp-camelina-on-in-vitro-ruminal-methane-production-and-fermentation
#16
Shaopu Wang, Michael Kreuzer, Ueli Braun, Angela Schwarm
BACKGROUND: Dietary supplementation with oilseeds can reduce methane emission in ruminants, but only a few common seeds have been tested so far. This study tested safflower (Carthamus tinctorius), poppy (Papaver somniferum), hemp (Cannabis sativa), and camelina (Camelina sativa) seeds in vitro using coconut (Cocos nucifera) oil and linseed (Linum usitatissimum) as positive controls. RESULTS: All the tested oilseeds suppressed methane yield (mL g-1 dry matter, up to 21%) compared to the non-supplemented control when provided at 70 g oil kg-1 dry matter, and they were as effective as coconut oil...
August 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27882891/a-comparative-mass-spectrometric-study-of-fatty-acids-and-metals-in-some-seed-extracts
#17
COMPARATIVE STUDY
Sonia Niculina Suvar, R Bleiziffer, P Podea, A Iordache, C Voica, R Zgavarogea, M Culea
A major cause of cardiovascular diseases and cancer is diet content, so the optimization of micronutrients in food is very important. Omega-3 fatty acids supplementation for patients had beneficial effects on subjective global assessment score and metabolic profiles. Fatty acids content and the metal ions in different seeds (e.g. linseed, poppy, grape, hemp, nuts, pumpkin, sesame, watermelon, chia) recommended as food supplements, purchased on the Romanian market, were compared. Gas chromatography coupled to mass spectrometry (GC-MS) was used as an excellent technique for fatty acids identification and quantitation, and inductively coupled plasma mass spectrometer (ICP-MS) for analytical measurements of metals...
2016: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/27659342/gene-actions-for-yield-and-its-attributes-and-their-implications-in-the-inheritance-pattern-over-three-generations-in-opium-poppy-papaver-somniferum-l
#18
Brij K Mishra, R Mishra, S N Jena, Sudhir Shukla
The gene actions for yield and its attributes and their inheritance pattern based on five parameter model have been explored in four single crosses (NBIHT-5 × NBIHT-6, NBIHT-5 × NBMHT-1, NBMHT-1 × NBIHT-6 and NBMHT-2 × NBMHT-1) obtained using thebaine rich pure lines of opium poppy (Papaver somniferum L.) for three consecutive generations. All the traits showed nonallelic mode of interaction, however, dominance effect (h) was more pronounced for all the traits except thebaine and papaverine. The dominance × dominance (l) effects were predominant over additive × additive (i) for all traits in all the four crosses except for papaverine...
September 2016: Journal of Genetics
https://www.readbyqxmd.com/read/27565444/urinary-excretion-study-following-consumption-of-various-poppy-seed-products-and-investigation-of-the-new-potential-street-heroin-marker-atm4g
#19
Alexandra Maas, Michael Krämer, Konrad Sydow, Pai-Shan Chen, Torsten Dame, Frank Musshoff, Bernd W K Diehl, Burkhard Madea, Cornelius Hess
Discrimination between street heroin consumption and poppy seed ingestion represents a major toxicological challenge in daily routine work. Several difficulties associated with conventional street heroin markers originate from their versatile occurrence in various poppy seed products and medications, respectively, as well as to small windows of detection. A novel opportunity to overcome these hindrances is represented by the new potential street heroin marker acetylated-thebaine-4-metabolite glucuronide (ATM4G), originating from thebaine during street heroin synthesis followed by metabolic reactions after administration...
March 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/27332789/hypersensitivities-to-sesame-and-other-common-edible-seeds
#20
REVIEW
A Patel, S L Bahna
Several seeds have been increasingly incorporated in various food items, with consequent risk of hypersensitivity reactions that are often severe. Identification of the specific seed as the culprit is often not explored or is difficult to verify. In this article, we reviewed the English literature from January 1930 to March 2016 using PubMed and Google Scholar searching for publications relevant to hypersensitivity to common edible seeds, namely sesame, sunflower seed, poppy seed, pumpkin seed, flaxseed, and mustard seed...
October 2016: Allergy
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